SitesBLAST

4d79A Crystal structure of e. Coli tRNA n6-threonylcarbamoyladenosine dehydratase, tcda, in complex with atp at 1.768 angstroem resolution (see paper)
48% identity, 92% coverage: 10:261/274 of query aligns to 9:239/243 of 4d79A

query
sites
4d79A

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binding adenosine-5'-triphosphate: G35 (= G36), G37 (= G38), G38 (= G39), D59 (= D60), R70 (= R71), K83 (= K84), F106 (= F107), V107 (≠ L108), D128 (= D128), P132 (≠ A132)

Q9VLJ8 Adenylyltransferase and sulfurtransferase MOCS3; Molybdenum cofactor synthesis protein 3; Ubiquitin activating enzyme 4; EC 2.7.7.80; EC 2.8.1.11 from Drosophila melanogaster (Fruit fly) (see paper)
33% identity, 51% coverage: 19:158/274 of query aligns to 81:218/453 of Q9VLJ8

query
sites
Q9VLJ8

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Sites not aligning to the query:

62 modified: Phosphothreonine

6yubB Crystal structure of uba4 from chaetomium thermophilum (see paper)
35% identity, 48% coverage: 20:150/274 of query aligns to 25:155/289 of 6yubB

query
sites
6yubB

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Sites not aligning to the query:

binding zinc ion: 184, 187, 265

6yubA Crystal structure of uba4 from chaetomium thermophilum (see paper)
35% identity, 48% coverage: 20:150/274 of query aligns to 24:154/423 of 6yubA

query
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6yubA

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Sites not aligning to the query:

binding zinc ion: 183, 186, 266, 269

8ideA Structure of an ancient tsad-tsac-sua5-tcda modular enzyme (tsan) (see paper)
31% identity, 54% coverage: 15:162/274 of query aligns to 686:835/929 of 8ideA

query
sites
8ideA

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Sites not aligning to the query:

binding manganese (ii) ion: 109, 113, 304

1jwbB Structure of the covalent acyl-adenylate form of the moeb-moad protein complex (see paper)
34% identity, 45% coverage: 27:148/274 of query aligns to 28:149/240 of 1jwbB

query
sites
1jwbB

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active site: D129 (= D128)
binding adenosine monophosphate: G37 (= G36), G39 (= G38), G40 (= G39), D61 (= D60), F62 (≠ L61), K85 (= K84), L108 (≠ F107), C127 (≠ A126), T128 (≠ I127), D129 (= D128), N130 (= N129), V133 (≠ A132)

Sites not aligning to the query:

active site: 13
binding zinc ion: 171, 236, 239

1jw9B Structure of the native moeb-moad protein complex (see paper)
34% identity, 45% coverage: 27:148/274 of query aligns to 28:149/240 of 1jw9B

query
sites
1jw9B

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active site: D129 (= D128)

Sites not aligning to the query:

active site: 13
binding zinc ion: 171, 236, 239

1jwaB Structure of the atp-bound moeb-moad protein complex (see paper)
34% identity, 45% coverage: 27:148/274 of query aligns to 28:149/217 of 1jwaB

query
sites
1jwaB

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active site: D129 (= D128)
binding adenosine-5'-triphosphate: G39 (= G38), G40 (= G39), D61 (= D60), F62 (≠ L61), R72 (= R71), K85 (= K84), L108 (≠ F107), D129 (= D128), N130 (= N129), V133 (≠ A132)

Sites not aligning to the query:

active site: 13

P12282 Molybdopterin-synthase adenylyltransferase; MoaD protein adenylase; Molybdopterin-converting factor subunit 1 adenylase; Sulfur carrier protein MoaD adenylyltransferase; EC 2.7.7.80 from Escherichia coli (strain K12) (see 2 papers)
34% identity, 45% coverage: 27:148/274 of query aligns to 29:150/249 of P12282

query
sites
P12282

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V

I

I

V

G

G

I

L

G

G

V

L

G

G

S
x
C

W

A

A

A

A

S

E

Q

A

Y

L

L

A

A

R

S

S

A

G

G

V

V

G

G

R

N

L

L

T

T

L

L

I

L

D

D

L

F

D

D

H

T

V

V

A

S

E

L

S

S

N

N

I

L

N

Q

R
|
R

Q

Q

A

T

H

L

A

H

L

S

E

D

D

A

T

T

L

V

G

G

M

Q

A

P

K

K

V

V

S

E

A

S

M

A

A

R

T

D

R

A

I

L

E

T

G

R

I

I

D

N

P

P

A

H

C

I

R

A

V

I

D

T

T

P

I

V

E

N

D

A

F

L

L

L

T

D

P

D

E

A

N

E

A

L

A

A

E

A

L

L

L

I

H

A

G

E

F

H

D

D

L

L

V

V

I

L

D

D

A
x
C

I

T

D
|
D

N

N

V

V

R

A

A

V

K

R

V

N

A

Q

I

L

A

N

V

A

T

G

C
|
C

R

F

E

A

R

A

R

K

I

V

P

P

L

L

V

V

C44 (≠ S42) mutation to A: No effect.
R73 (= R71) mutation to A: No effect. No activity; when associated with A-14.; mutation to K: Substantially reduced activity.
C128 (≠ A126) mutation to A: No effect.; mutation to Y: No activity.
D130 (= D128) mutation to A: No activity.; mutation to E: Substantially reduced activity.
C142 (= C140) mutation to A: No effect.

Sites not aligning to the query:

14 mutation R->A,K: No effect.; R→A: No activity; when associated with A-73.
172 C→A: No zinc bound and no enzyme activity.
175 C→A: No zinc bound and no enzyme activity.
187 C→A: No effect.
231 C→A: No effect.
244 C→A: No zinc bound and almost no enzyme activity.
247 C→A: No zinc bound and almost no enzyme activity.

Q06624 DNA damage tolerance protein RHC31; RAD31 homolog from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
29% identity, 50% coverage: 17:154/274 of query aligns to 21:157/347 of Q06624

query
sites
Q06624

R

R

L

L

Y

W

G

G

E

M

G

T

A

A

A

Q

A

A

R

N

L

M

A

R

G

S

A

A

H
x
K

A

V

C

L

V

L

I

I

G

N

I

L

G

G

G

A

V

I

G

G

S

S

W

E

A

I

A

T

E

K

A

S

L

I

A

V

R

L

S

S

G

G

V

I

G

G

R

H

L

L

T

T

L

I

I

L

D

D

L

G

D

H

H

M

V

V

A

T

E

E

S

E

N

D

I

L

N

G

R

S

Q

Q

A

F

H

F

A

I

L

G

E

S

D

E

T

D

L

V

G

G

M

Q

A

W

K

K

V

I

S

D

A

A

M

T

A

K

T

E

R

R

I

I

E

Q

G

D

I

L

D

N

P

P

A

R

C

I

R

E

V

L

D

N

T

F

I

D

E

K

D

Q

F

D

L

L

T

-

P

Q

E

E

N

K

A

D

A

E

E

E

L

F

H

Q

G

Q

F

F

D

D

L

L

V

V

I

V

D

A

A

T

I

E

D

M

N

Q

V

I

R

D

A

E

K

A

V

I

A

K

I

I

A

N

V

T

T

L

C

T

R

R

E

K

R

L

R

N

I

I

P

P

L

L

V

Y

M

V

A

A

G

G

G

S

A

N

G

G

K35 (≠ H31) modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)

O95396 Adenylyltransferase and sulfurtransferase MOCS3; Molybdenum cofactor synthesis protein 3; Molybdopterin synthase sulfurylase; MPT synthase sulfurylase; EC 2.7.7.80; EC 2.8.1.11 from Homo sapiens (Human) (see 5 papers)
36% identity, 46% coverage: 26:151/274 of query aligns to 79:204/460 of O95396

query
sites
O95396

R

R

L

L

A

G

G

T

A

A

H

C

A

V

C

L

V

I

I

V

G

G

I

C

G

G

V

L

G

G

S

C

W

P

A

L

A

A

E

Q

A

Y

L

L

A

A

R

A

S

A

G

G

V

V

G

G

R

R

L

L

T

G

L

L

I

V

D

D

L

Y

D

D

H

V

V

V

A

E

E

M

S

S

N

N

I

L

N

A

R

R

Q

Q

A

V

H

L

A

H

L

G

E

E

D

A

T

L

L

A

G

G

M

Q

A

A

K

K

V

A

S

F

A

S

M

A

A

A

T

A

R

S

I

L

E

R

G

R

I

L

D

N

P

S

A

A

C

V

R

E

V

C

D

V

T

P

I

Y

E
x
T

D
x
Q

F
x
A

L
|
L

T
|
T

P
|
P

E
x
A

N
x
T

A
|
A

A
x
L

E
x
D

L
|
L

L
x
V

H
x
R

G
x
R

F
x
Y

D
|
D

L
x
V

V
|
V

I
x
A

D
|
D

A
x
C

I
x
S

D
|
D

N
|
N

V
|
V

R
x
P

A
x
T

K
x
R

V
x
Y

A
x
L

I
x
V

A
x
N

V
x
D

T
x
A

C
|
C

R
x
V

E
x
L

R
x
A

R
x
G

I
x
R

P
|
P

L
|
L

V
|
V

M
x
S

A
|
A

G
x
S

Sites not aligning to the query:

158:238 Interaction with NFS1
239 C→A: Impairs sulfurtransferase activity.
316 modified: Disulfide link with 324; C→A: Does not affect sulfurtransferase activity.
324 modified: Disulfide link with 316; C→A: Does not affect sulfurtransferase activity.
365 C→A: Does not affect sulfurtransferase activity.
412 active site, Cysteine persulfide intermediate; for sulfurtransferase activity; modified: Cysteine persulfide; C→A: Abolishes sulfurtransferase activity.
413 K→R: Does not affect sulfurtransferase specificity and activity.
414 L→K: Does not affect sulfurtransferase specificity and activity.
415 G→A: Does not affect sulfurtransferase specificity and activity.
416 N→V: Does not affect sulfurtransferase specificity and activity.
417 D→R: Results in 470-fold increased activity.; D→T: Results in 90-fold increased activity.
458 P→G: Does not affect sulfurtransferase specificity and activity.
460 Y→A: Does not affect sulfurtransferase specificity and activity.

Q7SXG4 SUMO-activating enzyme subunit 2; Ubiquitin-like 1-activating enzyme E1B; Ubiquitin-like modifier-activating enzyme 2; EC 2.3.2.- from Danio rerio (Zebrafish) (Brachydanio rerio) (see paper)
29% identity, 48% coverage: 24:154/274 of query aligns to 13:144/650 of Q7SXG4

query
sites
Q7SXG4

A

A

A

D

R

S

L

L

A

S

G

S

A

C

H

R

A

V

C

L

V

V

I

V

G

G

I

A

G

G

V

I

G

G

S

C

W

E

A

L

E

K

A

N

L

L

A

V

R

L

S

T

G

G

V

F

G

K

R

N

L

I

T

E

L

V

I

I

D

D

L

L

D

D

H

T

V

I

A

D

E

V

S

S

N

N

I

L

N

N

R

R

Q

Q

A

F

H

L

A

F

L

Q

E

K

D

K

T

H

L

V

G

G

M

K

A

S

K

K

V

A

S

Q

A

V

M

A

A

K

T

E

R

S

I

V

E

L

G

R

I

F

D

C

P

P

A

S

C

A

R

N

V

I

D

T

T

A

I

Y

E

H

D

D

-

S

F

I

L

M

T

N

P

P

E

D

N

Y

A

N

A

V

E

E

L

F

H

R

G

N

F

F

D

Q

L

L

V

V

I

M

D

N

A

A

I

L

D

D

N

N

V

R

R

A

A

A

K

R

V

N

A

H

I

V

A

N

V

R

T

M

C

C

R

L

E

A

R

A

R

D

I

I

P

P

L

L

V

I

M

E

A

S

G

G

G

T

A

A

G

G

Sites not aligning to the query:

599 modified: Phosphoserine

3kydB Human sumo e1~sumo1-amp tetrahedral intermediate mimic (see paper)
30% identity, 48% coverage: 24:154/274 of query aligns to 9:140/477 of 3kydB

query
sites
3kydB

A

A

A

E

R

A

L

V

A

A

G

G

A

G

H

R

A

V

C

L

V

V

I

V

G

G

I

A

G

G

V
x
I

G

G

S

C

W

E

A

L

E

K

A

N

L

L

A

V

R

L

S

T

G

G

V

F

G

S

R

H

L

I

T

D

L

L

I

I

D
|
D

L
|
L

D

D

H

T

V

I

A

D

E

V

S

S

N

N

I

L

N

N

R

R

Q

Q

A

F

H

L

A

F

L

Q

E

K

D

K

T

H

L

V

G

G

M

R

A

S

K
|
K

V

A

S

Q

A

V

M

A

A

K

T

E

R

S

I

V

E

L

G

Q

I

F

D

Y

P

P

A

K

C

A

R

N

V

I

D

V

T

A

I

Y

E

H

D

D

-
x
S

F
x
I

L

M

T

N

P

P

E

D

N

Y

A

N

A

V

E

E

L

F

H

R

G

Q

F

F

D

I

L

L

V

V

I

M

D

N

A

A

I
x
L

D
|
D

N
|
N

V

R

R

A

A

A

K

R

V

N

A

H

I

V

A

N

V

R

T

M

C

C

R

L

E

A

R

A

R

D

I

V

P

P

L

L

V

I

M

E

A

S

G

G

G

T

A

A

G

G

active site: D114 (= D128)
binding 5'-{[(3-aminopropyl)sulfonyl]amino}-5'-deoxyadenosine: I25 (≠ V40), D45 (= D60), L46 (= L61), K69 (= K84), S92 (vs. gap), I93 (≠ F107), L113 (≠ I127), D114 (= D128), N115 (= N129)

Sites not aligning to the query:

active site: 170, 171, 173
binding 5'-{[(3-aminopropyl)sulfonyl]amino}-5'-deoxyadenosine: 170, 173
binding zinc ion: 155, 158, 370, 373

1y8qB Sumo e1 activating enzyme sae1-sae2-mg-atp complex (see paper)
30% identity, 48% coverage: 24:154/274 of query aligns to 7:138/510 of 1y8qB

query
sites
1y8qB

A

A

A

E

R

A

L

V

A

A

G

G

A

G

H

R

A

V

C

L

V

V

I

V

G

G

I

A

G

G

V

I

G

G

S

C

W

E

A

L

E

K

A

N

L

L

A

V

R

L

S

T

G

G

V

F

G

S

R

H

L

I

T

D

L

L

I

I

D
|
D

L
|
L

D

D

H

T

V

I

A

D

E

V

S

S

N

N

I

L

N

N

R

R

Q

Q

A

F

H

L

A

F

L

Q

E

K

D

K

T

H

L

V

G

G

M

R

A

S

K
|
K

V

A

S

Q

A

V

M

A

A

K

T

E

R

S

I

V

E

L

G

Q

I

F

D

Y

P

P

A

K

C

A

R

N

V

I

D

V

T

A

I

Y

E

H

D

D

-
x
S

F
x
I

L

M

T

N

P

P

E

D

N

Y

A

N

A

V

E

E

L

F

H

R

G

Q

F

F

D

I

L

L

V

V

I

M

D

N

A

A

I
x
L

D
|
D

N
|
N

V

R

R

A

A

A

K

R

V

N

A

H

I

V

A

N

V

R

T

M

C

C

R

L

E

A

R

A

R

D

I

V

P

P

L

L

V

I

M

E

A

S

G

G

G

T

A

A

G

G

active site: D112 (= D128)
binding adenosine-5'-triphosphate: D43 (= D60), L44 (= L61), K67 (= K84), S90 (vs. gap), I91 (≠ F107), L111 (≠ I127), D112 (= D128), N113 (= N129)

Sites not aligning to the query:

3kycB Human sumo e1 complex with a sumo1-amp mimic (see paper)
30% identity, 48% coverage: 24:154/274 of query aligns to 8:139/548 of 3kycB

query
sites
3kycB

A

A

A

E

R

A

L

V

A

A

G

G

A

G

H

R

A

V

C

L

V

V

I

V

G

G

I

A

G
|
G

V

I

G

G

S

C

W

E

A

L

E

K

A

N

L

L

A

V

R

L

S

T

G

G

V

F

G

S

R

H

L

I

T

D

L

L

I

I

D
|
D

L
|
L

D

D

H

T

V

I

A

D

E

V

S

S

N

N

I

L

N

N

R

R

Q

Q

A

F

H

L

A

F

L

Q

E

K

D

K

T

H

L

V

G

G

M

R

A

S

K
|
K

V

A

S

Q

A

V

M

A

A

K

T

E

R

S

I

V

E

L

G

Q

I

F

D

Y

P

P

A

K

C

A

R

N

V

I

D

V

T

A

I

Y

E

H

D

D

-
x
S

F
x
I

L

M

T

N

P

P

E

D

N

Y

A

N

A

V

E

E

L

F

H

R

G

Q

F

F

D

I

L

L

V

V

I

M

D

N

A

A

I
x
L

D
|
D

N
|
N

V

R

R

A

A

A

K

R

V

N

A

H

I

V

A

N

V

R

T

M

C

C

R

L

E

A

R

A

R

D

I

V

P

P

L

L

V

I

M

E

A

S

G

G

G

T

A

A

G

G

active site: D113 (= D128)
binding 5'-deoxy-5'-(sulfamoylamino)adenosine: G22 (= G38), G23 (= G39), D44 (= D60), L45 (= L61), K68 (= K84), S91 (vs. gap), I92 (≠ F107), L112 (≠ I127), D113 (= D128), N114 (= N129)

Sites not aligning to the query:

active site: 169, 170, 172
binding zinc ion: 154, 157, 407, 410

6xogB Structure of sumo1-ml786519 adduct bound to sae (see paper)
30% identity, 48% coverage: 24:154/274 of query aligns to 5:136/500 of 6xogB

query
sites
6xogB

A

A

A

E

R

A

L

V

A

A

G

G

A

G

H

R

A

V

C

L

V

V

I

V

G

G

I

A

G

G

V

I

G

G

S

C

W

E

A

L

E

K

A

N

L

L

A

V

R

L

S

T

G

G

V

F

G

S

R

H

L

I

T

D

L

L

I

I

D
|
D

L
|
L

D

D

H

T

V

I

A

D

E

V

S

S

N

N

I

L

N

N

R

R

Q

Q

A

F

H

L

A

F

L

Q

E

K

D

K

T

H

L

V

G

G

M

R

A

S

K
|
K

V

A

S

Q

A

V

M

A

A

K

T

E

R

S

I

V

E

L

G

Q

I

F

D

Y

P

P

A

K

C

A

R

N

V

I

D

V

T

A

I

Y

E

H

D

D

-
x
S

F
x
I

L
x
M

T

N

P

P

E

D

N

Y

A

N

A

V

E

E

L

F

H

R

G

Q

F

F

D

I

L

L

V

V

I

M

D

N

A
|
A

I

L

D
|
D

N
|
N

V

R

R

A

A

A

K

R

V

N

A

H

I

V

A

N

V

R

T

M

C

C

R

L

E

A

R

A

R

D

I

V

P

P

L

L

V

I

M

E

A

S

G

G

G

T

A

A

G

G

active site: D110 (= D128)
binding {(1R,2R,3S,4R)-4-[(5-{4-[(1S)-1-(6-bromopyridin-2-yl)-1-hydroxyethyl]thiophene-2-carbonyl}pyrimidin-4-yl)amino]-2,3-dihydroxycyclopentyl}methyl sulfamate: D41 (= D60), L42 (= L61), K65 (= K84), S88 (vs. gap), I89 (≠ F107), M90 (≠ L108), A108 (= A126), D110 (= D128), N111 (= N129)

Sites not aligning to the query:

active site: 163, 164, 166
binding zinc ion: 151, 154, 398, 401

6xohB Structure of sumo1-ml00789344 adduct bound to sae (see paper)
30% identity, 48% coverage: 24:154/274 of query aligns to 7:138/490 of 6xohB

query
sites
6xohB

A

A

A

E

R

A

L

V

A

A

G

G

A

G

H

R

A

V

C

L

V

V

I

V

G

G

I

A

G

G

V

I

G

G

S

C

W

E

A

L

E

K

A

N

L

L

A

V

R

L

S

T

G

G

V

F

G

S

R

H

L

I

T

D

L

L

I

I

D
|
D

L
|
L

D

D

H

T

V

I

A

D

E

V

S

S

N

N

I

L

N

N

R

R

Q

Q

A

F

H

L

A

F

L

Q

E

K

D

K

T

H

L

V

G

G

M

R

A

S

K
|
K

V

A

S

Q

A

V

M

A

A

K

T

E

R

S

I

V

E

L

G

Q

I

F

D

Y

P

P

A

K

C

A

R

N

V

I

D

V

T

A

I

Y

E

H

D

D

-
x
S

F
x
I

L
x
M

T

N

P

P

E

D

N

Y

A

N

A

V

E

E

L

F

H

R

G

Q

F

F

D

I

L

L

V

V

I

M

D

N

A

A

I
x
L

D
|
D

N
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N

V

R

R

A

A

A

K

R

V

N

A

H

I

V

A

N

V

R

T

M

C

C

R

L

E

A

R

A

R

D

I

V

P

P

L

L

V

I

M

E

A

S

G

G

G

T

A

A

G

G

active site: D112 (= D128)
binding {(1R,2S,4R)-4-[(5-{4-[(1R)-3,4-dihydro-1H-2-benzopyran-1-yl]thiophene-2-carbonyl}pyrimidin-4-yl)amino]-2-hydroxycyclopentyl}methyl sulfamate: D43 (= D60), L44 (= L61), K67 (= K84), S90 (vs. gap), I91 (≠ F107), M92 (≠ L108), L111 (≠ I127), D112 (= D128), N113 (= N129)

Sites not aligning to the query:

active site: 165, 166, 168
binding zinc ion: 153, 156, 391, 394

Q9UBT2 SUMO-activating enzyme subunit 2; Anthracycline-associated resistance ARX; Ubiquitin-like 1-activating enzyme E1B; Ubiquitin-like modifier-activating enzyme 2; EC 2.3.2.- from Homo sapiens (Human) (see 8 papers)
30% identity, 48% coverage: 24:154/274 of query aligns to 12:143/640 of Q9UBT2

query
sites
Q9UBT2

A

A

A

E

R

A

L

V

A

A

G

G

A

G

H

R

A

V

C

L

V

V

I

V

G
|
G

I
x
A

G
|
G

V
x
I

G
|
G

S

C

W

E

A

L

E

K

A

N

L

L

A

V

R

L

S

T

G

G

V

F

G

S

R

H

L

I

T

D

L

L

I

I

D
|
D

L

L

D

D

H

T

V

I

A

D

E

V

S

S

N
|
N

I
x
L

N
|
N

R
|
R

Q

Q

A

F

H

L

A

F

L

Q

E

K

D

K

T

H

L

V

G

G

M

R

A

S

K
|
K

V

A

S

Q

A

V

M

A

A

K

T

E

R

S

I

V

E

L

G

Q

I

F

D

Y

P

P

A

K

C

A

R

N

V

I

D

V

T

A

I

Y

E

H

D

D

-
x
S

F
x
I

L

M

T

N

P

P

E

D

N

Y

A

N

A

V

E

E

L

F

H

R

G

Q

F

F

D

I

L

L

V

V

I

M

D

N

A

A

I

L

D
|
D

N
|
N

V
x
R

R
x
A

A
|
A

K
x
R

V

N

A

H

I

V

A

N

V

R

T

M

C

C

R

L

E

A

R

A

R

D

I

V

P

P

L

L

V

I

M

E

A

S

G

G

G

T

A

A

G

G

G24 (= G36) to V: in ACCES; loss of function; does not rescue the abnormal phenotype in a zebrafish disease model; dbSNP:rs2075211884
GAGGIG 24:29 (≠ GIGGVG 36:41) binding ATP
D48 (= D60) binding ATP
N56 (= N68) mutation to A: Abolishes ATP-dependent activation of SUMO proteins.
NLNR 56:59 (≠ NINR 68:71) binding ATP
L57 (≠ I69) mutation to A: Strongly reduces ATP-dependent activation of SUMO proteins.
R59 (= R71) mutation to A: Strongly reduces ATP-dependent activation of SUMO proteins.
K72 (= K84) binding ATP; mutation to A: Abolishes ATP-dependent activation of SUMO proteins.
SI 95:96 (≠ -F 107) binding ATP
D117 (= D128) mutation to A: Abolishes ATP-dependent activation of SUMO proteins.
DNRAAR 117:122 (≠ DNVRAK 128:133) binding ATP
R122 (≠ K133) to G: in ACCES; loss of function; does not rescue the abnormal phenotype in a zebrafish disease model; dbSNP:rs1599889628

Sites not aligning to the query:

173 active site, Glycyl thioester intermediate; C→A: Loss of enzyme activity.
174 T→A: Slightly reduced enzyme activity.
184 H→Q: No effect on enzyme activity.
235 I→A: Strongly reduced interaction with UBE2I; when associated with A-238.
238 I→A: Strongly reduced interaction with UBE2I; when associated with A-235.
307 L → R: in dbSNP:rs1043062
483 E → K: in ACCES; loss of function; does not rescue the abnormal phenotype in a zebrafish disease model; dbSNP:rs2075619600
484 mutation Missing: Strongly reduced interaction with UBE2I.
485 G→GGGG: Strongly reduced interaction with UBE2I.

6xoiB Structure of sumo1-ml00752641 adduct bound to sae (see paper)
30% identity, 48% coverage: 24:154/274 of query aligns to 5:136/403 of 6xoiB

query
sites
6xoiB

A

A

A

E

R

A

L

V

A

A

G

G

A

G

H

R

A

V

C

L

V

V

I

V

G

G

I

A

G

G

G
|
G

V

I

G

G

S

C

W

E

A

L

E

K

A

N

L

L

A

V

R

L

S

T

G

G

V

F

G

S

R

H

L

I

T

D

L

L

I

I

D
|
D

L
|
L

D

D

H

T

V

I

A

D

E

V

S

S

N

N

I

L

N

N

R

R

Q

Q

A

F

H

L

A

F

L

Q

E

K

D

K

T

H

L

V

G

G

M

R

A

S

K
|
K

V

A

S

Q

A

V

M

A

A

K

T

E

R

S

I

V

E

L

G

Q

I

F

D

Y

P

P

A

K

C

A

R

N

V

I

D

V

T

A

I

Y

E

H

D

D

-
x
S

F
x
I

L

M

T

N

P

P

E

D

N

Y

A

N

A

V

E

E

L

F

H

R

G

Q

F

F

D

I

L

L

V

V

I

M

D

N

A
|
A

I
x
L

D
|
D

N
|
N

V

R

R

A

A

A

K

R

V

N

A

H

I

V

A

N

V

R

T

M

C

C

R

L

E

A

R

A

R

D

I

V

P

P

L

L

V

I

M

E

A

S

G

G

G

T

A

A

G

G

active site: D110 (= D128)
binding [(1R,2R,3S,4R)-4-{[5-(1-benzyl-1H-pyrazole-3-carbonyl)pyrimidin-4-yl]amino}-2,3-dihydroxycyclopentyl]methyl sulfamate: G20 (= G39), D41 (= D60), L42 (= L61), K65 (= K84), S88 (vs. gap), I89 (≠ F107), A108 (= A126), L109 (≠ I127), D110 (= D128), N111 (= N129)

Sites not aligning to the query:

active site: 159, 160, 162
binding zinc ion: 151, 154, 353, 356

Query Sequence

>WP_004298477.1 NCBI__GCF_000310185.1:WP_004298477.1
MTSATTDRARRFGGIDRLYGEGAAARLAGAHACVIGIGGVGSWAAEALARSGVGRLTLID
LDHVAESNINRQAHALEDTLGMAKVSAMATRIEGIDPACRVDTIEDFLTPENAAELLHGF
DLVIDAIDNVRAKVAIAVTCRERRIPLVMAGGAGGKRDPARIGMDDLARTLQDPLLAKVR
ARLRKEHGFPRDPKKKFGIEAVFSTEPLHFPAVDACDAAHPGAASAPQGLACAGYGSSMA
VTASVGLLCAARALEHLLRQADAPARDATPEPSA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory