SitesBLAST

R52 (≠ E51) mutation to H: Shows increased expression and forms larger colonies.
H86 (≠ Y84) mutation to R: Can be bound and inactivated by MtfA.
F136 (= F137) mutation to A: Decreases association with PtsG EIIB domain.
H247 (= H247) binding Zn(2+)
C257 (= C257) binding Zn(2+); mutation to A: Strongly reduced activity; when associated with A-259.; mutation to S: Strongly reduced activity; when associated with S-259.
C259 (= C259) binding Zn(2+); mutation to A: Strongly reduced activity; when associated with A-257.; mutation to S: Strongly reduced activity; when associated with S-257.
C264 (= C264) binding Zn(2+)
R306 (= R300) mutation to G: Forms dimers but not tetramers; when associated with G-310.

Sites not aligning to the query:

310 L→G: Forms dimers but not tetramers; when associated with G-306.

1z6rA Crystal structure of mlc from escherichia coli (see paper)
26% identity, 71% coverage: 11:301/408 of query aligns to 1:283/382 of 1z6rA

query
sites
1z6rA

Q

Q

A

I

K

K

S

Q

H

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N

N

R

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R

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A

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H

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G

binding zinc ion: H223 (= H247), C233 (= C257), C235 (= C259), C240 (= C264)

5f7qE Rok repressor lmo0178 from listeria monocytogenes bound to operator (see paper)
25% identity, 64% coverage: 8:270/408 of query aligns to 7:266/396 of 5f7qE

query
sites
5f7qE

N

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H
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H

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S

binding zinc ion: H243 (= H247), C253 (= C257), C255 (= C259), C260 (= C264)
binding : K8 (≠ L9), K12 (= K13), N15 (= N16), T32 (≠ A33), S43 (≠ Q44), T44 (= T45), T67 (≠ A68), G68 (≠ R69), G68 (≠ R69), G69 (= G70), G69 (= G70), R70 (≠ Q71), R70 (≠ Q71), R71 (≠ P72), A72 (≠ I73), K73 (≠ I74)

Sites not aligning to the query:

binding : 5

6jdbA Crystal structure of n-acetyl mannosmaine kinase in complex with mannac-6p and adp from haemophilus influenzae
27% identity, 57% coverage: 113:345/408 of query aligns to 29:246/290 of 6jdbA

query
sites
6jdbA

H

H

V

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R

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R

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x
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K

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E

binding adenosine-5'-diphosphate: S129 (≠ A219), T130 (≠ G220), P195 (= P288), K196 (≠ D289), S241 (= S334)
binding 2-acetamido-2-deoxy-6-O-phosphono-alpha-D-mannopyranose: T62 (≠ P152), G63 (= G153), A72 (≠ S162), L73 (= L163), N74 (≠ S164), N77 (= N167), N102 (= N192), D103 (= D193), S129 (≠ A219), T130 (≠ G220), H152 (≠ E244), H155 (= H247), E174 (≠ D266)
binding zinc ion: H155 (= H247), C165 (= C257), C167 (= C259), C172 (= C264)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 12

2qm1B Crystal structure of glucokinase from enterococcus faecalis
24% identity, 74% coverage: 86:387/408 of query aligns to 9:324/325 of 2qm1B

query
sites
2qm1B

I

I

G

G

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N

A

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A

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S

E

K

binding magnesium ion: N116 (= N192), N119 (≠ T195), G148 (≠ A226)
binding zinc ion: H169 (= H247), C179 (= C257), C181 (= C259), C186 (= C264)

6jdcA Crystal structure of n-acetyl mannosmaine kinase in complex with mannac from haemophilus influenzae
26% identity, 64% coverage: 86:345/408 of query aligns to 4:226/269 of 6jdcA

query
sites
6jdcA

I

L

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A

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D

-

R

-

D

-

R

-

L

-

G

-

V

V

G

A

I

V

A

A

L

S

P

T

G

G

R

I

Y

I

A

N

D

N

G

G

G

I

V

L

T

S

S

A

L

L

S

N

P

P

L

K

N

N

L

L

P

G

G

G

W

L

Q

A

N

E

F

F

P

P

V

L

A

K

R

A

E

S

L

I

E

A

R

K

L

H

I

T

G

D

L

K

P

P

V

I

L

G

V

L

E

L

N

N

D

D

A

A

T

Q

A

A

G

A

A

T

I

Y

G

A

E

E

R

Y

L

Q

Y

L

G

Q

V

N

A

F

R

E

G

Q

L

V

G

S

S

N

F

F

V

V

Y

F

L

I

F

T

L

V

A

S

G

T

D

-

G

-

G

G

I

V

G

G

A

G

G

G

M

I

F

V

L

L

D

N

G

Q

H

I

L

L

Y

Q

K

T

G

G

S

S

R

R

A

G

N

I

A

A

G

G

E

H

I

I

G

G

H
|
H

I

T

I

L

V

A

E

D

P

P

H

N

G

G

R

A

L

I

C
|
C

T

G

C
|
C

G

G

K

R

R

R

G

G

C
|
C

L

V

D

E

R

A

Y

I

V

A

S

S

P

G

S

R

V

A

A

-

Y

-

E

-

C

-

L

-

G

-

I

-

E

-

D

-

A

-

Q

-

E

-

L

-

D

-

P

-

D

-

L

I

D

E

A

A

M

V

I

S

A

S

A

A

N

T

S

A

R

-

G

-

L

-

Q

-

T

-

W

L

L

V

E

E

Q

R

A

S

V

A

Q

K

P

A

L

I

R

A

Q

N

T

L

I

I

D

A

F

D

L

L

E

V

L

I

A

S

F

L

D

D

P

I

Q

Q

T

K

I

I

V

A

L

I

G

G

S

S

A

-

P

-

S

-

L

-

M

-

T

V

G

G

L

L

A

A

E

E

binding zinc ion: H156 (= H247), C166 (= C257), C168 (= C259), C173 (= C264)

6jdoA Crystal structure of n-acetyl mannosmaine kinase with amp-pnp from pasteurella multocida
28% identity, 57% coverage: 147:380/408 of query aligns to 58:283/293 of 6jdoA

query
sites
6jdoA

V

V

G

A

I

V

A

A

L

S

P

T

G

G

R

I

Y

I

A

N

D

H

G

G

G

V

V

L

T

T

S

A

L

L

S

N

P

P

L

K

N

N

L

L

P

G

G

G

W

L

Q

A

N

E

F

F

P

P

V

L

A

K

R

E

E

S

L

I

E

A

R

R

L

H

I

T

G

D

L

K

P

P

V

I

L

G

V

L

E

L

N

N

D

D

A

V

T

Q

A

A

G

A

A

A

I

C

G

A

E

E

R

Y

L

K

Y

D

G

E

V

D

A

K

R

N

G

A

L

V

G

Q

S

N

F

F

V

V

Y

F

L

I

F

T

L

V

A
x
S

G
x
T

D

-

G

-

G

G

I

V

G

G

A

G

G

G

M

I

F

I

L

L

D

E

G

R

H

R

L

L

Y

L

K

T

G

E

S

P

R

N

A

G

N

V

A

A

G

G

E

H

I

I

G

G

H
|
H

I

T

I

L

V

A

E

D

P

P

H

N

G

G

R

P

L

V

C
|
C

T

G

C
|
C

G

G

K
x
R

R

V

G

G

C

C

L

V

D

E

R

A

Y

-

V

V

S

A

P

A

S
x
G

V

R

A

A

Y

I

E

E

C

A

L

V

G

S

I

S

E

Q

D

W

A

N

Q

P

E

P

L

C

D

T

P
|
P

D

K

D

Q

L

A

D
x
F

A

E

M

L

I

F

A

R

A

K

N

N

S

D

R

E

G

K

L

A

Q

T

T

A

W

L

L

I

E

Q

Q

R

A

S

V

A

Q

S

P

A

L

I

R

A

Q

N

T

L

I

I

D

A

F

D

L

L

E

V

L

I

A

G

F

L

D

D

P

V

Q

Q

T

K

I

V

V

V

L

V

G

G

S
|
S

A

-

P

-

S

-

L

-

M

-

T

V

G

G

L

L

A

A

E

E

R

G

V

Y

E

L

P

P

L

L

-

V

H

K

I

Q

P

Y

V

L

N

N

P

T

M

M

E

P

E

H

R

F

K

Y

I

H

P

C

R

T

L

V

M

E

V

Q

G

A

A

R

T

H

G

G

K

Q

D

D

T

A

A

G

I

L

L

L

G

G

A

A

binding phosphoaminophosphonic acid-adenylate ester: S130 (≠ A219), T131 (≠ G220), G180 (≠ S272), P196 (= P288), F200 (≠ D292), S242 (= S334)
binding calcium ion: H156 (= H247), C166 (= C257), C168 (= C259), R170 (≠ K261)

6jdhA Crystal structure of n-acetyl mannosmaine kinase from pasteurella multocida
28% identity, 57% coverage: 147:380/408 of query aligns to 58:283/293 of 6jdhA

query
sites
6jdhA

V

V

G

A

I

V

A

A

L

S

P

T

G

G

R

I

Y

I

A

N

D

H

G

G

G

V

V

L

T

T

S

A

L

L

S

N

P

P

L

K

N

N

L

L

P

G

G

G

W

L

Q

A

N

E

F

F

P

P

V

L

A

K

R

E

E

S

L

I

E

A

R

R

L

H

I

T

G

D

L

K

P

P

V

I

L

G

V

L

E

L

N

N

D

D

A

V

T

Q

A

A

G

A

A

A

I

C

G

A

E

E

R

Y

L

K

Y

D

G

E

V

D

A

K

R

N

G

A

L

V

G

Q

S

N

F

F

V

V

Y

F

L

I

F

T

L

V

A

S

G

T

D

-

G

-

G

G

I

V

G

G

A

G

G

G

M

I

F

I

L

L

D

E

G

R

H

R

L

L

Y

L

K

T

G

E

S

P

R

N

A

G

N

V

A

A

G

G

E

H

I

I

G

G

H
|
H

I

T

I

L

V

A

E

D

P

P

H

N

G

G

R

P

L

V

C
|
C

T

G

C
|
C

G

G

K

R

R

V

G

G

C
|
C

L

V

D

E

R

A

Y

-

V

V

S

A

P

A

S

G

V

R

A

A

Y

I

E

E

C

A

L

V

G

S

I

S

E

Q

D

W

A

N

Q

P

E

P

L

C

D

T

P

P

D

K

D

Q

L

A

D

F

A

E

M

L

I

F

A

R

A

K

N

N

S

D

R

E

G

K

L

A

Q

T

T

A

W

L

L

I

E

Q

Q

R

A

S

V

A

Q

S

P

A

L

I

R

A

Q

N

T

L

I

I

D

A

F

D

L

L

E

V

L

I

A

G

F

L

D

D

P

V

Q

Q

T

K

I

V

V

V

L

V

G

G

S

S

A

-

P

-

S

-

L

-

M

-

T

V

G

G

L

L

A

A

E

E

R

G

V

Y

E

L

P

P

L

L

-

V

H

K

I

Q

P

Y

V

L

N

N

P

T

M

M

E

P

E

H

R

F

K

Y

I

H

P

C

R

T

L

V

M

E

V

Q

G

A

A

R

T

H

G

G

K

Q

D

D

T

A

A

G

I

L

L

L

G

G

A

A

binding zinc ion: H156 (= H247), C166 (= C257), C168 (= C259), C173 (= C264)

4db3A 1.95 angstrom resolution crystal structure of n-acetyl-d-glucosamine kinase from vibrio vulnificus.
26% identity, 66% coverage: 117:384/408 of query aligns to 38:309/311 of 4db3A

query
sites
4db3A

P

P

G

T

P

P

T

T

E

D

A

D

M

Y

P

P

V

L

F

L

-

L

-

E

-

T

-

I

A

A

A

G

I

L

V

V

R

A

D

K

L

Y

Q

D

E

Q

A

E

F

F

S

A

F

C

D

E

R

G

D

K

R

-

L

-

G

-

V

I

G

G

I

L

A

G

L

L

P

P

G

G

R

M

Y

-

A

E

D

D

G

A

G

D

V

D

T

A

S

T

L

V

S

L

P

T

L

V

N

N

L

V

P

P

G

A

W

A

Q

K

N

G

F

K

P

P

V

L

A

R

R

A

E

D

L

L

E

E

R

A

L

K

I

I

G

G

L

R

P

S

V

V

L

K

V

I

E

E

N

N

D

D

A

A

T

N

A

C

G

F

A

A

I

L

G

S

E

E

R

A

L

W

Y

D

G

E

V

E

A

L

R

Q

G

D

L

A

G

P

S

S

F

V

V

M

Y

G

L

L

F

I

L

L

A

G

G

-

D

-

G

T

G

G

I

F

G

G

A

G

G

G

M

L

F

I

L

Y

D

E

G

G

H

K

L

V

Y

F

K

S

G

G

S

R

R

N

A

N

N

V

A

A

G

G

E

E

I

L

G

G

H
|
H

I

M

I

R

V

L

E

P

P

L

H

D

G

A

R

W

L

F

-

H

-

L

-

G

-

D

-

N

-

A

-

P

-

L

-

G

C
|
C

T

G

C
|
C

G

G

K

K

R

K

G

G

C
|
C

L

L

D

D

R

S

Y

Y

V

L

S

S

P

G

-

R

-

G

-

F

S

E

V

L

A

L

Y

Y

E

A

C

H

L

Y

G

Y

I

G

E

E

D

E

A

K

Q

K

E

A

L

I

D

D

P

-

D

-

L

-

D

-

A

-

M

I

I

I

A

K

A

A

N

N

S

A

R

A

G

G

L

-

Q

-

T

-

W

-

L

D

E

E

Q

K

A

A

V

A

Q

E

P

H

L

V

R

E

Q

R

T

F

I

M

D

E

F

L

L

L

E

A

L

I

A

C

F

F

-

G

-

N

-

I

-

F

-

T

-

A

-

N

D

D

P

P

Q

H

T

V

I

V

V

A

L

L

G

G

S

G

A

L

P

-

S

S

L

N

M

F

T

E

G

L

L

I

A

Y

E

E

R

E

V

M

E

-

P

P

L

K

H

R

I

V

P

P

V

K

N

Y

P

L

M

L

E

S

E

V

R

A

K

K

I

C

P

P

R

K

L

I

M

I

V

K

G

A

A

K

T

H

G

G

K

D

D

S

T

G

A

G

I

V

L

R

G

G

A

A

A

F

L

L

P

N

I

I

binding zinc ion: H165 (= H247), C185 (= C257), C187 (= C259), C192 (= C264)

2aa4A Crystal structure of escherichia coli putative n-acetylmannosamine kinase, new york structural genomics consortium
31% identity, 49% coverage: 147:345/408 of query aligns to 59:247/289 of 2aa4A

query
sites
2aa4A

V

V

G

A

I

I

A

A

L

S

P

T

G

G

R

I

Y

I

A

R

D

D

G

G

G

S

V

L

T

L

S

A

L

L

S

N

P

P

L

H

N

N

L

L

P

G

G

G

W

L

Q

L

N

H

F

F

P

P

V

L

A

V

R

K

E

T

L

L

E

E

R

Q

L

L

I

T

G

N

L

L

P

P

V

T

L

I

V

A

E

I

N

N

D

D

A

A

T

Q

A

A

G

A

A

A

I

W

G

A

E

E

R

-

L

-

Y

-

G

-

V

-

A

-

R

-

G

-

L

-

G

-

S

-

F

-

V

-

Y

-

L

-

F

F

L

Q

A

A

G

L

D

D

G

G

G

D

I

I

G

T

A

D

G

M

M

V

F

F

L

I

D

T

G

V

-

S

-

T

-

G

-

V

-

G

-

V

-

S

-

G

-

C

H

K

L

L

Y

L

K

T

G

G

S

P

R

G

A

G

N

L

A

A

G

G

E

H

I

I

G

G

H
|
H

I

T

I

L

V

A

E

D

P

P

H

H

G

G

R

P

L

V

C
|
C

T

G

C
|
C

G

G

K

R

R

T

G

G

C
|
C

L

V

D

E

R

A

Y

I

V

A

S

S

P

G

S

R

V

-

A

-

Y

-

E

-

C

-

L

-

G

G

I

I

E

A

D

A

A

A

Q

A

-

Q

-

G

E

E

L

L

D

A

P

G

D

A

D

D

L

A

D

K

A

T

M

I

I

F

A

T

A

R

N

A

S

G

R

Q

G

G

-

D

-

E

-

Q

L

A

Q

Q

T

Q

W

L

L

I

E

H

Q

R

A

S

V

A

Q

R

P

T

L

L

R

A

Q

R

T

L

I

I

D

A

F

D

L

I

E

K

L

A

A

T

F

T

D

D

P

C

Q

Q

T

C

I

V

V

V

L

V

G

G

S

S

A

-

P

-

S

-

L

-

M

-

T

V

G

G

L

L

A

A

E

E

binding zinc ion: H156 (= H247), C166 (= C257), C168 (= C259), C173 (= C264)

P45425 N-acetylmannosamine kinase; ManNAc kinase; N-acetyl-D-mannosamine kinase; EC 2.7.1.60 from Escherichia coli (strain K12) (see paper)
31% identity, 49% coverage: 147:345/408 of query aligns to 59:247/291 of P45425

query
sites
P45425

V

V

G

A

I

I

A

A

L

S

P

T

G

G

R

I

Y

I

A

R

D

D

G

G

G

S

V

L

T

L

S

A

L

L

S

N

P

P

L

H

N

N

L

L

P

G

G

G

W

L

Q
x
L

N

H

F

F

P

P

V

L

A

V

R

K

E

T

L

L

E

E

R

Q

L

L

I

T

G

N

L

L

P

P

V

T

L

I

V

A

E

I

N

N

D

D

A

A

T

Q

A

A

G

A

A

A

I

W

G

A

E

E

R

-

L

-

Y

-

G

-

V

-

A

-

R

-

G

-

L

-

G

-

S

-

F

-

V

-

Y

-

L

-

F

F

L

Q

A

A

G

L

D

D

G

G

G

D

I

I

G

T

A

D

G

M

M

V

F

F

L

I

D

T

G

V

-

S

-

T

-

G

-

V

-

G

-

V

-
x
V

-

S

-

G

-

C

H

K

L

L

Y

L

K

T

G

G

S

P

R

G

A

G

N

L

A

A

G

G

E

H

I

I

G

G

H

H

I

T

I

L

V

A

E

D

P

P

H

H

G

G

R

P

L

V

C

C

T

G

C

C

G

G

K

R

R

T

G

G

C

C

L

V

D

E

R

A

Y

I

V

A

S

S

P

G

S

R

V

-

A

-

Y

-

E

-

C

-

L

-

G

G

I

I

E

A

D

A

A

A

Q

A

-

Q

-

G

E

E

L

L

D

A

P

G

D

A

D

D

L

A

D

K

A

T

M

I

I

F

A

T

A

R

N

A

S

G

R

Q

G

G

-

D

-

E

-

Q

L

A

Q

Q

T

Q

W

L

L

I

E

H

Q

R

A

S

V

A

Q

R

P

T

L

L

R

A

Q

R

T

L

I

I

D

A

F

D

L

I

E

K

L

A

A

T

F

T

D

D

P

C

Q

Q

T

C

I

V

V

V

L

V

G

G

S

S

A

-

P

-

S

-

L

-

M

-

T

V

G

G

L

L

A

A

E

E

L84 (≠ Q172) mutation to P: 12-fold increase in catalytic efficiency for glucose phosphorylation. 2-fold decrease in catalytic efficiency for N-acetylmannosamine phosphorylation.
V138 (vs. gap) mutation to M: 6-fold increase in catalytic efficiency for glucose phosphorylation. No change in catalytic efficiency for N-acetylmannosamine phosphorylation.

7p7wBBB Ubiquitin-like protein SMT3,N-acetyl-D-glucosamine kinase
27% identity, 58% coverage: 147:384/408 of query aligns to 64:306/306 of 7p7wBBB

query
sites
7p7wBBB

V

V

G

G

I

I

A

G

L

I

P

P

G

G

R

-

Y

I

A

A

D

D

G

V

G

E

V

T

T

G

S

K

L

L

S

L

P

T

L

S

N

N

L

I

P

P

G

A

W

A

Q

M

N

G

F

H

P

T

V

L

A

Q

R

R

E

D

L

L

E

E

R

E

L

R

I

L

G

Q

L

R

P

P

V

V

L

K

V

I

E

E

N

N

D

D

A

A

T

N

A

C

G

F

A

A

I

L

G

S

E

E

R

A

L

W

Y

D

G

E

V

D

A

L

R

R

G

G

L

E

G

P

S

S

F

V

V

L

Y

G

L

L

F

I

L

L

A
x
G

G

-

D

-

G
x
T

G

G

I

V

G

G

A

G

G

G

M

L

F

I

L

F

D

N

G

G

H

K

L

V

Y

H

K

S

G

G

S

R

R

A

A

N

N

I

A

A

G

G

E

E

I

I

G

G

H
|
H

I

T

I

R

V

L

E

-

P

P

H

Y

G

D

R

A

L

L

-

K

-

L

-

G

-

M

-

E

-

N

-

A

-

P

-

I

-

F

-

P

C
|
C

T

G

C
|
C

G

K

K

N

R

S

G

G

C
|
C

L

I

D

D

R

N

Y

Y

V

L

S

S

P
x
G

S

R

V

G

A

F

Y
x
E

E

Q

C

L

L

Y

G

D

I

H

E

Y

D

F

A

S

Q

E

E

K

L

L

D

S

P

A

D

P

D

E

L

I

D

I

A

A

M

H

I

Y

A

E

A

Q

N

G

S

E

R

R

G

R

L

A

Q

V

T

Q

W

H

L

V

E

E

Q

R

A

F

V

M

Q

E

P

L

L

L

R

A

Q

I

T

C

I

L

D

A

F

N

L

I

E

F

L

T

A

C

F

L

D

D

P

P

Q

H

T

V

I

V

V

V

L

L

G

G

G
|
G

S
x
G

A

L

P

S

P
x
N

S

-

L

-

M

-

T

F

G

E

L

L

A

I

E

Y

R

Q

V

E

E

L

P

P

L

K

H

R

I

L

P

P

V

A

N

H

P

L

M

L

E

H

E

V

R

A

K

K

I

L

P

P

R

K

L

I

M

I

V

K

G

A

A

R

T

H

G

G

K

D

D

A

T

G

A

G

I

V

L

R

G

G

A

A

A

F

L

L

P

N

I

L

binding adenosine-5'-diphosphate: G135 (≠ A219), T136 (≠ G222), G196 (≠ P271), E200 (≠ Y275), G258 (= G333), G259 (≠ S334), N262 (≠ P337)
binding zinc ion: H161 (= H247), C182 (= C257), C184 (= C259), C189 (= C264)

7p9lAAA Ubiquitin-like protein SMT3,N-acetyl-D-glucosamine kinase
27% identity, 58% coverage: 147:384/408 of query aligns to 61:303/303 of 7p9lAAA

query
sites
7p9lAAA

V

V

G

G

I

I

A

G

L

I

P
|
P

G
|
G

R

-

Y

I

A

A

D

D

G

V

G

E

V

T

T

G

S

K

L

L

S

L

P

T

L
x
S

N

N

L

I

P

P

G

A

W

A

Q

M

N

G

F

H

P

T

V

L

A

Q

R

R

E

D

L

L

E

E

R

E

L

R

I

L

G

Q

L

R

P

P

V

V

L

K

V

I

E

E

N
|
N

D
|
D

A

A

T

N

A

C

G

F

A

A

I

L

G

S

E

E

R

A

L

W

Y

D

G

E

V

D

A

L

R

R

G

G

L

E

G

P

S

S

F

V

V

L

Y

G

L

L

F

I

L

L

A
x
G

G

-

D

-

G
x
T

G
|
G

I
x
V

G
|
G

A

G

G

G

M

L

F

I

L

F

D

N

G

G

H

K

L

V

Y

H

K

S

G

G

S

R

R

A

A

N

N

I

A

A

G

G

E
|
E

I

I

G

G

H
|
H

I

T

I

R

V

L

E

-

P

P

H

Y

G

D

R

A

L

L

-

K

-

L

-

G

-

M

-

E

-

N

-

A

-

P

-

I

-

F

-

P

C
|
C

T

G

C
|
C

G

K

K

N

R

S

G

G

C
|
C

L

I

D
|
D

R

N

Y

Y

V

L

S

S

P

G

S

R

V

G

A

F

Y

E

E

Q

C

L

L

Y

G

D

I

H

E

Y

D

F

A

S

Q

E

E

K

L

L

D

S

P

A

D

P

D
x
E

L

I

D

I

A

A

M
x
H

I

Y

A

E

A

Q

N

G

S

E

R

R

G

R

L

A

Q

V

T

Q

W

H

L

V

E

E

Q

R

A

F

V

M

Q

E

P

L

L

L

R

A

Q

I

T

C

I

L

D

A

F

N

L

I

E

F

L

T

A

C

F

L

D

D

P

P

Q

H

T

V

I

V

V

V

L

L

G

G

S

G

A

L

P

S

P

N

S

-

L

-

M

-

T

F

G

E

L

L

A

I

E

Y

R

Q

V

E

E

L

P

P

L

K

H

R

I

L

P

P

V

A

N

H

P

L

M

L

E

H

E

V

R

A

K

K

I

L

P

P

R

K

L

I

M

I

V

K

G

A

A

R

T

H

G

G

K

D

D

A

T

G

A

G

I

V

L

R

G

G

A

A

A

F

L

L

P

N

I

L

binding 2-acetamido-2-deoxy-6-O-phosphono-beta-D-glucopyranose: P66 (= P152), G67 (= G153), S79 (≠ L166), N105 (= N192), D106 (= D193), G132 (≠ A219), T133 (≠ G222), G134 (= G223), V135 (≠ I224), G136 (= G225), E155 (= E244), H158 (= H247), D188 (= D266)
binding zinc ion: H158 (= H247), C179 (= C257), C181 (= C259), C186 (= C264), E212 (≠ D290), H216 (≠ M294)

7p9pAAA Ubiquitin-like protein SMT3,N-acetyl-D-glucosamine kinase
26% identity, 65% coverage: 119:384/408 of query aligns to 34:304/304 of 7p9pAAA

query
sites
7p9pAAA

P

P

T

T

E

E

A

S

M

Y

P

A

V

A

F

F

A

L

A

D

I

A

V

I

R

V

D

T

L

L

Q

V

E

N

A

N

F

A

S

D

F

A

D

E

R

F

D

G

R

V

L

K

L

G

G

T

V

V

G

G

I

I

A

G

L

I

P

P

G

G

R

-

Y

I

A

A

D

D

G

V

G

E

V

T

T

G

S

K

L

L

S

L

P

T

L

S

N

N

L

I

P

P

G

A

W

A

Q

M

N

G

F

H

P

T

V

L

A

Q

R

R

E

D

L

L

E

E

R

E

L

R

I

L

G

Q

L

R

P

P

V

V

L

K

V

I

E

E

N

N

D

D

A

A

T

N

A

C

G

F

A

A

I

L

G

S

E

E

R

A

L

W

Y

D

G

E

V

D

A

L

R

R

G

G

L

E

G

P

S

S

F

V

V

L

Y

G

L

L

F

I

L

L

A
x
G

G

-

D

-

G
x
T

G

G

I

V

G

G

A

G

G

G

M

L

F

I

L

F

D

N

G

G

H

K

L

V

Y

H

K

S

G

G

S

R

R

A

A

N

N

I

A

A

G

G

E

E

I

I

G

G

H
|
H

I

T

I

R

V

L

E

-

P

P

H

Y

G

D

R

A

L

L

-

K

-

L

-

G

-

M

-

E

-

N

-

A

-

P

-

I

-

F

-

P

C
|
C

T

G

C
|
C

G

K

K

N

R

S

G

G

C
|
C

L

I

D

D

R

N

Y

Y

V

L

S

S

P
x
G

S

R

V

G

A

F

Y
x
E

E

Q

C

L

L

Y

G

D

I

H

E

Y

D

F

A

S

Q

E

E

K

L

L

D

S

P
x
A

D

P

D
x
E

L

I

D

I

A

A

M
x
H

I

Y

A

E

A

Q

N

G

S

E

R

R

G

R

L

A

Q

V

T

Q

W

H

L

V

E

E

Q

R

A

F

V

M

Q

E

P

L

L

L

R

A

Q

I

T

C

I

L

D

A

F

N

L

I

E

F

L

T

A

C

F

L

D

D

P

P

Q

H

T

V

I

V

V

V

L

L

G

G

G
|
G

S
x
G

A

L

P

S

P
x
N

S

-

L

-

M

-

T

F

G

E

L

L

A

I

E

Y

R

Q

V

E

E

L

P

P

L

K

H

R

I

L

P

P

V

A

N

H

P

L

M

L

E

H

E

V

R

A

K

K

I

L

P

P

R

K

L

I

M

I

V

K

G

A

A

R

T

H

G

G

K

D

D

A

T

G

A

G

I

V

L

R

G

G

A

A

A

F

L

L

P

N

I

L

binding phosphoaminophosphonic acid-adenylate ester: G133 (≠ A219), T134 (≠ G222), G194 (≠ P271), E198 (≠ Y275), A211 (≠ P288), G256 (= G333), G257 (≠ S334), N260 (≠ P337)
binding zinc ion: H159 (= H247), C180 (= C257), C182 (= C259), C187 (= C264), E213 (≠ D290), H217 (≠ M294)

Sites not aligning to the query:

binding phosphoaminophosphonic acid-adenylate ester: 11, 12, 13

3vovB Crystal structure of rok hexokinase from thermus thermophilus (see paper)
34% identity, 39% coverage: 167:326/408 of query aligns to 79:236/298 of 3vovB

query
sites
3vovB

N

N

L

I

P

P

G

G

W

V

Q

Q

N

D

F

F

P

P

V

I

A

R

R

R

E

I

L

L

E

E

R

E

L

A

I

T

G

G

L

R

P

P

V

V

L

F

V

L

E

E

N

N

D

D

A

A

T

N

A

A

G

A

A

A

I

L

G

A

E

E

R

H

L

H

Y

L

G

G

V

A

A

A

R

Q

G

G

L

E

G

E

S

S

F

S

V

L

Y

Y

L

L

F

T

L

V

A

S

G

T

D

-

G

-

G

G

I

I

G

G

A

G

G

G

M

V

F

V

L

L

D

G

G

G

H

R

L

V

Y

L

K

R

G

G

S

E

R

R

A

G

N

Q

A

G

G

G

E

E

I

L

G

G

H
|
H

I

L

I

T

V

L

E

L

P

P

H

G

G

G

R

P

L

A

C
|
C

T

G

C
|
C

G

G

K

L

R

E

G

G

C
|
C

L

L

D

E

R

A

Y

L

V

A

S

A

P

G

S

R

V

A

A

L

Y

E

E

R

C

D

L

A

G

T

I

Y

E

A

D

F

A

Q

Q

R

E

P

L

V

D

D

P

T

D

R

D

E

L

L

D

F

A

R

M

L

I

F

A

Q

A

A

N

G

S

D

R

P

G

K

L

A

Q

E

T

R

W

L

L

V

E

L

Q

Q

A

A

V

A

Q

R

P

Y

L

V

R

G

Q

I

T

G

I

L

D

A

F

S

L

L

E

V

L

K

A

A

F

F

D

D

P

P

binding zinc ion: H157 (= H247), C167 (= C257), C169 (= C259), C174 (= C264)

5f7rA Rok repressor lmo0178 from listeria monocytogenes bound to inducer (see paper)
26% identity, 46% coverage: 84:270/408 of query aligns to 2:185/306 of 5f7rA

query
sites
5f7rA

Y

F

S

V

I

V

G

S

L

V

E
x
K

L

V

G

E

R
x
E

Q

E

R

Q

A

L

G

F

V

A

L

L

T

T

D

D

L

L

S

N

G

A

T

E

V

I

C

I

A

E

R

N

I

T

D

S

R

I

-

P

H

F

V

S

D

S

R

E

P

K

G

K

P

P

T

E

E

E

A

A

M

I

P

E

V

L

F

I

A

A

A

K

I

N

V

V

R

K

D

K

L

M

Q

-

E

-

A

C

F

G

S

N

F

R

D

D

R

M

D

N

R

H

L

L

G

G

V

V

G

G

I

I

A

A

L

I

P

S

G
|
G

R

L

Y

V

A

N

D

R

G

K

V

G

T

T

S

V

L

I

S

R

P

S

L

T

N

M

L

L

P

-

G

G

W

W

Q

E

N

N

F

V

P

A

V

L

A

E

R

A

E

M

L

L

E

H

-

A

R

H

L

F

I

P

G

D

L

I

P

P

V

V

L

Y

V

V

E

D

N

K

D
x
N

A

I

T

N

A

C

G

Y

A

T

I

L

G

A

E

E

R

L

L

W

Y

L

G

G

V

E

A

G

R

K

G

Q

L

S

G

N

S

N

F

F

V

A

Y

T

L

V

F
x
S

L
x
V

A

G

G

A

D

-

G

-

G
|
G

I
x
L

G
|
G

A

L

G

S

M

V

F

V

L

I

D

N

G

R

H

Q

L

I

Y

Y

K

Y

G

G

S

A

R

Q

A

G

N

G

A

A

G

G

E
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E

I

F

G

G

H
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H

I

T

V

I

E

Q

P

P

H

G

G

G

R

Y

L

K

C
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C

T

H

C
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C

G

G

K

Q

R

K

G

G

C
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C

L

L

D
x
E

R

M

Y

Y

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A

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S

binding alpha-D-glucopyranose: K7 (≠ E89), E10 (≠ R92), G70 (= G153), N110 (≠ D193), N110 (≠ D193), S134 (≠ F217), V135 (≠ L218), G138 (= G223), L139 (≠ I224), G140 (= G225), E159 (= E244), H162 (= H247), E181 (≠ D266)
binding zinc ion: H162 (= H247), C172 (= C257), C174 (= C259), C179 (= C264)

Sites not aligning to the query:

binding alpha-D-glucopyranose: 253, 293

Q9Y223 Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase; UDP-GlcNAc-2-epimerase/ManAc kinase; EC 3.2.1.183; EC 2.7.1.60 from Homo sapiens (Human) (see 26 papers)
35% identity, 31% coverage: 143:270/408 of query aligns to 466:592/722 of Q9Y223

query
sites
Q9Y223

R
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R

L
x
I

L
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L

G
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G

V
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V

G
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G

I
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I

A
x
S

L
x
T

P
x
G

G
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G

R
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R

Y
x
V

A
x
N

-
x
P

D
x
R

G
x
E

G
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G

V
x
I

T
x
V

S
x
L

L
x
H

S
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S

P
x
T

L
x
K

N
x
L

L
x
I

P
x
Q

G
x
E

W
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W

Q
x
N

N
x
S

F
x
V

P
x
D

V
x
L

A
x
R

R
x
T

E
x
P

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L

E
x
S

R
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D

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T

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x
L

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x
H

L
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L

P
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P

V
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V

L
x
W

V
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V

E
x
D

N
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N

D
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D

A
x
G

T
x
N

A
x
C

G
x
A

A
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A

I
x
L

G
x
A

E
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E

R
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R

L
x
K

Y
x
F

G
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G

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x
Q

A
x
G

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x
K

G
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G

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L

G
x
E

S
x
N

F
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F

V
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V

Y
x
T

L
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L

F
x
I

L
x
T

A
x
G

G
x
T

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x
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x
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G

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G

A
x
I

G
x
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M
x
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F

-

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-

D
x
Q

G
x
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H
x
E

L
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L

Y
x
I

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x
H

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G

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S

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x
S

A
x
F

N
x
C

A
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A

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x
A

E
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E

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x
L

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G

H
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H

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V

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L

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x
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G

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P

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D

C
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C

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S

C
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C

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G

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x
S

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H

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G

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C

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Y

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S

I472 (= I149) to T: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; decreased N-acylmannosamine kinase activity corresponding to less than 10% of wild-type activity
G475 (≠ P152) to F: in THC12; likely pathogenic; requires 2 nucleotide substitutions
G476 (= G153) binding an N-acyl-D-mannosamine; binding an N-acyl-D-mannosamine 6-phosphate
R477 (= R154) binding an N-acyl-D-mannosamine; binding an N-acyl-D-mannosamine 6-phosphate
V485 (≠ T161) to R: in THC12; uncertain significance; reduced protein abundance in homozygous patient cells; requires 2 nucleotide substitutions
L486 (≠ S162) to P: in THC12; uncertain significance; dbSNP:rs774867424
T489 (≠ P165) binding an N-acyl-D-mannosamine; binding an N-acyl-D-mannosamine 6-phosphate
N516 (= N192) binding an N-acyl-D-mannosamine; binding an N-acyl-D-mannosamine 6-phosphate
D517 (= D193) active site; binding an N-acyl-D-mannosamine; binding an N-acyl-D-mannosamine 6-phosphate; mutation D->A,N: Loss of N-acylmannosamine kinase activity. Decreased affinity for N-acyl-D-mannosamine. No effect on structure.
N519 (≠ T195) to S: in NM and THC12; likely pathogenic; decreased UDP-N-acetylglucosamine 2-epimerase activity; decreased N-acylmannosamine kinase activity; dbSNP:rs1554658910
A524 (≠ G200) to V: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity corresponding to less than 10% of wild-type activity; decreased N-acylmannosamine kinase activity; impaired homohexamers formation; dbSNP:rs764698870
F528 (≠ Y204) to C: in NM; decreased N-acylmannosamine kinase activity; dbSNP:rs986773986
T544 (≠ G220) to R: in THC12; uncertain significance; reduced protein abundance in homozygous patient cells
G545 (≠ D221) binding an N-acyl-D-mannosamine 6-phosphate
C563 (≠ N241) to Y: in THC12; likely pathogenic; results in severely decreased cell surface sialylation
E566 (= E244) binding an N-acyl-D-mannosamine
H569 (= H247) binding an N-acyl-D-mannosamine; binding an N-acyl-D-mannosamine 6-phosphate; binding Zn(2+)
V572 (= V250) to L: in NM and THC12; likely pathogenic; mildly decreased UDP-N-acetylglucosamine 2-epimerase activity; decreased N-acylmannosamine kinase activity corresponding to less than 10% of wild-type activity; does not affect homohexamers formation; dbSNP:rs121908632
G576 (= G254) to E: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; decreased N-acylmannosamine kinase activity; dbSNP:rs121908625
C579 (= C257) binding Zn(2+)
C581 (= C259) binding Zn(2+)
C586 (= C264) binding Zn(2+)
I587 (≠ L265) to T: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; decreased N-acylmannosamine kinase activity; dbSNP:rs748949603
E588 (≠ D266) binding an N-acyl-D-mannosamine; binding an N-acyl-D-mannosamine 6-phosphate

Sites not aligning to the query:

13 C → S: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; no effect on N-acylmannosamine kinase activity; dbSNP:rs1209266607
19 binding UDP
23 binding UDP
113 binding UDP
132 H → Q: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; impaired homohexamers formation
157 H → Y: in THC12; likely pathogenic
176 D → V: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; no effect on N-acylmannosamine kinase activity; impaired homohexamers formation; dbSNP:rs139425890
177 R → C: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; impaired homohexamers formation; dbSNP:rs539332585
200 I → F: in NM; uncertain significance; dbSNP:rs369328625
206 G → S: in NM; moderate phenotype with unusual involvement of quadriceps; dbSNP:rs766266918
220 binding UDP
253 binding UDP
259 binding CMP-N-acetyl-beta-neuraminate
263 R → L: in SIALURIA; strong reduction of feedback inhibition by CMP-Neu5Ac; dbSNP:rs121908623
266 R → Q: in SIALURIA; abolishes feedback inhibition by CMP-Neu5Ac; dbSNP:rs121908622; R → W: in sialuria; dbSNP:rs121908621
271 binding CMP-N-acetyl-beta-neuraminate
280 binding CMP-N-acetyl-beta-neuraminate
281 binding CMP-N-acetyl-beta-neuraminate
282 binding UDP
301 binding UDP
302 binding UDP
303 C → V: in NM; requires 2 nucleotide substitutions; dbSNP:rs121908633
307 binding UDP
321 binding UDP
331 V → A: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; no effect on N-acylmannosamine kinase activity; impaired homohexamers formation
378 D → Y: in NM; decreased UDP-N-acetylglucosamine 2-epimerase activity; impaired homohexamers formation; dbSNP:rs199877522
413 binding Mg(2+); D → Y: in THC12; likely pathogenic; dbSNP:rs1280775456
416 binding an N-acyl-D-mannosamine 6-phosphate
417 binding ADP; T → M: in THC12; likely pathogenic; dbSNP:rs1554659711
418 binding ADP
420 binding ADP; R → Q: in THC12; likely pathogenic; dbSNP:rs780092539
420:722 natural variant: Missing (in THC12; likely pathogenic)
630 A → T: in NM; decreased N-acylmannosamine kinase activity; does not affect homohexamers formation; dbSNP:rs1382191649
631 A → V: in NM; does not affect homohexamers formation; dbSNP:rs62541771
704 P → R: in THC12; likely pathogenic; the orthologous mutation in mouse embryos results in cerebrospinal hemorrhages and defective angiogenesis; results in loss of cell surface sialylation
708 G → S: in NM and THC12; likely pathogenic; decreased UDP-N-acetylglucosamine 2-epimerase activity; severely decreased N-acylmannosamine kinase activity; dbSNP:rs1554657922
712 M → T: in NM; decreased N-acylmannosamine kinase activity; dbSNP:rs28937594

2yhwA High-resolution crystal structures of n-acetylmannosamine kinase: insights about substrate specificity, activity and inhibitor modelling. (see paper)
33% identity, 37% coverage: 143:291/408 of query aligns to 62:204/309 of 2yhwA

query
sites
2yhwA

R

R

L

I

L

L

G

G

V

V

G

G

I

I

A

S

L

T

P

G

G

G

R

R

Y

V

A

N

-

P

D

R

G

E

G

G

V

I

T

V

S

L

L

H

S

S

P

T

L

K

N

L

L

I

P

Q

G

E

W

W

Q

N

N

S

F

V

P

D

V

L

A

R

R

T

E

P

L

L

E

S

R

D

L

T

I

L

G

H

L

L

P

P

V

V

L

W

V

V

E

D

N
|
N

D

D

A

G

T
x
N

A

C

G

A

A

A

I

L

G

A

E

E

R

R

L

K

Y

F

G

G

V

Q

A

G

R

K

G

G

L

L

G

E

S

N

F

F

V

V

Y

T

L

L

F

I

L

T

A

G

G

T

D

G

G

I

G

G

I
x
G

G

G

A

I

G

I

M

H

F

-

L

-

D

Q

G

H

H

E

L

L

Y

I

K

H

G

G

S

S

R

S

A

F

N

C

A

A

G
x
A

E

E

I

L

G

G

H
|
H

I

L

I

V

V

V

E

S

P

L

H

N

G

G

R

P

L

D

C
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C

T

S

C
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C

G

G

K

S

R

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G

G

C
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C

L

I

D

E

R

A

Y

Y

V

A

S

S

P

-

S

G

V

M

A

A

Y

L

E

Q

C

-

L

-

G

-

I

-

E

R

D

E

A

A

Q

K

E

K

L

L

D

H

P

D

D

E

D

D

L

L

binding calcium ion: N112 (= N192), N115 (≠ T195), G144 (≠ I224), A161 (≠ G243)
binding zinc ion: H165 (= H247), C175 (= C257), C177 (= C259), C182 (= C264)

3vglA Crystal structure of a rok family glucokinase from streptomyces griseus in complex with glucose and amppnp (see paper)
38% identity, 30% coverage: 147:270/408 of query aligns to 59:179/312 of 3vglA

query
sites
3vglA

V

V

G

G

I

I

A

G

L

A

P

A

G
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G

R

Y

Y

V

A

D

D

D

G

K

G

R

V

A

T

T

S

V

L

L

S

F

P

A

L
x
P

N

N

L

I

P

D

G

-

W

W

Q

R

N

H

F

E

P

P

V

L

A

K

R

D

E

K

L

V

E

E

R

Q

L

R

I

V

G

G

L

L

P

P

V

V

L

V

V

V

E

E

N
|
N

D
|
D

A

A

T

N

A

A

G

A

A

A

I

W

G

G

E

E

R

Y

L

R

Y

F

G

G

V

A

A

G

R

Q

G

G

L

H

G

D

S

D

F

V

V

I

Y

C

L

I

F

T

L

L

A
x
G

G
x
T

D

G

G

-

I
x
L

G
|
G

A

G

G

G

M

I

F

I

L

I

D

G

G

N

H

K

L

L

Y

R

K

R

G

G

S

R

R

F

A

G

N

V

A

A

G

A

E
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E

I

F

G

G

H
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H

I

I

I

R

V

V

E

V

P

P

H

D

G

G

R

L

L

L

C
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C

T

G

C
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C

G

G

K

S

R

Q

G

G

C
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C

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W

D
x
E

R

Q

Y

Y

V

A

S

S

binding phosphoaminophosphonic acid-adenylate ester: G130 (≠ A219), T131 (≠ G220)
binding beta-D-glucopyranose: G65 (= G153), P78 (≠ L166), N103 (= N192), D104 (= D193), L133 (≠ I224), G134 (= G225), E153 (= E244), H156 (= H247), E175 (≠ D266)
binding zinc ion: H156 (= H247), C166 (= C257), C168 (= C259), C173 (= C264)

Sites not aligning to the query:

binding phosphoaminophosphonic acid-adenylate ester: 9, 11, 12, 180, 214, 218, 260, 261, 264

Query Sequence

>WP_004110897.1 NCBI__GCF_000359745.1:WP_004110897.1
MKTISGTNLEQAKSHNRRVVIEAIRTNGPLSRAAIARLTALTTQTVSNIVEELARSHLLI
PMEAQKIARGQPIIPYTINPSGAYSIGLELGRQRAAGVLTDLSGTVCARIDRHVDRPGPT
EAMPVFAAIVRDLQEAFSFDRDRLLGVGIALPGRYADGGVTSLSPLNLPGWQNFPVAREL
ERLIGLPVLVENDATAGAIGERLYGVARGLGSFVYLFLAGDGGIGAGMFLDGHLYKGSRA
NAGEIGHIIVEPHGRLCTCGKRGCLDRYVSPSVAYECLGIEDAQELDPDDLDAMIAANSR
GLQTWLEQAVQPLRQTIDFLELAFDPQTIVLGGSAPPSLMTGLAERVEPLHIPVNPMEER
KIPRLMVGATGKDTAILGAAALPIFSETNPQFDVLQKPIIHRAAEQAG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory