SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_004109665.1 NCBI__GCF_000359745.1:WP_004109665.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

7qh2C Cryo-em structure of ldh-etfab complex from acetobacterium woodii (see paper)
31% identity, 93% coverage: 26:470/479 of query aligns to 9:463/467 of 7qh2C

query
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binding flavin-adenine dinucleotide: V73 (≠ P84), G75 (= G86), S76 (≠ A87), G77 (= G88), T78 (= T89), G79 (≠ S90), L80 (= L91), A83 (≠ G94), C84 (≠ A95), P137 (= P148), G138 (≠ S149), E139 (≠ S150), A142 (= A153), T143 (= T155), G146 (= G158), N147 (= N159), S149 (≠ G161), T150 (≠ M162), A152 (≠ S164), G153 (= G165), E203 (= E214), G204 (= G215), I209 (≠ V220), E422 (= E429), H423 (= H430)
binding fe (iii) ion: H377 (= H385), H384 (= H392), E422 (= E429)

P9WIT1 Uncharacterized FAD-linked oxidoreductase Rv2280; EC 1.-.-.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
31% identity, 96% coverage: 12:471/479 of query aligns to 2:456/459 of P9WIT1

query
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P9WIT1

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K354 (≠ Y364) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

3pm9A Crystal structure of a putative dehydrogenase (rpa1076) from rhodopseudomonas palustris cga009 at 2.57 a resolution
31% identity, 96% coverage: 13:470/479 of query aligns to 5:464/465 of 3pm9A

query
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Q

E

Q

A

Q

L

A

I

F

W

W

K

K

G

L

R

R

K

E

S

E

A

I

F

S

G

P

A

A

T

Q

G

K

R

P

I

E

A

G

D

G

Y

S

I

I

C

K

M

H

D

D

G

I

T

S

V

V

P

P

L

V

S

A

Q

A

L

V

S

P

Y

Q

V

F

L

I

K

E

K

Q

T

A

A

N

E

A

I

A

V

V

H

V

G

A

Y

L

-

I

-

P

G

G

L

A

R

R

V

P

A

V

N

P

V

F

F

G

H

H

A

L

G

G

D

D

G

G

N

N

M

I

H

H

P

-

L

-

I

-

L

-

F

Y

N

N

A

V

N

S

D

Q

P

P

E

V

D

G

A

A

A

D

K

K

A

A

E

E

-

F

-

L

A

A

A

R

G

W

N

H

D

D

I

V

L

S

R

Q

L

V

C

V

V

F

D

E

A

V

-

V

-

L

-

R

-

L

G

G

C

S

L

I

T

S

G

A

E
|
E

H

H

G

G

V

I

G

G

I

V

E

M

K

K

R

R

D

D

L

E

M

L

R

A

H

E

Q

V

Y

K

A

D

K

K

V

T

D

A

L

I

D

E

Q

L

M

M

M

R

A

S

V

I

R

K

A

A

A

L

F

L

D

D

P

P

G

H

W

G

I

I

L

M

N

N

P

P

S

G

K

K

V

V

active site: A149 (≠ S164), L159 (≠ V174)
binding flavin-adenine dinucleotide: P69 (= P84), Q70 (≠ R85), G71 (= G86), G72 (≠ A87), N73 (≠ G88), T74 (= T89), G75 (≠ S90), L76 (= L91), G79 (= G94), Q80 (≠ A95), L91 (= L106), L133 (≠ P148), G134 (≠ S149), A135 (≠ S150), C139 (= C154), T140 (= T155), G142 (= G157), G143 (= G158), S146 (≠ G161), T147 (≠ M162), A149 (≠ S164), G150 (= G165), E200 (= E214), G201 (= G215), I205 (= I219), I206 (≠ V220), E423 (= E429)

8jdtA Crystal structure of mldhd in complex with 2-ketobutanoic acid (see paper)
28% identity, 87% coverage: 56:471/479 of query aligns to 40:455/455 of 8jdtA

query
sites
8jdtA

P

P

L

D

A

A

V

V

A

V

L

W

P

P

R

Q

T

N

T

V

A

D

E

Q

V

V

A

S

A

R

V

V

M

A

K

S

Y

L

C

C

H

Y

R

N

Y

Q

G

G

I

V

P

P

I

I

V

I

P
|
P

R

F

G
|
G

A
x
T

G
|
G

T
|
T

S
x
G

L

V

S

E

G

G

G
|
G

A
x
V

I

C

P

A

Q

V

E

Q

D

G

A

G

V

V

V

C

L

I

G

N

L
|
L

S

T

K

H

M

M

N

D

R

Q

I

I

L

T

D

E

I

L

D

N

Y

T

A

E

N

D

R

F

T

S

A

V

T

V

V

V

Q

E

A

P

G

G

V

V

T

T

N

R

L

K

N

A

V

L

S

N

E

T

S

H

V

L

S

R

A

D

D

S

G

G

F

L

F

W

Y

F

A

P

P

V

D

D

P
|
P

S
x
G

S
x
A

Q

D

L

-

A

-

C

A

T

S

I

L

G

C

G
|
G

N
x
M

I

A

G
x
A

M
x
T

N

G

S
x
A

G
x
S

G

G

A

T

H

N

C

A

L

V

K

R

Y

Y

G

G

V

T

T

M

T

R

N

D

N

N

L

V

L

I

G

N

V

L

K

E

L

V

V

V

L

L

T

P

D

D

G

G

T

R

I

L

D

H

L

T

G

A

G

G

K

R

A

G

L

R

-

H

-

Y

-

R

-

K

D

S

S

A

A

A

G

G

Y

Y

D

N

L

L

L

T

G

G

L

L

V

F

C

V

G

G

S

S

E
|
E

G
|
G

Q

T

L

L

G

G

I

I

V
x
I

T

T

E

S

A

T

T

T

V

L

R

R

L

L

I

H

A

P

K

A

P

P

E

E

G

A

A

T

R

V

P

A

V

A

L

T

F

C

G

A

F

F

D

P

T

S

S

V

E

Q

E

A

A

A

G

V

A

D

C

S

V

T

A

V

D

Q

V

I

I

L

A

Q

A

A

G

A

I

V

I

P

P

V

V

A

A

R

I

I

E

E

F

F

M

L

D

D

K

D

P

V

A

M

I

M

E

D

I

A

C

C

E

N

A

R

F

H

A

S

K

K

A

L

G

N

Y

C

P

P

L

-

D

-

V

V

G

A

A

P

L

T

L

L

I

F

V

L

E

E

V

F

E

H

G

G

S

S

E

Q

A

Q

E

T

M

L

D

A

D

E

M

Q

L

L

A

Q

S

R

I

T

V

E

E

A

I

I

A

T

R

Q

S

D

H

N

S

G

V

G

K

S

T

H

V

F

R

S

E

W

C

A

Q

E

S

K

A

R

T

N

E

E

A

-

L

-

I

L

W

W

K

A

G

A

R
|
R

K

H

S

N

A

A

F
x
W

G

Y

A

A

T

A

G

L

R

A

I

L

A

S

-

P

-

G

-

S

D

K

Y

A

I

Y

C

S

M

T

D

D

G

V

T

C

V

V

P

P

L

I

S

S

Q

R

L

L

S

P

Y

E

V

I

L

L

K

V

K

E

T

T

A

K

E

E

I

E

V

I

H

K

G

A

Y

S

G

K

L

L

R

T

V

G

A

A

N

I

V

V

F

G

H
|
H

A

V

G

G

D

D

G

G

N

N

M

F

H
|
H

P

C

L

I

I

L

L

L

F

V

N

D

A

P

N

D

D

D

P

A

E

E

D

E

A

Q

A

R

K

R

A

V

E

K

A

A

A

F

G

A

N

E

D

N

I

L

L

G

R

R

L

R

C

A

V

L

D

A

A

L

G

G

C

T

L

C

T

T

G

G

E
|
E

H
|
H

G

G

V

I

G

G

I

L

E

G

K

K

R

R

D

Q

L

L

M

L

R

Q

H

E

Q

E

Y

V

A

G

K

P

V

V

D

G

L

V

D

E

Q

T

M

M

M

R

A

Q

V

L

R

K

A

N

A

T

F

L

D

D

P

P

G

R

W

G

I

L

L

M

N
|
N

P

P

S

G

K

K

V

V

F

L

binding 2-ketobutyric acid: R318 (= R337), H369 (= H385), H376 (= H392), H414 (= H430)
binding flavin-adenine dinucleotide: P68 (= P84), G70 (= G86), T71 (≠ A87), G72 (= G88), T73 (= T89), G74 (≠ S90), G78 (= G94), V79 (≠ A95), L90 (= L106), P132 (= P148), G133 (≠ S149), A134 (≠ S150), G140 (= G158), M141 (≠ N159), A143 (≠ G161), T144 (≠ M162), A146 (≠ S164), S147 (≠ G165), E200 (= E214), G201 (= G215), I206 (≠ V220), W322 (≠ F341), E413 (= E429), H414 (= H430), N450 (= N466)
binding manganese (ii) ion: H369 (= H385), H376 (= H392), E413 (= E429)

8jdeA Crystal structure of mldhd in complex with d-lactate (see paper)
28% identity, 87% coverage: 56:471/479 of query aligns to 40:455/455 of 8jdeA

query
sites
8jdeA

P

P

L

D

A

A

V

V

A

V

L

W

P

P

R

Q

T

N

T

V

A

D

E

Q

V

V

A

S

A

R

V

V

M

A

K

S

Y

L

C

C

H

Y

R

N

Y

Q

G

G

I

V

P

P

I

I

V

I

P
|
P

R

F

G
|
G

A
x
T

G
|
G

T
|
T

S
x
G

L

V

S

E

G

G

G
|
G

A
x
V

I

C

P

A

Q

V

E

Q

D

G

A

G

V

V

V

C

L

I

G

N

L
|
L

S

T

K

H

M

M

N

D

R

Q

I

I

L

T

D

E

I

L

D

N

Y

T

A

E

N

D

R

F

T

S

A

V

T

V

V

V

Q

E

A

P

G

G

V

V

T

T

N

R

L

K

N

A

V

L

S

N

E

T

S

H

V

L

S

R

A

D

D

S

G

G

F

L

F

W

Y

F

A

P

P

V

D

D

P
|
P

S
x
G

S
x
A

Q

D

L

-

A

-

C

A

T

S

I

L

G

C

G
|
G

N
x
M

I

A

G
x
A

M
x
T

N

G

S
x
A

G
x
S

G

G

A

T

H

N

C

A

L

V

K

R

Y

Y

G

G

V

T

T

M

T

R

N

D

N

N

L

V

L

I

G

N

V

L

K

E

L

V

V

V

L

L

T

P

D

D

G

G

T

R

I

L

D

H

L

T

G

A

G

G

K

R

A

G

L

R

-

H

-

Y

-

R

-

K

D

S

S

A

A

A

G

G

Y

Y

D

N

L

L

L

T

G

G

L

L

V

F

C

V

G

G

S

S

E
|
E

G
|
G

Q

T

L

L

G

G

I

I

V
x
I

T

T

E

S

A

T

T

T

V

L

R

R

L

L

I

H

A

P

K

A

P

P

E

E

G

A

A

T

R

V

P

A

V

A

L

T

F

C

G

A

F

F

D

P

T

S

S

V

E

Q

E

A

A

A

G

V

A

D

C

S

V

T

A

V

D

Q

V

I

I

L

A

Q

A

A

G

A

I

V

I

P

P

V

V

A

A

R

I

I

E

E

F

F

M

L

D

D

K

D

P

V

A

M

I

M

E

D

I

A

C

C

E

N

A

R

F

H

A

S

K

K

A

L

G

N

Y

C

P

P

L

-

D

-

V

V

G

A

A

P

L

T

L

L

I

F

V

L

E

E

V

F

E

H

G

G

S

S

E

Q

A

Q

E

T

M

L

D

A

D

E

M

Q

L

L

A

Q

S

R

I

T

V

E

E

A

I

I

A

T

R

Q

S

D

H

N

S

G

V

G

K

S

T

-

V

-

R

-

E

H

C

F

Q

S

S

W

A

A

T

A

E

E

A

K

A

N

L

E

I

L

W

W

K

A

G

A

R
|
R

K

H

S

N

A

A

F
x
W

G

Y

A

A

T

A

G

L

R

A

I

L

A

S

-

P

-

G

-

S

D

K

Y

A

I

Y

C

S

M

T

D

D

G

V

T

C

V

V

P

P

L

I

S

S

Q

R

L

L

S

P

Y

E

V

I

L

L

K

V

K

E

T

T

A

K

E

E

I

E

V

I

H

K

G

A

Y

S

G

K

L

L

R

T

V

G

A

A

N

I

V

V

F

G

H
|
H

A

V

G

G

D

D

G

G

N

N

M

F

H
|
H

P

C

L

I

I

L

L

L

F

V

N

D

A

P

N

D

D

D

P

A

E

E

D

E

A

Q

A

R

K

R

A

V

E

K

A

A

A

F

G

A

N

E

D

N

I

L

L

G

R

R

L

R

C

A

V

L

D

A

A

L

G

G

C

T

L

C

T

T

G

G

E
|
E

H
|
H

G

G

V

I

G

G

I

L

E

G

K

K

R

R

D

Q

L

L

M

L

R

Q

H

E

Q

E

Y

V

A

G

K

P

V

V

D

G

L

V

D

E

Q

T

M

M

M

R

A

Q

V

L

R

K

A

N

A

T

F

L

D

D

P

P

G

R

W

G

I

L

L

M

N
|
N

P

P

S

G

K

K

V

V

F

L

binding flavin-adenine dinucleotide: P68 (= P84), G70 (= G86), T71 (≠ A87), G72 (= G88), T73 (= T89), G74 (≠ S90), G78 (= G94), V79 (≠ A95), L90 (= L106), P132 (= P148), G133 (≠ S149), A134 (≠ S150), G140 (= G158), M141 (≠ N159), A143 (≠ G161), T144 (≠ M162), A146 (≠ S164), S147 (≠ G165), E200 (= E214), G201 (= G215), I206 (≠ V220), W322 (≠ F341), E413 (= E429), H414 (= H430), N450 (= N466)
binding lactic acid: R318 (= R337), H369 (= H385), H376 (= H392), H414 (= H430)
binding manganese (ii) ion: H369 (= H385), H376 (= H392), E413 (= E429)

8jdvA Crystal structure of mldhd in complex with 2-ketohexanoic acid (see paper)
28% identity, 87% coverage: 56:471/479 of query aligns to 40:454/454 of 8jdvA

query
sites
8jdvA

P

P

L

D

A

A

V

V

A

V

L

W

P

P

R

Q

T

N

T

V

A

D

E

Q

V

V

A

S

A

R

V

V

M

A

K

S

Y

L

C

C

H

Y

R

N

Y

Q

G

G

I

V

P

P

I

I

V

I

P
|
P

R

F

G
|
G

A
x
T

G
|
G

T
|
T

S
x
G

L
x
V

S

E

G

G

G
|
G

A
x
V

I

C

P

A

Q

V

E

Q

D

G

A

G

V

V

V

C

L

I

G

N

L
|
L

S

T

K

H

M

M

N

D

R

Q

I

I

L

T

D

E

I

L

D

N

Y

T

A

E

N

D

R

F

T

S

A

V

T

V

V

V

Q

E

A

P

G

G

V

V

T

T

N

R

L

K

N

A

V

L

S

N

E

T

S

H

V

L

S

R

A

D

D

S

G

G

F

L

F

W

Y

F

A

P

P

V

D

D

P
|
P

S
x
G

S
x
A

Q

D

L

-

A

-

C

A

T

S

I

L

G

C

G
|
G

N
x
M

I

A

G
x
A

M
x
T

N

G

S
x
A

G
x
S

G

G

A

T

H

N

C

A

L

V

K

R

Y

Y

G

G

V

T

T

M

T

R

N

D

N

N

L

V

L

I

G

N

V

L

K

E

L

V

V

V

L

L

T

P

D

D

G

G

T

R

I

L

D

H

L

T

G

A

G

G

K

R

A

G

L

R

-

H

-

Y

-

R

-

K

D

S

S

A

A

A

G

G

Y

Y

D

N

L

L

L

T

G

G

L

L

V

F

C

V

G

G

S

S

E
|
E

G
|
G

Q

T

L

L

G

G

I

I

V
x
I

T

T

E

S

A

T

T

T

V

L

R

R

L

L

I

H

A

P

K

A

P

P

E

E

G

A

A

T

R

V

P

A

V

A

L

T

F

C

G

A

F

F

D

P

T

S

S

V

E

Q

E

A

A

A

G

V

A

D

C

S

V

T

A

V

D

Q

V

I

I

L

A

Q

A

A

G

A

I

V

I

P

P

V

V

A

A

R

I

I

E

E

F

F

M

L

D

D

K

D

P

V

A

M

I

M

E

D

I

A

C

C

E

N

A

R

F

H

A

S

K

K

A

L

G

N

Y

C

P

P

L

-

D

-

V

V

G

A

A

P

L

T

L

L

I

F

V

L

E

E

V

F

E

H

G

G

S

S

E

Q

A

Q

E

T

M

L

D

A

D

E

M

Q

L

L

A

Q

S

R

I

T

V

E

E

A

I

I

A

T

R

Q

S

D

H

N

S

G

V

G

K

S

T

H

V

F

R

-

E

-

C

-

Q

-

S

S

A

A

T

E

E

K

A

R

A

N

L

E

I

L

W

W

K

A

G

A

R
|
R

K

H

S

N

A

A

F
x
W

G
x
Y

A

A

T

A

G

L

R

A

I

L

A

S

-

P

-

G

-

S

D

K

Y

A

I

Y

C

S

M

T

D

D

G

V

T

C

V

V

P

P

L

I

S

S

Q

R

L

L

S

P

Y

E

V

I

L

L

K

V

K

E

T

T

A

K

E

E

I

E

V

I

H

K

G

A

Y

S

G

K

L

L

R

T

V

G

A

A

N

I

V

V

F

G

H
|
H

A

V

G

G

D

D

G

G

N

N

M

F

H
|
H

P

C

L

I

I

L

L

L

F

V

N

D

A

P

N

D

D

D

P

A

E

E

D

E

A

Q

A

R

K

R

A

V

E

K

A

A

A

F

G

A

N

E

D

N

I

L

L

G

R

R

L

R

C

A

V

L

D

A

A

L

G

G

C

T

L

C

T

T

G

G

E
|
E

H
|
H

G

G

V

I

G

G

I

L

E

G

K

K

R

R

D

Q

L

L

M

L

R

Q

H

E

Q

E

Y

V

A

G

K

P

V

V

D

G

L

V

D

E

Q

T

M

M

M

R

A

Q

V

L

R

K

A

N

A

T

F

L

D

D

P

P

G

R

W

G

I

L

L

M

N
|
N

P

P

S

G

K

K

V

V

F

L

binding 2-Ketohexanoic acid: V75 (≠ L91), R317 (= R337), W321 (≠ F341), H368 (= H385), H375 (= H392), H413 (= H430)
binding flavin-adenine dinucleotide: P68 (= P84), G70 (= G86), T71 (≠ A87), G72 (= G88), T73 (= T89), G74 (≠ S90), G78 (= G94), V79 (≠ A95), L90 (= L106), P132 (= P148), G133 (≠ S149), A134 (≠ S150), G140 (= G158), M141 (≠ N159), A143 (≠ G161), T144 (≠ M162), A146 (≠ S164), S147 (≠ G165), E200 (= E214), G201 (= G215), I206 (≠ V220), W321 (≠ F341), Y322 (≠ G342), E412 (= E429), H413 (= H430), N449 (= N466)
binding manganese (ii) ion: H368 (= H385), H375 (= H392), E412 (= E429)

8jduA Crystal structure of mldhd in complex with 2-ketovaleric acid (see paper)
28% identity, 87% coverage: 56:471/479 of query aligns to 40:455/455 of 8jduA

query
sites
8jduA

P

P

L

D

A

A

V

V

A

V

L

W

P

P

R

Q

T

N

T

V

A

D

E

Q

V

V

A

S

A

R

V

V

M

A

K

S

Y

L

C

C

H

Y

R

N

Y

Q

G

G

I

V

P

P

I

I

V

I

P
|
P

R

F

G
|
G

A
x
T

G
|
G

T
|
T

S
x
G

L

V

S

E

G

G

G
|
G

A
x
V

I

C

P

A

Q

V

E

Q

D

G

A

G

V

V

V

C

L

I

G

N

L
|
L

S

T

K

H

M

M

N

D

R

Q

I

I

L

T

D

E

I

L

D

N

Y

T

A

E

N

D

R

F

T

S

A

V

T

V

V

V

Q

E

A

P

G

G

V

V

T

T

N

R

L

K

N

A

V

L

S

N

E

T

S

H

V

L

S

R

A

D

D

S

G

G

F

L

F

W

Y

F

A

P

P

V

D

D

P
|
P

S
x
G

S
x
A

Q

D

L

-

A

-

C

A

T

S

I

L

G

C

G
|
G

N
x
M

I

A

G
x
A

M
x
T

N

G

S
x
A

G
x
S

G

G

A

T

H

N

C

A

L

V

K

R

Y

Y

G

G

V

T

T

M

T

R

N

D

N

N

L

V

L

I

G

N

V

L

K

E

L

V

V

V

L

L

T

P

D

D

G

G

T

R

I

L

D

H

L

T

G

A

G

G

K

R

A

G

L

R

-

H

-

Y

-

R

-

K

D

S

S

A

A

A

G

G

Y

Y

D

N

L

L

L

T

G

G

L

L

V

F

C

V

G

G

S

S

E
|
E

G
|
G

Q

T

L

L

G

G

I

I

V
x
I

T

T

E

S

A

T

T

T

V

L

R

R

L

L

I

H

A

P

K

A

P

P

E

E

G

A

A

T

R

V

P

A

V

A

L

T

F

C

G

A

F

F

D

P

T

S

S

V

E

Q

E

A

A

A

G

V

A

D

C

S

V

T

A

V

D

Q

V

I

I

L

A

Q

A

A

G

A

I

V

I

P

P

V

V

A

A

R

I

I

E

E

F

F

M

L

D

D

K

D

P

V

A

M

I

M

E

D

I

A

C

C

E

N

A

R

F

H

A

S

K

K

A

L

G

N

Y

C

P

P

L

-

D

-

V

V

G

A

A

P

L

T

L

L

I

F

V

L

E

E

V

F

E

H

G

G

S

S

E

Q

A

Q

E

T

M

L

D

A

D

E

M

Q

L

L

A

Q

S

R

I

T

V

E

E

A

I

I

A

T

R

Q

S

N

H

G

S

G

V

S

K

H

T

F

V

S

R

W

E

A

C

A

Q

E

S

K

A

R

T

N

E

E

A

-

L

-

I

L

W

W

K

A

G

A

R
|
R

K

H

S

N

A

A

F
x
W

G

Y

A

A

T

A

G

L

R

A

I

L

A

S

-

P

-

G

-

S

D

K

Y

A

I

Y

C

S

M

T

D

D

G

V

T

C

V

V

P

P

L

I

S

S

Q

R

L

L

S

P

Y

E

V

I

L

L

K

V

K

E

T

T

A

K

E

E

I

E

V

I

H

K

G

A

Y

S

G

K

L

L

R

T

V

G

A

A

N

I

V

V

F

G

H
|
H

A

V

G

G

D

D

G

G

N

N

M

F

H
|
H

P

C

L

I

I

L

L

L

F

V

N

D

A

P

N

D

D

D

P

A

E

E

D

E

A

Q

A

R

K

R

A

V

E

K

A

A

A

F

G

A

N

E

D

N

I

L

L

G

R

R

L

R

C

A

V

L

D

A

A

L

G

G

C

T

L

C

T

T

G

G

E
|
E

H
|
H

G

G

V

I

G

G

I

L

E

G

K

K

R

R

D

Q

L

L

M

L

R

Q

H

E

Q

E

Y

V

A

G

K

P

V

V

D

G

L

V

D

E

Q

T

M

M

M

R

A

Q

V

L

R

K

A

N

A

T

F

L

D

D

P

P

G

R

W

G

I

L

L

M

N
|
N

P

P

S

G

K

K

V

V

F

L

binding 2-oxopentanoic acid: R318 (= R337), W322 (≠ F341), H369 (= H385), H376 (= H392), H414 (= H430)
binding flavin-adenine dinucleotide: P68 (= P84), G70 (= G86), T71 (≠ A87), G72 (= G88), T73 (= T89), G74 (≠ S90), G78 (= G94), V79 (≠ A95), L90 (= L106), P132 (= P148), G133 (≠ S149), A134 (≠ S150), G140 (= G158), M141 (≠ N159), A143 (≠ G161), T144 (≠ M162), A146 (≠ S164), S147 (≠ G165), E200 (= E214), G201 (= G215), I206 (≠ V220), W322 (≠ F341), E413 (= E429), N450 (= N466)
binding manganese (ii) ion: H369 (= H385), H376 (= H392), E413 (= E429)

8jdsA Crystal structure of mldhd in complex with pyruvate (see paper)
28% identity, 87% coverage: 56:471/479 of query aligns to 40:456/456 of 8jdsA

query
sites
8jdsA

P

P

L

D

A

A

V

V

A

V

L

W

P

P

R

Q

T

N

T

V

A

D

E

Q

V

V

A

S

A

R

V

V

M

A

K

S

Y

L

C

C

H

Y

R

N

Y

Q

G

G

I

V

P

P

I

I

V

I

P
|
P

R

F

G
|
G

A
x
T

G
|
G

T
|
T

S
x
G

L

V

S

E

G

G

G
|
G

A
x
V

I

C

P

A

Q

V

E

Q

D

G

A

G

V

V

V

C

L

I

G

N

L
|
L

S

T

K

H

M

M

N

D

R

Q

I

I

L

T

D

E

I

L

D

N

Y

T

A

E

N

D

R

F

T

S

A

V

T

V

V

V

Q

E

A

P

G

G

V

V

T

T

N

R

L

K

N

A

V

L

S

N

E

T

S

H

V

L

S

R

A

D

D

S

G

G

F

L

F

W

Y

F

A

P

P

V

D

D

P
|
P

S
x
G

S
x
A

Q

D

L

-

A

-

C

A

T

S

I

L

G

C

G
|
G

N
x
M

I

A

G
x
A

M
x
T

N

G

S
x
A

G
x
S

G

G

A

T

H

N

C

A

L

V

K

R

Y

Y

G

G

V

T

T

M

T

R

N

D

N

N

L

V

L

I

G

N

V

L

K

E

L

V

V

V

L

L

T

P

D

D

G

G

T

R

I

L

D

H

L

T

G

A

G

G

K

R

A

G

L

R

-

H

-

Y

-

R

-

K

D

S

S

A

A

A

G

G

Y

Y

D

N

L

L

L

T

G

G

L

L

V

F

C

V

G

G

S

S

E
|
E

G
|
G

Q

T

L

L

G

G

I

I

V
x
I

T

T

E

S

A

T

T

T

V

L

R

R

L

L

I

H

A

P

K

A

P

P

E

E

G

A

A

T

R

V

P

A

V

A

L

T

F

C

G

A

F

F

D

P

T

S

S

V

E

Q

E

A

A

A

G

V

A

D

C

S

V

T

A

V

D

Q

V

I

I

L

A

Q

A

A

G

A

I

V

I

P

P

V

V

A

A

R

I

I

E

E

F

F

M

L

D

D

K

D

P

V

A

M

I

M

E

D

I

A

C

C

E

N

A

R

F

H

A

S

K

K

A

L

G

N

Y

C

P

P

L

-

D

-

V

V

G

A

A

P

L

T

L

L

I

F

V

L

E

E

V

F

E

H

G

G

S

S

E

Q

A

Q

E

T

M

L

D

A

D

E

M

Q

L

L

A

Q

S

R

I

T

V

E

E

A

I

I

A

T

R

Q

S

D

H

N

S

G

V

G

K

S

T

H

V

F

R

-

E

-

C

S

Q

W

S

A

A

A

T

E

E

K

A

R

A

N

L

E

I

L

W

W

K

A

G

A

R
|
R

K

H

S

N

A

A

F
x
W

G

Y

A

A

T

A

G

L

R

A

I

L

A

S

-

P

-

G

-

S

D

K

Y

A

I

Y

C

S

M

T

D

D

G

V

T

C

V

V

P

P

L

I

S

S

Q

R

L

L

S

P

Y

E

V

I

L

L

K

V

K

E

T

T

A

K

E

E

I

E

V

I

H

K

G

A

Y

S

G

K

L

L

R

T

V

G

A

A

N

I

V

V

F

G

H
|
H

A

V

G

G

D

D

G

G

N

N

M

F

H
|
H

P

C

L

I

I

L

L

L

F

V

N

D

A

P

N

D

D

D

P

A

E

E

D

E

A

Q

A

R

K

R

A

V

E

K

A

A

A

F

G

A

N

E

D

N

I

L

L

G

R

R

L

R

C

A

V

L

D

A

A

L

G

G

C

T

L

C

T

T

G

G

E
|
E

H
|
H

G

G

V

I

G

G

I

L

E

G

K

K

R

R

D

Q

L

L

M

L

R

Q

H

E

Q

E

Y

V

A

G

K

P

V

V

D

G

L

V

D

E

Q

T

M

M

M

R

A

Q

V

L

R

K

A

N

A

T

F

L

D

D

P

P

G

R

W

G

I

L

L

M

N
|
N

P

P

S

G

K

K

V

V

F

L

binding flavin-adenine dinucleotide: P68 (= P84), G70 (= G86), T71 (≠ A87), G72 (= G88), T73 (= T89), G74 (≠ S90), G78 (= G94), V79 (≠ A95), L90 (= L106), P132 (= P148), G133 (≠ S149), A134 (≠ S150), G140 (= G158), M141 (≠ N159), A143 (≠ G161), T144 (≠ M162), A146 (≠ S164), S147 (≠ G165), E200 (= E214), G201 (= G215), I206 (≠ V220), W323 (≠ F341), E414 (= E429), H415 (= H430), N451 (= N466)
binding manganese (ii) ion: H370 (= H385), H377 (= H392), E414 (= E429)
binding pyruvic acid: R319 (= R337), H370 (= H385), H377 (= H392), H415 (= H430)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 32

8jdpA Crystal structure of h405a mldhd in complex with d-2-hydroxyisovaleric acid (see paper)
28% identity, 87% coverage: 56:471/479 of query aligns to 40:455/455 of 8jdpA

query
sites
8jdpA

P

P

L

D

A

A

V

V

A

V

L

W

P

P

R

Q

T

N

T

V

A

D

E

Q

V

V

A

S

A

R

V

V

M

A

K

S

Y

L

C

C

H

Y

R

N

Y

Q

G

G

I

V

P

P

I

I

V

I

P
|
P

R

F

G
|
G

A
x
T

G
|
G

T
|
T

S
x
G

L

V

S

E

G

G

G
|
G

A
x
V

I

C

P

A

Q

V

E

Q

D

G

A

G

V

V

V

C

L

I

G

N

L
|
L

S

T

K

H

M

M

N

D

R

Q

I

I

L

T

D

E

I

L

D

N

Y

T

A

E

N

D

R

F

T

S

A

V

T

V

V

V

Q

E

A

P

G

G

V

V

T

T

N

R

L

K

N

A

V

L

S

N

E

T

S

H

V

L

S

R

A

D

D

S

G

G

F

L

F

W

Y

F

A

P

P

V

D

D

P
|
P

S
x
G

S
x
A

Q

D

L

-

A

-

C

A

T

S

I

L

G

C

G
|
G

N
x
M

I

A

G
x
A

M
x
T

N

G

S
x
A

G
x
S

G

G

A

T

H

N

C

A

L

V

K

R

Y

Y

G

G

V

T

T

M

T

R

N

D

N

N

L

V

L

I

G

N

V

L

K

E

L

V

V

V

L

L

T

P

D

D

G

G

T

R

I

L

D

H

L

T

G

A

G

G

K

R

A

G

L

R

-

H

-

Y

-

R

-

K

D

S

S

A

A

A

G

G

Y

Y

D

N

L

L

L

T

G

G

L

L

V

F

C

V

G

G

S

S

E
|
E

G
|
G

Q

T

L

L

G

G

I

I

V
x
I

T

T

E

S

A

T

T

T

V

L

R

R

L

L

I

H

A

P

K

A

P

P

E

E

G

A

A

T

R

V

P

A

V

A

L

T

F

C

G

A

F

F

D

P

T

S

S

V

E

Q

E

A

A

A

G

V

A

D

C

S

V

T

A

V

D

Q

V

I

I

L

A

Q

A

A

G

A

I

V

I

P

P

V

V

A

A

R

I

I

E

E

F

F

M

L

D

D

K

D

P

V

A

M

I

M

E

D

I

A

C

C

E

N

A

R

F

H

A

S

K

K

A

L

G

N

Y

C

P

P

L

-

D

-

V

V

G

A

A

P

L

T

L

L

I

F

V

L

E

E

V

F

E

H

G

G

S

S

E

Q

A

Q

E

T

M

L

D

A

D

E

M

Q

L

L

A

Q

S

R

I

T

V

E

E

A

I

I

A

T

R

Q

S

D

H

N

S

G

V

G

K

S

T

H

V

F

R

-

E

-

C

S

Q

W

S

A

A

A

T

E

E

K

A

R

A

N

L

E

I

L

W

W

K

A

G

A

R
|
R

K

H

S

N

A

A

F

W

G

Y

A

A

T

A

G

L

R

A

I

L

A

S

-

P

-

S

D

K

Y

A

I

Y

C

S

M

T

D

D

G

V

T

C

V

V

P

P

L

I

S

S

Q

R

L

L

S

P

Y

E

V

I

L

L

K

V

K

E

T

T

A

K

E

E

I

E

V

I

H

K

G

A

Y

S

G

K

L

L

R

T

V

G

A

A

N

I

V

V

F

G

H
|
H

A

V

G

G

D

D

G

G

N

N

M

F

H

A

P

C

L

I

I

L

L

L

F

V

N

D

A

P

N

D

D

D

P

A

E

E

D

E

A

Q

A

R

K

R

A

V

E

K

A

A

A

F

G

A

N

E

D

N

I

L

L

G

R

R

L

R

C

A

V

L

D

A

A

L

G

G

C

T

L

C

T

T

G

G

E
|
E

H
|
H

G

G

V

I

G

G

I

L

E

G

K

K

R

R

D

Q

L

L

M

L

R

Q

H

E

Q

E

Y

V

A

G

K

P

V

V

D

G

L

V

D

E

Q

T

M

M

M

R

A

Q

V

L

R

K

A

N

A

T

F

L

D

D

P

P

G

R

W

G

I

L

L

M

N
|
N

P

P

S

G

K

K

V

V

F

L

binding flavin-adenine dinucleotide: P68 (= P84), G70 (= G86), T71 (≠ A87), G72 (= G88), T73 (= T89), G74 (≠ S90), G78 (= G94), V79 (≠ A95), L90 (= L106), P132 (= P148), G133 (≠ S149), A134 (≠ S150), G140 (= G158), M141 (≠ N159), A143 (≠ G161), T144 (≠ M162), A146 (≠ S164), S147 (≠ G165), E200 (= E214), G201 (= G215), I206 (≠ V220), H369 (= H385), E413 (= E429), N450 (= N466)
binding deaminohydroxyvaline: R319 (= R337), H414 (= H430)

8jdrA Crystal structure of h405a mldhd in complex with d-2-hydroxy-3-methyl- valeric acid (see paper)
28% identity, 87% coverage: 56:471/479 of query aligns to 40:456/456 of 8jdrA

query
sites
8jdrA

P

P

L

D

A

A

V

V

A

V

L

W

P

P

R

Q

T

N

T

V

A

D

E

Q

V

V

A

S

A

R

V

V

M

A

K

S

Y

L

C

C

H

Y

R

N

Y

Q

G

G

I

V

P

P

I

I

V

I

P
|
P

R

F

G
|
G

A
x
T

G
|
G

T
|
T

S
x
G

L

V

S

E

G

G

G
|
G

A
x
V

I

C

P

A

Q

V

E

Q

D

G

A

G

V

V

V

C

L

I

G

N

L
|
L

S

T

K

H

M

M

N

D

R

Q

I

I

L

T

D

E

I

L

D

N

Y

T

A

E

N

D

R

F

T

S

A

V

T

V

V

V

Q

E

A

P

G

G

V

V

T

T

N

R

L

K

N

A

V

L

S

N

E

T

S

H

V

L

S

R

A

D

D

S

G

G

F

L

F

W

Y

F

A

P

P

V

D

D

P
|
P

S
x
G

S
x
A

Q

D

L

-

A

-

C

A

T

S

I

L

G

C

G
|
G

N
x
M

I

A

G
x
A

M
x
T

N

G

S
x
A

G
x
S

G

G

A

T

H

N

C

A

L

V

K

R

Y

Y

G

G

V

T

T

M

T

R

N

D

N

N

L

V

L

I

G

N

V

L

K

E

L

V

V

V

L

L

T

P

D

D

G

G

T

R

I

L

D

H

L

T

G

A

G

G

K

R

A

G

L

R

-

H

-

Y

-

R

-

K

D

S

S

A

A

A

G

G

Y

Y

D

N

L

L

L

T

G

G

L

L

V

F

C

V

G

G

S

S

E
|
E

G
|
G

Q

T

L

L

G

G

I

I

V
x
I

T

T

E

S

A

T

T

T

V

L

R

R

L

L

I

H

A

P

K

A

P

P

E

E

G

A

A

T

R

V

P

A

V

A

L

T

F

C

G

A

F

F

D

P

T

S

S

V

E

Q

E

A

A

A

G

V

A

D

C

S

V

T

A

V

D

Q

V

I

I

L

A

Q

A

A

G

A

I

V

I

P

P

V

V

A

A

R

I

I

E

E

F

F

M

L

D

D

K

D

P

V

A

M

I

M

E

D

I

A

C

C

E

N

A

R

F

H

A

S

K

K

A

L

G

N

Y

C

P

P

L

-

D

-

V

V

G

A

A

P

L

T

L

L

I

F

V

L

E

E

V

F

E

H

G

G

S

S

E

Q

A

Q

E

T

M

L

D

A

D

E

M

Q

L

L

A

Q

S

R

I

T

V

E

E

A

I

I

A

T

R

Q

S

D

H

N

S

G

V

G

K

S

T

H

V

F

R

-

E

-

C

S

Q

W

S

A

A

A

T

E

E

K

A

R

A

N

L

E

I

L

W

W

K

A

G

A

R
|
R

K

H

S

N

A

A

F
x
W

G
x
Y

A

A

T

A

G

L

R

A

I

L

A

S

-

P

-

G

-

S

D

K

Y

A

I

Y

C

S

M

T

D

D

G

V

T

C