SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_004122049.1 NCBI__GCF_000359745.1:WP_004122049.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 2 hits to proteins with known functional sites (download)

P94530 Arabinooligosaccharides transport system permease protein AraQ from Bacillus subtilis (strain 168) (see paper)
30% identity, 88% coverage: 36:291/291 of query aligns to 27:281/281 of P94530

query
sites
P94530

L

L

F

F

P

P

I

I

Y

F

W

C

I

L

A

L

S

L

N

G

S

S

F

L

K

K

F

P

D

S

I

S

D

E

I

L

F

L

A

R

V

V

P

G

P

L

E

N

W

-

L

L

P

D

R

I

N

D

P

P

T

K

L

V

K

M

H

S

Y

F

D

D

E

N

-

Y

A

T

F

F

I

L

Q

F

R

N

P

G

-

G

-

S

-

I

F

Y

L

F

R

K

Y

W

A

F

L

F

N

N

S

S

F

L

L

V

V

L

A

G

V

L

G

F

T

T

T

T

V

V

V

L

S

T

V

L

T

F

F

F

G

S

T

S

M

M

A

I

G

G

Y

Y

A

G

L

L

A

A

R

-

F

-

S

V

Y

Y

P

D

W

F

Q

K

W

G

R

R

K

N

Q

I

I

I

S

F

F

V

W

L

I

V

L

L

S

I

T

I

R

M

M

M

M

V

P

P

P

L

I

E

V

V

S

M

I

M

I

L

P

P

L

L

Y

F

L

-

F

-

F

-

N

-

Y

-

F

-

D

-

M

K

L

L

N

T

T

V

K

G

S

L

A

H

L

L

I

I

V

D

A

S

Y

Y

T

T

A

G

F

V

N

I

L

L

P

P

F

F

-

I

-

V

-

S

-

P

-

V

A

A

T

V

W

F

M

F

M

F

K

R

S

Q

Y

Y

F

A

Q

L

D

G

L

L

P

P

V

R

E

D

L

L

E

L

E

D

A

S

A

A

I

R

V

M

D
|
D

G

G

D

C

T

T

R

E

W

F

G

G

A

I

F

F

L

F

H

R

V

I

A

M

L

A

P

P

L

L

A

M

R

K

P

P

G

A

L

F

A

G

A

A

T

M

A

I

I

I

F

L

C

Q

L

S

I

L

I

N

S

S

W

W

N

N

E

N

F

F

L

L

L

W

S

P

L

L

I

I

I

V

T

L

L

R

T

S

E

K

Q

E

S

M

Q

F

T

T

L

L

P

P

I

I

G

G

I

L

A

S

G

S

R

L

V

L

T

S

Q

P

Y

Y

N

G

T

N

Y

N

W

Y

G

D

E

M

I

L

S

I

A

S

A

G

G

S

F

V

M

F

A

A

C

I

V

L

P

P

I

V

V

I

I

I

F

I

A

F

F

L

I

F

V

F

Q

Q

K

K

H

Y

L

F

V

I

R

S

G

G

L

L

S

T

L

V

G

G

A

G

V

V

K

K

G

G

D180 (= D190) mutation to A: Negative impact in the growth rate in the presence of arabinotriose.

P68183 Maltose/maltodextrin transport system permease protein MalG from Escherichia coli (strain K12) (see 2 papers)
34% identity, 74% coverage: 78:291/291 of query aligns to 80:296/296 of P68183

query
sites
P68183

P

P

F

V

L

L

R

L

Y

W

A

L

L

W

N

N

S

S

F

V

L

K

V

V

A

A

V

G

G

I

T

S

T

A

V

I

V

G

S

I

V

V

T

A

F

L

G

S

T

T

M

T

A

C

G

A

Y

Y

A

A

L

F

A

A

R

R

F

M

S

R

Y

F

P

P

W

G

Q

-

W

-

R

K

K

A

Q

T

I

L

S

L

F

K

W

G

I

M

L

L

S

I

T

F

R

Q

M

M

M

F

P

P

P

A

I

V

V

L

S

S

I

L

I

V

P

A

L

L

Y

Y

L

A

F

L

F

F

N

D

Y

R

-

L

-

G

-

E

-

Y

-

I

-

P

F

F

D

I

M

G

L

L

N

N

T

T

K

H

S

G

A

G

L

V

I

I

V

F

A

A

Y

Y

T

L

A

G

F

-

N

G

L

I

P

A

F

L

A

H

T

V

W

W

M

T

M

I

K

K

S

G

Y

Y

F

F

Q

E

D

T

L

I

P

D

V

S

E

S

L

L

E

E

E
|
E

A

A

A
|
A

I

A

V

L

D

D

G
|
G

D

A

T

T

R

P

W

W

G

Q

A

A

F

F

L

R

H

L

V

V

A

L

L

L

P
|
P

L

L

A

S

R

V

P

P

G

I

L

L

A

A

A

V

T

V

A

F

I

I

F

L

C

S

L

F

I

I

I

A

S

A

W

I

N

T

E

E

F

V

L

P

L

V

S

A

L

S

I

L

I

L

T

L

L

R

T

D

E

V

Q

N

S

S

Q

Y

T

T

L

L

P

A

I

V

G

G

I

M

A

Q

G

Q

R

Y

V

L

T

N

Q

P

Y

Q

N

N

T

Y

Y

L

W

W

G

G

E

D

I

F

S

A

A

A

A

A

G

A

F

V

M

M

A

S

C

A

V

L

P

P

I

I

V

T

I

I

F

V

A

F

F

L

I

L

V

A

Q

Q

K

R

H

W

L

L

V

V

R

N

G

G

L

L

S

T

L

A

G

G

A

G

V

V

K

K

G

G

E190 (= E185) mutation E->A,C,K,L: Reduction of transport rate.
A192 (= A187) mutation A->D,S,L: Loss of transport and MalK dissociation from the membrane.
G196 (= G191) mutation to A: No effect.; mutation to P: Loss of transport and MalK dissociation from the membrane.
P209 (= P204) mutation to A: No effect.

Query Sequence

>WP_004122049.1 NCBI__GCF_000359745.1:WP_004122049.1
MTMTLAKTIHARSRTREMLIRLSAYLTISVALVVTLFPIYWIASNSFKFDIDIFAVPPEW
LPRNPTLKHYDEAFIQRPFLRYALNSFLVAVGTTVVSVTFGTMAGYALARFSYPWQWRKQ
ISFWILSTRMMPPIVSIIPLYLFFNYFDMLNTKSALIVAYTAFNLPFATWMMKSYFQDLP
VELEEAAIVDGDTRWGAFLHVALPLARPGLAATAIFCLIISWNEFLLSLIITLTEQSQTL
PIGIAGRVTQYNTYWGEISAAGFMACVPIVIFAFIVQKHLVRGLSLGAVKG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory