SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_004511507.1 NCBI__GCF_000012925.1:WP_004511507.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P9WP55 O-acetylserine sulfhydrylase; OAS sulfhydrylase; OASS; Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine-specific cysteine synthase; Sulfide-dependent cysteine synthase; EC 2.5.1.47 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
72% identity, 99% coverage: 4:308/309 of query aligns to 3:307/310 of P9WP55

query
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P9WP55

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K44 (= K45) modified: N6-(pyridoxal phosphate)lysine
N74 (= N75) binding pyridoxal 5'-phosphate
GTGGT 178:182 (= GTGGT 179:183) binding pyridoxal 5'-phosphate
S266 (= S267) binding pyridoxal 5'-phosphate

2q3dA 2.2 a resolution crystal structure of o-acetylserine sulfhydrylase (oass) from mycobacterium tuberculosis in complex with the reaction intermediate alpha-aminoacrylate (see paper)
72% identity, 98% coverage: 4:306/309 of query aligns to 3:305/306 of 2q3dA

query
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2q3dA

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active site: K44 (= K45), S266 (= S267), P293 (= P294)
binding 2-[(3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl)-amino]-propionic acid: K44 (= K45), T71 (= T72), S72 (= S73), N74 (= N75), T75 (= T76), Q144 (= Q145), V177 (= V178), G178 (= G179), T179 (= T180), G180 (= G181), T182 (= T183), G222 (= G223), I223 (= I224), S266 (= S267), P293 (= P294), D294 (= D295)

3zeiA Structure of the mycobacterium tuberculosis o-acetylserine sulfhydrylase (oass) cysk1 in complex with a small molecule inhibitor (see paper)
72% identity, 96% coverage: 4:301/309 of query aligns to 3:300/300 of 3zeiA

query
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active site: K44 (= K45), S266 (= S267), P293 (= P294)
binding 3-[(Z)-[(5Z)-5-[[2-(2-hydroxy-2-oxoethyloxy)phenyl]methylidene]-3-methyl-4-oxidanylidene-1,3-thiazolidin-2-ylidene]amino]benzoic acid: T71 (= T72), S72 (= S73), I126 (= I127), Q144 (= Q145), F145 (= F146), K215 (≠ P216), G222 (= G223), A225 (= A226), F227 (= F228)
binding pyridoxal-5'-phosphate: K44 (= K45), N74 (= N75), V177 (= V178), G178 (= G179), T179 (= T180), G180 (= G181), T182 (= T183), G222 (= G223), S266 (= S267), P293 (= P294), D294 (= D295)

2q3cA 2.1 a resolution crystal structure of o-acetylserine sulfhydrylase (oass) holoenzyme from mycobacterium tuberculosis in complex with the inhibitory peptide dfsi (see paper)
72% identity, 96% coverage: 4:301/309 of query aligns to 3:300/300 of 2q3cA

query
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2q3cA

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active site: K44 (= K45), S266 (= S267), P293 (= P294)
binding : T71 (= T72), S72 (= S73), G73 (= G74), T75 (= T76), M122 (= M123), Q144 (= Q145), K215 (≠ P216), G222 (= G223), A225 (= A226)

4lmaA Crystal structure analysis of o-acetylserine sulfhydrylase cysk1 from microcystis aeruginosa 7806 (see paper)
62% identity, 99% coverage: 3:308/309 of query aligns to 2:310/318 of 4lmaA

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4lmaA

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G

G

K

R

T

T

P

P

L

L

L

V

R

Q

L

L

R

N

K

R

V

I

-

P

-

Q

A

A

A

E

G

G

I

C

E

L

A

G

D

R

I

I

V

V

A

M

K

K

L

L

E

E

S

G

F

M

N

N

P

P

G

A

S

S

V

V

K
|
K

D

D

R

R

I

I

G

G

V

T

S

H

M

M

I

I

R

N

D

S

A

A

E

E

G

K

R

A

G

G

L

L

I

I

N

N

K

P

D

E

T

T

-

T

V

V

I

L

I

V

E

E

P

P

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

A

A

L

L

A

A

F

M

V

T

C

A

A

A

R

K

G

G

Y

Y

R

R

L

L

V

I

L

L

T

T

M

M

P

P

D

E

T

T

M

M

S

S

V

L

E

E

R

R

N

A

L

M

L

L

K

K

A

A

Y

Y

G

G

A

A

E

T

L

L

V

E

L

L

T

T

R

P

G

G

A

S

E

Q

G

G

M

M

K

K

G

G

A

A

I

I

N

L

K

R

A

A

E

Q

L

I

A

V

A

D

D

S

I

I

P

P

N

G

S

A

F

Y

I

M

P

L

Q

Q

F

F

R

R

N

N

P

P

A

S

N

N

P

P

H

E

I

I

H

H

R

R

F

L

T

T

A

A

E

E

I

I

W

W

T

Q

D

D

T

T

D

E

G

G

K

Q

V

V

D

D

V

F

F

I

V

V

A

A

G

G

V

V

G
|
G

T
|
T

G
|
G

G

G

T
|
T

V

I

T

T

G

G

V

V

G

A

E

E

V

V

L

I

K

K

K

S

R

R

N

K

P

P

G

S

V

F

K

Q

I

V

A

A

V

V

E

E

P

P

F

F

D

N

S

S

P

P

V

V

L

I

S

S

G

G

H

N

P

P

A

G

P

P

H

H

K

K

I

I

Q

Q

G
|
G

I

I

G

G

A

A

G

G

F

F

V

I

P

P

E

E

V

V

L

L

N

R

R

T

E

D

A

L

I

I

D

D

E

E

I

V

F

I

T

T

V

V

R

S

N

D

E

E

A

A

L

F

E

Q

T

F

A

G

R

R

L

L

A

A

R

K

E

E

G

G

L

L

V

S

G

G

I

I

S
|
S

A

S

G

G

A

A

A

N

A

L

F

C

A

A

L

I

E

Q

V

L

A

A

R

Q

R

R

P

P

E

E

N

N

K

E

G

G

K

K

R

L

I

I

V

V

V

I

L

Q

P
|
P

D

S

T

F

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

V

L

L

M

F

F

Q

Q

D

N

active site: K46 (= K45), S269 (= S267)
binding pyridoxal-5'-phosphate: K46 (= K45), N77 (= N75), G181 (= G179), T182 (= T180), G183 (= G181), T185 (= T183), G225 (= G223), S269 (= S267), P296 (= P294)

4lmbA Crystal structure analysis of o-acetylserine sulfhydrylase cysk2 complexed with cystine from microcystis aeruginosa 7806 (see paper)
64% identity, 99% coverage: 4:308/309 of query aligns to 3:310/310 of 4lmbA

query
sites
4lmbA

I

I

Y

A

E

R

N

D

L

I

T

T

D

Q

L

L

I

V

G

G

K

R

T

T

P

P

L

L

L

V

R

Q

L

L

R

N

K

R

-

I

-

P

V

V

A

A

A

E

G

G

I

V

E

K

A

A

D

R

I

I

V

V

A

V

K

K

L

L

E

E

S

S

F

M

N

N

P

P

G

A

S

S

V

V

K
|
K

D

D

R

R

I

I

G

G

V

V

S

S

M

M

I

V

R

E

D

D

A

A

E

E

G

A

R

A

G

G

L

L

I

I

N

H

K

P

D

D

-

K

T

T

V

I

I

L

I

V

E

E

P

P

T
|
T

S
|
S

G

G

N
|
N

T
|
T

G

G

I

I

A

A

L

L

A

A

F

M

V

V

C

A

A

A

R

K

G

G

Y

Y

R

R

L

L

V

V

L

L

T

T

M

M

P

P

D

E

T

T

M
|
M

S

S

V

L

E

E

R

R

N

A

L

M

L

L

K

K

A

A

Y

Y

G

G

A

A

E

Q

L

L

V

E

L

L

T

T

R

P

G

G

A

S

E

Q

G

G

M
|
M

K

E

G

G

A

A

I

I

N

T

K

R

A

A

E

E

L

I

A

V

A

E

D

N

I

T

P

P

N

N

S

A

F

Y

I

S

P

L

Q

Q

Q
|
Q

F
|
F

R

R

N

N

P

P

A

A

N

N

P

P

H

K

I

I

H

H

R

R

F

E

T

T

A

A

E

E

I

I

W

W

T

A

D

D

T

T

D

D

G

G

K

L

V

V

D

D

V

I

F

V

V

I

A

G

G

G

V
|
V

G
|
G

T
|
T

G
|
G

G

G

T
|
T

V

I

T

T

G

G

V

I

G

A

E

E

V

T

L

I

K

K

K

P

R

R

N

R

P

P

G

Q

V

F

K

Q

I

A

I

I

A

A

V

V

E

E

P

P

F

S

D

N

S

S

P

P

V

V

L

L

S

S

G

G

H

Q

P

P

A

G

P

P

H

H

K

K

I

I

Q
|
Q

G
|
G

I
|
I

G

G

A
|
A

G

G

F

F

V

I

P

P

E

A

V

I

L

F

N

R

R

P

E

E

A

L

I

I

D

D

E

E

I

V

F

I

T

I

V

V

R

D

N

D

E

T

E

E

A

A

L

F

E

A

T

Y

A

A

R

R

L

L

A

A

R

R

E

Q

E

E

G

G

L

L

V

S

G

G

I

I

S
|
S

A

A

G

G

A

A

A

L

F

W

A

A

L

I

E

Q

V

V

A

G

R

K

R

R

P

P

E

E

N

N

K

E

G

D

K

K

R

L

I

I

V

V

V

M

V

I

L

Q

P
|
P

D

S

T

F

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

V

A

L

L

F

F

Q

K

D

D

active site: K46 (= K45), S269 (= S267)
binding cysteine: K46 (= K45), T74 (= T72), S75 (= S73), N77 (= N75), T78 (= T76), M101 (= M99), M125 (= M123), M125 (= M123), Q147 (= Q145), F148 (= F146), Q224 (= Q222), G225 (= G223), G225 (= G223), I226 (= I224), A228 (= A226)
binding pyridoxal-5'-phosphate: K46 (= K45), N77 (= N75), V180 (= V178), G181 (= G179), T182 (= T180), G183 (= G181), T185 (= T183), G225 (= G223), S269 (= S267), P296 (= P294)

5xoqA Crystal structure of o-acetylserine sulfhydrylase with bound transcription factor peptide inhibitor from planctomyces limnophilus
62% identity, 99% coverage: 4:308/309 of query aligns to 4:308/310 of 5xoqA

query
sites
5xoqA

I

I

Y

F

E

K

N

D

L

N

T

S

D

E

L

S

I

I

G

G

K

R

T

T

P

P

L

L

L

V

R

Q

L

I

R

N

K

R

V

L

A

T

A

A

G

G

I

L

E

S

A

S

D

R

I

V

V

L

A

A

K

K

L

I

E

E

S

G

F

R

N

N

P

P

G

A

G

Y

S

S

V

V

K

K

D

C

R

R

I

I

G

G

V

A

S

A

M

M

I

I

R

W

D

D

A

A

E

E

G

Q

R

S

G

G

L

K

I

L

N

K

K

P

D

G

T

M

V

H

I

V

E

E

P

P

T
|
T

S
|
S

G
|
G

N

N

T
|
T

G

G

I

I

A

A

L

L

A

A

F

F

V

V

C

C

A

A

R

R

G

G

Y

Y

R

K

L

L

V

T

L

L

T

T

M

M

P

P

D

E

T

T

M

M

S

S

V

I

E

E

R

R

N

M

L

M

L

L

K

K

A

S

Y

F

G

G

A

A

E

D

L

L

V

V

L

L

T

T

R

P

G

G

A

A

E

D

G

G

M
|
M

K

K

G

G

A

A

I

I

N

S

K

K

A

A

E

E

L

L

A

A

D

Q

I

-

P

P

N

G

S

W

F

F

I

I

P

P

Q

Q

Q
|
Q

F

F

R

K

N

N

P

P

A

A

N

N

P

P

H

A

I

I

H

H

R

V

F

K

T

T

A

G

E

P

E

E

I

I

W

W

T

N

D

D

T

T

D

E

G

G

K

Q

V

V

D

D

V

V

F

F

V

V

A

A

G

G

V

V

G

G

T

T

G

G

T

T

V

I

T

T

G

G

V

V

G

A

E

R

V

F

L

L

K

K

-

H

-

E

K

K

R

K

N

H

P

P

G

-

V

V

K

H

I

V

A

A

V

V

E

E

P

P

F

A

D

A

S

S

P

P

V

V

L

L

S

A

G

G

H

P

P

A

A

G

P
x
R

H
|
H

K

K

I

I

Q
|
Q

G
|
G

I

I

G

G

A
|
A

G

G

F

F

V

V

P

P

E

D

V

T

L

F

N

D

R

R

E

S

A

V

I

V

D

D

E

E

I

I

F

L

T

S

V

V

R

T

N

D

E

D

E

E

A

A

L

I

E

E

T

T

A

A

R

R

R

K

L

L

A

A

R

M

E

E

G

G

L

I

L

S

V

C

G

G

I

I

S

S

A

C

G

G

A

A

A

M

F

A

A

G

A

A

L

L

E

K

V

V

A

A

R

A

R

R

P

P

E

E

N

F

K

A

G

G

K

K

R

T

I

I

V

V

V

T

V

V

L

L

P

P

D

D

T

A

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

V

A

L

L

F

F

Q

E

D

N

binding : T72 (= T72), S73 (= S73), G74 (= G74), T76 (= T76), M123 (= M123), Q144 (= Q145), R218 (≠ P218), H219 (= H219), Q222 (= Q222), G223 (= G223), A226 (= A226)

4aecA Crystal structure of the arabidopsis thaliana o-acetyl-serine-(thiol)- lyasE C (see paper)
61% identity, 98% coverage: 4:307/309 of query aligns to 13:317/323 of 4aecA

query
sites
4aecA

I

I

Y

A

E

D

N

N

L

V

T

S

D

Q

L

L

I

I

G

G

K

K

T

T

P

P

L

M

L

V

R

Y

L

L

R

N

K

S

V

I

A

A

A

K

G

G

I

C

E

V

A

A

D

N

I

I

V

A

A

A

K

K

L

L

E

E

S

I

F

M

N

E

P

P

G

C

S

S

V

V

K
|
K

D

D

R

R

I

I

G

G

V

Y

S

S

M

M

I

V

R

T

D

D

A

A

E

E

G

Q

R

K

G

G

L

F

I

I

N

S

K

P

-

G

D

K

T

S

V

V

I

L

I

V

E

E

P

P

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

A

G

L

L

A

A

F

F

V

I

C

A

A

A

A

S

R

R

G

G

Y

Y

R

R

L

L

V

I

L

L

T

T

M

M

P

P

D

A

T

S

M

M

S

S

V

M

E

E

R

R

N

V

L

L

K

K

A

A

Y

F

G

G

A

A

E

E

L

L

V

V

L

L

T

T

R

D

G

P

A

A

E

K

G

G

M

M

K

T

G

G

A

A

I

V

N

Q

K

K

A

A

E

E

L

I

A

L

A

K

D

N

I

T

P

P

N

D

S

A

F

Y

I

M

P

L

Q

Q

F

F

R

D

N

N

P

P

A

A

N

N

P

P

H

K

I

I

H

H

R

Y

F

E

T

T

A

G

E

P

E

E

I

I

W

W

T

D

D

D

T

T

D

K

G

G

K

K

V

V

D

D

V

I

F

F

V

V

A

A

G

G

V
x
I

G
|
G

T
|
T

G
|
G

T
|
T

V

I

T

T

G

G

V

V

G

G

E

R

V

F

L

I

K

K

K

E

R

K

N

N

P

P

G

K

V

T

K

Q

I

V

I

I

A

G

V

V

E

E

P

P

F

T

D

E

S

S

P

D

V

I

L

L

S

S

G

G

H

K

P

P

A

G

P

P

H

H

K

K

I

I

Q

Q

G
|
G

I

I

G

G

A

A

G

G

F

F

V

I

P

P

E

K

V

N

L

L

N

D

R

Q

E

K

A

I

I

M

D

D

E

E

I

V

F

I

T

A

V

I

R

S

N

S

E

E

A

A

L

I

E

E

T

T

A

A

R

K

R

Q

L

L

A

A

R

L

E

K

E

E

G

G

L

L

L

M

V

V

G

G

I

I

S
|
S

A

S

G

G

A

A

F

A

A

A

L

I

E

K

V

V

A

A

R

K

R

R

P

P

E

E

N

N

K

A

G

G

K

K

R

L

I

I

V

A

V

V

L

F

P
|
P

D

S

T

F

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

V

P

L

L

F

F

Q

Q

active site: K54 (= K45), S277 (= S267)
binding pyridoxal-5'-phosphate: K54 (= K45), N85 (= N75), I188 (≠ V178), G189 (= G179), T190 (= T180), G191 (= G181), G192 (= G182), T193 (= T183), G233 (= G223), S277 (= S267), P304 (= P294)

P47999 Cysteine synthase, chloroplastic/chromoplastic; At.OAS.7-4; Beta-substituted Ala synthase 2;1; ARAth-Bsas2;1; CSase B; AtCS-B; CS-B; O-acetylserine (thiol)-lyase; O-acetylserine sulfhydrylase; OAS-TL B; cpACS1; EC 2.5.1.47 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
61% identity, 99% coverage: 4:308/309 of query aligns to 75:380/392 of P47999

query
sites
P47999

I

I

Y

A

E

D

N

N

L

A

T

A

D

Q

L

L

I

I

G

G

K

K

T

T

P

P

L

M

L

V

R

Y

L

L

R

N

K

N

V

V

A

V

A

K

G

G

I

C

E

V

A

A

D

S

I

V

V

A

A

A

K

K

L

L

E

E

S

I

F

M

N

E

P

P

G

C

S

S

V

V

K

K

D

D

R

R

I

I

G

G

V

Y

S

S

M

M

I

I

R

T

D

D

A

A

E

E

G

E

R

K

G

G

L

L

I

I

N

T

K

P

-

G

D

K

T

S

V

V

I

L

I

V

E

E

P

S

T

T

S

S

G

G

N

N

T

T

G

G

I

I

A

G

L

L

A

A

F

F

V

I

C

A

A

A

A

S

R

K

G

G

Y

Y

R

K

L

L

V

I

L

L

T

T

M

M

P

P

D

A

T

S

M

M

S

S

V

L

E

E

R

R

N

V

L

L

K

R

A

A

Y

F

G

G

A

A

E

E

L

L

V

V

L

L

T

T

R

E

G

P

A

A

E

K

G

G

M

M

K

T

G

G

A

A

I

I

N

Q

K

K

A

A

E

E

L

I

A

L

A

K

D

K

I

T

P

P

N

N

S

S

F

Y

I

M

P

L

Q

Q

F

F

R

D

N

N

P

P

A

A

N

N

P

P

H

K

I

I

H

H

R

Y

F

E

T

T

A

G

E

P

E

E

I

I

W

W

T

E

D

D

T

T

D

R

G

G

K

K

V

I

D

D

V

I

F

L

V

V

A

A

G

G

V

I

G

G

T

T

G

G

T

T

V

I

T

T

G

G

V

V

G

G

E

R

V

F

L

I

K

K

K

E

R

R

N

K

P

P

G

E

V

L

K

K

I

V

I

I

A

G

V

V

E

E

P

P

F

T

D

E

S

S

P

A

V

I

L

L

S

S

G

G

H

K

P

P

A

G

P

P

H

H

K

K

I

I

Q

Q

G

G

I

I

G

G

A

A

G

G

F

F

V

V

P

P

E

K

V

N

L

L

N

D

R

L

E

A

A

I

I

V

D

D

E

E

I

Y

F

I

T

A

V

I

R

S

N

S

E

E

A

A

L

I

E

E

T

T

A

S

R

K

R

Q

L

L

A

A

R

L

E

Q

E

E

G

G

L

L

V

V

G

G

I

I

S

S

A

S

G

G

A

A

F

A

A

A

L

I

E

Q

V

V

A

A

R

K

R

R

P

P

E

E

N

N

K

A

G

G

K

K

R

L

I

I

V

A

V

V

L

F

P

P

D

S

T

F

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

V

Q

L

L

F

F

Q

Q

D

S

Sites not aligning to the query:

61 modified: N-acetylalanine

7n2tA O-acetylserine sulfhydrylase from citrullus vulgaris in the internal aldimine state, with citrate bound (see paper)
60% identity, 99% coverage: 2:308/309 of query aligns to 1:308/309 of 7n2tA

query
sites
7n2tA

A

S

Q

T

I

I

Y

A

E

K

N

D

L

V

T

T

D

E

L

L

I

I

G

G

K

N

T

T

P

P

L

L

L

V

R

Y

L

L

R

N

K

R

V

V

A

V

A

D

G

G

I

C

E

V

A

A

D

R

I

V

V

A

A

A

K

K

L

L

E

E

S

M

F

M

N

E

P

P

G

C

G

S

S

S

V

V

K

K

D

D

R

R

I

I

G

G

V

Y

S

S

M

M

I

I

R

S

D

D

A

A

E

E

G

N

R

K

G

G

L

L

I

I

N

T

K

P

-

G

D

E

T

S

V

V

I

L

I

I

E

E

P

P

T

T

S

S

G

G

N

N

T

T

G

G

I

I

A

G

L

L

A

A

F

F

V

I

C

A

A

A

R

K

G

G

Y

Y

R

R

L

L

V

I

L

I

T

C

M

M

P

P

D

A

T

S

M

M

S

S

V

L

E

E

R

R

N

T

L

I

L

L

K

R

A

A

Y

F

G

G

A

A

E

E

L

L

V

V

L

L

T

T

R

D

G

P

A

A

E

R

G

G

M

M

K

K

G

G

A

A

I

V

N

Q

K

K

A

A

E

E

L

I

A

K

A

A

D

K

I

T

P

P

N

N

S

S

F

Y

I

I

P

L

Q

Q

F

F

R

E

N

N

P

P

A

A

N

N

P

P

H

K

I

I

H

H

R

Y

F

E

T

T

A

G

E

P

E

E

I

I

W

W

T

R

D

G

T

S

D

G

G

G

K

K

V

I

D

D

V

A

F

L

V

V

A

S

G

G

V

I

G

G

T

T

G

G

T

T

V

V

T

T

G

G

V

A

G

G

E

K

V

Y

L

L

K

K

K

E

R

Q

N

N

P

P

G

N

V

I

K

K

I

L

I

Y

A

G

V

V

E

E

P

P

F

V

D

E

S

S

P

A

V

I

L

L

S

S

G

G

H

K

P

P

A

G

P

P

H

H

K

K

I

I

Q

Q

G

G

I

I

G

G

A

A

G

G

F

F

V

I

P

P

E

G

V

V

L

L

N

D

R

V

E

N

A

L

I

L

D

D

E

E

I

V

F

I

T

Q

V

V

R

S

N

S

E

E

A

S

L

I

E

E

T

T

A

A

R

K

R

L

L

L

A

A

R

L

E

K

E

E

G

G

L

L

V

V

G

G

I

I

S

S

A

S

G

G

A

A

F

A

A

A

L

I

E
x
R

V

I

A

A

R

K

R
|
R

P

P

E

E

N

N

K

A

G

G

K

K

R

L

I

I

V

V

V

A

V

V

L

F

P

P

D

S

T

F

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

V

V

L

L

F

F

Q

E

D

S

binding phosphate ion: R277 (≠ E277), R281 (= R281)

2isqA Crystal structure of o-acetylserine sulfhydrylase from arabidopsis thaliana in complex with c-terminal peptide from arabidopsis serine acetyltransferase (see paper)
59% identity, 99% coverage: 2:306/309 of query aligns to 1:306/320 of 2isqA

query
sites
2isqA

A

S

Q

R

I

I

Y

A

E

K

N

D

L

V

T

T

D

E

L

L

I

I

G

G

K

N

T

T

P

P

L

L

L

V

R

Y

L

L

R

N

K

N

V

V

A

A

A

E

G

G

I

C

E

V

A

G

D

R

I

V

V

A

A

A

K

K

L

L

E

E

S

M

F

M

N

E

P

P

G

C

G

S

S

S

V

V

K
|
K

D

D

R

R

I

I

G

G

V

F

S

S

M

M

I

I

R

S

D

D

A

A

E

E

G

K

R

K

G

G

L

L

I

I

N

K

K

P

-

G

D

E

T

S

V

V

I

L

I

I

E

E

P

P

T
|
T

S
|
S

G
|
G

N
|
N

T
|
T

G

G

I

V

A

G

L

L

A

A

F

F

V

T

C

A

A

A

R

K

G

G

Y

Y

R

K

L

L

V

I

L

I

T

T

M

M

P

P

D

A

T

S

M

M

S

S

V

T

E

E

R

R

N

I

L

I

L

L

K

L

A

A

Y

F

G

G

A

V

E

E

L

L

V

V

L

L

T

T

R

D

G

P

A

A

E

K

G
|
G

M
|
M

K
|
K

G

G

A

A

I

I

N

A

K

K

A

A

E

E

L

I

A

L

A

A

D

K

I

T

P

P

N

N

S

G

F

Y

I

M

P

L

Q

Q

F

F

R

E

N

N

P

P

A

A

N

N

P

P

H

K

I

I

H

H

R

Y

F

E

T

T

A

G

E

P

E

E

I

I

W

W

T

K

D

G

T

T

D

G

G

G

K

K

V

I

D

D

V

G

F

F

V

V

A

S

G
|
G

V

I

G
|
G

T
|
T

G
|
G

G

G

T
|
T

V

I

T

T

G

G

V

A

G

G

E

K

V

Y

L

L

K

K

K

E

R

Q

N

N

P

A

G

N

V

V

K

K

I

L

I

Y

A

G

V

V

E

E

P

P

F

V

D

E

S

S

P

A

V

I

L

L

S

S

G

G

H

K

P

P

A
x
G

P
|
P

H
|
H

K

K

I

I

Q
|
Q

G
|
G

I

I

G

G

A

A

G

G

F

F

V

I

P

P

E

S

V

V

L

L

N

N

R

V

E

D

A

L

I

I

D

D

E

E

I

V

F

V

T

Q

V

V

R

S

N

S

E

D

E

E

A

S

L

I

E

D

T

M

A

A

R

R

R

Q

L

L

A

A

R

L

E

K

E

E

G

G

L

L

V

V

G

G

I

I

S
|
S

A

S

G

G

A

A

F

A

A

A

L

I

E

K

V

L

A

A

R

Q

R

R

P

P

E

E

N

N

K

A

G

G

K

K

R

L

I

F

V

V

V

A

V

I

L

F

P
|
P

D

S

T

F

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

V

V

L

L

F

F

active site: K44 (= K45), S267 (= S267)
binding pyridoxal-5'-phosphate: K44 (= K45), N75 (= N75), G177 (= G177), G179 (= G179), T180 (= T180), G181 (= G181), T183 (= T183), G223 (= G223), S267 (= S267), P294 (= P294)
binding : T72 (= T72), S73 (= S73), G74 (= G74), T76 (= T76), G122 (= G122), M123 (= M123), K124 (= K124), G217 (≠ A217), P218 (= P218), H219 (= H219), Q222 (= Q222), G223 (= G223)

P47998 Cysteine synthase 1; At.OAS.5-8; Beta-substituted Ala synthase 1;1; ARAth-Bsas1;1; CSase A; AtCS-A; Cys-3A; O-acetylserine (thiol)-lyase 1; OAS-TL A; O-acetylserine sulfhydrylase; Protein ONSET OF LEAF DEATH 3; EC 2.5.1.47 from Arabidopsis thaliana (Mouse-ear cress) (see 3 papers)
59% identity, 99% coverage: 2:306/309 of query aligns to 3:308/322 of P47998

query
sites
P47998

A

S

Q

R

I

I

Y

A

E

K

N

D

L

V

T

T

D

E

L

L

I

I

G

G

K

N

T

T

P

P

L

L

L

V

R

Y

L

L

R

N

K

N

V

V

A

A

A

E

G

G

I

C

E

V

A

G

D

R

I

V

V

A

A

A

K

K

L

L

E

E

S

M

F

M

N

E

P

P

G

C

G

S

S

S

V

V

K
|
K

D

D

R

R

I

I

G

G

V

F

S

S

M

M

I

I

R

S

D

D

A

A

E

E

G

K

R

K

G

G

L

L

I

I

N

K

K

P

-

G

D

E

T

S

V

V

I

L

I

I

E

E

P

P

T
|
T

S
|
S

G

G

N
|
N

T
|
T

G

G

I

V

A

G

L

L

A

A

F

F

V

T

C

A

A

A

R

K

G

G

Y

Y

R

K

L

L

V

I

L

I

T

T

M

M

P

P

D

A

T

S

M

M

S

S

V

T

E

E

R

R

N

I

L

I

L

L

K

L

A

A

Y

F

G

G

A

V

E

E

L

L

V

V

L

L

T

T

R

D

G

P

A

A

E

K

G

G

M

M

K

K

G

G

A

A

I

I

N

A

K

K

A

A

E

E

L

I

A

L

A

A

D

K

I

T

P

P

N

N

S

G

F

Y

I

M

P

L

Q

Q

Q
|
Q

F

F

R

E

N

N

P

P

A

A

N

N

P

P

H

K

I

I

H
|
H

R

Y

F

E

T

T

A
x
G

E

P

E

E

I

I

W

W

T

K

D

G

T

T

D

G

G

G

K

K

V

I

D

D

V

G

F

F

V

V

A

S

G

G

V

I

G
|
G

T
|
T

G
|
G

T
|
T

V

I

T

T

G

G

V

A

G

G

E

K

V

Y

L

L

K

K

K

E

R

Q

N

N

P

A

G

N

V

V

K

K

I

L

I

Y

A

G

V

V

E

E

P

P

F

V

D

E

S

S

P

A

V

I

L

L

S

S

G

G

H
x
K

P

P

A

G

P

P

H
|
H

K
|
K

I

I

Q

Q

G

G

I

I

G

G

A

A

G

G

F

F

V

I

P

P

E

S

V

V

L

L

N

N

R

V

E

D

A

L

I

I

D

D

E

E

I

V

F

V

T

Q

V

V

R

S

N

S

E

D

E

E

A

S

L

I

E

D

T

M

A

A

R

R

R

Q

L

L

A

A

R

L

E

K

E

E

G

G

L

L

V

V

G

G

I

I

S
|
S

A

S

G

G

A

A

F

A

A

A

L

I

E

K

V

L

A

A

R

Q

R

R

P

P

E

E

N

N

K

A

G

G

K

K

R

L

I

F

V

V

V

A

V

I

L

F

P

P

D

S

T

F

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

V

V

L

L

F

F

K46 (= K45) modified: N6-(pyridoxal phosphate)lysine; mutation to A: No cysteine synthase activity.
T74 (= T72) mutation to A: Strong reduction of cysteine synthase activity.; mutation to S: Reduction of cysteine synthase activity.
S75 (= S73) mutation S->A,N,T: Strong reduction of cysteine synthase activity.
N77 (= N75) binding pyridoxal 5'-phosphate; mutation to A: Reduction of cysteine synthase activity.; mutation to D: Strong reduction of cysteine synthase activity.
T78 (= T76) mutation T->A,S: Reduction of cysteine synthase activity.
Q147 (= Q145) mutation Q->A,E: Strong reduction of cysteine synthase activity.
H157 (= H155) mutation H->Q,N: Slight reduction of cysteine synthase activity.
G162 (≠ A160) mutation to E: In old3-1; displays a early leaf death phenotype. Abolishes cysteine synthase activity.
GTGGT 181:185 (= GTGGT 179:183) binding pyridoxal 5'-phosphate
T182 (= T180) mutation T->A,S: Slight reduction of cysteine synthase activity.
T185 (= T183) mutation T->A,S: Strong reduction of cysteine synthase activity.
K217 (≠ H215) mutation to A: Impaired interaction with SAT1.
H221 (= H219) mutation to A: Impaired interaction with SAT1.
K222 (= K220) mutation to A: Impaired interaction with SAT1.
S269 (= S267) binding pyridoxal 5'-phosphate; mutation to A: Strong reduction of cysteine synthase activity.; mutation to T: Reduction of cysteine synthase activity.

1z7yA Crystal structure of the arabidopsis thaliana o-acetylserine sulfhydrylase k46a mutant (see paper)
59% identity, 99% coverage: 2:306/309 of query aligns to 1:306/320 of 1z7yA

query
sites
1z7yA

A

S

Q

R

I

I

Y

A

E

K

N

D

L

V

T

T

D

E

L

L

I

I

G

G

K

N

T

T

P

P

L

L

L

V

R

Y

L

L

R

N

K

N

V

V

A

A

A

E

G

G

I

C

E

V

A

G

D

R

I

V

V

A

A

A

K

K

L

L

E

E

S

M

F

M

N

E

P

P

G

C

G

S

S

S

V

V

K
x
A

D

D

R

R

I

I

G

G

V

F

S

S

M

M

I

I

R

S

D

D

A

A

E

E

G

K

R

K

G

G

L

L

I

I

N

K

K

P

-

G

D

E

T

S

V

V

I

L

I

I

E

E

P

P

T

T

S

S

G
|
G

N
|
N

T
|
T

G

G

I

V

A

G

L

L

A

A

F

F

V

T

C

A

A

A

R

K

G

G

Y

Y

R

K

L

L

V

I

L

I

T

T

M

M

P

P

D

A

T

S

M

M

S

S

V

T

E

E

R

R

N

I

L

I

L

L

K

L

A

A

Y

F

G

G

A

V

E

E

L

L

V

V

L

L

T

T

R

D

G

P

A

A

E

K

G

G

M

M

K

K

G

G

A

A

I

I

N

A

K

K

A

A

E

E

L

I

A

L

A

A

D

K

I

T

P

P

N

N

S

G

F

Y

I

M

P

L

Q

Q

Q
|
Q

F

F

R

E

N

N

P

P

A

A

N

N

P

P

H

K

I

I

H

H

R

Y

F

E

T

T

A

G

E

P

E

E

I

I

W

W

T

K

D

G

T

T

D

G

G

G

K

K

V

I

D

D

V

G

F

F

V

V

A

S

G

G

V
x
I

G
|
G

T
|
T

G
|
G

G

G

T
|
T

V

I

T

T

G

G

V

A

G

G

E

K

V

Y

L

L

K

K

K

E

R

Q

N

N

P

A

G

N

V

V

K

K

I

L

I

Y

A

G

V

V

E

E

P

P

F

V

D

E

S

S

P

A

V

I

L

L

S

S

G

G

H

K

P

P

A

G

P

P

H

H

K

K

I

I

Q

Q

G
|
G

I

I

G

G

A

A

G

G

F

F

V

I

P

P

E

S

V

V

L

L

N

N

R

V

E

D

A

L

I

I

D

D

E

E

I

V

F

V

T

Q

V

V

R

S

N

S

E

D

E

E

A

S

L

I

E

D

T

M

A

A

R

R

R

Q

L

L

A

A

R

L

E

K

E

E

G

G

L

L

V

V

G

G

I

I

S
|
S

A

S

G

G

A

A

F

A

A

A

L

I

E

K

V

L

A

A

R

Q

R

R

P

P

E

E

N

N

K

A

G

G

K

K

R

L

I

F

V

V

V

A

V

I

L

F

P
|
P

D
x
S

T

F

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

V

V

L

L

F

F

active site: A44 (≠ K45), S267 (= S267)
binding n-[(3-hydroxy-2-methyl-5-{[(trihydroxyphosphoranyl)oxy]methyl}pyridin-4-yl)methylene]methionine: G74 (= G74), N75 (= N75), T76 (= T76), Q145 (= Q145), I178 (≠ V178), G179 (= G179), T180 (= T180), G181 (= G181), T183 (= T183), G223 (= G223), S267 (= S267), P294 (= P294), S295 (≠ D295)

P0A1E3 Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine sulfhydrylase A; EC 2.5.1.47 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 4 papers)
57% identity, 100% coverage: 1:308/309 of query aligns to 1:314/323 of P0A1E3

query
sites
P0A1E3

M
|
M

A

S

Q

K

I

I

Y

Y

E

E

N

D

L

N

T

S

D

L

L

T

I

I

G

G

K

H

T

T

P

P

L

L

L

V

R

R

L

L

R

N

K

R

V

I

A

G

A

N

G

G

I

-

E

-

A

-

D

R

I

I

V

L

A

A

K

K

L

V

E

E

S

S

F

R

N

N

P

P

G

S

G

F

S

S

V

V

K

K

D

C

R

R

I

I

G

G

V

A

S

N

M

M

I

I

R

W

D

D

A

A

E

E

G

K

R

R

G

G

L

V

I

L

N

K

K

P

D

G

T

V

V

E

I

L

I

V

E

E

P

P

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

A

A

L

L

A

A

F

Y

V

V

C

A

A

A

R

R

G

G

Y

Y

R

K

L

L

V

T

L

L

T

T

M

M

P

P

D

E

T

T

M

M

S

S

V

I

E

E

R

R

N

K

L

L

K

K

A

A

Y

L

G

G

A

A

E

N

L

L

V

V

L

L

T

T

R

E

G

G

A

A

E

K

G

G

M

M

K

K

G

G

A

A

I

I

N

Q

K

K

A

A

E

E

L

I

A

V

A

A

D

S

I

D

P

P

N

Q

S

K

F

Y

-

L

I

L

P

L

Q

Q

F

F

R

S

N

N

P

P

A

A

N

N

P

P

H

E

I

I

H

H

R

E

F

K

T

T

A

G

E

P

E

E

I

I

W

W

T

E

D

D

T

T

D

D

G

G

K

Q

V

V

D

D

V

V

F

F

V

I

A

S

G

G

V

V

G

G

T

T

G

G

T

T

V

L

T

T

G

G

V

V

G

T

E

R

V

Y

L

I

K

K

-

G

-

T

K

K

R

G

N

K

P

T

G

D

V

L

K

I

I

T

I

V

A

A

V

V

E

E

P

P

F

T

D

D

S

S

P

P

V

V

L

I

S

A

G

Q

G

A

-

L

-

A

-

G

-

E

-

I

H

K

P

P

A

G

P

P

H

H

K

K

I

I

Q

Q

G

G

I

I

G

G

A

A

G

G

F

F

V

I

P

P

E

G

V

N

L

L

N

D

R

L

E

K

A

L

I

I

D

D

E

K

I

V

F

V

T

G

V

I

R

T

N

N

E

E

A

A

L

I

E

S

T

T

A

A

R

R

L

L

A

M

R

E

E

E

G

G

L

I

L

L

V

A

G

G

I

I

S
|
S

A

S

G

G

A

A

A

V

F

A

A

A

L

L

E

K

V

L

A

Q

R

E

R

D

P

E

E

S

N

F

K

T

G

N

K

K

R

N

I

I

V

V

V

I

L

L

P

P

D

S

T

S

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

V

A

L

L

F

F

Q

A

D

D

M1 (= M1) modified: Initiator methionine, Removed
N72 (= N75) binding pyridoxal 5'-phosphate
S273 (= S267) binding pyridoxal 5'-phosphate

6z4nAAA structure of oass complexed with upar inhibitor (see paper)
57% identity, 100% coverage: 1:308/309 of query aligns to 2:315/321 of 6z4nAAA

query
sites
6z4nAAA

M

M

A

S

Q

K

I

I

Y

Y

E

E

N

D

L

N

T

S

D

L

L

T

I

I

G

G

K

H

T

T

P

P

L

L

L

V

R

R

L

L

R

N

K

R

V

I

A

G

A

N

G

G

I

-

E

-

A

-

D

R

I

I

V

L

A

A

K

K

L

V

E

E

S

S

F

R

N

N

P

P

G

S

G

F

S

S

V

V

K
|
K

D

C

R

R

I

I

G

G

V

A

S

N

M

M

I

I

R

W

D

D

A

A

E

E

G

K

R

R

G

G

L

V

I

L

N

K

K

P

D

G

T

V

V

E

I

L

I

V

E

E

P

P

T
|
T

S

S

G
|
G

N
|
N

T
|
T

G

G

I

I

A

A

L

L

A

A

F

Y

V

V

C

A

A

A

R

R

G

G

Y

Y

R

K

L

L

V

T

L

L

T

T

M

M

P

P

D

E

T

T

M

M

S

S

V

I

E

E

R

R

N

K

L

L

K

K

A

A

Y

L

G

G

A

A

E

N

L

L

V

V

L

L

T

T

R

E

G

G

A

A

E

K

G

G

M

M

K

K

G

G

A

A

I

I

N

Q

K

K

A

A

E

E

L

I

A

V

A

A

D

S

I

D

P

P

N

Q

S

K

F

Y

-

L

I

L

P

L

Q

Q

Q
|
Q

F
|
F

R

S

N

N

P

P

A

A

N

N

P

P

H

E

I

I

H

H

R

E

F

K

T

T

A

G

E

P

E

E

I

I

W

W

T

E

D

D

T

T

D

D

G

G

K

Q

V

V

D

D

V

V

F

F

V

I

A

S

G

G

V
|
V

G
|
G

T
|
T

G
|
G

G

G

T
|
T

V

L

T

T

G

G

V

V

G

T

E

R

V

Y

L

I

K

K

-

G

-

T

K

K

R

G

N

K

P

T

G

D

V

L

K

I

I

T

I

V

A

A

V

V

E

E

P

P

F

T

D

D

S

S

P

P

V

V

L

I

S

A

G

Q

G

A

-

L

-

A

-

G

-

E

-

I

H

K

P

P

A

G

P

P

H

H

K

K

I

I

Q
|
Q

G
|
G

I
|
I

G

G

A
|
A

G

G

F

F

V

I

P

P

E

G

V

N

L

L

N

D

R

L

E

K

A

L

I

I

D

D

E

K

I

V

F

V

T

G

V

I

R

T

N

N

E

E

A

A

L

I

E

S

T

T

A

A

R

R

L

L

A

M

R

E

E

E

G

G

L

I

L

L

V

A

G

G

I

I

S
|
S

A

S

G

G

A

A

A

V

F

A

A

A

L

L

E

K

V

L

A

Q

R

E

R

D

P

E

E

S

N

F

K

T

G

N

K

K

R

N

I

I

V

V

V

I

L

L

P
|
P

D

S

T

S

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

V

A

L

L

F

F

Q

A

D

D

binding pyridoxal-5'-phosphate: K43 (= K45), N73 (= N75), V177 (= V178), G178 (= G179), T179 (= T180), G180 (= G181), T182 (= T183), G230 (= G223), S274 (= S267), P301 (= P294)
binding (1~{S},2~{S})-1-[(4-methylphenyl)methyl]-2-phenyl-cyclopropane-1-carboxylic acid: K43 (= K45), T70 (= T72), G72 (= G74), N73 (= N75), T74 (= T76), Q144 (= Q145), F145 (= F146), Q229 (= Q222), G230 (= G223), I231 (= I224), A233 (= A226)

P0ABK5 Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine sulfhydrylase A; S-carboxymethylcysteine synthase; Sulfate starvation-induced protein 5; SSI5; EC 2.5.1.47; EC 4.5.1.5 from Escherichia coli (strain K12) (see 5 papers)
57% identity, 100% coverage: 1:308/309 of query aligns to 1:314/323 of P0ABK5

query
sites
P0ABK5

M
|
M

A

S

Q

K

I

I

Y

F

E

E

N

D

L

N

T

S

D

L

L

T

I

I

G

G

K

H

T

T

P

P

L

L

L

V

R

R

L

L

R

N

K

R

V

I

A

G

A

N

G

G

I

-

E

-

A

-

D

R

I

I

V

L

A

A

K

K

L

V

E

E

S

S

F

R

N

N

P

P

G

S

G

F

S

S

V

V

K
|
K

D

C

R

R

I

I

G

G

V

A

S

N

M

M

I

I

R

W

D

D

A

A

E

E

G

K

R

R

G

G

L

V

I

L

N

K

K

P

D

G

T

V

V

E

I

L

I

V

E

E

P

P

T

T

S

S

G

G

N

N

T

T

G

G

I

I

A

A

L

L

A

A

F

Y

V

V

C

A

A

A

R

R

G

G

Y

Y

R

K

L

L

V

T

L

L

T

T

M

M

P

P

D

E

T

T

M

M

S

S

V

I

E

E

R

R

N

K

L

L

K

K

A

A

Y

L

G

G

A

A

E

N

L

L

V

V

L

L

T

T

R

E

G

G

A

A

E

K

G

G

M

M

K

K

G

G

A

A

I

I

N

Q

K

K

A

A

E

E

L

I

A

V

A

A

D

S

I

N

P

P

N

E

S

K

F

Y

-

L

I

L

P

L

Q

Q

F

F

R

S

N

N

P

P

A

A

N

N

P

P

H

E

I

I

H

H

R

E

F

K

T

T

A

G

E

P

E

E

I

I

W

W

T

E

D

D

T

T

D

D

G

G

K

Q

V

V

D

D

V

V

F

F

V

I

A

A

G

G

V

V

G

G

T

T

G

G

T

T

V

L

T

T

G

G

V

V

G

S

E

R

V

Y

L

I

K

K

-

G

-

T

K

K

R

G

N

K

P

T

G

D

V

L

K

I

I

S

I

V

A

A

V

V

E

E

P

P

F

T

D

D

S

S

P

P

V

V

L

I

S

A

G

Q

G

A

-

L

-

A

-

G

-

E

-

I

H

K

P

P

A

G

P

P

H

H

K

K

I

I

Q

Q

G

G

I

I

G

G

A

A

G

G

F

F

V

I

P

P

E

A

V

N

L

L

N

D

R

L

E

K

A

L

I

V

D

D

E

K

I

V

F

I

T

G

V

I

R

T

N

N

E

E

A

A

L

I

E

S

T

T

A

A

R

R

L

L

A

M

R

E

E

E

G

G

L

I

L

L

V

A

G

G

I

I

S

S

A

S

G

G

A

A

A

V

F

A

A

A

L

L

E

K

V

L

A

Q

R

E

R

D

P

E

E

S

N

F

K

T

G

N

K

K

R

N

I

I

V

V

V

I

L

L

P

P

D

S

T

S

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

V

A

L

L

F

F

Q

A

D

D

M1 (= M1) modified: Initiator methionine, Removed
K42 (= K45) modified: N6-(pyridoxal phosphate)lysine; mutation to A: Still stimulates tRNase activity of CdiA-CT in vitro and in vivo.

1d6sA Crystal structure of the k41a mutant of o-acetylserine sulfhydrylase complexed in external aldimine linkage with methionine (see paper)
57% identity, 99% coverage: 2:308/309 of query aligns to 1:313/322 of 1d6sA

query
sites
1d6sA

A

S

Q

K

I

I

Y

Y

E

E

N

D

L

N

T

S

D

L

L

T

I

I

G

G

K

H

T

T

P

P

L

L

L

V

R

R

L

L

R

N

K

R

V

I

A

G

A

N

G

G

I

-

E

-

A

-

D

R

I

I

V

L

A

A

K

K

L

V

E

E

S

S

F

R

N

N

P

P

G

S

G

F

S

S

V

V

K
x
A

D

C

R

R

I

I

G

G

V

A

S

N

M

M

I

I

R

W

D

D

A

A

E

E

G

K

R

R

G

G

L

V

I

L

N

K

K

P

D

G

T

V

V

E

I

L

I

V

E

E

P

P

T
|
T

S
x
N

G

G

N
|
N

T
|
T

G

G

I

I

A

A

L

L

A

A

F

Y

V

V

C

A

A

A

R

R

G

G

Y

Y

R

K

L

L

V

T

L

L

T

T

M

M

P

P

D

E

T

T

M

M

S

S

V

I

E

E

R

R

N

K

L

L

K

K

A

A

Y

L

G

G

A

A

E

N

L

L

V

V

L

L

T

T

R

E

G

G

A

A

E

K

G

G

M

M

K

K

G

G

A

A

I

I

N

Q

K

K

A

A

E

E

L

I

A

V

A

A

D

S

I

D

P

P

N

Q

S

K

F

Y

-

L

I

L

P

L

Q

Q

Q
|
Q

F
|
F

R

S

N

N

P

P

A

A

N

N

P

P

H

E

I

I

H

H

R

E

F

K

T

T

A

G

E

P

E

E

I

I

W

W

T

E

D

D

T

T

D

D

G

G

K

Q

V

V

D

D

V

V

F

F

V

I

A

S

G

G

V

V

G
|
G

T
|
T

G
|
G

G

G

T
|
T

V

L

T

T

G

G

V

V

G

T

E

R

V

Y

L

I

K

K

-

G

-

T

K

K

R

G

N

K

P

T

G

D

V

L

K

I

I

T

I

V

A

A

V

V

E

E

P

P

F

T

D

D

S

S

P

P

V

V

L

I

S

A

G

Q

G

A

-

L

-

A

-

G

-

E

-

I

H

K

P

P

A

G

P

P

H

H

K

K

I

I

Q

Q

G
|
G

I

I

G

G

A

A

G

G

F

F

V

I

P

P

E

G

V

N

L

L

N

D

R

L

E

K

A

L

I

I

D

D

E

K

I

V

F

V

T

G

V

I

R

T

N

N

E

E

A

A

L

I

E

S

T

T

A

A

R

R

L

L

A

M

R

E

E

E

G

G

L

I

L

L

V

A

G

G

I

I

S
|
S

A

S

G

G

A

A

A

V

F

A

A

A

L

L

E

K

V

L

A

Q

R

E

R

D

P

E

E

S

N

F

K

T

G

N

K

K

R

N

I

I

V

V

V

I

L

L

P
|
P

D

S

T

S

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

V

A

L

L

F

F

Q

A

D

D

active site: A41 (≠ K45), G228 (= G223)
binding methionine: T68 (= T72), N69 (≠ S73), N71 (= N75), T72 (= T76), Q142 (= Q145), F143 (= F146), G176 (= G179), G228 (= G223)
binding pyridoxal-5'-phosphate: N71 (= N75), G176 (= G179), T177 (= T180), G178 (= G181), T180 (= T183), G228 (= G223), S272 (= S267), P299 (= P294)

3vbeC Crystal structure of beta-cyanoalanine synthase in soybean (see paper)
53% identity, 99% coverage: 4:308/309 of query aligns to 11:316/329 of 3vbeC

query
sites
3vbeC

I

I

Y

K

E

K

N

H

L

V

T

S

D

Q

L

L

I

I

G

G

K

R

T

T

P

P

L

L

L

V

R

Y

L

L

R

N

K

K

V

V

A

T

A

E

G

G

I

C

E

G

A

A

D

Y

I

V

V

A

A

V

K

K

L

Q

E

E

S

M

F

M

N

Q

P

P

G

T

G

A

S

S

V

I

K
|
K

D

D

R

R

I

P

G

A

V

Y

S

A

M

M

I

I

R

T

D

D

A

A

E

E

G

E

R

K

G

N

L

L

I

I

N

T

K

P

-

G

D

K

T

T

V

T

I

L

I

I

E

E

P

P

T

T

S
|
S

G

G

N
|
N

T

M

G

G

I

I

A

S

L

M

A

A

F

F

V

M

C

A

A

A

A

M

R

K

G

G

Y

Y

R

K

L

M

V

V

L

L

T

T

M

M

P

P

D

S

T

Y

M

T

S

S

V

L

E

E

R

R

N

V

L

T

L

M

K

R

A

A

Y

F

G

G

A

A

E

E

L

L

V

I

L

L

T

T

R

D

G

P

A

A

E

K

G

G

M

M

K

G

G

G

A

T

I

V

N

K

K

K

A

A

E

Y

E

E

L

L

A

L

A

E

D

N

I

T

P

P

N

N

S

A

F

H

I

M

P

L

Q

Q

F

F

R

S

N

N

P

P

A

A

N

N

P

T

H

Q

I

V

H

H

R

F

F

E

T

T

A

G

E

P

E

E

I

I

W

W

T

E

D

D

T

T

D

N

G

G

K

Q

V

V

D

D

V

I

F

F

V

V

A
x
M

G

G

V

I

G
|
G

T
x
S

G
|
G

G

G

T
|
T

V

V

T

S

G

G

V

V

G

G

E

Q

V

Y

L

L

K

K

K

S

R

K

N

N

P

P

G

N

V

V

K

K

I

I

I

Y

A

G

V

V

E
|
E

P

P

F

S

D

E

S
|
S

P

N

V

V

L

L

S

N

G

G

H

K

P

P

A

G

P

P

H

H

K

H

I

I

Q

T

G
|
G

I

N

G

G

A

V

G

G

F

F

V

K

P

P

E

D

V

I

L

L

N

D

R

L

E

D

A

V

I

M

D

E

E

K

I

V

F

L

T

E

V

V

R

S

N

S

E

E

E

D

A

A

L

V

E

N

T

M

A

A

R

R

R

V

L

L

A

A

R

L

E

K

E

E

G

G

L

L

L

M

V

V

G

G

I

I

S
|
S

A

S

G

G

A

A

A

N

A

T

F

V

A

A

L

L

E

R

V

L

A

A

R

Q

R

L

P

P

E

E

N

N

K

K

G

G

K

K

R

L

I

I

V

V

V

T

V

V

L

H

P
|
P

D

S

T

F

G

G

E

E

R

R

Y

Y

L

L

S

S

T

S

V

V

L

L

F

F

Q

Q

D

E

active site: K52 (= K45), S81 (= S73), E212 (= E204), S216 (= S208), S275 (= S267), P302 (= P294)
binding pyridoxal-5'-phosphate: K52 (= K45), N83 (= N75), M184 (≠ A176), G187 (= G179), S188 (≠ T180), G189 (= G181), T191 (= T183), G231 (= G223), S275 (= S267), P302 (= P294)

3vc3A Crystal structure of beta-cyanoalanine synthase k95a mutant in soybean (see paper)
52% identity, 99% coverage: 4:308/309 of query aligns to 4:309/322 of 3vc3A

query
sites
3vc3A

I

I

Y

K

E

K

N

H

L

V

T

S

D

Q

L

L

I

I

G

G

K

R

T

T

P

P

L

L

L

V

R

Y

L

L

R

N

K

K

V

V

A

T

A

E

G

G

I

C

E

G

A

A

D

Y

I

V

V

A

A

V

K

K

L

Q

E

E

S

M

F

M

N

Q

P

P

G

T

G

A

S

S

V

I

K
x
A

D

D

R

R

I

P

G

A

V

Y

S

A

M

M

I

I

R

T

D

D

A

A

E

E

G

E

R

K

G

N

L

L

I

I

N

T

K

P

-

G

D

K

T

T

V

T

I

L

I

I

E

E

P

P

T
|
T

S
|
S

G

G

N
|
N

T
x
M

G

G

I

I

A

S

L

M

A

A

F

F

V

M

C

A

A

A

A

M

R

K

G

G

Y

Y

R

K

L

M

V

V

L

L

T

T

M

M

P

P

D

S

T

Y

M

T

S

S

V

L

E

E

R

R

N

V

L

T

L

M

K

R

A

A

Y

F

G

G

A

A

E

E

L

L

V

I

L

L

T

T

R

D

G

P

A

A

E

K

G

G

M

M

K

G

G

G

A

T

I

V

N

K

K

K

A

A

E

Y

E

E

L

L

A

L

A

E

D

N

I

T

P

P

N

N

S

A

F

H

I

M

P

L

Q

Q

Q
|
Q

F

F

R

S

N

N

P

P

A

A

N

N

P

T

H

Q

I

V

H

H

R

F

F

E

T

T

A

G

E

P

E

E

I

I

W

W

T

E

D

D

T

T

D

N

G

G

K

Q

V

V

D

D

V

I

F

F

V

V

A
x
M

G

G

V

I

G
|
G

T
x
S

G
|
G

G

G

T
|
T

V

V

T

S

G

G

V

V

G

G

E

Q

V

Y

L

L

K

K

K

S

R

K

N

N

P

P

G

N

V

V

K

K

I

I

I

Y

A

G

V

V

E

E

P

P

F

S

D

E

S

S

P

N

V

V

L

L

S

N

G

G

H

K

P

P

A

G

P

P

H

H

K

H

I

I

Q

T

G
|
G

I

N

G

G

A

V

G

G

F

F

V

K

P

P

E

D

V

I

L

L

N

D

R

L

E

D

A

V

I

M

D

E

E

K

I

V

F

L

T

E

V

V

R

S

N

S

E

E

E

D

A

A

L

V

E

N

T

M

A

A

R

R

R

V

L

L

A

A

R

L

E

K

E

E

G

G

L

L

L

M

V

V

G

G

I

I

S
|
S

A

S

G

G

A

A

A

N

A

T

F

V

A

A

L

L

E

R

V

L

A

A

R

Q

R

L

P

P

E

E

N

N

K

K

G

G

K

K

R

L

I

I

V

V

V

T

V

V

L

H

P
|
P

D

S

T

F

G

G

E

E

R

R

Y

Y

L

L

S

S

T

S

V

V

L

L

F

F

Q

Q

D

E

active site: A45 (≠ K45), S268 (= S267), P295 (= P294)
binding n-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-l-cysteine: T73 (= T72), S74 (= S73), N76 (= N75), M77 (≠ T76), Q146 (= Q145), M177 (≠ A176), G180 (= G179), S181 (≠ T180), G182 (= G181), T184 (= T183), G224 (= G223), S268 (= S267), P295 (= P294)

Q9FS29 Bifunctional L-3-cyanoalanine synthase/cysteine synthase 2, mitochondrial; EC 2.5.1.47; EC 4.4.1.9 from Solanum tuberosum (Potato) (see paper)
52% identity, 99% coverage: 3:308/309 of query aligns to 27:333/347 of Q9FS29

query
sites
Q9FS29

Q

N

I

I

Y

K

E

S

N

E

L

V

T

S

D

Q

L

L

I

I

G

G

K

K

T

T

P

P

L

M

L

V

R

Y

L

L

R

K

K

K

V

V

A

T

A

E

G

G

I

C

E

G

A

A

D

Y

I

I

V

A

A

V

K

K

L

Q

E

E

S

M

F

F

N

Q

P

P

G

T

G

S

S

S

V

I

K

K

D

D

R

R

I

P

G

A

V

L

S

A

M

M

I

I

R

N

D

D

A

A

E

E

G

K

R

K

G

G

L

L

I

I

N

S

K

P

D

E

-

K

T

T

V

T

I

L

I

I

E

E

P

P

T

T

S

S

G

G

N

N

T

M

G

G

I

I

A

S

L

M

A

A

F

F

V

M

C

A

A

A

A

M

R

K

G

G

Y

Y

R

K

L

M

V

V

L

L

T

T

M

M

P

P

D

S

T

Y

M

T

S

S

V

M

E

E

R

R

N

V

L

T

L

M

K

R

A

A

Y

F

G

G

A

A

E

D

L

L

V

I

L

L

T

T

R

D

G

P

A

T

E

K

G

G

M

M

K

G

G

G

A

T

I

V

N

K

K

K

A

A

E

Y

E
|
E

L

L

A

L

A

E

D

S

I

T

P

P

N

N

S

A

F

F

I

M

P

L

Q

Q

F

F

R

S

N

N

P

P

A

A

N

N

P

T

H

Q

I

V

H

H

R

F

F

D

T

T

A

G

E

P

E

E

I

I

W

W

T

E

D

E

T

T

D

L

G

G

K

N

V

V

D

D

V

I

F

F

V

V

A

M

G

G

V

I

G

G

T

S

G

G

T

T

V

V

T

T

G

G

V

V

G

G

E

L

V

Y

L

L

K

K

K

S

R

K

N

N

P

P

G

N

V

V

K

K

I

I

I

Y

A

G

V

L

E

E

P

P

F

T

D

E

S

S

P

N

V

I

L

L

S

N

G

G

H

K

P

P

A

G

P

P

H

H

K

H

I

I

Q

T

G

G

I

N

G

G

A

V

G

G

F

S

V

K

P

P

E

D

V

I

L

V

N

D

R

M

E

D

A

L

I

M

D

E

E

E

I

V

F

L

T

M

V

V

R

S

N

S

E

E

E

D

A

A

L

V

E

N

T

M

A

A

R

R

R

E

L

L

A

A

R

V

E

K

E

E

G

G

L

L

L

M

V

V

G

G

I

I

S

S

A

S

G

G

A

A

A

N

A

T

F

V

A

A

L

L

E

R

V

L

A

A

R

Q

R

K

P

P

E

E

N

N

K

K

G

G

K

K

R

L

I

I

V

V

V

T

V

V

L

H

P

A

D

S

T

F

G

G

E

E

R

R

Y

Y

L

L

S

S

T

S

V

V

L

L

F

Y

Q

Q

D

D

E157 (= E132) mutation E->N,Q: No effect on catalytic activities.

Query Sequence

>WP_004511507.1 NCBI__GCF_000012925.1:WP_004511507.1
MAQIYENLTDLIGKTPLLRLRKVAAGIEADIVAKLESFNPGGSVKDRIGVSMIRDAEGRG
LINKDTVIIEPTSGNTGIALAFVCAARGYRLVLTMPDTMSVERRNLLKAYGAELVLTRGA
EGMKGAINKAEELAADIPNSFIPQQFRNPANPHIHRFTTAEEIWTDTDGKVDVFVAGVGT
GGTVTGVGEVLKKRNPGVKIIAVEPFDSPVLSGGHPAPHKIQGIGAGFVPEVLNREAIDE
IFTVRNEEALETARRLAREEGLLVGISAGAAAFAALEVARRPENKGKRIVVVLPDTGERY
LSTVLFQDQ

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory