SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_003555493.1 NCBI__GCF_000023185.1:WP_003555493.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 1 hits to proteins with known functional sites (download)

P07117 Sodium/proline symporter; Proline carrier; Proline permease; Propionate transporter from Escherichia coli (strain K12) (see 4 papers)
23% identity, 72% coverage: 11:365/491 of query aligns to 12:368/502 of P07117

query
sites
P07117

A

C

V

V

F

Y

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I

F

F

F

G

F

M

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L

L

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T

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A

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S

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R

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W

R

R

K

S

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T

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K

T

N

L

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A

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D

D

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D

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Y

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L

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G

G

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S

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A

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P

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G

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W

K

K

R

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K

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A

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D

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K

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C

L

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A

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G

Y

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M

F

G

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H

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A

A

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D

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H

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Q

R257 (≠ G263) mutation to C: Sodium-independent binding affinity for proline.
C281 (≠ L286) mutation to S: Does not affect proline uptake activity. Confers resistance to N-ethylmaleimide. Na(+)-dependent proline binding activity is similar to wild-type carrier.
C344 (≠ V341) mutation to S: Small decrease in proline uptake activity. Confers resistance to N-ethylmaleimide. Exhibits low Na(+)-dependent proline binding.
C349 (≠ A346) mutation to S: Does not affect proline uptake activity. Sensitive to N-ethylmaleimide. Na(+)-dependent proline binding activity is similar to wild-type carrier.

Sites not aligning to the query:

376 mutation R->E,Q: No change in activity.; R→K: Loss of activity.

Query Sequence

>WP_003555493.1 NCBI__GCF_000023185.1:WP_003555493.1
MTTDINGTALAVFIFFFVLVTVMGFVASRWRKPETLASIDEWGLGGRNFGTWITWFLVGG
DFYTAYTVIAVPALVYTVGAYGFFALPYTIVVYPFVFMVMPVLWKRAKDFGYVTAGDVVH
GQYGSRGLELAVAATGVIATMPYIALQLVGMTAVLKALGLHGELPLAIAFIVLALYTYSA
GLRAPALIAFVKDIMIYIVVIAAVALIPSKLGGYANVFASADAAFQAKGSGNLLLGGNQY
VAYATLALGSALAAFMYPHTLTGIFASNSGKTIRKNAIMLPAYTLLLGLLALLGYMGHAA
NLKLDSANDVVPTLFKTLFSGWFSGFAFAAIAIGALVPAAVMSIGAANLFTRNFWKAYVD
PDVSDAGEAKVAKITSLVVKIGALLVIIFLPTQFALDLQLLGGIWILQTLPALVFGLYTN
WFRAPALLAGWFVGFGGGTFLVWDAGWKPLHLISLGGEPFTVYTGLLALAANIAVAVVVN
ALLPAKAPARA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory