SitesBLAST

P09114 Acetolactate synthase 2, chloroplastic; ALS II; Acetohydroxy-acid synthase II; Acetolactate synthase II; EC 2.2.1.6 from Nicotiana tabacum (Common tobacco) (see paper)
44% identity, 94% coverage: 24:576/587 of query aligns to 87:650/664 of P09114

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P191 (= P127) mutation to A: In S4-Hra; highly resistant to sulfonylurea herbicides; when associated with L-568.
W568 (= W501) mutation to L: In S4-Hra; highly resistant to sulfonylurea herbicides; when associated with A-191.

8et4A Crystal structure of wild-type arabidopsis thaliana acetohydroxyacid synthase in complex with amidosulfuron (see paper)
43% identity, 94% coverage: 24:575/587 of query aligns to 8:570/582 of 8et4A

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binding 2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-[(1~{S})-1-(dioxidanyl)-1-oxidanyl-ethyl]-4-methyl-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: V400 (= V411), G401 (= G412), Q402 (= Q413), H403 (= H414), G426 (= G437), M428 (= M439), G452 (= G464), D453 (= D465), G454 (≠ A466), S455 (= S467), M458 (= M470), N480 (= N492), H482 (≠ Y494), L483 (≠ I495), G484 (= G496), M485 (= M497), V486 (= V498)
binding flavin-adenine dinucleotide: R161 (= R176), G222 (= G235), G223 (= G236), G224 (= G237), T246 (= T261), L247 (≠ M262), M248 (≠ P263), L264 (≠ A279), M266 (= M281), H267 (= H282), G286 (= G301), V287 (≠ T302), R288 (= R303), D290 (= D305), R292 (= R307), V293 (≠ L308), D310 (= D325), I311 (= I326), D329 (= D344), V330 (≠ A345), M405 (= M416), G423 (≠ H434)
binding magnesium ion: F370 (≠ Y380), D453 (= D465), M458 (= M470), Q461 (= Q473), N480 (= N492), H482 (≠ Y494), K533 (≠ Y538)
binding N-{[(4,6-dimethoxypyrimidin-2-yl)carbamoyl]sulfamoyl}-N-methylmethanesulfonamide: M266 (= M281), R292 (= R307), M485 (= M497), W489 (= W501), S568 (= S573)

5wj1A Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a triazolopyrimidine herbicide, penoxsulam (see paper)
43% identity, 94% coverage: 24:575/587 of query aligns to 8:570/582 of 5wj1A

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A

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E

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R

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K

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M

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I
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I

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-

L

-

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-

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-

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x
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A

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-

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Q

E

E

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L

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A

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A

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R

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E

E

N

N

L

L

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I

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V

V

A

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I

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A

-

H

-

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-

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-

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A

A

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E

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D

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P

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N

F

M

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K

L

L

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A

A

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A

A

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C

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G

A

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A

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A

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Y

K

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A

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I

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E

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M

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L

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Y

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D

P

G

G

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Y

V

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L

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D

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I

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H

A

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H

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L

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S
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S

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G

active site: Y33 (≠ V49), G35 (= G51), G36 (= G52), A37 (= A53), S38 (≠ I54), E59 (= E74), T82 (= T97), F121 (= F136), Q122 (= Q137), E123 (= E138), K171 (= K186), M266 (= M281), V293 (≠ L308), V400 (= V411), G426 (= G437), M428 (= M439), D453 (= D465), N480 (= N492), H482 (≠ Y494), L483 (≠ I495), M485 (= M497), V486 (= V498), W489 (= W501), H558 (≠ P563)
binding flavin-adenine dinucleotide: R161 (= R176), G222 (= G235), G223 (= G236), G224 (= G237), T246 (= T261), L247 (≠ M262), M248 (≠ P263), M263 (≠ W278), L264 (≠ A279), G286 (= G301), R288 (= R303), V293 (≠ L308), D310 (= D325), I311 (= I326), D329 (= D344), V330 (≠ A345), M405 (= M416), G423 (≠ H434), G424 (= G435)
binding magnesium ion: D453 (= D465), N480 (= N492), H482 (≠ Y494)
binding 2-(2,2-difluoroethoxy)-N-(5,8-dimethoxy[1,2,4]triazolo[1,5-c]pyrimidin-2-yl)-6-(trifluoromethyl)benzenesulfonamide: M266 (= M281), D291 (= D306), R292 (= R307), M485 (= M497), W489 (= W501), S568 (= S573)
binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V400 (= V411), G401 (= G412), Q402 (= Q413), H403 (= H414), M428 (= M439), D453 (= D465), G454 (≠ A466), S455 (= S467), M458 (= M470), N480 (= N492), H482 (≠ Y494), L483 (≠ I495), G484 (= G496), M485 (= M497), V486 (= V498)

5k6tA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylamino-carbonyl-triazolinone herbicide, propoxycarbazone-sodium (see paper)
43% identity, 94% coverage: 24:575/587 of query aligns to 8:570/582 of 5k6tA

query
sites
5k6tA

P

P

T

D

E

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V

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K

G

G

S

A

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A

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A
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I

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-

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Q

E

E

L

L

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A

V

T

A

I

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V

E

E

N

N

L

L

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P

I

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T

K

V

V

A

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I

L

L

L

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N
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x
H

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x
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E

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D

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F

F

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E

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A

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N

R

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M

A

-

H

-

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-

F

-

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-

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A

A

A

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G

E

Y

D

D

E

W

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C

F

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P

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N

F

M

D

L

K

L

L

F

A

A

E

A

A

A

F

C

G

G

A

I

R

P

G

A

W

A

T

R

V

V

D

T

S

K

Y

K

E

A

E

D

V

L

P

R

D

E

V

A

I

I

E

Q

E

T

A

M

L

L

A

D

Y

T

D

P

G

G

P

P

S

Y

V

L

I

L

D

D

F

V

H

I

V

C

D

P

P
x
H

A

Q

E

E

N

H

V

V

Y

L

P

P

M

M

V

I

S

P

S
|
S

G

G

active site: Y33 (≠ V49), G35 (= G51), G36 (= G52), A37 (= A53), S38 (≠ I54), E59 (= E74), T82 (= T97), F121 (= F136), Q122 (= Q137), E123 (= E138), K171 (= K186), M266 (= M281), V293 (≠ L308), V400 (= V411), G426 (= G437), M428 (= M439), D453 (= D465), N480 (= N492), H482 (≠ Y494), L483 (≠ I495), M485 (= M497), V486 (= V498), W489 (= W501), H558 (≠ P563)
binding methyl 2-[(4-methyl-5-oxidanylidene-3-propoxy-1,2,4-triazol-1-yl)carbonylsulfamoyl]benzoate: H267 (= H282), R292 (= R307), M485 (= M497), W489 (= W501), S568 (= S573)
binding flavin-adenine dinucleotide: R161 (= R176), G222 (= G235), G223 (= G236), G224 (= G237), T246 (= T261), L247 (≠ M262), M248 (≠ P263), L264 (≠ A279), G286 (= G301), R288 (= R303), D290 (= D305), R292 (= R307), V293 (≠ L308), D310 (= D325), I311 (= I326), D329 (= D344), V330 (≠ A345), Q404 (= Q415), M405 (= M416), G423 (≠ H434)
binding magnesium ion: D453 (= D465), N480 (= N492), H482 (≠ Y494)
binding 2-{3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-methyl-2-oxo-2,3-dihydro-1,3-thiazol-5-yl}ethyl trihydrogendiphosphate: V400 (= V411), G401 (= G412), Q402 (= Q413), H403 (= H414), G426 (= G437), M428 (= M439), G452 (= G464), G454 (≠ A466), S455 (= S467), N480 (= N492), H482 (≠ Y494), L483 (≠ I495), G484 (= G496)

5k6rA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylamino-carbonyl-triazolinone herbicide, thiencarbazone-methyl (see paper)
43% identity, 94% coverage: 24:575/587 of query aligns to 8:570/582 of 5k6rA

query
sites
5k6rA

P

P

T

D

E

Q

V

P

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K

G

G

S

A

T

D

A

I

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V

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E

A

A

L

L

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E

N

R

A

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G

G

V

V

E

E

T

T

A

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F

G

A

V
x
Y

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G
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G

A
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A

I
x
S

M

M

P

E

I

I

Y

H

D

Q

A

A

L

L

Y

T

H

R

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S

I

I

R

R

H

N

V

V

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A

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H

H

E
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E

Q

Q

G

G

A

G

A

V

H

F

A

A

D

E

A

G

Y

Y

G

A

V

R

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S

R

S

G

G

D

K

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P

G

G

V

I

C

C

L

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A

A

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T

S

S

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G

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P

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A

A

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E

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G

active site: Y33 (≠ V49), G35 (= G51), G36 (= G52), A37 (= A53), S38 (≠ I54), E59 (= E74), T82 (= T97), F121 (= F136), Q122 (= Q137), E123 (= E138), K171 (= K186), M266 (= M281), V293 (≠ L308), V400 (= V411), G426 (= G437), M428 (= M439), D453 (= D465), N480 (= N492), H482 (≠ Y494), L483 (≠ I495), M485 (= M497), V486 (= V498), W489 (= W501), H558 (≠ P563)
binding methyl 4-[(3-methoxy-4-methyl-5-oxidanylidene-1,2,4-triazol-1-yl)carbonylsulfamoyl]-5-methyl-thiophene-3-carboxylate: R292 (= R307), W489 (= W501), S568 (= S573)
binding flavin-adenine dinucleotide: R161 (= R176), G222 (= G235), G223 (= G236), G224 (= G237), T246 (= T261), L247 (≠ M262), M248 (≠ P263), L264 (≠ A279), M266 (= M281), G286 (= G301), R288 (= R303), R292 (= R307), V293 (≠ L308), D310 (= D325), I311 (= I326), G328 (= G343), D329 (= D344), V330 (≠ A345), M405 (= M416), G423 (≠ H434)
binding magnesium ion: D453 (= D465), N480 (= N492), H482 (≠ Y494)
binding 2-{3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-methyl-2-oxo-2,3-dihydro-1,3-thiazol-5-yl}ethyl trihydrogendiphosphate: V400 (= V411), G401 (= G412), Q402 (= Q413), H403 (= H414), G426 (= G437), M428 (= M439), D453 (= D465), G454 (≠ A466), S455 (= S467), M458 (= M470), N480 (= N492), H482 (≠ Y494), L483 (≠ I495), G484 (= G496), M485 (= M497), V486 (= V498)

1z8nA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with an imidazolinone herbicide, imazaquin (see paper)
43% identity, 94% coverage: 24:575/587 of query aligns to 8:570/582 of 1z8nA

query
sites
1z8nA

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x
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G

active site: Y33 (≠ V49), G35 (= G51), G36 (= G52), A37 (= A53), S38 (≠ I54), E59 (= E74), T82 (= T97), F121 (= F136), Q122 (= Q137), E123 (= E138), K171 (= K186), M266 (= M281), V293 (≠ L308), V400 (= V411), G426 (= G437), M428 (= M439), D453 (= D465), N480 (= N492), H482 (≠ Y494), L483 (≠ I495), M485 (= M497), V486 (= V498), W489 (= W501), H558 (≠ P563)
binding 2-(4-isopropyl-4-methyl-5-oxo-4,5-dihydro-1h-imidazol-2-yl)quinoline-3-carboxylic acid: K135 (= K150), R161 (= R176), Y191 (≠ E204), R194 (= R207), D291 (= D306), R292 (= R307), D312 (= D327), W489 (= W501), G569 (= G574)
binding flavin-adenine dinucleotide: R161 (= R176), G222 (= G235), G224 (= G237), T246 (= T261), L247 (≠ M262), M248 (≠ P263), L264 (≠ A279), G265 (= G280), M266 (= M281), H267 (= H282), G286 (= G301), V287 (≠ T302), R288 (= R303), D290 (= D305), R292 (= R307), V293 (≠ L308), D310 (= D325), I311 (= I326), D329 (= D344), V330 (≠ A345), M405 (= M416), G423 (≠ H434), G424 (= G435)
binding magnesium ion: D453 (= D465), N480 (= N492)
binding thiamine diphosphate: V400 (= V411), G401 (= G412), Q402 (= Q413), H403 (= H414), G426 (= G437), M428 (= M439), G452 (= G464), G454 (≠ A466), S455 (= S467), N480 (= N492), H482 (≠ Y494), L483 (≠ I495), G484 (= G496), M485 (= M497), V486 (= V498)

1yi1A Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide, tribenuron methyl (see paper)
43% identity, 94% coverage: 24:575/587 of query aligns to 8:570/582 of 1yi1A

query
sites
1yi1A

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E

A

G

Y

Y

G

A

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R

S

G

G

D

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G

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C

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A

A

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G

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G

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A

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L

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I

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A

A

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D

A

A

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A

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V

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A

A

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A

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R

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L

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L

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K

D

D

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-

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L

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A

E

I

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N

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E

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A

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A

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L

L

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G

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-

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-

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M

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G

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V

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I

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I
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I

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V

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A

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-

L

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-

F

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N

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V

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G

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M

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A

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x
G

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M

G

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L

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P

A

A

I

I

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A

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V

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A

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N

D

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Q

E

E

L

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A

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A

I

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R

V

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E

N

N

L

L

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I

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V

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A

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I

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x
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x
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D

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F

F

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E

K

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A

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N

R

R

M

A

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H

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F

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-

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-

P

A

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W

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C

F

P

P

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N

F

M

D

L

K

L

L

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C

G

G

A

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A

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Y

K

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A

I

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M

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Y

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D

P

G

G

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P

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Y

V

L

I

L

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D

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I

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x
H

A

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H

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L

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V

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S
|
S

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G

active site: Y33 (≠ V49), G35 (= G51), G36 (= G52), A37 (= A53), S38 (≠ I54), E59 (= E74), T82 (= T97), F121 (= F136), Q122 (= Q137), E123 (= E138), K171 (= K186), M266 (= M281), V293 (≠ L308), V400 (= V411), G426 (= G437), M428 (= M439), D453 (= D465), N480 (= N492), H482 (≠ Y494), L483 (≠ I495), M485 (= M497), V486 (= V498), W489 (= W501), H558 (≠ P563)
binding methyl 2-[4-methoxy-6-methyl-1,3,5-trazin-2-yl(methyl)carbamoylsulfamoyl]benzoate: D291 (= D306), R292 (= R307), W489 (= W501), S568 (= S573)
binding flavin-adenine dinucleotide: R161 (= R176), G223 (= G236), G224 (= G237), T246 (= T261), L247 (≠ M262), M248 (≠ P263), M263 (≠ W278), L264 (≠ A279), G265 (= G280), M266 (= M281), H267 (= H282), G286 (= G301), V287 (≠ T302), R288 (= R303), D290 (= D305), V293 (≠ L308), D310 (= D325), I311 (= I326), D329 (= D344), V330 (≠ A345), M405 (= M416), G423 (≠ H434), G424 (= G435)
binding magnesium ion: D453 (= D465), N480 (= N492), H482 (≠ Y494)

1yi0A Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide, sulfometuron methyl (see paper)
43% identity, 94% coverage: 24:575/587 of query aligns to 8:570/582 of 1yi0A

query
sites
1yi0A

P

P

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A
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x
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I

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I

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E

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A

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E

A

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Y

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A

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C

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I

A

A

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T

S

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G

G

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P

G

G

A

A

T

T

N

N

L

L

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A

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D

A

A

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E

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R

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I
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I

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I

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F

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K

L

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G

A

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A

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A

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A

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M

L

L

A

D

Y

T

D

P

G

G

P

P

S

Y

V

L

I

L

D

D

F

V

H

I

V

C

D

P

P
x
H

A

Q

E

E

N

H

V

V

Y

L

P

P

M

M

V

I

S

P

S
|
S

G

G

active site: Y33 (≠ V49), G35 (= G51), G36 (= G52), A37 (= A53), S38 (≠ I54), E59 (= E74), T82 (= T97), F121 (= F136), Q122 (= Q137), E123 (= E138), K171 (= K186), M266 (= M281), V293 (≠ L308), V400 (= V411), G426 (= G437), M428 (= M439), D453 (= D465), N480 (= N492), H482 (≠ Y494), L483 (≠ I495), M485 (= M497), V486 (= V498), W489 (= W501), H558 (≠ P563)
binding methyl 2-[({[(4,6-dimethylpyrimidin-2-yl)amino]carbonyl}amino)sulfonyl]benzoate: D291 (= D306), R292 (= R307), W489 (= W501), S568 (= S573)
binding flavin-adenine dinucleotide: R161 (= R176), G222 (= G235), G223 (= G236), G224 (= G237), T246 (= T261), L247 (≠ M262), M248 (≠ P263), L264 (≠ A279), G265 (= G280), M266 (= M281), H267 (= H282), G286 (= G301), V287 (≠ T302), R288 (= R303), D290 (= D305), R292 (= R307), V293 (≠ L308), D310 (= D325), I311 (= I326), G328 (= G343), D329 (= D344), V330 (≠ A345), M405 (= M416), G423 (≠ H434), G424 (= G435)
binding magnesium ion: D453 (= D465), N480 (= N492), H482 (≠ Y494)

1yhzA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide, chlorsulfuron (see paper)
43% identity, 94% coverage: 24:575/587 of query aligns to 8:570/582 of 1yhzA

query
sites
1yhzA

P

P

T

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E

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G

S

A

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A

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A

A

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E

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R

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G

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E

E

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T

A

V

F

F

G

A

V
x
Y

Q

P

G
|
G

A
|
A

I
x
S

M

M

P

E

I

I

Y

H

D

Q

A

A

L

L

Y

T

H

R

S

S

D

S

-

S

I

I

R

R

H

N

V

V

T

L

M

P

A

R

H

H

E
|
E

Q

Q

G

G

A

G

A

V

H

F

A

A

D

E

A

G

Y

Y

G

A

V

R

V

S

R

S

G

G

D

K

P

P

G

G

V

I

C

C

L

I

A

A

T
|
T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

L

L

V

V

T

S

G

G

I

L

A

A

D

D

A

A

N

L

M

L

D

D

S

S

D

V

A

P

V

L

L

V

A

A

L

I

T

T

G

G

Q

Q

V

V

P

P

S

R

D

R

M

M

V

I

G

G

S

T

D

D

A

A

F
|
F

Q
|
Q

E
|
E

T

T

D

P

T

I

T

V

G

E

V

V

T

T

A

R

P

S

I

I

T

T

K

K

H

H

N

N

Y

Y

F

L

A

V

S

M

S

D

P

V

D

E

T

D

V

I

G

P

D

R

T

I

V

I

G

E

E

E

A

A

F

F

A

F

L

L

A

A

A

T

E

S

G

G

R
|
R

P

P

G

G

P

P

T

V

L

L

V

V

D

D

L

V

P

P

K
|
K

D

D

V

I

-

Q

S

Q

L

L

N

A

E

I

T

P

D

N

T

W

E

E

S

Q

G

A

P

M

A

R

Q

L

P

P

P

G

E

Y

T

M

C

S

R

R

A

M

R

P

T

K

D

P

P

P

A

E

D

D

S

S

Q

H

V

L

E

E

A

Q

A

I

A

V

R

R

A

L

I

I

E

S

R

E

A

S

E

K

K

K

P

P

L

V

L

L

L

Y

F

V

G

G

G
|
G

V

C

V

L

K

N

A

S

D

-

A

S

T

D

E

E

V

L

A

G

R

R

Q

-

F

F

A

V

T

E

E

L

H

T

R

G

I

I

P

P

V

V

V

A

T

S

T
|
T

M
x
L

P
x
M

G

G

I

L

G

G

A

S

M

Y

P

P

E

C

D

D

H

D

E

E

L

L

C

S

L

L

S

H

W

M

A
x
L

G

G

M
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M

H
|
H

G

G

T

T

G

V

Y

Y

A

A

N

N

M

Y

A

A

I

V

T

E

H

H

T

S

D

D

C

L

L

L

I

L

A

A

V

F

G
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G

T
x
V

R
|
R

F

F

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|
D

R
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R

L
x
V

T

T

G

G

G

K

I

L

D

E

T

A

F

F

A

A

P

S

E

R

A

A

E

K

V

I

V

V

H

H

I

I

D
|
D

I
|
I

D

D

P

S

A

A

E

E

I

I

S

G

K

K

N

N

V

K

H

T

A

P

D

H

Y

V

P

S

V

V

V

C

G

G

D
|
D

A
x
V

G

K

R

L

A

A

I

L

E

Q

R

G

V

M

D

N

A

K

E

V

L

L

D

E

E

N

G

R

P

A

D

E

A

E

R

L

E

K

-

L

-

D

-

F

-

G

-

V

W

W

V

R

D

N

Q

E

C

L

L

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A

V

W

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K

K

D

Q

D

K

Y

F

P

P

M

L

D

S

Y

F

A

K

A

T

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-

D

F

N

G

E

E

P

A

L

I

K

P

P

P

Q

Q

F

Y

V

A

V

I

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K

A

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L

L

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D

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A

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T

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D

D

G

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K

T

A

I

I

V

I

T

S

S

T

G

G

V
|
V

G

G

Q

Q

H

H

Q
|
Q

M
|
M

W

W

A

A

Q

Q

Y

F

W

Y

T

N

F

Y

T

K

E

K

P

P

R

R

R

Q

W

W

V

L

S

S

H
x
G

G
|
G

L

L

G
|
G

T

A

M
|
M

G

G

Y

F

G

G

L

L

P

P

A

A

I

I

G

G

A

A

R

S

V

V

A

-

A

A

D

N

D

P

D

D

E

A

T

I

V

V

C

D

V

I

D

D

G

G

D
|
D

A

G

S

S

F

F

L

I

M

M

T

N

I

V

Q

Q

E

E

L

L

S

A

V

T

A

I

V

R

R

V

E

E

N

N

L

L

D

P

I

V

T

K

V

V

A

L

I

L

L

L

N

N

N
|
N

E

Q

Y
x
H

I
x
L

G

G

M
|
M

V
|
V

R

M

Q

Q

W
|
W

Q

E

D

D

A

R

F

F

F

Y

E

K

G

A

R

N

R

R

M

A

-

H

-

T

-

F

-

L

-

G

-

D

-

P

A

A

A

Q

G

E

Y

D

D

E

W

I

C

F

P

P

Q

N

F

M

D

L

K

L

L

F

A

A

E

A

A

A

F

C

G

G

A

I

R

P

G

A

W

A

T

R

V

V

D

T

S

K

Y

K

E

A

E

D

V

L

P

R

D

E

V

A

I

I

E

Q

E

T

A

M

L

L

A

D

Y

T

D

P

G

G

P

P

S

Y

V

L

I

L

D

D

F

V

H

I

V

C

D

P

P
x
H

A

Q

E

E

N

H

V

V

Y

L

P

P

M

M

V

I

S

P

S
|
S

G

G

active site: Y33 (≠ V49), G35 (= G51), G36 (= G52), A37 (= A53), S38 (≠ I54), E59 (= E74), T82 (= T97), F121 (= F136), Q122 (= Q137), E123 (= E138), K171 (= K186), M266 (= M281), V293 (≠ L308), V400 (= V411), G426 (= G437), M428 (= M439), D453 (= D465), N480 (= N492), H482 (≠ Y494), L483 (≠ I495), M485 (= M497), V486 (= V498), W489 (= W501), H558 (≠ P563)
binding 1-(2-chlorophenylsulfonyl)-3-(4-methoxy-6-methyl-l,3,5-triazin-2-yl)urea: D291 (= D306), R292 (= R307), M485 (= M497), W489 (= W501), S568 (= S573)
binding flavin-adenine dinucleotide: R161 (= R176), G223 (= G236), G224 (= G237), T246 (= T261), L247 (≠ M262), M248 (≠ P263), L264 (≠ A279), M266 (= M281), H267 (= H282), G286 (= G301), V287 (≠ T302), R288 (= R303), D290 (= D305), V293 (≠ L308), D310 (= D325), I311 (= I326), D329 (= D344), V330 (≠ A345), Q404 (= Q415), M405 (= M416), G423 (≠ H434), G424 (= G435)
binding magnesium ion: D453 (= D465), N480 (= N492), H482 (≠ Y494)

1yhyA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide, metsulfuron methyl (see paper)
43% identity, 94% coverage: 24:575/587 of query aligns to 8:570/582 of 1yhyA

query
sites
1yhyA

P

P

T

D

E

Q

V

P

T

R

S

K

G

G

S

A

T

D

A

I

V

L

V

V

R

E

A

A

L

L

E

E

N

R

A

Q

G

G

V

V

E

E

T

T

A

V

F

F

G

A

V
x
Y

Q

P

G
|
G

A
|
A

I
x
S

M

M

P

E

I

I

Y

H

D

Q

A

A

L

L

Y

T

H

R

S

S

D

S

-

S

I

I

R

R

H

N

V

V

T

L

M

P

A

R

H

H

E
|
E

Q

Q

G

G

A

G

A

V

H

F

A

A

D

E

A

G

Y

Y

G

A

V

R

V

S

R

S

G

G

D

K

P

P

G

G

V

I

C

C

L

I

A

A

T
|
T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

L

L

V

V

T

S

G

G

I

L

A

A

D

D

A

A

N

L

M

L

D

D

S

S

D

V

A

P

V

L

L

V

A

A

L

I

T

T

G

G

Q

Q

V

V

P

P

S

R

D

R

M

M

V

I

G

G

S

T

D

D

A

A

F
|
F

Q
|
Q

E
|
E

T

T

D

P

T

I

T

V

G

E

V

V

T

T

A

R

P

S

I

I

T

T

K

K

H

H

N

N

Y

Y

F

L

A

V

S

M

S

D

P

V

D

E

T

D

V

I

G

P

D

R

T

I

V

I

G

E

E

E

A

A

F

F

A

F

L

L

A

A

A

T

E

S

G

G

R
|
R

P

P

G

G

P

P

T

V

L

L

V

V

D

D

L

V

P

P

K
|
K

D

D

V

I

-

Q

S

Q

L

L

N

A

E

I

T

P

D

N

T

W

E

E

S

Q

G

A

P

M

A

R

Q

L

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P

P

G

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Y

T

M

C

S

R

R

A

M

R

P

T

K

D

P

P

P

A

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D

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S

Q

H

V

L

E

E

A

Q

A

I

A

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R

A

L

I

I

E

S

R

E

A

S

E

K

K

K

P

P

L

V

L

L

L

Y

F

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G

V

C

V

L

K

N

A

S

D

-

A

S

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D

E

E

V

L

A

G

R

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Q

-

F

F

A

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E

E

L

H

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G

I

I

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P

V

V

V

A

T

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|
T

M
x
L

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x
M

G

G

I

L

G

G

A

S

M

Y

P

P

E

C

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D

H

D

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E

L

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L

L

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H

W

M

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x
L

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G

M
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M

H
|
H

G

G

T

T

G

V

Y

Y

A

A

N

N

M

Y

A

A

I

V

T

E

H

H

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S

D

D

C

L

L

L

I

L

A

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G

T
x
V

R
|
R

F

F

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|
D

R
|
R

L
x
V

T

T

G

G

G

K

I

L

D

E

T

A

F

F

A

A

P

S

E

R

A

A

E

K

V

I

V

V

H

H

I

I

D
|
D

I
|
I

D

D

P

S

A

A

E

E

I

I

S

G

K

K

N

N

V

K

H

T

A

P

D

H

Y

V

P

S

V

V

V

C

G

G

D
|
D

A
x
V

G

K

R

L

A

A

I

L

E

Q

R

G

V

M

D

N

A

K

E

V

L

L

D

E

E

N

G

R

P

A

D

E

A

E

R

L

E

K

-

L

-

D

-

F

-

G

-

V

W

W

V

R

D

N

Q

E

C

L

L

N

A

V

W

Q

K

K

D

Q

D

K

Y

F

P

P

M

L

D

S

Y

F

A

K

A

T

P

-

D

F

N

G

E

E

P

A

L

I

K

P

P

P

Q

Q

F

Y

V

A

V

I

E

K

A

V

L

L

D

D

E

E

A

L

T

T

P

D

D

G

E

K

T

A

I

I

V

I

T

S

S

T

G

G

V
|
V

G

G

Q

Q

H

H

Q
|
Q

M
|
M

W

W

A

A

Q

Q

Y

F

W

Y

T

N

F

Y

T

K

E

K

P

P

R

R

R

Q

W

W

V

L

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S

H
x
G

G
|
G

L

L

G
|
G

T

A

M
|
M

G

G

Y

F

G

G

L

L

P

P

A

A

I

I

G

G

A

A

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S

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V

A

-

A

A

D

N

D

P

D

D

E

A

T

I

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C

D

V

I

D

D

G

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D
|
D

A

G

S

S

F

F

L

I

M

M

T

N

I

V

Q

Q

E

E

L

L

S

A

V

T

A

I

V

R

R

V

E

E

N

N

L

L

D

P

I

V

T

K

V

V

A

L

I

L

L

L

N

N

N
|
N

E

Q

Y
x
H

I
x
L

G

G

M
|
M

V
|
V

R

M

Q

Q

W
|
W

Q

E

D

D

A

R

F

F

F

Y

E

K

G

A

R

N

R

R

M

A

-

H

-

T

-

F

-

L

-

G

-

D

-

P

A

A

A

Q

G

E

Y

D

D

E

W

I

C

F

P

P

Q

N

F

M

D

L

K

L

L

F

A

A

E

A

A

A

F

C

G

G

A

I

R

P

G

A

W

A

T

R

V

V

D

T

S

K

Y

K

E

A

E

D

V

L

P

R

D

E

V

A

I

I

E

Q

E

T

A

M

L

L

A

D

Y

T

D

P

G

G

P

P

S

Y

V

L

I

L

D

D

F

V

H

I

V

C

D

P

P
x
H

A

Q

E

E

N

H

V

V

Y

L

P

P

M

M

V

I

S

P

S
|
S

G

G

active site: Y33 (≠ V49), G35 (= G51), G36 (= G52), A37 (= A53), S38 (≠ I54), E59 (= E74), T82 (= T97), F121 (= F136), Q122 (= Q137), E123 (= E138), K171 (= K186), M266 (= M281), V293 (≠ L308), V400 (= V411), G426 (= G437), M428 (= M439), D453 (= D465), N480 (= N492), H482 (≠ Y494), L483 (≠ I495), M485 (= M497), V486 (= V498), W489 (= W501), H558 (≠ P563)
binding methyl 2-[({[(4-methoxy-6-methyl-1,3,5-triazin-2-yl)amino]carbonyl}amino)sulfonyl]benzoate: D291 (= D306), R292 (= R307), V486 (= V498), W489 (= W501), S568 (= S573)
binding flavin-adenine dinucleotide: R161 (= R176), G222 (= G235), G223 (= G236), G224 (= G237), T246 (= T261), L247 (≠ M262), M248 (≠ P263), L264 (≠ A279), G265 (= G280), M266 (= M281), H267 (= H282), G286 (= G301), V287 (≠ T302), R288 (= R303), D290 (= D305), V293 (≠ L308), D310 (= D325), I311 (= I326), D329 (= D344), V330 (≠ A345), Q404 (= Q415), M405 (= M416), G423 (≠ H434), G424 (= G435)
binding magnesium ion: D453 (= D465), N480 (= N492), H482 (≠ Y494)

1ybhA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide chlorimuron ethyl (see paper)
43% identity, 94% coverage: 24:575/587 of query aligns to 8:570/582 of 1ybhA

query
sites
1ybhA

P

P

T

D

E

Q

V

P

T

R

S

K

G

G

S

A

T

D

A

I

V

L

V

V

R

E

A

A

L

L

E

E

N

R

A

Q

G

G

V

V

E

E

T

T

A

V

F

F

G

A

V
x
Y

Q

P

G
|
G

A
|
A

I
x
S

M

M

P

E

I

I

Y

H

D

Q

A

A

L

L

Y

T

H

R

S

S

D

S

-

S

I

I

R

R

H

N

V

V

T

L

M

P

A

R

H

H

E
|
E

Q

Q

G

G

A

G

A

V

H

F

A

A

D

E

A

G

Y

Y

G

A

V

R

V

S

R

S

G

G

D

K

P

P

G

G

V

I

C

C

L

I

A

A

T
|
T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

L

L

V

V

T

S

G

G

I

L

A

A

D

D

A

A

N

L

M

L

D

D

S

S

D

V

A

P

V

L

L

V

A

A

L

I

T

T

G

G

Q

Q

V

V

P

P

S

R

D

R

M

M

V

I

G

G

S

T

D

D

A

A

F
|
F

Q
|
Q

E
|
E

T

T

D

P

T

I

T

V

G

E

V

V

T

T

A

R

P

S

I

I

T

T

K

K

H

H

N

N

Y

Y

F

L

A

V

S

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R

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K

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-

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x
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I

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-

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x
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E

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N

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A

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x
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M

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D

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A

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N

R

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A

-

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-

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-

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-

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-

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-

P

A

A

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A

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G

active site: Y33 (≠ V49), G35 (= G51), G36 (= G52), A37 (= A53), S38 (≠ I54), E59 (= E74), T82 (= T97), F121 (= F136), Q122 (= Q137), E123 (= E138), K171 (= K186), M266 (= M281), V293 (≠ L308), V400 (= V411), G426 (= G437), M428 (= M439), D453 (= D465), N480 (= N492), H482 (≠ Y494), L483 (≠ I495), M485 (= M497), V486 (= V498), W489 (= W501), H558 (≠ P563)
binding 2-[[[[(4-chloro-6-methoxy-2-pyrimidinyl)amino]carbonyl]amino]sulfonyl]benzoic acid ethyl ester: M266 (= M281), D291 (= D306), R292 (= R307), M485 (= M497), W489 (= W501), S568 (= S573)
binding flavin-adenine dinucleotide: R161 (= R176), G223 (= G236), G224 (= G237), T246 (= T261), L247 (≠ M262), M248 (≠ P263), L264 (≠ A279), M266 (= M281), H267 (= H282), G286 (= G301), V287 (≠ T302), R288 (= R303), D290 (= D305), V293 (≠ L308), D310 (= D325), I311 (= I326), D329 (= D344), V330 (≠ A345), Q404 (= Q415), M405 (= M416), G423 (≠ H434), G424 (= G435)
binding magnesium ion: D453 (= D465), N480 (= N492), H482 (≠ Y494)

P17597 Acetolactate synthase, chloroplastic; AtALS; Acetohydroxy-acid synthase; Protein CHLORSULFURON RESISTANT 1; EC 2.2.1.6 from Arabidopsis thaliana (Mouse-ear cress) (see 8 papers)
43% identity, 94% coverage: 24:575/587 of query aligns to 93:655/670 of P17597

query
sites
P17597

P

P

T

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E

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G

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A

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E

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F

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E

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N

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A

-

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-

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-

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A

A

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L

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S
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S

G

G

A122 (= A53) mutation to V: Reduced catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.
M124 (= M55) mutation to E: Reduced catalytic activity. Resistant to imidazolinone herbicides and reduced sensitivity to sulfonylurea herbicides.; mutation to I: No effect on catalytic activity. Increased resistance to imidazolinone herbicides.
E144 (= E74) binding thiamine diphosphate
S186 (= S116) binding FAD
P197 (= P127) mutation to S: In csr1-1/GH50; resistant to sulfonylurea but not to imidazolinone herbicides.
R199 (≠ D129) mutation R->A,E: No effect on catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.
Q207 (= Q137) binding thiamine diphosphate
K220 (= K150) binding (R)-imazaquin
R246 (= R176) binding (R)-imazaquin; binding FAD
K256 (= K186) binding chlorimuron-ethyl
G308 (= G236) binding FAD
TL 331:332 (≠ TM 261:262) binding FAD
C340 (≠ E270) modified: Cysteine sulfinic acid (-SO2H)
LGMH 349:352 (≠ AGMH 279:282) binding FAD
GVRFD 371:375 (≠ GTRFD 301:305) binding FAD
DR 376:377 (= DR 306:307) binding chlorimuron-ethyl
DI 395:396 (= DI 325:326) binding FAD
DV 414:415 (≠ DA 344:345) binding FAD
QH 487:488 (= QH 413:414) binding thiamine diphosphate
GG 508:509 (≠ HG 434:435) binding FAD
GAM 511:513 (≠ GTM 437:439) binding thiamine diphosphate
D538 (= D465) binding Mg(2+)
DGS 538:540 (≠ DAS 465:467) binding thiamine diphosphate
N565 (= N492) binding Mg(2+)
NQHLGM 565:570 (≠ NEYIGM 492:497) binding thiamine diphosphate
H567 (≠ Y494) binding Mg(2+)
W574 (= W501) binding chlorimuron-ethyl; mutation to L: Increased catalytic activity. Resistant to imidazolinone and sulfonylurea herbicides.; mutation to S: Slightly decreased catalytic activity. Resistant to imidazolinone and sulfonylurea herbicides.
S653 (= S573) binding chlorimuron-ethyl; mutation to A: No effect on catalytic activity or sensitivity to herbicides.; mutation to F: No effect on catalytic activity. Resistant to imidazolinone herbicides and also slightly sulfonylurea-resistant.; mutation to N: In csr1-2/GH90; no effect on catalytic activity. Resistant to imidazolinone but not to sulfonylurea herbicides.; mutation to T: No effect on catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.

5k3sA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a pyrimidinyl-benzoate herbicide, bispyribac-sodium (see paper)
43% identity, 94% coverage: 24:575/587 of query aligns to 8:570/583 of 5k3sA

query
sites
5k3sA

P

P

T

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E

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G

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F

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x
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A
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M

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A

A

L

L

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E
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E

Q

Q

G

G

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G

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F

A

A

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E

A

G

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Y

G

A

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R

S

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G

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P

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C

C

L

I

A

A

T
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T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

L

L

V

V

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G

G

I

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A

A

D

D

A

A

N

L

M

L

D

D

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S

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A

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L

L

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A

L

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G

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G

G

S

T

D

D

A

A

F
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F

Q
|
Q

E
|
E

T

T

D

P

T

I

T

V

G

E

V

V

T

T

A

R

P

S

I

I

T

T

K

K

H

H

N

N

Y

Y

F

L

A

V

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M

S

D

P

V

D

E

T

D

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I

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P

D

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T

I

V

I

G

E

E

E

A

A

F

F

A

F

L

L

A

A

A

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G

G

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R

P

P

G

G

P

P

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L

L

V

V

D

D

L

V

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P

K
|
K

D

D

V

I

-

Q

S

Q

L

L

N

A

E

I

T

P

D

N

T

W

E

E

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Q

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A

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A

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P

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E

Y

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A

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A

I

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A

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I

I

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E

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P

L

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L

L

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Y

F

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G

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-

A

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T

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E

E

V

L

A

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-

F

F

A

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E

E

L

H

T

R

G

I

I

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P

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V

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T

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x
L

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x
M

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G

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Y

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C

D

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H

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E

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x
L

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M

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Y

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Y

A

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R

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x
V

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A

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D

I
|
I

D

D

P

S

A

A

E

E

I

I

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K

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N

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K

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T

A

P

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Y

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x
V

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A

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L

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N

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A

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A

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R

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E

K

-

L

-

D

-

F

-

G

-

V

W

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E

C

L

L

N

A

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K

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Q

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M

L

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S

Y

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-

D

F

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E

P

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Q

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Y

V

A

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A

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M

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W

A

A

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Y

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K

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Q

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x
G

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M

G

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G

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L

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P

A

A

I

I

G

G

A

A

R

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V

A

-

A

A

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N

D

P

D

D

E

A

T

I

V

V

C

D

V

I

D

D

G

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D

A
x
G

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S

F

F

L

I

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M

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N

I

V

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Q

E

E

L

L

S

A

V

T

A

I

V

R

R

V

E

E

N

N

L

L

D

P

I

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T

K

V

V

A

L

I

L

L

L

N

N

N
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E

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x
H

I
x
L

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G

M
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M

V
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V

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M

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W

Q

E

D

D

A

R

F

F

F

Y

E

K

G

A

R

N

R

R

M

A

-

H

-

T

-

F

-

L

-

G

-

D

-

P

A

A

A

Q

G

E

Y

D

D

E

W

I

C

F

P

P

Q

N

F

M

D

L

K

L

L

F

A

A

E

A

A

A

F

C

G

G

A

I

R

P

G

A

W

A

T

R

V

V

D

T

S

K

Y

K

E

A

E

D

V

L

P

R

D

E

V

A

I

I

E

Q

E

T

A

M

L

L

A

D

Y

T

D

P

G

G

P

P

S

Y

V

L

I

L

D

D

F

V

H

I

V

C

D

P

P
x
H

A

Q

E

E

N

H

V

V

Y

L

P

P

M

M

V

I

S

P

S

S

G
|
G

G

G

active site: Y33 (≠ V49), G35 (= G51), G36 (= G52), A37 (= A53), S38 (≠ I54), E59 (= E74), T82 (= T97), F121 (= F136), Q122 (= Q137), E123 (= E138), K171 (= K186), M266 (= M281), V293 (≠ L308), V400 (= V411), G426 (= G437), M428 (= M439), D453 (= D465), N480 (= N492), H482 (≠ Y494), L483 (≠ I495), M485 (= M497), V486 (= V498), W489 (= W501), H558 (≠ P563)
binding 2,6-bis[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid: R292 (= R307), M485 (= M497), W489 (= W501), G569 (= G574)
binding flavin-adenine dinucleotide: R161 (= R176), G222 (= G235), G223 (= G236), G224 (= G237), T246 (= T261), L247 (≠ M262), M248 (≠ P263), L264 (≠ A279), M266 (= M281), G286 (= G301), R288 (= R303), D290 (= D305), V293 (≠ L308), D310 (= D325), I311 (= I326), D329 (= D344), V330 (≠ A345), M405 (= M416), G423 (≠ H434)
binding magnesium ion: D453 (= D465), N480 (= N492), H482 (≠ Y494)
binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V400 (= V411), G401 (= G412), Q402 (= Q413), H403 (= H414), G426 (= G437), M428 (= M439), D453 (= D465), G454 (≠ A466), S455 (= S467), N480 (= N492), H482 (≠ Y494), L483 (≠ I495), G484 (= G496), M485 (= M497), V486 (= V498)

5k2oA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a pyrimidinyl-benzoate herbicide, pyrithiobac (see paper)
43% identity, 94% coverage: 24:575/587 of query aligns to 8:570/585 of 5k2oA

query
sites
5k2oA

P

P

T

D

E

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V

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T

R

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K

G

G

S

A

T

D

A

I

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V

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A

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L

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E

N

R

A

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G

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V

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E

T

T

A

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F

G

A

V
x
Y

Q

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G
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G

A
|
A

I
x
S

M

M

P

E

I

I

Y

H

D

Q

A

A

L

L

Y

T

H

R

S

S

D

S

-

S

I

I

R

R

H

N

V

V

T

L

M

P

A

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H

H

E
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E

Q

Q

G

G

A

G

A

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H

F

A

A

D

E

A

G

Y

Y

G

A

V

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V

S

R

S

G

G

D

K

P

P

G

G

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I

C

C

L

I

A

A

T
|
T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

L

L

V

V

T

S

G

G

I

L

A

A

D

D

A

A

N

L

M

L

D

D

S

S

D

V

A

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V

L

L

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A

A

L

I

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G

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Q

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V

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R

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M

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I

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G

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A

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F

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E
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E

T

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I

T

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G

E

V

V

T

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A

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I

I

T

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K

K

H

H

N

N

Y

Y

F

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S

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D

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I

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E

E

E

A

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A

F

L

L

A

A

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R
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R

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G

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L

L

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D

L

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K
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K

D

D

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I

-

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L

N

A

E

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N

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A

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K

P

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L

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G

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N

A

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A

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-

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F

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T

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x
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x
M

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H

D

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L

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M

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x
L

G

G

M
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M

H

H

G

G

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T

G

V

Y

Y

A

A

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M

Y

A

A

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C

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G

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R

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D

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R

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x
V

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G

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I

I

D
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D

I
|
I

D

D

P

S

A

A

E

E

I

I

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G

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K

N

N

V

K

H

T

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D

A
x
V

G

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A

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I

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R

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A

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A

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R

L

E

K

-

L

-

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-

F

-

G

-

V

W

W

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Q

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C

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N

A

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K

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Q

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A

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-

D

F

N

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P

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Q

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Y

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A

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E

A

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V

G
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G

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H
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H

Q
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M
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M

W

W

A

A

Q

Q

Y

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Y

T

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F

Y

T

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E

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P

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W

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x
G

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G
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G

T

A

M
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M

G

G

Y

F

G

G

L

L

P

P

A

A

I

I

G

G

A

A

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S

V

V

A

-

A

A

D

N

D

P

D

D

E

A

T

I

V

V

C

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V

I

D

D

G

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D
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D

A
x
G

S
|
S

F

F

L

I

M

M

T

N

I

V

Q

Q

E

E

L

L

S

A

V

T

A

I

V

R

R

V

E

E

N

N

L

L

D

P

I

V

T

K

V

V

A

L

I

L

L

L

N

N

N
|
N

E

Q

Y
x
H

I
x
L

G
|
G

M
|
M

V
|
V

R

M

Q

Q

W
|
W

Q

E

D

D

A

R

F

F

F

Y

E

K

G

A

R

N

R

R

M

A

-

H

-

T

-

F

-

L

-

G

-

D

-

P

A

A

A

Q

G

E

Y

D

D

E

W

I

C

F

P

P

Q

N

F

M

D

L

K

L

L

F

A

A

E

A

A

A

F

C

G

G

A

I

R

P

G

A

W

A

T

R

V

V

D

T

S

K

Y

K

E

A

E

D

V

L

P

R

D

E

V

A

I

I

E

Q

E

T

A

M

L

L

A

D

Y

T

D

P

G

G

P

P

S

Y

V

L

I

L

D

D

F

V

H

I

V

C

D

P

P
x
H

A

Q

E

E

N

H

V

V

Y

L

P

P

M

M

V

I

S

P

S
|
S

G

G

active site: Y33 (≠ V49), G35 (= G51), G36 (= G52), A37 (= A53), S38 (≠ I54), E59 (= E74), T82 (= T97), F121 (= F136), Q122 (= Q137), E123 (= E138), K171 (= K186), M266 (= M281), V293 (≠ L308), V400 (= V411), G426 (= G437), M428 (= M439), D453 (= D465), N480 (= N492), H482 (≠ Y494), L483 (≠ I495), M485 (= M497), V486 (= V498), W489 (= W501), H558 (≠ P563)
binding 2-chloranyl-6-(4,6-dimethoxypyrimidin-2-yl)sulfanyl-benzoic acid: M266 (= M281), R292 (= R307), W489 (= W501), S568 (= S573)
binding flavin-adenine dinucleotide: R161 (= R176), G222 (= G235), G223 (= G236), G224 (= G237), T246 (= T261), L247 (≠ M262), M248 (≠ P263), L264 (≠ A279), G286 (= G301), R288 (= R303), D290 (= D305), V293 (≠ L308), D310 (= D325), I311 (= I326), D329 (= D344), V330 (≠ A345), Q404 (= Q415), M405 (= M416), G423 (≠ H434)
binding magnesium ion: D453 (= D465), N480 (= N492), H482 (≠ Y494)
binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V400 (= V411), G401 (= G412), Q402 (= Q413), H403 (= H414), M428 (= M439), D453 (= D465), G454 (≠ A466), S455 (= S467), N480 (= N492), H482 (≠ Y494), L483 (≠ I495), G484 (= G496), M485 (= M497), V486 (= V498)

P09342 Acetolactate synthase 1, chloroplastic; ALS I; Acetohydroxy-acid synthase I; Acetolactate synthase I; EC 2.2.1.6 from Nicotiana tabacum (Common tobacco) (see 2 papers)
44% identity, 94% coverage: 24:576/587 of query aligns to 90:653/667 of P09342

query
sites
P09342

P

P

T

D

E

E

V

P

T

R

S

K

G

G

S

S

T

D

A

V

V

L

V

V

R

E

A

A

L

L

E

E

N

R

A

E

G

G

V

V

E

T

T

D

A

V

F

F

G

A

V

Y

Q

P

G

G

A

A

I

S

M

M

P

E

I

I

Y

H

D

Q

A

A

L

L

Y

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H

R

S

S

D

S

I

I

-

I

R

R

H

N

V

V

T

L

M

P

A

R

H

H

E

E

Q

Q

G

G

A

G

A

V

H

F

A

A

D

E

A

G

Y

Y

G

A

V

R

V

A

R

T

G

G

D

F

P

P

G

G

V

V

C
|
C

L

I

A

A

T

T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

L

L

V

V

T

S

G

G

I

L

A

A

D

D

A

A

N

L

M

L

D

D

S

S

D

V

A

P

V

I

L

V

A

A

L

I

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G

Q

Q

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V

P
|
P

S

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D

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C161 (= C94) modified: Disulfide link with 307
P194 (= P127) mutation to Q: In C3; highly resistant to sulfonylurea herbicides.
C307 (≠ V238) modified: Disulfide link with 161

7tzzA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase p197t mutant in complex with bispyribac-sodium (see paper)
43% identity, 94% coverage: 24:575/587 of query aligns to 8:570/582 of 7tzzA

query
sites
7tzzA

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binding 2,6-bis[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid: M266 (= M281), R292 (= R307), W489 (= W501), S568 (= S573)
binding 2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-[(1~{S})-1-(dioxidanyl)-1-oxidanyl-ethyl]-4-methyl-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: V400 (= V411), G401 (= G412), Q402 (= Q413), H403 (= H414), G426 (= G437), M428 (= M439), G452 (= G464), D453 (= D465), G454 (≠ A466), S455 (= S467), L483 (≠ I495), G484 (= G496), M485 (= M497), V486 (= V498)
binding flavin-adenine dinucleotide: R161 (= R176), G222 (= G235), G223 (= G236), G224 (= G237), T246 (= T261), L247 (≠ M262), M248 (≠ P263), M263 (≠ W278), L264 (≠ A279), M266 (= M281), H267 (= H282), G286 (= G301), R288 (= R303), V293 (≠ L308), D310 (= D325), I311 (= I326), D329 (= D344), V330 (≠ A345), M405 (= M416), G423 (≠ H434)
binding magnesium ion: A37 (= A53), T82 (= T97), S83 (= S98), Q122 (= Q137), Y381 (≠ F392), D453 (= D465), M458 (= M470), Q461 (= Q473), N480 (= N492), H482 (≠ Y494), K533 (≠ Y538)

1n0hA Crystal structure of yeast acetohydroxyacid synthase in complex with a sulfonylurea herbicide, chlorimuron ethyl (see paper)
42% identity, 93% coverage: 38:580/587 of query aligns to 20:576/599 of 1n0hA

query
sites
1n0hA

L

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active site: Y31 (≠ V49), G33 (= G51), G34 (= G52), A35 (= A53), I36 (= I54), E57 (= E74), T80 (= T97), F119 (= F136), Q120 (= Q137), E121 (= E138), K169 (= K186), R230 (≠ E245), M266 (= M281), V293 (≠ L308), V409 (= V411), L434 (= L436), G435 (= G437), M437 (= M439), D462 (= D465), N489 (= N492), E491 (≠ Y494), Q492 (≠ I495), M494 (= M497), V495 (= V498), W498 (= W501), L520 (= L524), G525 (= G529), L526 (≠ A530), K559 (≠ P563)
binding 4-{[(4'-amino-2'-methylpyrimidin-5'-yl)methyl]amino}pent-3-enyl diphosphate: V409 (= V411), G410 (= G412), Q411 (= Q413), H412 (= H414), G435 (= G437), M437 (= M439), G461 (= G464), D462 (= D465), A463 (= A466), S464 (= S467), M467 (= M470), N489 (= N492), E491 (≠ Y494), Q492 (≠ I495), G493 (= G496), V495 (= V498)
binding 2-[[[[(4-chloro-6-methoxy-2-pyrimidinyl)amino]carbonyl]amino]sulfonyl]benzoic acid ethyl ester: G34 (= G52), A35 (= A53), V109 (= V126), P110 (= P127), F119 (= F136), K169 (= K186), M266 (= M281), D291 (= D306), R292 (= R307), V495 (= V498), W498 (= W501)
binding flavin-adenine dinucleotide: R159 (= R176), G219 (= G235), A220 (≠ G236), G221 (= G237), N224 (≠ K240), T246 (= T261), L247 (≠ M262), Q248 (≠ P263), L264 (≠ A279), G265 (= G280), M266 (= M281), H267 (= H282), G286 (= G301), A287 (≠ T302), R288 (= R303), D290 (= D305), R292 (= R307), V293 (≠ L308), E319 (≠ D325), V320 (≠ I326), N324 (≠ E330), G337 (= G343), D338 (= D344), A339 (= A345), M414 (= M416), G432 (≠ H434), G433 (= G435)
binding magnesium ion: D462 (= D465), N489 (= N492), E491 (≠ Y494)
binding thiamine diphosphate: Y31 (≠ V49), E57 (= E74), P83 (= P100)

1t9dA Crystal structure of yeast acetohydroxyacid synthase in complex with a sulfonylurea herbicide, metsulfuron methyl (see paper)
41% identity, 94% coverage: 29:580/587 of query aligns to 9:573/596 of 1t9dA

query
sites
1t9dA

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active site: Y29 (≠ V49), G31 (= G51), G32 (= G52), A33 (= A53), I34 (= I54), E55 (= E74), T78 (= T97), F117 (= F136), Q118 (= Q137), E119 (= E138), K167 (= K186), R227 (≠ E245), M263 (= M281), V290 (≠ L308), V406 (= V411), L431 (= L436), G432 (= G437), M434 (= M439), D459 (= D465), N486 (= N492), E488 (≠ Y494), Q489 (≠ I495), M491 (= M497), V492 (= V498), W495 (= W501), L517 (= L524), G522 (= G529), L523 (≠ A530), K556 (≠ P563)
binding methyl 2-[({[(4-methoxy-6-methyl-1,3,5-triazin-2-yl)amino]carbonyl}amino)sulfonyl]benzoate: G32 (= G52), A33 (= A53), V107 (= V126), P108 (= P127), F117 (= F136), K167 (= K186), M263 (= M281), D288 (= D306), R289 (= R307), W495 (= W501)
binding flavin-adenine dinucleotide: R157 (= R176), G216 (= G235), A217 (≠ G236), G218 (= G237), N221 (≠ K240), T243 (= T261), L244 (≠ M262), Q245 (≠ P263), M260 (≠ W278), L261 (≠ A279), H264 (= H282), G283 (= G301), A284 (≠ T302), R285 (= R303), D287 (= D305), R289 (= R307), V290 (≠ L308), E316 (≠ D325), V317 (≠ I326), N321 (≠ E330), G334 (= G343), D335 (= D344), A336 (= A345), Q410 (= Q415), M411 (= M416), G429 (≠ H434), G430 (= G435)
binding magnesium ion: D459 (= D465), N486 (= N492), E488 (≠ Y494)
binding 2,5-dimethyl-pyrimidin-4-ylamine: E55 (= E74), P81 (= P100), Q118 (= Q137), G432 (= G437), M434 (= M439), M464 (= M470)

Query Sequence

>WP_004043427.1 NCBI__GCF_000337315.1:WP_004043427.1
MSERTAATTDPDATDAADDAAQTPTEVTSGSTAVVRALENAGVETAFGVQGGAIMPIYDA
LYHSDIRHVTMAHEQGAAHAADAYGVVRGDPGVCLATSGPGATNLVTGIADANMDSDAVL
ALTGQVPSDMVGSDAFQETDTTGVTAPITKHNYFASSPDTVGDTVGEAFALAAEGRPGPT
LVDLPKDVSLNETDTESGPAQPPETCRARTDPADSQVEAAARAIERAEKPLLLFGGGVVK
ADATEVARQFATEHRIPVVTTMPGIGAMPEDHELCLSWAGMHGTGYANMAITHTDCLIAV
GTRFDDRLTGGIDTFAPEAEVVHIDIDPAEISKNVHADYPVVGDAGRAIERVDAELDEGP
DAREWVDQCLAWKDDYPMDYAAPDNEPLKPQFVVEALDEATPDETIVTSGVGQHQMWAAQ
YWTFTEPRRWVSSHGLGTMGYGLPAAIGARVAADDDETVVCVDGDASFLMTIQELSVAVR
ENLDITVAILNNEYIGMVRQWQDAFFEGRRMAAGYDWCPQFDKLAEAFGARGWTVDSYEE
VPDVIEEALAYDGPSVIDFHVDPAENVYPMVSSGGANGKFALSEDQL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory