SitesBLAST

P00903 Aminodeoxychorismate synthase component 2; ADC synthase; ADCS; 4-amino-4-deoxychorismate synthase component 2; Aminodeoxychorismate synthase, glutamine amidotransferase component; EC 2.6.1.85 from Escherichia coli (strain K12) (see paper)
35% identity, 41% coverage: 80:175/237 of query aligns to 73:170/187 of P00903

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P00903

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C79 (= C86) mutation to S: 10000-fold decrease in catalytic efficiency.
H168 (= H173) mutation to Q: Loss of activity.
E170 (= E175) mutation to A: 150-fold decrease in catalytic efficiency.; mutation to D: 4-fold decrease in catalytic efficiency.; mutation E->K,Q: Loss of activity.

P00900 Anthranilate synthase component 2; AS; ASII; Anthranilate synthase, GATase component; Anthranilate synthase, glutamine amidotransferase component; EC 4.1.3.27 from Serratia marcescens (see 3 papers)
30% identity, 43% coverage: 80:180/237 of query aligns to 78:177/193 of P00900

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P00900

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C84 (= C86) active site, Nucleophile; for GATase activity

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

Q18990 Multifunctional protein pyr-1; EC 6.3.5.5; EC 3.5.1.2; EC 6.3.4.16; EC 2.1.3.2; EC 3.5.2.3 from Caenorhabditis elegans (see 3 papers)
27% identity, 56% coverage: 45:176/237 of query aligns to 217:342/2198 of Q18990

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Q18990

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Sites not aligning to the query:

1602 H→Q: In cu8; probable loss of dihydroorotase activity. Severe late stage embryonic lethality in 50% of animals. The few surviving mutants have a shorter and thicker pharyngeal isthmus, an abnormal knobbed tail, mild egg-laying defects, moderate fluid accumulation in the coelom and a slower growth. Actin and intermediate filaments are disorganized in the pharynx. Moderate reduction in heparan sulfate levels and increased levels of chondroitin sulfate. In an umps-1 zu456 mutant background, prevents the formation of abnormally enlarged gut granules in embryos. Complete embryonic lethality in a rnst-2 qx245 mutant background.

P08955 Multifunctional protein CAD; Carbamoyl phosphate synthetase 2-aspartate transcarbamylase-dihydroorotase; EC 6.3.5.5; EC 3.5.1.2; EC 6.3.4.16; EC 2.1.3.2; EC 3.5.2.3 from Mesocricetus auratus (Golden hamster) (see paper)
29% identity, 57% coverage: 42:176/237 of query aligns to 210:339/2225 of P08955

query
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P08955

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Sites not aligning to the query:

1406 modified: Phosphoserine; by PKA; S→A: No effect on enzyme kinetics.; S→D: Increases CPSase activity and reduces sensitivity to feedback inhibition by UTP.

Query Sequence

>WP_004045322.1 NCBI__GCF_000337315.1:WP_004045322.1
MTRPRLAFLDASHGDSSTSRNFRRELDADLVEFDVTDGRLPDHFDYDGVVISGSSSSVYW
DEPWIRNLVSWVADADERGVPLLGVCFGHQVVAAALGGTVEDMGAFELGYNEIERTRPDD
GNDILAGIGERFTVFTSHGDRVTELPSGADLLAENEFGVHAFRRNHAFGVQFHPEYDTDT
AEAIARQKDFLPDERIRSVVDGITPENYAAACEAKRLFDNFVTYVNRTNAGSVESAA

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SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory