SitesBLAST

276 C→A: Lower ATPase activity and transport efficiency.
297 mutation E->K,D: Lower ATPase activity and transport efficiency.; E→Q: Loss of ATPase activity and transport.

3tuzC Inward facing conformations of the metni methionine abc transporter: cy5 semet soak crystal form (see paper)
35% identity, 88% coverage: 17:237/251 of query aligns to 10:228/344 of 3tuzC

query
sites
3tuzC

K

K

D

V

I
x
F

H

H

K
x
Q

S

G

F

T

D

R

A

T

I

I

V

Q

A

A

I

L

E

N

N

N

F

V

S

S

L

L

D

H

V

V

H

P

P

A

G

G

E

Q

I

I

V

Y

A

G

L

V

V

I

G

G

D

A

N

S

G
|
G

A

A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

I

I

K

R

I

C

I

V

S

N

G

L

V

L

Y

E

T

R

P

P

T

T

S

E

G

G

S

S

I

V

L

L

I

V

D

D

G

G

Q

Q

P

E

V

L

V

T

M

T

-

L

-

S

-

E

S

S

N

E

A

L

T

T

M

K

A

A

R

R

S

R

H

Q

G

-

I

I

E

G

V

M

V

I

Y

F

Q

Q

D

H

L

F

A

N

L

L

A

L

D

S

Q

S

Q

R

T

T

V

V

Y

F

M

G

N

N

M

V

F

A

L

L

G

P

R

L

E

E

P

-

T

-

K

-

S

-

F

-

G

-

L

-

L

L

D

D

R

N

-

T

-

P

-

K

-

D

-

E

-

V

K

K

K

R

M

R

I

V

A

T

E

E

T

L

E

L

A

S

L

L

V

V

K

G

E

L

L

G

D

D

V

K

R

H

-

D

-

S

I

Y

P

P

S

S

A

-

H

-

A

-

T

-

I

-

R

-

D

N

L

L

S

S

G

G

Q

Q

R

K

Q

Q

G

R

V

V

A

A

I

I

A

A

R

R

A

A

T

L

H

A

W

S

A

N

S

P

K

K

L

V

V

L

L

L

L

C

D

D

E

Q

P

A

T

T

A

S

A

A

L

L

G

D

V

P

A

A

E

T

T

T

A

R

K

S

V

I

E

L

E

E

I

L

V

L

E

K

S

D

L

I

K

N

K

R

R

R

-

L

N

G

I

L

G

T

I

I

L

L

I

L

I

I

S

T

H

H

S

E

L

M

D

D

Q

V

V

V

F

K

K

R

L

I

S

C

D

D

R

C

I

V

C

A

V

V

L

I

R

S

R

N

G

G

K

E

Q

L

I

I

G

E

V

Q

R

D

K

T

T

V

S

S

E

E

binding adenosine-5'-diphosphate: F12 (≠ I19), Q14 (≠ K21), G42 (= G49), G44 (= G51), K45 (= K52), S46 (= S53), T47 (= T54)

Sites not aligning to the query:

binding selenomethionine: 274, 279, 281, 282, 283, 284, 311, 313

3tuiC Inward facing conformations of the metni methionine abc transporter: cy5 native crystal form (see paper)
35% identity, 88% coverage: 17:237/251 of query aligns to 10:228/344 of 3tuiC

query
sites
3tuiC

K

K

D

V

I
x
F

H

H

K

Q

S

G

F

T

D

R

A

T

I

I

V

Q

A

A

I

L

E

N

N

N

F

V

S

S

L

L

D

H

V

V

H

P

P

A

G

G

E

Q

I

I

V

Y

A

G

L

V

V

I

G

G

D

A

N

S

G
|
G

A
|
A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

I

I

K

R

I

C

I

V

S

N

G

L

V

L

Y

E

T

R

P

P

T

T

S

E

G

G

S

S

I

V

L

L

I

V

D

D

G

G

Q

Q

P

E

V

L

V

T

M

T

-

L

-

S

-

E

S

S

N

E

A

L

T

T

M

K

A

A

R

R

S

R

H

Q

G

-

I

I

E

G

V

M

V

I

Y

F

Q

Q

D

H

L

F

A

N

L

L

A

L

D

S

Q

S

Q

R

T

T

V

V

Y

F

M

G

N

N

M

V

F

A

L

L

G

P

R

L

E

E

P

-

T

-

K

-

S

-

F

-

G

-

L

-

L

L

D

D

R

N

-

T

-

P

-

K

-

D

-

E

-

V

K

K

K

R

M

R

I

V

A

T

E

E

T

L

E

L

A

S

L

L

V

V

K

G

E

L

L

G

D

D

V

K

R

H

-

D

-

S

I

Y

P

P

S

S

A

-

H

-

A

-

T

-

I

-

R

-

D

N

L

L

S

S

G

G

Q

Q

R

K

Q

Q

G

R

V

V

A

A

I

I

A

A

R

R

A

A

T

L

H

A

W

S

A

N

S

P

K

K

L

V

V

L

L

L

L

C

D

D

E

Q

P

A

T

T

A

S

A

A

L

L

G

D

V

P

A

A

E

T

T

T

A

R

K

S

V

I

E

L

E

E

I

L

V

L

E

K

S

D

L

I

K

N

K

R

R

R

-

L

N

G

I

L

G

T

I

I

L

L

I

L

I

I

S

T

H

H

S

E

L

M

D

D

Q

V

V

V

F

K

K

R

L

I

S

C

D

D

R

C

I

V

C

A

V

V

L

I

R

S

R

N

G

G

K

E

Q

L

I

I

G

E

V

Q

R

D

K

T

T

V

S

S

E

E

binding adenosine-5'-diphosphate: F12 (≠ I19), G42 (= G49), A43 (= A50), G44 (= G51), K45 (= K52), S46 (= S53), T47 (= T54)

P07821 Iron(3+)-hydroxamate import ATP-binding protein FhuC; Ferric hydroxamate uptake protein C; Ferrichrome transport ATP-binding protein FhuC; Iron(III)-hydroxamate import ATP-binding protein FhuC; EC 7.2.2.16 from Escherichia coli (strain K12) (see 2 papers)
32% identity, 94% coverage: 16:250/251 of query aligns to 14:247/265 of P07821

query
sites
P07821

L

L

K

R

D

N

I

I

H

S

K

F

S

R

F

V

D

P

A

G

I

R

V

T

A

L

I

L

E

H

N

P

F

L

S

S

L

L

D

T

V

F

H

P

P

A

G

G

E

K

I

V

V

T

A

G

L

L

V

I

G

G

D

H

N

N

G

G

A

S

G

G

K
|
K

S

S

T

T

L

L

I

L

K

K

I

M

I

L

S

G

G

R

V

H

Y

Q

T

P

P

P

T

S

S

E

G

G

S

E

I

I

L

L

I

L

D

D

G

A

Q

Q

P

P

V

-

V

L

M

E

S

S

N

W

A

S

T

S

M

K

A

A

R

F

S

A

H

R

G

K

I

V

E

A

V

Y

V

L

Y

P

Q

Q

D

Q

L

L

A

P

L

P

A

A

D

E

Q

G

Q

M

T

T

V

V

Y

R

M

E

N

L

M

V

F

A

L

I

G

G

R

R

E

Y

P

P

T

W

K

H

S

G

-

A

-

L

-

G

-

R

F

F

G

G

L

A

D

D

R

R

K

E

K

K

M

-

I

V

A

E

E

E

T

A

E

I

A

S

L

L

V

V

K

G

E

-

L

-

D

-

V

-

R

L

I

K

P

P

S

L

A

A

H

H

A

R

T

L

I

V

R

D

D

S

L

L

S

S

G

G

Q

E

R

R

Q

Q

G

R

V

A

A

W

I

I

A

A

R

M

A

L

T

V

H

A

W

Q

A

D

S

S

K

R

L

C

V

L

L

L

D
|
D

E
|
E

P

P

T

T

A

S

A

A

L

L

G

D

V

I

A

A

E

H

T

Q

A

V

K

D

V

V

E

L

E

S

I

L

V

V

E

H

S

R

L

L

-

S

K

Q

K

E

R

R

N

G

I

L

G

T

I

V

L

I

I

A

I

V

S

L

H

H

S

D

L

I

D

N

Q

M

V

A

F

A

K

R

L

Y

S

C

D

D

R

Y

I

L

C

V

V

A

L

L

R

R

R

G

G

G

K

E

Q

M

I

I

G

A

V

Q

R

G

K

T

T

P

S

A

E

E

T

I

D

M

K

R

N

G

E

E

I

T

I

L

A

E

M

M

I

I

T

Y

G

G

L

I

K50 (= K52) mutation to Q: Lack of activity.
D172 (= D176) mutation to E: Lack of activity.
E173 (= E177) mutation to A: Lack of activity.

P75831 Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- from Escherichia coli (strain K12) (see paper)
32% identity, 86% coverage: 12:228/251 of query aligns to 3:220/648 of P75831

query
sites
P75831

P

P

V

L

L

L

E

E

L

L

K

K

D

D

I

I

H

R

K

R

S

S

F

Y

D

P

A

A

-

G

-

D

-

E

-

Q

I

V

V

E

A

V

I

L

E

K

N

G

F

I

S

S

L

L

D

D

V

I

H

Y

P

A

G

G

E

E

I

M

V

V

A

A

L

I

V

V

G

G

D

A

N

S

G

G

A

S

G

G

K
|
K

S

S

T

T

L

L

I

M

K

N

I

I

I

L

S

G

G

C

V

L

Y

D

T

K

P

A

T

T

S

S

G

G

S

T

I

Y

L

R

I

V

D

A

G

G

Q

Q

P

D

V

V

V

A

M

T

S

L

N

D

-

A

-

D

-

A

-

L

A

A

T

Q

M

L

A

R

R

R

S

E

H

H

G

-

I

F

E

G

V

F

V

I

Y

F

Q

Q

D

R

L

Y

A

H

L

L

A

L

D

S

Q

H

Q

L

T

T

V

A

Y

E

M

Q

N

N

M

V

F

-

L

-

G

-

R

-

E

E

P

V

T

P

K

A

S

V

F

Y

G

A

L

G

L

L

D

E

R

R

K

K

K

Q

M

R

I

L

A

L

E

R

T

A

E

Q

A

E

L

L

V

L

K

Q

E

R

L

L

D

G

V

L

R

E

I

D

P

R

S

T

A

E

H

Y

A

Y

T

P

I

A

R

Q

D

-

L

L

S

S

G

G

Q

Q

R

Q

Q

Q

G

R

V

V

A

S

I

I

A

A

R

R

A

A

T

L

H

M

W

N

A

G

S

G

K

Q

L

V

V

I

L

L

L

A

D
|
D

E

E

P

P

T

T

A

G

A

A

L

L

G

D

V

S

A

H

E

S

T

G

A

E

K

E

V

V

E

M

E

A

I

I

V

L

E

H

S

Q

L

L

K

R

K

D

R

R

N

G

I

H

G

T

I

V

L

I

I

I

I

V

S

T

H

H

S

D

L

-

D

P

Q

Q

V

V

F

A

K

A

L

Q

S

A

D

E

R

R

I

V

C

I

V

E

L

I

R

R

R

D

G

G

K

E

K47 (= K52) mutation to L: Lack of activity.
D169 (= D176) mutation to N: Lack of activity.

1oxvD Crystal structure of glcv, the abc-atpase of the glucose abc transporter from sulfolobus solfataricus (see paper)
29% identity, 94% coverage: 16:250/251 of query aligns to 6:240/353 of 1oxvD

query
sites
1oxvD

L

V

K

K

D

N

I

V

H

S

K

K

S

V

F
|
F

D

K

A

K

-

G

-

K

I

V

V

V

A

A

I

L

E

D

N

N

F

V

S

N

L

I

D

N

V

I

H

E

P

N

G

G

E

E

I

R

V

F

A

G

L

I

V

L

G

G

D

P

N
x
S

G
|
G

A

A

G
|
G

K
|
K

S
x
T

T
|
T

L

F

I

M

K

R

I

I

S

A

G

G

V

L

Y

D

T

V

P

P

T

S

S

T

G

G

S

E

I

L

L

Y

I

F

D

D

G

D

Q

R

P

-

V

L

V

V

M

A

S

S

N

N

A

G

T

K

M

L

-

I

-

V

-

P

A

P

R

E

S

D

H

R

G

K

I

I

E

G

V

M

V

V

Y

F

Q
|
Q

D

T

L

W

A

A

L

L

A

Y

D

P

Q

N

Q

L

T

T

V

A

Y

F

M

E

N

N

M

I

F

A

L

F

G

P

R

L

E

T

P

N

T

M

K

K

S

-

F

-

G

-

L

-

L

M

D

S

R

K

K

E

M

I

I

R

A

K

E

R

T

V

E

E

A

E

L

V

V

A

K

K

E

I

L

L

D

D

V

I

R

H

I

H

P

V

S

L

A

N

H

H

A

F

T

P

I

-

R

R

D

E

L

L

S

S

G

G

Q

Q

R

Q

Q

Q

G

R

V

V

A

A

I

L

A

A

R

R

A

A

T

L

H

V

W

K

A

D

S

P

K

S

L

L

V

L

L

L

D

D

E
|
E

P

P

T

F

A

S

A

N

L

L

G

D

V

A

A

R

E

M

T

R

A

D

K

S

V

A

E

R

E

A

I

L

V

V

E

K

S

E

L

V

K

Q

K

S

R

R

-

L

N

G

I

V

G

T

I

L

L

L

I

V

S

S

H

H

S

D

L

P

D

A

Q

D

V

I

F

F

K

A

L

I

S

A

D

D

R

R

I

V

C

G

V

V

L

L

R

V

R

K

G

G

K

K

Q

L

I

V

G

Q

V

V

R

G

K

K

T

P

S

E

E

D

T

L

D

Y

K

D

N

N

E

P

I

V

I

S

A

I

M

Q

I

V

T

A

G

S

L

L

binding phosphoaminophosphonic acid-adenylate ester: F13 (= F23), S40 (≠ N48), G41 (= G49), G43 (= G51), K44 (= K52), T45 (≠ S53), T46 (= T54), Q89 (= Q95), E166 (= E177)
binding magnesium ion: T45 (≠ S53), Q89 (= Q95)

1oxvA Crystal structure of glcv, the abc-atpase of the glucose abc transporter from sulfolobus solfataricus (see paper)
29% identity, 94% coverage: 16:250/251 of query aligns to 6:240/353 of 1oxvA

query
sites
1oxvA

L

V

K

K

D

N

I

V

H

S

K

K

S

V

F
|
F

D

K

A

K

-

G

-

K

I

V

V

V

A

A

I

L

E

D

N

N

F

V

S

N

L

I

D

N

V

I

H

E

P

N

G

G

E

E

I

R

V

F

A

G

L

I

V

L

G

G

D

P

N
x
S

G
|
G

A

A

G
|
G

K
|
K

S
x
T

T
|
T

L

F

I

M

K

R

I

I

S

A

G

G

V

L

Y

D

T

V

P

P

T

S

S

T

G

G

S

E

I

L

L

Y

I

F

D

D

G

D

Q

R

P

-

V

L

V

V

M

A

S

S

N

N

A

G

T

K

M

L

-

I

-

V

-

P

A

P

R

E

S

D

H

R

G

K

I

I

E

G

V

M

V

V

Y

F

Q

Q

D

T

L

W

A

A

L

L

A

Y

D

P

Q

N

Q

L

T

T

V

A

Y

F

M

E

N

N

M

I

F

A

L

F

G

P

R

L

E

T

P

N

T

M

K

K

S

-

F

-

G

-

L

-

L

M

D

S

R

K

K

E

M

I

I

R

A

K

E

R

T

V

E

E

A

E

L

V

V

A

K

K

E

I

L

L

D

D

V

I

R

H

I

H

P

V

S

L

A

N

H

H

A

F

T

P

I

-

R

R

D

E

L

L

S

S

G

G

Q

Q

R

Q

Q

Q

G

R

V

V

A

A

I

L

A

A

R

R

A

A

T

L

H

V

W

K

A

D

S

P

K

S

L

L

V

L

L

L

D

D

E
|
E

P

P

T

F

A

S

A

N

L

L

G

D

V

A

A

R

E

M

T

R

A

D

K

S

V

A

E

R

E

A

I

L

V

V

E

K

S

E

L

V

K

Q

K

S

R

R

-

L

N

G

I

V

G

T

I

L

L

L

I

V

S

S

H

H

S

D

L

P

D

A

Q

D

V

I

F

F

K

A

L

I

S

A

D

D

R

R

I

V

C

G

V

V

L

L

R

V

R

K

G

G

K

K

Q

L

I

V

G

Q

V

V

R

G

K

K

T

P

S

E

E

D

T

L

D

Y

K

D

N

N

E

P

I

V

I

S

A

I

M

Q

I

V

T

A

G

S

L

L

binding phosphoaminophosphonic acid-adenylate ester: F13 (= F23), S40 (≠ N48), G41 (= G49), G43 (= G51), K44 (= K52), T45 (≠ S53), T46 (= T54), E166 (= E177)

1oxuA Crystal structure of glcv, the abc-atpase of the glucose abc transporter from sulfolobus solfataricus (see paper)
29% identity, 94% coverage: 16:250/251 of query aligns to 6:240/353 of 1oxuA

query
sites
1oxuA

L

V

K

K

D

N

I

V

H

S

K

K

S

V

F
|
F

D

K

A

K

-

G

-

K

I

V

V

V

A
|
A

I

L

E

D

N

N

F

V

S

N

L

I

D

N

V

I

H

E

P

N

G

G

E

E

I

R

V

F

A

G

L

I

V

L

G

G

D

P

N

S

G
|
G

A

A

G
|
G

K
|
K

S
x
T

T
|
T

L

F

I

M

K

R

I

I

S

A

G

G

V

L

Y

D

T

V

P

P

T

S

S

T

G

G

S

E

I

L

L

Y

I

F

D

D

G

D

Q

R

P

-

V

L

V

V

M

A

S

S

N

N

A

G

T

K

M

L

-

I

-

V

-

P

A

P

R

E

S

D

H

R

G

K

I

I

E

G

V

M

V

V

Y

F

Q

Q

D

T

L

W

A

A

L

L

A

Y

D

P

Q

N

Q

L

T

T

V

A

Y

F

M

E

N

N

M

I

F

A

L

F

G

P

R

L

E

T

P

N

T

M

K

K

S

-

F

-

G

-

L

-

L

M

D

S

R

K

K

E

M

I

I

R

A

K

E

R

T

V

E

E

A

E

L

V

V

A

K

K

E

I

L

L

D

D

V

I

R

H

I

H

P

V

S

L

A

N

H

H

A

F

T

P

I

-

R

R

D

E

L

L

S

S

G

G

Q

Q

R

Q

Q

Q

G

R

V

V

A

A

I

L

A

A

R

R

A

A

T

L

H

V

W

K

A

D

S

P

K

S

L

L

V

L

L

L

D

D

E

E

P

P

T

F

A

S

A

N

L

L

G

D

V

A

A

R

E

M

T

R

A

D

K

S

V

A

E

R

E

A

I

L

V

V

E

K

S

E

L

V

K

Q

K

S

R

R

-

L

N

G

I

V

G

T

I

L

L

L

I

V

S

S

H

H

S

D

L

P

D

A

Q

D

V

I

F

F

K

A

L

I

S

A

D

D

R

R

I

V

C

G

V

V

L

L

R

V

R

K

G

G

K

K

Q

L

I

V

G

Q

V

V

R

G

K

K

T

P

S

E

E

D

T

L

D

Y

K

D

N

N

E

P

I

V

I

S

A

I

M

Q

I

V

T

A

G

S

L

L

binding adenosine-5'-diphosphate: F13 (= F23), A20 (= A28), G41 (= G49), G43 (= G51), K44 (= K52), T45 (≠ S53), T46 (= T54)

Q97UY8 Glucose import ATP-binding protein GlcV; EC 7.5.2.- from Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus) (see paper)
29% identity, 94% coverage: 16:250/251 of query aligns to 6:240/353 of Q97UY8

query
sites
Q97UY8

L

V

K

K

D

N

I

V

H

S

K

K

S

V

F

F

D

K

A

K

-

G

-

K

I

V

V

V

A

A

I

L

E

D

N

N

F

V

S

N

L

I

D

N

V

I

H

E

P

N

G

G

E

E

I

R

V

F

A

G

L

I

V

L

G

G

D

P

N

S

G

G

A

A

G

G

K

K

S

T

T

T

L

F

I

M

K

R

I

I

S

A

G

G

V

L

Y

D

T

V

P

P

T

S

S

T

G

G

S

E

I

L

L

Y

I

F

D

D

G

D

Q

R

P

-

V

L

V

V

M

A

S

S

N

N

A

G

T

K

M

L

-

I

-

V

-

P

A

P

R

E

S

D

H

R

G

K

I

I

E

G

V

M

V

V

Y

F

Q

Q

D

T

L

W

A

A

L

L

A

Y

D

P

Q

N

Q

L

T

T

V

A

Y

F

M

E

N

N

M

I

F

A

L

F

G

P

R

L

E

T

P

N

T

M

K

K

S

-

F

-

G

-

L

-

L

M

D

S

R

K

K

E

M

I

I

R

A

K

E

R

T

V

E

E

A

E

L

V

V

A

K

K

E

I

L

L

D

D

V

I

R

H

I

H

P

V

S

L

A

N

H

H

A

F

T

P

I

-

R

R

D

E

L

L

S
|
S

G

G

G
|
G

Q

Q

R

Q

Q

Q

G

R

V

V

A

A

I

L

A

A

R

R

A

A

T

L

H

V

W

K

A

D

S

P

K

S

L

L

V

L

L

L

D

D

E
|
E

P

P

T

F

A

S

A

N

L

L

G

D

V

A

A

R

E

M

T

R

A

D

K

S

V

A

E

R

E

A

I

L

V

V

E

K

S

E

L

V

K

Q

K

S

R

R

-

L

N

G

I

V

G

T

I

L

L

L

I

V

S

S

H

H

S

D

L

P

D

A

Q

D

V

I

F

F

K

A

L

I

S

A

D

D

R

R

I

V

C

G

V

V

L

L

R

V

R

K

G

G

K

K

Q

L

I

V

G

Q

V

V

R

G

K

K

T

P

S

E

E

D

T

L

D

Y

K

D

N

N

E

P

I

V

I

S

A

I

M

Q

I

V

T

A

G

S

L

L

S142 (= S153) mutation to A: Decrease in ATPase activity. Can form dimers.
G144 (= G155) mutation to A: Loss of ATPase activity. Cannot form dimers. Forms an active heterodimer; when associated with A-166.
E166 (= E177) mutation to A: Loss of ATPase activity. Can form dimers in the presence of ATP-Mg(2+). Forms an active heterodimer; when associated with A-144.; mutation to Q: Strong decrease in ATPase activity. Can form dimers in the presence of ATP alone, without Mg(2+).

2awnB Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
35% identity, 89% coverage: 14:237/251 of query aligns to 3:219/374 of 2awnB

query
sites
2awnB

L

V

E

Q

L

L

K

Q

D

N

I

V

H

T

K

K

S

A

F
x
W

D

G

A

E

I

V

V

V

A
x
V

I

S

E

K

N

D

F

I

S

N

L

L

D

D

V

I

H

H

P

E

G

G

E

E

I

F

V

V

A

V

L

F

V

V

G

G

D

P

N
x
S

G
|
G

A

C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

I

L

K

R

I

M

I

I

S

A

G

G

V

L

Y

E

T

T

P

I

T

T

S

S

G

G

S

D

I

L

L

F

I

I

D

G

G

E

Q

K

P

R

V

M

V

-

M

-

S

-

N

N

A

D

T

T

M

P

A

P

R

A

S

E

H

R

G

G

I

V

E

G

V

M

V

V

Y

F

Q

Q

D

S

L

Y

A

A

L

L

A

Y

D

P

Q

H

Q

L

T

S

V

V

Y

A

M

E

N

N

M

M

F

S

L

F

G

G

R

L

E

K

-

L

-

A

P

G

T

A

K

K

S

K

F

E

G

V

L

I

L

N

D

Q

R

R

K

V

K

N

M

Q

I

V

A

A

E

E

T

V

E

L

A

Q

L

L

V

A

K

H

E

L

L

L

D

D

V

-

R

R

I

K

P

P

S

K

A

A

H

-

A

-

T

-

I

-

R

-

D

-

L

L

S

S

G

G

Q

Q

R

R

Q

Q

G

R

V

V

A

A

I

I

A

G

R

R

A

T

T

L

H

V

W

A

A

E

S

P

K

S

L

V

V

F

L

L

D

D

E

E

P

P

T

L

A

S

A

N

L

L

G

D

V

A

A

A

E

L

T

R

A

V

K

Q

V

M

E

R

E

I

I

E

V

I

E

S

S

R

L

L

K

H

K

K

R

R

N

-

I

L

G

G

I

R

L

T

I

M

I

I

S

Y

H

V

S

T

L

H

D

D

Q

Q

V

V

-

E

-

A

F

M

K

T

L

L

S

A

D

D

R

K

I

I

C

V

V

V

L

L

R

D

R

A

G

G

K

R

Q

V

I

A

G

Q

V

V

R

G

K

K

T

P

S

L

E

E

binding adenosine-5'-diphosphate: W12 (≠ F23), V17 (≠ A28), S37 (≠ N48), G38 (= G49), G40 (= G51), K41 (= K52), S42 (= S53), T43 (= T54)

1q12A Crystal structure of the atp-bound e. Coli malk (see paper)
35% identity, 89% coverage: 14:237/251 of query aligns to 1:217/367 of 1q12A

query
sites
1q12A

L

V

E

Q

L

L

K

Q

D

N

I

V

H

T

K

K

S

A

F
x
W

D

G

A

E

I

V

V

V

A

V

I

S

E

K

N

D

F

I

S

N

L

L

D

D

V

I

H

H

P

E

G

G

E

E

I

F

V

V

A

V

L

F

V

V

G

G

D

P

N
x
S

G
|
G

A
x
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

I

L

K

R

I

M

I

I

S

A

G

G

V

L

Y

E

T

T

P

I

T

T

S

S

G

G

S

D

I

L

L

F

I

I

D

G

G

E

Q

K

P

R

V

M

V

-

M

-

S

-

N

N

A

D

T

T

M

P

A

P

R

A

S

E

H

R

G

G

I

V

E

G

V

M

V

V

Y

F

Q

Q

D

S

L

Y

A

A

L

L

A

Y

D

P

Q

H

Q

L

T

S

V

V

Y

A

M

E

N

N

M

M

F

S

L

F

G

G

R

L

E

K

-

L

-

A

P

G

T

A

K

K

S

K

F

E

G

V

L

I

L

N

D

Q

R

R

K

V

K

N

M

Q

I

V

A

A

E

E

T

V

E

L

A

Q

L

L

V

A

K

H

E

L

L

L

D

D

V

-

R
|
R

I

K

P

P

S

K

A
|
A

H

-

A

-

T

-

I

-

R

-

D

-

L

L

S
|
S

G

G

G
|
G

Q
|
Q

R

R

Q

Q

G

R

V

V

A

A

I

I

A

G

R

R

A

T

T

L

H

V

W

A

A

E

S

P

K

S

L

V

V

F

L

L

D

D

E

E

P

P

T

L

A

S

A

N

L

L

G

D

V

A

A

A

E

L

T

R

A

V

K

Q

V

M

E

R

E

I

I

E

V

I

E

S

S

R

L

L

K

H

K

K

R

R

N

-

I

L

G

G

I

R

L

T

I

M

I

I

S

Y

H

V

S

T

L

H

D

D

Q

Q

V

V

-

E

-

A

F

M

K

T

L

L

S

A

D

D

R

K

I

I

C

V

V

V

L

L

R

D

R

A

G

G

K

R

Q

V

I

A

G

Q

V

V

R

G

K

K

T

P

S

L

E

E

binding adenosine-5'-triphosphate: W10 (≠ F23), S35 (≠ N48), G36 (= G49), C37 (≠ A50), G38 (= G51), K39 (= K52), S40 (= S53), T41 (= T54), R126 (= R141), A130 (= A145), S132 (= S153), G134 (= G155), Q135 (= Q156)

P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
35% identity, 89% coverage: 14:237/251 of query aligns to 4:220/371 of P68187

query
sites
P68187

L

V

E

Q

L

L

K

Q

D

N

I

V

H

T

K

K

S

A

F

W

D

G

A

E

I

V

V

V

A

V

I

S

E

K

N

D

F

I

S

N

L

L

D

D

V

I

H

H

P

E

G

G

E

E

I

F

V

V

A

V

L

F

V

V

G

G

D

P

N

S

G

G

A

C

G

G

K

K

S

S

T

T

L

L

I

L

K

R

I

M

I

I

S

A

G

G

V

L

Y

E

T

T

P

I

T

T

S

S

G

G

S

D

I

L

L

F

I

I

D

G

G

E

Q

K

P

R

V

M

V

-

M

-

S

-

N

N

A

D

T

T

M

P

A

P

R

A

S

E

H

R

G

G

I

V

E

G

V

M

V

V

Y

F

Q

Q

D

S

L

Y

A
|
A

L

L

A

Y

D

P

Q

H

Q

L

T

S

V

V

Y

A

M

E

N

N

M

M

F

S

L

F

G

G

R

L

E

K

-

L

-

A

P

G

T

A

K
|
K

S

K

F

E

G

V

L

I

L

N

D

Q

R

R

K
x
V

K

N

M

Q

I
x
V

A

A

E
|
E

T

V

E

L

A

Q

L

L

V
x
A

K

H

E

L

L

L

D

D

V

-

R

R

I

K

P

P

S

K

A

A

H

-

A

-

T

-

I

-

R

-

D

-

L

L

S

S

G

G

G
|
G

Q

Q

R

R

Q

Q

G

R

V

V

A

A

I

I

A

G

R

R

A

T

T

L

H

V

W

A

A

E

S

P

K

S

L

V

V

F

L

L

D
|
D

E

E

P

P

T

L

A

S

A

N

L

L

G

D

V

A

A

A

E

L

T

R

A

V

K

Q

V

M

E

R

E

I

I

E

V

I

E

S

S

R

L

L

K

H

K

K

R

R

N

-

I

L

G

G

I

R

L

T

I

M

I

I

S

Y

H

V

S

T

L

H

D

D

Q

Q

V

V

-

E

-

A

F

M

K

T

L

L

S

A

D

D

R

K

I

I

C

V

V

V

L

L

R

D

R

A

G

G

K

R

Q

V

I

A

G

Q

V

V

R

G

K

K

T

P

S

L

E

E

A85 (= A98) mutation to M: Suppressor of EAA loop mutations in MalFG.
K106 (= K117) mutation to C: Suppressor of EAA loop mutations in MalFG.
V114 (≠ K125) mutation to C: Suppressor of EAA loop mutations in MalFG.
V117 (≠ I128) mutation to M: Suppressor of EAA loop mutations in MalFG.
E119 (= E130) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
A124 (≠ V135) mutation to T: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
G137 (= G155) mutation to A: Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type MalK.
D158 (= D176) mutation to N: Loss of maltose transport but retains ability to repress mal genes.

Sites not aligning to the query:

228 R→C: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
241 F→I: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
267 W→G: Normal maltose transport but constitutive mal gene expression.
278 G→P: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
282 S→L: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
284 G→S: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
302 G→D: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
308 E→Q: Maltose transport is affected but retains ability to interact with MalT.
322 S→F: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
340 G→A: Maltose transport is affected but retains ability to interact with MalT.
346 G→S: Normal maltose transport but constitutive mal gene expression.
355 F→Y: Maltose transport is affected but retains ability to interact with MalT.

3puyA Crystal structure of an outward-facing mbp-maltose transporter complex bound to amp-pnp after crystal soaking of the pretranslocation state (see paper)
35% identity, 89% coverage: 14:237/251 of query aligns to 3:219/371 of 3puyA

query
sites
3puyA

L

V

E

Q

L

L

K

Q

D

N

I

V

H

T

K

K

S

A

F
x
W

D

G

A

E

I

V

V

V

A

V

I

S

E

K

N

D

F

I

S

N

L

L

D

D

V

I

H

H

P

E

G

G

E

E

I

F

V

V

A

V

L

F

V

V

G

G

D

P

N
x
S

G
|
G

A
x
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

I

L

K

R

I

M

I

I

S

A

G

G

V

L

Y

E

T

T

P

I

T

T

S

S

G

G

S

D

I

L

L

F

I

I

D

G

G

E

Q

K

P

R

V

M

V

-

M

-

S

-

N

N

A

D

T

T

M

P

A

P

R

A

S

E

H

R

G

G

I

V

E

G

V

M

V

V

Y

F

Q
|
Q

D

S

L

Y

A

A

L

L

A

Y

D

P

Q

H

Q

L

T

S

V

V

Y

A

M

E

N

N

M

M

F

S

L

F

G

G

R

L

E

K

-

L

-

A

P

G

T

A

K

K

S

K

F

E

G

V

L

I

L

N

D

Q

R

R

K

V

K

N

M

Q

I

V

A

A

E

E

T

V

E

L

A

Q

L

L

V

A

K

H

E

L

L

L

D

D

V

-

R
|
R

I

K

P

P

S

K

A
|
A

H

-

A

-

T

-

I

-

R

-

D

-

L

L

S
|
S

G

G

G
|
G

Q
|
Q

R

R

Q

Q

G

R

V

V

A

A

I

I

A

G

R

R

A

T

T

L

H

V

W

A

A

E

S

P

K

S

L

V

V

F

L

L

D

D

E
|
E

P

P

T

L

A

S

A

N

L

L

G

D

V

A

A

A

E

L

T

R

A

V

K

Q

V

M

E

R

E

I

I

E

V

I

E

S

S

R

L

L

K

H

K

K

R

R

N

-

I

L

G

G

I

R

L

T

I

M

I

I

S

Y

H

V

S

T

L
x
H

D

D

Q

Q

V

V

-

E

-

A

F

M

K

T

L

L

S

A

D

D

R

K

I

I

C

V

V

V

L

L

R

D

R

A

G

G

K

R

Q

V

I

A

G

Q

V

V

R

G

K

K

T

P

S

L

E

E

binding phosphoaminophosphonic acid-adenylate ester: W12 (≠ F23), S37 (≠ N48), G38 (= G49), C39 (≠ A50), G40 (= G51), K41 (= K52), S42 (= S53), T43 (= T54), Q81 (= Q95), R128 (= R141), A132 (= A145), S134 (= S153), G136 (= G155), Q137 (= Q156), E158 (= E177), H191 (≠ L211)
binding magnesium ion: S42 (= S53), Q81 (= Q95)

3puxA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-bef3 (see paper)
35% identity, 89% coverage: 14:237/251 of query aligns to 3:219/371 of 3puxA

query
sites
3puxA

L

V

E

Q

L

L

K

Q

D

N

I

V

H

T

K

K

S

A

F
x
W

D

G

A

E

I

V

V

V

A

V

I

S

E

K

N

D

F

I

S

N

L

L

D

D

V

I

H

H

P

E

G

G

E

E

I

F

V

V

A

V

L

F

V

V

G

G

D

P

N
x
S

G
|
G

A
x
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

I

L

K

R

I

M

I

I

S

A

G

G

V

L

Y

E

T

T

P

I

T

T

S

S

G

G

S

D

I

L

L

F

I

I

D

G

G

E

Q

K

P

R

V

M

V

-

M

-

S

-

N

N

A

D

T

T

M

P

A

P

R

A

S

E

H

R

G

G

I

V

E

G

V

M

V

V

Y

F

Q
|
Q

D

S

L

Y

A

A

L

L

A

Y

D

P

Q

H

Q

L

T

S

V

V

Y

A

M

E

N

N

M

M

F

S

L

F

G

G

R

L

E

K

-

L

-

A

P

G

T

A

K

K

S

K

F

E

G

V

L

I

L

N

D

Q

R

R

K

V

K

N

M

Q

I

V

A

A

E

E

T

V

E

L

A

Q

L

L

V

A

K

H

E

L

L

L

D

D

V

-

R
|
R

I

K

P

P

S

K

A

A

H

-

A

-

T

-

I

-

R

-

D

-

L

L

S
|
S

G

G

G
|
G

Q
|
Q

R

R

Q

Q

G

R

V

V

A

A

I

I

A

G

R

R

A

T

T

L

H

V

W

A

A

E

S

P

K

S

L

V

V

F

L

L

D

D

E

E

P

P

T

L

A

S

A

N

L

L

G

D

V

A

A

A

E

L

T

R

A

V

K

Q

V

M

E

R

E

I

I

E

V

I

E

S

S

R

L

L

K

H

K

K

R

R

N

-

I

L

G

G

I

R

L

T

I

M

I

I

S

Y

H

V

S

T

L
x
H

D

D

Q

Q

V

V

-

E

-

A

F

M

K

T

L

L

S

A

D

D

R

K

I

I

C

V

V

V

L

L

R

D

R

A

G

G

K

R

Q

V

I

A

G

Q

V

V

R

G

K

K

T

P

S

L

E

E

binding adenosine-5'-diphosphate: W12 (≠ F23), G38 (= G49), C39 (≠ A50), G40 (= G51), K41 (= K52), S42 (= S53), T43 (= T54), R128 (= R141), S134 (= S153), Q137 (= Q156)
binding beryllium trifluoride ion: S37 (≠ N48), G38 (= G49), K41 (= K52), Q81 (= Q95), S134 (= S153), G136 (= G155), H191 (≠ L211)
binding magnesium ion: S42 (= S53), Q81 (= Q95)

3puwA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-alf4 (see paper)
35% identity, 89% coverage: 14:237/251 of query aligns to 3:219/371 of 3puwA

query
sites
3puwA

L

V

E

Q

L

L

K

Q

D

N

I

V

H

T

K

K

S

A

F
x
W

D

G

A

E

I

V

V

V

A
x
V

I

S

E

K

N

D

F

I

S

N

L

L

D

D

V

I

H

H

P

E

G

G

E

E

I

F

V

V

A

V

L

F

V

V

G

G

D

P

N
x
S

G
|
G

A
x
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

I

L

K

R

I

M

I

I

S

A

G

G

V

L

Y

E

T

T

P

I

T

T

S

S

G

G

S

D

I

L

L

F

I

I

D

G

G

E

Q

K

P

R

V

M

V

-

M

-

S

-

N

N

A

D

T

T

M

P

A

P

R

A

S

E

H

R

G

G

I

V

E

G

V

M

V

V

Y

F

Q
|
Q

D

S

L

Y

A

A

L

L

A

Y

D

P

Q

H

Q

L

T

S

V

V

Y

A

M

E

N

N

M

M

F

S

L

F

G

G

R

L

E

K

-

L

-

A

P

G

T

A

K

K

S

K

F

E

G

V

L

I

L

N

D

Q

R

R

K

V

K

N

M

Q

I

V

A

A

E

E

T

V

E

L

A

Q

L

L

V

A

K

H

E

L

L

L

D

D

V

-

R
|
R

I

K

P

P

S

K

A
|
A

H

-

A

-

T

-

I

-

R

-

D

-

L

L

S
|
S

G
|
G

Q
|
Q

R

R

Q

Q

G

R

V

V

A

A

I

I

A

G

R

R

A

T

T

L

H

V

W

A

A

E

S

P

K

S

L

V

V

F

L

L

D

D

E
|
E

P

P

T

L

A

S

A

N

L

L

G

D

V

A

A

A

E

L

T

R

A

V

K

Q

V

M

E

R

E

I

I

E

V

I

E

S

S

R

L

L

K

H

K

K

R

R

N

-

I

L

G

G

I

R

L

T

I

M

I

I

S

Y

H

V

S

T

L
x
H

D

D

Q

Q

V

V

-

E

-

A

F

M

K

T

L

L

S

A

D

D

R

K

I

I

C

V

V

V

L

L

R

D

R

A

G

G

K

R

Q

V

I

A

G

Q

V

V

R

G

K

K

T

P

S

L

E

E

binding adenosine-5'-diphosphate: W12 (≠ F23), V17 (≠ A28), G38 (= G49), C39 (≠ A50), G40 (= G51), K41 (= K52), S42 (= S53), T43 (= T54), R128 (= R141), A132 (= A145), S134 (= S153), Q137 (= Q156)
binding tetrafluoroaluminate ion: S37 (≠ N48), G38 (= G49), K41 (= K52), Q81 (= Q95), S134 (= S153), G135 (= G154), G136 (= G155), E158 (= E177), H191 (≠ L211)
binding magnesium ion: S42 (= S53), Q81 (= Q95)

3puvA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-vo4 (see paper)
35% identity, 89% coverage: 14:237/251 of query aligns to 3:219/371 of 3puvA

query
sites
3puvA

L

V

E

Q

L

L

K

Q

D

N

I

V

H

T

K

K

S

A

F
x
W

D

G

A

E

I

V

V

V

A
x
V

I

S

E

K

N

D

F

I

S

N

L

L

D

D

V

I

H

H

P

E

G

G

E

E

I

F

V

V

A

V

L

F

V

V

G

G

D

P

N

S

G
|
G

A
x
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

I

L

K

R

I

M

I

I

S

A

G

G

V

L

Y

E

T

T

P

I

T

T

S

S

G

G

S

D

I

L

L

F

I

I

D

G

G

E

Q

K

P

R

V

M

V

-

M

-

S

-

N

N

A

D

T

T

M

P

A

P

R

A

S

E

H

R

G

G

I

V

E

G

V

M

V

V

Y

F

Q
|
Q

D

S

L

Y

A

A

L

L

A

Y

D

P

Q

H

Q

L

T

S

V

V

Y

A

M

E

N

N

M

M

F

S

L

F

G

G

R

L

E

K

-

L

-

A

P

G

T

A

K

K

S

K

F

E

G

V

L

I

L

N

D

Q

R

R

K

V

K

N

M

Q

I

V

A

A

E

E

T

V

E

L

A

Q

L

L

V

A

K

H

E

L

L

L

D

D

V

-

R
|
R

I

K

P

P

S

K

A
|
A

H

-

A

-

T

-

I

-

R

-

D

-

L

L

S
|
S

G

G

Q
|
Q

R

R

Q

Q

G

R

V

V

A

A

I

I

A

G

R

R

A

T

T

L

H

V

W

A

A

E

S

P

K

S

L

V

V

F

L

L

D

D

E

E

P

P

T

L

A

S

A

N

L

L

G

D

V

A

A

A

E

L

T

R

A

V

K

Q

V

M

E

R

E

I

I

E

V

I

E

S

S

R

L

L

K

H

K

K

R

R

N

-

I

L

G

G

I

R

L

T

I

M

I

I

S

Y

H

V

S

T

L

H

D

D

Q

Q

V

V

-

E

-

A

F

M

K

T

L

L

S

A

D

D

R

K

I

I

C

V

V

V

L

L

R

D

R

A

G

G

K

R

Q

V

I

A

G

Q

V

V

R

G

K

K

T

P