SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_004514479.1 NCBI__GCF_000012925.1:WP_004514479.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

5xoqA Crystal structure of o-acetylserine sulfhydrylase with bound transcription factor peptide inhibitor from planctomyces limnophilus
59% identity, 100% coverage: 1:307/307 of query aligns to 2:307/310 of 5xoqA

query
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5xoqA

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binding : T72 (= T73), S73 (= S74), G74 (= G75), T76 (= T77), M123 (= M124), Q144 (= Q145), R218 (≠ P219), H219 (= H220), Q222 (= Q223), G223 (= G224), A226 (= A227)

2q3dA 2.2 a resolution crystal structure of o-acetylserine sulfhydrylase (oass) from mycobacterium tuberculosis in complex with the reaction intermediate alpha-aminoacrylate (see paper)
59% identity, 98% coverage: 6:306/307 of query aligns to 4:305/306 of 2q3dA

query
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2q3dA

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active site: K44 (= K46), S266 (= S268), P293 (≠ C294)
binding 2-[(3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl)-amino]-propionic acid: K44 (= K46), T71 (= T73), S72 (= S74), N74 (= N76), T75 (= T77), Q144 (= Q145), V177 (= V179), G178 (= G180), T179 (= T181), G180 (= G182), T182 (= T184), G222 (= G224), I223 (= I225), S266 (= S268), P293 (≠ C294), D294 (= D295)

P9WP55 O-acetylserine sulfhydrylase; OAS sulfhydrylase; OASS; Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine-specific cysteine synthase; Sulfide-dependent cysteine synthase; EC 2.5.1.47 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
59% identity, 98% coverage: 6:306/307 of query aligns to 4:305/310 of P9WP55

query
sites
P9WP55

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K44 (= K46) modified: N6-(pyridoxal phosphate)lysine
N74 (= N76) binding pyridoxal 5'-phosphate
GTGGT 178:182 (= GTGGT 180:184) binding pyridoxal 5'-phosphate
S266 (= S268) binding pyridoxal 5'-phosphate

3zeiA Structure of the mycobacterium tuberculosis o-acetylserine sulfhydrylase (oass) cysk1 in complex with a small molecule inhibitor (see paper)
59% identity, 96% coverage: 6:301/307 of query aligns to 4:300/300 of 3zeiA

query
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3zeiA

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active site: K44 (= K46), S266 (= S268), P293 (≠ C294)
binding 3-[(Z)-[(5Z)-5-[[2-(2-hydroxy-2-oxoethyloxy)phenyl]methylidene]-3-methyl-4-oxidanylidene-1,3-thiazolidin-2-ylidene]amino]benzoic acid: T71 (= T73), S72 (= S74), I126 (≠ V128), Q144 (= Q145), F145 (= F146), K215 (≠ P217), G222 (= G224), A225 (= A227), F227 (= F229)
binding pyridoxal-5'-phosphate: K44 (= K46), N74 (= N76), V177 (= V179), G178 (= G180), T179 (= T181), G180 (= G182), T182 (= T184), G222 (= G224), S266 (= S268), P293 (≠ C294), D294 (= D295)

2q3cA 2.1 a resolution crystal structure of o-acetylserine sulfhydrylase (oass) holoenzyme from mycobacterium tuberculosis in complex with the inhibitory peptide dfsi (see paper)
59% identity, 96% coverage: 6:301/307 of query aligns to 4:300/300 of 2q3cA

query
sites
2q3cA

S

A

D

E

K

D

V

I

I

T

E

Q

Q

L

I

I

G

G

N

R

T

T

P

P

L

L

Q

V

R

R

L

L

A

R

K

R

L

V

E

T

A

D

P

G

G

A

S

V

A

A

A

D

V

I

Y

V

G

A

K

K

A

L

E

E

F

F

N

N

P

P

G

A

G

N

S

S

V

V

K
|
K

D

D

R

R

I

I

C

G

L

V

A

A

M

M

I

L

E

Q

A

A

E

E

K

Q

D

A

G

G

S

L

L

I

K

K

P

P

G

D

M

T

T

I

V

I

V

L

E

E

P

P

T
|
T

S
|
S

G
|
G

N

N

T
|
T

G

G

I

I

G

A

L

L

S

A

M

M

I

V

C

C

A

A

I

A

K

R

G

G

Y

Y

K

R

L

C

V

V

L

L

T

T

M

M

P

P

D

E

T

T

M

M

T

S

V

L

E

E

R

R

R

M

L

L

A

R

A

A

Y

Y

G

G

A

A

E

E

L

L

V

I

L

L

T

T

P

P

G

G

A

A

Q

D

G

G

M
|
M

R

S

G

G

A

A

V

I

Q

A

K

K

A

A

E

E

L

L

A

A

-

K

A

T

Q

D

P

Q

G

R

H

Y

I

F

M

V

M

P

Q

Q

Q
|
Q

F

F

K

E

N

N

G

P

A

A

N

N

P

P

E

A

A

I

H

H

R

R

R

V

T

T

G

A

P

E

E

E

I

V

V

W

R

R

A

D

L

T

S

D

G

G

Q

K

S

-

I

V

D

D

A

I

F

V

V

V

A

A

G

G

V

V

G

G

T

T

G

G

T

T

I

I

T

T

G

G

V

V

G

A

E

Q

V

V

L

I

K

K

E

E

H

R

N

K

A

P

A

S

V

A

R

R

I

F

V

V

A

A

V

V

E

E

P

P

D

A

A

A

S

S

P

P

I

V

L

L

S

S

G

G

Q

Q

P
x
K

G

G

P

P

H

H

K

P

I

I

Q

Q

G
|
G

I

I

G

G

A
|
A

G

G

F

F

V

V

P

P

D

P

V

V

L

L

N

D

T

Q

K

D

V

L

Y

V

D

D

E

E

V

I

I

I

R

T

V

V

T

G

N

N

E

E

D

D

A

A

Y

L

E

N

T

V

V

A

R

R

L

L

A

A

Q

R

E

E

G

G

V

L

L

L

V

V

G

G

I

I

S
|
S

S

S

G

G

A

A

N

A

V

T

F

V

A

A

L

L

Q

Q

V

V

A

A

K

R

R

R

-

P

L

E

G

N

P

A

G

G

K

K

N

L

V

I

V

V

T

V

I

V

L

L

C
x
P

D

D

T

F

G

G

E

E

R

R

Y

Y

L

L

active site: K44 (= K46), S266 (= S268), P293 (≠ C294)
binding : T71 (= T73), S72 (= S74), G73 (= G75), T75 (= T77), M122 (= M124), Q144 (= Q145), K215 (≠ P217), G222 (= G224), A225 (= A227)

4lmaA Crystal structure analysis of o-acetylserine sulfhydrylase cysk1 from microcystis aeruginosa 7806 (see paper)
56% identity, 98% coverage: 6:307/307 of query aligns to 4:309/318 of 4lmaA

query
sites
4lmaA

S

A

D

H

K

D

V

V

I

T

E

E

Q

L

I

V

G

G

N

R

T

T

P

P

L

L

Q

V

R

Q

L

L

A

N

K

R

L

I

-

P

E

Q

A

A

P

E

G

G

S

C

A

L

A

G

-

R

V

I

Y

V

G

M

K

K

A

L

E

E

F

G

F

M

N

N

P

P

G

A

S

S

V

V

K
|
K

D

D

R

R

I

I

C

G

L

T

A

H

M

M

I

I

E

N

A

S

A

A

E

E

K

K

D

A

G

G

S

L

L

I

K

N

P

P

G

E

M

T

T

T

V

V

-

L

V

V

E

E

P

P

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

G

A

L

L

S

A

M

M

I

T

C

A

A

A

I

A

K

K

G

G

Y

Y

K

R

L

L

V

I

L

L

T

T

M

M

P

P

D

E

T

T

M

M

T

S

V

L

E

E

R

R

R

A

L

M

L

L

A

K

A

A

Y

Y

G

G

A

A

E

T

L

L

V

E

L

L

T

T

P

P

G

G

A

S

Q

Q

G

G

M

M

R

K

G

G

A

A

V

I

Q

L

K

R

A

A

E

Q

L

I

A

V

-

D

A

S

Q

I

P

P

G

G

H

A

I

Y

M

M

M

L

Q

Q

F

F

K

R

N

N

G

P

A

S

N

N

P

P

E

E

A

I

H

H

R

R

R

L

T

T

G

A

P

E

E

E

I

I

V

W

R

Q

A

D

L

T

S

E

G

G

Q

Q

S

-

I

V

D

D

A

F

F

I

V

V

A

A

G

G

V

V

G
|
G

T
|
T

G
|
G

G

G

T
|
T

I

I

T

T

G

G

V

V

G

A

E

E

V

V

L

I

K

K

E

S

H

R

N

K

A

P

A

S

V

F

R

Q

I

V

V

V

A

A

V

V

E

E

P

P

D

F

A

N

S

S

P

P

I

V

L

I

S

S

G

G

Q

N

P

P

G

G

P

P

H

H

K

K

I

I

Q

Q

G
|
G

I

I

G

G

A

A

G

G

F

F

V

I

P

P

D

E

V

V

L

L

N

R

T

T

K

D

V

L

Y

I

D

D

E

E

V

V

I

I

R

T

V

V

T

S

N

D

E

E

D

E

A

A

Y

F

E

Q

T

F

V

G

R

R

L

L

A

A

Q

K

E

E

G

G

V

L

L

L

V

S

G

G

I

I

S
|
S

S

S

G

G

A

A

N

N

V

L

F

C

A

A

L

I

Q

Q

V

L

A

A

K

Q

R

R

-

P

L

E

G

N

P

E

G

G

K

K

N

L

V

I

V

V

T

V

I

I

L

Q

C
x
P

D

S

T

F

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

G

L

V

M

F

F

D

Q

active site: K46 (= K46), S269 (= S268)
binding pyridoxal-5'-phosphate: K46 (= K46), N77 (= N76), G181 (= G180), T182 (= T181), G183 (= G182), T185 (= T184), G225 (= G224), S269 (= S268), P296 (≠ C294)

P47998 Cysteine synthase 1; At.OAS.5-8; Beta-substituted Ala synthase 1;1; ARAth-Bsas1;1; CSase A; AtCS-A; Cys-3A; O-acetylserine (thiol)-lyase 1; OAS-TL A; O-acetylserine sulfhydrylase; Protein ONSET OF LEAF DEATH 3; EC 2.5.1.47 from Arabidopsis thaliana (Mouse-ear cress) (see 3 papers)
56% identity, 97% coverage: 9:307/307 of query aligns to 9:309/322 of P47998

query
sites
P47998

V

V

I

T

E

E

Q

L

I

I

G

G

N

N

T

T

P

P

L

L

Q

V

R

Y

L

L

A

N

K

N

L

V

E

A

A

E

P

G

G

C

S

V

A

G

A

R

V

V

Y

A

G

A

K

K

A

L

E

E

F

M

N

E

P

P

G

C

G

S

S

S

V

V

K
|
K

D

D

R

R

I

I

C

G

L

F

A

S

M

M

I

I

E

S

A

D

A

A

E

E

K

K

D

K

G

G

S

L

L

I

K

K

P

P

G

G

M

E

T

S

V

V

-

L

V

I

E

E

P

P

T
|
T

S
|
S

G

G

N
|
N

T
|
T

G

G

I

V

G

G

L

L

S

A

M

F

I

T

C

A

A

A

I

A

K

K

G

G

Y

Y

K

K

L

L

V

I

L

I

T

T

M

M

P

P

D

A

T

S

M

M

T

S

V

T

E

E

R

R

R

I

L

I

L

L

A

L

A

A

Y

F

G

G

A

V

E

E

L

L

V

V

L

L

T

T

P

D

G

P

A

A

Q

K

G

G

M

M

R

K

G

G

A

A

V

I

Q

A

K

K

A

A

E

E

-

I

L

L

A

A

A

K

Q

T

P

P

G

N

H

G

I

Y

M

M

M

L

Q

Q

Q
|
Q

F

F

K

E

N

N

G

P

A

A

N

N

P

P

E

K

A

I

H
|
H

R

Y

R

E

T

T

G
|
G

P

P

E

E

I

I

V

W

R

K

A

G

L

T

S

G

G

G

Q

K

S

-

I

I

D

D

A

G

F

F

V

V

A

S

G

G

V

I

G
|
G

T
|
T

G
|
G

T
|
T

I

I

T

T

G

G

V

A

G

G

E

K

V

Y

L

L

K

K

E

E

H

Q

N

N

A

A

A

N

V

V

R

K

I

L

V

Y

A

G

V

V

E

E

P

P

D

V

A

E

S

S

P

A

I

I

L

L

S

S

G

G

Q
x
K

P

P

G

G

P

P

H
|
H

K
|
K

I

I

Q

Q

G

G

I

I

G

G

A

A

G

G

F

F

V

I

P

P

D

S

V

V

L

L

N

N

T

V

K

D

V

L

Y

I

D

D

E

E

V

V

I

V

R

Q

V

V

T

S

N

S

E

D

D

E

A

S

Y

I

E

D

T

M

V

A

R

R

R

Q

L

L

A

A

Q

L

E

K

E

E

G

G

V

L

L

L

V

V

G

G

I

I

S
|
S

S

S

G

G

A

A

N

A

V

A

F

A

A

A

L

I

Q

K

V

L

A

A

K

Q

R

R

-

P

L

E

G

N

P

A

G

G

K

K

N

L

V

F

V

V

T

A

I

I

L

F

C

P

D

S

T

F

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

G

V

V

L

F

F

D

D

K46 (= K46) modified: N6-(pyridoxal phosphate)lysine; mutation to A: No cysteine synthase activity.
T74 (= T73) mutation to A: Strong reduction of cysteine synthase activity.; mutation to S: Reduction of cysteine synthase activity.
S75 (= S74) mutation S->A,N,T: Strong reduction of cysteine synthase activity.
N77 (= N76) binding pyridoxal 5'-phosphate; mutation to A: Reduction of cysteine synthase activity.; mutation to D: Strong reduction of cysteine synthase activity.
T78 (= T77) mutation T->A,S: Reduction of cysteine synthase activity.
Q147 (= Q145) mutation Q->A,E: Strong reduction of cysteine synthase activity.
H157 (= H155) mutation H->Q,N: Slight reduction of cysteine synthase activity.
G162 (= G160) mutation to E: In old3-1; displays a early leaf death phenotype. Abolishes cysteine synthase activity.
GTGGT 181:185 (= GTGGT 180:184) binding pyridoxal 5'-phosphate
T182 (= T181) mutation T->A,S: Slight reduction of cysteine synthase activity.
T185 (= T184) mutation T->A,S: Strong reduction of cysteine synthase activity.
K217 (≠ Q216) mutation to A: Impaired interaction with SAT1.
H221 (= H220) mutation to A: Impaired interaction with SAT1.
K222 (= K221) mutation to A: Impaired interaction with SAT1.
S269 (= S268) binding pyridoxal 5'-phosphate; mutation to A: Strong reduction of cysteine synthase activity.; mutation to T: Reduction of cysteine synthase activity.

2isqA Crystal structure of o-acetylserine sulfhydrylase from arabidopsis thaliana in complex with c-terminal peptide from arabidopsis serine acetyltransferase (see paper)
56% identity, 97% coverage: 9:307/307 of query aligns to 7:307/320 of 2isqA

query
sites
2isqA

V

V

I

T

E

E

Q

L

I

I

G

G

N

N

T

T

P

P

L

L

Q

V

R

Y

L

L

A

N

K

N

L

V

E

A

A

E

P

G

G

C

S

V

A

G

A

R

V

V

Y

A

G

A

K

K

A

L

E

E

F

M

N

E

P

P

G

C

G

S

S

S

V

V

K
|
K

D

D

R

R

I

I

C

G

L

F

A

S

M

M

I

I

E

S

A

D

A

A

E

E

K

K

D

K

G

G

S

L

L

I

K

K

P

P

G

G

M

E

T

S

V

V

-

L

V

I

E

E

P

P

T
|
T

S
|
S

G
|
G

N
|
N

T
|
T

G

G

I

V

G

G

L

L

S

A

M

F

I

T

C

A

A

A

I

A

K

K

G

G

Y

Y

K

K

L

L

V

I

L

I

T

T

M

M

P

P

D

A

T

S

M

M

T

S

V

T

E

E

R

R

R

I

L

I

L

L

A

L

A

A

Y

F

G

G

A

V

E

E

L

L

V

V

L

L

T

T

P

D

G

P

A

A

Q

K

G
|
G

M
|
M

R
x
K

G

G

A

A

V

I

Q

A

K

K

A

A

E

E

-

I

L

L

A

A

A

K

Q

T

P

P

G

N

H

G

I

Y

M

M

M

L

Q

Q

F

F

K

E

N

N

G

P

A

A

N

N

P

P

E

K

A

I

H

H

R

Y

R

E

T

T

G

G

P

P

E

E

I

I

V

W

R

K

A

G

L

T

S

G

G

G

Q

K

S

-

I

I

D

D

A

G

F

F

V

V

A

S

G
|
G

V

I

G
|
G

T
|
T

G
|
G

G

G

T
|
T

I

I

T

T

G

G

V

A

G

G

E

K

V

Y

L

L

K

K

E

E

H

Q

N

N

A

A

A

N

V

V

R

K

I

L

V

Y

A

G

V

V

E

E

P

P

D

V

A

E

S

S

P

A

I

I

L

L

S

S

G

G

Q

K

P

P

G
|
G

P
|
P

H
|
H

K

K

I

I

Q
|
Q

G
|
G

I

I

G

G

A

A

G

G

F

F

V

I

P

P

D

S

V

V

L

L

N

N

T

V

K

D

V

L

Y

I

D

D

E

E

V

V

I

V

R

Q

V

V

T

S

N

S

E

D

D

E

A

S

Y

I

E

D

T

M

V

A

R

R

R

Q

L

L

A

A

Q

L

E

K

E

E

G

G

V

L

L

L

V

V

G

G

I

I

S
|
S

S

S

G

G

A

A

N

A

V

A

F

A

A

A

L

I

Q

K

V

L

A

A

K

Q

R

R

-

P

L

E

G

N

P

A

G

G

K

K

N

L

V

F

V

V

T

A

I

I

L

F

C
x
P

D

S

T

F

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

G

V

V

L

F

F

D

D

active site: K44 (= K46), S267 (= S268)
binding pyridoxal-5'-phosphate: K44 (= K46), N75 (= N76), G177 (= G178), G179 (= G180), T180 (= T181), G181 (= G182), T183 (= T184), G223 (= G224), S267 (= S268), P294 (≠ C294)
binding : T72 (= T73), S73 (= S74), G74 (= G75), T76 (= T77), G122 (= G123), M123 (= M124), K124 (≠ R125), G217 (= G218), P218 (= P219), H219 (= H220), Q222 (= Q223), G223 (= G224)

1z7yA Crystal structure of the arabidopsis thaliana o-acetylserine sulfhydrylase k46a mutant (see paper)
55% identity, 97% coverage: 9:307/307 of query aligns to 7:307/320 of 1z7yA

query
sites
1z7yA

V

V

I

T

E

E

Q

L

I

I

G

G

N

N

T

T

P

P

L

L

Q

V

R

Y

L

L

A

N

K

N

L

V

E

A

A

E

P

G

G

C

S

V

A

G

A

R

V

V

Y

A

G

A

K

K

A

L

E

E

F

M

N

E

P

P

G

C

G

S

S

S

V

V

K
x
A

D

D

R

R

I

I

C

G

L

F

A

S

M

M

I

I

E

S

A

D

A

A

E

E

K

K

D

K

G

G

S

L

L

I

K

K

P

P

G

G

M

E

T

S

V

V

-

L

V

I

E

E

P

P

T

T

S

S

G
|
G

N
|
N

T
|
T

G

G

I

V

G

G

L

L

S

A

M

F

I

T

C

A

A

A

I

A

K

K

G

G

Y

Y

K

K

L

L

V

I

L

I

T

T

M

M

P

P

D

A

T

S

M

M

T

S

V

T

E

E

R

R

R

I

L

I

L

L

A

L

A

A

Y

F

G

G

A

V

E

E

L

L

V

V

L

L

T

T

P

D

G

P

A

A

Q

K

G

G

M

M

R

K

G

G

A

A

V

I

Q

A

K

K

A

A

E

E

-

I

L

L

A

A

A

K

Q

T

P

P

G

N

H

G

I

Y

M

M

M

L

Q

Q

Q
|
Q

F

F

K

E

N

N

G

P

A

A

N

N

P

P

E

K

A

I

H

H

R

Y

R

E

T

T

G

G

P

P

E

E

I

I

V

W

R

K

A

G

L

T

S

G

G

G

Q

K

S

-

I

I

D

D

A

G

F

F

V

V

A

S

G

G

V
x
I

G
|
G

T
|
T

G
|
G

G

G

T
|
T

I

I

T

T

G

G

V

A

G

G

E

K

V

Y

L

L

K

K

E

E

H

Q

N

N

A

A

A

N

V

V

R

K

I

L

V

Y

A

G

V

V

E

E

P

P

D

V

A

E

S

S

P

A

I

I

L

L

S

S

G

G

Q

K

P

P

G

G

P

P

H

H

K

K

I

I

Q

Q

G
|
G

I

I

G

G

A

A

G

G

F

F

V

I

P

P

D

S

V

V

L

L

N

N

T

V

K

D

V

L

Y

I

D

D

E

E

V

V

I

V

R

Q

V

V

T

S

N

S

E

D

D

E

A

S

Y

I

E

D

T

M

V

A

R

R

R

Q

L

L

A

A

Q

L

E

K

E

E

G

G

V

L

L

L

V

V

G

G

I

I

S
|
S

S

S

G

G

A

A

N

A

V

A

F

A

A

A

L

I

Q

K

V

L

A

A

K

Q

R

R

-

P

L

E

G

N

P

A

G

G

K

K

N

L

V

F

V

V

T

A

I

I

L

F

C
x
P

D
x
S

T

F

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

G

V

V

L

F

F

D

D

active site: A44 (≠ K46), S267 (= S268)
binding n-[(3-hydroxy-2-methyl-5-{[(trihydroxyphosphoranyl)oxy]methyl}pyridin-4-yl)methylene]methionine: G74 (= G75), N75 (= N76), T76 (= T77), Q145 (= Q145), I178 (≠ V179), G179 (= G180), T180 (= T181), G181 (= G182), T183 (= T184), G223 (= G224), S267 (= S268), P294 (≠ C294), S295 (≠ D295)

Q93244 Cysteine synthase 1; O-acetylserine (thiol)-lyase 1; OAS-TL; EC 2.5.1.47 from Caenorhabditis elegans (see 2 papers)
56% identity, 96% coverage: 13:306/307 of query aligns to 15:310/341 of Q93244

query
sites
Q93244

I

I

G

G

N

N

T

T

P

P

L

M

Q

V

R

Y

L

I

A

N

K

K

L

L

E

T

A

K

-

G

-

L

P

P

G

G

S

T

A

V

A

A

V

V

Y

-

G

-

K

K

A

I

E

E

F

Y

F

M

N

N

P

P

G

A

G

G

S

S

V

V

K

K

D

D

R

R

I

I

C

G

L

A

A

A

M

M

I

L

E

A

A

A

E

E

K

K

D

D

G

G

S

T

L

V

K

I

P

P

G

G

M

V

T

T

-

T

V

L

V

I

E

E

P
|
P

T

T

S

S

G

G

N

N

T

T

G

G

I

I

G

A

L

L

S

A

M

F

I

V

C
x
A

A

A

I

A

K

K

G

G

Y

Y

K

R

L

C

V

I

L

V

T

T

M

M

P

P

D

A

T

S

M

M

T

S

V

G

E

E

R

R

R

T

L

L

A

K

A

A

Y

Y

G

G

A

S

E

E

L

V

V

V

L

L

T

T

P

D

G

P

A

A

Q

K

G

G

M

M

R

K

G

G

A

A

V

I

Q

D

K

M

A

A

E

N

E

Q

L

L

A

K

A

E

Q

N

-

I

P

P

G

G

H
x
S

I

I

M

I

M

L

Q

A

Q

Q

F

F

K

D

N

N

G

P

A

N

N

N

P

P

E

L

A

V

H

H

R

Y

R

Q

T

T

G

G

P

P

E

E

I

I

V

W

R

R

A

Q

L

T

S

K

G

G

Q

-

S

T

I

V

D

D

A

A

F

V

V

V

A

F

G
|
G

V

V

G
|
G

T

T

G

G

T

T

I

I

T

T

G

G

V

V

G

G

E

R

V

Y

L

L

K

Q

E

E

H

Q

N

N

A

P

A

G

V

V

R

R

I

V

V

F

A

A

V

V

E

E

P

P

D

E

A

E

S

S

P

A

I

I

L

L

S

S

G

G

G

R

Q

P

P

A

G

G

P

P

H

H

K

K

I

I

Q

Q

G

G

I

I

G
|
G

A

A

G

G

F

F

V

A

P

P

D

A

V

V

L

L

N

D

T

T

K

K

V

I

Y

Y

D

E

E

D

V

V

I

I

R

R

V

I

T

H

N

S

E

D

D

E

A

A

Y

I

E

V

T

M

V

A

R

Q

R
|
R

L

L

A

S

Q

Y

E

E

G

G

V

L

L

L

V

G

G

G

I

I

S

S

S
|
S

G

G

A

A

N

N

V

V

F

A

A

A

L

L

Q

Q

V

L

A

A

K

A

R

R

L

P

G

E

-

M

P

A

G

G

K

K

N

L

V

I

V

V

T
|
T

I

C

L

L

C

P

D

S

T

C

G

G

E

E

R

R

Y

Y

L

M

S

T

T

S

G

P

V

L

F

Y

P75 (= P72) mutation to L: In n5537; severe loss of protein stability.
A88 (≠ C85) mutation to V: In n5522; severe loss of protein stability.
S144 (≠ H140) mutation to F: In mr26; susceptible to high levels of hydrogen sulfide.
G181 (= G178) mutation to E: In n5521 and mr23; severe loss of protein stability. Susceptible to high levels of hydrogen sulfide.
G183 (= G180) mutation to R: In n5515; severe loss of protein stability.
G229 (= G226) mutation to E: In mr33; susceptible to high levels of hydrogen sulfide.
R259 (= R256) mutation to K: In n5519; no loss of protein stability. No effect on enzyme activity.
S272 (= S269) mutation to F: In mr29; susceptible to high levels of hydrogen sulfide.
T295 (= T291) mutation to I: In mr39; susceptible to high levels of hydrogen sulfide.

P0ABK5 Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine sulfhydrylase A; S-carboxymethylcysteine synthase; Sulfate starvation-induced protein 5; SSI5; EC 2.5.1.47; EC 4.5.1.5 from Escherichia coli (strain K12) (see 5 papers)
53% identity, 99% coverage: 3:306/307 of query aligns to 4:312/323 of P0ABK5

query
sites
P0ABK5

V

I

F

F

D

E

S

D

D

N

K

S

V

L

I

-

E

-

Q

T

I

I

G

G

N

H

T

T

P

P

L

L

Q

V

R

R

L

L

A

N

K

R

L

I

E

-

A

-

P

-

G

G

S

N

A

G

A

R

V

I

Y

L

G

A

K

K

A

V

E

E

F

S

F

R

N

N

P

P

G

S

G

F

S

S

V

V

K
|
K

D

C

R

R

I

I

C

G

L

A

A

N

M

M

I

I

E

W

A

D

A

A

E

E

K

K

D

R

G

G

S

V

L

L

K

K

P

P

G

G

M

V

T

E

V

L

V

V

E

E

P

P

T

T

S

S

G

G

N

N

T

T

G

G

I

I

G

A

L

L

S

A

M

Y

I

V

C

A

A

A

I

A

K

R

G

G

Y

Y

K

K

L

L

V

T

L

L

T

T

M

M

P

P

D

E

T

T

M

M

T

S

V

I

E

E

R

R

R

K

L

L

A

K

A

A

Y

L

G

G

A

A

E

N

L

L

V

V

L

L

T

T

P

E

G

G

A

A

Q

K

G

G

M

M

R

K

G

G

A

A

V

I

Q

Q

K

K

A

A

E

E

L

I

A

V

A

A

Q

S

-

N

-

P

P

E

G

K

H

Y

I

L

M

L

Q

Q

F

F

K

S

N

N

G

P

A

A

N

N

P

P

E

E

A

I

H

H

R

E

R

K

T

T

G

G

P

P

E

E

I

I

V

W

R

E

A

D

L

T

S

D

G

G

Q

Q

S

-

I

V

D

D

A

V

F

F

V

I

A

A

G

G

V

V

G

G

T

T

G

G

T

T

I

L

T

T

G

G

V

V

G

S

E

R

V

Y

L

I

K

K

E

G

H

T

N

K

A

G

A

K

V

T

R

D

I

L

-

I

-

S

V

V

A

A

V

V

E

E

P

P

D

T

A

D

S

S

P

P

I

V

L

I

S

A

G

Q

G

A

-

L

-

A

-

G

-

E

-

I

Q

K

P

P

G

G

P

P

H

H

K

K

I

I

Q

Q

G

G

I

I

G

G

A

A

G

G

F

F

V

I

P

P

D

A

V

N

L

L

N

D

T

L

K

K

V

L

Y

V

D

D

E

K

V

V

I

I

R

G

V

I

T

T

N

N

E

E

D

E

A

A

Y

I

E

S

T

T

V

A

R

R

L

L

A

M

Q

E

E

E

G

G

V

I

L

L

V

A

G

G

I

I

S

S

G

G

A

A

N

A

V

V

F

A

A

A

L

L

Q

K

V

L

A

Q

K

E

R

D

L

E

G

S

-

F

P

T

G

N

K

K

N

N

V

I

V

V

T

V

I

I

L

L

C

P

D

S

T

S

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

G

A

V

L

F

F

K42 (= K46) modified: N6-(pyridoxal phosphate)lysine; mutation to A: Still stimulates tRNase activity of CdiA-CT in vitro and in vivo.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

7n2tA O-acetylserine sulfhydrylase from citrullus vulgaris in the internal aldimine state, with citrate bound (see paper)
56% identity, 97% coverage: 9:307/307 of query aligns to 7:307/309 of 7n2tA

query
sites
7n2tA

V

V

I

T

E

E

Q

L

I

I

G

G

N

N

T

T

P

P

L

L

Q

V

R

Y

L

L

A

N

K

R

L

V

E

V

A

D

P

G

G

C

S

V

A

A

A

R

V

V

Y

A

G

A

K

K

A

L

E

E

F

M

N

E

P

P

G

C

G

S

S

S

V

V

K

K

D

D

R

R

I

I

C

G

L

Y

A

S

M

M

I

I

E

S

A

D

A

A

E

E

K

N

D

K

G

G

S

L

L

I

K

T

P

P

G

G

M

E

T

S

V

V

-

L

V

I

E

E

P

P

T

T

S

S

G

G

N

N

T

T

G

G

I

I

G

G

L

L

S

A

M

F

I

I

C

A

A

A

I

A

K

K

G

G

Y

Y

K

R

L

L

V

I

L

I

T

C

M

M

P

P

D

A

T

S

M

M

T

S

V

L

E

E

R

R

R

T

L

I

L

L

A

R

A

A

Y

F

G

G

A

A

E

E

L

L

V

V

L

L

T

T

P

D

G

P

A

A

Q

R

G

G

M

M

R

K

G

G

A

A

V

V

Q

Q

K

K

A

A

E

E

L

I

A

K

A

A

Q

K

-

T

P

P

G

N

H

S

I

Y

M

I

M

L

Q

Q

F

F

K

E

N

N

G

P

A

A

N

N

P

P

E

K

A

I

H

H

R

Y

R

E

T

T

G

G

P

P

E

E

I

I

V

W

R

R

A

G

L

-

S

S

G

G

Q

G

S

K

I

I

D

D

A

A

F

L

V

V

A

S

G

G

V

I

G

G

T

T

G

G

T

T

I

V

T

T

G

G

V

A

G

G

E

K

V

Y

L

L

K

K

E

E

H

Q

N

N

A

P

A

N

V

I

R

K

I

L

V

Y

A

G

V

V

E

E

P

P

D

V

A

E

S

S

P

A

I

I

L

L

S

S

G

G

Q

K

P

P

G

G

P

P

H

H

K

K

I

I

Q

Q

G

G

I

I

G

G

A

A

G

G

F

F

V

I

P

P

D

G

V

V

L

L

N

D

T

V

K

N

V

L

Y

L

D

D

E

E

V

V

I

I

R

Q

V

V

T

S

N

S

E

E

D

E

A

S

Y

I

E

E

T

T

V

A

R

K

R

L

L

L

A

A

Q

L

E

K

E

E

G

G

V

L

L

L

V

V

G

G

I

I

S

S

G

G

A

A

N

A

V

A

F

A

A

A

L

I

Q
x
R

V

I

A

A

K

K

R
|
R

-

P

L

E

G

N

P

A

G

G

K

K

N

L

V

I

V

V

T

A

I

V

L

F

C

P

D

S

T

F

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

G

V

V

L

F

F

D

E

binding phosphate ion: R277 (≠ Q278), R281 (= R282)

P0A1E3 Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine sulfhydrylase A; EC 2.5.1.47 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 4 papers)
53% identity, 96% coverage: 13:306/307 of query aligns to 12:312/323 of P0A1E3

query
sites
P0A1E3

I

I

G

G

N

H

T

T

P

P

L

L

Q

V

R

R

L

L

A

N

K

R

L

I

E

-

A

-

P

-

G

G

S

N

A

G

A

R

V

I

Y

L

G

A

K

K

A

V

E

E

F

S

F

R

N

N

P

P

G

S

G

F

S

S

V

V

K

K

D

C

R

R

I

I

C

G

L

A

A

N

M

M

I

I

E

W

A

D

A

A

E

E

K

K

D

R

G

G

S

V

L

L

K

K

P

P

G

G

M

V

T

E

V

L

V

V

E

E

P

P

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

G

A

L

L

S

A

M

Y

I

V

C

A

A

A

I

A

K

R

G

G

Y

Y

K

K

L

L

V

T

L

L

T

T

M

M

P

P

D

E

T

T

M

M

T

S

V

I

E

E

R

R

R

K

L

L

A

K

A

A

Y

L

G

G

A

A

E

N

L

L

V

V

L

L

T

T

P

E

G

G

A

A

Q

K

G

G

M

M

R

K

G

G

A

A

V

I

Q

Q

K

K

A

A

E

E

L

I

A

V

A

A

Q

S

P

D

-

P

-

Q

G

K

H

Y

I

L

M

L

Q

Q

F

F

K

S

N

N

G

P

A

A

N

N

P

P

E

E

A

I

H

H

R

E

R

K

T

T

G

G

P

P

E

E

I

I

V

W

R

E

A

D

L

T

S

D

G

G

Q

Q

S

-

I

V

D

D

A

V

F

F

V

I

A

S

G

G

V

V

G

G

T

T

G

G

T

T

I

L

T

T

G

G

V

V

G

T

E

R

V

Y

L

I

K

K

E

G

H

T

N

K

A

G

A

K

V

T

R

D

I

L

-

I

-

T

V

V

A

A

V

V

E

E

P

P

D

T

A

D

S

S

P

P

I

V

L

I

S

A

G

Q

G

A

-

L

-

A

-

G

-

E

-

I

Q

K

P

P

G

G

P

P

H

H

K

K

I

I

Q

Q

G

G

I

I

G

G

A

A

G

G

F

F

V

I

P

P

D

G

V

N

L

L

N

D

T

L

K

K

V

L

Y

I

D

D

E

K

V

V

I

V

R

G

V

I

T

T

N

N

E

E

D

E

A

A

Y

I

E

S

T

T

V

A

R

R

L

L

A

M

Q

E

E

E

G

G

V

I

L

L

V

A

G

G

I

I

S
|
S

S

S

G

G

A

A

N

A

V

V

F

A

A

A

L

L

Q

K

V

L

A

Q

K

E

R

D

L

E

G

S

-

F

P

T

G

N

K

K

N

N

V

I

V

V

T

V

I

I

L

L

C

P

D

S

T

S

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

G

A

V

L

F

F

N72 (= N76) binding pyridoxal 5'-phosphate
S273 (= S268) binding pyridoxal 5'-phosphate

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

4aecA Crystal structure of the arabidopsis thaliana o-acetyl-serine-(thiol)- lyasE C (see paper)
55% identity, 98% coverage: 6:306/307 of query aligns to 14:316/323 of 4aecA

query
sites
4aecA

S

A

D

D

K

N

V

V

I

S

E

Q

Q

L

I

I

G

G

N

K

T

T

P

P

L

M

Q

V

R

Y

L

L

A

N

K

S

L

I

E

A

A

K

P

G

G

C

S

V

A

A

A

N

V

I

Y

A

G

A

K

K

A

L

E

E

F

I

F

M

N

E

P

P

G

C

S

S

V

V

K
|
K

D

D

R

R

I

I

C

G

L

Y

A

S

M

M

I

V

E

T

A

D

A

A

E

E

K

Q

D

K

G

G

S

F

L

I

K

S

P

P

G

G

M

K

T

S

V

V

-

L

V

V

E

E

P

P

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

G

G

L

L

S

A

M

F

I

I

C

A

A

A

I

S

K

R

G

G

Y

Y

K

R

L

L

V

I

L

L

T

T

M

M

P

P

D

A

T

S

M

M

T

S

V

M

E

E

R

R

R

V

L

L

A

K

A

A

Y

F

G

G

A

A

E

E

L

L

V

V

L

L

T

T

P

D

G

P

A

A

Q

K

G

G

M

M

R

T

G

G

A

A

V

V

Q

Q

K

K

A

A

E

E

-

I

L

L

A

K

A

N

Q

T

P

P

G

D

H

A

I

Y

M

M

M

L

Q

Q

F

F

K

D

N

N

G

P

A

A

N

N

P

P

E

K

A

I

H

H

R

Y

R

E

T

T

G

G

P

P

E

E

I

I

V

W

R

D

A

D

L

T

S

K

G

G

Q

K

S

-

I

V

D

D

A

I

F

F

V

V

A

A

G

G

V
x
I

G
|
G

T
|
T

G
|
G

T
|
T

I

I

T

T

G

G

V

V

G

G

E

R

V

F

L

I

K

K

E

E

H

K

N

N

A

P

A

K

V

T

R

Q

I

V

V

I

A

G

V

V

E

E

P

P

D

T

A

E

S

S

P

D

I

I

L

L

S

S

G

G

Q

K

P

P

G

G

P

P

H

H

K

K

I

I

Q

Q

G
|
G

I

I

G

G

A

A

G

G

F

F

V

I

P

P

D

K

V

N

L

L

N

D

T

Q

K

K

V

I

Y

M

D

D

E

E

V

V

I

I

R

A

V

I

T

S

N

S

E

E

D

E

A

A

Y

I

E

E

T

T

V

A

R

K

R

Q

L

L

A

A

Q

L

E

K

E

E

G

G

V

L

L

M

V

V

G

G

I

I

S
|
S

S

S

G

G

A

A

N

A

V

A

F

A

A

A

L

I

Q

K

V

V

A

A

K

K

R

R

-

P

L

E

G

N

P

A

G

G

K

K

N

L

V

I

V

A

T

V

I

V

L

F

C
x
P

D

S

T

F

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

G

P

V

L

F

F

active site: K54 (= K46), S277 (= S268)
binding pyridoxal-5'-phosphate: K54 (= K46), N85 (= N76), I188 (≠ V179), G189 (= G180), T190 (= T181), G191 (= G182), G192 (= G183), T193 (= T184), G233 (= G224), S277 (= S268), P304 (≠ C294)

6z4nAAA structure of oass complexed with upar inhibitor (see paper)
53% identity, 96% coverage: 13:306/307 of query aligns to 13:313/321 of 6z4nAAA

query
sites
6z4nAAA

I

I

G

G

N

H

T

T

P

P

L

L

Q

V

R

R

L

L

A

N

K

R

L

I

E

-

A

-

P

-

G

G

S

N

A

G

A

R

V

I

Y

L

G

A

K

K

A

V

E

E

F

S

F

R

N

N

P

P

G

S

G

F

S

S

V

V

K
|
K

D

C

R

R

I

I

C

G

L

A

A

N

M

M

I

I

E

W

A

D

A

A

E

E

K

K

D

R

G

G

S

V

L

L

K

K

P

P

G

G

M

V

T

E

V

L

V

V

E

E

P

P

T
|
T

S

S

G
|
G

N
|
N

T
|
T

G

G

I

I

G

A

L

L

S

A

M

Y

I

V

C

A

A

A

I

A

K

R

G

G

Y

Y

K

K

L

L

V

T

L

L

T

T

M

M

P

P

D

E

T

T

M

M

T

S

V

I

E

E

R

R

R

K

L

L

A

K

A

A

Y

L

G

G

A

A

E

N

L

L

V

V

L

L

T

T

P

E

G

G

A

A

Q

K

G

G

M

M

R

K

G

G

A

A

V

I

Q

Q

K

K

A

A

E

E

L

I

A

V

A

A

Q

S

P

D

-

P

-

Q

G

K

H

Y

I

L

M

L

Q

Q

Q
|
Q

F
|
F

K

S

N

N

G

P

A

A

N

N

P

P

E

E

A

I

H

H

R

E

R

K

T

T

G

G

P

P

E

E

I

I

V

W

R

E

A

D

L

T

S

D

G

G

Q

Q

S

-

I

V

D

D

A

V

F

F

V

I

A

S

G

G

V
|
V

G
|
G

T
|
T

G
|
G

G

G

T
|
T

I

L

T

T

G

G

V

V

G

T

E

R

V

Y

L

I

K

K

E

G

H

T

N

K

A

G

A

K

V

T

R

D

I

L

-

I

-

T

V

V

A

A

V

V

E

E

P

P

D

T

A

D

S

S

P

P

I

V

L

I

S

A

G

Q

G

A

-

L

-

A

-

G

-

E

-

I

Q

K

P

P

G

G

P

P

H

H

K

K

I

I

Q
|
Q

G
|
G

I
|
I

G

G

A
|
A

G

G

F

F

V

I

P

P

D

G

V

N

L

L

N

D

T

L

K

K

V

L

Y

I

D

D

E

K

V

V

I

V

R

G

V

I

T

T

N

N

E

E

D

E

A

A

Y

I

E

S

T

T

V

A

R

R

L

L

A

M

Q

E

E

E

G

G

V

I

L

L

V

A

G

G

I

I

S
|
S

S

S

G

G

A

A

N

A

V

V

F

A

A

A

L

L

Q

K

V

L

A

Q

K

E

R

D

L

E

G

S

-

F

P

T

G

N

K

K

N

N

V

I

V

V

T

V

I

I

L

L

C
x
P

D

S

T

S

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

G

A

V

L

F

F

binding pyridoxal-5'-phosphate: K43 (= K46), N73 (= N76), V177 (= V179), G178 (= G180), T179 (= T181), G180 (= G182), T182 (= T184), G230 (= G224), S274 (= S268), P301 (≠ C294)
binding (1~{S},2~{S})-1-[(4-methylphenyl)methyl]-2-phenyl-cyclopropane-1-carboxylic acid: K43 (= K46), T70 (= T73), G72 (= G75), N73 (= N76), T74 (= T77), Q144 (= Q145), F145 (= F146), Q229 (= Q223), G230 (= G224), I231 (= I225), A233 (= A227)

2efyA Crystal structure of t.Th. Hb8 o-acetylserine sulfhydrylase complexed with 4-acetylbutyric acid
55% identity, 96% coverage: 13:306/307 of query aligns to 7:301/302 of 2efyA

query
sites
2efyA

I

I

G

G

N

K

T

T

P

P

L

V

Q

V

R

R

L

L

A

A

K

K

L

V

E

V

A

E

P

P

G

D

S

M

A

A

A

E

V

V

Y

W

G

V

K

K

A

L

E

E

F

G

F

L

N

N

P

P

G

G

S

S

V

I

K
|
K

D

D

R

R

I

P

C

A

L

W

A

Y

M

M

I

I

E

K

A

D

A

A

E

E

K

E

D

R

G

G

S

I

L

L

K

R

P

P

-

G

-

S

G

G

M

Q

T

V

V

I

V

V

E

E

P

P

T
|
T

S
|
S

G
|
G

N
|
N

T
|
T

G

G

I

I

G

G

L

L

S

A

M

M

I

I

C

A

A

A

I

S

K

R

G

G

Y

Y

K

R

L

L

V

I

L

L

T

T

M

M

P

P

D

A

T

Q

M

M

T

S

V

E

E

E

R

R

R

K

R

R

L

V

L

L

A

K

A

A

Y

F

G

G

A

A

E

E

L

L

V

V

L

L

T

T

P

D

G

P

A

E

Q

R

G

R

M

M

R

L

G

A

A

A

V

R

Q

E

K

E

A

A

E

L

E

R

L

L

A

K

A

E

Q

E

P

L

G

G

H

A

I

F

M

M

M

P

Q

D

Q
|
Q

F

F

K

K

N

N

G

P

A

A

N

N

P

V

E

R

A

A

H

H

R

Y

R

E

T

T

G

G

P

P

E

E

I

L

V

Y

R

E

A

A

L

L

S

E

G

G

Q

R

S

-

I

I

D

D

A

A

F

F

V

V

A
x
Y

G

G

V

S

G
|
G

T
|
T

G
|
G

G

G

T
|
T

I

I

T

T

G

G

V

V

G

G

E

R

V

Y

L

L

K

K

E

E

H

R

N

I

A

P

A

H

V

V

R

K

I

V

V

I

A

A

V

V

E
|
E

P

P

D

A

A

R

S
|
S

P

N

I

V

L

L

S

S

G

G

Q

K

P

M

G

G

P

Q

H

H

K

G

I

F

Q

Q

G
|
G

I
x
M

G

G

A
x
P

G

G

F

F

V

I

P

P

D

E

V

N

L

L

N

D

T

L

K

S

V

L

Y

L

D

D

E

G

V

V

I

I

R

Q

V

V

T

W

N

E

E

E

D

D

A

A

Y

F

E

P

T

L

V

A

R

R

L

L

A

A

Q

R

E

E

G

G

V

L

L

F

V

L

G

G

I

M

S
|
S

S

S

G

G

A

G

N

I

V

V

F

W

A

A

L

L

Q

Q

V

V

A

A

K

R

R

E

L

L

G

G

P

P

G

G

K

K

N

R

V

V

V

A

T

C

I

I

L

S

C
x
P

D
|
D

T

G

G

G

E

W

R

K

Y

Y

L

L

S

S

T

T

G

P

V

L

F

Y

active site: K40 (= K46), S70 (= S74), E200 (= E205), S204 (= S209), S263 (= S268)
binding 5-oxohexanoic acid: T69 (= T73), G71 (= G75), T73 (= T77), Q141 (= Q145), G175 (= G180), G219 (= G224), M220 (≠ I225), P222 (≠ A227)
binding pyridoxal-5'-phosphate: K40 (= K46), N72 (= N76), Y172 (≠ A177), G175 (= G180), T176 (= T181), G177 (= G182), T179 (= T184), G219 (= G224), S263 (= S268), P289 (≠ C294), D290 (= D295)

2ecqA Crystal structure of t.Th. Hb8 o-acetylserine sulfhydrylase complexed with 3-hydroxylactate
55% identity, 96% coverage: 13:306/307 of query aligns to 7:301/302 of 2ecqA

query
sites
2ecqA

I

I

G

G

N

K

T

T

P

P

L

V

Q

V

R

R

L

L

A

A

K

K

L

V

E

V

A

E

P

P

G

D

S

M

A

A

A

E

V

V

Y

W

G

V

K

K

A

L

E

E

F

G

F

L

N

N

P

P

G

G

S

S

V

I

K
|
K

D

D

R

R

I

P

C

A

L

W

A

Y

M

M

I

I

E

K

A

D

A

A

E

E

K

E

D

R

G

G

S

I

L

L

K

R

P

P

-

G

-

S

G

G

M

Q

T

V

V

I

V

V

E

E

P

P

T

T

S
|
S

G
|
G

N
|
N

T
|
T

G

G

I

I

G

G

L

L

S

A

M

M

I

I

C

A

A

A

I

S

K

R

G

G

Y

Y

K

R

L

L

V

I

L

L

T

T

M

M

P

P

D

A

T

Q

M

M

T

S

V

E

E

E

R

R

R

K

R

R

L

V

L

L

A

K

A

A

Y

F

G

G

A

A

E

E

L

L

V

V

L

L

T

T

P

D

G

P

A

E

Q

R

G

R

M

M

R

L

G

A

A

A

V

R

Q

E

K

E

A

A

E

L

E

R

L

L

A

K

A

E

Q

E

P

L

G

G

H

A

I

F

M

M

M

P

Q

D

Q
|
Q

F

F

K

K

N

N

G

P

A

A

N

N

P

V

E

R

A

A

H

H

R

Y

R

E

T

T

G

G

P

P

E

E

I

L

V

Y

R

E

A

A

L

L

S

E

G

G

Q

R

S

-

I

I

D

D

A

A

F

F

V

V

A
x
Y

G
|
G

V

S

G
|
G

T
|
T

G

G

T
|
T

I

I

T

T

G

G

V

V

G

G

E

R

V

Y

L

L

K

K

E

E

H

R

N

I

A

P

A

H

V

V

R

K

I

V

V

I

A

A

V

V

E
|
E

P

P

D

A

A

R

S
|
S

P

N

I

V

L

L

S

S

G

G

Q

K

P

M

G

G

P

Q

H

H

K

G

I

F

Q

Q

G
|
G

I

M

G

G

A

P

G

G

F

F

V

I

P

P

D

E

V

N

L

L

N

D

T

L

K

S

V

L

Y

L

D

D

E

G

V

V

I

I

R

Q

V

V

T

W

N

E

E

E

D

D

A

A

Y

F

E

P

T

L

V

A

R

R

L

L

A

A

Q

R

E

E

G

G

V

L

L

F

V

L

G

G

I

M

S
|
S

S

S

G

G

A

G

N

I

V

V

F

W

A

A

L

L

Q

Q

V

V

A

A

K

R

R

E

L

L

G

G

P

P

G

G

K

K

N

R

V

V

V

A

T

C

I

I

L

S

C
x
P

D

D

T

G

G

G

E

W

R

K

Y

Y

L

L

S

S

T

T

G

P

V

L

F

Y

active site: K40 (= K46), S70 (= S74), E200 (= E205), S204 (= S209), S263 (= S268)
binding (3s)-3-hydroxybutanoic acid: K40 (= K46), G71 (= G75), T73 (= T77), Q141 (= Q145), G219 (= G224)
binding pyridoxal-5'-phosphate: K40 (= K46), N72 (= N76), Y172 (≠ A177), G173 (= G178), G175 (= G180), T176 (= T181), T179 (= T184), G219 (= G224), S263 (= S268), P289 (≠ C294)

2ecoA Crystal structure of t.Th. Hb8 o-acetylserine sulfhydrylase complexed with 4-methylvalerate
55% identity, 96% coverage: 13:306/307 of query aligns to 7:301/302 of 2ecoA

query
sites
2ecoA

I

I

G

G

N

K

T

T

P

P

L

V

Q

V

R

R

L

L

A

A

K

K

L

V

E

V

A

E

P

P

G

D

S

M

A

A

A

E

V

V

Y

W

G

V

K

K

A

L

E

E

F

G

F

L

N

N

P

P

G

G

S

S

V

I

K
|
K

D

D

R

R

I

P

C

A

L

W

A

Y

M

M

I

I

E

K

A

D

A

A

E

E

K

E

D

R

G

G

S

I

L

L

K

R

P

P

-

G

-

S

G

G

M

Q

T

V

V

I

V

V

E

E

P

P

T
|
T

S
|
S

G
|
G

N
|
N

T
|
T

G

G

I

I

G

G

L

L

S

A

M

M

I

I

C

A

A

A

I

S

K

R

G

G

Y

Y

K

R

L

L

V

I

L

L

T

T

M

M

P

P

D

A

T

Q

M

M

T

S

V

E

E

E

R

R

R

K

R

R

L

V

L

L

A

K

A

A

Y

F

G

G

A

A

E

E

L

L

V

V

L

L

T

T

P

D

G

P

A

E

Q

R

G

R

M

M

R

L

G

A

A

A

V

R

Q

E

K

E

A

A

E

L

E

R

L

L

A

K

A

E

Q

E

P

L

G

G

H

A

I

F

M

M

M

P

Q

D

Q
|
Q

F

F

K

K

N

N

G

P

A

A

N

N

P

V

E

R

A

A

H

H

R

Y

R

E

T

T

G

G

P

P

E

E

I

L

V

Y

R

E

A

A

L

L

S

E

G

G

Q

R

S

-

I

I

D

D

A

A

F

F

V

V

A
x
Y

G

G

V

S

G
|
G

T
|
T

G

G

T
|
T

I

I

T

T

G

G

V

V

G

G

E

R

V

Y

L

L

K

K

E

E

H

R

N

I

A

P

A

H

V

V

R

K

I

V

V

I

A

A

V

V

E
|
E

P

P

D

A

A

R

S
|
S

P

N

I

V

L

L

S

S

G

G

Q

K

P

M

G

G

P

Q

H

H

K

G

I

F

Q

Q

G
|
G

I

M

G

G

A

P

G

G

F

F

V

I

P

P

D

E

V

N

L

L

N

D

T

L

K

S

V

L

Y

L

D

D

E

G

V

V

I

I

R

Q

V

V

T

W

N

E

E

E

D

D

A

A

Y

F

E

P

T

L

V

A

R

R

L

L

A

A

Q

R

E

E

G

G

V

L

L

F

V

L

G

G

I

M

S
|
S

S

S

G

G

A

G

N

I

V

V

F

W

A

A

L

L

Q

Q

V

V

A

A

K

R

R

E

L

L

G

G

P

P

G

G

K

K

N

R

V

V

V

A

T

C

I

I

L

S

C
x
P

D
|
D

T

G

G

G

E

W

R

K

Y

Y

L

L

S

S

T

T

G

P

V

L

F

Y

active site: K40 (= K46), S70 (= S74), E200 (= E205), S204 (= S209), S263 (= S268)
binding 4-methyl valeric acid: K40 (= K46), T69 (= T73), G71 (= G75), T73 (= T77), Q141 (= Q145), G175 (= G180), T176 (= T181), G219 (= G224)
binding pyridoxal-5'-phosphate: K40 (= K46), N72 (= N76), Y172 (≠ A177), G175 (= G180), T176 (= T181), T179 (= T184), G219 (= G224), S263 (= S268), P289 (≠ C294), D290 (= D295)

1d6sA Crystal structure of the k41a mutant of o-acetylserine sulfhydrylase complexed in external aldimine linkage with methionine (see paper)
53% identity, 96% coverage: 13:306/307 of query aligns to 11:311/322 of 1d6sA

query
sites
1d6sA

I

I

G

G

N

H

T

T

P

P

L

L

Q

V

R

R

L

L

A

N

K

R

L

I

E

-

A

-

P

-

G

G

S

N

A

G

A

R

V

I

Y

L

G

A

K

K

A

V

E

E

F

S

F

R

N

N

P

P

G

S

G

F

S

S

V

V

K
x
A

D

C

R

R

I

I

C

G

L

A

A

N

M

M

I

I

E

W

A

D

A

A

E

E

K

K

D

R

G

G

S

V

L

L

K

K

P

P

G

G

M

V

T

E

V

L

V

V

E

E

P

P

T
|
T

S
x
N

G

G

N
|
N

T
|
T

G

G

I

I

G

A

L

L

S

A

M

Y

I

V

C

A

A

A

I

A

K

R

G

G

Y

Y

K

K

L

L

V

T

L

L

T

T

M

M

P

P

D

E

T

T

M

M

T

S

V

I

E

E

R

R

R

K

L

L

A

K

A

A

Y

L

G

G

A

A

E

N

L

L

V

V

L

L

T

T

P

E

G

G

A

A

Q

K

G

G

M

M

R

K

G

G

A

A

V

I

Q

Q

K

K

A

A

E

E

L

I

A

V

A

A

Q

S

P

D

-

P

-

Q

G

K

H

Y

I

L

M

L

Q

Q

Q
|
Q

F
|
F

K

S

N

N

G

P

A

A

N

N

P

P

E

E

A

I

H

H

R

E

R

K

T

T

G

G

P

P

E

E

I

I

V

W

R

E

A

D

L

T

S

D

G

G

Q

Q

S

-

I

V

D

D

A

V

F

F

V

I

A

S

G

G

V

V

G
|
G

T
|
T

G
|
G

G

G

T
|
T

I

L

T

T

G

G

V

V

G

T

E

R

V

Y

L

I

K

K

E

G

H

T

N

K

A

G

A

K

V

T

R

D

I

L

-

I

-

T

V

V

A

A

V

V

E

E

P

P

D

T

A

D

S

S

P

P

I

V

L

I

S

A

G

Q

G

A

-

L

-

A

-

G

-

E

-

I

Q

K

P

P

G

G

P

P

H

H

K

K

I

I

Q

Q

G
|
G

I

I

G

G

A

A

G

G

F

F

V

I

P

P

D

G

V

N

L

L

N

D

T

L

K

K

V

L

Y

I

D

D

E

K

V

V

I

V

R

G

V

I

T

T

N

N

E

E

D

E

A

A

Y

I

E

S

T

T

V

A

R

R

L

L

A

M

Q

E

E

E

G

G

V

I

L

L

V

A

G

G

I

I

S
|
S

S

S

G

G

A

A

N

A

V

V

F

A

A

A

L

L

Q

K

V

L

A

Q

K

E

R

D

L

E

G

S

-

F

P

T

G

N

K

K

N

N

V

I

V

V

T

V

I

I

L

L

C
x
P

D

S

T

S

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

G

A

V

L

F

F

active site: A41 (≠ K46), G228 (= G224)
binding methionine: T68 (= T73), N69 (≠ S74), N71 (= N76), T72 (= T77), Q142 (= Q145), F143 (= F146), G176 (= G180), G228 (= G224)
binding pyridoxal-5'-phosphate: N71 (= N76), G176 (= G180), T177 (= T181), G178 (= G182), T180 (= T184), G228 (= G224), S272 (= S268), P299 (≠ C294)

P47999 Cysteine synthase, chloroplastic/chromoplastic; At.OAS.7-4; Beta-substituted Ala synthase 2;1; ARAth-Bsas2;1; CSase B; AtCS-B; CS-B; O-acetylserine (thiol)-lyase; O-acetylserine sulfhydrylase; OAS-TL B; cpACS1; EC 2.5.1.47 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
55% identity, 98% coverage: 6:307/307 of query aligns to 76:379/392 of P47999

query
sites
P47999

S

A

D

D

K

N

V

A

I

A

E

Q

Q

L

I

I

G

G

N

K

T

T

P

P

L

M

Q

V

R

Y

L

L

A

N

K

N

L

V

E

V

A

K

P

G

G

C

S

V

A

A

A

S

V

V

Y

A

G

A

K

K

A

L

E

E

F

I

F

M

N

E

P

P

G

C

S

S

V

V

K

K

D

D

R

R

I

I

C

G

L

Y

A

S

M

M

I

I

E

T

A

D

A

A

E

E

K

E

D

K

G

G

S

L

L

I

K

T

P

P

G

G

M

K

T

S

V

V

-

L

V

V

E

E

P

S

T

T

S

S

G

G

N

N

T

T

G

G

I

I

G

G

L

L

S

A

M

F

I

I

C

A

A

A

I

S

K

K

G

G

Y

Y

K

K

L

L

V

I

L

L

T

T

M

M

P

P

D

A

T

S

M

M

T

S

V

L

E

E

R

R

R

V

L

L

A

R

A

A

Y

F

G

G

A

A

E

E

L

L

V

V

L

L

T

T

P

E

G

P

A

A

Q

K

G

G

M

M

R

T

G

G

A

A

V

I

Q

Q

K

K

A

A

E

E

-

I

L

L

A

K

Q

T

P

P

G

N

H

S

I

Y

M

M

M

L

Q

Q

F

F

K

D

N

N

G

P

A

A

N

N

P

P

E

K

A

I

H

H

R

Y

R

E

T

T

G

G

P

P

E

E

I

I

V

W

R

E

A

D

L

T

S

R

G

G

Q

K

S

-

I

I

D

D

A

I

F

L

V

V

A

A

G

G

V

I

G

G

T

T

G

G

T

T

I

I

T

T

G

G

V

V

G

G

E

R

V

F

L

I

K

K

E

E

H

R

N

K

A

P

A

E

V

L

R

K

I

V

V

I

A

G

V

V

E

E

P

P

D

T

A

E

S

S

P

A

I

I

L

L

S

S

G

G

Q

K

P

P

G

G

P

P

H

H

K

K

I

I

Q

Q

G

G

I

I

G

G

A

A

G

G

F

F

V

V

P

P

D

K

V

N

L

L

N

D

T

L

K

A

V

I

Y

V

D

D

E

E

V

Y

I

I

R

A

V

I

T

S

N

S

E

E

D

E

A

A

Y

I

E

E

T

T

V

S

R

K

R

Q

L

L

A

A

Q

L

E

Q

E

E

G

G

V

L

L

L

V

V

G

G

I

I

S

S

G

G

A

A

N

A

V

A

F

A

A

A

L

I

Q

Q

V

V

A

A

K

K

R

R

-

P

L

E

G

N

P

A

G

G

K

K

N

L

V

I

V

A

T

V

I

V

L

F

C

P

D

S

T

F

G

G

E

E

R

R

Y

Y

L

L

S

S

T

T

G

Q

V

L

F

F

D

Q

Sites not aligning to the query:

61 modified: N-acetylalanine

Query Sequence

>WP_004514479.1 NCBI__GCF_000012925.1:WP_004514479.1
MPVFDSDKVIEQIGNTPLQRLAKLEAPGSAAVYGKAEFFNPGGSVKDRICLAMIEAAEKD
GSLKPGMTVVEPTSGNTGIGLSMICAIKGYKLVLTMPDTMTVERRRLLAAYGAELVLTPG
AQGMRGAVQKAEELAAQPGHIMMQQFKNGANPEAHRRTTGPEIVRALSGQSIDAFVAGVG
TGGTITGVGEVLKEHNAAVRIVAVEPDASPILSGGQPGPHKIQGIGAGFVPDVLNTKVYD
EVIRVTNEDAYETVRRLAQEEGVLVGISSGANVFAALQVAKRLGPGKNVVTILCDTGERY
LSTGVFD

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory