SitesBLAST

O00116 Alkyldihydroxyacetonephosphate synthase, peroxisomal; Alkyl-DHAP synthase; Aging-associated gene 5 protein; Alkylglycerone-phosphate synthase; EC 2.5.1.26 from Homo sapiens (Human) (see 2 papers)
27% identity, 52% coverage: 14:171/301 of query aligns to 222:385/658 of O00116

query
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O00116

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Sites not aligning to the query:

182 R → Q: in RCDP3; severely reduced protein levels
419 Important for enzyme activity; R → H: in RCDP3; loss of enzyme activity; dbSNP:rs121434411
471 E → K: in RCDP3; severely reduced protein levels
568 T → M: in RCDP3; does not affect protein levels; dbSNP:rs387907214

4bc7B Mammalian alkyldihydroxyacetonephosphate synthase: arg419his mutant (see paper)
27% identity, 52% coverage: 14:171/301 of query aligns to 128:291/543 of 4bc7B

query
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4bc7B

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binding flavin-adenine dinucleotide: P140 (vs. gap), G142 (= G26), G143 (= G27), G144 (= G28), T145 (≠ S29), S146 (≠ K30), V147 (≠ D31), G150 (= G34), L151 (≠ C35), P208 (= P86), D209 (≠ P87), S210 (≠ H88), S214 (≠ A93), T215 (= T94), G218 (= G97), S221 (≠ T100), T222 (≠ A101), A224 (≠ L103), S225 (= S104), E274 (≠ L154), G275 (= G155), I280 (≠ L160)

Sites not aligning to the query:

5ae3A Ether lipid-generating enzyme agps in complex with antimycin a (see paper)
27% identity, 52% coverage: 14:171/301 of query aligns to 128:291/539 of 5ae3A

query
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5ae3A

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binding flavin-adenine dinucleotide: P140 (vs. gap), I141 (vs. gap), G142 (= G26), G143 (= G27), G144 (= G28), T145 (≠ S29), S146 (≠ K30), V147 (≠ D31), L151 (≠ C35), P208 (= P86), D209 (≠ P87), S210 (≠ H88), S214 (≠ A93), T215 (= T94), G218 (= G97), W219 (≠ C98), S221 (≠ T100), T222 (≠ A101), A224 (≠ L103), S225 (= S104), E274 (≠ L154), V279 (≠ I159), I280 (≠ L160)

Sites not aligning to the query:

5ae1B Ether lipid-generating enzyme agps in complex with inhibitor zinc69435460 (see paper)
27% identity, 52% coverage: 14:171/301 of query aligns to 128:291/539 of 5ae1B

query
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5ae1B

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binding flavin-adenine dinucleotide: P140 (vs. gap), I141 (vs. gap), G142 (= G26), G143 (= G27), G144 (= G28), T145 (≠ S29), S146 (≠ K30), G150 (= G34), L151 (≠ C35), P208 (= P86), D209 (≠ P87), S210 (≠ H88), S214 (≠ A93), T215 (= T94), G218 (= G97), W219 (≠ C98), S221 (≠ T100), T222 (≠ A101), A224 (≠ L103), S225 (= S104), E274 (≠ L154), V279 (≠ I159), I280 (≠ L160)

Sites not aligning to the query:

6gouA Development of alkyl glycerone phosphate synthase inhibitors: complex with inhibitor 2i (see paper)
27% identity, 52% coverage: 14:171/301 of query aligns to 142:305/554 of 6gouA

query
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6gouA

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binding (3~{S})-3-[2,6-bis(fluoranyl)phenyl]-~{N}-[(2-oxidanylidene-1,3-dihydrobenzimidazol-5-yl)methyl]butanamide: D223 (≠ P87)
binding flavin-adenine dinucleotide: P154 (vs. gap), I155 (vs. gap), G156 (= G26), G157 (= G27), G158 (= G28), T159 (≠ S29), S160 (≠ K30), V161 (≠ D31), G164 (= G34), P222 (= P86), D223 (≠ P87), S224 (≠ H88), S228 (≠ A93), T229 (= T94), G232 (= G97), W233 (≠ C98), S235 (≠ T100), T236 (≠ A101), A238 (≠ L103), S239 (= S104), E288 (≠ L154), V293 (≠ I159), I294 (≠ L160)

Sites not aligning to the query:

5adzC Ether lipid-generating enzyme agps in complex with inhibitor 1a (see paper)
27% identity, 52% coverage: 14:171/301 of query aligns to 142:305/557 of 5adzC

query
sites
5adzC

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binding flavin-adenine dinucleotide: P154 (vs. gap), I155 (vs. gap), G156 (= G26), G157 (= G27), G158 (= G28), T159 (≠ S29), S160 (≠ K30), P222 (= P86), D223 (≠ P87), S224 (≠ H88), S228 (≠ A93), T229 (= T94), G232 (= G97), W233 (≠ C98), S235 (≠ T100), T236 (≠ A101), A238 (≠ L103), S239 (= S104), E288 (≠ L154), G289 (= G155), I294 (≠ L160)
binding (3S)-3-(2-fluorophenyl)-N-((2-oxo-2,3-dihydro-1H-benzo[d]imidazol-5-yl)methyl)butanamide): D223 (≠ P87)

Sites not aligning to the query:

4bc9A Mammalian alkyldihydroxyacetonephosphate synthase: wild-type, adduct with cyanoethyl (see paper)
27% identity, 52% coverage: 14:171/301 of query aligns to 133:296/555 of 4bc9A

query
sites
4bc9A

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Q

L

L

A

K

D

E

K

S

G

G

Q

Y

Y

C

L

T

A

G

F

H

E

E

P
|
P

P
x
D

H
x
S

F

L

G

-

P

E

G

F

A
x
S

T
|
T

V

V

G

G

G
|
G

C
x
W

V

I

T
x
S

A
x
T

G

R

L
x
A

S
|
S

G

G

P

M

R

K

A

N

S

I

A

Y

G

G

A

N

V

I

R

E

D

D

F

L

M

V

L

V

G

H

V

M

K

K

L

V

D

T

G

P

R

R

G

G

E

-

V

V

L

I

R

E

F

K

G

S

G

C

Q

Q

V

G

M

P

K

R

N

M

V

S

A

T

G

G

Y

P

D

D

V

I

S

H

R

H

L

F

V

I

A

M

G

G

S

S

L
x
E

G

G

T

T

L

L

G

G

I
x
V

L
x
I

L

T

E

E

V

A

S

T

L

I

K

K

V

I

L

R

P

P

R

T

P

P

binding propanenitrile: D214 (≠ P87)
binding flavin-adenine dinucleotide: P145 (vs. gap), I146 (vs. gap), G147 (= G26), G148 (= G27), G149 (= G28), T150 (≠ S29), S151 (≠ K30), V152 (≠ D31), G155 (= G34), L156 (≠ C35), P213 (= P86), D214 (≠ P87), S215 (≠ H88), S219 (≠ A93), T220 (= T94), G223 (= G97), W224 (≠ C98), S226 (≠ T100), T227 (≠ A101), A229 (≠ L103), S230 (= S104), E279 (≠ L154), V284 (≠ I159), I285 (≠ L160)

Sites not aligning to the query:

5ae2B Ether lipid-generating enzyme agps in complex with inhibitor 1e (see paper)
27% identity, 52% coverage: 14:171/301 of query aligns to 128:291/542 of 5ae2B

query
sites
5ae2B

A

V

S

N

V

L

A

A

T

C

R

K

T

Y

P

N

L

L

C

C

L

I

R

I

-
x
P

-

I

G
|
G

S
x
T

K
x
S

D

V

F

S

F

Y

G
|
G

C
x
L

T

M

L

C

A

P

G

A

E

D

R

E

-

T

-

R

-

T

-

I

-

S

F

L

D

D

L

T

R

S

A

Q

N

M

R

N

G

R

V

I

V

L

A

W

Y

V

E

D

P

E

T

N

E

N

L

L

V

T

V

A

T

H

V

V

C

E

A

A

G

G

T

I

P

T

L

G

A

Q

E

E

L

L

E

E

A

R

V

Q

L

L

A

K

D

E

K

S

G

G

Q

Y

Y

C

L

T

A

G

F

H

E

E

P
|
P

P
x
D

H
x
S

F

L

G

-

P

E

G

F

A
x
S

T
|
T

V

V

G

G

G
|
G

C
x
W

V

I

T
x
S

A
x
T

G

R

L
x
A

S
|
S

G

G

P

M

R

K

A

N

S

I

A

Y

G

G

A

N

V

I

R

E

D

D

F

L

M

V

L

V

G

H

V

M

K

K

L

V

D

T

G

P

R

R

G

G

E

-

V

V

L

I

R

E

F

K

G

S

G

C

Q

Q

V

G

M

P

K

R

N

M

V

S

A

T

G

G

Y

P

D

D

V

I

S

H

R

H

L

F

V

I

A

M

G

G

S

S

L
x
E

G
|
G

T

T

L

L

G

G

I
x
V

L
x
I

L

T

E

E

V

A

S

T

L

I

K

K

V

I

L

R

P

P

R

T

P

P

binding flavin-adenine dinucleotide: P140 (vs. gap), G142 (= G26), G143 (= G27), G144 (= G28), T145 (≠ S29), S146 (≠ K30), G150 (= G34), L151 (≠ C35), P208 (= P86), D209 (≠ P87), S210 (≠ H88), S214 (≠ A93), T215 (= T94), G218 (= G97), W219 (≠ C98), S221 (≠ T100), T222 (≠ A101), A224 (≠ L103), S225 (= S104), E274 (≠ L154), G275 (= G155), V279 (≠ I159), I280 (≠ L160)

Sites not aligning to the query:

4bc9B Mammalian alkyldihydroxyacetonephosphate synthase: wild-type, adduct with cyanoethyl (see paper)
27% identity, 52% coverage: 14:171/301 of query aligns to 128:291/542 of 4bc9B

query
sites
4bc9B

A

V

S

N

V

L

A

A

T

C

R

K

T

Y

P

N

L

L

C

C

L

I

R

I

-
x
P

-

I

G
|
G

S
x
T

K
x
S

D
x
V

F

S

F

Y

G
|
G

C
x
L

T

M

L

C

A

P

G

A

E

D

R

E

-

T

-

R

-

T

-

I

-

S

F

L

D

D

L

T

R

S

A

Q

N

M

R

N

G

R

V

I

V

L

A

W

Y

V

E

D

P

E

T

N

E

N

L

L

V

T

V

A

T

H

V

V

C

E

A

A

G

G

T

I

P

T

L

G

A

Q

E

E

L

L

E

E

A

R

V

Q

L

L

A

K

D

E

K

S

G

G

Q

Y

Y

C

L

T

A

G

F

H

E

E

P
|
P

P
x
D

H
x
S

F

L

G

-

P

E

G

F

A
x
S

T
|
T

V

V

G

G

G
|
G

C
x
W

V

I

T
x
S

A
x
T

G

R

L
x
A

S
|
S

G

G

P

M

R

K

A

N

S

I

A

Y

G

G

A

N

V

I

R

E

D

D

F

L

M

V

L

V

G

H

V

M

K

K

L

V

D

T

G

P

R

R

G

G

E

-

V

V

L

I

R

E

F

K

G

S

G

C

Q

Q

V

G

M

P

K

R

N

M

V

S

A

T

G

G

Y

P

D

D

V

I

S

H

R

H

L

F

V

I

A

M

G

G

S

S

L
x
E

G
|
G

T

T

L

L

G

G

I

V

L
x
I

L

T

E

E

V

A

S

T

L

I

K

K

V

I

L

R

P

P

R

T

P

P

binding propanenitrile: D209 (≠ P87)
binding flavin-adenine dinucleotide: P140 (vs. gap), G142 (= G26), G143 (= G27), G144 (= G28), T145 (≠ S29), S146 (≠ K30), V147 (≠ D31), G150 (= G34), L151 (≠ C35), P208 (= P86), D209 (≠ P87), S210 (≠ H88), S214 (≠ A93), T215 (= T94), G218 (= G97), W219 (≠ C98), S221 (≠ T100), T222 (≠ A101), A224 (≠ L103), S225 (= S104), E274 (≠ L154), G275 (= G155), I280 (≠ L160)

Sites not aligning to the query:

P97275 Alkyldihydroxyacetonephosphate synthase, peroxisomal; Alkyl-DHAP synthase; Alkylglycerone-phosphate synthase; EC 2.5.1.26 from Cavia porcellus (Guinea pig) (see 4 papers)
27% identity, 52% coverage: 15:171/301 of query aligns to 223:385/658 of P97275

query
sites
P97275

S

N

V

L

A

A

T

C

R

K

T

Y

P

N

L

L

C

C

L

I

R

I

-
x
P

-
x
I

G
|
G

S
x
T

K
x
S

D

V

F

S

F

Y

G

G

C

L

T

M

L

C

A

P

G

A

E

D

R

E

-

T

-

R

-

T

-

I

-

S

F

L

D

D

L

T

R

S

A

Q

N

M

R

N

G

R

V

I

V

L

A

W

Y

V

E

D

P

E

T

N

E

N

L

L

V

T

V

A

T

H

V

V

C

E

A

A

G

G

T

I

P

T

L

G

A

Q

E

E

L

L

E

E

A

R

V

Q

L

L

A

K

D

E

K

S

G

G

Q

Y

Y

C

L

T

A

G

F
x
H

E

E

P

P

P
x
D

H
x
S

F
x
L

G

-

P
x
E

G
x
F

A
x
S

T
|
T

V

V

G

G

C

W

V

I

T

S

A
x
T

G
x
R

L
x
A

S
|
S

G

G

P

M

R

K

A

N

S

I

A

Y

G

G

A

N

V

I

R

E

D

D

F

L

M

V

L

V

G

H

V

M

K

K

L

V

D

T

G

P

R

R

G

G

E

-

V

V

L

I

R

E

F

K

G

S

G

C

Q

Q

V

G

M

P

K

R

N

M

V

S

A

T

G

G

Y

P

D

D

V

I

S

H

R

H

L

F

V

I

A

M

G

G

S
|
S

L
x
E

G
|
G

T
|
T

L
|
L

G
|
G

I
x
V

L
x
I

L

T

E

E

V

A

S

T

L

I

K

K

V

I

L

R

P

P

R

T

P

P

234:240 (vs. 26:30, 43% identical) binding FAD
H300 (≠ F84) mutation to A: Loss of activity.
303:309 (vs. 87:94, 13% identical) binding FAD
T309 (= T94) mutation to I: Impaired FAD binding and protein stability. Loss of activity.
TRAS 316:319 (≠ AGLS 101:104) binding FAD
S367 (= S153) mutation to A: Strongly reduced activity.
368:374 (vs. 154:160, 57% identical) binding FAD

Sites not aligning to the query:

1:58 modified: transit peptide, Peroxisome
419 Important for enzyme activity; R→H: Loss of activity.; R→K: Strongly reduced activity.
469 L→P: Impaired FAD binding and protein stability. Loss of activity.
515 binding substrate; R→L: Impaired FAD binding and protein stability. Loss of activity.
576 C→A: No effect on activity.
578 active site, Proton donor/acceptor; Y→F: Loss of activity.
615 H→A: Loss of activity.
615:617 Important for enzyme activity
616 H→A: Loss of activity.
617 H→A: Loss of activity.
654:658 Important for enzyme activity

5ae2A Ether lipid-generating enzyme agps in complex with inhibitor 1e (see paper)
27% identity, 52% coverage: 14:171/301 of query aligns to 133:296/561 of 5ae2A

query
sites
5ae2A

A

V

S

N

V

L

A

A

T

C

R

K

T

Y

P

N

L

L

C

C

L

I

R

I

-
x
P

-
x
I

G
|
G

S
x
T

K
x
S

D

V

F

S

F

Y

G
|
G

C
x
L

T

M

L

C

A

P

G

A

E

D

R

E

-

T

-

R

-

T

-

I

-

S

F

L

D

D

L

T

R

S

A

Q

N

M

R

N

G

R

V

I

V

L

A

W

Y

V

E

D

P

E

T

N

E

N

L

L

V

T

V

A

T

H

V

V

C

E

A

A

G

G

T

I

P

T

L

G

A

Q

E

E

L

L

E

E

A

R

V

Q

L

L

A

K

D

E

K

S

G

G

Q

Y

Y

C

L

T

A

G

F

H

E

E

P
|
P

P
x
D

H
x
S

F

L

G

-

P

E

G

F

A
x
S

T
|
T

V

V

G

G

G
|
G

C
x
W

V

I

T
x
S

A
x
T

G

R

L
x
A

S
|
S

G

G

P

M

R

K

A

N

S

I

A

Y

G

G

A

N

V

I

R

E

D

D

F

L

M

V

L

V

G

H

V

M

K

K

L

V

D

T

G

P

R

R

G

G

E

-

V

V

L

I

R

E

F

K

G

S

G

C

Q

Q

V

G

M

P

K

R

N

M

V

S

A

T

G

G

Y

P

D

D

V

I

S

H

R

H

L

F

V

I

A

M

G

G

S

S

L
x
E

G

G

T

T

L

L

G

G

I
x
V

L
x
I

L

T

E

E

V

A

S

T

L

I

K

K

V

I

L

R

P

P

R

T

P

P

binding flavin-adenine dinucleotide: P145 (vs. gap), I146 (vs. gap), G147 (= G26), G148 (= G27), G149 (= G28), T150 (≠ S29), S151 (≠ K30), G155 (= G34), L156 (≠ C35), P213 (= P86), D214 (≠ P87), S215 (≠ H88), S219 (≠ A93), T220 (= T94), G223 (= G97), W224 (≠ C98), S226 (≠ T100), T227 (≠ A101), A229 (≠ L103), S230 (= S104), E279 (≠ L154), V284 (≠ I159), I285 (≠ L160)

Sites not aligning to the query:

5ae1A Ether lipid-generating enzyme agps in complex with inhibitor zinc69435460 (see paper)
27% identity, 52% coverage: 14:171/301 of query aligns to 127:290/560 of 5ae1A

query
sites
5ae1A

A

V

S

N

V

L

A

A

T

C

R

K

T

Y

P

N

L

L

C

C

L

I

R

I

-
x
P

-
x
I

G
|
G

S
x
T

K
x
S

D
x
V

F

S

F

Y

G
|
G

C
x
L

T

M

L

C

A

P

G

A

E

D

R

E

-

T

-

R

-

T

-

I

-

S

F

L

D

D

L

T

R

S

A

Q

N

M

R

N

G

R

V

I

V

L

A

W

Y

V

E

D

P

E

T

N

E

N

L

L

V

T

V

A

T

H

V

V

C

E

A

A

G

G

T

I

P

T

L

G

A

Q

E

E

L

L

E

E

A

R

V

Q

L

L

A

K

D

E

K

S

G

G

Q

Y

Y

C

L

T

A

G

F

H

E

E

P
|
P

P
x
D

H
x
S

F

L

G

-

P

E

G

F

A
x
S

T
|
T

V

V

G
|
G

C
x
W

V

I

T
x
S

A
x
T

G

R

L
x
A

S
|
S

G

G

P

M

R

K

A

N

S

I

A

Y

G

G

A

N

V

I

R

E

D

D

F

L

M

V

L

V

G

H

V

M

K

K

L

V

D

T

G

P

R

R

G

G

E

-

V

V

L

I

R

E

F

K

G

S

G

C

Q

Q

V

G

M

P

K

R

N

M

V

S

A

T

G

G

Y

P

D

D

V

I

S

H

R

H

L

F

V

I

A

M

G

G

S

S

L
x
E

G
|
G

T

T

L

L

G

G

I
x
V

L
x
I

L

T

E

E

V

A

S

T

L

I

K

K

V

I

L

R

P

P

R

T

P

P

binding flavin-adenine dinucleotide: P139 (vs. gap), I140 (vs. gap), G141 (= G26), G142 (= G27), G143 (= G28), T144 (≠ S29), S145 (≠ K30), V146 (≠ D31), G149 (= G34), L150 (≠ C35), P207 (= P86), D208 (≠ P87), S209 (≠ H88), S213 (≠ A93), T214 (= T94), G216 (= G96), G217 (= G97), W218 (≠ C98), S220 (≠ T100), T221 (≠ A101), A223 (≠ L103), S224 (= S104), E273 (≠ L154), G274 (= G155), V278 (≠ I159), I279 (≠ L160)

Sites not aligning to the query:

5adzA Ether lipid-generating enzyme agps in complex with inhibitor 1a (see paper)
27% identity, 52% coverage: 14:171/301 of query aligns to 130:293/566 of 5adzA

query
sites
5adzA

A

V

S

N

V

L

A

A

T

C

R

K

T

Y

P

N

L

L

C

C

L

I

R

I

-
x
P

-
x
I

G
|
G

S
x
T

K
x
S

D

V

F

S

F

Y

G
|
G

C

L

T

M

L

C

A

P

G

A

E

D

R

E

-

T

-

R

-

T

-

I

-

S

F

L

D

D

L

T

R

S

A

Q

N

M

R

N

G

R

V

I

V

L

A

W

Y

V

E

D

P

E

T

N

E

N

L

L

V

T

V

A

T

H

V

V

C

E

A

A

G

G

T

I

P

T

L

G

A

Q

E

E

L

L

E

E

A

R

V

Q

L

L

A

K

D

E

K

S

G

G

Q

Y

Y

C

L

T

A

G

F

H

E

E

P
|
P

P
x
D

H
x
S

F

L

G

-

P

E

G

F

A
x
S

T
|
T

V

V

G

G

G
|
G

C
x
W

V

I

T
x
S

A
x
T

G

R

L
x
A

S
|
S

G

G

P

M

R

K

A

N

S

I

A

Y

G

G

A

N

V

I

R

E

D

D

F

L

M

V

L

V

G

H

V

M

K

K

L

V

D

T

G

P

R

R

G

G

E

-

V

V

L

I

R

E

F

K

G

S

G

C

Q

Q

V

G

M

P

K

R

N

M

V

S

A

T

G

G

Y

P

D

D

V

I

S

H

R

H

L

F

V

I

A

M

G

G

S

S

L
x
E

G
|
G

T

T

L

L

G

G

I
x
V

L
x
I

L

T

E

E

V

A

S

T

L

I

K

K

V

I

L

R

P

P

R

T

P

P

binding flavin-adenine dinucleotide: P142 (vs. gap), I143 (vs. gap), G144 (= G26), G145 (= G27), G146 (= G28), T147 (≠ S29), S148 (≠ K30), G152 (= G34), P210 (= P86), D211 (≠ P87), S212 (≠ H88), S216 (≠ A93), T217 (= T94), G220 (= G97), W221 (≠ C98), S223 (≠ T100), T224 (≠ A101), A226 (≠ L103), S227 (= S104), E276 (≠ L154), G277 (= G155), V281 (≠ I159), I282 (≠ L160)
binding (3S)-3-(2-fluorophenyl)-N-((2-oxo-2,3-dihydro-1H-benzo[d]imidazol-5-yl)methyl)butanamide): D211 (≠ P87)

Sites not aligning to the query:

3pm9A Crystal structure of a putative dehydrogenase (rpa1076) from rhodopseudomonas palustris cga009 at 2.57 a resolution
35% identity, 28% coverage: 94:176/301 of query aligns to 140:222/465 of 3pm9A

query
sites
3pm9A

T
|
T

V

I

G
|
G

C

N

V

L

T
x
S

A
x
T

G

N

L
x
A

S
x
G

G

G

P

T

R

A

A

L

S

A

A

Y

G

G

A
x
L

V

A

R

R

D

D

F

M

M

A

L

L

G

G

V

V

K

E

L

V

D

L

G

A

R

D

G

G

E

R

V

V

L

M

R

N

F

L

G

L

G

S

Q

K

V

L

M

K

K

K

N

D

V

N

A

T

G

G

Y

Y

D

D

V

L

S

R

R

D

L

L

V

F

A

I

G

G

S

A

L
x
E

G
|
G

T

T

L

L

G

G

I
|
I

L
x
I

L

T

E

A

V

A

S

T

L

L

K

K

V

L

L

F

P

P

R

K

P

P

V

R

A

A

E

V

A

E

T

T

active site: A149 (≠ L103), L159 (≠ A113)
binding flavin-adenine dinucleotide: T140 (= T94), G142 (= G96), G143 (= G97), S146 (≠ T100), T147 (≠ A101), A149 (≠ L103), G150 (≠ S104), E200 (≠ L154), G201 (= G155), I205 (= I159), I206 (≠ L160)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 69, 70, 71, 72, 73, 74, 75, 76, 79, 80, 91, 133, 134, 135, 139, 423

8jdsA Crystal structure of mldhd in complex with pyruvate (see paper)
31% identity, 40% coverage: 57:177/301 of query aligns to 103:221/456 of 8jdsA

query
sites
8jdsA

E

D

L

F

V

S

V

V

T

V

V

V

C

E

A

P

G

G

T

V

P

T

L

R

A

K

E

A

L

L

E

N

A

T

V

H

L

L

A

R

D

D

K

S

G

G

Q

L

Y

W

L

F

A

P

F

V

E

D

P
|
P

P

-

H

-

F

-

G
|
G

P
x
A

G

D

A

A

T

S

V

L

G

C

G
|
G

C
x
M

V

A

T
x
A

A
x
T

G

G

L
x
A

S
|
S

G

G

P

T

R

N

R

A

A

V

S

R

A

Y

G

G

A

T

V

M

R

R

D

D

F

N

M

V

L

I

G

N

V

L

K

E

L

V

D

L

G

P

R

D

G

G

E

R

V

L

L

L

R

H

F

T

G

A

G

G

-

R

-

G

-

R

Q

H

V

Y

M

R

K

K

N

S

V

A

A

A

G

G

Y

Y

D

N

V

L

S

T

R

G

L

L

V

F

A

V

G

G

S

S

L
x
E

G
|
G

T

T

L

L

G

G

I

I

L
x
I

L

T

E

S

V

T

S

T

L

L

K

R

V

L

L

H

P

P

R

A

P

P

V

-

A

-

E

E

A

A

T

T

L

V

binding flavin-adenine dinucleotide: P132 (= P86), G133 (= G90), A134 (≠ P91), G140 (= G97), M141 (≠ C98), A143 (≠ T100), T144 (≠ A101), A146 (≠ L103), S147 (= S104), E200 (≠ L154), G201 (= G155), I206 (≠ L160)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 32, 68, 70, 71, 72, 73, 74, 78, 79, 90, 323, 414, 415, 451
binding manganese (ii) ion: 370, 377, 414
binding pyruvic acid: 319, 370, 377, 415

8jdeA Crystal structure of mldhd in complex with d-lactate (see paper)
31% identity, 40% coverage: 57:177/301 of query aligns to 103:221/455 of 8jdeA

query
sites
8jdeA

E

D

L

F

V

S

V

V

T

V

V

V

C

E

A

P

G

G

T

V

P

T

L

R

A

K

E

A

L

L

E

N

A

T

V

H

L

L

A

R

D

D

K

S

G

G

Q

L

Y

W

L

F

A

P

F

V

E

D

P
|
P

P

-

H

-

F

-

G
|
G

P
x
A

G

D

A

A

T

S

V

L

G

C

G
|
G

C
x
M

V

A

T
x
A

A
x
T

G

G

L
x
A

S
|
S

G

G

P

T

R

N

R

A

A

V

S

R

A

Y

G

G

A

T

V

M

R

R

D

D

F

N

M

V

L

I

G

N

V

L

K

E

L

V

D

L

G

P

R

D

G

G

E

R

V

L

L

L

R

H

F

T

G

A

G

G

-

R

-

G

-

R

Q

H

V

Y

M

R

K

K

N

S

V

A

A

A

G

G

Y

Y

D

N

V

L

S

T

R

G

L

L

V

F

A

V

G

G

S

S

L
x
E

G
|
G

T

T

L

L

G

G

I

I

L
x
I

L

T

E

S

V

T

S

T

L

L

K

R

V

L

L

H

P

P

R

A

P

P

V

-

A

-

E

E

A

A

T

T

L

V

binding flavin-adenine dinucleotide: P132 (= P86), G133 (= G90), A134 (≠ P91), G140 (= G97), M141 (≠ C98), A143 (≠ T100), T144 (≠ A101), A146 (≠ L103), S147 (= S104), E200 (≠ L154), G201 (= G155), I206 (≠ L160)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 68, 70, 71, 72, 73, 74, 78, 79, 90, 322, 413, 414, 450
binding lactic acid: 318, 369, 376, 414
binding manganese (ii) ion: 369, 376, 413

8jdpA Crystal structure of h405a mldhd in complex with d-2-hydroxyisovaleric acid (see paper)
31% identity, 40% coverage: 57:177/301 of query aligns to 103:221/455 of 8jdpA

query
sites
8jdpA

E

D

L

F

V

S

V

V

T

V

V

V

C

E

A

P

G

G

T

V

P

T

L

R

A

K

E

A

L

L

E

N

A

T

V

H

L

L

A

R

D

D

K

S

G

G

Q

L

Y

W

L

F

A

P

F

V

E

D

P
|
P

P

-

H

-

F

-

G
|
G

P
x
A

G

D

A

A

T

S

V

L

G

C

G
|
G

C
x
M

V

A

T
x
A

A
x
T

G

G

L
x
A

S
|
S

G

G

P

T

R

N

R

A

A

V

S

R

A

Y

G

G

A

T

V

M

R

R

D

D

F

N

M

V

L

I

G

N

V

L

K

E

L

V

D

L

G

P

R

D

G

G

E

R

V

L

L

L

R

H

F

T

G

A

G

G

-

R

-

G

-

R

Q

H

V

Y

M

R

K

K

N

S

V

A

A

A

G

G

Y

Y

D

N

V

L

S

T

R

G

L

L

V

F

A

V

G

G

S

S

L
x
E

G
|
G

T

T

L

L

G

G

I

I

L
x
I

L

T

E

S

V

T

S

T

L

L

K

R

V

L

L

H

P

P

R

A

P

P

V

-

A

-

E

E

A

A

T

T

L

V

binding flavin-adenine dinucleotide: P132 (= P86), G133 (= G90), A134 (≠ P91), G140 (= G97), M141 (≠ C98), A143 (≠ T100), T144 (≠ A101), A146 (≠ L103), S147 (= S104), E200 (≠ L154), G201 (= G155), I206 (≠ L160)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 68, 70, 71, 72, 73, 74, 78, 79, 90, 369, 413, 450
binding deaminohydroxyvaline: 319, 414

8jduA Crystal structure of mldhd in complex with 2-ketovaleric acid (see paper)
31% identity, 40% coverage: 57:177/301 of query aligns to 103:221/455 of 8jduA

query
sites
8jduA

E

D

L

F

V

S

V

V

T

V

V

V

C

E

A

P

G

G

T

V

P

T

L

R

A

K

E

A

L

L

E

N

A

T

V

H

L

L

A

R

D

D

K

S

G

G

Q

L

Y

W

L

F

A

P

F

V

E

D

P
|
P

P

-

H

-

F

-

G
|
G

P
x
A

G

D

A

A

T

S

V

L

G

C

G
|
G

C
x
M

V

A

T
x
A

A
x
T

G

G

L
x
A

S
|
S

G

G

P

T

R

N

R

A

A

V

S

R

A

Y

G

G

A

T

V

M

R

R

D

D

F

N

M

V

L

I

G

N

V

L

K

E

L

V

D

L

G

P

R

D

G

G

E

R

V

L

L

L

R

H

F

T

G

A

G

G

-

R

-

G

-

R

Q

H

V

Y

M

R

K

K

N

S

V

A

A

A

G

G

Y

Y

D

N

V

L

S

T

R

G

L

L

V

F

A

V

G

G

S

S

L
x
E

G
|
G

T

T

L

L

G

G

I

I

L
x
I

L

T

E

S

V

T

S

T

L

L

K

R

V

L

L

H

P

P

R

A

P

P

V

-

A

-

E

E

A

A

T

T

L

V

binding flavin-adenine dinucleotide: P132 (= P86), G133 (= G90), A134 (≠ P91), G140 (= G97), M141 (≠ C98), A143 (≠ T100), T144 (≠ A101), A146 (≠ L103), S147 (= S104), E200 (≠ L154), G201 (= G155), I206 (≠ L160)

Sites not aligning to the query:

binding 2-oxopentanoic acid: 318, 322, 369, 376, 414
binding flavin-adenine dinucleotide: 68, 70, 71, 72, 73, 74, 78, 79, 90, 322, 413, 450
binding manganese (ii) ion: 369, 376, 413

Query Sequence

>WP_004322877.1 NCBI__GCF_000310185.1:WP_004322877.1
MTMIEKEWAERVRASVATRTPLCLRGGGSKDFFGCTLAGERFDLRANRGVVAYEPTELVV
TVCAGTPLAELEAVLADKGQYLAFEPPHFGPGATVGGCVTAGLSGPRRASAGAVRDFMLG
VKLLDGRGEVLRFGGQVMKNVAGYDVSRLVAGSLGTLGILLEVSLKVLPRPVAEATLRFE
LDEASALRRVNEWGGQPLPVSATLWQHGVLHLRLSGAEAAVQAACARLGGEALDDATAQA
LWTAAREQTLPGFALEEGEVLWRLSLPSTAPALGLPGRQLVEWGGAQRWLAGAIDAQAVC
A

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory