SitesBLAST
Comparing WP_004044594.1 NCBI__GCF_000337315.1:WP_004044594.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
4whxA X-ray crystal structure of an amino acid aminotransferase from burkholderia pseudomallei bound to the co-factor pyridoxal phosphate
55% identity, 96% coverage: 11:309/312 of query aligns to 9:306/306 of 4whxA
1iyeA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase (see paper)
51% identity, 96% coverage: 11:310/312 of query aligns to 5:304/304 of 1iyeA
- active site: F33 (= F39), G35 (= G41), K156 (= K161), A157 (≠ T162), E190 (= E195), L214 (= L219)
- binding N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-L-glutamic acid: R56 (= R62), Y92 (= Y98), Y126 (= Y131), K156 (= K161), Y161 (= Y166), E190 (= E195), G193 (= G198), E194 (= E199), N195 (= N200), L214 (= L219), G216 (= G221), I217 (= I222), T218 (= T223), G253 (= G259), T254 (≠ S260), A255 (= A261)
1iydA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase (see paper)
51% identity, 96% coverage: 11:310/312 of query aligns to 5:304/304 of 1iydA
- active site: F33 (= F39), G35 (= G41), K156 (= K161), A157 (≠ T162), E190 (= E195), L214 (= L219)
- binding glutaric acid: Y92 (= Y98), Y126 (= Y131), A255 (= A261)
- binding pyridoxal-5'-phosphate: R56 (= R62), K156 (= K161), Y161 (= Y166), E190 (= E195), G193 (= G198), E194 (= E199), L214 (= L219), G216 (= G221), I217 (= I222), T218 (= T223), T254 (≠ S260)
1i1mA Crystal structure of escherichia coli branched-chain amino acid aminotransferase. (see paper)
51% identity, 96% coverage: 11:310/312 of query aligns to 5:304/304 of 1i1mA
- active site: K156 (= K161)
- binding 4-methyl valeric acid: Y92 (= Y98), K156 (= K161), T254 (≠ S260), A255 (= A261)
- binding pyridoxal-5'-phosphate: R56 (= R62), K156 (= K161), Y161 (= Y166), E190 (= E195), G193 (= G198), E194 (= E199), L214 (= L219), G216 (= G221), I217 (= I222), T218 (= T223), G253 (= G259), T254 (≠ S260)
1i1lA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase. (see paper)
51% identity, 96% coverage: 11:310/312 of query aligns to 5:304/304 of 1i1lA
- active site: K156 (= K161)
- binding 2-methylleucine: Y92 (= Y98), K156 (= K161), T254 (≠ S260), A255 (= A261)
- binding pyridoxal-5'-phosphate: R56 (= R62), K156 (= K161), Y161 (= Y166), E190 (= E195), G193 (= G198), G216 (= G221), I217 (= I222), T218 (= T223), T254 (≠ S260)
2ej0B Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase with pyridoxamine 5'-phosphate
50% identity, 98% coverage: 5:310/312 of query aligns to 1:305/305 of 2ej0B
- active site: F35 (= F39), G37 (= G41), K158 (= K161), E192 (= E195), L215 (= L219)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: R58 (= R62), Y163 (= Y166), E192 (= E195), G195 (= G198), E196 (= E199), L215 (= L219), G217 (= G221), I218 (= I222), T219 (= T223), G254 (= G259), T255 (≠ S260)
2ej3A Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase complexed with gabapentin
48% identity, 98% coverage: 5:310/312 of query aligns to 1:297/297 of 2ej3A
- active site: F35 (= F39), G37 (= G41), K150 (= K161), E184 (= E195), L207 (= L219)
- binding [1-(aminomethyl)cyclohexyl]acetic acid: G187 (= G198), G246 (= G259), T247 (≠ S260), A248 (= A261)
- binding pyridoxal-5'-phosphate: R58 (= R62), K150 (= K161), Y155 (= Y166), E184 (= E195), G187 (= G198), L207 (= L219), G209 (= G221), I210 (= I222), T211 (= T223), G246 (= G259), T247 (≠ S260)
2eiyA Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase complexed with 4-methylvaleric acid
48% identity, 98% coverage: 5:310/312 of query aligns to 1:297/297 of 2eiyA
- active site: F35 (= F39), G37 (= G41), K150 (= K161), E184 (= E195), L207 (= L219)
- binding 4-methyl valeric acid: F35 (= F39), Y94 (= Y98), T247 (≠ S260), A248 (= A261)
- binding pyridoxal-5'-phosphate: R58 (= R62), K150 (= K161), Y155 (= Y166), E184 (= E195), G187 (= G198), E188 (= E199), L207 (= L219), G209 (= G221), I210 (= I222), T211 (= T223), G246 (= G259), T247 (≠ S260)
1wrvA Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase
48% identity, 98% coverage: 5:310/312 of query aligns to 1:297/297 of 1wrvA
- active site: F35 (= F39), G37 (= G41), K150 (= K161), E184 (= E195), L207 (= L219)
- binding pyridoxal-5'-phosphate: R58 (= R62), K150 (= K161), Y155 (= Y166), E184 (= E195), G187 (= G198), L207 (= L219), G209 (= G221), I210 (= I222), T211 (= T223), T247 (≠ S260)
2ej2A Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase complexed with n-(5'-phosphopyridoxyl)-l-glutamate
48% identity, 98% coverage: 5:310/312 of query aligns to 1:294/294 of 2ej2A
- active site: F35 (= F39), G37 (= G41), K147 (= K161), E181 (= E195), L204 (= L219)
- binding 4-[(1,3-dicarboxy-propylamino)-methyl]-3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridinium: R58 (= R62), Y94 (= Y98), Y152 (= Y166), E181 (= E195), G184 (= G198), E185 (= E199), L204 (= L219), G206 (= G221), I207 (= I222), T208 (= T223), T244 (≠ S260), A245 (= A261)
6thqB Crystal structure of branched-chain aminotransferase from thermophilic archaea thermoproteus uzoniensis with norvaline
44% identity, 96% coverage: 11:309/312 of query aligns to 9:300/301 of 6thqB
- active site: F37 (= F39), K156 (= K161), E190 (= E195), L214 (= L219)
- binding pyridoxal-5'-phosphate: R60 (= R62), K156 (= K161), Y161 (= Y166), E190 (= E195), N195 (= N200), L214 (= L219), G216 (= G221), I217 (= I222), T218 (= T223), T254 (≠ S260)
- binding 2-[o-phosphonopyridoxyl]-amino-pentanoic acid: R60 (= R62), Y97 (= Y98), K156 (= K161), Y161 (= Y166), E190 (= E195), G193 (= G198), E194 (= E199), N195 (= N200), G216 (= G221), I217 (= I222), T218 (= T223), G253 (= G259), T254 (≠ S260), A255 (= A261)
5mr0D Thermophilic archaeal branched-chain amino acid transaminases from geoglobus acetivorans and archaeoglobus fulgidus: biochemical and structural characterisation (see paper)
41% identity, 92% coverage: 11:296/312 of query aligns to 4:282/290 of 5mr0D
- active site: F32 (= F39), G34 (= G41), K150 (= K161), E183 (= E195), L206 (= L219)
- binding 3-[o-phosphonopyridoxyl]--amino-benzoic acid: R51 (= R62), G100 (= G111), L101 (≠ V112), K150 (= K161), Y154 (= Y166), E183 (= E195), G186 (= G198), D187 (≠ E199), L206 (= L219), I209 (= I222), T210 (= T223), G245 (= G259), T246 (≠ S260)
5e25A Crystal structure of branched-chain aminotransferase from thermophilic archaea geoglobus acetivorans complexed with alpha-ketoglutarate (see paper)
40% identity, 90% coverage: 11:291/312 of query aligns to 5:278/290 of 5e25A
- active site: F33 (= F39), G35 (= G41), K151 (= K161), E184 (= E195), L207 (= L219)
- binding 2-oxoglutaric acid: Y88 (= Y98), K151 (= K161), T247 (≠ S260), A248 (= A261)
- binding pyridoxal-5'-phosphate: R52 (= R62), K151 (= K161), Y155 (= Y166), E184 (= E195), G187 (= G198), D188 (≠ E199), L207 (= L219), G209 (= G221), I210 (= I222), T211 (= T223), G246 (= G259), T247 (≠ S260)
6q8eA Crystal structure of branched-chain amino acid aminotransferase from thermobaculum terrenum in pmp-form (see paper)
38% identity, 96% coverage: 11:309/312 of query aligns to 6:304/307 of 6q8eA
- active site: F34 (= F39), K156 (= K161), E190 (= E195), L214 (= L219)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: R59 (= R62), K156 (= K161), Y161 (= Y166), E190 (= E195), G193 (= G198), S194 (≠ E199), C195 (≠ N200), L214 (= L219), S216 (≠ G221), I217 (= I222), T218 (= T223), G254 (= G259), T255 (≠ S260)
6h65C Crystal structure of the branched-chain-amino-acid aminotransferase from haliangium ochraceum
32% identity, 96% coverage: 12:311/312 of query aligns to 8:308/308 of 6h65C
- active site: F35 (= F39), K158 (= K161), E192 (= E195), L216 (= L219)
- binding pyridoxal-5'-phosphate: R60 (= R62), K158 (= K161), Y163 (= Y166), E192 (= E195), A196 (≠ E199), L216 (= L219), S218 (≠ G221), V219 (≠ I222), T220 (= T223), G256 (= G259), T257 (≠ S260)
7neaA Crystal structure of branched-chain amino acid aminotransferase from thermobaculum terrenum (m3 mutant). (see paper)
37% identity, 96% coverage: 11:309/312 of query aligns to 6:304/309 of 7neaA
- active site: F34 (= F39), K156 (= K161), E190 (= E195), L214 (= L219)
- binding pyridoxal-5'-phosphate: R59 (= R62), K156 (= K161), Y161 (= Y166), E190 (= E195), G193 (= G198), S194 (≠ E199), L214 (= L219), S216 (≠ G221), I217 (= I222), T218 (= T223), T255 (≠ S260)
7p3tB Transaminase of gamma-proteobacterium (see paper)
31% identity, 93% coverage: 7:296/312 of query aligns to 2:284/299 of 7p3tB
- binding pyridoxal-5'-phosphate: R53 (= R62), K153 (= K161), R157 (≠ Y166), E186 (= E195), S187 (≠ G196), A188 (≠ P197), A189 (≠ G198), S190 (≠ E199), G210 (= G221), I211 (= I222), T212 (= T223), T248 (≠ S260)
P19938 D-alanine aminotransferase; D-amino acid aminotransferase; D-amino acid transaminase; DAAT; D-aspartate aminotransferase; EC 2.6.1.21 from Bacillus sp. (strain YM-1) (see 5 papers)
31% identity, 90% coverage: 10:291/312 of query aligns to 4:273/283 of P19938
- Y32 (≠ F39) binding substrate
- R51 (= R62) binding pyridoxal 5'-phosphate
- R99 (≠ K115) binding substrate
- H101 (vs. gap) binding substrate
- K146 (= K161) active site, Proton acceptor; modified: N6-(pyridoxal phosphate)lysine
- E178 (= E195) binding pyridoxal 5'-phosphate; mutation to K: Loss of transaminase activity and small gain in racemase activity.
- L202 (= L219) mutation to A: Inactivates enzyme.
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
3lqsA Complex structure of d-amino acid aminotransferase and 4-amino-4,5- dihydro-thiophenecarboxylic acid (adta) (see paper)
31% identity, 90% coverage: 10:291/312 of query aligns to 3:272/280 of 3lqsA
- active site: Y31 (≠ F39), V33 (≠ G41), K145 (= K161), E177 (= E195), L201 (= L219)
- binding 4-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]thiophene-2-carboxylic acid: V33 (≠ G41), R50 (= R62), E177 (= E195), S180 (≠ G198), S181 (≠ E199), N182 (= N200), L201 (= L219), G203 (= G221), I204 (= I222), T205 (= T223), S240 (≠ G259), T241 (≠ S260), T242 (≠ A261)
3daaA Crystallographic structure of d-amino acid aminotransferase inactivated by pyridoxyl-d-alanine (see paper)
31% identity, 90% coverage: 10:291/312 of query aligns to 3:272/277 of 3daaA
- active site: Y31 (≠ F39), V33 (≠ G41), K145 (= K161), E177 (= E195), L201 (= L219)
- binding n-(5'-phosphopyridoxyl)-d-alanine: Y31 (≠ F39), R50 (= R62), K145 (= K161), E177 (= E195), S180 (≠ G198), S181 (≠ E199), L201 (= L219), G203 (= G221), I204 (= I222), T205 (= T223), S240 (≠ G259), T241 (≠ S260)
Query Sequence
>WP_004044594.1 NCBI__GCF_000337315.1:WP_004044594.1
MGFDEMDVSTIWQSGEYKDWEDATVHVLTHGLHYGTGVFEGVRAYDTDRGPAIFRWEEHL
ERLYQSAKPYDMEIPYTREELTEATLEVIRRNDLDGGYIRPIAFYGYDSLGVSPKDNPTE
VAIAAWPWGTYLGEDALENGVDVMVSSWRKHASSQIPTNAKTTGLYVNSMLAGEEARRNG
YVEAIVLNKEGNVAEGPGENIFMVRDGKIYTPGLSQSILDGITRDTVITLARERGYEVDD
SATISRGELNTADELFFTGSAAEVTPIRKVDNVVIGEGTRGPVTEELQSAFFDLVERRTD
DHEEWFTYVDEQ
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory