SitesBLAST
Comparing WP_004042115.1 NCBI__GCF_000337315.1:WP_004042115.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
5nofA Anthranilate phosphoribosyltransferase from thermococcus kodakaraensis (see paper)
30% identity, 85% coverage: 49:355/360 of query aligns to 29:318/325 of 5nofA
2gvqD Anthranilate phosphoribosyl-transferase (trpd) from s. Solfataricus in complex with anthranilate (see paper)
25% identity, 88% coverage: 30:345/360 of query aligns to 15:316/345 of 2gvqD
P50384 Anthranilate phosphoribosyltransferase; EC 2.4.2.18 from Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus) (see 2 papers)
25% identity, 88% coverage: 30:345/360 of query aligns to 15:316/345 of P50384
- T87 (= T102) binding 5-phospho-alpha-D-ribose 1-diphosphate
- K106 (≠ V123) mutation to Q: Affinity for phosphoribosylpyrophosphate is similar to that of the wild-type enzyme and catalytic efficiency dedreases only 10-fold.
- H107 (= H124) mutation to A: Limited effect on either affinity for anthranilate and catalytic efficiency. 300-fold decrease of the affinity for anthranilate, whereas catalytic efficiency remains nearly unchanged; when associated with A-178.
- S118 (≠ A136) binding 5-phospho-alpha-D-ribose 1-diphosphate
- H154 (≠ N173) mutation to A: Limited effect on either affinity for anthranilate and catalytic efficiency.
- R164 (= R183) mutation to A: Strong decrease of the affinity for anthranilate, although only a moderate 7-fold decrease in catalytic efficiency.
- D223 (= D245) mutation to N: Affinity for phosphoribosylpyrophosphate is similar to that of the wild-type enzyme and catalytic efficiency is unchanged.
- E224 (≠ D246) mutation to Q: Affinity for phosphoribosylpyrophosphate is similar to that of the wild-type enzyme and catalytic efficiency is unchanged.
1zxyA Anthranilate phosphoribosyltransferase from sulfolobus solfataricus in complex with prpp and magnesium (see paper)
25% identity, 88% coverage: 30:345/360 of query aligns to 15:316/344 of 1zxyA
- binding magnesium ion: D223 (= D245), E224 (≠ D246)
- binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: A78 (≠ C93), G79 (= G94), D83 (= D98), T87 (= T102), N89 (≠ A104), S91 (≠ V108), T92 (≠ A109), K106 (≠ V123), S118 (≠ A136), D223 (= D245), E224 (≠ D246)
1gxbA Anthranilate phosphoribosyltransferase in complex with pyrophosphate and magnesium (see paper)
25% identity, 88% coverage: 30:345/360 of query aligns to 15:312/339 of 1gxbA
5bo3B Bianthranilate-like inhibitor with 5 atom "line" and phosphonate "hook" fishing for hydrogen bond donors in mycobacterium tuberculosis anthranilate phosphoribosyltransferase (anprt; trpd)
31% identity, 96% coverage: 10:354/360 of query aligns to 2:315/325 of 5bo3B
4zojA Methylsulfanyl-containing inhibitor bound in the active site of mycobacterium tuberculosis anthranilate phosphoribosyltransferase (anprt; trpd)
31% identity, 96% coverage: 10:354/360 of query aligns to 2:332/341 of 4zojA
- active site: V82 (≠ C93)
- binding 4-methyl-2-{[2-methyl-6-(methylsulfanyl)phenyl]amino}benzoic acid: N114 (≠ D127), A155 (≠ Q169), P156 (= P170), H159 (≠ N173), Y162 (≠ I176), R169 (= R182), G182 (≠ E196)
- binding magnesium ion: S95 (≠ L106), D227 (= D245), E228 (≠ D246), E228 (≠ D246)
- binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: G83 (= G94), G85 (≠ N96), G86 (≠ Y97), N93 (≠ A104), S95 (≠ L106), T96 (≠ G107), K111 (≠ H124), R115 (= R128), A116 (≠ V129), A117 (≠ P130), S119 (≠ Q132)
3gbrA Anthranilate phosphoribosyl-transferase (trpd) double mutant d83g f149s from s. Solfataricus (see paper)
25% identity, 88% coverage: 30:345/360 of query aligns to 15:313/341 of 3gbrA
- binding manganese (ii) ion: S91 (≠ G107), D220 (= D245), E221 (≠ D246), E221 (≠ D246)
- binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: A78 (≠ G94), G79 (≠ A95), T80 (≠ N96), G81 (≠ Y97), N89 (≠ I105), V90 (≠ L106), S91 (≠ G107), T92 (≠ V108), K106 (≠ V123), G114 (≠ Q132)
4owsA Anthranilate phosphoribosyl transferase from mycobacterium tuberculosis in complex with 4-methylanthranilate, prpp and magnesium (see paper)
29% identity, 96% coverage: 10:354/360 of query aligns to 2:330/340 of 4owsA
4yi7A Anthranilate bound at active site of anthranilate phosphoribosyl transferase from acinetobacter (anprt; trpd)
23% identity, 92% coverage: 31:360/360 of query aligns to 17:325/331 of 4yi7A
4n93A Alternative substrates of mycobacterium tuberculosis anthranilate phosphoribosyl transferase (see paper)
31% identity, 96% coverage: 10:354/360 of query aligns to 3:336/346 of 4n93A
- active site: V83 (≠ C93)
- binding 2-amino-6-methylbenzoic acid: V83 (≠ C93), G84 (= G94), T85 (≠ A95), H113 (≠ S125), N115 (≠ D127), N115 (≠ D127), A156 (≠ Q169), P157 (= P170), H160 (≠ N173), Y163 (≠ I176), A167 (≠ L179), R170 (= R182), G183 (≠ E196), P184 (≠ T197)
- binding magnesium ion: S96 (≠ L106), D228 (= D245), E229 (≠ D246), E229 (≠ D246)
- binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: G84 (= G94), G86 (≠ N96), G87 (≠ Y97), N94 (≠ A104), S96 (≠ L106), T97 (≠ G107), K112 (≠ H124), A117 (≠ V129), A118 (≠ P130), S120 (≠ Q132), G123 (≠ D135)
4zokA Methylsulfonyl-containing inhibitor bound in the substrate capture site of mycobacterium tuberculosis anthranilate phosphoribosyltransferase (anprt; trpd)
31% identity, 96% coverage: 10:354/360 of query aligns to 2:333/342 of 4zokA
- active site: V82 (≠ C93)
- binding 4-methyl-2-{[2-methyl-6-(methylsulfonyl)phenyl]amino}benzoic acid: P156 (= P170), R163 (vs. gap), A166 (≠ L179), A167 (≠ W180)
- binding magnesium ion: S95 (≠ L106), E228 (≠ D246)
- binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: G86 (≠ Y97), N93 (≠ A104), S95 (≠ L106), T96 (≠ G107), K111 (≠ H124), A116 (≠ V129), A117 (≠ P130), S119 (≠ Q132), G122 (≠ D135)
4zofA Lobenzarit-like inhibitor bound in the active site of mycobacterium tuberculosis anthranilate phosphoribosyltransferase (anprt; trpd)
31% identity, 96% coverage: 10:354/360 of query aligns to 2:333/342 of 4zofA
- active site: V82 (≠ C93)
- binding 2-[(2-carboxy-5-nitrophenyl)amino]-3-methylbenzoic acid: G113 (= G126), N114 (≠ D127), A155 (≠ Q169), H159 (≠ N173), Y162 (≠ I176), R169 (= R182), G182 (≠ E196)
- binding magnesium ion: S95 (≠ L106), D227 (= D245), E228 (≠ D246), E228 (≠ D246)
- binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: G83 (= G94), G85 (≠ N96), G86 (≠ Y97), N93 (≠ A104), S95 (≠ L106), T96 (≠ G107), K111 (≠ H124), A116 (≠ V129), A117 (≠ P130), S119 (≠ Q132), G122 (≠ D135)
5c1rA Stereoisomer of prpp bound in the active site of mycobacterium tuberculosis anthranilate phosphoribosyl (anprt; trpd)
31% identity, 96% coverage: 10:354/360 of query aligns to 2:334/343 of 5c1rA
- active site: V82 (≠ C93)
- binding 5-O-[(R)-hydroxy(phosphonooxy)phosphoryl]-1-O-phosphono-alpha-D-ribofuranose: V82 (≠ C93), G83 (= G94), G86 (≠ Y97), N93 (≠ A104), S95 (≠ L106), T96 (≠ G107), K111 (≠ H124), R115 (= R128), A117 (≠ P130), S118 (≠ T131), S119 (≠ Q132)
- binding magnesium ion: D87 (= D98), V89 (≠ K100), S95 (≠ L106), E228 (≠ D246)
4n5vA Alternative substrates of mycobacterium tuberculosis anthranilate phosphoribosyl transferase (see paper)
31% identity, 96% coverage: 10:354/360 of query aligns to 4:337/347 of 4n5vA
- active site: V84 (≠ C93)
- binding 2-amino-4-fluorobenzoic acid: V84 (≠ C93), G85 (= G94), H114 (≠ S125), G115 (= G126), N116 (≠ D127), A157 (≠ Q169), A157 (≠ Q169), P158 (= P170), H161 (≠ N173), Y164 (≠ I176), R165 (vs. gap), A168 (≠ L179), R171 (= R182), G184 (≠ E196)
- binding magnesium ion: S97 (≠ L106), D229 (= D245), E230 (≠ D246), E230 (≠ D246)
- binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: G85 (= G94), G87 (≠ N96), G88 (≠ Y97), N95 (≠ A104), S97 (≠ L106), T98 (≠ G107), K113 (≠ H124), A119 (≠ P130), S120 (≠ T131), S121 (≠ Q132), G124 (≠ D135)
3r88B Anthranilate phosphoribosyltransferase (trpd) from mycobacterium tuberculosis (complex with inhibitor acs145) (see paper)
31% identity, 96% coverage: 10:354/360 of query aligns to 2:334/344 of 3r88B
- active site: V82 (≠ C93)
- binding 2-amino-4,5-dimethoxybenzoic acid: N114 (≠ D127), A155 (≠ Q169), P156 (= P170), H159 (≠ N173), Y162 (≠ I176), R163 (vs. gap), A166 (≠ L179)
- binding magnesium ion: S95 (≠ L106), D227 (= D245), E228 (≠ D246), E228 (≠ D246)
- binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: V82 (≠ C93), G83 (= G94), G85 (≠ N96), G86 (≠ Y97), N93 (≠ A104), S95 (≠ L106), T96 (≠ G107), K111 (≠ H124), N114 (≠ D127), A117 (≠ P130), S118 (≠ T131), S119 (≠ Q132)
4ownA Anthranilate phosphoribosyl transferase from mycobacterium tuberculosis in complex with 5-fluoroanthranilate, prpp and magnesium (see paper)
31% identity, 96% coverage: 10:354/360 of query aligns to 3:334/344 of 4ownA
- active site: V83 (≠ C93)
- binding 2-amino-5-fluorobenzoic acid: V83 (≠ C93), G84 (= G94), H113 (≠ S125), G114 (= G126), N115 (≠ D127), N115 (≠ D127), A153 (≠ Q169), P154 (= P170), H157 (≠ N173), Y160 (≠ I176), R161 (vs. gap), A164 (≠ L179), G180 (≠ E196)
- binding magnesium ion: S96 (≠ L106), D225 (= D245), E226 (≠ D246), E226 (≠ D246)
- binding pyrophosphate 2-: V83 (≠ C93), G84 (= G94), N94 (≠ A104), S96 (≠ L106), T97 (≠ G107), K112 (≠ H124)
5c7sA Prpp complexed with two mn2+ in the active site of mycobacterium tuberculosis anthranilate phosphoribosyltransferase (anprt; trpd)
31% identity, 96% coverage: 10:354/360 of query aligns to 3:335/344 of 5c7sA
- active site: V83 (≠ C93)
- binding manganese (ii) ion: G84 (= G94), S96 (≠ L106), D228 (= D245), E229 (≠ D246), E229 (≠ D246)
- binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: V83 (≠ C93), G84 (= G94), G86 (≠ N96), G87 (≠ Y97), N94 (≠ A104), S96 (≠ L106), T97 (≠ G107), K112 (≠ H124), N115 (≠ D127), S120 (≠ Q132)
4m0rA Trianthranilate-like analogue bound to anthranilate phosphoribosyltransferase (anprt; trpd). (see paper)
29% identity, 96% coverage: 10:354/360 of query aligns to 2:333/342 of 4m0rA
4owmA Anthranilate phosphoribosyl transferase from mycobacterium tuberculosis in complex with 3-fluoroanthranilate, prpp and magnesium (see paper)
30% identity, 96% coverage: 10:354/360 of query aligns to 2:336/346 of 4owmA
- active site: V82 (≠ C93)
- binding 2-azanyl-3-fluoranyl-benzoic acid: P156 (= P170), H159 (≠ N173), Y162 (≠ I176), R163 (vs. gap), A166 (≠ L179), R170 (= R183)
- binding magnesium ion: S95 (≠ L106), D227 (= D245), E228 (≠ D246), E228 (≠ D246)
- binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: G83 (= G94), G86 (≠ Y97), N93 (≠ A104), S95 (≠ L106), T96 (≠ G107), K111 (≠ H124), A117 (≠ P130), S118 (≠ T131), S119 (≠ Q132), G122 (≠ D135)
Query Sequence
>WP_004042115.1 NCBI__GCF_000337315.1:WP_004042115.1
MAVVESEFGEWPLKRLMTEVVGTGTKSAEDMTREQASEAMRRILAGEPDHTTLGAFWLAN
RWKHNNAEELAAYADVMAEHVEYAEPDADPVDCGANYDGKGTTAILGVAAGVVAAAAGTP
VVVHSGDRVPTQKQDAYKHVLDELGVRTELDPEESADMVDDTGFGFYYQPRFNPAIHALW
ERRDNMGVRTFVNTIETIANPANASVHLGSFYHLAFAKKMVNTFEASEHHDLDRVLMFQG
MEGYDDIRPGYTKVAEWTTGDDGETTFDDFDIETAEYGMDFEYDDLGVDEDDVAGDSAEI
TEAVLAGEREDRFADAVALNAALRIYAREDAETIDEGLEMARETIASGDAEAVLEDLRDF
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory