SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_004513581.1 NCBI__GCF_000012925.1:WP_004513581.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P16703 Cysteine synthase B; CSase B; O-acetylserine (thiol)-lyase B; OAS-TL B; O-acetylserine sulfhydrylase B; EC 2.5.1.47 from Escherichia coli (strain K12) (see paper)
48% identity, 95% coverage: 7:299/308 of query aligns to 3:292/303 of P16703

query
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P16703

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N71 (= N75) binding pyridoxal 5'-phosphate
S255 (= S261) binding pyridoxal 5'-phosphate

2bhtA Crystal structure of o-acetylserine sulfhydrylase b (see paper)
47% identity, 95% coverage: 7:299/308 of query aligns to 3:292/294 of 2bhtA

query
sites
2bhtA

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active site: K41 (= K45), S69 (= S73), Q199 (≠ E205), G203 (= G209), S255 (= S261), C280 (≠ P287)
binding pyridoxal-5'-phosphate: K41 (= K45), N71 (= N75), M173 (= M179), G174 (= G180), T175 (= T181), T176 (≠ G182), T178 (= T184), G208 (= G214), S255 (= S261), C280 (≠ P287)

5xoqA Crystal structure of o-acetylserine sulfhydrylase with bound transcription factor peptide inhibitor from planctomyces limnophilus
47% identity, 95% coverage: 9:302/308 of query aligns to 9:309/310 of 5xoqA

query
sites
5xoqA

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binding : T72 (= T72), S73 (= S73), G74 (= G74), T76 (= T76), M123 (≠ T123), Q144 (= Q146), R218 (vs. gap), H219 (= H210), Q222 (= Q213), G223 (= G214), A226 (≠ E220)

P9WP55 O-acetylserine sulfhydrylase; OAS sulfhydrylase; OASS; Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine-specific cysteine synthase; Sulfide-dependent cysteine synthase; EC 2.5.1.47 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
49% identity, 95% coverage: 12:303/308 of query aligns to 11:309/310 of P9WP55

query
sites
P9WP55

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K44 (= K45) modified: N6-(pyridoxal phosphate)lysine
N74 (= N75) binding pyridoxal 5'-phosphate
GTGGT 178:182 (= GTGGT 180:184) binding pyridoxal 5'-phosphate
S266 (= S261) binding pyridoxal 5'-phosphate

2q3dA 2.2 a resolution crystal structure of o-acetylserine sulfhydrylase (oass) from mycobacterium tuberculosis in complex with the reaction intermediate alpha-aminoacrylate (see paper)
49% identity, 94% coverage: 12:299/308 of query aligns to 11:305/306 of 2q3dA

query
sites
2q3dA

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N

V

P

G

A

G

N

S

S

V

V

K
|
K

D

D

R

R

P

I

A

G

L

V

Y

A

M

M

L

L

A

Q

K

A

A

A

E

E

A

Q

A

A

G

G

E

L

L

I

S

K

P

P

E

D

R

T

T

I

I

I

L

L

E

E

P

P

T
|
T

S
|
S

G

G

N
|
N

T
|
T

G

G

I

I

A

A

I

L

A

A

M

M

L

V

G

C

A

A

K

R

G

G

Y

Y

R

R

V

C

K

V

L

L

V

T

M

M

P

P

A

E

C

T

V

M

S

S

L

L

E

E

R

R

A

M

V

L

L

L

E

R

A

A

Y

Y

G

G

A

A

E

E

L

L

V

I

L

L

S

T

P

P

A

G

E

A

E

D

A

G

T

M

D

S

G

G

A

A

I

I

R

A

L

K

A

A

H

E

R

E

I

-

L

L

D

A

E

K

E

T

P

D

D

Q

R

R

Y

Y

Y

F

M

V

P

P

N

Q

Q
|
Q

Y

F

A

E

N

N

P

P

N

A

N

N

P

P

L

A

A

I

H

H

Y

R

E

V

T

T

G

A

P

E

E

E

I

V

Y

W

R

R

G

D

T

T

D

D

G

G

T

K

V

V

D

D

F

I

F

V

V

V

A

A

G

G

M
x
V

G
|
G

T
|
T

G
|
G

G

G

T
|
T

L

I

M

T

G

G

V

V

S

A

R

Q

Y

V

F

I

R

K

E

E

T

R

K

K

P

P

S

S

V

A

R

R

I

F

V

V

G

A

V

V

E

E

P

P

R

A

L

A

G

S

-

P

-

V

-

L

-

S

-

G

-

Q

-

K

-

G

-

P

H

H

K

P

V

I

Q

Q

G
|
G

L
x
I

K

G

N

-

M

-

Q

-

E

A

A

G

I

F

V

V

P

P

A

P

I

V

Y

L

N

D

P

Q

A

D

A

L

L

V

D

D

L

E

K

I

L

I

T

T

V

V

Q

G

D

N

E

E

P

D

A

A

F

L

D

N

T

V

A

A

R

R

D

R

L

L

A

A

V

R

R

E

E

E

G

G

L

L

F

L

V

V

G

G

M

I

S
|
S

S

S

G

G

A

A

M

T

A

V

G

A

A

A

I

L

R

Q

V

V

A

A

K

R

D

R

A

P

P

E

-

N

A

A

G

G

S

K

T

L

I

I

V

V

L

V

L

L

P
|
P

D
|
D

R

F

G

G

D

E

R

R

Y

Y

L

L

S

S

T

T

V

P

L

L

F

F

active site: K44 (= K45), S266 (= S261), P293 (= P287)
binding 2-[(3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl)-amino]-propionic acid: K44 (= K45), T71 (= T72), S72 (= S73), N74 (= N75), T75 (= T76), Q144 (= Q146), V177 (≠ M179), G178 (= G180), T179 (= T181), G180 (= G182), T182 (= T184), G222 (= G214), I223 (≠ L215), S266 (= S261), P293 (= P287), D294 (= D288)

3zeiA Structure of the mycobacterium tuberculosis o-acetylserine sulfhydrylase (oass) cysk1 in complex with a small molecule inhibitor (see paper)
48% identity, 92% coverage: 12:294/308 of query aligns to 11:300/300 of 3zeiA

query
sites
3zeiA

I

I

G

G

S

R

T

T

P

P

L

L

V

V

E

R

I

L

R

R

K

R

L

V

N

T

P

D

N

G

P

A

K

V

V

A

R

D

I

I

L

V

A

A

K

K

L

L

E

E

G

F

N

F

N

N

V

P

G

A

G

N

S

S

V

V

K
|
K

D

D

R

R

P

I

A

G

L

V

Y

A

M

M

L

L

A

Q

K

A

A

A

E

E

A

Q

A

A

G

G

E

L

L

I

S

K

P

P

E

D

R

T

T

I

I

I

L

L

E

E

P

P

T
|
T

S
|
S

G

G

N
|
N

T

T

G

G

I

I

A

A

I

L

A

A

M

M

L

V

G

C

A

A

K

R

G

G

Y

Y

R

R

V

C

K

V

L

L

V

T

M

M

P

P

A

E

C

T

V

M

S

S

L

L

E

E

R

R

A

M

V

L

L

L

E

R

A

A

Y

Y

G

G

A

A

E

E

L

L

V

I

L

L

S

T

P

P

A

G

E

A

E

D

A

G

T

M

D

S

G

G

A

A

I
|
I

R

A

L

K

A

A

H

E

R

E

I

-

L

L

D

A

E

K

E

T

P

D

D

Q

R

R

Y

Y

Y

F

M

V

P

P

N

Q

Q
|
Q

Y
x
F

A

E

N

N

P

P

N

A

N

N

P

P

L

A

A

I

H

H

Y

R

E

V

T

T

G

A

P

E

E

E

I

V

Y

W

R

R

G

D

T

T

D

D

G

G

T

K

V

V

D

D

F

I

F

V

V

V

A

A

G

G

M
x
V

G
|
G

T
|
T

G
|
G

G

G

T
|
T

L

I

M

T

G

G

V

V

S

A

R

Q

Y

V

F

I

R

K

E

E

T

R

K

K

P

P

S

S

V

A

R

R

I

F

V

V

G

A

V

V

E

E

P

P

R

A

L

A

G

S

-

P

-

V

-

L

-

S

-

G

-

Q

-
x
K

-

G

-

P

H

H

K

P

V

I

Q

Q

G
|
G

L

I

K

G

N

-

M

-

Q

-

E
x
A

A

G

I
x
F

V

V

P

P

A

P

I

V

Y

L

N

D

P

Q

A

D

A

L

L

V

D

D

L

E

K

I

L

I

T

T

V

V

Q

G

D

N

E

E

P

D

A

A

F

L

D

N

T

V

A

A

R

R

D

R

L

L

A

A

V

R

R

E

E

E

G

G

L

L

F

L

V

V

G

G

M

I

S
|
S

S

S

G

G

A

A

M

T

A

V

G

A

A

A

I

L

R

Q

V

V

A

A

K

R

D

R

-

P

A

E

P

N

A

A

G

G

S

K

T

L

I

I

V

V

L

V

L

L

P
|
P

D
|
D

R

F

G

G

D

E

R

R

Y

Y

L

L

active site: K44 (= K45), S266 (= S261), P293 (= P287)
binding 3-[(Z)-[(5Z)-5-[[2-(2-hydroxy-2-oxoethyloxy)phenyl]methylidene]-3-methyl-4-oxidanylidene-1,3-thiazolidin-2-ylidene]amino]benzoic acid: T71 (= T72), S72 (= S73), I126 (= I127), Q144 (= Q146), F145 (≠ Y147), K215 (vs. gap), G222 (= G214), A225 (≠ E220), F227 (≠ I222)
binding pyridoxal-5'-phosphate: K44 (= K45), N74 (= N75), V177 (≠ M179), G178 (= G180), T179 (= T181), G180 (= G182), T182 (= T184), G222 (= G214), S266 (= S261), P293 (= P287), D294 (= D288)

2q3cA 2.1 a resolution crystal structure of o-acetylserine sulfhydrylase (oass) holoenzyme from mycobacterium tuberculosis in complex with the inhibitory peptide dfsi (see paper)
48% identity, 92% coverage: 12:294/308 of query aligns to 11:300/300 of 2q3cA

query
sites
2q3cA

I

I

G

G

S

R

T

T

P

P

L

L

V

V

E

R

I

L

R

R

K

R

L

V

N

T

P

D

N

G

P

A

K

V

V

A

R

D

I

I

L

V

A

A

K

K

L

L

E

E

G

F

N

F

N

N

V

P

G

A

G

N

S

S

V

V

K
|
K

D

D

R

R

P

I

A

G

L

V

Y

A

M

M

L

L

A

Q

K

A

A

A

E

E

A

Q

A

A

G

G

E

L

L

I

S

K

P

P

E

D

R

T

T

I

I

I

L

L

E

E

P

P

T
|
T

S
|
S

G
|
G

N

N

T
|
T

G

G

I

I

A

A

I

L

A

A

M

M

L

V

G

C

A

A

K

R

G

G

Y

Y

R

R

V

C

K

V

L

L

V

T

M

M

P

P

A

E

C

T

V

M

S

S

L

L

E

E

R

R

A

M

V

L

L

L

E

R

A

A

Y

Y

G

G

A

A

E

E

L

L

V

I

L

L

S

T

P

P

A

G

E

A

E

D

A

G

T
x
M

D

S

G

G

A

A

I

I

R

A

L

K

A

A

H

E

R

E

I

-

L

L

D

A

E

K

E

T

P

D

D

Q

R

R

Y

Y

Y

F

M

V

P

P

N

Q

Q
|
Q

Y

F

A

E

N

N

P

P

N

A

N

N

P

P

L

A

A

I

H

H

Y

R

E

V

T

T

G

A

P

E

E

E

I

V

Y

W

R

R

G

D

T

T

D

D

G

G

T

K

V

V

D

D

F

I

F

V

V

V

A

A

G

G

M

V

G

G

T

T

G

G

T

T

L

I

M

T

G

G

V

V

S

A

R

Q

Y

V

F

I

R

K

E

E

T

R

K

K

P

P

S

S

V

A

R

R

I

F

V

V

G

A

V

V

E

E

P

P

R

A

L

A

G

S

-

P

-

V

-

L

-

S

-

G

-

Q

-
x
K

-

G

-

P

H

H

K

P

V

I

Q

Q

G
|
G

L

I

K

G

N

-

M

-

Q

-

E
x
A

A

G

I

F

V

V

P

P

A

P

I

V

Y

L

N

D

P

Q

A

D

A

L

L

V

D

D

L

E

K

I

L

I

T

T

V

V

Q

G

D

N

E

E

P

D

A

A

F

L

D

N

T

V

A

A

R

R

D

R

L

L

A

A

V

R

R

E

E

E

G

G

L

L

F

L

V

V

G

G

M

I

S
|
S

S

S

G

G

A

A

M

T

A

V

G

A

A

A

I

L

R

Q

V

V

A

A

K

R

D

R

-

P

A

E

P

N

A

A

G

G

S

K

T

L

I

I

V

V

L

V

L

L

P
|
P

D

D

R

F

G

G

D

E

R

R

Y

Y

L

L

active site: K44 (= K45), S266 (= S261), P293 (= P287)
binding : T71 (= T72), S72 (= S73), G73 (= G74), T75 (= T76), M122 (≠ T123), Q144 (= Q146), K215 (vs. gap), G222 (= G214), A225 (≠ E220)

4lmaA Crystal structure analysis of o-acetylserine sulfhydrylase cysk1 from microcystis aeruginosa 7806 (see paper)
46% identity, 94% coverage: 12:302/308 of query aligns to 11:311/318 of 4lmaA

query
sites
4lmaA

I

V

G

G

S

R

T

T

P

P

L

L

V

V

E

Q

I

L

R

N

K

R

L

I

N

-

P

-

N

-

P

P

K

Q

V

A

-

E

-

G

-

C

-

L

-

G

R

R

I

I

L

V

A

M

K

K

L

L

E

E

G

G

N

M

N

N

V

P

G

A

S

S

V

V

K
|
K

D

D

R

R

P

I

A

G

L

T

Y

H

M

M

L

I

A

N

K

S

A

A

E

E

A

K

A

A

G

G

E

L

L

I

S

N

P

P

E

E

R

T

T

T

I

V

L

L

-

V

E

E

P

P

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

A

A

I

L

A

A

M

M

L

T

G

A

A

A

K

K

G

G

Y

Y

R

R

V

L

K

I

L

L

V

T

M

M

P

P

A

E

C

T

V

M

S

S

L

L

E

E

R

R

A

A

V

M

L

L

E

K

A

A

Y

Y

G

G

A

A

E

T

L

L

V

E

L

L

S

T

P

P

A

G

E

S

E

Q

A

G

T

M

D

K

G

G

A

A

I

I

R

L

L

R

A

A

H

Q

R

Q

I

I

L

V

D

D

E

S

E

I

P

P

D

G

R

-

Y

A

Y

Y

M

M

P

L

N

Q

Q

Q

Y

F

A

R

N

N

P

P

N

S

N

N

P

P

L

E

A

I

H

H

Y

R

E

L

T

T

G

A

P

E

E

E

I

I

Y

W

R

Q

G

D

T

T

D

E

G

G

T

Q

V

V

D

D

F

F

F

I

V

V

A

A

G

G

M

V

G
|
G

T
|
T

G
|
G

G

G

T
|
T

L

I

M

T

G

G

V

V

S

A

R

E

Y

V

F

I

R

K

E

S

T

R

K

K

P

P

S

S

V

F

R

Q

I

V

V

V

G

A

V

V

E

E

P

P

R

F

L

N

G

S

-

P

-

V

-

I

-

S

-

G

-

N

-

P

-

G

-

P

H

H

K

K

V

I

Q

Q

G
|
G

L

I

K

G

N

-

M

-

Q

-

E

A

A

G

I

F

V

I

P

P

A

E

I

V

Y

L

N

R

P

T

A

D

A

L

L

I

D

D

L

E

K

V

L

I

T

T

V

V

Q

S

D

D

E

E

P

E

A

A

F

F

D

Q

T

F

A

G

R

R

D

R

L

L

A

A

V

K

R

E

E

E

G

G

L

L

F

L

V

S

G

G

M

I

S
|
S

S

S

G

G

A

A

A

N

M

L

A

C

G

A

A

A

I

I

R

Q

V

L

A

A

K

Q

D

R

-

P

A

E

P

N

A

E

G

G

S

K

T

L

I

I

V

V

L

I

L

Q

P
|
P

D

S

R

F

G

G

D

E

R

R

Y

Y

L

L

S

S

T

T

V

L

L

M

F

F

R

Q

S

N

V

I

active site: K46 (= K45), S269 (= S261)
binding pyridoxal-5'-phosphate: K46 (= K45), N77 (= N75), G181 (= G180), T182 (= T181), G183 (= G182), T185 (= T184), G225 (= G214), S269 (= S261), P296 (= P287)

4aecA Crystal structure of the arabidopsis thaliana o-acetyl-serine-(thiol)- lyasE C (see paper)
45% identity, 96% coverage: 7:302/308 of query aligns to 16:319/323 of 4aecA

query
sites
4aecA

S

N

L

V

S

S

G

Q

S

L

I

I

G

G

S

K

T

T

P

P

L

M

V

V

E

Y

I

L

R

N

K

S

L

I

N

A

P

K

N

G

P

C

K

V

V

A

R

N

I

I

L

A

A

A

K

K

L

L

E

E

G

I

N

M

N

E

V

P

G

C

S

S

V

V

K
|
K

D

D

R

R

P

I

A

G

L

Y

Y

S

M

M

L

V

A

T

K

D

A

A

E

E

A

Q

A

K

G

G

E

F

L

I

S

S

P

P

E

G

R

K

T

S

I

V

L

L

-

V

E

E

P

P

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

A

G

I

L

A

A

M

F

L

I

G

A

A

A

A

S

K

R

G

G

Y

Y

R

R

V

L

K

I

L

L

V

T

M

M

P

P

A

A

C

S

V

M

S

S

L

M

E

E

R

R

A

V

V

L

L

L

E

K

A

A

Y

F

G

G

A

A

E

E

L

L

V

V

L

L

S

T

P

D

A

P

E

A

E

K

A

G

T

M

D

T

G

G

A

A

I

V

R

Q

L

K

A

A

H

E

R

E

I

I

L

L

D

K

E

N

E

T

P

P

D

D

R

A

Y

Y

Y

-

M

M

P

L

N

Q

Q

Q

Y

F

A

D

N

N

P

P

N

A

N

N

P

P

L

K

A

I

H

H

Y

Y

E

E

T

T

G

G

P

P

E

E

I

I

Y

W

R

D

G

D

T

T

D

K

G

G

T

K

V

V

D

D

F

I

F

F

V

V

A

A

G

G

M
x
I

G
|
G

T
|
T

G
|
G

T
|
T

L

I

M

T

G

G

V

V

S

G

R

R

Y

F

F

I

R

K

E

E

T

K

K

N

P

P

S

K

V

T

R

Q

I

V

V

I

G

G

V

V

E

E

P

P

R

T

L

E

G

S

-

D

-

I

-

L

-

S

-

G

-

K

-

P

-

G

-

P

H

H

K

K

V

I

Q

Q

G
|
G

L

I

K

G

N

-

M

-

Q

-

E

A

A

G

I

F

V

I

P

P

A

K

I

N

Y

L

N

D

P

Q

A

K

A

I

L

M

D

D

L

E

K

V

L

I

T

A

V

I

Q

S

D

S

E

E

P

E

A

A

F

I

D

E

T

T

A

A

R

K

D

Q

L

L

A

A

V

L

R

K

E

E

G

G

L

L

F

M

V

V

G

G

M

I

S
|
S

S

S

G

G

A

A

M

A

A

A

G

A

A

A

I

I

R

K

V

V

A

A

K

K

D

R

-

P

A

E

P

N

A

A

G

G

S

K

T

L

I

I

V

A

V

V

L

V

L

F

P
|
P

D

S

R

F

G

G

D

E

R

R

Y

Y

L

L

S

S

T

T

V

P

L

L

F

F

R

Q

S

S

V

I

active site: K54 (= K45), S277 (= S261)
binding pyridoxal-5'-phosphate: K54 (= K45), N85 (= N75), I188 (≠ M179), G189 (= G180), T190 (= T181), G191 (= G182), G192 (= G183), T193 (= T184), G233 (= G214), S277 (= S261), P304 (= P287)

2efyA Crystal structure of t.Th. Hb8 o-acetylserine sulfhydrylase complexed with 4-acetylbutyric acid
45% identity, 95% coverage: 8:299/308 of query aligns to 3:301/302 of 2efyA

query
sites
2efyA

L

V

S

E

G

G

S

A

I

I

G

G

S

K

T

T

P

P

L

V

V

V

E

R

I

L

R

A

K

K

L

V

N

V

P

E

N

P

P

D

K

M

V

A

R

E

I

V

L

W

A

V

K

K

L

L

E

E

G

G

N

L

N

N

V

P

G

G

S

S

V

I

K
|
K

D

D

R

R

P

P

A

A

L

W

Y

Y

M

M

L

I

A

K

K

D

A

A

E

E

A

E

A

R

G

G

E

I

L

L

S

R

P

P

E

G

-

S

-

G

R

Q

T

V

I

I

L

V

E

E

P

P

T
|
T

S
|
S

G
|
G

N
|
N

T
|
T

G

G

I

I

A

G

I

L

A

A

M

M

L

I

G

A

A

A

A

S

K

R

G

G

Y

Y

R

R

V

L

K

I

L

L

V

T

M

M

P

P

A

A

C

Q

V

M

S

S

L

E

E

E

R

R

R

K

A

R

V

V

L

L

E

K

A

A

Y

F

G

G

A

A

E

E

L

L

V

V

L

L

S

T

P

D

A

P

E

E

E

R

A

R

T

M

D

L

G

A

A

A

I

R

R

E

L

E

A

A

H

L

R

R

I

L

L

K

D

E

E

E

E

L

P

G

D

A

R

-

Y

-

Y

F

M

M

P

P

N

D

Q
|
Q

Y

F

A

K

N

N

P

P

N

A

N

N

P

V

L

R

A

A

H

H

Y

Y

E

E

T

T

G

G

P

P

E

E

I

L

Y

Y

R

E

G

A

T

L

D

E

G

G

T

R

V

I

D

D

F

A

F

F

V

V

A
x
Y

G

G

M

S

G
|
G

T
|
T

G
|
G

G

G

T
|
T

L

I

M

T

G

G

V

V

S

G

R

R

Y

Y

F

L

R

K

E

E

T

R

K

I

P

P

S

H

V

V

R

K

I

V

V

I

G

A

V

V

E
|
E

P

P

-

A

-

R

-
x
S

-

N

-

V

-

L

-

S

-

G

R

K

L

M

G

G

H

Q

K

-

V

-

Q

H

G

G

L

F

K

Q

N
x
G

M
|
M

Q

G

E
x
P

A

G

I

F

V

I

P

P

A

E

I

N

Y

L

N

D

P

L

A

S

A

L

L

L

D

D

L

G

K

V

L

I

T

Q

V

V

Q

W

D

E

E

E

P

D

A

A

F

F

D

P

T

L

A

A

R

R

D

R

L

L

A

A

V

R

R

E

E

E

G

G

L

L

F

F

V

L

G

G

M

M

S
|
S

S

S

G

G

A

G

A

I

M

V

A

W

G

A

A

A

I

L

R

Q

V

V

A

A

K

R

D

E

A

L

P

G

A

P

G

G

S

K

T

R

I

V

V

A

V

C

L

I

L

S

P
|
P

D
|
D

R

G

G

G

D

W

R

K

Y

Y

L

L

S

S

T

T

V

P

L

L

F

Y

active site: K40 (= K45), S70 (= S73), E200 (= E205), S204 (vs. gap), S263 (= S261)
binding 5-oxohexanoic acid: T69 (= T72), G71 (= G74), T73 (= T76), Q141 (= Q146), G175 (= G180), G219 (≠ N217), M220 (= M218), P222 (≠ E220)
binding pyridoxal-5'-phosphate: K40 (= K45), N72 (= N75), Y172 (≠ A177), G175 (= G180), T176 (= T181), G177 (= G182), T179 (= T184), G219 (≠ N217), S263 (= S261), P289 (= P287), D290 (= D288)

2ecqA Crystal structure of t.Th. Hb8 o-acetylserine sulfhydrylase complexed with 3-hydroxylactate
45% identity, 95% coverage: 8:299/308 of query aligns to 3:301/302 of 2ecqA

query
sites
2ecqA

L

V

S

E

G

G

S

A

I

I

G

G

S

K

T

T

P

P

L

V

V

V

E

R

I

L

R

A

K

K

L

V

N

V

P

E

N

P

P

D

K

M

V

A

R

E

I

V

L

W

A

V

K

K

L

L

E

E

G

G

N

L

N

N

V

P

G

G

S

S

V

I

K
|
K

D

D

R

R

P

P

A

A

L

W

Y

Y

M

M

L

I

A

K

K

D

A

A

E

E

A

E

A

R

G

G

E

I

L

L

S

R

P

P

E

G

-

S

-

G

R

Q

T

V

I

I

L

V

E

E

P

P

T

T

S
|
S

G
|
G

N
|
N

T
|
T

G

G

I

I

A

G

I

L

A

A

M

M

L

I

G

A

A

A

A

S

K

R

G

G

Y

Y

R

R

V

L

K

I

L

L

V

T

M

M

P

P

A

A

C

Q

V

M

S

S

L

E

E

E

R

R

R

K

A

R

V

V

L

L

E

K

A

A

Y

F

G

G

A

A

E

E

L

L

V

V

L

L

S

T

P

D

A

P

E

E

E

R

A

R

T

M

D

L

G

A

A

A

I

R

R

E

L

E

A

A

H

L

R

R

I

L

L

K

D

E

E

E

E

L

P

G

D

A

R

-

Y

-

Y

F

M

M

P

P

N

D

Q
|
Q

Y

F

A

K

N

N

P

P

N

A

N

N

P

V

L

R

A

A

H

H

Y

Y

E

E

T

T

G

G

P

P

E

E

I

L

Y

Y

R

E

G

A

T

L

D

E

G

G

T

R

V

I

D

D

F

A

F

F

V

V

A
x
Y

G
|
G

M

S

G
|
G

T
|
T

G

G

T
|
T

L

I

M

T

G

G

V

V

S

G

R

R

Y

Y

F

L

R

K

E

E

T

R

K

I

P

P

S

H

V

V

R

K

I

V

V

I

G

A

V

V

E
|
E

P

P

-

A

-

R

-
x
S

-

N

-

V

-

L

-

S

-

G

R

K

L

M

G

G

H

Q

K

-

V

-

Q

H

G

G

L

F

K

Q

N
x
G

M

M

Q

G

E

P

A

G

I

F

V

I

P

P

A

E

I

N

Y

L

N

D

P

L

A

S

A

L

L

L

D

D

L

G

K

V

L

I

T

Q

V

V

Q

W

D

E

E

E

P

D

A

A

F

F

D

P

T

L

A

A

R

R

D

R

L

L

A

A

V

R

R

E

E

E

G

G

L

L

F

F

V

L

G

G

M

M

S
|
S

S

S

G

G

A

G

A

I

M

V

A

W

G

A

A

A

I

L

R

Q

V

V

A

A

K

R

D

E

A

L

P

G

A

P

G

G

S

K

T

R

I

V

V

A

V

C

L

I

L

S

P
|
P

D

D

R

G

G

G

D

W

R

K

Y

Y

L

L

S

S

T

T

V

P

L

L

F

Y

active site: K40 (= K45), S70 (= S73), E200 (= E205), S204 (vs. gap), S263 (= S261)
binding (3s)-3-hydroxybutanoic acid: K40 (= K45), G71 (= G74), T73 (= T76), Q141 (= Q146), G219 (≠ N217)
binding pyridoxal-5'-phosphate: K40 (= K45), N72 (= N75), Y172 (≠ A177), G173 (= G178), G175 (= G180), T176 (= T181), T179 (= T184), G219 (≠ N217), S263 (= S261), P289 (= P287)

2ecoA Crystal structure of t.Th. Hb8 o-acetylserine sulfhydrylase complexed with 4-methylvalerate
45% identity, 95% coverage: 8:299/308 of query aligns to 3:301/302 of 2ecoA

query
sites
2ecoA

L

V

S

E

G

G

S

A

I

I

G

G

S

K

T

T

P

P

L

V

V

V

E

R

I

L

R

A

K

K

L

V

N

V

P

E

N

P

P

D

K

M

V

A

R

E

I

V

L

W

A

V

K

K

L

L

E

E

G

G

N

L

N

N

V

P

G

G

S

S

V

I

K
|
K

D

D

R

R

P

P

A

A

L

W

Y

Y

M

M

L

I

A

K

K

D

A

A

E

E

A

E

A

R

G

G

E

I

L

L

S

R

P

P

E

G

-

S

-

G

R

Q

T

V

I

I

L

V

E

E

P

P

T
|
T

S
|
S

G
|
G

N
|
N

T
|
T

G

G

I

I

A

G

I

L

A

A

M

M

L

I

G

A

A

A

A

S

K

R

G

G

Y

Y

R

R

V

L

K

I

L

L

V

T

M

M

P

P

A

A

C

Q

V

M

S

S

L

E

E

E

R

R

R

K

A

R

V

V

L

L

E

K

A

A

Y

F

G

G

A

A

E

E

L

L

V

V

L

L

S

T

P

D

A

P

E

E

E

R

A

R

T

M

D

L

G

A

A

A

I

R

R

E

L

E

A

A

H

L

R

R

I

L

L

K

D

E

E

E

E

L

P

G

D

A

R

-

Y

-

Y

F

M

M

P

P

N

D

Q
|
Q

Y

F

A

K

N

N

P

P

N

A

N

N

P

V

L

R

A

A

H

H

Y

Y

E

E

T

T

G

G

P

P

E

E

I

L

Y

Y

R

E

G

A

T

L

D

E

G

G

T

R

V

I

D

D

F

A

F

F

V

V

A
x
Y

G

G

M

S

G
|
G

T
|
T

G

G

T
|
T

L

I

M

T

G

G

V

V

S

G

R

R

Y

Y

F

L

R

K

E

E

T

R

K

I

P

P

S

H

V

V

R

K

I

V

V

I

G

A

V

V

E
|
E

P

P

-

A

-

R

-
x
S

-

N

-

V

-

L

-

S

-

G

R

K

L

M

G

G

H

Q

K

-

V

-

Q

H

G

G

L

F

K

Q

N
x
G

M

M

Q

G

E

P

A

G

I

F

V

I

P

P

A

E

I

N

Y

L

N

D

P

L

A

S

A

L

L

L

D

D

L

G

K

V

L

I

T

Q

V

V

Q

W

D

E

E

E

P

D

A

A

F

F

D

P

T

L

A

A

R

R

D

R

L

L

A

A

V

R

R

E

E

E

G

G

L

L

F

F

V

L

G

G

M

M

S
|
S

S

S

G

G

A

G

A

I

M

V

A

W

G

A

A

A

I

L

R

Q

V

V

A

A

K

R

D

E

A

L

P

G

A

P

G

G

S

K

T

R

I

V

V

A

V

C

L

I

L

S

P
|
P

D
|
D

R

G

G

G

D

W

R

K

Y

Y

L

L

S

S

T

T

V

P

L

L

F

Y

active site: K40 (= K45), S70 (= S73), E200 (= E205), S204 (vs. gap), S263 (= S261)
binding 4-methyl valeric acid: K40 (= K45), T69 (= T72), G71 (= G74), T73 (= T76), Q141 (= Q146), G175 (= G180), T176 (= T181), G219 (≠ N217)
binding pyridoxal-5'-phosphate: K40 (= K45), N72 (= N75), Y172 (≠ A177), G175 (= G180), T176 (= T181), T179 (= T184), G219 (≠ N217), S263 (= S261), P289 (= P287), D290 (= D288)

P47999 Cysteine synthase, chloroplastic/chromoplastic; At.OAS.7-4; Beta-substituted Ala synthase 2;1; ARAth-Bsas2;1; CSase B; AtCS-B; CS-B; O-acetylserine (thiol)-lyase; O-acetylserine sulfhydrylase; OAS-TL B; cpACS1; EC 2.5.1.47 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
45% identity, 96% coverage: 12:306/308 of query aligns to 83:385/392 of P47999

query
sites
P47999

I

I

G

G

S

K

T

T

P

P

L

M

V

V

E

Y

I

L

R

N

K

N

L

V

N

V

P

K

N

G

P

C

K

V

V

A

R

S

I

V

L

A

A

A

K

K

L

L

E

E

G

I

N

M

N

E

V

P

G

C

S

S

V

V

K

K

D

D

R

R

P

I

A

G

L

Y

Y

S

M

M

L

I

A

T

K

D

A

A

E

E

A

E

A

K

G

G

E

L

L

I

S

T

P

P

E

G

R

K

T

S

I

V

L

L

-

V

E

E

P

S

T

T

S

S

G

G

N

N

T

T

G

G

I

I

A

G

I

L

A

A

M

F

L

I

G

A

A

A

A

S

K

K

G

G

Y

Y

R

K

V

L

K

I

L

L

V

T

M

M

P

P

A

A

C

S

V

M

S

S

L

L

E

E

R

R

A

V

V

L

L

L

E

R

A

A

Y

F

G

G

A

A

E

E

L

L

V

V

L

L

S

T

P

E

A

P

E

A

E

K

A

G

T

M

D

T

G

G

A

A

I

I

R

Q

L

K

A

A

H

E

R

E

I

I

L

L

D

K

E

K

E

T

P

P

D

N

R

S

Y

Y

Y

-

M

M

P

L

N

Q

Q

Q

Y

F

A

D

N

N

P

P

N

A

N

N

P

P

L

K

A

I

H

H

Y

Y

E

E

T

T

G

G

P

P

E

E

I

I

Y

W

R

E

G

D

T

T

D

R

G

G

T

K

V

I

D

D

F

I

F

L

V

V

A

A

G

G

M

I

G

G

T

T

G

G

T

T

L

I

M

T

G

G

V

V

S

G

R

R

Y

F

F

I

R

K

E

E

T

R

K

K

P

P

S

E

V

L

R

K

I

V

V

I

G

G

V

V

E

E

P

P

R

T

L

E

G

S

-

A

-

I

-

L

-

S

-

G

-

K

-

P

-

G

-

P

H

H

K

K

V

I

Q

Q

G

G

L

I

K

G

N

-

M

-

Q

-

E

A

A

G

I

F

V

V

P

P

A

K

I

N

Y

L

N

D

P

L

A

A

A

I

L

V

D

D

L

E

K

Y

L

I

T

A

V

I

Q

S

D

S

E

E

P

E

A

A

F

I

D

E

T

T

A

S

R

K

D

Q

L

L

A

A

V

L

R

Q

E

E

G

G

L

L

F

L

V

V

G

G

M

I

S

S

G

G

A

A

M

A

A

A

G

A

A

A

I

I

R

Q

V

V

A

A

K

K

D

R

-

P

A

E

P

N

A

A

G

G

S

K

T

L

I

I

V

A

V

V

L

V

L

F

P

P

D

S

R

F

G

G

D

E

R

R

Y

Y

L

L

S

S

T

T

V

Q

L

L

F

F

R

Q

S

S

V

I

C

R

G

E

K

E

C

C

Sites not aligning to the query:

61 modified: N-acetylalanine

4lmbA Crystal structure analysis of o-acetylserine sulfhydrylase cysk2 complexed with cystine from microcystis aeruginosa 7806 (see paper)
45% identity, 94% coverage: 12:300/308 of query aligns to 11:309/310 of 4lmbA

query
sites
4lmbA

I

V

G

G

S

R

T

T

P

P

L

L

V

V

E

Q

I

L

R

N

K

R

L

I

-

P

-

V

N

A

P

E

N

G

P

V

K

K

V

A

R

R

I

I

L

V

A

V

K

K

L

L

E

E

G

S

N

M

N

N

V

P

G

A

S

S

V

V

K
|
K

D

D

R

R

P

I

A

G

L

V

Y

S

M

M

L

V

A

E

K

D

A

A

E

E

A

A

G

G

E

L

L

I

S

H

P

P

E

D

R

K

T

T

I

I

L

L

-

V

E

E

P

P

T
|
T

S
|
S

G

G

N
|
N

T
|
T

G

G

I

I

A

A

I

L

A

A

M

M

L

V

G

A

A

A

K

K

G

G

Y

Y

R

R

V

L

K

V

L

L

V

T

M

M

P

P

A

E

C

T

V
x
M

S

S

L

L

E

E

R

R

A

A

V

M

L

L

E

K

A

A

Y

Y

G

G

A

A

E

Q

L

L

V

E

L

L

S

T

P

P

A

G

E

S

E

Q

A

G

T
x
M

D

E

G

G

A

A

I

I

R

T

L

R

A

A

H

E

R

E

I

I

L

V

D

E

E

N

E

T

P

P

D

N

R

A

Y

Y

Y

S

M

L

P

-

N

Q

Q
|
Q

Y
x
F

A

R

N

N

P

P

N

A

N

N

P

P

L

K

A

I

H

H

Y

R

E

E

T

T

G

A

P

E

E

E

I

I

Y

W

R

A

G

D

T

T

D

D

G

G

T

L

V

V

D

D

F

I

F

V

V

I

A

G

G

G

M
x
V

G
|
G

T
|
T

G
|
G

G

G

T
|
T

L

I

M

T

G

G

V

I

S

A

R

E

Y

T

F

I

R

K

E

P

T

R

K

R

P

P

S

Q

V

F

R

Q

I

A

V

I

G

A

V

V

E

E

P

P

R

S

L

N

G

S

-

P

-

V

-

L

-

S

-

G

-

Q

-

P

-

G

-

P

H

H

K

K

V

I

Q
|
Q

G
|
G

L
x
I

K

G

N

-

M

-

Q

-

E
x
A

A

G

I

F

V

I

P

P

A

A

I

I

Y

F

N

R

P

P

A

E

A

L

L

I

D

D

L

E

K

V

L

I

T

I

V

V

Q

D

D

D

E

T

P

E

A

A

F

F

D

A

T

Y

A

A

R

R

D

R

L

L

A

A

V

R

R

Q

E

E

G

G

L

L

F

L

V

S

G

G

M

I

S
|
S

S

A

G

G

A

A

M

L

A

W

G

A

A

A

I

I

R

Q

V

V

A

G

K

K

D

R

A

P

P

E

A

N

G

E

S

D

T

K

I

L

V

I

V

V

L

M

L

I

-

Q

P
|
P

D

S

R

F

G

G

D

E

R

R

Y

Y

L

L

S

S

T

T

V

A

L

L

F

F

R

K

active site: K46 (= K45), S269 (= S261)
binding cysteine: K46 (= K45), T74 (= T72), S75 (= S73), N77 (= N75), T78 (= T76), M101 (≠ V99), M125 (≠ T123), M125 (≠ T123), Q147 (= Q146), F148 (≠ Y147), Q224 (= Q213), G225 (= G214), G225 (= G214), I226 (≠ L215), A228 (≠ E220)
binding pyridoxal-5'-phosphate: K46 (= K45), N77 (= N75), V180 (≠ M179), G181 (= G180), T182 (= T181), G183 (= G182), T185 (= T184), G225 (= G214), S269 (= S261), P296 (= P287)

7n2tA O-acetylserine sulfhydrylase from citrullus vulgaris in the internal aldimine state, with citrate bound (see paper)
46% identity, 94% coverage: 12:302/308 of query aligns to 11:309/309 of 7n2tA

query
sites
7n2tA

I

I

G

G

S

N

T

T

P

P

L

L

V

V

E

Y

I

L

R

N

K

R

L

V

N

V

P

D

N

G

P

C

K

V

V

A

R

R

I

V

L

A

A

A

K

K

L

L

E

E

G

M

N

M

N

E

V

P

G

C

G

S

S

S

V

V

K

K

D

D

R

R

P

I

A

G

L

Y

Y

S

M

M

L

I

A

S

K

D

A

A

E

E

A

N

A

K

G

G

E

L

L

I

S

T

P

P

-

G

E

E

R

S

T

V

I

L

L

I

E

E

P

P

T

T

S

S

G

G

N

N

T

T

G

G

I

I

A

G

I

L

A

A

M

F

L

I

G

A

A

A

K

K

G

G

Y

Y

R

R

V

L

K

I

L

I

V

C

M

M

P

P

A

A

C

S

V

M

S

S

L

L

E

E

R

R

A

T

V

I

L

L

E

R

A

A

Y

F

G

G

A

A

E

E

L

L

V

V

L

L

S

T

P

D

A

P

E

A

E

R

A

G

T

M

D

K

G

G

A

A

I

V

R

Q

L

K

A

A

H

E

R

E

I

I

L

K

D

A

E

K

E

T

P

P

D

N

R

S

Y

Y

Y

I

M

L

P

-

N

Q

Q

Q

Y

F

A

E

N

N

P

P

N

A

N

N

P

P

L

K

A

I

H

H

Y

Y

E

E

T

T

G

G

P

P

E

E

I

I

Y

W

R

R

G

G

T

S

D

G

G

G

T

K

V

I

D

D

F

A

F

L

V

V

A

S

G

G

M

I

G

G

T

T

G

G

T

T

L

V

M

T

G

G

V

A

S

G

R

K

Y

Y

F

L

R

K

E

E

T

Q

K

N

P

P

S

N

V

I

R

K

I

L

V

Y

G

G

V

V

E

E

P

P

R

V

L

E

G

S

-

A

-

I

-

L

-

S

-

G

-

K

-

P

-

G

-

P

H

H

K

K

V

I

Q

Q

G

G

L

I

K

G

N

-

M

-

Q

-

E

A

A

G

I

F

V

I

P

P

A

G

I

V

Y

L

N

D

P

V

A

N

A

L

L

L

D

D

L

E

K

V

L

I

T

Q

V

V

Q

S

D

S

E

E

P

E

A

S

F

I

D

E

T

T

A

A

R

K

D

L

L

L

A

A

V

L

R

K

E

E

G

G

L

L

F

L

V

V

G

G

M

I

S

S

G

G

A

A

M

A

A

A

G

A

A

A

I

I

R
|
R

V

I

A

A

K

K

D
x
R

-

P

A

E

P

N

A

A

G

G

S

K

T

L

I

I

V

V

V

A

L

V

L

F

P

P

D

S

R

F

G

G

D

E

R

R

Y

Y

L

L

S

S

T

T

V

V

L

L

F

F

R

E

S

S

V

V

binding phosphate ion: R277 (= R271), R281 (≠ D275)

2isqA Crystal structure of o-acetylserine sulfhydrylase from arabidopsis thaliana in complex with c-terminal peptide from arabidopsis serine acetyltransferase (see paper)
44% identity, 94% coverage: 12:299/308 of query aligns to 11:306/320 of 2isqA

query
sites
2isqA

I

I

G

G

S

N

T

T

P

P

L

L

V

V

E

Y

I

L

R

N

K

N

L

V

N

A

P

E

N

G

P

C

K

V

V

G

R

R

I

V

L

A

A

A

K

K

L

L

E

E

G

M

N

M

N

E

V

P

G

C

G

S

S

S

V

V

K
|
K

D

D

R

R

P

I

A

G

L

F

Y

S

M

M

L

I

A

S

K

D

A

A

E

E

A

K

G

G

E

L

L

I

S

K

P

P

-

G

E

E

R

S

T

V

I

L

L

I

E

E

P

P

T
|
T

S
|
S

G
|
G

N
|
N

T
|
T

G

G

I

V

A

G

I

L

A

A

M

F

L

T

G

A

A

A

K

K

G

G

Y

Y

R

K

V

L

K

I

L

I

V

T

M

M

P

P

A

A

C

S

V

M

S

S

L

T

E

E

R

R

A

I

V

I

L

L

E

L

A

A

Y

F

G

G

A

V

E

E

L

L

V

V

L

L

S

T

P

D

A

P

E

A

E

K

A
x
G

T
x
M

D
x
K

G

G

A

A

I

I

R

A

L

K

A

A

H

E

R

E

I

I

L

L

D

A

E

K

E

T

P

P

D

N

R

G

Y

Y

Y

-

M

M

P

L

N

Q

Q

Q

Y

F

A

E

N

N

P

P

N

A

N

N

P

P

L

K

A

I

H

H

Y

Y

E

E

T

T

G

G

P

P

E

E

I

I

Y

W

R

K

G

G

T

T

D

G

G

G

T

K

V

I

D

D

F

G

F

F

V

V

A

S

G
|
G

M

I

G
|
G

T
|
T

G
|
G

G

G

T
|
T

L

I

M

T

G

G

V

A

S

G

R

K

Y

Y

F

L

R

K

E

E

T

Q

K

N

P

A

S

N

V

V

R

K

I

L

V

Y

G

G

V

V

E

E

P

P

R

V

L

E

G

S

-

A

-

I

-

L

-

S

-

G

-

K

-

P

-
x
G

-
x
P

H
|
H

K

K

V

I

Q
|
Q

G
|
G

L

I

K

-

N

-

M

-

Q

G

E

A

A

G

I

F

V

I

P

P

A

S

I

V

Y

L

N

N

P

V

A

D

A

L

L

I

D

D

L

E

K

V

L

V

T

Q

V

V

Q

S

D

S

E

D

P

E

A

S

F

I

D

D

T

M

A

A

R

R

D

Q

L

L

A

A

V

L

R

K

E

E

G

G

L

L

F

L

V

V

G

G

M

I

S
|
S

S

S

G

G

A

A

M

A

A

A

G

A

A

A

I

I

R

K

V

L

A

A

K

Q

D

R

-

P

A

E

P

N

A

A

G

G

S

K

T

L

I

F

V

V

V

A

L

I

L

F

P
|
P

D

S

R

F

G

G

D

E

R

R

Y

Y

L

L

S

S

T

T

V

V

L

L

F

F

active site: K44 (= K45), S267 (= S261)
binding pyridoxal-5'-phosphate: K44 (= K45), N75 (= N75), G177 (= G178), G179 (= G180), T180 (= T181), G181 (= G182), T183 (= T184), G223 (= G214), S267 (= S261), P294 (= P287)
binding : T72 (= T72), S73 (= S73), G74 (= G74), T76 (= T76), G122 (≠ A122), M123 (≠ T123), K124 (≠ D124), G217 (vs. gap), P218 (vs. gap), H219 (= H210), Q222 (= Q213), G223 (= G214)

P47998 Cysteine synthase 1; At.OAS.5-8; Beta-substituted Ala synthase 1;1; ARAth-Bsas1;1; CSase A; AtCS-A; Cys-3A; O-acetylserine (thiol)-lyase 1; OAS-TL A; O-acetylserine sulfhydrylase; Protein ONSET OF LEAF DEATH 3; EC 2.5.1.47 from Arabidopsis thaliana (Mouse-ear cress) (see 3 papers)
44% identity, 94% coverage: 12:299/308 of query aligns to 13:308/322 of P47998

query
sites
P47998

I

I

G

G

S

N

T

T

P

P

L

L

V

V

E

Y

I

L

R

N

K

N

L

V

N

A

P

E

N

G

P

C

K

V

V

G

R

R

I

V

L

A

A

A

K

K

L

L

E

E

G

M

N

M

N

E

V

P

G

C

G

S

S

S

V

V

K
|
K

D

D

R

R

P

I

A

G

L

F

Y

S

M

M

L

I

A

S

K

D

A

A

E

E

A

K

G

G

E

L

L

I

S

K

P

P

-

G

E

E

R

S

T

V

I

L

L

I

E

E

P

P

T
|
T

S
|
S

G

G

N
|
N

T
|
T

G

G

I

V

A

G

I

L

A

A

M

F

L

T

G

A

A

A

K

K

G

G

Y

Y

R

K

V

L

K

I

L

I

V

T

M

M

P

P

A

A

C

S

V

M

S

S

L

T

E

E

R

R

A

I

V

I

L

L

E

L

A

A

Y

F

G

G

A

V

E

E

L

L

V

V

L

L

S

T

P

D

A

P

E

A

E

K

A

G

T

M

D

K

G

G

A

A

I

I

R

A

L

K

A

A

H

E

R

E

I

I

L

L

D

A

E

K

E

T

P

P

D

N

R

G

Y

Y

Y

-

M

M

P

L

N

Q

Q
|
Q

Y

F

A

E

N

N

P

P

N

A

N

N

P

P

L

K

A

I

H
|
H

Y

Y

E

E

T

T

G
|
G

P

P

E

E

I

I

Y

W

R

K

G

G

T

T

D

G

G

G

T

K

V

I

D

D

F

G

F

F

V

V

A

S

G

G

M

I

G
|
G

T
|
T

G
|
G

T
|
T

L

I

M

T

G

G

V

A

S

G

R

K

Y

Y

F

L

R

K

E

E

T

Q

K

N

P

A

S

N

V

V

R

K

I

L

V

Y

G

G

V

V

E

E

P

P

R

V

L

E

G

S

-

A

-

I

-

L

-

S

-

G

-
x
K

-

P

-

G

-

P

H
|
H

K
|
K

V

I

Q

Q

G

G

L

I

K

-

N

-

M

-

Q

G

E

A

A

G

I

F

V

I

P

P

A

S

I

V

Y

L

N

N

P

V

A

D

A

L

L

I

D

D

L

E

K

V

L

V

T

Q

V

V

Q

S

D

S

E

D

P

E

A

S

F

I

D

D

T

M

A

A

R

R

D

Q

L

L

A

A

V

L

R

K

E

E

G

G

L

L

F

L

V

V

G

G

M

I

S
|
S

S

S

G

G

A

A

M

A

A

A

G

A

A

A

I

I

R

K

V

L

A

A

K

Q

D

R

-

P

A

E

P

N

A

A

G

G

S

K

T

L

I

F

V

V

V

A

L

I

L

F

P

P

D

S

R

F

G

G

D

E

R

R

Y

Y

L

L

S

S

T

T

V

V

L

L

F

F

K46 (= K45) modified: N6-(pyridoxal phosphate)lysine; mutation to A: No cysteine synthase activity.
T74 (= T72) mutation to A: Strong reduction of cysteine synthase activity.; mutation to S: Reduction of cysteine synthase activity.
S75 (= S73) mutation S->A,N,T: Strong reduction of cysteine synthase activity.
N77 (= N75) binding pyridoxal 5'-phosphate; mutation to A: Reduction of cysteine synthase activity.; mutation to D: Strong reduction of cysteine synthase activity.
T78 (= T76) mutation T->A,S: Reduction of cysteine synthase activity.
Q147 (= Q146) mutation Q->A,E: Strong reduction of cysteine synthase activity.
H157 (= H156) mutation H->Q,N: Slight reduction of cysteine synthase activity.
G162 (= G161) mutation to E: In old3-1; displays a early leaf death phenotype. Abolishes cysteine synthase activity.
GTGGT 181:185 (= GTGGT 180:184) binding pyridoxal 5'-phosphate
T182 (= T181) mutation T->A,S: Slight reduction of cysteine synthase activity.
T185 (= T184) mutation T->A,S: Strong reduction of cysteine synthase activity.
K217 (vs. gap) mutation to A: Impaired interaction with SAT1.
H221 (= H210) mutation to A: Impaired interaction with SAT1.
K222 (= K211) mutation to A: Impaired interaction with SAT1.
S269 (= S261) binding pyridoxal 5'-phosphate; mutation to A: Strong reduction of cysteine synthase activity.; mutation to T: Reduction of cysteine synthase activity.

P0ABK5 Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine sulfhydrylase A; S-carboxymethylcysteine synthase; Sulfate starvation-induced protein 5; SSI5; EC 2.5.1.47; EC 4.5.1.5 from Escherichia coli (strain K12) (see 5 papers)
44% identity, 94% coverage: 9:299/308 of query aligns to 9:312/323 of P0ABK5

query
sites
P0ABK5

S

S

G

L

S

T

I

I

G

G

S

H

T

T

P

P

L

L

V

V

E

R

I

L

R

N

K

R

L

I

N

G

P

N

N

G

P

-

K

-

V

-

R

R

I

I

L

L

A

A

K

K

L

V

E

E

G

S

N

R

N

N

V

P

G

S

G

F

S

S

V

V

K
|
K

D

C

R

R

P

I

A

G

L

A

Y

N

M

M

L

I

A

W

K

D

A

A

E

E

A

K

A

R

G

G

E

V

L

L

S

K

P

P

E

G

R

V

T

E

I

L

L

V

E

E

P

P

T

T

S

S

G

G

N

N

T

T

G

G

I

I

A

A

I

L

A

A

M

Y

L

V

G

A

A

A

K

R

G

G

Y

Y

R

K

V

L

K

T

L

L

V

T

M

M

P

P

A

E

C

T

V

M

S

S

L

I

E

E

R

R

A

K

V

L

L

L

E

K

A

A

Y

L

G

G

A

A

E

N

L

L

V

V

L

L

S

T

P

E

A

G

E

A

E

K

A

G

T

M

D

K

G

G

A

A

I

I

R

Q

L

K

A

A

H

E

R

E

I

I

L

V

D

A

E

S

E

N

P

P

D

E

R

K

Y

Y

Y

L

M

L

P

L

N

Q

Q

Q

Y

F

A

S

N

N

P

P

N

A

N

N

P

P

L

E

A

I

H

H

Y

E

E

K

T

T

G

G

P

P

E

E

I

I

Y

W

R

E

G

D

T

T

D

D

G

G

T

Q

V

V

D

D

F

V

F

F

V

I

A

A

G

G

M

V

G

G

T

T

G

G

T

T

L

L

M

T

G

G

V

V

S

S

R

R

Y

Y

F

I

R

K

E

G

T

T

K

K

P

G

S

K

V

T

R

D

I

L

-

I

-

S

V

V

G

A

V

V

E

E

P

P

R

T

L

D

G

S

-

P

-

V

-

I

-

A

-

Q

-

A

-

L

-

A

-

G

-

E

-

I

-

K

-

P

-

G

-

P

H

H

K

K

V

I

Q

Q

G

G

L

-

K

-

N

-

M

-

Q

-

E

-

A

-

I

-

V

I

P

G

A

A

I

G

Y

F

N

I

P

P

A

A

A

N

L

L

D

D

L

L

K

K

L

L

T

V

-

D

-

K

-

V

-

I

-

G

V

I

Q

T

D

N

E

E

P

E

A

A

F

I

D

S

T

T

A

A

R

R

D

R

L

L

A

M

V

E

R

E

E

E

G

G

L

I

F

L

V

A

G

G

M

I

S

S

G

G

A

A

M

V

A

A

G

A

A

A

I

L

R

K

V

L

A

Q

K

E

D

D

A

E

P

S

-

F

A

T

G

N

S

K

T

N

I

I

V

V

L

I

L

L

P

P

D

S

R

S

G

G

D

E

R

R

Y

Y

L

L

S

S

T

T

V

A

L

L

F

F

K42 (= K45) modified: N6-(pyridoxal phosphate)lysine; mutation to A: Still stimulates tRNase activity of CdiA-CT in vitro and in vivo.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

1z7yA Crystal structure of the arabidopsis thaliana o-acetylserine sulfhydrylase k46a mutant (see paper)
44% identity, 94% coverage: 12:299/308 of query aligns to 11:306/320 of 1z7yA

query
sites
1z7yA

I

I

G

G

S

N

T

T

P

P

L

L

V

V

E

Y

I

L

R

N

K

N

L

V

N

A

P

E

N

G

P

C

K

V

V

G

R

R

I

V

L

A

A

A

K

K

L

L

E

E

G

M

N

M

N

E

V

P

G

C

G

S

S

S

V

V

K
x
A

D

D

R

R

P

I

A

G

L

F

Y

S

M

M

L

I

A

S

K

D

A

A

E

E

A

K

G

G

E

L

L

I

S

K

P

P

-

G

E

E

R

S

T

V

I

L

L

I

E

E

P

P

T

T

S

S

G
|
G

N
|
N

T
|
T

G

G

I

V

A

G

I

L

A

A

M

F

L

T

G

A

A

A

K

K

G

G

Y

Y

R

K

V

L

K

I

L

I

V

T

M

M

P

P

A

A

C

S

V

M

S

S

L

T

E

E

R

R

A

I

V

I

L

L

E

L

A

A

Y

F

G

G

A

V

E

E

L

L

V

V

L

L

S

T

P

D

A

P

E

A

E

K

A

G

T

M

D

K

G

G

A

A

I

I

R

A

L

K

A

A

H

E

R

E

I

I

L

L

D

A

E

K

E

T

P

P

D

N

R

G

Y

Y

Y

-

M

M

P

L

N

Q

Q
|
Q

Y

F

A

E

N

N

P

P

N

A

N

N

P

P

L

K

A

I

H

H

Y

Y

E

E

T

T

G

G

P

P

E

E

I

I

Y

W

R

K

G

G

T

T

D

G

G

G

T

K

V

I

D

D

F

G

F

F

V

V

A

S

G

G

M
x
I

G
|
G

T
|
T

G
|
G

G

G

T
|
T

L

I

M

T

G

G

V

A

S

G

R

K

Y

Y

F

L

R

K

E

E

T

Q

K

N

P

A

S

N

V

V

R

K

I

L

V

Y

G

G

V

V

E

E

P

P

R

V

L

E

G

S

-

A

-

I

-

L

-

S

-

G

-

K

-

P

-

G

-

P

H

H

K

K

V

I

Q

Q

G
|
G

L

I

K

G

N

-

M

-

Q

-

E

A

A

G

I

F

V

I

P

P

A

S

I

V

Y

L

N

N

P

V

A

D

A

L

L

I

D

D

L

E

K

V

L

V

T

Q

V

V

Q

S

D

S

E

D

P

E

A

S

F

I

D

D

T

M

A

A

R

R

D

Q

L

L

A

A

V

L

R

K

E

E

G

G

L

L

F

L

V

V

G

G

M

I

S
|
S

S

S

G

G

A

A

M

A

A

A

G

A

A

A

I

I

R

K

V

L

A

A

K

Q

D

R

-

P

A

E

P

N

A

A

G

G

S

K

T

L

I

F

V

V

V

A

L

I

L

F

P
|
P

D
x
S

R

F

G

G

D

E

R

R

Y

Y

L

L

S

S

T

T

V

V

L

L

F

F

active site: A44 (≠ K45), S267 (= S261)
binding n-[(3-hydroxy-2-methyl-5-{[(trihydroxyphosphoranyl)oxy]methyl}pyridin-4-yl)methylene]methionine: G74 (= G74), N75 (= N75), T76 (= T76), Q145 (= Q146), I178 (≠ M179), G179 (= G180), T180 (= T181), G181 (= G182), T183 (= T184), G223 (= G214), S267 (= S261), P294 (= P287), S295 (≠ D288)

P0A1E3 Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine sulfhydrylase A; EC 2.5.1.47 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 4 papers)
43% identity, 94% coverage: 9:299/308 of query aligns to 9:312/323 of P0A1E3

query
sites
P0A1E3

S

S

G

L

S

T

I

I

G

G

S

H

T

T

P

P

L

L

V

V

E

R

I

L

R

N

K

R

L

I

N

G

P

N

N

G

P

-

K

-

V

-

R

R

I

I

L

L

A

A

K

K

L

V

E

E

G

S

N

R

N

N

V

P

G

S

G

F

S

S

V

V

K

K

D

C

R

R

P

I

A

G

L

A

Y

N

M

M

L

I

A

W

K

D

A

A

E

E

A

K

A

R

G

G

E

V

L

L

S

K

P

P

E

G

R

V

T

E

I

L

L

V

E

E

P

P

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

A

A

I

L

A

A

M

Y

L

V

G

A

A

A

K

R

G

G

Y

Y

R

K

V

L

K

T

L

L

V

T

M

M

P

P

A

E

C

T

V

M

S

S

L

I

E

E

R

R

A

K

V

L

L

L

E

K

A

A

Y

L

G

G

A

A

E

N

L

L

V

V

L

L

S

T

P

E

A

G

E

A

E

K

A

G

T

M

D

K

G

G

A

A

I

I

R

Q

L

K

A

A

H

E

R

E

I

I

L

V

D

A

E

S

E

D

P

P

D

Q

R

K

Y

Y

Y

L

M

L

P

L

N

Q

Q

Q

Y

F

A

S

N

N

P

P

N

A

N

N

P

P

L

E

A

I

H

H

Y

E

E

K

T

T

G

G

P

P

E

E

I

I

Y

W

R

E

G

D

T

T

D

D

G

G

T

Q

V

V

D

D

F

V

F

F

V

I

A

S

G

G

M

V

G

G

T

T

G

G

T

T

L

L

M

T

G

G

V

V

S

T

R

R

Y

Y

F

I

R

K

E

G

T

T

K

K

P

G

S

K

V

T

R

D

I

L

-

I

-

T

V

V

G

A

V

V

E

E

P

P

R

T

L

D

G

S

-

P

-

V

-

I

-

A

-

Q

-

A

-

L

-

A

-

G

-

E

-

I

-

K

-

P

-

G

-

P

H

H

K

K

V

I

Q

Q

G

G

L

-

K

-

N

-

M

-

Q

-

E

-

A

-

I

-

V

I

P

G

A

A

I

G

Y

F

N

I

P

P

A

G

A

N

L

L

D

D

L

L

K

K

L

L

T

I

-

D

-

K

-

V

-

G

V

I

Q

T

D

N

E

E

P

E

A

A

F

I

D

S

T

T

A

A

R

R

D

R

L

L

A

M

V

E

R

E

E

E

G

G

L

I

F

L

V

A

G

G

M

I

S
|
S

S

S

G

G

A

A

M

V

A

A

G

A

A

A

I

L

R

K

V

L

A

Q

K

E

D

D

A

E

P

S

-

F

A

T

G

N

S

K

T

N

I

I

V

V

L

I

L

L

P

P

D

S

R

S

G

G

D

E

R

R

Y

Y

L

L

S

S

T

T

V

A

L

L

F

F

N72 (= N75) binding pyridoxal 5'-phosphate
S273 (= S261) binding pyridoxal 5'-phosphate

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

Query Sequence

>WP_004513581.1 NCBI__GCF_000012925.1:WP_004513581.1
MEHTPFSLSGSIGSTPLVEIRKLNPNPKVRILAKLEGNNVGGSVKDRPALYMLAKAEAAG
ELSPERTILEPTSGNTGIAIAMLGAAKGYRVKLVMPACVSLERRAVLEAYGAELVLSPAE
EATDGAIRLAHRILDEEPDRYYMPNQYANPNNPLAHYETTGPEIYRGTDGTVDFFVAGMG
TGGTLMGVSRYFRETKPSVRIVGVEPRLGHKVQGLKNMQEAIVPAIYNPAALDLKLTVQD
EPAFDTARDLAVREGLFVGMSSGAAMAGAIRVAKDAPAGSTIVVLLPDRGDRYLSTVLFR
SVCGKCPP

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory