SitesBLAST – Find functional sites

 

SitesBLAST

Comparing WP_004385842.1 NCBI__GCF_000319285.1:WP_004385842.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 4 hits to proteins with known functional sites (download)

P69783 PTS system glucose-specific EIIA component; EIIA-Glc; EIII-Glc; Glucose-specific phosphotransferase enzyme IIA component from Escherichia coli (strain K12) (see 6 papers)
91% identity, 100% coverage: 1:167/167 of query aligns to 1:169/169 of P69783

query
sites
P69783
M
|
M
G
 
G
L
 
L
F
|
F
S
 
D
K
 
K
L
 
L
-
 
K
-
 
S
F
 
L
G
 
V
D
 
S
K
 
D
G
 
D
N
 
K
S
 
K
D
 
D
T
 
T
G
 
G
T
 
T
I
 
I
E
 
E
I
 
I
V
 
I
A
 
A
P
 
P
L
 
L
S
 
S
G
 
G
E
 
E
I
 
I
V
 
V
N
 
N
I
 
I
E
 
E
D
 
D
V
 
V
P
 
P
D
 
D
V
 
V
V
 
V
F
 
F
A
 
A
E
 
E
K
 
K
I
 
I
V
 
V
G
 
G
D
 
D
G
 
G
I
 
I
A
 
A
I
 
I
K
 
K
P
 
P
T
 
T
G
 
G
N
 
N
K
 
K
M
 
M
V
 
V
A
 
A
P
 
P
V
 
V
D
 
D
G
 
G
T
 
T
I
 
I
G
 
G
K
 
K
I
 
I
F
 
F
E
 
E
T
 
T
N
 
N
H
|
H
A
 
A
F
 
F
S
 
S
I
 
I
E
 
E
S
 
S
D
 
D
S
 
S
G
 
G
I
 
V
E
 
E
L
 
L
F
 
F
V
 
V
H
|
H
F
 
F
G
 
G
I
 
I
D
 
D
T
 
T
V
 
V
E
 
E
L
 
L
K
 
K
G
 
G
E
 
E
G
 
G
F
 
F
K
 
K
R
 
R
I
 
I
A
 
A
E
 
E
E
 
E
G
 
G
Q
 
Q
R
 
R
V
 
V
K
 
K
K
 
V
G
 
G
D
 
D
P
 
T
V
 
V
I
 
I
E
 
E
F
 
F
D
 
D
L
 
L
P
 
P
L
 
L
L
 
L
E
 
E
E
 
E
K
 
K
A
 
A
K
 
K
S
 
S
T
 
T
L
 
L
T
 
T
P
 
P
V
 
V
V
 
V
I
 
I
S
 
S
N
 
N
M
 
M
D
 
D
E
 
E
I
 
I
K
 
K
E
 
E
L
 
L
T
 
I
K
 
K
L
 
L
S
 
S
G
 
G
S
 
S
V
 
V
T
 
T
V
 
V
G
 
G
E
 
E
T
 
T
P
 
P
V
 
V
I
 
I
R
 
R
I
 
I
K
 
K
K
 
K

1glcF Cation promoted association (cpa) of a regulatory and target protein is controlled by phosphorylation (see paper)
91% identity, 99% coverage: 2:167/167 of query aligns to 1:161/161 of 1glcF

query
sites
1glcF
G
 
G
L
 
L
F
 
F
S
 
D
K
 
K
L
 
L
F
 
-
G
 
-
D
 
-
K
 
-
G
 
-
N
 
K
S
 
S
D
 
L
T
 
V
G
 
S
T
 
T
I
 
I
E
 
E
I
 
I
V
 
I
A
 
A
P
 
P
L
 
L
S
 
S
G
 
G
E
 
E
I
 
I
V
 
V
N
 
N
I
 
I
E
 
E
D
 
D
V
 
V
P
 
P
D
 
D
V
 
V
V
 
V
F
 
F
A
 
A
E
 
E
K
 
K
I
 
I
V
 
V
G
 
G
D
 
D
G
 
G
I
 
I
A
 
A
I
 
I
K
 
K
P
 
P
T
 
T
G
 
G
N
 
N
K
 
K
M
 
M
V
 
V
A
 
A
P
 
P
V
 
V
D
 
D
G
 
G
T
 
T
I
 
I
G
 
G
K
 
K
I
 
I
F
 
F
E
 
E
T
 
T
N
 
N
H
|
H
A
 
A
F
 
F
S
 
S
I
 
I
E
 
E
S
 
S
D
 
D
S
 
S
G
 
G
I
 
V
E
 
E
L
 
L
F
 
F
V
 
V
H
|
H
F
 
F
G
 
G
I
 
I
D
 
D
T
 
T
V
 
V
E
 
E
L
 
L
K
 
K
G
 
G
E
 
E
G
 
G
F
 
F
K
 
K
R
 
R
I
 
I
A
 
A
E
 
E
E
 
E
G
 
G
Q
 
Q
R
 
R
V
 
V
K
 
K
K
 
V
G
 
G
D
 
D
P
 
T
V
 
V
I
 
I
E
 
E
F
 
F
D
 
D
L
 
L
P
 
P
L
 
L
L
 
L
E
 
E
E
 
E
K
 
K
A
 
A
K
 
K
S
 
S
T
 
T
L
 
L
T
 
T
P
 
P
V
 
V
V
 
V
I
 
I
S
 
S
N
 
N
M
 
M
D
 
D
E
 
E
I
 
I
K
 
K
E
 
E
L
 
L
T
 
I
K
 
K
L
 
L
S
 
S
G
 
G
S
 
S
V
 
V
T
 
T
V
 
V
G
 
G
E
 
E
T
 
T
P
 
P
V
 
V
I
 
I
R
 
R
I
 
I
K
 
K
K
 
K

1o2fA Complex of enzyme iiaglc and iibglc phosphocarrier protein hpr from escherichia coli nmr, restrained regularized mean structure (see paper)
97% identity, 90% coverage: 18:167/167 of query aligns to 1:150/150 of 1o2fA

query
sites
1o2fA
T
 
T
I
 
I
E
 
E
I
 
I
V
 
I
A
 
A
P
 
P
L
 
L
S
 
S
G
 
G
E
 
E
I
 
I
V
 
V
N
 
N
I
 
I
E
 
E
D
 
D
V
 
V
P
 
P
D
 
D
V
 
V
V
 
V
F
 
F
A
 
A
E
 
E
K
 
K
I
 
I
V
 
V
G
 
G
D
 
D
G
 
G
I
 
I
A
 
A
I
 
I
K
 
K
P
 
P
T
 
T
G
 
G
N
 
N
K
 
K
M
 
M
V
 
V
A
 
A
P
 
P
V
 
V
D
 
D
G
 
G
T
 
T
I
 
I
G
 
G
K
 
K
I
 
I
F
 
F
E
 
E
T
|
T
N
 
N
H
|
H
A
 
A
F
 
F
S
 
S
I
 
I
E
 
E
S
 
S
D
 
D
S
 
S
G
 
G
I
 
V
E
 
E
L
 
L
F
 
F
V
 
V
H
|
H
F
 
F
G
 
G
I
 
I
D
|
D
T
 
T
V
 
V
E
 
E
L
 
L
K
 
K
G
 
G
E
 
E
G
 
G
F
 
F
K
 
K
R
 
R
I
 
I
A
 
A
E
 
E
E
 
E
G
 
G
Q
 
Q
R
 
R
V
 
V
K
 
K
K
 
V
G
 
G
D
 
D
P
 
T
V
 
V
I
 
I
E
 
E
F
 
F
D
 
D
L
 
L
P
 
P
L
 
L
L
 
L
E
 
E
E
 
E
K
 
K
A
 
A
K
 
K
S
 
S
T
 
T
L
 
L
T
 
T
P
 
P
V
 
V
V
 
V
I
 
I
S
 
S
N
 
N
M
 
M
D
 
D
E
 
E
I
 
I
K
 
K
E
 
E
L
 
L
T
 
I
K
 
K
L
 
L
S
 
S
G
 
G
S
 
S
V
 
V
T
 
T
V
 
V
G
 
G
E
 
E
T
 
T
P
 
P
V
 
V
I
 
I
R
 
R
I
 
I
K
 
K
K
 
K

P09323 PTS system N-acetylglucosamine-specific EIICBA component; EIICBA-Nag; EII-Nag; EC 2.7.1.193 from Escherichia coli (strain K12) (see paper)
50% identity, 89% coverage: 20:167/167 of query aligns to 500:648/648 of P09323

query
sites
P09323
E
 
E
I
 
L
V
 
V
A
 
S
P
 
P
L
 
I
S
 
T
G
 
G
E
 
D
I
 
V
V
 
V
N
 
A
I
 
L
E
 
D
D
 
Q
V
 
V
P
 
P
D
 
D
V
 
E
V
 
A
F
 
F
A
 
A
E
 
S
K
 
K
I
 
A
V
 
V
G
 
G
D
 
D
G
 
G
I
 
V
A
 
A
I
 
V
K
 
K
P
 
P
T
 
T
G
 
D
N
 
K
K
 
I
M
 
V
V
 
V
A
 
S
P
 
P
V
 
A
D
 
A
G
 
G
T
 
T
I
 
I
G
 
V
K
 
K
I
 
I
F
 
F
E
 
N
T
 
T
N
 
N
H
 
H
A
 
A
F
 
F
S
 
C
I
 
L
E
 
E
S
 
T
D
 
E
S
 
K
G
 
G
I
 
A
E
 
E
L
 
I
F
 
V
V
 
V
H
 
H
F
 
M
G
 
G
I
 
I
D
 
D
T
 
T
V
 
V
E
 
A
L
 
L
K
 
E
G
 
G
E
 
K
G
 
G
F
 
F
K
 
K
R
 
R
I
 
L
A
 
V
E
 
E
E
 
E
G
 
G
Q
 
A
R
 
Q
V
 
V
K
 
S
K
 
A
G
 
G
D
 
Q
P
 
P
V
 
I
I
 
L
E
 
E
F
 
M
D
 
D
L
 
L
P
 
D
L
 
Y
L
 
L
E
 
N
E
 
A
K
 
N
A
 
A
K
 
R
S
 
S
T
 
M
L
 
I
T
 
S
P
 
P
V
 
V
V
 
V
I
 
C
S
 
S
N
 
N
M
 
I
D
 
D
E
 
D
I
 
F
K
 
S
E
 
G
L
 
L
-
 
I
T
 
I
K
 
K
L
 
A
S
 
Q
G
 
G
S
 
H
V
 
I
T
 
V
V
 
A
G
 
G
E
 
Q
T
 
T
P
 
P
V
 
L
I
 
Y
R
 
E
I
 
I
K
 
K
K
 
K

Sites not aligning to the query:

Query Sequence

>WP_004385842.1 NCBI__GCF_000319285.1:WP_004385842.1
MGLFSKLFGDKGNSDTGTIEIVAPLSGEIVNIEDVPDVVFAEKIVGDGIAIKPTGNKMVA
PVDGTIGKIFETNHAFSIESDSGIELFVHFGIDTVELKGEGFKRIAEEGQRVKKGDPVIE
FDLPLLEEKAKSTLTPVVISNMDEIKELTKLSGSVTVGETPVIRIKK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory