SitesBLAST

P39976 D-2-hydroxyglutarate--pyruvate transhydrogenase DLD3; D-2HG--pyruvate transhydrogenase DLD3; (R)-2-hydroxyglutarate--pyruvate transhydrogenase; D-lactate dehydrogenase [cytochrome] 3; D-lactate ferricytochrome C oxidoreductase; D-LCR; EC 1.1.99.40; EC 1.1.2.4 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
39% identity, 94% coverage: 27:474/476 of query aligns to 50:496/496 of P39976

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Sites not aligning to the query:

17 modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)

6lpxA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with 2-oxoglutarate (2-og) (see paper)
37% identity, 95% coverage: 24:474/476 of query aligns to 26:464/466 of 6lpxA

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binding 2-oxoglutaric acid: R333 (= R336), T337 (≠ S340), K348 (= K351), Y379 (≠ F384), H381 (= H386), H388 (= H393), H423 (= H433)
binding flavin-adenine dinucleotide: W39 (≠ E36), P75 (= P73), Q76 (= Q74), G77 (≠ T75), G78 (= G76), N79 (= N77), T80 (= T78), G81 (= G79), M82 (≠ L80), G85 (= G83), S86 (≠ Q84), L139 (= L139), G140 (= G140), A141 (≠ S141), C145 (= C145), G149 (= G149), N150 (= N150), A152 (≠ S152), T153 (= T153), G157 (= G157), G207 (= G207), I212 (= I212), E422 (= E432), N459 (= N469)
binding zinc ion: H381 (= H386), H388 (= H393), E422 (= E432)

6lpuA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with l-2-hydroxyglutarate (l-2-hg) (see paper)
37% identity, 95% coverage: 24:474/476 of query aligns to 26:464/466 of 6lpuA

query
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6lpuA

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Q

D

F

I

M

V

A

T

E

D

A

L

E

R

K

A

A

R

V

L

V

-

A

-

M

G

P

P

G

H

A

A

R

K

-

H

V

V

C

V

A

G

F
x
Y

G

G

H
|
H

M

L

G

G

D

D

G

G

N

N

I

L

H
|
H

Y

L

N

N

I

V

S

T

Q

A

P

-

V

-

G

-

A

-

D

-

K

E

A

A

E

F

F

S

I

P

G

S

R

L

W

L

R

A

E

A

M

L

N

E

K

P

I

H

V

V

H

Y

G

E

L

W

V

T

L

A

Q

G

H

Q

G

Q

G

G

S

S

I

V

S

S

A

A

E
|
E

H
|
H

G

G

I

V

G

G

Q

F

L

R

K

K

R

R

D

D

E

V

L

L

A

G

S

Y

I

S

R

K

S

P

D

P

I

G

E

A

I

L

D

Q

L

L

M

M

R

Q

I

L

K

K

T

A

A

L

F

L

D

D

P

P

A

K

G

G

I

I

M

L

N
|
N

P

P

G

Y

K

K

V

T

L

L

binding flavin-adenine dinucleotide: W39 (≠ E36), P75 (= P73), G77 (≠ T75), G78 (= G76), N79 (= N77), T80 (= T78), G81 (= G79), G85 (= G83), S86 (≠ Q84), L139 (= L139), G140 (= G140), A141 (≠ S141), C145 (= C145), H146 (≠ R146), G148 (≠ A148), G149 (= G149), N150 (= N150), A152 (≠ S152), T153 (= T153), A155 (= A155), E206 (= E206), G207 (= G207), I211 (≠ V211), I212 (= I212), E422 (= E432), N459 (= N469)
binding (2s)-2-hydroxypentanedioic acid: R333 (= R336), T337 (≠ S340), K348 (= K351), Y379 (≠ F384), H381 (= H386), H388 (= H393), H423 (= H433)
binding zinc ion: H381 (= H386), H388 (= H393), E422 (= E432)

6lpqA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with d-malate (d-mal) (see paper)
37% identity, 95% coverage: 24:474/476 of query aligns to 26:464/466 of 6lpqA

query
sites
6lpqA

V

V

R

T

D

D

P

P

A

E

E

A

I

L

-

Q

A

A

P

P

H

N

L

V

V

D

E
x
W

N

L

R

R

G

T

L

L

Y

-

H

R

G

G

A

C

S

S

P

K

M

V

L

L

K

R

P

P

G

R

S

T

V

S

E

E

V

V

S

S

S

H

I

I

L

L

K

R

L

H

A

C

S

H

E

E

T

R

G

N

A

L

A

A

I

V

V

N

P
|
P

Q

Q

T
x
G

G
|
G

N
|
N

T
|
T

G
|
G

L
x
M

V

V

G

G

G
|
G

Q
x
S

T

V

P

P

R

V

E

F

G

-

S

D

D

E

I

I

L

L

S

S

L

T

E

A

R

R

M

M

N

N

R

R

V

V

R

L

D

S

V

F

D

H

P

S

V

V

G

S

N

G

T

I

I

L

V

V

V

C

D

Q

G

A

G

G

C

C

I

V

L

L

A

E

D

E

V

L

H

S

K

R

A

Y

A

V

A

E

D

E

H

R

G

D

R

F

M

I

F

M

P

P

L

L

S

D

L
|
L

G
|
G

S
x
A

E

K

G

G

S

S

C
|
C

R
x
H

I

I

A

G

G
|
G

N
|
N

L

V

S
x
A

T
|
T

N

N

A
|
A

G

G

G
|
G

T

L

A

R

V

F

L

L

A

R

Y

Y

G

G

N

S

M

L

R

H

Q

G

L

T

C

V

L

L

G

G

L

L

E

E

V

V

L

L

P

A

T

D

G

G

E

T

I

V

W

L

N

D

G

C

L

L

R

T

R

S

L

L

K

R

K

K

D

D

N

N

T

T

G

G

Y

Y

D

D

L

L

R

K

D

Q

L

L

F

F

V

I

G

G

A

S

E
|
E

G
|
G

T

T

L

L

G

G

V
x
I

I
|
I

T

T

G

T

A

V

V

S

L

I

K

L

L

C

F

P

P

P

Q

K

P

P

L

R

G

A

H

V

Q

N

V

V

A

A

F

F

A

L

G

G

L

C

Q

P

S

G

V

F

D

A

D

E

A

V

L

L

T

Q

L

T

F

F

K

S

N

T

A

C

S

K

S

G

L

M

C

L

G

G

T

E

A

I

L

L

T

S

G

A

F

F

E

E

L

F

M

M

P

D

R

A

I

V

G

C

V

M

E

Q

F

L

T

V

A

G

R

R

H

H

I

L

P

-

G

H

V

L

R

A

D

S

P

P

L

V

E

Q

T

E

A

S

H

-

P

P

W

F

Y

Y

V

V

L

L

I

I

D

E

I

T

S

S

T

G

S

S

D

N

S

A

A

G

E

H

T

D

A

A

E

E

R

K

M

-

M

L

A

G

A

H

L

F

L

L

E

E

Q

H

G

A

Y

L

E

G

A

S

G

G

L

L

I

V

Q

T

D

D

A

G

T

T

I

M

A

A

S

T

S

D

E

Q

A

R

Q

K

R

V

Q

K

A

M

I

L

W

W

H

A

M

L

R
|
R

E

E

S

R

M

I

S
x
T

D

E

A

A

Q

L

K

S

P

R

E

D

G

G

G

Y

S

V

I

Y

K
|
K

H

Y

D

D

V

L

S

S

V

L

P

P

V

V

A

E

Q

R

I

L

P

Y

Q

D

F

I

M

V

A

T

E

D

A

L

E

R

K

A

A

R

V

L

V

-

A

-

M

G

P

P

G

H

A

A

R

K

-

H

V

V

C

V

A

G

F
x
Y

G

G

H
|
H

M

L

G

G

D

D

G

G

N

N

I

L

H
|
H

Y

L

N

N

I

V

S

T

Q

A

P

-

V

-

G

-

A

-

D

-

K

E

A

A

E

F

F

S

I

P

G

S

R

L

W

L

R

A

E

A

M

L

N

E

K

P

I

H

V

V

H

Y

G

E

L

W

V

T

L

A

Q

G

H

Q

G

Q

G

G

S

S

I

V

S

S

A

A

E
|
E

H
|
H

G

G

I

V

G

G

Q

F

L

R

K

K

R

R

D

D

E

V

L

L

A

G

S

Y

I

S

R

K

S

P

D

P

I

G

E

A

I

L

D

Q

L

L

M

M

R

Q

I

L

K

K

T

A

A

L

F

L

D

D

P

P

A

K

G

G

I

I

M

L

N
|
N

P

P

G

Y

K

K

V

T

L

L

binding flavin-adenine dinucleotide: W39 (≠ E36), P75 (= P73), G77 (≠ T75), G78 (= G76), N79 (= N77), T80 (= T78), G81 (= G79), G85 (= G83), S86 (≠ Q84), L139 (= L139), G140 (= G140), A141 (≠ S141), C145 (= C145), H146 (≠ R146), G149 (= G149), N150 (= N150), A152 (≠ S152), T153 (= T153), A155 (= A155), G157 (= G157), E206 (= E206), G207 (= G207), I211 (≠ V211), I212 (= I212), E422 (= E432), N459 (= N469)
binding d-malate: M82 (≠ L80), R333 (= R336), T337 (≠ S340), K348 (= K351), Y379 (≠ F384), H381 (= H386), H388 (= H393), E422 (= E432), H423 (= H433)
binding zinc ion: H381 (= H386), H388 (= H393), E422 (= E432)

6lppA Crystal structure of human d-2-hydroxyglutarate dehydrogenase in complex with d-2-hydroxyglutarate (d-2-hg) (see paper)
37% identity, 95% coverage: 24:474/476 of query aligns to 26:464/466 of 6lppA

query
sites
6lppA

V

V

R

T

D

D

P

P

A

E

E

A

I

L

-

Q

A

A

P

P

H

N

L

V

V

D

E
x
W

N

L

R

R

G

T

L

L

Y

-

H

R

G

G

A

C

S

S

P

K

M

V

L

L

K

R

P

P

G

R

S

T

V

S

E

E

V

V

S

S

S

H

I

I

L

L

K

R

L

H

A

C

S

H

E

E

T

R

G

N

A

L

A

A

I

V

V

N

P
|
P

Q

Q

T
x
G

G
|
G

N
|
N

T
|
T

G
|
G

L
x
M

V

V

G

G

G
|
G

Q
x
S

T

V

P

P

R

V

E

F

G

-

S

D

D

E

I

I

L

L

S

S

L

T

E

A

R

R

M

M

N

N

R

R

V

V

R

L

D

S

V

F

D

H

P

S

V

V

G

S

N

G

T

I

I

L

V

V

V

C

D

Q

G

A

G

G

C

C

I

V

L

L

A

E

D

E

V

L

H

S

K

R

A

Y

A

V

A

E

D

E

H

R

G

D

R

F

M

I

F

M

P

P

L

L

S

D

L
|
L

G
|
G

S
x
A

E

K

G

G

S

S

C
|
C

R

H

I

I

A

G

G
|
G

N
|
N

L

V

S
x
A

T
|
T

N

N

A
|
A

G

G

G
|
G

T

L

A

R

V

F

L

L

A

R

Y

Y

G

G

N

S

M

L

R

H

Q

G

L

T

C

V

L

L

G

G

L

L

E

E

V

V

L

L

P

A

T

D

G

G

E

T

I

V

W

L

N

D

G

C

L

L

R

T

R

S

L

L

K

R

K

K

D

D

N

N

T

T

G

G

Y

Y

D

D

L

L

R

K

D

Q

L

L

F

F

V

I

G

G

A

S

E

E

G
|
G

T

T

L

L

G

G

V

I

I
|
I

T

T

G

T

A

V

V

S

L

I

K

L

L

C

F

P

P

P

Q

K

P

P

L

R

G

A

H

V

Q

N

V

V

A

A

F

F

A

L

G

G

L

C

Q

P

S

G

V

F

D

A

D

E

A

V

L

L

T

Q

L

T

F

F

K

S

N

T

A

C

S

K

S

G

L

M

C

L

G

G

T

E

A

I

L

L

T

S

G

A

F

F

E

E

L

F

M

M

P

D

R

A

I

V

G

C

V

M

E

Q

F

L

T

V

A

G

R

R

H

H

I

L

P

-

G

H

V

L

R

A

D

S

P

P

L

V

E

Q

T

E

A

S

H

-

P

P

W

F

Y

Y

V

V

L

L

I

I

D

E

I

T

S

S

T

G

S

S

D

N

S

A

A

G

E

H

T

D

A

A

E

E

R

K

M

-

M

L

A

G

A

H

L

F

L

L

E

E

Q

H

G

A

Y

L

E

G

A

S

G

G

L

L

I

V

Q

T

D

D

A

G

T

T

I

M

A

A

S

T

S

D

E

Q

A

R

Q

K

R

V

Q

K

A

M

I

L

W

W

H

A

M

L

R
|
R

E

E

S

R

M

I

S
x
T

D

E

A

A

Q

L

K

S

P

R

E

D

G

G

G

Y

S

V

I

Y

K
|
K

H

Y

D

D

V

L

S

S

V

L

P

P

V

V

A

E

Q

R

I

L

P

Y

Q

D

F

I

M

V

A

T

E

D

A

L

E

R

K

A

A

R

V

L

V

-

A

-

M

G

P

P

G

H

A

A

R

K

-

H

V

V

C

V

A

G

F
x
Y

G

G

H
|
H

M

L

G

G

D

D

G

G

N

N

I

L

H
|
H

Y

L

N
|
N

I

V

S

T

Q

A

P

-

V

-

G

-

A

-

D

-

K

E

A

A

E

F

F

S

I

P

G

S

R

L

W

L

R

A

E

A

M

L

N

E

K

P

I

H

V

V

H

Y

G

E

L

W

V

T

L

A

Q

G

H

Q

G

Q

G

G

S

S

I

V

S

S

A

A

E
|
E

H
|
H

G

G

I

V

G

G

Q

F

L

R

K

K

R

R

D

D

E

V

L

L

A

G

S

Y

I

S

R

K

S

P

D

P

I

G

E

A

I

L

D

Q

L

L

M

M

R

Q

I

L

K

K

T

A

A

L

F

L

D

D

P

P

A

K

G

G

I

I

M

L

N

N

P

P

G

Y

K

K

V

T

L

L

binding (2R)-2-hydroxypentanedioic acid: R333 (= R336), T337 (≠ S340), K348 (= K351), Y379 (≠ F384), H381 (= H386), H388 (= H393), N390 (= N395), H423 (= H433)
binding flavin-adenine dinucleotide: W39 (≠ E36), P75 (= P73), G77 (≠ T75), G78 (= G76), N79 (= N77), T80 (= T78), G81 (= G79), M82 (≠ L80), G85 (= G83), S86 (≠ Q84), L139 (= L139), G140 (= G140), A141 (≠ S141), C145 (= C145), G149 (= G149), N150 (= N150), A152 (≠ S152), T153 (= T153), A155 (= A155), G157 (= G157), G207 (= G207), I212 (= I212), E422 (= E432), H423 (= H433)
binding zinc ion: H381 (= H386), H388 (= H393), E422 (= E432)

6lpnB Crystal structure of human d-2-hydroxyglutarate dehydrogenase in apo form (see paper)
37% identity, 95% coverage: 24:474/476 of query aligns to 27:465/467 of 6lpnB

query
sites
6lpnB

V

V

R

T

D

D

P

P

A

E

E

A

I

L

-

Q

A

A

P

P

H

N

L

V

V

D

E
x
W

N

L

R

R

G

T

L

L

Y

-

H

R

G

G

A

C

S

S

P

K

M

V

L

L

K

R

P

P

G

R

S

T

V

S

E

E

V

V

S

S

S

H

I

I

L

L

K

R

L

H

A

C

S

H

E

E

T

R

G

N

A

L

A

A

I

V

V

N

P
|
P

Q

Q

T
x
G

G
|
G

N
|
N

T
|
T

G
|
G

L
x
M

V

V

G

G

G
|
G

Q
x
S

T

V

P

P

R

V

E

F

G

-

S

D

D

E

I

I

L

L

S

S

L

T

E

A

R

R

M

M

N

N

R

R

V

V

R

L

D

S

V

F

D

H

P

S

V

V

G

S

N

G

T

I

I

L

V

V

V

C

D

Q

G

A

G

G

C

C

I

V

L

L

A

E

D

E

V

L

H

S

K

R

A

Y

A

V

A

E

D

E

H

R

G

D

R

F

M

I

F

M

P

P

L

L

S

D

L
|
L

G

G

S
x
A

E

K

G

G

S

S

C
|
C

R
x
H

I

I

A

G

G
|
G

N
|
N

L

V

S
x
A

T
|
T

N

N

A

A

G

G

T

L

A

R

V

F

L

L

A

R

Y

Y

G

G

N

S

M

L

R

H

Q

G

L

T

C

V

L

L

G

G

L

L

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E

V

V

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-

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-

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Y

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-

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A

A

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-

A

-

M

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A

A

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-

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V

C

V

A

G

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G

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H

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G

G

D

D

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G

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N

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N

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A

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-

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-

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-

A

-

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-

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K

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T

A

A

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D

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G

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L

binding flavin-adenine dinucleotide: W40 (≠ E36), P76 (= P73), G78 (≠ T75), G79 (= G76), N80 (= N77), T81 (= T78), G82 (= G79), M83 (≠ L80), G86 (= G83), S87 (≠ Q84), L140 (= L139), A142 (≠ S141), C146 (= C145), H147 (≠ R146), G150 (= G149), N151 (= N150), A153 (≠ S152), T154 (= T153), G208 (= G207), I212 (≠ V211), I213 (= I212), E423 (= E432), N460 (= N469)

Q8N465 D-2-hydroxyglutarate dehydrogenase, mitochondrial; EC 1.1.99.39 from Homo sapiens (Human) (see 7 papers)
37% identity, 95% coverage: 24:474/476 of query aligns to 79:517/521 of Q8N465

query
sites
Q8N465

V

V

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D

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A
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x
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D

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x
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L

S109 (≠ V54) to W: in D2HGA1; uncertain significance; significant loss of catalytic activity; dbSNP:rs142050154
N127 (≠ V72) to K: in D2HGA1; uncertain significance; complete loss of catalytic activity; dbSNP:rs762857195
G131 (= G76) to V: in D2HGA1; uncertain significance; complete loss of catalytic activity
I147 (= I94) to S: in D2HGA1; uncertain significance; severe phenotype; almost complete loss of catalytic activity; dbSNP:rs121434361
M153 (= M100) to T: in D2HGA1; uncertain significance; significant loss of catalytic activity; to V: in D2HGA1; uncertain significance; significant loss of catalytic activity; dbSNP:rs1432270139
C172 (= C119) to Y: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs773735172
P189 (= P136) to L: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs587783517
A205 (≠ S152) to V: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs750889931
A231 (≠ P178) to V: in D2HGA1; uncertain significance; significant loss of catalytic activity
G233 (= G180) to S: in D2HGA1; uncertain significance; no effect on catalytic activity; dbSNP:rs374535734
D375 (≠ S325) to Y: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs267606759
R386 (= R336) binding (R)-2-hydroxyglutarate; binding (R)-lactate; binding (R)-malate; mutation to A: Loss of catalytic activity.
T390 (≠ S340) binding (R)-2-hydroxyglutarate; binding (R)-malate; mutation to A: Significantly reduced catalytic activity.
V399 (≠ S349) to M: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs746519212
K401 (= K351) binding (R)-2-hydroxyglutarate; binding (R)-malate; mutation to A: Loss of catalytic activity.
R419 (≠ E369) to H: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs199908032
A426 (= A379) to T: in D2HGA1; uncertain significance; no effect on catalytic activity; dbSNP:rs146578303
H434 (= H386) binding Zn(2+); mutation to A: Loss of catalytic activity.
G436 (= G388) to V: slight reduction in catalytic activity
N439 (= N391) to D: in D2HGA1; uncertain significance; mild phenotype; moderate reduction in catalytic activity; dbSNP:rs121434362
H441 (= H393) binding Zn(2+); mutation to A: Loss of catalytic activity.
N443 (= N395) binding (R)-2-hydroxyglutarate; mutation to A: Significantly reduced catalytic activity.
V444 (≠ I396) to A: in D2HGA1; uncertain significance; severe phenotype; significant reduction in catalytic activity; dbSNP:rs121434360
A446 (≠ Q398) to V: in D2HGA1; uncertain significance; moderate reduction in catalytic activity; dbSNP:rs746956176
E475 (= E432) binding Zn(2+); mutation to A: Loss of catalytic activity.
H476 (= H433) binding (R)-2-hydroxyglutarate; binding (R)-lactate; binding (R)-malate; mutation to A: Loss of catalytic activity.
G477 (= G434) to R: in D2HGA1; uncertain significance; almost complete loss of catalytic activity; dbSNP:rs1453924640

Sites not aligning to the query:

15 R → G: in dbSNP:rs4675887
169:521 natural variant: Missing (in D2HGA1; uncertain significance)
400:521 natural variant: Missing (in D2HGA1; uncertain significance; complete loss of catalytic activity)

P9WIT1 Uncharacterized FAD-linked oxidoreductase Rv2280; EC 1.-.-.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
33% identity, 89% coverage: 50:474/476 of query aligns to 45:456/459 of P9WIT1

query
sites
P9WIT1

K

K

P

P

G

A

S

T

V

P

E

E

V

V

S

A

S

Q

I

L

L

L

K

K

L

A

A

A

S

S

E

E

T

N

G

G

A

V

A

P

I

V

V

T

P

A

Q

R

T

G

G

S

N

G