SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_004315099.1 NCBI__GCF_000310185.1:WP_004315099.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 5 hits to proteins with known functional sites (download)

3tdtA Complex of tetrahydrodipicolinate n-succinyltransferase with 2-amino- 6-oxopimelate and coenzyme a (see paper)
70% identity, 99% coverage: 1:271/273 of query aligns to 1:271/274 of 3tdtA

query
sites
3tdtA

M

M

H

Q

A

Q

L

L

Q

Q

S

N

I

V

I

I

D

E

T

S

A

A

F

F

E

E

N

R

R

R

A

A

S

D

L

I

S

T

P

P

T

A

S

N

A

V

P

D

A

T

E

V

I

T

R

R

N

E

A

A

V

V

E

N

E

Q

V

V

I

I

A

G

G

L

L

L

D

D

A

S

G

G

T

A

L

L

R

R

V

V

A

A

E

E

K

K

K

I

D

D

G

G

Q

Q

W

W

V

V

V

T

N

H

Q

Q

W

W

I

L

K

K

K

K

A

A

V

V

L

L

I

L

S

S

F

F

R

R

L

I

R

N

D

D

N

N

E

K

L

V

V

M

Q

D

A

G

G

A

G

E

L

T

N

R

F

Y

F

Y

D

D

K

K

V

V

A

P

T

M

K

K

F

F

G

A

D

D

Y

Y

T

D

P

E

E

A

Q

R

F

F

R

Q

E

K

G

E

G

G

F

F

R
|
R

V

V

V

V

P

P

P

P

A

A

V

T

A

V

R

R

K

Q

G

G

S

A

F

F

I

I

G

A

R

R

N

N

V

T

V

V

L

L

M
|
M

P

P

S

S

Y

Y

V

V

N

N

I

I

G

G

A

A

F

Y

V

V

D

D

E

E

G

G

T

T

M
|
M

V

V

D
|
D

T

T

W

W

A

A

T

T

V

V

G

G

S

S

C

C

A

A

Q

Q

I

I

G

G

K

K

N

N

V

V

H

H

L

L

S

S

G

G

G

G

V

V

G

G

I

I

G

G

G
|
G

V

V

L

L

E

E

P

P

V

L

Q

Q

A

A

G

N

P

P

V

T

I

I

I

I

E

E

D

D

N

N

V

C

F

F

V

I

G
|
G

A
|
A

R

R

S

S

E
|
E

V

V

V

V

E

E

G

G

V

V

I

I

I

V

E

E

E

E

N

G

A

S

V

V

L

I

S
|
S

M
|
M

G

G

V

V

Y

Y

I

L

G

G

M

Q

S

S

T

T

K

R

I

I

Y

Y

D

D

R
|
R

E

E

T

T

G

G

E

E

I

I

T

H

Y

Y

G

G

R

R

V

V

P

P

A

A

G

G

A

S

V

V

V

V

V
|
V

P

S

G

G

S

N

L

L

P

P

S

S

A

K

C

D

G

G

K

S

Y

Y

S

S

L

L

Y

Y

C

C

A

A

V

V

I

I

V

V

K
|
K

K

K

V

V

D

D

A

A

Q
x
K

T
|
T

R

R

A

G

K
|
K

T

V

G

G

I

I

N

N

E

E

L

L

L

L

R

R

active site: D141 (= D141), G166 (= G166), E189 (= E189)
binding 2-amino-6-oxopimelic acid: R104 (= R104), M124 (= M124), M139 (= M139), D141 (= D141)
binding coenzyme a: G185 (= G185), A186 (= A186), S203 (= S203), M204 (= M204), R217 (= R217), V234 (= V234), K254 (= K254), K259 (≠ Q259), T260 (= T260), K263 (= K263)

2tdtA Complex of tetrahydrodipicolinate n-succinyltransferase with 2- aminopimelate and coenzyme a (see paper)
70% identity, 99% coverage: 1:271/273 of query aligns to 1:271/274 of 2tdtA

query
sites
2tdtA

M

M

H

Q

A

Q

L

L

Q

Q

S

N

I

V

I

I

D

E

T

S

A

A

F

F

E

E

N

R

R

R

A

A

S

D

L

I

S

T

P

P

T

A

S

N

A

V

P

D

A

T

E

V

I

T

R

R

N

E

A

A

V

V

E

N

E

Q

V

V

I

I

A

G

G

L

L

L

D

D

A

S

G

G

T

A

L

L

R

R

V

V

A

A

E

E

K

K

K

I

D

D

G

G

Q

Q

W

W

V

V

V

T

N

H

Q

Q

W

W

I

L

K

K

K

K

A

A

V

V

L

L

I

L

S

S

F

F

R

R

L

I

R

N

D

D

N

N

E

K

L

V

V

M

Q

D

A

G

G

A

G

E

L

T

N

R

F

Y

F

Y

D

D

K

K

V

V

A

P

T

M

K

K

F

F

G

A

D

D

Y

Y

T

D

P

E

E

A

Q

R

F

F

R

Q

E

K

G

E

G

G

F

F

R
|
R

V

V

V

V

P

P

P

P

A

A

V

T

A

V

R

R

K

Q

G

G

S

A

F

F

I

I

G

A

R

R

N

N

V

T

V

V

L

L

M
|
M

P

P

S

S

Y

Y

V

V

N

N

I

I

G

G

A

A

F

Y

V

V

D

D

E

E

G

G

T

T

M
|
M

V

V

D
|
D

T

T

W

W

A

A

T

T

V

V

G

G

S

S

C

C

A

A

Q

Q

I

I

G

G

K

K

N

N

V

V

H

H

L

L

S
|
S

G

G

G

G

V

V

G

G

I

I

G

G

G
|
G

V

V

L

L

E

E

P

P

V

L

Q

Q

A

A

G

N

P

P

V

T

I

I

I

I

E

E

D

D

N

N

V

C

F
|
F

V

I

G
|
G

A

A

R

R

S

S

E
|
E

V

V

V

V

E

E

G

G

V

V

I

I

I

V

E

E

E

E

N

G

A

S

V

V

L

I

S
|
S

M
|
M

G

G

V

V

Y

Y

I

L

G

G

M

Q

S

S

T

T

K

R

I

I

Y

Y

D

D

R
|
R

E

E

T

T

G

G

E

E

I

I

T

H

Y

Y

G

G

R

R

V

V

P

P

A

A

G

G

A

S

V

V

V

V

V

V

P

S

G

G

S

N

L

L

P

P

S

S

A

K

C

D

G

G

K

S

Y

Y

S

S

L

L

Y

Y

C

C

A

A

V

V

I

I

V

V

K
|
K

K

K

V

V

D

D

A

A

Q
x
K

T
|
T

R

R

A

G

K
|
K

T

V

G

G

I

I

N

N

E

E

L

L

L

L

R

R

active site: D141 (= D141), G166 (= G166), E189 (= E189)
binding coenzyme a: S159 (= S159), F183 (= F183), G185 (= G185), S203 (= S203), M204 (= M204), R217 (= R217), K254 (= K254), K259 (≠ Q259), T260 (= T260), K263 (= K263)
binding (2S)-2-aminoheptanedioic acid: R104 (= R104), M124 (= M124), M139 (= M139), D141 (= D141)

1kgtA Crystal structure of tetrahydrodipicolinate n-succinyltransferase in complex with pimelate and succinyl-coa (see paper)
70% identity, 99% coverage: 1:271/273 of query aligns to 1:271/274 of 1kgtA

query
sites
1kgtA

M

M

H

Q

A

Q

L

L

Q

Q

S

N

I

V

I

I

D

E

T

S

A

A

F

F

E

E

N

R

R

R

A

A

S

D

L

I

S

T

P

P

T

A

S

N

A

V

P

D

A

T

E

V

I

T

R

R

N

E

A

A

V

V

E

N

E

Q

V

V

I

I

A

G

G

L

L

L

D

D

A

S

G

G

T

A

L

L

R

R

V

V

A

A

E

E

K

K

K

I

D

D

G

G

Q

Q

W

W

V

V

V

T

N

H

Q

Q

W

W

I

L

K

K

K

K

A

A

V

V

L

L

I

L

S

S

F

F

R

R

L

I

R

N

D

D

N

N

E

K

L

V

V

M

Q

D

A

G

G

A

G

E

L

T

N

R

F

Y

F

Y

D

D

K

K

V

V

A

P

T

M

K

K

F

F

G

A

D

D

Y

Y

T

D

P

E

E

A

Q

R

F

F

R

Q

E

K

G

E

G

G

F

F

R
|
R

V

V

V

V

P

P

P

P

A

A

V

T

A

V

R

R

K

Q

G

G

S

A

F

F

I

I

G

A

R

R

N

N

V

T

V

V

L

L

M
|
M

P

P

S

S

Y

Y

V

V

N

N

I

I

G

G

A

A

F

Y

V

V

D

D

E

E

G

G

T

T

M
|
M

V

V

D
|
D

T

T

W

W

A

A

T

T

V

V

G

G

S

S

C

C

A

A

Q

Q

I

I

G

G

K

K

N

N

V

V

H

H

L

L

S
|
S

G

G

G

G

V

V

G

G

I

I

G

G

G
|
G

V

V

L

L

E

E

P

P

V

L

Q

Q

A

A

G

N

P

P

V

T

I

I

I

I

E

E

D

D

N

N

V

C

F

F

V

I

G

G

A
|
A

R

R

S

S

E
|
E

V

V

V

V

E

E

G

G

V

V

I

I

I

V

E

E

E

E

N

G

A

S

V

V

L

I

S
|
S

M

M

G

G

V

V

Y

Y

I

L

G

G

M

Q

S

S

T

T

K

R

I

I

Y

Y

D

D

R
|
R

E

E

T

T

G

G

E

E

I

I

T

H

Y

Y

G

G

R

R

V

V

P

P

A

A

G

G

A

S

V

V

V

V

V

V

P

S

G

G

S

N

L

L

P

P

S

S

A

K

C

D

G

G

K

S

Y

Y

S

S

L

L

Y

Y

C

C

A

A

V

V

I

I

V

V

K

K

K

K

V

V

D

D

A

A

Q
x
K

T
|
T

R

R

A

G

K
|
K

T

V

G

G

I

I

N

N

E

E

L

L

L

L

R

R

active site: D141 (= D141), G166 (= G166), E189 (= E189)
binding pimelic acid: R104 (= R104), M124 (= M124), M139 (= M139)
binding succinyl-coenzyme a: D141 (= D141), S159 (= S159), A186 (= A186), S203 (= S203), R217 (= R217), K259 (≠ Q259), T260 (= T260), K263 (= K263)

1kgqA Crystal structure of tetrahydrodipicolinate n-succinyltransferase in complex with l-2-aminopimelate and succinamide-coa (see paper)
70% identity, 99% coverage: 1:271/273 of query aligns to 1:271/274 of 1kgqA

query
sites
1kgqA

M

M

H

Q

A

Q

L

L

Q

Q

S

N

I

V

I

I

D

E

T

S

A

A

F

F

E

E

N

R

R

R

A

A

S

D

L

I

S

T

P

P

T

A

S

N

A

V

P

D

A

T

E

V

I

T

R

R

N

E

A

A

V

V

E

N

E

Q

V

V

I

I

A

G

G

L

L

L

D

D

A

S

G

G

T

A

L

L

R

R

V

V

A

A

E

E

K

K

K

I

D

D

G

G

Q

Q

W

W

V

V

V

T

N

H

Q

Q

W

W

I

L

K

K

K

K

A

A

V

V

L

L

I

L

S

S

F

F

R

R

L

I

R

N

D

D

N

N

E

K

L

V

V

M

Q

D

A

G

G

A

G

E

L

T

N

R

F

Y

F

Y

D

D

K

K

V

V

A

P

T

M

K

K

F

F

G

A

D

D

Y

Y

T

D

P

E

E

A

Q

R

F

F

R

Q

E

K

G

E

G

G

F

F

R
|
R

V

V

V

V

P

P

P

P

A

A

V

T

A

V

R

R

K

Q

G

G

S

A

F

F

I

I

G

A

R

R

N

N

V

T

V

V

L

L

M
|
M

P

P

S

S

Y

Y

V

V

N

N

I

I

G

G

A

A

F

Y

V

V

D

D

E

E

G

G

T

T

M
|
M

V

V

D
|
D

T

T

W

W

A

A

T

T

V

V

G

G

S

S

C

C

A

A

Q

Q

I

I

G

G

K

K

N

N

V

V

H

H

L

L

S

S

G

G

G

G

V

V

G

G

I

I

G

G

G
|
G

V

V

L

L

E

E

P

P

V

L

Q

Q

A

A

G

N

P

P

V

T

I

I

I

I

E

E

D

D

N

N

V

C

F
|
F

V

I

G
|
G

A

A

R

R

S

S

E
|
E

V

V

V

V

E

E

G

G

V

V

I

I

I

V

E

E

E

E

N

G

A

S

V

V

L

I

S
|
S

M
|
M

G

G

V

V

Y

Y

I

L

G

G

M

Q

S

S

T

T

K

R

I

I

Y

Y

D

D

R
|
R

E

E

T

T

G

G

E

E

I

I

T

H

Y

Y

G

G

R

R

V

V

P

P

A

A

G

G

A

S

V

V

V

V

V
|
V

P

S

G

G

S

N

L

L

P

P

S

S

A

K

C

D

G

G

K

S

Y

Y

S

S

L

L

Y

Y

C

C

A

A

V

V

I

I

V

V

K
|
K

K

K

V

V

D

D

A

A

Q
x
K

T
|
T

R

R

A

G

K
|
K

T

V

G

G

I

I

N

N

E

E

L

L

L

L

R

R

active site: D141 (= D141), G166 (= G166), E189 (= E189)
binding (2S)-2-aminoheptanedioic acid: R104 (= R104), M124 (= M124), M139 (= M139), D141 (= D141)
binding succinamide-coa: F183 (= F183), G185 (= G185), S203 (= S203), M204 (= M204), R217 (= R217), V234 (= V234), K254 (= K254), K259 (≠ Q259), T260 (= T260), K263 (= K263)

3r8yA Structure of the bacillus anthracis tetrahydropicolinate succinyltransferase
46% identity, 33% coverage: 104:194/273 of query aligns to 77:168/203 of 3r8yA

query
sites
3r8yA

R

R

V

I

V

E

P

P

P

G

A

A

V

I

A

I

R

R

K

D

G

H

S

V

F

E

I

I

G

G

R

D

N

N

-

A

V

V

V

I

L

M

M

M

P

N

S

A

Y

T

V

I

N

N

I

I

G

G

A

A

F

V

V

I

D

G

E

E

G

G

T

S

M

M

V

I

D
|
D

T

M

W

N

A

A

T

V

V

L

G
|
G

S
x
G

C

R

A

A

Q

T

I

V

G

G

K

K

N

N

V

C

H

H

L

V

S

G

G

A

G

G

V

A

G

V

I

L

G

A

G
|
G

V

V

L

I

E

E

P

P

V

P

Q

S

A

A

G

K

P

P

V

V

I

I

I

V

E

E

D

D

N

D

V

V

F

V

V

I

G

G

A

A

R

N

S

V

E
x
V

V

V

V

L

E

E

G

G

V

V

active site: D115 (= D141), G140 (= G166), V163 (≠ E189)
binding calcium ion: G121 (= G147), G122 (≠ S148)

Query Sequence

>WP_004315099.1 NCBI__GCF_000310185.1:WP_004315099.1
MHALQSIIDTAFENRASLSPTSAPAEIRNAVEEVIAGLDAGTLRVAEKKDGQWVVNQWIK
KAVLISFRLRDNELVQAGGLNFFDKVATKFGDYTPEQFREGGFRVVPPAVARKGSFIGRN
VVLMPSYVNIGAFVDEGTMVDTWATVGSCAQIGKNVHLSGGVGIGGVLEPVQAGPVIIED
NVFVGARSEVVEGVIIEENAVLSMGVYIGMSTKIYDRETGEITYGRVPAGAVVVPGSLPS
ACGKYSLYCAVIVKKVDAQTRAKTGINELLRGA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory