SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_004128094.1 NCBI__GCF_000359745.1:WP_004128094.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 7 hits to proteins with known functional sites (download)

H1ZZB0 Aurachin B dehydrogenase; EC 1.1.1.394 from Stigmatella aurantiaca (see paper)
27% identity, 72% coverage: 1:211/295 of query aligns to 1:217/334 of H1ZZB0

query
sites
H1ZZB0

M

M

R

R

I

T

F

F

V

V

T

T

G

G

A

G

T

S

G

G

N

Y

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E

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R

S111 (≠ A102) mutation to A: Does not produce aurachin B.
Y139 (≠ S131) mutation to F: Does not produce aurachin B.

2pzmA Crystal structure of the bordetella bronchiseptica enzyme wbmg in complex with NAD and udp (see paper)
22% identity, 94% coverage: 1:278/295 of query aligns to 8:304/316 of 2pzmA

query
sites
2pzmA

M

M

R

R

I

I

F

L

V

I

T

T

G
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G

A

G

T

A

G
|
G

N
x
C

I
x
L

G

G

S

S

N

N

V

L

V

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R

E

E

H

L

W

I

L

A

P

A

Q

G

G

H

H

Q

E

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L

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S
x
D

R
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N

-
x
F

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x
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K
x
G

E

K

K

R

E

E

A

V

A

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L

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A

P

A

V

A

A

G

G

A

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E

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D

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G

active site: T123 (= T111), Y147 (≠ H134), K151 (≠ A138), P176 (= P164)
binding nicotinamide-adenine-dinucleotide: G14 (= G7), G17 (= G10), C18 (≠ N11), L19 (≠ I12), D38 (≠ S31), N39 (≠ R32), F40 (vs. gap), T42 (≠ S33), G43 (≠ K34), S60 (≠ T51), V61 (≠ I52), S82 (≠ L71), A83 (= A72), A84 (≠ F73), Y86 (≠ H75), T98 (≠ D86), F121 (≠ S109), Q122 (≠ G110), T123 (= T111), Y147 (≠ H134), K151 (≠ A138), V173 (= V161)
binding uridine-5'-diphosphate: F197 (≠ V182), C198 (= C183), S199 (≠ A184), R203 (≠ E187), I238 (≠ M228), D266 (≠ E250)

3aw9A Structure of udp-galactose 4-epimerase mutant
24% identity, 96% coverage: 1:282/295 of query aligns to 1:286/304 of 3aw9A

query
sites
3aw9A

M

M

R

R

I

I

F

V

V

V

T

T

G
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G

A

G

T

A

G
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G

N
x
F

I
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I

G

G

S

S

N

H

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L

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D

E

K

L

L

I

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A

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G

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L

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G

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L

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x
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D

E

-

A

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A

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L

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A

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E

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K

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G

D

D

G

V

V

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I

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x
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A
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A

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A

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N

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x
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E

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N

I

C

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D

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E

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N

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V

I

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A

A

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T

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L

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A

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A

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G

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K

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E

E

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x
V

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E

D

Y

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F

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G

G

W
|
W

L

P

S

G

M

-

F

-

A
x
D

T

V

R

K

D

Y

M

M

P

T

A

L

S

A

S

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A

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K

K

T

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Q

K

K

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G

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P

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T

active site: A105 (≠ T108), S107 (≠ G110), S108 (≠ T111), T109 (≠ P112), Y131 (≠ S131), K135 (≠ P135), L166 (≠ V165), G169 (= G168)
binding galactose-uridine-5'-diphosphate: P69 (≠ H75), V71 (≠ F77), S107 (≠ G110), Y131 (≠ S131), N160 (≠ Q160), H168 (≠ Q167), V170 (≠ L169), D173 (≠ P172), L188 (≠ I186), Q193 (≠ N191), K195 (≠ W193), Y197 (≠ A195), V234 (≠ M228), W263 (= W257), D266 (≠ A262)
binding nicotinamide-adenine-dinucleotide: G7 (= G7), G10 (= G10), F11 (≠ N11), I12 (= I12), D31 (≠ S31), I32 (≠ R32), R46 (≠ E53), D47 (= D54), L48 (≠ I55), F65 (≠ L71), A66 (= A72), A67 (≠ F73), E82 (≠ R88), A105 (≠ T108), S106 (= S109), Y131 (≠ S131), K135 (≠ P135), Y158 (≠ L158), N160 (≠ Q160), V161 (= V161), H168 (≠ Q167)

1r6dA Crystal structure of desiv double mutant (dtdp-glucose 4,6- dehydratase) from streptomyces venezuelae with NAD and dau bound (see paper)
24% identity, 74% coverage: 1:219/295 of query aligns to 1:241/322 of 1r6dA

query
sites
1r6dA

M

M

R

R

I

L

F

L

V

V

T

T

G

G

A

G

T

A

G
|
G

N
x
F

I
|
I

G

G

S

S

N

H

V

F

V

V

R

R

E

Q

L

L

I

L

A

A

A

G

G

A

H

Y

Q

P

-

D

-

V

-

P

-

A

-

D

-

E

-

V

-

I

V

V

L

L

G
x
D

L
x
S

S
x
L

R
x
T

S

Y

K
x
A

E
x
G

K

N

E

R

A

A

A

N

L

L

A

A

A

P

A

V

G

D

A

A

E

D

-

P

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R

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L

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R

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F

L

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H

G

G

T
x
D

I
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D

I

A

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I

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L

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K

A

S

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A

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D

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A

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I

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H

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A
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A

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D
x
H

F

V

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D

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R

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S

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A

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F

F

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E

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x
T

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N

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D

G

D

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R

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I

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A

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G

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x
Y

A

A

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x
K

E

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A

G

A

S

I

D

L

L

A

V

S

A

E

R

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A

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Y

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H

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T

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G

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D

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P

C

Q
x
N

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x
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H

Y

D

G

P

P

V

Y

S

Q

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H

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P

Q

E

G
x
K

L
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L

I

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P

P

L

L

F

I

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T

I

N

F

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K

G

G

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Y

E

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D

D

G

G

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A

N

N

R

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W
x
R

P

E

A

W

A

V

H

H

F

T

S

D

D

D

V

H

A

C

R

R

L

G

Y

I

R

A

L

L

V

V

V

L

E

A

R

G

A

G

E

R

P

A

N

G

A

E

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I

Y

Y

H

H

active site: T127 (≠ G110), N128 (≠ T111), Q129 (≠ P112), Y151 (≠ R136), K155 (≠ E140)
binding 2'deoxy-thymidine-5'-diphospho-alpha-d-glucose: S87 (≠ H75), H88 (≠ D76), T127 (≠ G110), N128 (≠ T111), Q129 (≠ P112), Y151 (≠ R136), N180 (≠ Q160), K190 (≠ G168), L191 (= L169), P206 (≠ A184), Y208 (≠ I186), R215 (≠ W193)
binding nicotinamide-adenine-dinucleotide: G10 (= G10), F11 (≠ N11), I12 (= I12), D37 (≠ G29), S38 (≠ L30), L39 (≠ S31), T40 (≠ R32), A42 (≠ K34), G43 (≠ E35), D63 (≠ T51), I64 (= I52), F83 (≠ L71), A84 (= A72), A85 (≠ F73), S87 (≠ H75), T102 (vs. gap), V125 (≠ T108), S126 (= S109), Y151 (≠ R136), K155 (≠ E140), N181 (≠ V161)

Sites not aligning to the query:

binding 2'deoxy-thymidine-5'-diphospho-alpha-d-glucose: 250, 274, 277, 281

1r66A Crystal structure of desiv (dtdp-glucose 4,6-dehydratase) from streptomyces venezuelae with NAD and tyd bound (see paper)
24% identity, 74% coverage: 1:219/295 of query aligns to 1:241/322 of 1r66A

query
sites
1r66A

M

M

R

R

I

L

F

L

V

V

T

T

G

G

A

G

T

A

G
|
G

N
x
F

I
|
I

G

G

S

S

N

H

V

F

V

V

R

R

E

Q

L

L

I

L

A

A

A

G

G

A

H

Y

Q

P

-

D

-

V

-

P

-

A

-

D

-

E

-

V

-

I

V

V

L

L

G
x
D

L
x
S

S
x
L

R
x
T

S

Y

K

A

E
x
G

K

N

E

R

A

A

A

N

L

L

A

A

A

P

A

V

G

D

A

A

E

D

-

P

-

R

-

L

-

R

V

F

L

V

H

H

G

G

T
x
D

I
|
I

E

R

D

D

I

A

G

G

I

L

L

L

K

A

S

R

G

E

A

L

S

R

Q

G

S

V

D

D

G

A

V

I

I

V

H

H

L
x
F

A
|
A

F
x
A

N

E

H
x
S

D
x
H

F

V

S

D

R

R

-

S

-

I

-

A

-

G

-

A

-

S

-

V

F

F

V

T

E

E

-
x
T

N

N

C

V

E

Q

D

G

D

T

R

Q

R

T

V

L

I

L

A

Q

T

C

L

A

A

V

S

D

A

A

L

G

A

V

G

G

S

R

A

-

R

-

P

-

L

V

V

V

I

H

T
x
V

S
|
S

G
x
T

T
x
D

P
x
E

I

V

A

Y

N

G

T

S

V

I

P

D

G

S

E

G

P

S

A

W

R

T

E

E

D

S

N

S

P

P

I

L

A

-

G

-

S

E

D

P

T

N

H

S

P

P

R
x
Y

A

A

A

A

S

S

E
x
K

E

A

A

G

A

S

I

D

L

L

A

V

S

A

E

R

-

A

-

Y

-

H

-

R

-

T

I

Y

G

G

V

L

N

D

V

V

S

R

V

I

V

T

R

R

L

C

P

C

Q
x
N

V
x
N

H

Y

D

G

P

P

V

Y

S

Q

-

H

-

P

Q

E

G
x
K

L
|
L

I

I

T

P

P

L

L

F

I

V

E

T

I

N

F

L

R

L

Q

D

K

G

G

G

V

T

C

L

A
x
P

Y

L

I
x
Y

E

G

D

D

G

G

L

A

N

N

R

V

W
x
R

P

E

A

W

A

V

H

H

F

T

S

D

D

D

V

H

A

C

R

R

L

G

Y

I

R

A

L

L

V

V

V

L

E

A

R

G

A

G

E

R

P

A

N

G

A

E

R

I

Y

Y

H

H

active site: T127 (≠ G110), D128 (≠ T111), E129 (≠ P112), Y151 (≠ R136), K155 (≠ E140)
binding nicotinamide-adenine-dinucleotide: G10 (= G10), F11 (≠ N11), I12 (= I12), D37 (≠ G29), S38 (≠ L30), L39 (≠ S31), T40 (≠ R32), G43 (≠ E35), D63 (≠ T51), I64 (= I52), F83 (≠ L71), A84 (= A72), A85 (≠ F73), S87 (≠ H75), T102 (vs. gap), V125 (≠ T108), S126 (= S109), Y151 (≠ R136), K155 (≠ E140), N181 (≠ V161)
binding thymidine-5'-diphosphate: H88 (≠ D76), E129 (≠ P112), N180 (≠ Q160), K190 (≠ G168), L191 (= L169), P206 (≠ A184), Y208 (≠ I186), R215 (≠ W193)

Sites not aligning to the query:

binding thymidine-5'-diphosphate: 250, 274, 277

6wj9B Udp-glcnac c4-epimerase mutant s121a/y146f from pseudomonas protegens in complex with udp-glcnac (see paper)
33% identity, 43% coverage: 2:127/295 of query aligns to 3:137/308 of 6wj9B

query
sites
6wj9B

R

R

I

I

F

L

V

V

T

T

G
|
G

A

G

T

A

G
|
G

N
x
F

I
|
I

G

G

S

S

N

H

V

L

V

V

R

D

E

A

L

L

I

L

A

A

A

K

G

G

H

Y

Q

A

V

V

L

R

-

V

-

L

-
x
D

G
x
D

L

L

S
|
S

R
x
T

S
x
G

K

K

E

V

K

G

E

N

A

L

A

P

L

M

A

G

A

D

A

A

G

G

A

L

E

E

V

L

L

L

H

V

G

G

T
x
D

I
x
A

E

A

D

D

I

A

G

A

I

L

L

L

K

A

S

D

G

A

A

V

S

Q

Q

G

S

C

D

D

G

A

V

V

I

V

H

H

L
|
L

A
|
A

F
x
A

N

V

H

A

D

S

F
x
V

S

Q

R

A

F

S

V

V

E

E

N

D

-

P

-

V

C

A

E

T

D

H

D

Q

R
x
S

R

N

V

F

I

I

A

A

T

T

L

L

-

R

-

L

-

C

-

E

A

A

S

M

A

T

L

A

A

A

G

G

S

I

A

R

R

R

P

V

L

V

V

F

I
x
A

T

S

S
x
A

G
x
A

T
x
A

P

V

I

Y

A

G

N

N

T

N

V

G

P

E

G

G

E

T

P

P

A

I

R

A

E

E

D

D

N

T

P

P

active site: A119 (≠ S109), A120 (≠ G110), A121 (≠ T111)
binding nicotinamide-adenine-dinucleotide: G8 (= G7), G11 (= G10), F12 (≠ N11), I13 (= I12), D32 (vs. gap), D33 (≠ G29), S35 (= S31), T36 (≠ R32), G37 (≠ S33), D55 (≠ T51), A56 (≠ I52), L75 (= L71), A76 (= A72), A77 (≠ F73), S94 (≠ R88), A117 (≠ I107), A119 (≠ S109)
binding uridine-diphosphate-n-acetylglucosamine: V81 (≠ F77)

Sites not aligning to the query:

active site: 144, 148
binding nicotinamide-adenine-dinucleotide: 144, 148, 171, 172, 174
binding uridine-diphosphate-n-acetylglucosamine: 173, 187, 188, 192, 203, 204, 205, 212, 248, 271, 274

6wjaA Udp-glcnac c4-epimerase mutant s121a/y146f from pseudomonas protegens in complex with udp-galnac (see paper)
33% identity, 43% coverage: 2:127/295 of query aligns to 2:136/307 of 6wjaA

query
sites
6wjaA

R

R

I

I

F

L

V

V

T

T

G
|
G

A

G

T

A

G
|
G

N
x
F

I
|
I

G

G

S

S

N

H

V

L

V

V

R

D

E

A

L

L

I

L

A

A

A

K

G

G

H

Y

Q

A

V

V

L

R

-

V

-

L

-
x
D

G
x
D

L

L

S
|
S

R
x
T

S
x
G

K

K

E

V

K

G

E

N

A

L

A

P

L

M

A

G

A

D

A

A

G

G

A

L

E

E

V

L

L

L

H

V

G

G

T

D

I
x
A

E

A

D

D

I

A

G

A

I

L

L

L

K

A

S

D

G

A

A

V

S

Q

Q

G

S

C

D

D

G

A

V

V

I

V

H

H

L
|
L

A
|
A

F
x
A

N

V

H

A

D

S

F
x
V

S

Q

R

A

F

S

V

V

E

E

N

D

-

P

-

V

C

A

E

T

D

H

D

Q

R
x
S

R

N

V

F

I

I

A

A

T

T

L

L

-

R

-

L

-

C

-

E

A

A

S

M

A

T

L

A

A

A

G

G

S

I

A

R

R

R

P

V

L

V

V

F

I

A

T

S

S
x
A

G
x
A

T
x
A

P

V

I

Y

A

G

N

N

T

N

V

G

P

E

G

G

E

T

P

P

A

I

R

A

E

E

D

D

N

T

P

P

active site: A118 (≠ S109), A119 (≠ G110), A120 (≠ T111)
binding nicotinamide-adenine-dinucleotide: G7 (= G7), G10 (= G10), F11 (≠ N11), I12 (= I12), D31 (vs. gap), D32 (≠ G29), S34 (= S31), T35 (≠ R32), G36 (≠ S33), A55 (≠ I52), L74 (= L71), A75 (= A72), A76 (≠ F73), S93 (≠ R88)
binding uridine-diphosphate-n-acetylgalactosamine: V80 (≠ F77), A120 (≠ T111)

Sites not aligning to the query:

active site: 143, 147
binding nicotinamide-adenine-dinucleotide: 143, 147, 170, 171, 173
binding uridine-diphosphate-n-acetylgalactosamine: 172, 186, 187, 191, 202, 204, 211, 247, 270, 273

Query Sequence

>WP_004128094.1 NCBI__GCF_000359745.1:WP_004128094.1
MRIFVTGATGNIGSNVVRELIAAGHQVLGLSRSKEKEAALAAAGAEVLHGTIEDIGILKS
GASQSDGVIHLAFNHDFSRFVENCEDDRRVIATLASALAGSARPLVITSGTPIANTVPGE
PAREDNPIAGSDTHPRAASEEAAILASEIGVNVSVVRLPQVHDPVSQGLITPLIEIFRQK
GVCAYIEDGLNRWPAAHFSDVARLYRLVVERAEPNARYHAVAEEGVPMRDIAEVIGRRLN
LPVKSVGKDEAQEYFGWLSMFATRDMPASSAKTRQKLGWEPTGPKLLDDLAELRV

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory