SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_004042365.1 NCBI__GCF_000337315.1:WP_004042365.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 12 hits to proteins with known functional sites (download)

P00903 Aminodeoxychorismate synthase component 2; ADC synthase; ADCS; 4-amino-4-deoxychorismate synthase component 2; Aminodeoxychorismate synthase, glutamine amidotransferase component; EC 2.6.1.85 from Escherichia coli (strain K12) (see paper)
43% identity, 94% coverage: 4:195/204 of query aligns to 2:185/187 of P00903

query
sites
P00903

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C79 (= C88) mutation to S: 10000-fold decrease in catalytic efficiency.
H168 (= H178) mutation to Q: Loss of activity.
E170 (= E180) mutation to A: 150-fold decrease in catalytic efficiency.; mutation to D: 4-fold decrease in catalytic efficiency.; mutation E->K,Q: Loss of activity.

8hx8A Crystal structure of 4-amino-4-deoxychorismate synthase from streptomyces venezuelae co-crystallized with chorismate (see paper)
45% identity, 95% coverage: 2:194/204 of query aligns to 4:187/673 of 8hx8A

query
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8hx8A

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binding magnesium ion: I175 (≠ V182), S177 (≠ T184)

Sites not aligning to the query:

binding magnesium ion: 521, 655, 658
binding tryptophan: 231, 232, 233, 241, 243, 458, 459, 460, 614

Q42565 Anthranilate synthase beta subunit 1, chloroplastic; Anthranilate synthase component 2-1; Anthranilate synthase, glutamine amidotransferase component 2-1; Protein TRYPTOPHAN BIOSYNTHESIS 4; Protein WEAK ETHYLENE INSENSITIVE 7; EC 4.1.3.27 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
40% identity, 96% coverage: 4:198/204 of query aligns to 75:267/276 of Q42565

query
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Q42565

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G150 (= G86) mutation to D: In trp4-1; no visible phenotype under normal growth conditions.
G176 (= G111) mutation to E: In wei7-2; insensitive to inhibition of root elongation by ethylene.

P00900 Anthranilate synthase component 2; AS; ASII; Anthranilate synthase, GATase component; Anthranilate synthase, glutamine amidotransferase component; EC 4.1.3.27 from Serratia marcescens (see 3 papers)
37% identity, 96% coverage: 1:196/204 of query aligns to 1:188/193 of P00900

query
sites
P00900

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M1 (= M1) modified: Initiator methionine, Removed
C84 (= C88) active site, Nucleophile; for GATase activity

1i7qB Anthranilate synthase from s. Marcescens (see paper)
37% identity, 95% coverage: 4:196/204 of query aligns to 3:187/192 of 1i7qB

query
sites
1i7qB

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active site: G58 (= G60), C83 (= C88), L84 (= L89), H169 (= H178), E171 (= E180)
binding glutamic acid: P55 (= P57), G56 (= G58), G58 (= G60), C83 (= C88), L84 (= L89), Q87 (≠ E92), H132 (= H137), S133 (= S138), L134 (= L139)

8hx9A Crystal structure of 4-amino-4-deoxychorismate synthase from streptomyces venezuelae with chorismate (see paper)
34% identity, 95% coverage: 2:194/204 of query aligns to 3:144/632 of 8hx9A

query
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8hx9A

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binding magnesium ion: S134 (≠ T184), D135 (≠ G185)

Sites not aligning to the query:

binding (3R,4R)-3-[(1-carboxyethenyl)oxy]-4-hydroxycyclohexa-1,5-diene-1-carboxylic acid: 453, 454, 455, 456, 547, 570, 590, 603, 604, 605, 606, 619, 623
binding tryptophan: 189, 190, 191, 199, 201, 419, 420, 421, 574, 575

7yc6A Crystal structure of d110p mutant of gatase subunit of methanocaldococcus jannaschii gmp synthetase
31% identity, 81% coverage: 38:202/204 of query aligns to 31:181/183 of 7yc6A

query
sites
7yc6A

N

N

T

T

A

T

S

P

L

L

D

E

E

E

I

I

R

E

-

S

D

N

L

K

D

E

P

V

D

K

A

G

I

I

V

I

I

L

S

S

P

G

G

G

P

P

G

D

H

I

P

E

K

K

N

-

D

-

R

-

D

-

V

-

G

-

V

A

T

K

N

N

D

C

V

I

L

D

T

I

E

A

L

L

S

N

T

A

E

K

I

L

P

P

T

I

L

L

G

G

V

I

C
|
C

L

L

G

G

L

H

E

Q

A

L

A

I

V

A

Y

L

A

A

Y

Y

G

G

T

E

I

V

G

G

H

R

A

A

P

A

E

L

P

T

I

-

H

-

G

-

K

K

A

V

F

Y

P

-

V

V

D
|
D

H

K

D

E

G

N

A

P

G

-

V

L

F

F

A

K

G

N

L

V

E

P

D

R

G

E

F

F

P

N

A

A

G

W

R

A

Y

S

H

H

S

K

L

D

V
x
E

A

V

T

K

D

K

V

V

P

P

D

E

C

G

F

F

D

E

V

I

S

L

A

A

T
x
H

T

S

D

D

H

-

D

-

G

-

E

I

A
x
C

L

Q

V

V

M

E

G

A

V

M

R

K

H

H

R

K

D

T

Y

K

P

P

I

I

E

Y

C

G

V

V

Q

Q

F

F

H
|
H

P

P

E

E

S

V

V

A

L

H

T

T

G

E

S

Y

G

G

H

N

G

E

V

I

V

L

R

K

N

N

F

F

L

-

T

C

A

K

V

V

A

C

G

G

F

Y

D

K

binding zinc ion: C76 (= C88), D101 (= D117), E123 (≠ V140), E123 (≠ V140), H136 (≠ T153), C140 (≠ A160), C140 (≠ A160), H158 (= H178)

2ywcA Crystal structure of gmp synthetase from thermus thermophilus in complex with xmp
31% identity, 76% coverage: 40:195/204 of query aligns to 33:181/475 of 2ywcA

query
sites
2ywcA

A

A

S

P

L

L

D

E

E

E

I

V

R

L

D

K

L

H

D

R

P

P

D

Q

A

A

I

L

V

I

I

L

S

S

P

G

G
|
G

P

P

G

-

H
x
R

P

S

K

V

N

F

D

D

R

P

D

D

V

A

G

P

V

R

T

P

N

D

D

P

V

R

L

L

T

-

E

-

L

F

S

S

T

S

E

G

I

L

P

P

T

L

L

L

G

G

V

I

C
|
C

L
x
Y

G

G

L

M

E

Q

A

L

V

A

Y

Q

A

E

Y

L

G

G

T

R

I

V

G

E

H

R

A

A

P

G

E

R

P

A

I

E

H

Y

G

G

K

K

A

A

F

L

P

L

V

T

D

R

H

H

D

E

G

G

A

P

G

-

V

L

F

F

A

R

G

G

L

L

E

E

D

G

G

E

F

V

P

Q

A

V

G

W

R

M

Y

S

H

H

S

Q

L

D

V

A

A

V

T

T

D

A

V

P

P

P

D

P

C

G

F

W

D

R

V

V

S

V

A

A

T

E

T

T

D

E

H

E

D

N

G

P

E

V

A

A

L

A

V

I

M

A

G

S

V

P

R

D

H

G

R

R

D

A

Y

Y

P

-

I

-

E

-

C

G

V

V

Q

Q

F

F

H
|
H

P

P

E
|
E

S

V

V

A

L

H

T

T

G

P

S

K

G

G

H

M

G

Q

V

I

V

L

R

E

N

N

F

F

L

L

active site: G51 (= G58), R53 (≠ H61), C78 (= C88), Y79 (≠ L89), H164 (= H178), E166 (= E180)

Sites not aligning to the query:

active site: 221, 343
binding xanthosine-5'-monophosphate: 288, 366, 367, 368, 408, 467, 471, 472, 473

5tw7F Crystal structure of a gmp synthase (glutamine-hydrolyzing) from neisseria gonorrhoeae
26% identity, 78% coverage: 42:200/204 of query aligns to 39:193/490 of 5tw7F

query
sites
5tw7F

L

L

D

D

E

E

I

I

R

K

D

A

L

F

D

N

P

P

D

K

A

G

I

I

V

I

I

L

S

S

P

G

G
|
G

P

P

G

N

-

S

-

V

-

Y

H

E

P

S

K

D

N

Y

D

Q

R

A

D

D

V

T

G

G

V

I

T

-

N

-

D

-

V

-

L

-

T

-

E

-

L

F

S

D

T

L

E

G

I

I

P

P

T

V

L

L

G

G

V

I

C
|
C

L
x
Y

G

G

L

M

E

Q

A

F

A

M

V

A

Y

H

A

H

Y

L

G

G

T

E

I

V

-

Q

-

P

G

G

H

N

A

Q

P

R

E

E

P

F

I

G

H

Y

G

A

K

Q

A

V

F

K

P

T

V

I

D

D

H

-

D

-

G

-

A

S

G

G

V

L

F

T

A

R

G

G

L

I

E

Q

D

D

G

D

F

A

P

P

-

N

-

T

-

L

-

D

A

V

G

W

R

M

Y

S

H

H

S

G

L

D

V

K

A

V

T

S

D

K

V

L

P

P

D

D

C

G

F

F

D

A

V

V

S

I

A

G

T

D

T

T

D

P

H

S

D

C

G

P

E

I

A

A

L

M

V

M

M

E

G

N

V

T

R

E

H

K

R

Q

D

F

Y

Y

P

G

I

I

E

-

C

-

V

-

Q

Q

F

F

H
|
H

P

P

E
|
E

S

V

V

T

L

H

T

T

G

K

S

Q

G

G

H

R

G

A

V

L

R

N

N

R

F

F

L

V

T

L

A

D

V

I

A

C

G

G

active site: G55 (= G58), C81 (= C88), Y82 (≠ L89), H171 (= H178), E173 (= E180)

Sites not aligning to the query:

active site: 229, 345
binding magnesium ion: 466, 469, 472

P49915 GMP synthase [glutamine-hydrolyzing]; GMP synthetase; Glutamine amidotransferase; EC 6.3.5.2 from Homo sapiens (Human) (see paper)
29% identity, 58% coverage: 83:200/204 of query aligns to 99:212/693 of P49915

query
sites
P49915

P

P

T

V

L

L

G

G

V

I

C
|
C

L

Y

G

G

L

M

E

Q

A

M

V

N

Y

K

A

V

Y

F

G

G

T

T

I

V

G

-

H

H

A

K

P

K

E

S

P

V

I

R

H

E

G

D

K

G

A

V

F

F

P

N

V

I

D

S

H

V

D

D

G

N

-

T

A

C

G

S

V

L

F

F

A

R

G

G

L

L

E

Q

D

K

G

E

F

E

P

V

A

V

G

L

R

L

Y

T

H

H

S

G

L

D

V

S

A

V

T

D

D

K

V

V

P

A

D

D

C

G

F

F

D

K

V

V

S

V

A

A

T

R

T

S

D

G

H

N

D

-

G

-

E

-

A

-

L

I

V

V

M

A

G

G

V

I

R

A

H

N

R

E

D

S

Y

K

P

K

I

L

E

Y

C

G

V

A

Q

Q

F

F

H
|
H

P

P

E
|
E

S

V

V

G

L

L

T

T

G

E

S

N

G

G

H

K

G

V

V

I

V

L

R

K

N

N

F

F

L

L

T

Y

A

D

V

I

A

A

G

G

C104 (= C88) active site, For GATase activity
H190 (= H178) active site, For GATase activity
E192 (= E180) active site, For GATase activity

Sites not aligning to the query:

337 binding XMP
522 binding XMP
610 binding XMP
685 binding XMP
691 binding XMP

1gpmA Escherichia coli gmp synthetase complexed with amp and pyrophosphate (see paper)
24% identity, 95% coverage: 3:195/204 of query aligns to 7:196/501 of 1gpmA

query
sites
1gpmA

R

R

L

I

V

L

V

I

V

L

D

D

N

F

F

G

D

S

S

Q

F

Y

T

T

Y

Q

N

L

L

V

V

A

E

R

Y

R

F

V

S

R

E

E

Q

L

T

G

V

V

E

Y

G

C

E

E

P

L

L

W

D

A

I

W

E

D

V

V

R

T

K

E

N

A

T

-

A

-

S

-

L

-

D

-

E

Q

I

I

R

R

D

D

L

F

D

N

P

P

D

S

A

G

I

I

V

I

I

L

S

S

P

G

G
|
G

P

P

G

E

H

S

P

T

K

T

N

E

D

E

R

N

D

S

V

P

G

R

V

A

T

P

N

Q

D

Y

V

V

L

F

T

-

E

-

L

-

S

E

T

A

E

G

I

V

P

P

T

V

L

F

G

G

V

V

C
|
C

L
x
Y

G

G

L

M

E

Q

A

T

A

M

V

A

Y

M

A

Q

Y

L

G

G

T

H

I

V

G

E

H

A

A

S

P

N

E

E

P

R

I

E

H

F

G

G

K

Y

A

A

F

Q

P

V

V

E

D

V

H

V

D

N

G

D

A

S

G

A

V

L

F

V

A

R

G

G

L

I

E

E

D

D

G

A

F

L

P

T

A

A

-

D

-

G

-

K

-

P

-

L

-

D

-

V

G

W

R

M

Y

S

H

H

S

G

L

D

V

K

A

V

T

T

D

A

V

I

P

P

D

S

C

D

F

F

D

I

V

T

S

V

A

A

T

S

T

T

D

E

H

S

D

C

G

P

E

F

A

A

L

I

V

M

M

A

G

N

V

E

R

E

H

K

R

R

D

F

Y

Y

P

G

I

-

E

-

C

-

V

V

Q

Q

F

F

H
|
H

P

P

E
|
E

S

V

V

T

L

H

T

T

G

R

S

Q

G

G

H

M

G

R

V

M

V

L

R

E

N

R

F

F

L

V

active site: G57 (= G58), C84 (= C88), Y85 (≠ L89), H179 (= H178), E181 (= E180)

Sites not aligning to the query:

active site: 237, 357
binding adenosine monophosphate: 231, 232, 233, 258, 313
binding pyrophosphate 2-: 233, 235, 236, 237, 238, 357

2vxoB Human gmp synthetase in complex with xmp (see paper)
29% identity, 58% coverage: 83:200/204 of query aligns to 77:187/658 of 2vxoB

query
sites
2vxoB

P

P

T

V

L

L

G

G

V

I

C
|
C

L
x
Y

G

G

L

M

E

Q

A

M

V

N

Y

K

A

V

Y

F

G

G

T

T

I

V

G

H

H

K

A

K

P

S

E

D

P

G

I

V

H

-

G

-

K

-

A

-

F

F

P

N

V

I

D

S

H

V

D

D

G

N

A

T

-

C

G

S

V

L

F

F

A

R

G

G

L

L

E

Q

D

K

G

E

F

E

P

V

A

V

G

L

R

L

Y

T

H

H

S

G

L

D

V

S

A

V

T

D

D

K

V

V

P

A

D

D

C

G

F

F

D

K

V

V

S

V

A

A

T

R

T

S

D

G

H

N

D

-

G

-

E

-

A

-

L

I

V

V

M

A

G

G

V

I

R

A

H

N

R

E

D

S

Y

K

P

K

I

L

E

Y

C

G

V

A

Q

Q

F

F

H
|
H

P

P

E
|
E

S

V

V

G

L

L

T

T

G

E

S

N

G

G

H

K

G

V

V

I

V

L

R

K

N

N

F

F

L

L

T

Y

A

D

V

I

A

A

G

G

active site: C82 (= C88), Y83 (≠ L89), H165 (= H178), E167 (= E180)

Sites not aligning to the query:

active site: 55, 223, 381
binding xanthosine-5'-monophosphate: 302, 348, 349, 404, 405, 406, 489, 575, 610, 650, 654, 655, 656

Query Sequence

>WP_004042365.1 NCBI__GCF_000337315.1:WP_004042365.1
MIRLVVVDNFDSFTYNLVEYFSEQTVEGEPLDIEVRKNTASLDEIRDLDPDAIVISPGPG
HPKNDRDVGVTNDVLTELSTEIPTLGVCLGLEAAVYAYGGTIGHAPEPIHGKAFPVDHDG
AGVFAGLEDGFPAGRYHSLVATDVPDCFDVSATTDHDGEALVMGVRHRDYPIECVQFHPE
SVLTGSGHGVVRNFLTAVAGFDVA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory