SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_004117390.1 NCBI__GCF_000359745.1:WP_004117390.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

1vpeA Crystallographic analysis of phosphoglycerate kinase from the hyperthermophilic bacterium thermotoga maritima (see paper)
48% identity, 97% coverage: 12:400/400 of query aligns to 9:397/398 of 1vpeA

query
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1vpeA

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active site: R35 (= R38), K196 (= K199), G353 (= G356), G376 (= G379)
binding phosphoaminophosphonic acid-adenylate ester: G194 (= G197), A195 (= A198), K196 (= K199), K200 (= K203), G218 (= G221), A219 (≠ G222), N316 (= N318), P318 (= P320), G320 (= G322), V321 (≠ A323), E323 (= E325), G352 (= G355), G353 (= G356), D354 (= D357), S355 (≠ T358)

P36204 Bifunctional PGK/TIM; EC 2.7.2.3; EC 5.3.1.1 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see paper)
48% identity, 97% coverage: 12:400/400 of query aligns to 10:398/654 of P36204

query
sites
P36204

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R36 (= R38) binding substrate
R118 (= R120) binding substrate
R151 (= R153) binding substrate

1phpA Structure of the adp complex of the 3-phosphoglycerate kinase from bacillus stearothermophilus at 1.65 angstroms (see paper)
50% identity, 98% coverage: 5:397/400 of query aligns to 4:392/394 of 1phpA

query
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1phpA

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active site: R36 (= R38), K197 (= K199), G351 (= G356), G374 (= G379)
binding adenosine-5'-diphosphate: G195 (= G197), K201 (= K203), G219 (= G221), G220 (= G222), L237 (= L239), N316 (= N318), P318 (= P320), G320 (= G322), V321 (≠ A323), E323 (= E325), G350 (= G355), D352 (= D357), S353 (≠ T358)

P18912 Phosphoglycerate kinase; EC 2.7.2.3 from Geobacillus stearothermophilus (Bacillus stearothermophilus) (see paper)
50% identity, 98% coverage: 5:397/400 of query aligns to 4:392/394 of P18912

query
sites
P18912

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G

G

A

A

K

K

V

V

I

I

L

L

L

A

A

S

H

H

F

L

G

G

R

R

P

P

K

K

D

G

G

K

P

V

S

V

P

E

D

E

L

L

S

R

L

L

G

D

L

A

I

V

V

A

P

K

A

R

V

L

E

G

E

E

V

L

L

L

D

E

H

R

A

P

V

V

L

A

F

K

A

T

S

N

D

E

C

A

I

V

G

G

A

D

A

E

A

V

A

K

D

A

A

A

V

V

A

D

K

R

M

L

N

N

D

E

G

G

D

D

I

V

L

L

L

L

L

L

E

E

N

N

T

V

R

R

F

F

H

Y

K

P

G

G

E

E

E

E

K

K

N

N

D

D

A

P

A

E

F

L

T

A

K

K

A

A

L

F

A

A

A

E

N

L

G

A

D

D

L

L

Y

Y

V

V

N

N

D

D

A

A

F

F

S

G

A

A

A

A

H

H

R

R

A

A

H

H

C

A

S

S

T

T

E

E

G

G

L

I

A

A

Q

H

L

Y

L

L

P

P

A

A

Y

V

A

A

G

G

R

F

T

L

M

M

Q

E

A

K

E

E

L

L

E

E

A

V

L

L

E

G

K

K

G

A

L

L

G

S

N

N

P

P

V

D

R

R

P

P

V

F

V

T

A

A

I

I

V

I

G

G

G

G

A

A

K

K

V

V

S

K

T

D

K
|
K

I

I

D

G

L

V

L

I

M

D

N

N

L

L

V

L

K

E

K

K

V

V

D

D

A

N

L

L

V

I

I

I

G

G

G

G

G

G

M

L

A

A

N

Y

T

T

F

F

L

V

A

K

A

A

R

L

G

G

T

H

N

D

V

V

G

G

K

K

S

S

L

L

C

L

E

E

H

E

D

D

L

K

A

I

D

E

T

L

A

A

K

K

Q

S

I

F

M

M

I

E

E

K

A

A

A

K

A

E

S

K

G

G

C

V

A

R

I

F

V

Y

L

M

P

P

E

V

D

D

G

V

V

V

I

V

A

A

R

D

E

R

F

F

K

A

A

N

G

D

A

A

D

N

N

T

E

K

V

V

V

V

A

P

I

I

E

D

A

A

I

I

P

P

A

A

D

D

A

W

M

S

V

A

L

L

D

D

V

I

G

G

P

P

K

K

S

T

V

R

E

E

A

L

I

Y

K

R

N

D

W

V

I

I

G

R

R

E

A

S

T

K

T

L

L

V

V

V

W

W

N
|
N

G

G

P

P

L

M

G

G

A

V

F

F

E
|
E

I

M

T

D

P

A

F

F

D

A

A

H

A

G

T

T

V

K

A

A

A

I

A

A

K

E

Y

A

A

L

A

A

E

E

C

A

T

L

K

D

A

T

G

-

K

-

L

-

T

Y

S

S

V

V

A

I

G

G

G
|
G

G
|
G

D
|
D

T
x
S

V

A

S

A

A

A

L

V

N

E

H

K

A

F

G

G

V

L

A

A

D

D

D

K

F

M

T

D

Y

H

V

I

S

S

T

T

A

G

G

G

G

G

A

A

F

S

L

L

E

E

W

F

M

M

E

E

G

G

K

K

V

Q

L

L

P

P

G

G

V

V

A

V

V

A

L

L

Q

E

K201 (= K203) binding ATP
N316 (= N318) binding ATP
E323 (= E325) binding ATP
GGDS 350:353 (≠ GGDT 355:358) binding ATP

P40924 Phosphoglycerate kinase; EC 2.7.2.3 from Bacillus subtilis (strain 168) (see paper)
48% identity, 98% coverage: 5:397/400 of query aligns to 4:392/394 of P40924

query
sites
P40924

K

K

T

T

L

L

D

K

N

D

L

I

T

-

D

D

V

V

T

K

G

G

K

K

R

V

V

V

L

F

V

C

R

R

V

V

D

D

L

F

N

N

V

V

P

P

V

M

K

K

D

D

G

G

K

E

V

V

T

T

D

D

A

D

T

T

R

R

I

I

E

R

R

A

V

A

A

L

P

P

T

T

I

I

L

K

E

H

L

L

S

A

E

D

K

Q

G

G

A

A

K

K

V

V

I

L

L

L

L

A

A

S

H

H

F

L

G

G

R

R

P

P

K

K

D

G

G

E

P

V

S

V

P

E

D

E

L

L

S

R

L

L

G

T

L

P

I

V

V

A

P

A

A

R

V

L

E

G

E

E

V

L

L

L

D

G

H

K

A

E

V

V

L

K

F

K

A

A

S

D

D

E

C

A

I

Y

G

G

A

D

A

A

A

V

A

K

D

A

A

Q

V

I

A

S

K

E

M

M

N

K

D

D

G

G

D

D

I

V

L

L

L

V

L

L

E

E

N

N

T

V

R

R

F

F

H

Y

K

P

G

G

E

E

E

E

K

K

N

N

D

D

A

P

A

E

F

L

T

A

K

K

A

A

L

F

A

A

A

E

N

L

G

A

D

D

L

V

Y

Y

V

V

N

N

D

D

A

A

F

F

S

G

A

A

A

A

H

H

R

R

A

A

H

H

C

A

S

S

T

T

E

A

G

G

L

I

A

A

Q

E

L

H

L

L

P

P

A

A

Y

V

A

A

G

G

R

F

T

L

M

M

Q

E

A

K

E

E

L

L

E

D

A

V

L

L

E

G

K

K

G

A

L

V

G
x
S

N

N

P

P

V

D

R

R

P

P

V

F

V

T

A

A

I

I

V

I

G

G

G

G

A

A

K

K

V

V

S

K

T

D

K

K

I

I

D

G

L

V

L

I

M

E

N

S

L

L

V

L

K

D

K

K

V

V

D

D

A

N

L

L

V

I

I

I

G

G

G

G

G

G

M

L

A

A

N

Y

T

T

F

F

L

V

A

K

A

A

R

L

G

G

T

Y

N

E

V

V

G

G

K

K

S

S

L

L

C

L

E

E

H

E

D

D

L

K

A

I

D

E

T

L

A

A

K

K

Q

S

I

F

M

M

I

D

E

R

A

A

A

K

A

E

S

K

G

G

C

V

A

N

I

F

V

Y

L

M

P

P

E

E

D

D

G

V

V

L

I

V

A

A

R

D

E

D

F

F

K

S

A

N

G

D

A

A

D

N

N

V

E

K

V

I

V

V

A

P

I

I

E

S

A

E

I

I

P

P

A

S

D

D

A

L

M

E

V

A

L

I

D

D

V

I

G

G

P

T

K

K

S
x
T

V

R

E

E

A

T

I

Y

K

A

N

D

W

V

I

I

G

K

R

N

A

S

T

K

T

L

L

V

V

V

W

W

N

N

G

G

P

P

L

M

G

G

A

V

F

F

E

E

I

I

T

D

P

L

F

F

D

A

A

Q

A

G

T

T

V

K

A

A

A

V

A

A

K

E

Y

A

A

L

A

A

E

E

C

-

T

-

K

-

A

A

G

K

K

D

L

T

T

Y

S

S

V

V

A

I

G

G

G

G

G

G

D

D

T

S

V

A

S

A

A

A

L

V

N

E

H

K

A

F

G

G

V

L

A

A

D

D

D

K

F

M

T

S

Y

H

V

I

S

S

T

T

A

G

G

G

G

G

A

A

F

S

L

L

E

E

W

F

M

M

E

E

G

G

K

K

V

E

L

L

P

P

G

G

V

V

A

A

V

A

L

L

Q

N

S183 (≠ G185) modified: Phosphoserine
T299 (≠ S301) modified: Phosphothreonine

4feyA An x-ray structure of a putative phosphogylcerate kinase with bound adp from francisella tularensis subsp. Tularensis schu s4
44% identity, 99% coverage: 3:397/400 of query aligns to 2:387/392 of 4feyA

query
sites
4feyA

A

S

F

F

K

L

T

T

L

L

D

K

N

D

L

V

T

-

D

D

V

L

T

K

G

D

K

K

R

K

V

V

L

L

V

V

R

R

V

V

D

D

L

F

N

N

V

V

P

P

V

V

K

K

D

D

G

G

K

K

V

V

T

T

D

S

A

K

T

V

R
|
R

I

I

E

E

R

A

V

A

A

I

P

P

T

T

I

I

L

Q

E

Y

L

I

S

L

E

D

K

Q

G

G

A

G

K

A

V

V

I

I

L

L

L

M

A

S

H

H

F

L

G

G

R

R

P

P

K

T

D

E

G

G

P

E

-

Y

S

D

P

S

D

Q

L

F

S

S

L

L

G

E

L

P

I

V

V

A

P

K

A

A

V

L

E

S

E

E

V

I

L

I

D

N

H

K

A

P

V

V

L

K

F

F

A

A

S

K

D

D

C

W

I

L

G

D

A

G

A

V

A

D

A

V

D

K

A

A

V

-

A

-

K

-

M

-

N

-

D

-

G

G

D

E

I

I

L

V

L

M

L

C

E

E

N

N

T

V

R

R

F

F

H

N

K

S

G

G

E

E

E

K

K

K

N

S

D

T

A

D

A

D

F

L

T

S

K

K

A

K

L

I

A

A

A

S

N

L

G

G

D

D

L

V

Y

F

V

V

N

M

D

D

A

A

F

F

S

A

A

T

A

A

H

H

R

R

A

A

H

Q

C

A

S

S

T

T

E

Y

G

G

L

V

A

A

Q

K

L

Y

L

I

P

P

-

V

A

A

Y

C

A

A

G

G

R

I

T

L

M

L

Q

T

A

N

E

E

L

I

E

Q

A

A

L

L

E

E

K

K

G

A

L

L

G

K

N

S

P

P

V

K

R

K

P

P

V

M

V

A

A

A

I

I

V

V

G

G

G
|
G

A
x
S

K
|
K

V

V

S

S

T

T

K
|
K

I

L

D

S

L

V

L

L

M

N

N

N

L

L

V

L

K

D

K

K

V

V

D

E

A

I

L

L

V

I

I

V

G

G

G
|
G

G

G

M

I

A

A

N

N

T

T

F

F

L

I

A

K

A

A

R

E

G

G

T

F

N

D

V

V

G

G

K

N

S

S

L

L

C

Y

E

E

H

Q

D

D

L

L

A

V

D

A

T

E

A

A

K

T

Q

E

I

I

M

L

I

A

E

K

A

A

A

K

A

A

S

L

G

G

C

V

A

N

I

I

V

P

L

V

P

P

E

V

D

D

G

V

V

R

I

V

A

A

R

K

E

E

F

F

K

S

A

E

G

N

A

A

D

Q

N

A

E

I

V

I

V

K

A

K

I

V

E

S

A

D

I

V

P

V

A

A

D

D

A

E

M

M

V

I

L

L

D

D

V

I

G

G

P

P

K

E

S

S

V

Q

E

K

A

I

I

I

K

A

N

E

W

L

I

L

G

K

R

S

A

A

T

N

T

T

L

I

V

L

W

W

N

N

G

G

P

P

L

V

G
|
G

A
x
V

F

F

E
|
E

I

F

T

D

P

N

F

F

D

A

A

E

A

G

T

T

V

K

A

A

A

L

A

S

K

L

Y

A

A

I

A

A

E

Q

C

S

T

-

K

-

A

-

G

-

K

H

L

A

T

F

S

S

V

V

A

A

G

G

G

G

G
|
G

D
|
D

T

T

V

I

S

A

A

A

L

I

N

E

H

K

A

F

G

G

V

I

A

K

D

D

D

Q

F

V

T

S

Y

Y

V

I

S

S

T

T

A

A

G

G

G
|
G

A

A

F

F

L

L

E

E

W

F

M

L

E

E

G

G

K

K

V

K

L

L

P

P

G

A

V

I

A

E

V

I

L

L

Q

K

active site: R36 (= R38), K193 (= K199), G346 (= G356), G369 (= G379)
binding adenosine-5'-diphosphate: G191 (= G197), S192 (≠ A198), K197 (= K203), G215 (= G221), G316 (= G322), V317 (≠ A323), E319 (= E325), D347 (= D357)

P07378 Phosphoglycerate kinase, glycosomal; Phosphoglycerate kinase C; EC 2.7.2.3 from Trypanosoma brucei brucei (see 2 papers)
43% identity, 97% coverage: 9:396/400 of query aligns to 10:416/440 of P07378

query
sites
P07378

N

N

L

E

T

C

D

D

V

L

T

K

G

G

K

K

R

K

V

V

L

L

V

I

R

R

V

V

D
|
D

L
x
F

N
|
N

V

V

P

P

V

V

K

K

D

N

G

G

K

K

V

I

T

T

D

N

A

D

T

Y

R
|
R

I

I

E

R

R

S

V

A

A

L

P

P

T

T

I

L

L

K

E

K

L

V

S

L

E

T

K

E

G

G

A

G

K

S

V

C

I

V

L

L

L

M

A

S

H
|
H

F
x
L

G
|
G

R
|
R

P

P

K

K

D

G

G

I

P

P

-

M

-

A

-

Q

-

A

-

G

-

K

-

I

-

R

-

S

-

T

-

G

-

G

-

V

-

P

-

G

-

F

S

Q

P

Q

D

K

L

A

S

T

L

L

G

K

L

P

I

V

V

A

P

K

A

A

V

L

E

S

E

E

V

L

L

L

D

L

H

R

A

P

V

V

L

T

F

F

A

A

S

P

D

D

C

C

I

L

G

N

A

A

A

A

A

-

A

-

D

D

A

V

V

V

A

S

K

K

M

M

N

S

D

P

G

G

D

D

I

V

L

V

L

L

L

L

E

E

N

N

T

V

R
|
R

F

F

H

Y

K

K

G

E

E

E

-

G

-

S

-

K

-

K

E

A

K

K

N

D

D

R

A

E

A

A

F

M

T

A

K

K

A

I

L

L

A

A

A

S

N

Y

G

G

D

D

L

V

Y

Y

V

I

N

S

D

D

A

A

F

F

S

G

A

T

A

A

H

H

R
|
R

A

D

H

S

C

A

S

T

T

M

E

T

G

G

L

I

A

P

Q

K

L

I

L

L

-

G

P

N

A

G

Y

A

A

A

G

G

R

Y

T

L

M

M

Q

E

A

K

E

E

L

I

E

S

A

Y

L

F

E

A

K

K

G

V

L

L

G

G

N

N

P

P

V

P

R

R

P

P

V

L

V

V

A

A

I

I

V

V

G

G

G

G

A

A

K

K

V

V

S

S

T

D

K
|
K

I

I

D

Q

L

L

L

L

M

D

N

N

L

M

V

L

K

Q

K

R

V

I

D

D

A

Y

L

L

V

L

I

I

G

G

G

G

G

A

M

M

A

A

N

Y

T

T

F

F

L

L

A

K

A

A

R

Q

G

G

T

Y

N

S

V

I

G

G

K

K

S

S

L

K

C

C

E

E

H

E

D

S

L

K

A

L

D

E

T

F

A

A

K

R

Q

S

I

L

M

L

I

K

E

K

A

A

A

E

A

D

S

R

G

K

C

V

A

Q

I

V

V

I

L

L

P

P

E

I

D

D

G

H

V

V

I

C

A

H

R

T

E

E

F

F

K

K

A

A

G

V

A

D

D

S

N

P

E

L

V

I

V

T

A

E

I

D

E

Q

A

N

I

I

P

P

A

E

D

G

A

H

M

M

V

A

L

L

D

D

V

I

G

G

P

P

K

K

S

T

V

I

E

E

A

K

I

Y

K

V

N

Q

W

T

I

I

G

G

R

K

A

C

T

K

T

S

L

A

V

I

W

W

N
|
N

G

G

P

P

L

M

G

G

A

V

F

F

E
|
E

I

M

T

V

P

P

F

Y

D

S

A

K

A

G

T

T

V

F

A

A

A

I

A

A

K

K

Y

A

A

M

A

G

E

R

C

G

T

T

K

H

A

E

G

H

K

G

L

L

T

M

S

S

V

I

A

I

G

G

G
|
G

G
|
G

D
|
D

T
x
S

V

A

S

S

A

A

L

A

N

E

H

L

A

S

G

G

V

E

A

A

D

K

D

R

F

M

T

S

Y

H

V

V

S

S

T

T

A

G

G

G

G

G

A

A

F

S

L

L

E

E

W

L

M

L

E

E

G

G

K

K

V

T

L

L

P

P

G

G

V

V

A

T

V

V

L

L

DFN 24:26 (≠ DLN 23:25) binding substrate
R39 (= R38) binding substrate
HLGR 62:65 (≠ HFGR 61:64) binding substrate
R135 (= R120) binding substrate
R172 (= R153) binding substrate
K223 (= K203) binding ATP
N338 (= N318) binding ATP
E345 (= E325) binding ATP
GGDS 375:378 (≠ GGDT 355:358) binding ATP

16pkA Phosphoglycerate kinase from trypanosoma brucei bisubstrate analog (see paper)
42% identity, 97% coverage: 9:396/400 of query aligns to 6:412/415 of 16pkA

query
sites
16pkA

N

N

L

E

T

C

D

D

V

L

T

K

G

G

K

K

R

K

V

V

L

L

V

I

R

R

V

V

D

D

L

F

N

N

V

V

P

P

V

V

K

K

D

N

G

G

K

K

V

I

T

T

D

N

A

D

T

Y

R
|
R

I

I

E

R

R

S

V

A

A

L

P

P

T

T

I

L

L

K

E

K

L

V

S

L

E

T

K

E

G

G

A

G

K

S

V

C

I

V

L

L

L

M

A

S

H

H

F

L

G

G

R

R

P

P

K

K

D

G

G

I

P

P

-

M

-

A

-

Q

-

A

-

G

-

K

-

I

-

R

-

S

-

T

-

G

-

G

-

V

-

P

-

G

-

F

S

Q

P

Q

D

K

L

A

S

T

L

L

G

K

L

P

I

V

V

A

P

K

A

R

V

L

E

S

E

E

V

L

L

L

D

L

H

R

A

P

V

V

L

T

F

F

A

A

S

P

D

D

C

C

I

L

G

N

A

A

A

A

A

-

A

-

D

D

A

V

V

V

A

S

K

K

M

M

N

S

D

P

G

G

D

D

I

V

L

V

L

L

L

L

E

E

N

N

T

V

R

R

F

F

H

Y

K

K

G

E

E

E

-

G

-

S

-

K

-

K

E

A

K

K

N

D

D

R

A

E

A

A

F

M

T

A

K

K

A

I

L

L

A

A

A

S

N

Y

G

G

D

D

L

V

Y

Y

V

I

N

S

D

D

A

A

F

F

S

G

A

T

A

A

H

H

R

R

A

D

H

S

C

A

S

T

T

M

E

T

G

G

L

I

A

P

Q

K

L

I

L

L

-

G

P

N

A

G

Y

A

A

A

G

G

R

Y

T

L

M

M

Q

E

A

K

E

E

L

I

E

S

A

Y

L

F

E

A

K

K

G

V

L

L

G

G

N

N

P

P

V

P

R

R

P

P

V

L

V

V

A

A

I

I

V

V

G

G

G
|
G

A
|
A

K
|
K

V

V

S

S

T

D

K
|
K

I

I

D

Q

L

L

L

L

M

D

N

N

L

M

V

L

K

Q

K

R

V

I

D

D

A

Y

L

L

V

L

I

I

G

G

G

G

G
x
A

M

M

A

A

N
x
Y

T

T

F

F

L

L

A

K

A

A

R

Q

G

G

T

Y

N

S

V

I

G

G

K

K

S

S

L

K

C

C

E

E

H

E

D

S

L

K

A

L

D

E

T

F

A

A

K

R

Q

S

I

L

M

L

I

K

E

K

A

A

A

E

A

D

S

R

G

K

C

V

A

Q

I

V

V

I

L

L

P

P

E

I

D

D

G

H

V

V

I

C

A

H

R

T

E

E

F

F

K

K

A

A

G

V

A

D

D

S

N

P

E

L

V

I

V

T

A

E

I

D

E

Q

A

N

I

I

P

P

A

E

D

G

A

H

M

M

V

A

L
|
L

D

D

V

I

G

G

P

P

K

K

S

T

V

I

E

E

A

K

I

Y

K

V

N

Q

W

T

I

I

G

G

R

K

A

C

T

K

T

S

L

A

V

I

W

W

N

N

G

G

P
|
P

L

M

G
|
G

A
x
V

F

F

E
|
E

I

M

T

V

P

P

F

Y

D

S

A

K

A

G

T

T

V

F

A

A

A

I

A

A

K

K

Y

A

A

M

A

G

E

R

C

G

T

T

K

H

A

E

G

H

K

G

L

L

T

M

S

S

V

I

A

I

G

G

G

G

G
|
G

D

D

T

S

V

A

S

S

A

A

L

A

N

E

H

L

A

S

G

G

V

E

A

A

D

K

D

R

F

M

T

S

Y

H

V

V

S

S

T

T

A
x
G

G
|
G

G
|
G

A

A

F

S

L

L

E

E

W

L

M

L

E

E

G

G

K

K

V

T

L

L

P

P

G

G

V

V

A

T

V

V

L

L

active site: R35 (= R38), K215 (= K199), G372 (= G356), G395 (= G379)
binding 1,1,5,5-tetrafluorophosphopentylphosphonic acid adenylate ester: G213 (= G197), A214 (= A198), K219 (= K203), A238 (≠ G222), Y241 (≠ N225), L311 (= L295), P336 (= P320), G338 (= G322), V339 (≠ A323), E341 (= E325), G393 (≠ A377), G394 (= G378), G395 (= G379)

13pkA Ternary complex of phosphoglycerate kinase from trypanosoma brucei (see paper)
42% identity, 97% coverage: 9:396/400 of query aligns to 6:412/415 of 13pkA

query
sites
13pkA

N

N

L

E

T

C

D

D

V

L

T

K

G

G

K

K

R

K

V

V

L

L

V

I

R

R

V

V

D

D

L

F

N

N

V

V

P

P

V

V

K

K

D

N

G

G

K

K

V

I

T

T

D

N

A

D

T

Y

R
|
R

I

I

E

R

R

S

V

A

A

L

P

P

T

T

I

L

L

K

E

K

L

V

S

L

E

T

K

E

G

G

A

G

K

S

V

C

I

V

L

L

L

M

A

S

H

H

F

L

G

G

R

R

P

P

K

K

D

G

G

I

P

P

-

M

-

A

-

Q

-

A

-

G

-

K

-

I

-

R

-

S

-

T

-

G

-

G

-

V

-

P

-

G

-

F

S

Q

P

Q

D

K

L

A

S

T

L

L

G

K

L

P

I

V

V

A

P

K

A

R

V

L

E

S

E

E

V

L

L

L

D

L

H

R

A

P

V

V

L

T

F

F

A

A

S

P

D

D

C

C

I

L

G

N

A

A

A

A

A

-

A

-

D

D

A

V

V

V

A

S

K

K

M

M

N

S

D

P

G

G

D

D

I

V

L

V

L

L

L

L

E

E

N

N

T

V

R

R

F

F

H

Y

K

K

G

E

E

E

-

G

-

S

-

K

-

K

E

A

K

K

N

D

D

R

A

E

A

A

F

M

T

A

K

K

A

I

L

L

A

A

A

S

N

Y

G

G

D

D

L

V

Y

Y

V

I

N

S

D

D

A

A

F

F

S

G

A

T

A

A

H

H

R

R

A

D

H

S

C

A

S

T

T

M

E

T

G

G

L

I

A

P

Q

K

L

I

L

L

-

G

P

N

A

G

Y

A

A

A

G

G

R

Y

T

L

M

M

Q

E

A

K

E

E

L

I

E

S

A

Y

L

F

E

A

K

K

G

V

L

L

G

G

N

N

P

P

V

P

R

R

P

P

V

L

V

V

A

A

I

I

V

V

G

G

G
|
G

A
|
A

K
|
K

V

V

S

S

T

D

K
|
K

I

I

D

Q

L

L

L

L

M

D

N

N

L

M

V

L

K

Q

K

R

V

I

D

D

A

Y

L

L

V

L

I

I

G

G

G

G

G

A

M

M

A

A

N

Y

T

T

F

F

L

L

A

K

A

A

R

Q

G

G

T

Y

N

S

V

I

G

G

K

K

S

S

L

K

C

C

E

E

H

E

D

S

L

K

A

L

D

E

T

F

A

A

K

R

Q

S

I

L

M

L

I

K

E

K

A

A

A

E

A

D

S

R

G

K

C

V

A

Q

I

V

V

I

L

L

P

P

E

I

D

D

G

H

V

V

I

C

A

H

R

T

E

E

F

F

K

K

A

A

G

V

A

D

D

S

N

P

E

L

V

I

V

T

A

E

I

D

E

Q

A

N

I

I

P

P

A

E

D

G

A

H

M

M

V

A

L
|
L

D

D

V

I

G

G

P

P

K

K

S

T

V

I

E

E

A

K

I

Y

K

V

N

Q

W

T

I

I

G

G

R

K

A

C

T

K

T

S

L

A

V

I

W

W

N

N

G

G

P
|
P

L

M

G
|
G

A
x
V

F

F

E
|
E

I

M

T

V

P

P

F

Y

D

S

A

K

A

G

T

T

V

F

A

A

A

I

A

A

K

K

Y

A

A

M

A

G

E

R

C

G

T

T

K

H

A

E

G

H

K

G

L

L

T

M

S

S

V

I

A

I

G

G

G
|
G

G
|
G

D
|
D

T
x
S

V

A

S

S

A

A

L

A

N

E

H

L

A

S

G

G

V

E

A

A

D

K

D

R

F

M

T

S

Y

H

V

V

S

S

T

T

A

G

G

G

G
|
G

A

A

F

S

L

L

E

E

W

L

M

L

E

E

G

G

K

K

V

T

L

L

P

P

G

G

V

V

A

T

V

V

L

L

active site: R35 (= R38), K215 (= K199), G372 (= G356), G395 (= G379)
binding adenosine-5'-diphosphate: G213 (= G197), A214 (= A198), K219 (= K203), L311 (= L295), P336 (= P320), G338 (= G322), V339 (≠ A323), E341 (= E325), G371 (= G355), D373 (= D357), S374 (≠ T358)

4ng4B Structure of phosphoglycerate kinase (cbu_1782) from coxiella burnetii (see paper)
43% identity, 98% coverage: 4:396/400 of query aligns to 2:384/389 of 4ng4B

query
sites
4ng4B

F

F

K

L

T

S

L

M

D

S

N

N

L

L

T

-

D

N

V

L

T

H

G

N

K

K

R

R

V

V

L

M

V

I

R

R

V

E

D

D

L

L

N

N

V

V

P

P

V

M

K

K

D

N

G

G

K

K

V

I

T

T

D

N

A

D

T

E

R
|
R

I

I

E

V

R

R

V

A

A

L

P

P

T

T

I

I

L

Q

E

K

L

A

S

I

E

E

K

Q

G

K

A

A

K

R

V

V

I

M

L

I

L

L

A

S

H

H

F

L

G

G

R

R

P

P

K

E

D

E

G

G

P

K

-

F

S

E

P

K

D

E

L

F

S

S

L

L

G

A

L

P

I

V

V

A

P

R

A

L

V

L

E

S

E

K

V

K

L

L

D

N

H

K

A

V

V

P

L

L

F

I

A

-

S

N

D

D

C

W

I

L

G

K

A

G

A

V

A

A

A

V

D

E

A

P

V

-

A

-

K

-

M

-

N

-

D

-

G

G

D

Q

I

A

L

I

L

L

L

C

E

E

N

N

T

V

R

R

F

F

H

N

K

K

G

G

E

E

E

N

K

E

N

N

D

N

A

T

A

E

F

L

T

A

K

K

A

R

L

M

A

A

A

E

N

L

G

C

D

D

L

I

Y

F

V

V

N

M

D

D

A

A

F

F

S

A

A

T

A

A

H

H

R

R

A

A

H

Q

C

A

S

S

T

T

E

A

G

G

L

V

A

A

Q

A

L

Y

L

A

P

K

-

L

A

A

Y

C

A

A

G

G

R

P

T

L

M

L

Q

I

A

S

E

E

L

V

E

E

A

A

L

L

E

S

K

R

G

A

L

L

G

E

N

N

P

P

V

Q

R

K

P

P

V

L

V

V

A

A

I

V

V

V

G

G

G
|
G

A

S

K
|
K

V

V

S

S

T

T

K
|
K

I

I

D

H

L

L

L

L

M

E

N

N

L

L

V

L

K

D

K

K

V

V

D

D

A

Q

L

L

V

I

I

V

G

G

G
|
G

G

G

M

I

A

A

N

N

T

T

F

F

L

L

A

K

A

A

R

Q

G

G

T

Y

N

S

V

I

G

G

K

K

S

S

L

L

C

C

E

E

H

N

D

E

L

W

A

L

D

D

T

A

A

A

K

Q

Q

Q

I

F

M

W

I

E

E

K

A

A

A

A

A

E

S

K

G

N

C

V

A

S

I

L

V

P

L

L

P

P

E

V

D

D

G

V

V

I

I

V

A

A

R

D

E

E

F

L

K

S

A

E

G

D

A

A

D

K

N

A

E

T

V

V

V

K

A

N

I

I

E

D

A

A

I

V

P

T

A

S

D

N

A

E

M

S

V
x
I

L

F

D
|
D

V

V

G

G

P

P

K

N

S

T

V

S

E

A

A

T

I

Y

K

A

N

K

W

L

I

M

G

A

R

Q

A

A

T

G

T

T

L

I

V

V

W

W

N
|
N

G
|
G

P
|
P

L

I

G
|
G

A
x
V

F

F

E
|
E

I

I

T

E

P

A

F
|
F

D
x
S

A

Q

A

G

T
|
T

V

R

A

A

A

L

A

A

K

Q

Y

A

A

V

A

A

E

K

C

S

T

T

K

A

A

-

G

-

K

-

L

-

T

Y

S

S

V

I

A

V

G

G

G
|
G

G
|
G

D
|
D

T
|
T

V

L

S

A

A

A

L

L

N

D

H

K

A

F

G

N

V

L

A

T

D

D

D

Q

F

M

T

S

Y

Y

V

V

S

S

T

T

A

A

G

G

G
|
G

A

A

F

F

L

L

E

E

W

F

M

L

E

E

G

G

K

K

V

I

L

L

P

P

G

A

V

I

A

K

V

I

L

L

active site: R35 (= R38), K191 (= K199), G344 (= G356), G367 (= G379)
binding adenosine-5'-diphosphate: G189 (= G197), K195 (= K203), G213 (= G221), I286 (≠ V294), N310 (= N318), G311 (= G319), P312 (= P320), V315 (≠ A323), E317 (= E325), G343 (= G355), D345 (= D357), T346 (= T358)
binding magnesium ion: D288 (= D296), G314 (= G322), F321 (= F329), S322 (≠ D330), T325 (= T333)

P0A799 Phosphoglycerate kinase; EC 2.7.2.3 from Escherichia coli (strain K12) (see 3 papers)
44% identity, 99% coverage: 1:397/400 of query aligns to 1:382/387 of P0A799

query
sites
P0A799

M
|
M

A

S

A

V

F

I

K

K

T

M

L

T

D

D

N

-

L

-

T

L

D

D

V

L

T

A

G

G

K

K

R

R

V

V

L

F

V

I

R

R

V

A

D

D

L

L

N

N

V

V

P

P

V

V

K

K

D

D

G

G

K

K

V

V

T

T

D

S

A

D

T

A

R

R

I

I

E

R

R

A

V

S

A

L

P

P

T

T

I

I

L

E

E

L

L

A

S

L

E

K

K

Q

G

G

A

A

K

K

V

V

I

M

L

V

L

T

A

S

H

H

F

L

G

G

R

R

P

P

K

T

D

E

G

G

P

E

-

Y

S

N

P

E

D

E

L

F

S

S

L

L

G

L

L

P

I

V

V

V

P

N

A

Y

V

L

E

K

E

D

V
x
K

L

L

D

S

H

N

A

P

V

V

L

R

F

L

A

V

S

K

D

D

C

Y

I

L

G

-

A

-

A

-

A

-

A

-

D

D

A

G

V

V

A

-

K

D

M

V

N

A

D

E

G

G

D

E

I

L

L

V

L

V

L

L

E

E

N

N

T

V

R

R

F

F

H

N

K

K

G

G

E

E

E

K

K

K

N

D

D

D

A

E

A

T

F

L

T

S

K

K

A

K

L

Y

A

A

A

A

N

L

G

C

D

D

L

V

Y

F

V

V

N

M

D

D

A

A

F

F

S

G

A

T

A

A

H

H

R

R

A

A

H

Q

C

A

S

S

T

T

E

H

G

G

L

I

A

G

Q

K

L

F

L

A

P

D

-

V

A

A

Y

C

A

A

G

G

R

P

T

L

M

L

Q

A

A

A

E

E

L

L

E

D

A

A

L

L

E

G

K

K

G

A

L

L

G

K

N

E

P

P

V

A

R

R

P

P

V

M

V

V

A

A

I

I

V

V

G

G

G

G

A

S

K

K

V

V

S

S

T

T

K

K

I

L

D

T

L

V

L

L

M

D

N

S

L

L

V

S

K

K

K

I

V

A

D

D

A

Q

L

L

V

I

I

V

G

G

G

G

G

G

M

I

A

A

N

N

T

T

F

F

L

I

A

A

A

A

R

Q

G

G

T

H

N

D

V

V

G

G

K

K

S

S

L

L

C

Y

E

E

H

A

D

D

L

L

A

V

D

D

T

E

A

A

K

K

Q

R

I

L

M

L

I

-

E

-

A

-

A

-

A

-

S

T

G

T

C

C

A

N

I

I

V

P

L

V

P

P

E

S

D

D

G

V

V

R

I

V

A

A

R

T

E

E

F

F

K

S

A

E

G

T

A

A

D

P

N

A

E

T

V

L

V

K

A

S

I

V

E

N

A

D

I

V

P

K

A

A

D

D

A

E

M

Q

V

I

L

L

D

D

V

I

G

G

P

D

K

A

S

S

V

A

E

Q

A

E

I

L

K

A

N

E

W

I

I

L

G

K

R

N

A

A

T

K

T

T

L

I

V

L

W

W

N

N

G

G

P

P

L

V

G

G

A

V

F

F

E

E

I

F

T

P

P

N

F

F

D

R

A

K

A

G

T

T

V

E

A

I

A

V

A

A

K

N

Y

A

A

I

A

A

E

D

C

S

T

-

K

-

A

-

G

-

K

E

L

A

T

F

S

S

V

I

A

A

G

G

G

G

G

G

D

D

T

T

V

L

S

A

A

A

L

I

N

D

H

L

A

F

G

G

V

I

A

A

D

D

D

K

F

I

T

S

Y

Y

V

I

S

S

T

T

A

G

G

G

G

G

A

A

F

F

L

L

E

E

W

F

M

V

E

E

G

G

K

K

V

V

L

L

P

P

G

A

V

V

A

A

V

M

L

L

Q

E

M1 (= M1) modified: Initiator methionine, Removed
K84 (≠ V85) modified: N6-acetyllysine

1zmrA Crystal structure of the e. Coli phosphoglycerate kinase (see paper)
44% identity, 97% coverage: 12:397/400 of query aligns to 9:381/386 of 1zmrA

query
sites
1zmrA

D

D

V

L

T

A

G

G

K

K

R

R

V

V

L

F

V

I

R

R

V

A

D

D

L

L

N

N

V

V

P

P

V

V

K

K

D

D

G

G

K

K

V

V

T

T

D

S

A

D

T

A

R
|
R

I

I

E

R

R

A

V

S

A

L

P

P

T

T

I

I

L

E

E

L

L

A

S

L

E

K

K

Q

G

G

A

A

K

K

V

V

I

M

L

V

L

T

A

S

H

H

F

L

G

G

R

R

P

P

K

T

D

E

G

G

P

E

-

Y

S

N

P

E

D

E

L

F

S

S

L

L

G

L

L

P

I

V

V

V

P

N

A

Y

V

L

E
x
K

E

D

V

K

L
|
L

D

S

H

N

A

P

V

V

L

R

F

L

A

V

S

K

D

D

C

Y

I

L

G

-

A

-

A

-

A

-

A

-

D

D

A

G

V

V

A

-

K

D

M

V

N

A

D

E

G

G

D

E

I

L

L

V

L

V

L

L

E

E

N

N

T

V

R

R

F

F

H

N

K

K

G

G

E

E

E

K

K

K

N

D

D

D

A

E

A

T

F

L

T

S

K

K

A

K

L

Y

A

A

A

A

N

L

G

C

D

D

L

V

Y

F

V

V

N

M

D

D

A

A

F

F

S

G

A

T

A

A

H

H

R

R

A

A

H

Q

C

A

S

S

T

T

E

H

G

G

L

I

A

G

Q

K

L

F

L

A

P

D

-

V

A

A

Y

C

A

A

G

G

R

P

T

L

M

L

Q

A

A

A

E

E

L

L

E

D