SitesBLAST
Comparing WP_004043628.1 NCBI__GCF_000337315.1:WP_004043628.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
3nvsA 1.02 angstrom resolution crystal structure of 3-phosphoshikimate 1- carboxyvinyltransferase from vibrio cholerae in complex with shikimate-3-phosphate (partially photolyzed) and glyphosate
35% identity, 96% coverage: 10:423/430 of query aligns to 12:424/426 of 3nvsA
- active site: K22 (= K20), S23 (= S21), D49 (= D46), N94 (= N88), P119 (≠ A113), R124 (= R118), H128 (≠ P122), Q135 (≠ D129), Y142 (≠ S136), E144 (≠ R138), A247 (≠ L240), A255 (= A248), D314 (= D311), E342 (= E339), H386 (= H384), R387 (= R385), K412 (≠ V411)
- binding glyphosate: K22 (= K20), G96 (= G90), R124 (= R118), Q172 (= Q165), D314 (= D311), E342 (= E339), R345 (= R342), H386 (= H384), R387 (= R385)
- binding magnesium ion: E123 (≠ S117), Q145 (≠ R139)
- binding shikimate-3-phosphate: K22 (= K20), S23 (= S21), R27 (= R25), T97 (= T91), S170 (= S163), S171 (= S164), Q172 (= Q165), S198 (= S191), Y201 (= Y194), D314 (= D311), N337 (≠ H334), K341 (= K338)
- binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: S23 (= S21), R27 (= R25), Q172 (= Q165), Y201 (= Y194), D314 (= D311), K341 (= K338)
Q9KRB0 3-phosphoshikimate 1-carboxyvinyltransferase; 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EPSPS; EC 2.5.1.19 from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
35% identity, 96% coverage: 10:423/430 of query aligns to 12:424/426 of Q9KRB0
- K22 (= K20) binding 3-phosphoshikimate
- S23 (= S21) binding 3-phosphoshikimate
- R27 (= R25) binding 3-phosphoshikimate
- S170 (= S163) binding 3-phosphoshikimate
- S171 (= S164) binding 3-phosphoshikimate
- S198 (= S191) binding 3-phosphoshikimate
- D314 (= D311) binding 3-phosphoshikimate
- N337 (≠ H334) binding 3-phosphoshikimate
- K341 (= K338) binding 3-phosphoshikimate
2pq9A E. Coli epsps liganded with (r)-difluoromethyl tetrahedral reaction intermediate analog (see paper)
36% identity, 96% coverage: 8:420/430 of query aligns to 10:420/427 of 2pq9A
- active site: K22 (= K20), S23 (= S21), D49 (= D46), N94 (= N88), P119 (≠ A113), R124 (= R118), D313 (= D311), E341 (= E339), H385 (= H384), R386 (= R385), K411 (≠ V411)
- binding (3r,4s,5r)-5-[(1r)-1-carboxy-2,2-difluoro-1-(phosphonooxy)ethoxy]-4-hydroxy-3-(phosphonooxy)cyclohex-1-ene-1-carboxylic acid: K22 (= K20), S23 (= S21), R27 (= R25), G96 (= G90), T97 (= T91), R124 (= R118), S169 (= S163), S170 (= S164), Q171 (= Q165), S197 (= S191), Y200 (= Y194), D313 (= D311), N336 (≠ H334), K340 (= K338), R344 (= R342), H385 (= H384), R386 (= R385), K411 (≠ V411)
2aa9A Epsp synthase liganded with shikimate (see paper)
36% identity, 96% coverage: 8:420/430 of query aligns to 10:420/427 of 2aa9A
- active site: K22 (= K20), S23 (= S21), D49 (= D46), N94 (= N88), P119 (≠ A113), R124 (= R118), D313 (= D311), E341 (= E339), H385 (= H384), R386 (= R385), K411 (≠ V411)
- binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: K22 (= K20), S23 (= S21), R27 (= R25), T97 (= T91), Q171 (= Q165), Y200 (= Y194), D313 (= D311), K340 (= K338)
1x8tA Epsps liganded with the (r)-phosphonate analog of the tetrahedral reaction intermediate (see paper)
36% identity, 96% coverage: 8:420/430 of query aligns to 10:420/427 of 1x8tA
- active site: K22 (= K20), S23 (= S21), D49 (= D46), N94 (= N88), P119 (≠ A113), R124 (= R118), D313 (= D311), E341 (= E339), H385 (= H384), R386 (= R385), K411 (≠ V411)
- binding [3r-[3a,4a,5b(r*)]]-5-(1-carboxy-1-phosphonoethoxy)-4-hydroxy-3-(phosphonooxy)-1-cyclohexene-1-carboxylic acid: K22 (= K20), S23 (= S21), R27 (= R25), T97 (= T91), S169 (= S163), S170 (= S164), Q171 (= Q165), S197 (= S191), Y200 (= Y194), D313 (= D311), N336 (≠ H334), K340 (= K338), R344 (= R342), H385 (= H384), R386 (= R385)
1x8rA Epsps liganded with the (s)-phosphonate analog of the tetrahedral reaction intermediate (see paper)
36% identity, 96% coverage: 8:420/430 of query aligns to 10:420/427 of 1x8rA
- active site: K22 (= K20), S23 (= S21), D49 (= D46), N94 (= N88), P119 (≠ A113), R124 (= R118), D313 (= D311), E341 (= E339), H385 (= H384), R386 (= R385), K411 (≠ V411)
- binding [3r-[3a,4a,5b(s*)]]-5-(1-carboxy-1-phosphonoethoxy)-4-hydroxy-3-(phosphonooxy)-1-cyclohexene-1-carboxylic acid: K22 (= K20), S23 (= S21), R27 (= R25), G96 (= G90), T97 (= T91), R124 (= R118), S169 (= S163), S170 (= S164), Q171 (= Q165), S197 (= S191), Y200 (= Y194), D313 (= D311), N336 (≠ H334), K340 (= K338), E341 (= E339), H385 (= H384), K411 (≠ V411)
1g6tA Structure of epsp synthase liganded with shikimate-3-phosphate (see paper)
36% identity, 96% coverage: 8:420/430 of query aligns to 10:420/427 of 1g6tA
- active site: K22 (= K20), S23 (= S21), D49 (= D46), N94 (= N88), P119 (≠ A113), R124 (= R118), D313 (= D311), E341 (= E339), H385 (= H384), R386 (= R385), K411 (≠ V411)
- binding phosphate ion: K22 (= K20), G96 (= G90), T97 (= T91), R124 (= R118), Q171 (= Q165), E341 (= E339), K411 (≠ V411)
- binding shikimate-3-phosphate: K22 (= K20), S23 (= S21), R27 (= R25), T97 (= T91), S169 (= S163), S170 (= S164), Q171 (= Q165), S197 (= S191), Y200 (= Y194), D313 (= D311), N336 (≠ H334), K340 (= K338)
1g6sA Structure of epsp synthase liganded with shikimate-3-phosphate and glyphosate (see paper)
36% identity, 96% coverage: 8:420/430 of query aligns to 10:420/427 of 1g6sA
- active site: K22 (= K20), S23 (= S21), D49 (= D46), N94 (= N88), P119 (≠ A113), R124 (= R118), D313 (= D311), E341 (= E339), H385 (= H384), R386 (= R385), K411 (≠ V411)
- binding glyphosate: K22 (= K20), G96 (= G90), R124 (= R118), Q171 (= Q165), D313 (= D311), E341 (= E339), R344 (= R342), H385 (= H384), R386 (= R385)
- binding shikimate-3-phosphate: K22 (= K20), S23 (= S21), R27 (= R25), T97 (= T91), S169 (= S163), S170 (= S164), Q171 (= Q165), S197 (= S191), Y200 (= Y194), D313 (= D311), N336 (≠ H334), K340 (= K338)
P0A6D3 3-phosphoshikimate 1-carboxyvinyltransferase; 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EPSPS; EC 2.5.1.19 from Escherichia coli (strain K12) (see 5 papers)
36% identity, 96% coverage: 8:420/430 of query aligns to 10:420/427 of P0A6D3
- K22 (= K20) binding 3-phosphoshikimate
- S23 (= S21) binding 3-phosphoshikimate
- R27 (= R25) binding 3-phosphoshikimate
- G96 (= G90) mutation to A: Insensitive to glyphosate with unaltered affinity for its first substrate S3P, but displays a 30-fold lower affinity for its second substrate PEP.
- T97 (= T91) mutation to I: This mutant is sensitive to glyphosate and causes a substantial decrease in the affinity for PEP. Is insensitive to glyphosate but maintains high affinity for PEP; when associated with S-101.
- P101 (≠ L95) mutation to A: Displays a slight decrease of the affinity binding for both S3P and PEP. Decreases the binding affinity of glyphosate, reducing the potency of this inhibitor.; mutation to G: Displays a slight decrease of the affinity binding for both S3P and PEP. Decreases the binding affinity of glyphosate, reducing the potency of this inhibitor.; mutation to L: Displays a 2-fold lower affinity binding for both S3P and PEP. Decreases the binding affinity of glyphosate, reducing the potency of this inhibitor.; mutation to S: Displays a slight decrease of the affinity binding for both S3P and PEP. Decreases the binding affinity of glyphosate, reducing the potency of this inhibitor. Is insensitive to glyphosate but maintains high affinity for PEP; when associated with I-97.
- S169 (= S163) binding 3-phosphoshikimate
- S170 (= S164) binding 3-phosphoshikimate
- Q171 (= Q165) binding 3-phosphoshikimate
- S197 (= S191) binding 3-phosphoshikimate
- D313 (= D311) binding 3-phosphoshikimate; mutation to A: The enolpyruvyl transfer reaction is halted after formation of the tetrahedral adduct of the substrates.
- N336 (≠ H334) binding 3-phosphoshikimate
- K340 (= K338) binding 3-phosphoshikimate
- C408 (≠ H408) Modified by bromopyruvate
- K411 (≠ V411) Modified by bromopyruvate
3fjzA E. Coli epsp synthase (t97i) liganded with s3p and glyphosate (see paper)
36% identity, 96% coverage: 8:420/430 of query aligns to 10:420/427 of 3fjzA
- active site: K22 (= K20), S23 (= S21), D49 (= D46), N94 (= N88), P119 (≠ A113), R124 (= R118), D313 (= D311), E341 (= E339), H385 (= H384), R386 (= R385), K411 (≠ V411)
- binding N-(phosphonomethyl)glycine: K22 (= K20), G96 (= G90), R124 (= R118), Q171 (= Q165), D313 (= D311), E341 (= E339), R344 (= R342), H385 (= H384), R386 (= R385)
- binding shikimate-3-phosphate: K22 (= K20), S23 (= S21), R27 (= R25), I97 (≠ T91), S169 (= S163), S170 (= S164), Q171 (= Q165), S197 (= S191), Y200 (= Y194), D313 (= D311), N336 (≠ H334), K340 (= K338)
- binding serine: I265 (≠ G261), G266 (≠ A262), S269 (= S265)
7tm5B Crystal structure of shikimate-3-phosphate bound 3-phosphoshikimate 1- carboxyvinyltransferase from klebsiella pneumoniae
36% identity, 96% coverage: 8:420/430 of query aligns to 10:420/427 of 7tm5B
- binding shikimate-3-phosphate: K22 (= K20), S23 (= S21), R27 (= R25), S169 (= S163), S170 (= S164), Q171 (= Q165), S197 (= S191), Y200 (= Y194), D313 (= D311), N336 (≠ H334), K340 (= K338)
7tm6A Crystal structure of shikimate-3-phosphate and glyphosate bound 3- phosphoshikimate 1-carboxyvinyltransferase from klebsiella pneumoniae
36% identity, 96% coverage: 8:420/430 of query aligns to 9:419/426 of 7tm6A
- binding glyphosate: K21 (= K20), G95 (= G90), R123 (= R118), Q170 (= Q165), D312 (= D311), E340 (= E339), R343 (= R342), H384 (= H384), R385 (= R385)
- binding shikimate-3-phosphate: S22 (= S21), R26 (= R25), T96 (= T91), S168 (= S163), S169 (= S164), Q170 (= Q165), S196 (= S191), Y199 (= Y194), D312 (= D311), N335 (≠ H334), K339 (= K338)
1q36A Epsp synthase (asp313ala) liganded with tetrahedral reaction intermediate (see paper)
36% identity, 96% coverage: 8:420/430 of query aligns to 10:420/427 of 1q36A
- active site: K22 (= K20), S23 (= S21), D49 (= D46), N94 (= N88), P119 (≠ A113), R124 (= R118), A313 (≠ D311), E341 (= E339), H385 (= H384), R386 (= R385), K411 (≠ V411)
- binding 5-(1-carboxy-1-phosphonooxy-ethoxyl)-4-hydroxy-3-phosphonooxy-cyclohex-1-enecarboxylic acid: K22 (= K20), S23 (= S21), R27 (= R25), G96 (= G90), T97 (= T91), R124 (= R118), S169 (= S163), S170 (= S164), Q171 (= Q165), S197 (= S191), Y200 (= Y194), N336 (≠ H334), K340 (= K338), E341 (= E339), R344 (= R342), R386 (= R385), K411 (≠ V411)
P11043 3-phosphoshikimate 1-carboxyvinyltransferase, chloroplastic; 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EC 2.5.1.19 from Petunia hybrida (Petunia) (see paper)
33% identity, 96% coverage: 10:422/430 of query aligns to 85:512/516 of P11043
- G173 (= G90) mutation to A: This mutant becomes resistant to glyphosate due to a lower affinity. Shows a slight reduction in EPSP synthase activity.
Q83E11 3-phosphoshikimate 1-carboxyvinyltransferase; 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EPSPS; EC 2.5.1.19 from Coxiella burnetii (strain RSA 493 / Nine Mile phase I)
30% identity, 99% coverage: 1:424/430 of query aligns to 1:426/438 of Q83E11
- K21 (= K20) binding phosphoenolpyruvate
- S22 (= S21) binding 3-phosphoshikimate
- R26 (= R25) binding 3-phosphoshikimate
- NSGT 93:96 (= NSGT 88:91) Phosphoenolpyruvate
- G95 (= G90) binding phosphoenolpyruvate
- T96 (= T91) binding phosphoenolpyruvate
- R123 (= R118) binding phosphoenolpyruvate
- S167 (= S163) binding 3-phosphoshikimate
- A168 (≠ S164) binding 3-phosphoshikimate
- Q169 (= Q165) binding 3-phosphoshikimate; binding phosphoenolpyruvate
- D315 (= D311) binding 3-phosphoshikimate
- K342 (= K338) binding 3-phosphoshikimate
- R346 (= R342) binding phosphoenolpyruvate
- R387 (= R385) binding phosphoenolpyruvate
3slhD 1.70 angstrom resolution structure of 3-phosphoshikimate 1- carboxyvinyltransferase (aroa) from coxiella burnetii in complex with shikimate-3-phosphate and glyphosate
30% identity, 99% coverage: 1:424/430 of query aligns to 3:428/440 of 3slhD
- active site: K23 (= K20), S24 (= S21), D50 (= D46), N95 (= N88), R125 (= R118), D317 (= D311), E345 (= E339), H388 (= H384), R389 (= R385), T415 (≠ V411)
- binding glyphosate: K23 (= K20), G97 (= G90), T98 (= T91), R125 (= R118), Q171 (= Q165), D317 (= D311), E345 (= E339), R348 (= R342), H388 (= H384), R389 (= R385)
- binding shikimate-3-phosphate: S24 (= S21), R28 (= R25), S169 (= S163), Q171 (= Q165), R196 (≠ V195), D317 (= D311), K344 (= K338)
- binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: S24 (= S21), R28 (= R25), T98 (= T91), Q171 (= Q165), R196 (≠ V195), D317 (= D311), K344 (= K338)
4egrA 2.50 angstrom resolution structure of 3-phosphoshikimate 1- carboxyvinyltransferase (aroa) from coxiella burnetii in complex with phosphoenolpyruvate
30% identity, 99% coverage: 1:424/430 of query aligns to 3:424/434 of 4egrA
- active site: K23 (= K20), S24 (= S21), D50 (= D46), N95 (= N88), R125 (= R118), D313 (= D311), E341 (= E339), H384 (= H384), R385 (= R385), T411 (≠ V411)
- binding phosphoenolpyruvate: K23 (= K20), G97 (= G90), T98 (= T91), R125 (= R118), D313 (= D311), E341 (= E339), R344 (= R342), R385 (= R385)
7m0oA Dgt-28 epsps (see paper)
36% identity, 83% coverage: 65:423/430 of query aligns to 49:397/400 of 7m0oA
P07547 Pentafunctional AROM polypeptide; EC 4.2.3.4; EC 2.5.1.19; EC 2.7.1.71; EC 4.2.1.10; EC 1.1.1.25 from Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) (see 2 papers)
31% identity, 95% coverage: 16:423/430 of query aligns to 410:839/1583 of P07547
Sites not aligning to the query:
- 44:46 binding NAD(+)
- 81:84 binding NAD(+)
- 114:116 binding NAD(+)
- 119 binding NAD(+)
- 139:140 binding NAD(+)
- 161 binding NAD(+)
- 179:182 binding NAD(+)
- 190 binding NAD(+)
- 194 binding Zn(2+)
- 271 binding Zn(2+)
- 287 binding Zn(2+)
Q9S400 3-phosphoshikimate 1-carboxyvinyltransferase; 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EPSPS; EC 2.5.1.19 from Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) (see paper)
31% identity, 98% coverage: 1:423/430 of query aligns to 1:424/427 of Q9S400
- S21 (= S21) binding 3-phosphoshikimate
- R25 (= R25) binding 3-phosphoshikimate
- S166 (= S164) binding 3-phosphoshikimate
- A167 (≠ Q165) binding 3-phosphoshikimate
- Q168 (≠ F166) binding 3-phosphoshikimate
- D312 (vs. gap) binding 3-phosphoshikimate
- K339 (= K338) binding 3-phosphoshikimate
Query Sequence
>WP_004043628.1 NCBI__GCF_000337315.1:WP_004043628.1
MDAHVTPSRVAGRTRAPPSKSYTHRAVLAAGYSDEAVVRDALVSADTKATMRAVEAFGGS
VDCDGQTVEVAGFGGRPGTPDDVVNCENSGTTMRLVTGCAALGEGLTVLTGDASLRSRPH
GPLLDAVFDLDGRAESTRRNGQAPLVVGDGMTGGTVEIPGDVSSQFITALLMAGAVTEEG
IDVELTTELKSSPYVDITLELLADFGVEATRTDAGFSVPGGQSYAPEGGAYSVPGDFSSL
SYLLAAGAVAGAEGEDVVIEGARPSAQGDSAIVDIVRDMGATVDWDEDAGELVVSAADLT
GTTVDVGDTPDLLPTIAVLGAIADGDTVIENCEHVRFKETDRVSAMAEELAEMGANVTEQ
RDRLTIHGGETDLVGAAVDGRGDHRIVMSLAVAGLVADGETTIAGSEHVDVSFPNFFEAM
RDLGAEMTEH
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory