SitesBLAST

O32148 (S)-ureidoglycine--glyoxylate transaminase; UGXT; (S)-ureidoglycine--glyoxylate aminotransferase; Purine catabolism protein PucG; EC 2.6.1.112 from Bacillus subtilis (strain 168) (see paper)
41% identity, 100% coverage: 1:377/378 of query aligns to 15:405/416 of O32148

query
sites
O32148

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Q37 (≠ H23) mutation to H: 5-fold decrease in transamination activity.
K198 (= K185) modified: N6-(pyridoxal phosphate)lysine
N264 (≠ Y233) mutation to S: 9-fold decrease in transamination activity.; mutation to Y: Loss of transamination activity.

3kgxA Crystal structure of putative aminotransferase (aah25799.1) from mus musculus at 1.80 a resolution
41% identity, 94% coverage: 1:357/378 of query aligns to 22:378/383 of 3kgxA

query
sites
3kgxA

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binding magnesium ion: H290 (≠ A270), H294 (≠ G274), D367 (≠ S346)

3kgwB Crystal structure of putative aminotransferase (aah25799.1) from mus musculus at 1.65 a resolution
41% identity, 94% coverage: 1:357/378 of query aligns to 26:383/388 of 3kgwB

query
sites
3kgwB

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binding pyridoxal-5'-phosphate: S81 (≠ T56), G82 (= G57), H83 (≠ S58), W108 (≠ F83), S158 (≠ T133), D183 (= D159), V185 (= V161), K209 (= K185)

1j04A Structural mechanism of enzyme mistargeting in hereditary kidney stone disease in vitro (see paper)
39% identity, 94% coverage: 1:357/378 of query aligns to 23:382/387 of 1j04A

query
sites
1j04A

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binding (aminooxy)acetic acid: P25 (= P3), R357 (= R332)

P21549 Alanine--glyoxylate aminotransferase; AGT; Serine--pyruvate aminotransferase; SPT; EC 2.6.1.44; EC 2.6.1.51 from Homo sapiens (Human) (see 24 papers)
39% identity, 94% coverage: 1:357/378 of query aligns to 26:385/392 of P21549

query
sites
P21549

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R36 (= R11) to C: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177157
G41 (≠ M16) to E: in HP1; loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177168; to R: in HP1; associated in cis with L-11 and M-340; results in loss of protein stability; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; partial mitochondrial mistargeting; intraperoxisomal protein aggregation seen; dbSNP:rs121908523; to V: in HP1; reduced alanine--glyoxylate aminotransferase activity; no loss of dimerization; no effect on protein stability; dbSNP:rs180177168
G47 (= G22) to R: in HP1; associated in cis with L-11 and M-340; results in protein misfolding; decreased alanine--glyoxylate aminotransferase activity; reduced expression levels; reduced pyridoxal phosphate binding; reduced dimerization; reduced thermostability; increased propensity to aggregation; increased susceptibility to proteolytic degradation within the N-terminal region; mitochondrial mistargeting; exposure to pyridoxine can rescue the functionality by partially preventing aggregation and degradation and by redirecting all the protein to the peroxisome; dbSNP:rs180177173
G82 (= G57) to E: in HP1; abolishes alanine--glyoxylate aminotransferase activity by interfering with pyridoxal phosphate binding; dbSNP:rs121908522
W108 (≠ F83) to R: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; decreased protein stability; dbSNP:rs180177197
A112 (≠ M87) to D: in HP1; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; decreased protein stability; causes protein aggregation; dbSNP:rs796052061
L150 (≠ V125) to P: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177222
F152 (≠ G127) to I: in HP1; associated in cis with L-11 and M-340; results in protein destabilization; no loss of dimerization; decreased alanine--glyoxylate aminotransferase activity; loss of alanine--glyoxylate aminotransferase activity when associated with L-11 and M-340; mitochondrial mistargeting when associated with L-11 and M-340; dbSNP:rs121908524
G156 (≠ A131) to R: in HP1; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; decreased protein stability; dbSNP:rs121908530
S158 (≠ T133) to L: in HP1; loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177225
G161 (= G136) to C: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; reduced expression levels; decreased protein stability; protein aggregation seen in the cytosol with a decreased aggregation propensity in the presence of pyridoxal phosphate; reduced peroxisomal localization; dbSNP:rs180177227; to R: in HP1; loss of alanine--glyoxylate aminotransferase activity; reduced expression levels; decreased protein stability; protein aggregation seen in the cytosol with a decreased aggregation propensity in the presence of pyridoxal phosphate; loss of dimerization; dbSNP:rs180177227; to S: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; reduced expression levels; decreased protein stability; protein aggregation seen in the cytosol with a decreased aggregation propensity in the presence of pyridoxal phosphate; reduced peroxisomal localization; dbSNP:rs180177227
L166 (≠ V142) to P: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177230
G170 (≠ T146) to R: in HP1; associated in cis with L-11 and M-340; decrease in alanine--glyoxylate aminotransferase activity; loss of dimerization; partial loss of protein stability but protein stability increases in the presence of pyridoxal phosphate; causes protein aggregation; loss of alanine--glyoxylate aminotransferase activity in vitro when associated with L-11 and M-340; results in mitochondrial mistargeting when associated with L-11 and M-340; dbSNP:rs121908529
C173 (≠ A149) to Y: in HP1; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; decreased protein stability; causes protein aggregation; dbSNP:rs180177231
D183 (= D159) to N: in HP1; loss of alanine--glyoxylate aminotransferase activity; no loss of dimerization; no effect on protein stability; dbSNP:rs180177236
S187 (= S163) to F: in HP1; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization but improved dimerization in the presence of pyridoxal phosphate; decreased protein stability; dbSNP:rs180177238
I202 (≠ V178) to N: in HP1; uncertain significance; dbSNP:rs536352238
S205 (= S181) to P: in HP1; loss of alanine--glyoxylate aminotransferase activity; decreased protein stability; dbSNP:rs121908520
K209 (= K185) mutation to R: Affects pyridoxal phosphate binding; loss of alanine--glyoxylate aminotransferase activity.
S218 (= S194) to L: in HP1; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; no effect on protein stability; dbSNP:rs180177253
R233 (= R209) to C: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs121908526; to H: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs121908527
I244 (≠ L220) to T: in HP1; associated in cis with L-11 and M-340; results in protein misfolding; decreased alanine--glyoxylate aminotransferase activity in vitro; no loss of dimerization; partial mitochondrial mistargeting; loss of alanine--glyoxylate aminotransferase activity in vitro when associated with L-11 and M-340; dbSNP:rs121908525
C253 (≠ D229) to R: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177264
I279 (≠ V252) to T: in dbSNP:rs140992177
A280 (= A253) to V: in dbSNP:rs73106685
V326 (= V298) to I: in dbSNP:rs115057148
I340 (≠ L312) to M: in allele minor; associated in cis with L-11; no effect on alanine--glyoxylate aminotransferase activity in vitro; decreased specific alanine--glyoxylate aminotransferase activity in vitro when associated with L-11; loss of alanine--glyoxylate aminotransferase activity in vitro when associated with L-11 and I-152; loss of alanine--glyoxylate aminotransferase activity in vitro when associated with L-11 and R-170; loss of alanine--glyoxylate aminotransferase activity in vitro when associated with L-11 and T-244; results in mitochondrial mistargeting when associated with L-11 and R-170; dbSNP:rs4426527

Sites not aligning to the query:

9 T → N: no loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs115014558
11 P → L: in allele minor; associated in cis with M-340; decreased specific alanine--glyoxylate aminotransferase activity in vitro when associated with M-340; loss of alanine--glyoxylate aminotransferase activity in vitro when associated with I-152 and M-340; loss of alanine--glyoxylate aminotransferase activity in vitro when associated with R-170 and M-340; loss of alanine--glyoxylate aminotransferase activity in vitro when associated with T-244 and M-340; causes mitochondrial mistargeting when associated with R-170 and M-340; dbSNP:rs34116584

5hhyA Structure of human alanine:glyoxylate aminotransferase major allele (agt-ma) showing x-ray induced reduction of plp internal aldimine to 4'-deoxy-piridoxine-phosphate (plr) (see paper)
39% identity, 94% coverage: 1:357/378 of query aligns to 21:380/385 of 5hhyA

query
sites
5hhyA

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binding (5-hydroxy-4,6-dimethylpyridin-3-yl)methyl dihydrogen phosphate: S76 (≠ T56), G77 (= G57), H78 (≠ S58), W103 (≠ F83), S153 (≠ T133), D178 (= D159), V180 (= V161), Q203 (= Q184), K204 (= K185), Y255 (= Y233), T258 (= T236)

6rv0A Human alanine:glyoxylate aminotransferase major allele (agt-ma); with pmp in the active site (see paper)
39% identity, 94% coverage: 1:357/378 of query aligns to 21:380/384 of 6rv0A

query
sites
6rv0A

M

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M

L

E

E

A

A

I

L

G

V

N

N

L

V

V

L

E

E

P

P

G

G

D

D

T

S

M

F

L

L

V

V

P

G

T

A

N

N

G

G

Y

I

F
x
W

G

G

G

Q

R

R

M

A

E

V

S

D

M

I

A

G

E

E

R

R

A

I

G

G

G

A

E

R

V

V

V

H

H

P

V

M

D

T

A

K

P

D

W

P

G

G

E

G

P

H

L

Y

D

T

S

L

A

Q

D

E

V

V

Q

E

D

E

A

G

F

L

D

A

E

Q

H

H

Q

K

P

P

D

V

V

L

F

L

G

F

F

L

V

T

H

H

A

G

E

E

T

S

S

S

T

T

G

G

V

V

K

L

Q

Q

P

P

S

-

V

L

P

D

E

G

L

F

T

G

S

E

I

L

A

C

H

H

D

R

H

Y

D

K

A

C

L

L

V

L

A

V

D
|
D

T

S

V
|
V

T
x
A

S

S

L

L

G

G

V

T

E

P

L

L

E

Y

A

M

D

D

D

R

W

Q

G

G

I

I

D

D

V

I

A

L

Y

Y

S

S

G

G

P

S

Q

Q

K
|
K

C

A

L

L

S

N

C

A

P

P

G

G

A

T

S

S

P

L

L

I

T

S

L

F

N

S

D

D

R

K

A

A

M

K

D

K

K

K

V

M

L

Y

S

S

R

R

E

K

E

T

P

K

T

P

R

F

S

S

W

F

Y

Y

L

L

D

D

L

I

S

K

L

W

L

L

E

A

G

N

Y

F

W

W

G

G

D

C

D

D

-

D

-

Q

-

P

R

R

S

M

Y

Y

H

H

T

T

A

I

P

P

I

V

T

I

N

S

V

L

Y

Y

A

S

I

L

R

R

E

E

A

S

L

L

R

A

L

L

V

I

A

A

E

E

E

Q

G

G

I

L

E

E

E

N

R

S

W

W

A

R

R

Q

H

H

R

R

R

E

V

A

A

A

G

A

A

Y

L

L

K

H

A

G

G

R

V

L

E

Q

A

A

M

L

G

G

L

L

G

Q

L

L

N

F

P

V

E

K

D

D

-

P

E

A

Y

L

W

R

L

L

P

P

S

T

L

V

N

T

A

T

V

V

L

A

V

V

P

P

D

A

G

G

V

Y

D

D

A

W

G

R

A

D

A

I

I

V

D

S

Y

Y

V

V

L

I

D

D

H

H

Y

F

D

D

L

I

E

E

I

I

A

M

G

G

L

L

G

G

A

P

L

S

A

T

G

G

D

K

I

V

F

L

R

R

V

I

G

G

C

L

M

L

G

G

H

C

S

N

A

A

R

T

P

R

E

E

N

N

V

V

S

D

F

R

L

V

L

T

T

E

A

A

L

L

G

R

E

A

A

A

L

L

E

Q

binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: S76 (≠ T56), G77 (= G57), H78 (≠ S58), W103 (≠ F83), D178 (= D159), V180 (= V161), A181 (≠ T162), K204 (= K185)

6mfbD Crystal structure of 3-hydroxykynurenine transaminase from aedes aegypti
37% identity, 96% coverage: 1:362/378 of query aligns to 22:386/386 of 6mfbD

query
sites
6mfbD

M

M

G

G

P

P

G

G

P

P

S

S

D

N

V

C

H

S

P

K

R

R

V

V

L

L

R

A

A

A

M

L

S

N

T

N

P

T

L

C

V

L

G

S

H

N

L

F

D

H

P

D

S

E

F

L

I

F

E

Q

V

V

M

I

N

D

E

E

V

V

Q

K

E

D

L

G

M

L

R

R

Y

Y

T

I

F

F

R

Q

T

T

D

E

N

N

K

R

W

T

T

T

I

M

P

C

V

I

S

T

G

G

T
x
S

G
x
A

S
x
H

A

T

S

G

M

M

E

E

A

A

A

L

I

L

G

C

N

N

L

L

V

L

E

E

P

E

G

G

D

D

T

I

M

V

L

L

V

I

P

A

T

N

N

N

G

G

Y

I

F
x
W

G

A

G

E

R

R

M

A

E

I

S

N

M

M

A

A

E

T

R

R

A

Y

G

G

G

A

E

D

V

V

V

R

H

V

V

L

D

E

A

G

P

P

W

A

G

D

E

K

P

P

L

F

D

S

S

M

A

T

D

D

V

F

Q

K

D

K

A

A

F

I

D

E

E

Q

H

H

Q

R

P

P

D

K

V

C

F

L

G

F

F

V

V

V

H

H

A

G

E

D

T
x
S

S

S

T

S

G

G

V

L

K

L

Q

Q

P

P

S

-

V

L

P

E

E

G

L

L

T

G

S

K

I

I

A

C

H

H

D

D

H

Y

D

D

A

C

L

L

V

L

A

V

D
|
D

T

A

V
|
V

T

A

S

S

L

L

G

C

G

G

V

V

E

P

L

F

E

Y

A

M

D

D

D

K

W

W

G

E

I

I

D

D

V

G

A

V

Y

Y

S

T

G

G

P

S

Q
|
Q

K
|
K

C

V

L

L

S

G

C

A

P

P

G

G

A

I

S

T

P

P

L

I

T

S

L

I

N

S

D

P

R

K

A

A

M

L

D

E

K

V

V

I

L

R

S

S

R

R

E

K

E

T

P

P

T

S

R

K

S

V

W

F

Y

Y

L

W

D

D

L

L

S

L

L

I

L

L

E

G

G

N

Y

Y

W

W

G

G

-

C

-

Y

-

D

D

E

D

Q

R

K

S

R

Y
|
Y

H

H

T
|
T

A

V

P

P

I

S

T

N

N

L

V

I

Y

F

A

A

I

L

R

R

E

E

A

A

L

I

R

A

L

Q

V

I

A

A

E

E

G

G

I

L

E

E

E

P

R

V

W

I

A

R

R

R

H

R

R

Q

R

E

V

C

A

A

G

E

A

Q

L

M

K

Y

A

R

G

G

V

L

E

Q

A

A

M

M

G

G

L

L

G

E

L

I

N

F

P

V

E

K

D

D

-

P

E

E

Y

Y

W

R

L

L

P

P

S

T

L

V

N

T

A

C

V

I

L

M

V

I

P

P

D

K

G

G

V

V

D

N

A

W

G

W

A

K

A

V

I

S

D

E

Y

Y

V

A

L

M

D

N

H

N

Y

F

D

S

L

L

E

E

I

I

A

Q

G

G

L

F

G

G

A

P

L

T

A

M

G

G

D

I

I

A

F

W

R

R

V

A

G

G

C

I

M

M

G

G

H

E

S

S

A

S

R

T

P

L

E

Q

N

R

V

V

S

N

F

F

L

Y

L

L

T

Y

A

A

L

F

G

K

E

E

A

S

L

L

E

K

A

A

Q

T

G

H

A

P

D

D

binding pyridoxal-5'-phosphate: S77 (≠ T56), A78 (≠ G57), H79 (≠ S58), W104 (≠ F83), S154 (≠ T133), D179 (= D159), V181 (= V161), Q204 (= Q184), K205 (= K185), Y256 (= Y233), T259 (= T236)

Q0IG34 3-hydroxykynurenine transaminase; 3-hydroxykynurenine transaminase and alanine--glyoxylate aminotransferase; Ae-HKT/AGT; Alanine--glyoxylate aminotransferase; EC 2.6.1.63; EC 2.6.1.44 from Aedes aegypti (Yellowfever mosquito) (Culex aegypti)
37% identity, 96% coverage: 1:362/378 of query aligns to 22:386/400 of Q0IG34

query
sites
Q0IG34

M

M

G

G

P

P

G

G

P

P

S

S

D

N

V

C

H

S

P

K

R

R

V

V

L

L

R

A

A

A

M

L

S

N

T

N

P

T

L

C

V

L

G

S

H

N

L

F

D

H

P

D

S

E

F

L

I

F

E

Q

V

V

M

I

N

D

E

E

V

V

Q

K

E

D

L

G

M

L

R

R

Y

Y

T

I

F

F

R

Q

T

T

D

E

N

N

K

R

W

T

T

T

I

M

P

C

V

I

S

T

G

G

T
x
S

G
x
A

S
x
H

A

T

S

G

M

M

E

E

A

A

A

L

I

L

G

C

N

N

L

L

V

L

E

E

P

E

G

G

D

D

T

I

M

V

L

L

V

I

P

A

T

N

N

N

G

G

Y

I

F

W

G

A

G

E

R

R

M

A

E

I

S

N

M

M

A

A

E

T

R

R

A

Y

G

G

G

A

E

D

V

V

V

R

H

V

V

L

D

G

A

G

P

P

W

A

G

D

E

K

P

P

L

F

D

S

S

M

A

T

D

D

V

F

Q

K

D

K

A

A

F

I

D

E

E

Q

H

H

Q

R

P

P

D

K

V

C

F

L

G

F

F

V

V

V

H

H

A

G

E

D

T
x
S

S

S

T

S

G

G

V

L

K

L

Q

Q

P

P

S

-

V

L

P

E

E

G

L

L

T

G

S

K

I

I

A

C

H

H

D

D

H

Y

D

D

A

C

L

L

V

L

A

V

D

D

T

A

V

V

T

A

S

S

L

L

G

C

G

G

V

V

E

P

L

F

E

Y

A

M

D

D

D

K

W

W

G

E

I

I

D

D

V

G

A

V

Y

Y

S

T

G

G

P

S

Q
|
Q

K
|
K

C

V

L

L

S

G

C

A

P

P

G

G

A

I

S

T

P

P

L

I

T

S

L

I

N

S

D

P

R

K

A

A

M

L

D

E

K

V

V

I

L

R

S

S

R

R

E

K

E

T

P

P

T

S

R

K

S

V

W

F

Y

Y

L

W

D

D

L

L

S

L

L

I

L

L

E

G

G

N

Y

Y

W

W

G

G

-

C

-

Y

-

D

D

E

D

Q

R

K

S

R

Y
|
Y

H

H

T
|
T

A

V

P

P

I

S

T

N

N

L

V

I

Y

F

A

A

I

L

R

R

E

E

A

A

L

I

R

A

L

Q

V

I

A

A

E

E

G

G

I

L

E

E

E

P

R

V

W

I

A

R

R

R

H

R

R

Q

R

E

V

C

A

A

G

E

A

Q

L

M

K

Y

A

R

G

G

V

L

E

Q

A

A

M

M

G

G

L

L

G

E

L

I

N

F

P

V

E

K

D

D

-

P

E

E

Y

Y

W

R

L

L

P

P

S

T

L

V

N

T

A

C

V

I

L

M

V

I

P

P

D

K

G

G

V

V

D

N

A

W

G

W

A

K

A

V

I

S

D

E

Y

Y

V

A

L

M

D

N

H

N

Y

F

D

S

L

L

E

E

I

I

A

Q

G

G

L

F

G

G

A

P

L

T

A

M

G

G

D

I

I

A

F

W

R

R

V

A

G

G

C

I

M

M

G

G

H

E

S

S

A

S

R

T

P

L

E

Q

N

R

V

V

S

N

F

F

L

Y

L

L

T

Y

A

A

L

F

G

K

E

E

A

S

L

L

E

K

A

A

Q

T

G

H

A

P

D

D

SAH 77:79 (≠ TGS 56:58) binding in other chain
S154 (≠ T133) binding in other chain
Q204 (= Q184) binding in other chain
K205 (= K185) modified: N6-(pyridoxal phosphate)lysine
Y256 (= Y233) binding pyridoxal 5'-phosphate
T259 (= T236) binding pyridoxal 5'-phosphate

1h0cA The crystal structure of human alanine:glyoxylate aminotransferase (see paper)
39% identity, 94% coverage: 1:357/378 of query aligns to 23:380/385 of 1h0cA

query
sites
1h0cA

M

L

G

G

P
|
P

G
|
G

P

P

S

S

D

N

V

L

H

P

P

P

R

R

V

I

L

M

R

A

A

A

M

G

S

G

T

L

P

Q

L

M

V

I

G

G

H

S

L

M

D

S

P

K

S

D

F

M

I

Y

E

Q

V

I

M

M

N

D

E

E

V

I

Q

K

E

E

L

G

M

I

R

Q

Y

Y

T

V

F

F

R

Q

T

T

D

R

N

N

K

P

W

L

T

T

I

L

P

V

V

I

S

S

G

G

T
x
S

G
|
G

S
x
H

A

C

S

A

M

L

E

E

A

A

I

L

G

V

N

N

L

V

V

L

E

E

P

P

G

G

D

D

T

S

M

F

L

L

V

V

P

G

T

A

N

N

G

G

Y

I

F
x
W

G

G

G

Q

R

R

M

A

E

V

S

D

M

I

A

G

E

E

R

R

A

I

G

G

G

V

E

H

V

P

V

M

H

T

V

K

D

D

A

P

P

-

W

-

G

G

E

G

P

H

L

Y

D

T

S

L

A

Q

D

E

V

V

Q

E

D

E

A

G

F

L

D

A

E

Q

H

H

Q

K

P

P

D

V

V

L

F

L

G

F

F

L

V

T

H

H

A

G

E

E

T
x
S

S

S

T

T

G

G

V

V

K

L

Q

Q

P

P

S

-

V

L

P

D

E

G

L

F

T

G

S

E

I

L

A

C

H

H

D

R

H

Y

D

K

A

C

L

L

V

L

A

V

D
|
D

T

S

V
|
V

T

A

S

S

L

L

G

G

V

T

E

P

L

L

E

Y

A

M

D

D

D

R

W

Q

G

G

I

I

D

D

V

I

A

L

Y

Y

S

S

G

G

P

S

Q

Q

K
|
K

C

A

L

L

S

N

C

A

P

P

G

G

A

T

S

S

P

L

L

I

T

S

L

F

N

S

D

D

R

K

A

A

M

K

D

K

K

K

V

M

L

Y

S

S

R

R

E

K

E

T

P

K

T

P

R

F

S

S

W

F

Y

Y

L

L

D

D

L

I

S

K

L

W

L

L

E

A

G

N

Y

F

W

W

G

G

D

C

D

D

-

D

-

Q

-

P

R

R

S

M

Y

Y

H

H

T

T

A

I

P

P

I

V

T

I

N

S

V

L

Y

Y

A

S

I

L

R

R

E

E

A

S

L

L

R

A

L

L

V

I

A

A

E

E

E

Q

G

G

I

L

E

E

E

N

R

S

W

W

A

R

R

Q

H

H

R

R

R

E

V

A

A

A

G

A

A

Y

L

L

K

H

A

G

G

R

V

L

E

Q

A

A

M

L

G

G

L

L

G

Q

L

L

N

F

P

V

E

K

D

D

-

P

E

A

Y

L

W

R

L

L

P

P

S

T

L

V

N

T

A

T

V

V

L

A

V

V

P

P

D

A

G

G

V

Y

D

D

A

W

G

R

A

D

A

I

I

V

D

S

Y

Y

V

V

L

I

D

D

H

H

Y

F

D

D

L

I

E

E

I

I

A

M

G

G

L
|
L

G

G

A

P

L

S

A

T

G

G

D

K

I

V

F

L

R
|
R

V

I

G

G

C

L

M

L

G

G

H

C

S

N

A

A

R

T

P

R

E

E

N

N

V

V

S

D

F

R

L

V

L

T

T

E

A

A

L

L

G

R

E

A

A

A

L

L

E

Q

binding (aminooxy)acetic acid: P25 (= P3), G26 (= G4), L346 (= L323), R355 (= R332)
binding pyridoxal-5'-phosphate: S78 (≠ T56), G79 (= G57), H80 (≠ S58), W105 (≠ F83), S153 (≠ T133), D178 (= D159), V180 (= V161), K204 (= K185)

Sites not aligning to the query:

binding pyridoxal-5'-phosphate: 385

Q3LSM4 Alanine--glyoxylate aminotransferase; EC 2.6.1.44 from Aedes aegypti (Yellowfever mosquito) (Culex aegypti) (see paper)
39% identity, 96% coverage: 1:364/378 of query aligns to 23:388/393 of Q3LSM4

query
sites
Q3LSM4

M

M

G

G

P

P

G

G

P

P

S

S

D

N

V

A

H

P

P

Q

R

R

V

V

L

L

R

D

A

A

M

M

S

S

T

R

P

P

L

I

V

L

G

G

H

H

L

L

D

H

P

P

S

E

F

T

I

L

E

K

V

I

M

M

N

D

E

D

V

I

Q

K

E

E

L

G

M

V

R

R

Y

Y

T

L

F

F

R

Q

T

T

D

N

N

N

K

I

W

A

T

T

I

F

P

C

V

L

S

S

G

A

T
x
S

G
|
G

S
x
H

A

G

S

G

M

M

E

E

A

A

A

T

I

L

G

C

N

N

L

L

V

L

E

E

P

D

G

G

D

D

T

V

M

I

L

L

V

I

P

G

T

H

N

T

G

G

Y

H

F

W

G

G

G

D

R

R

M

S

E

A

S

D

M

M

A

A

E

T

R

R

A

Y

G

G

G

A

E

D

V

V

V

R

H

V

V

V

D

K

A

S

P

K

W

V

G

G

E

Q

P

S

L

L

D

S

S

L

A

D

D

E

V

I

Q

R

D

D

A

A

F

L

D

L

E

I

H

H

Q

K

P

P

D

S

V

V

F

L

G

F

F

L

V

T

H

Q

A

G

E

D

T
x
S

S

S

T

T

G

G

V

V

K

L

Q

Q

P

-

S

G

V

L

P

E

E

G

L

V

T

G

S

A

I

L

A

C

H

H

D

Q

H

H

D

N

A

C

L

L

V

L

V

I

A

V

D

D

T

T

V

V

T

A

S

S

L

L

G

G

V

A

E

P

L

M

E

F

A

M

D

D

D

R

W

W

G

E

I

I

D

D

V

A

A

M

Y

Y

S

T

G

G

P

S

Q
|
Q

K
|
K

C

V

L

L

S

G

C

A

P

P

G

G

A

I

S

T

P

P

L

V

T

S

L

F

N

S

D

H

R

R

A

A

M

V

D

E

K

R

V

Y

L

K

S

R

R

R

E

N

E

T

P

K

T

V

R

K

S

V

W

Y

Y

Y

L

W

D

D

L

M

S

S

L

L

L

V

E

G

G

D

Y

Y

W

W

-

G

-

C

-

F

G

G

D

R

D

P

R

R

S

I

Y
|
Y

H

H

T
|
T

A

I

P

S

I

S

T

T

N

L

V

L

Y

Y

A

G

I

L

R

R

E

E

A

A

L

I

R

A

L

M

V

A

A

C

E

E

G

G

I

L

E

P

E

A

R

L

W

I

A

A

R

R

H

H

R

E

R

D

V

C

A

A

G

K

A

R

L

L

K

Y

A

R

G

G

V

L

E

Q

A

D

M

A

G

G

L

F

G

E

L

L

N

Y

P

A

E

D

D

P

E

K

Y

D

W

R

L

L

P

S

S

T

L

V

N

T

A

T

V

I

L

K

V

V

P

P

D

Q

G

G

V

V

D

D

A

W

G

L

A

K

A

A

I

A

D

Q

Y

Y

V

A

L

M

D

K

H

T

Y

Y

D

L

L

V

E

E

I

I

A

S

G

G

L

L

G

G

A

P

L

T

A

A

G

G

D

Q

I

V

F

F

R
|
R

V

I

G

G

C

L

M

M

G

G

H

Q

S

N

A

A

R

T

P

T

E

E

N

R

V

V

S

D

F

R

L

V

L

L

T

Q

A

V

L

F

G

Q

E

E

A

A

L

V

E

A

A

A

Q

V

G

K

A

P

D

D

V

V

D

Q

SGH 78:80 (≠ TGS 56:58) binding in other chain
S155 (≠ T133) binding glyoxylate; binding L-alanine
Q205 (= Q184) binding in other chain
K206 (= K185) modified: N6-(pyridoxal phosphate)lysine
Y257 (= Y233) binding pyridoxal 5'-phosphate
T260 (= T236) binding pyridoxal 5'-phosphate
R356 (= R332) binding glyoxylate

Q7PRG3 3-hydroxykynurenine transaminase; AgHKT; Alanine--glyoxylate aminotransferase; EC 2.6.1.63; EC 2.6.1.44 from Anopheles gambiae (African malaria mosquito) (see paper)
37% identity, 96% coverage: 1:362/378 of query aligns to 22:386/396 of Q7PRG3

query
sites
Q7PRG3

M

M

G

G

P

P

G

G

P

P

S

S

D

N

V

C

H

S

P

K

R

R

V

V

L

L

R

T

A

A

M

M

S

T

T

N

P

T

L

V

V

L

G

S

H

N

L

F

D

H

P

A

S

E

F

L

I

F

E

R

V

T

M

M

N

D

E

E

V

V

Q

K

E

D

L

G

M

L

R

R

Y

Y

T

I

F

F

R

Q

T

T

D

E

N

N

K

R

W

A

T

T

I

M

P

C

V

V

S

S

G

G

T
x
S

G
x
A

S
x
H

A

A

S

G

M

M

E

E

A

A

A

M

I

L

G

S

N

N

L

L

V

L

E

E

P

E

G

G

D

D

T

R

M

V

L

L

V

I

P

A

T

V

N

N

G

G

Y

I

F

W

G

A

G

E

R

R

M

A

E

V

S

E

M

M

A

S

E

E

R

R

A

Y

G

G

G

A

E

D

V

V

V

R

H

T

V

I

D

E

A

G

P

P

W

P

G

D

E

R

P

P

L

F

D

S

S

L

A

E

D

T

V

L

Q

A

D

R

A

A

F

I

D

E

E

L

H

H

Q

Q

P

P

D

K

V

C

F

L

G

F

F

L

V

T

H

H

A

G

E

D

T
x
S

S

S

T

S

G

G

V

L

K

L

Q

Q

P

P

S

-

V

L

P

E

E

G

L

V

T

G

S

Q

I

I

A

C

H

H

D

Q

H

H

D

D

A

C

L

L

V

L

V

I

A

V

D

D

T

A

V

V

T

A

S

S

L

L

G

C

G

G

V

V

E

P

L

F

E

Y

A

M

D

D

D

K

W

W

G

E

I

I

D

D

V

A

A

V

Y

Y

S

T

G

G

P

A

Q
|
Q

K
|
K

C

V

L

L

S

G

C

A

P

P

G

G

A

I

S

T

P

P

L

I

T

S

L

I

N

S

D

P

R

K

A

A

M

L

D

D

K

V

V

I

L

R

S

N

R

R

E

R

E

T

P

K

T

S

R

K

S

V

W

F

Y

Y

L

W

D

D

L

L

S

L

L

L

E

G

G

N

Y

Y

W

W

G

G

-

C

-

Y

-

D

D

E

D

P

R

K

S

R

Y
|
Y

H

H

T
|
T

A

V

P

A

I

S

T

N

N

L

V

I

Y

F

A

A

I

L

R

R

E

E

A

A

L

L

R

A

L

Q

V

I

A

A

E

E

G

G

I

L

E

E

E

N

R

Q

W

I

A

K

R

R

H

R

R

I

R

E

V

C

A

A

G

Q

A

I

L

L

K

Y

A

E

G

G

V

L

E

G

A

K

M

M

G

G

L

L

G

D

L

I

N

F

P

V

E

K

D

D

-

P

E

R

Y

H

W

R

L

L

P

P

S

T

L

V

N

T

A

G

V

I

L

M

V

I

P

P

D

K

G

G

V

V

D

D

A

W

G

W

A

K

A

V

I

S

D

Q

Y

Y

V

A

L

M

D

N

H

N

Y

F

D

S

L

L

E

E

I

V

A

Q

G

G

L

L

G

G

A

P

L

T

A

F

G

G

D

K

I

A

F

W

R

R

V

V

G

G

C

I

M

M

G

G

H

E

S

C

A

S

R

T

P

V

E

Q

N

K

V

I

S

Q

F

F

L

Y

L

L

T

Y

A

G

L

F

G

K

E

E

A

S

L

L

E

K

A

A

Q

T

G

H

A

P

D

D

SAH 77:79 (≠ TGS 56:58) binding in other chain
S154 (≠ T133) binding in other chain
Q204 (= Q184) binding in other chain
K205 (= K185) modified: N6-(pyridoxal phosphate)lysine
Y256 (= Y233) binding pyridoxal 5'-phosphate
T259 (= T236) binding pyridoxal 5'-phosphate

2huuA Crystal structure of aedes aegypti alanine glyoxylate aminotransferase in complex with alanine (see paper)
39% identity, 95% coverage: 1:358/378 of query aligns to 23:382/385 of 2huuA

query
sites
2huuA

M

M

G

G

P
|
P

G

G

P

P

S

S

D

N

V

A

H

P

P

Q

R

R

V

V

L

L

R

D

A

A

M

M

S

S

T

R

P

P

L

I

V

L

G

G

H
|
H

L

L

D

H

P

P

S

E

F

T

I

L

E

K

V

I

M

M

N

D

E

D

V

I

Q

K

E

E

L

G

M

V

R

R

Y

Y

T

L

F

F

R

Q

T

T

D

N

N

N

K

I

W

A

T

T

I

F

P

C

V

L

S

S

G

A

T

S

G

G

S

H

A

G

S

G

M

M

E

E

A

A

A

T

I

L

G

C

N

N

L

L

V

L

E

E

P

D

G

G

D

D

T

V

M

I

L

L

V

I

P

G

T

H

N

T

G

G

Y

H

F

W

G

G

G

D

R

R

M

S

E

A

S

D

M

M

A

A

E

T

R

R

A

Y

G

G

G

A

E

D

V

V

V

R

H

V

V

V

D

K

A

S

P

K

W

V

G

G

E

Q

P

S

L

L

D

S

S

L

A

D

D

E

V

I

Q

R

D

D

A

A

F

L

D

L

E

I

H

H

Q

K

P

P

D

S

V

V

F

L

G

F

F

L

V

T

H

Q

A

G

E

D

T
x
S

S

S

T

T

G

G

V

V

K

L

Q

Q

P

-

S

G

V

L

P

E

E

G

L

V

T

G

S

A

I

L

A

C

H

H

D

Q

H

H

D

N

A

C

L

L

V

L

V

I

A

V

D

D

T

T

V

V

T

A

S

S

L

L

G

G

V

A

E

P

L

M

E

F

A

M

D

D

D

R

W

W

G

E

I

I

D

D

V

A

A

M

Y

Y

S

T

G

G

P

S

Q

Q

K
|
K

C

V

L

L

S

G

C

A

P

P

G

G

A

I

S

T

P

P

L

V

T

S

L

F

N

S

D

H

R

R

A

A

M

V

D

E

K

R

V

Y

L

K

S

R

R

R

E

N

E

T

P

K

T

V

R

K

S

V

W

Y

Y

Y

L

W

D

D

L

M

S

S

L

L

L

V

E

G

G

D

Y

Y

W

W

-

G

-

C

-

F

G

G

D

R

D

P

R

R

S

I

Y
|
Y

H

H

T

T

A

I

P

S

I

S

T

T

N

L

V

L

Y

Y

A

G

I

L

R

R

E

E

A

A

L

I

R

A

L

M

V

A

A

C

E

E

G

G

I

L

E

P

E

A

R

L

W

I

A

A

R

R

H

H

R

E

R

D

V

C

A

A

G

K

A

R

L

L

K

Y

A

R

G

G

V

L

E

Q

A

D

M

A

G

G

L

F

G

E

L

L

N

Y

P

A

E

D

D

P

E

K

Y

D

W

R

L

L

P

S

S

T

L

V

N

T

A

T

V

I

L

K

V

V

P

P

D

Q

G

G

V

V

D

D

A

W

G

L

A

K

A

A

I

A

D

Q

Y

Y

V

A

L

M

D

K

H

T

Y

Y

D

L

L

V

E

E

I

I

A

S

G

G

L
|
L

G

G

A

P

L

T

A

A

G

G

D

Q

I

V

F

F

R
|
R

V

I

G

G

C

L

M

M

G

G

H

Q

S

N

A

A

R

T

P

T

E

E

N

R

V

V

S

D

F

R

L

V

L

L

T

Q

A

V

L

F

G

Q

E

E

A

A

L

V

E

A

A

A

binding 1-butanol: H45 (= H23), Y257 (= Y233)
binding alanine: P25 (= P3), S155 (≠ T133), K206 (= K185), L347 (= L323), R356 (= R332)

2huiA Crystal structure of aedes aegypti alanine glyoxylate aminotransferase in complex with glyoxylic acid (see paper)
39% identity, 95% coverage: 1:358/378 of query aligns to 23:382/385 of 2huiA

query
sites
2huiA

M

M

G

G

P
|
P

G

G

P

P

S

S

D

N

V

A

H

P

P

Q

R

R

V

V

L

L

R

D

A

A

M

M

S

S

T

R

P

P

L

I

V

L

G

G

H

H

L

L

D

H

P

P

S

E

F

T

I

L

E

K

V

I

M

M

N

D

E

D

V

I

Q

K

E

E

L

G

M

V

R

R

Y

Y

T

L

F

F

R

Q

T

T

D

N

N

N

K

I

W

A

T

T

I

F

P

C

V

L

S

S

G

A

T

S

G

G

S

H

A

G

S

G

M

M

E

E

A

A

A

T

I

L

G

C

N

N

L

L

V

L

E

E

P

D

G

G

D

D

T

V

M

I

L

L

V

I

P

G

T

H

N

T

G

G

Y

H

F

W

G

G

G

D

R

R

M

S

E

A

S

D

M

M

A

A

E

T

R

R

A

Y

G

G

G

A

E

D

V

V

V

R

H

V

V

V

D

K

A

S

P

K

W

V

G

G

E

Q

P

S

L

L

D

S

S

L

A

D

D

E

V

I

Q

R

D

D

A

A

F

L

D

L

E

I

H

H

Q

K

P

P

D

S

V

V

F

L

G

F

F

L

V

T

H

Q

A

G

E

D

T
x
S

S

S

T

T

G

G

V

V

K

L

Q

Q

P

-

S

G

V

L

P

E

E

G

L

V

T

G

S

A

I

L

A

C

H

H

D

Q

H

H

D

N

A

C

L

L

V

L

V

I

A

V

D

D

T

T

V

V

T

A

S

S

L

L

G

G

V

A

E

P

L

M

E

F

A

M

D

D

D

R

W

W

G

E

I

I

D

D

V

A

A

M

Y

Y

S

T

G

G

P

S

Q

Q

K
|
K

C

V

L

L

S

G

C

A

P

P

G

G

A

I

S

T

P

P

L

V

T

S

L

F

N

S

D

H

R

R

A

A

M

V

D

E

K

R

V

Y

L

K

S

R

R

R

E

N

E

T

P

K

T

V

R

K

S

V

W

Y

Y

Y

L

W

D

D

L

M

S

S

L

L

L

V

E

G

G

D

Y

Y

W

W

-

G

-

C

-

F

G

G

D

R

D

P

R

R

S

I

Y

Y

H

H

T

T

A

I

P

S

I

S

T

T

N

L

V

L

Y

Y

A

G

I

L

R

R

E

E

A

A

L

I

R

A

L

M

V

A

A

C

E

E

G

G

I

L

E

P

E

A

R

L

W

I

A

A

R

R

H

H

R

E

R

D

V

C

A

A

G

K

A

R

L

L

K

Y

A

R

G

G

V

L

E

Q

A

D

M

A

G

G

L

F

G

E

L

L

N

Y

P

A

E

D

D

P

E

K

Y

D

W

R

L

L

P

S

S

T

L

V

N

T

A

T

V

I

L

K

V

V

P

P

D

Q

G

G

V

V

D

D

A

W

G

L

A

K

A

A

I

A

D

Q

Y

Y

V

A

L

M

D

K

H

T

Y

Y

D

L

L

V

E

E

I

I

A

S

G

G

L

L

G

G

A

P

L

T

A

A

G

G

D

Q

I

V

F

F

R
|
R

V

I

G

G

C

L

M

M

G

G

H

Q

S

N

A

A

R

T

P

T

E

E

N

R

V

V

S

D

F

R

L

V

L

L

T

Q

A

V

L

F

G

Q

E

E

A

A

L

V

E

A

A

A

binding glyoxylic acid: P25 (= P3), S155 (≠ T133), K206 (= K185), R356 (= R332)

2hufA Crystal structure of aedes aegypti alanine glyoxylate aminotransferase (see paper)
39% identity, 95% coverage: 1:358/378 of query aligns to 23:382/385 of 2hufA

query
sites
2hufA

M

M

G

G

P

P

G

G

P

P

S

S

D

N

V

A

H

P

P

Q

R

R

V

V

L

L

R

D

A

A

M

M

S

S

T

R

P

P

L

I

V

L

G

G

H
|
H

L

L

D

H

P

P

S

E

F

T

I

L

E

K

V

I

M

M

N

D

E

D

V

I

Q

K

E

E

L

G

M

V

R

R

Y

Y

T

L

F

F

R

Q

T

T

D

N

N

N

K

I

W

A

T

T

I

F

P

C

V

L

S

S

G

A

T

S

G

G

S

H

A

G

S

G

M

M

E

E

A

A

A

T

I

L

G

C

N

N

L

L

V

L

E

E

P

D

G

G

D

D

T

V

M

I

L

L

V

I

P

G

T

H

N

T

G

G

Y

H

F

W

G

G

G

D

R

R

M

S

E

A

S

D

M

M

A

A

E

T

R

R

A

Y

G

G

G

A

E

D

V

V

V

R

H

V

V

V

D

K

A

S

P

K

W

V

G

G

E

Q

P

S

L

L

D

S

S

L

A

D

D

E

V

I

Q

R

D

D

A

A

F

L

D

L

E

I

H

H

Q

K

P

P

D

S

V

V

F

L

G

F

F

L

V

T

H

Q

A

G

E

D

T
x
S

S

S

T

T

G

G

V

V

K

L

Q

Q

P

-

S

G

V

L

P

E

E

G

L

V

T

G

S

A

I

L

A

C

H

H

D

Q

H

H

D

N

A

C

L

L

V

L

V

I

A

V

D

D

T

T

V

V

T

A

S

S

L

L

G

G

V

A

E

P

L

M

E

F

A

M

D

D

D

R

W

W

G

E

I

I

D

D

V

A

A

M

Y

Y

S

T

G

G

P

S

Q

Q

K

K

C

V

L

L

S

G

C

A

P

P

G

G

A

I

S

T

P

P

L

V

T

S

L

F

N

S

D

H

R

R

A

A

M

V

D

E

K

R

V

Y

L

K

S

R

R

R

E

N

E

T

P

K

T

V

R

K

S

V

W

Y

Y

Y

L

W

D

D

L

M

S

S

L

L

L

V

E

G

G

D

Y

Y

W

W

-

G

-

C

-

F

G

G

D

R

D

P

R

R

S

I

Y
|
Y

H

H

T

T

A

I

P

S

I

S

T

T

N

L

V

L

Y

Y

A

G

I

L

R

R

E

E

A

A

L

I

R

A

L

M

V

A

A

C

E

E

G

G

I

L

E

P

E

A

R

L

W

I

A

A

R

R

H

H

R

E

R

D

V

C

A

A

G

K

A

R

L

L

K

Y

A

R

G

G

V

L

E

Q

A

D

M

A

G

G

L

F

G

E

L

L

N

Y

P

A

E

D

D

P

E

K

Y

D

W

R

L

L

P

S

S

T

L

V

N

T

A

T

V

I

L

K

V

V

P

P

D

Q

G

G

V

V

D

D

A

W

G

L

A

K

A

A

I

A

D

Q

Y

Y

V

A

L

M

D

K

H

T

Y

Y

D

L

L

V

E

E

I

I

A

S

G

G

L

L

G

G

A

P

L

T

A

A

G

G

D

Q

I

V

F

F

R
|
R

V

I

G

G

C

L

M

M

G

G

H

Q

S

N

A

A

R

T

P

T

E

E

N

R

V

V

S

D

F

R

L

V

L

L

T

Q

A

V

L

F

G

Q

E

E

A

A

L

V

E

A

A

A

binding 1-butanol: H45 (= H23), S155 (≠ T133), Y257 (= Y233), R356 (= R332)

2ch2A Structure of the anopheles gambiae 3-hydroxykynurenine transaminase in complex with inhibitor (see paper)
37% identity, 96% coverage: 1:362/378 of query aligns to 20:384/387 of 2ch2A

query
sites
2ch2A

M

M

G

G

P

P

G
|
G

P

P

S

S

D

N

V

C

H

S

P

K

R

R

V

V

L

L

R

T

A

A

M

M

S

T

T

N

P

T

L

V

V

L

G
x
S

H
x
N

L

F

D

H

P

A

S

E

F

L

I

F

E

R

V

T

M

M

N

D

E

E

V

V

Q

K

E

D

L

G

M

L

R

R

Y

Y

T

I

F

F

R

Q

T

T

D

E

N

N

K

R

W

A

T

T

I

M

P

C

V

V

S

S

G

G

T
x
S

G
x
A

S
x
H

A

A

S

G

M

M

E

E

A

A

A

M

I

L

G

S

N

N

L

L

V

L

E

E

P

E

G

G

D

D

T

R

M

V

L

L

V

I

P

A

T

V

N

N

G

G

Y

I

F
x
W

G

A

G

E

R

R

M

A

E

V

S

E

M

M

A

S

E

E

R

R

A

Y

G

G

G

A

E

D

V

V

V

R

H

T

V

I

D

E

A

G

P

P

W

P

G

D

E

R

P

P

L

F

D

S

S

L

A

E

D

T

V

L

Q

A

D

R

A

A

F

I

D

E

E

L

H

H

Q

Q

P

P

D

K

V

C

F

L

G

F

F

L

V

T

H

H

A

G

E

D

T
x
S

S

S

T

S

G

G

V

L

K

L

Q

Q

P

P

S

-

V

L

P

E

E

G

L

V

T

G

S

Q

I

I

A

C

H

H

D

Q

H

H

D

D

A

C

L

L

V

L

V

I

A

V

D
|
D

T

A

V
|
V

T

A

S

S

L

L

G

C

G

G

V

V

E

P

L

F

E

Y

A

M

D

D

D

K

W

W

G

E

I

I

D

D

V

A

A

V

Y

Y

S

T

G

G

P

A

Q

Q

K
|
K

C

V

L

L

S

G

C

A

P

P

G

G

A

I

S

T

P

P

L

I

T

S

L

I

N

S

D

P

R

K

A

A

M

L

D

D

K

V

V

I

L

R

S

N

R

R

E

R

E

T

P

K

T

S

R

K

S

V

W

F

Y

Y

L

W

D

D

L

L

S

L

L

L

E

G

G

N

Y

Y

W

W

G

G

-

C

-

Y

-

D

D

E

D

P

R

K

S

R

Y
|
Y

H

H

T
|
T

A

V

P

A

I

S

T

N

N

L

V

I

Y

F

A

A

I

L

R

R

E

E

A

A

L

L

R

A

L

Q

V

I

A

A

E

E

G

G

I

L

E

E

E

N

R

Q

W

I

A

K

R

R

H

R

R

I

R

E

V

C

A

A

G

Q

A

I

L

L

K

Y

A

E

G

G

V

L

E

G

A

K

M

M

G

G

L

L

G

D

L

I

N

F

P

V

E

K

D

D

-

P

E

R

Y

H

W

R

L

L

P

P

S

T

L

V

N

T

A

G

V

I

L

M

V

I

P

P

D

K

G

G

V

V

D

D

A

W

G

W

A

K

A

V

I

S

D

Q

Y

Y

V

A

L

M

D

N

H

N

Y

F

D

S

L

L

E

E

I

V

A
x
Q

G

G

L
|
L

G

G

A

P

L

T

A

F

G

G

D

K

I

A

F

W

R
|
R

V

V

G

G

C

I

M

M

G

G

H

E

S

C

A

S

R

T

P

V

E

Q

N

K

V

I

S

Q

F

F

L

Y

L

L

T

Y

A

G

L

F

G

K

E

E

A

S

L

L

E

K

A

A

Q

T

G

H

A

P

D

D

binding 4-(2-aminophenyl)-4-oxobutanoic acid: G23 (= G4), S41 (≠ G22), N42 (≠ H23), S152 (≠ T133), Y254 (= Y233), Q342 (≠ A320), L345 (= L323), R354 (= R332)
binding pyridoxal-5'-phosphate: S75 (≠ T56), A76 (≠ G57), H77 (≠ S58), W102 (≠ F83), S152 (≠ T133), D177 (= D159), V179 (= V161), K203 (= K185), Y254 (= Y233), T257 (= T236)

2ch1A Structure of anopheles gambiae 3-hydroxykynurenine transaminase (see paper)
37% identity, 96% coverage: 1:362/378 of query aligns to 21:385/388 of 2ch1A

query
sites
2ch1A

M

M

G

G

P

P

G

G

P

P

S

S

D

N

V

C

H

S

P

K

R

R

V

V

L

L

R

T

A

A

M

M

S

T

T

N

P

T

L

V

V

L

G

S

H

N

L

F

D

H

P

A

S

E

F

L

I

F

E

R

V

T

M

M

N

D

E

E

V

V

Q

K

E

D

L

G

M

L

R

R

Y

Y

T

I

F

F

R

Q

T

T

D

E

N

N

K

R

W

A

T

T

I

M

P

C

V

V

S

S

G

G

T
x
S

G
x
A

S
x
H

A

A

S

G

M

M

E

E

A

A

A

M

I

L

G

S

N

N

L

L

V

L

E

E

P

E

G

G

D

D

T

R

M

V

L

L

V

I

P

A

T

V

N

N

G

G

Y

I

F
x
W

G

A

G

E

R

R

M

A

E

V

S

E

M

M

A

S

E

E

R

R

A

Y

G

G

G

A

E

D

V

V

V

R

H

T

V

I

D

E

A

G

P

P

W

P

G

D

E

R

P

P

L

F

D

S

S

L

A

E

D

T

V

L

Q

A

D

R

A

A

F

I

D

E

E

L

H

H

Q

Q

P

P

D

K

V

C

F

L

G

F

F

L

V

T

H

H

A

G

E

D

T
x
S

S

S

T

S

G

G

V

L

K

L

Q

Q

P

P

S

-

V

L

P

E

E

G

L

V

T

G

S

Q

I

I

A

C

H

H

D

Q

H

H

D

D

A

C

L

L

V

L

V

I

A

V

D
|
D

T

A

V
|
V

T

A

S

S

L

L

G

C

G

G

V

V

E

P

L

F

E

Y

A

M

D

D

D

K

W

W

G

E

I

I

D

D

V

A

A

V

Y

Y

S

T

G

G

P

A

Q
|
Q

K
|
K

C

V

L

L

S

G

C

A

P

P

G

G

A

I

S

T

P

P

L

I

T

S

L

I

N

S

D

P

R

K

A

A

M

L

D

D

K

V

V

I

L

R

S

N

R

R

E

R

E

T

P

K

T

S

R

K

S

V

W

F

Y

Y

L

W

D

D

L

L

S

L

L

L

E

G

G

N

Y

Y

W

W

G

G

-

C

-

Y

-

D

D

E

D

P

R

K

S

R

Y
|
Y

H

H

T
|
T

A

V

P

A

I

S

T

N

N

L

V

I

Y

F

A

A

I

L

R

R

E

E

A

A

L

L

R

A

L

Q

V

I

A

A

E

E

G

G

I

L

E

E

E

N

R

Q

W

I

A

K

R

R

H

R

R

I

R

E

V

C

A

A

G

Q

A

I

L

L

K

Y

A

E

G

G

V

L

E

G

A

K

M

M

G

G

L

L

G

D

L

I

N

F

P

V

E

K

D

D

-

P

E

R

Y

H

W

R

L

L

P

P

S

T

L

V

N

T

A

G

V

I

L

M

V

I

P

P

D

K

G

G

V

V

D

D

A

W

G

W

A

K

A

V

I

S

D

Q

Y

Y

V

A

L

M

D

N

H

N

Y

F

D

S

L

L

E

E

I

V

A

Q

G

G

L

L

G

G

A

P

L

T

A

F

G

G

D

K

I

A

F

W

R

R

V

V

G

G

C

I

M

M

G

G

H

E

S

C

A

S

R

T

P

V

E

Q

N

K

V

I

S

Q

F

F

L

Y

L

L

T

Y

A

G

L

F

G

K

E

E

A

S

L

L

E

K

A

A

Q

T

G

H

A

P

D

D

binding pyridoxal-5'-phosphate: S76 (≠ T56), A77 (≠ G57), H78 (≠ S58), W103 (≠ F83), S153 (≠ T133), D178 (= D159), V180 (= V161), Q203 (= Q184), K204 (= K185), Y255 (= Y233), T258 (= T236)

2yrrA Hypothetical alanine aminotransferase (tth0173) from thermus thermophilus hb8
36% identity, 94% coverage: 1:356/378 of query aligns to 4:350/352 of 2yrrA

query
sites
2yrrA

M

L

G

T

P

P

G

G

P

P

S

T

D

P

V

I

H

P

P

E

R

R

V

V

L

Q

R

K

A

A

M

L

S

L

T

R

P

P

L

M

V

R

G

G

H

H

L

L

D

D

P

P

S

E

F

V

I

L

E

R

V

V

M

N

N

R

E

A

V

I

Q

Q

E

E

L

R

M

L

R

A

Y

A

T

L

F

F

R

D

T

P

-

G

D

E

N

G

K

A

W

L

T

V

I

A

P

A

V

L

S

A

G

G

T
x
S

G
|
G

S
|
S

A

L

S

G

M

M

E

E

A

A

A

G

I

L

G

A

N

N

L

L

V

D

E

R

P

-

G

-

D

G

T

P

M

V

L

L

V

V

P

L

T

V

N

N

G

G

Y

A

F
|
F

G

S

G

Q

R

R

M

V

E

A

S

E

M

M

A

A

E

A

R

L

A

H

G

G

G

L

E

D

V

P

V

E

H

V

V

L

D

D

A

F

P

P

W

P

G

G

E

E

P

P

L

V

D

D

S

P

A

E

D

A

V

V

Q

A

D

R

A

A

F

L

D

K

E

R

H

R

Q

R

P

Y

D

R

V

M

F

V

G

A

F

L

V

V

H

H

A

G

E

E

T
|
T

S

S

T

T

G

G

V

V

K

L

Q

N

P

P

S

A

V

-

P

E

E

A

L

I

T

G

S

A

I

L

A

A

H

K

D

E

H

A

D

G

A

A

L

L

V

F

A

L

D
|
D

T

A

V
|
V

T

T

S

T

L

L

G

G

G

M

V

L

E

P

L

F

E

S

A

M

D

R

D

A

W

M

G

G

I

V

D

D

V

Y

A

A

Y

F

S

T

G

G

P

S

Q

Q

K
|
K

C

C

L

L

S

S

C

A

P

P

G

G

A

L

S

A

P

P

L

I

T

A

L

A

N

S

D

L

R

E

A

A

M

R

D

K

K

A

V

F

L

T

S

G

R

K

E

-

P

-

T

-

R

R

S

G

W

W

Y

Y

L

L

D

D

L

L

S

A

L

R

L

V

E

A

G

E

Y

H

W

W

G

-

D

E

D

R

R

G

S

G

Y

Y

H

H

T

T

A

T

P

P

I

V

T

L

N

L

V

H

Y

Y

A

A

I

L

R

L

E

E

A

A

L

L

R

D

L

L

V

V

A

L

E

E

G

G

I

V

E

A

R

R

W

E

A

R

R

R

H

A

R

R

R

E

V

V

A

Y

G

A

A

W

L

V

K

L

A

E

G

E

V

L

E

K

A

A

M

R

G

G

L

F

G

R

L

P

N

Y

P

P

E

K

D

A

E

S

Y

P

W

-

L

L

P

P

S

T

L

V

N

L

A

V

V

V

L

R

V

P

P

P

D

E

G

G

V

V

D

D

A

A

G

D

A

R

A

L

I

V

D

R

Y

-

V

A

L

L

D

Y

H

A

Y

E

D

G

L

V

E

A

I

V

A

A

G

G

L

I

G

G

A

P

L

T

A

R

G

G

D

Q

I

V

F

L

R

R

V

L

G

G

C

L

M

M

G

G

H

E

S

G

A

A

R

R

P

R

E

E

N

A

V

Y

S

Q

F

A

L

F

L

L

T

K

A

A

L

L

G

D

E

R

A

A

L

L

binding pyridoxal-5'-phosphate: S60 (≠ T56), G61 (= G57), S62 (= S58), F85 (= F83), T135 (= T133), D160 (= D159), V162 (= V161), K186 (= K185)

Query Sequence

>WP_004042827.1 NCBI__GCF_000337315.1:WP_004042827.1
MGPGPSDVHPRVLRAMSTPLVGHLDPSFIEVMNEVQELMRYTFRTDNKWTIPVSGTGSAS
MEAAIGNLVEPGDTMLVPTNGYFGGRMESMAERAGGEVVHVDAPWGEPLDSADVQDAFDE
HQPDVFGFVHAETSTGVKQPSVPELTSIAHDHDALVVADTVTSLGGVELEADDWGIDVAY
SGPQKCLSCPPGASPLTLNDRAMDKVLSREEPTRSWYLDLSLLEGYWGDDRSYHHTAPIT
NVYAIREALRLVAEEGIEERWARHRRVAGALKAGVEAMGLGLNPEDEYWLPSLNAVLVPD
GVDAGAAIDYVLDHYDLEIAGGLGALAGDIFRVGCMGHSARPENVSFLLTALGEALEAQG
ADVDPEAGFAAMREALNQ

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory