SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_004514768.1 NCBI__GCF_000012925.1:WP_004514768.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

7kb1C Complex of o-acety-l-homoserine aminocarboxypropyltransferase (mety) from thermotoga maritima and a key reaction intermediate (see paper)
65% identity, 99% coverage: 5:427/428 of query aligns to 4:427/428 of 7kb1C

query
sites
7kb1C

K

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binding pyridoxal-5'-phosphate: Y57 (= Y57), R59 (= R59)
binding (2E)-2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)imino]but-3-enoic acid: G87 (= G87), Q88 (= Q88), Y112 (= Y112), N160 (= N159), D185 (= D184), S206 (= S206), T208 (= T208), K209 (= K209), N369 (= N369), I370 (= I370), R404 (= R404)

7kb1A Complex of o-acety-l-homoserine aminocarboxypropyltransferase (mety) from thermotoga maritima and a key reaction intermediate (see paper)
65% identity, 99% coverage: 5:427/428 of query aligns to 4:427/428 of 7kb1A

query
sites
7kb1A

K

K

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Y

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N

T

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A

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E

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Y

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binding (2E)-2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)imino]but-3-enoic acid: Y57 (= Y57), R59 (= R59), G87 (= G87), Q88 (= Q88), Y112 (= Y112), N160 (= N159), D185 (= D184), S206 (= S206), T208 (= T208), K209 (= K209), N369 (= N369), I370 (= I370), R404 (= R404)

2ctzA Crystal structure of o-acetyl homoserine sulfhydrylase from thermus thermophilus hb8
56% identity, 98% coverage: 6:424/428 of query aligns to 1:421/421 of 2ctzA

query
sites
2ctzA

I

M

S

R

F

F

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E

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A

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L

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H

A

A

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Y

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S

Y

Y

A

V

F

F

K

K

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S

P

E

E

H

H

A

A

A

A

N

N

L

L

F

F

G

A

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L

K

K

E

E

F

F

G

G

N

N

I

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Y

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L

I

M

M

N

N

P

P

T

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D

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G

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x
H

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x
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A

A

V

L

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T

N

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A

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Q

A

A

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G

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D

N

N

I

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Y

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Y

G

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F

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D

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G

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N

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A

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G

V

V

T

S

Q

P

D

E

Y

M

I

V

R

R

L

L

S

S

I

V

G

G

I

L

E

E

E

H

V

V

A

E

D

D

I

L

I

K

A

A

D

E

I

L

D

K

Q

E

A

A

L

L

A

A

active site: R54 (= R59), Y107 (= Y112), D180 (= D184), K206 (= K209)
binding pyridoxal-5'-phosphate: S81 (= S86), G82 (= G87), H83 (≠ Q88), Q86 (≠ I91), Y107 (= Y112), D180 (= D184), T182 (= T186), S203 (= S206), T205 (= T208), K206 (= K209)

Q5SK88 O-acetyl-L-homoserine sulfhydrylase 1; OAH-sulfhydrylase 1; EC 2.5.1.- from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)
56% identity, 98% coverage: 6:424/428 of query aligns to 1:421/421 of Q5SK88

query
sites
Q5SK88

I

M

S

R

F

F

D

E

T

T

L

L

A

Q

L

L

H

H

A

A

G

G

Q

Y

E

E

P

P

D

E

P

P

T

T

T

T

L

L

S

S

R

R

A

Q

V

V

P

P

I

I

Y

Y

Q

P

T

T

A

T

S

S

Y

Y

A

V

F

F

K

K

S

S

S

P

E

E

H

H

A

A

A

A

N

N

L

L

F

F

G

A

L

L

K

K

E

E

F

F

G

G

N

N

I

I

Y

Y

T

S

R

R

L

I

M

M

N

N

P

P

T

T

S

V

D

D

V

V

L

L

E

E

K

K

R

R

L

L

A

A

E

A

L

L

D

E

G

G

G

G

V

K

G

A

A

A

L

L

A

A

V

T

A

A

S

S

G

G

Q

H

A

A

A

A

I

Q

T

F

Y

L

A

A

V

L

L

T

N

T

I

L

A

A

Q

Q

A

A

G

G

Q

D

N

N

I

I

V

V

S

S

T

T

S

P

Y

N

L

L

Y

Y

G

G

G

G

T

T

Y

F

N

N

L

Q

F

F

H

K

Y

V

T

T

L

L

P

K

K

R

L

L

G

G

I

I

T

E

V

V

K

R

F

F

V

T

D

S

T

R

S

E

D

E

-

R

P

P

E

E

N

E

V

F

R

L

R

A

A

L

I

T

D

D

D

E

K

K

T

T

R

R

L

A

V

W

Y

W

T

V

E

E

S

S

V

I

G

G

N

N

P

P

K

A

N

L

N

N

V

I

D

P

D

D

F

L

E

E

A

A

I

L

A

A

R

Q

I

A

A

A

H

R

E

E

A

K

G

G

I

V

P

A

F

L

V

I

V

V

D

D

N

N

T

T

V

F

T

G

T

M

S

G

-

G

Y

Y

L

L

F

L

K

R

P

P

L

L

E

A

H

W

G

G

A

A

D

A

I

L

V

V

V

T

Y

H

S

S

L

L

T

T

K
|
K

F

W

I

V

G

G

G

G

H

H

G

G

T

A

S

V

I

I

G

A

G

G

A

A

V

I

V

V

D

D

G

G

G

G

K

N

F

F

P

P

W

W

N

E

N

G

G

G

R

R

F

Y

P

P

E

L

M

L

T

T

E

E

P

P

D

Q

P

P

S

G

Y

Y

H

H

G

G

L

L

C

R

Y

L

W

T

E

E

A

A

L

F

G

G

N

E

L

L

S

A

Y

F

I

I

L

V

K

K

M

A

R

R

I

V

T

D

L

G

L

L

R

R

D

D

M

Q

G

G

A

Q

C

A

I

L

S

G

P

P

F

F

N

E

S

A

F

W

L

V

F

V

L

L

Q

L

G

G

L

M

E

E

T

T

L

L

P

S

V

L

R

R

M

A

A

E

R

R

H

H

V

V

E

E

N

N

A

T

R

L

A

H

V

L

A

A

Q

H

W

W

L

L

E

L

K

E

H

Q

P

P

L

Q

V

V

S

A

W

W

V

V

N

N

Y

Y

P

P

G

G

L

L

A

P

S

H

H

H

K

P

D

H

H

H

G

D

N

R

A

A

R

Q

K

K

Y

Y

L

F

P

K

K

G

G

K

E

P

G

G

A

A

I

V

I

L

G

T

F

F

G

G

I

L

N

K

G

G

G

G

L

Y

E

E

A

A

G

A

R

K

R

R

F

F

I

I

D

S

S

R

A

L

K

K

L

L

L

I

S

S

H

H

L

L

A

A

N

N

I

V

G

G

D

D

A

T

K

R

S

T

L

L

V

A

I

I

H

H

P

P

A

A

S

S

T

T

T

T

H

H

Q

S

Q

Q

L

L

S

S

E

P

E

E

E

E

Q

Q

L

A

A

Q

S

A

G

G

V

V

T

S

Q

P

D

E

Y

M

I

V

R

R

L

L

S

S

I

V

G

G

I

L

E

E

E

H

V

V

A

E

D

D

I

L

I

K

A

A

D

E

I

L

D

K

Q

E

A

A

L

L

A

A

K206 (= K209) modified: N6-(pyridoxal phosphate)lysine

O13326 Homocysteine synthase; O-acetylhomoserine sulfhydrylase; OAH SHL; OAH sulfhydrylase; EC 2.5.1.49 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
58% identity, 97% coverage: 8:423/428 of query aligns to 8:426/429 of O13326

query
sites
O13326

F

F

D

E

T

T

L

L

A

Q

L

L

H

H

A

A

G

G

Q

Q

E

E

P

P

D

D

P

A

T

A

T

T

L

S

S

S

R

R

A

A

V

V

P

P

I

I

Y

Y

Q

A

T

T

A

T

S

S

Y

Y

A

V

F

F

K

R

S

D

S

C

E

D

H

H

A

G

A

G

N

R

L

L

F

F

G

G

L

L

K

Q

E

E

F

P

G

G

N

Y

I

I

Y

Y

T

S

R

R

L

M

M

M

N

N

P

P

T

T

S

A

D

D

V

V

L

F

E

E

K

K

R

R

L

I

A

A

E

A

L

L

D

E

G

H

G

G

V

A

G

A

A

A

L

I

A

A

V

T

A

S

S

S

G

G

Q

T

A

S

A

A

I

L

T

F

Y

M

A

A

V

L

L

T

N

T

I

L

A

A

Q

K

A

A

G

G

Q

D

N

N

I

I

V

V

S

S

T

T

S

S

Y

Y

L

L

Y

Y

G

G

G

G

T

T

Y

Y

N

N

L

L

F

F

H

K

Y

V

T

T

L

L

P

P

K

R

L

L

G

G

I

I

T

T

V

T

K

K

F

F

V

V

D

N

T

G

S

D

D

D

P

P

E

N

N

D

V

L

R

A

R

A

A

Q

I

I

D

D

D

E

K

N

T

T

R

K

L

A

V

V

Y

Y

T

V

E

E

S

S

V

I

G

G

N

N

P

P

K

M

N

Y

N

N

V

V

D

P

D

D

F

F

E

E

A

R

I

I

A

A

R

E

I

V

A

A

H

H

E

A

A

A

G

G

I

V

P

P

F

L

V

M

V

V

D

D

N

N

T

T

V

F

-

G

T

G

T

G

S

G

Y

Y

L

L

F

V

K

R

P

P

L

I

E

D

H

H

G

G

A

A

D

D

I

I

V

V

V

T

Y

H

S

S

L

A

T

T

K

K

F

W

I

I

G

G

G

G

H

H

G

G

T

T

S

T

I

I

G

G

G

G

A

V

V

I

V

V

D

D

G

S

G

G

K

K

F

F

P

D

W

W

-

K

-

K

N

N

N

S

G

K

R

R

F

F

P

P

E

E

M

F

T

N

E

E

P

P

D

H

P

P

S

G

Y

Y

H

H

G

G

L

M

C

V

Y

F

W

T

E

E

A

T

L

F

G

G

N

N

L

L

S

A

Y

Y

I

A

L

F

K

A

M

C

R

R

I

T

T

Q

L

T

L

L

R

R

D

D

M

V

G

G

A

G

C

N

I

A

S

N

P

P

F

F

N

G

S

V

F

F

L

L

F

L

L

L

Q

Q

G

G

L

L

E

E

T

T

L

L

P

S

V

L

R

R

M

M

A

E

R

R

H

H

V

V

E

Q

N

N

A

A

R

F

A

A

V

L

A

A

Q

K

W

Y

L

L

E

E

K

K

H

H

P

P

L

K

V

V

S

N

W

W

V

V

N

S

Y

Y

P

P

G

G

L

L

A

E

S

S

H

H

K

V

D

S

H

H

G

K

N

L

A

A

R

K

K

K

Y

Y

L

L

P

K

K

N

G

G

E

Y

G

G

A

A

I

V

I

L

G

S

F

F

G

G

I

A

N

K

G

G

G

G

L

P

E

D

A

Q

G

S

R

R

R

K

F

V

I

V

D

N

S

A

A

L

K

K

L

L

L

A

S

S

H

Q

L

L

A

A

N

N

I

V

G

G

D

D

A

A

K

K

S

T

L

L

V

V

I

I

H

A

P

P

A

A

S

Y

T

T

T

T

H

H

Q

L

Q

Q

L

L

S

T

E

D

E

E

E

E

Q

Q

L

I

A

S

S

A

G

G

V

V

T

T

Q

K

D

D

Y

L

I

I

R

R

L

V

S

A

I

V

G
|
G

I

I

E

E

E

H

V

I

A

D

D

D

I

I

I

I

A

A

D

D

I

F

D

A

Q

Q

A

A

L

L

G411 (= G408) mutation to D: Impairs homocysteine synthase activity.

8erbK Crystal structure of fub7 in complex with vinylglycine ketimine (see paper)
56% identity, 99% coverage: 7:428/428 of query aligns to 5:429/429 of 8erbK

query
sites
8erbK

S

N

F

F

D

E

T

T

L

L

A

Q

L

L

H

H

A

A

G

G

Q

Y

E

T

P

P

D

D

P

P

T

H

T

T

L

R

S

S

R

T

A

A

V

V

P

P

I

I

Y

Y

Q

A

T

T

A

S

S

S

Y

Y

A

T

F

F

K

N

S

D

S

S

E

A

H

H

A

G

A

A

N

R

L

L

F

F

G

G

L

L

K

K

E

E

F

L

G

G

N

N

I

I

Y
|
Y

T

S

R
|
R

L

L

M

M

N

N

P

P

T

T

S

V

D

D

V

V

L

F

E

E

K

K

R

R

L

I

A

A

E

A

L

L

D

E

G

G

G

G

V

I

G

A

A

A

L

A

A

A

V

T

A

S

S

S

G
|
G

Q
|
Q

A

A

A

A

I
x
Q

T

F

Y

L

A

T

V

I

L

A

N

T

I

L

A

A

Q

K

A

A

G

G

Q

D

N

N

I

I

V

V

S

A

T

S

S

S

Y

H

L

L

Y
|
Y

G

G

G

G

T

T

Y

Y

N

N

L

Q

F

L

H

N

Y

V

T

L

L

L

P

P

K

R

L

F

G

G

I

I

T

K

V

T

K

K

F

F

V

V

D

R

T

S

S

G

D

K

P

L

E

E

N

D

V

Y

R

A

R

A

A

A

I

I

D

D

D

D

K

Q

T

T

R

R

L

A

V

I

Y

Y

T

V

E

E

S

S

V

M

G

S

N
|
N

P

P

K

D

N

Y

N

V

V

V

D

P

D

D

F

F

E

E

A

G

I

I

A

A

R

K

I

I

A

A

H

H

E

E

A

H

G

G

I

I

P

P

F

L

V

V

V

V

D
|
D

N

N

T

T

V

L

T

G

T

A

S

G

-

G

Y

Y

L

Y

F

I

K

R

P

P

L

I

E

E

H

H

G

G

A

A

D

D

I

I

V

V

V

V

Y

H

S
|
S

L

A

T
|
T

K
|
K

F

W

I

I

G

G

G

G

H

H

G

G

T

T

S

T

I

I

G

G

G

G

A

V

V

I

V

V

D

D

G

S

G

G

K

R

F

F

P

N

W

W

N

N

-

K

-

H

N

S

G

D

R

R

F

F

P

P

E

E

M

M

T

V

E

E

P

P

D

S

P

P

S

S

Y

Y

H

H

G

G

L

L

C

K

Y

Y

W

W

E

E

A

A

L

F

G

G

N

P

L

A

S

T

Y

F

I

I

L

T

K

R

M

I

R

R

I

V

T

E

L

M

L

L

R

R

D

D

M

I

G

G

A

A

C

C

I

L

S

S

P

P

F

F

N

S

S

A

F

Q

L

Q

F

L

L

L

Q

L

G

G

L

I

E

E

T

T

L

L

P

G

V

L

R

R

M

A

A

E

R

R

H

H

V

A

E

Q

N

N

A

T

R

E

A

K

V

L

A

S

Q

K

W

Y

L

F

E

E

K

S

H

S

P

P

L

N

V

V

S

S

W

W

V

V

N

L

Y

W

P

P

G

G

L

S

A

E

S

S

H

H

K

P

D

T

H

Y

G

S

N

Q

A

A

R

K

K

K

Y

Y

L

L

P

T

K

R

G

G

E

F

G

G

A

A

I

M

I

L

G

S

F

I

G

G

I

V

N

K

G

G

G

D

L

A

E

S

A

A

G

G

R

S

R

K

F

V

I

V

D

D

S

G

A

L

K

K

L

L

L

V

S

S

H

N

L

L

A

A

N

N

I

V

G

G

D

D

A

A

K

K

S

S

L

L

V

A

I

I

H

H

P

P

A

W

S

S

T

T

T
|
T

H

H

Q

E

Q

Q

L

L

S

S

E

E

E

D

E

E

Q

R

L

L

A

A

S

S

G

G

V

V

T

T

Q

E

D

D

Y

M

I

I

R
|
R

L

I

S

S

I

V

G

G

I

I

E

E

E

H

V

V

A

D

D

D

I

I

I

I

A

A

D

D

I

F

D

E

Q

Q

A

S

L

F

A

Q

A

K

S

A

Q

Y

G

G

binding (2E)-2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)imino]but-3-enoic acid: Y55 (= Y57), R57 (= R59), G85 (= G87), Q86 (= Q88), Q89 (≠ I91), Y110 (= Y112), N157 (= N159), D182 (= D184), S205 (= S206), T207 (= T208), K208 (= K209), T385 (= T384), R405 (= R404)

8erjB Crystal structure of fub7 in complex with e-2-aminocrotonate (see paper)
56% identity, 99% coverage: 7:428/428 of query aligns to 4:428/428 of 8erjB

query
sites
8erjB

S

N

F

F

D

E

T

T

L

L

A

Q

L

L

H

H

A

A

G

G

Q

Y

E

T

P

P

D

D

P

P

T

H

T

T

L

R

S

S

R

T

A

A

V

V

P

P

I

I

Y

Y

Q

A

T

T

A

S

S

S

Y

Y

A

T

F

F

K

N

S

D

S

S

E

A

H

H

A

G

A

A

N

R

L

L

F

F

G

G

L

L

K

K

E

E

F

L

G

G

N

N

I

I

Y

Y

T

S

R

R

L

L

M

M

N

N

P

P

T

T

S

V

D

D

V

V

L

F

E

E

K

K

R

R

L

I

A

A

E

A

L

L

D

E

G

G

G

G

V

I

G

A

A

A

L

A

A

A

V

T

A

S

S

S

G
|
G

Q
|
Q

A

A

A

A

I
x
Q

T

F

Y

L

A

T

V

I

L

A

N

T

I

L

A

A

Q

K

A

A

G

G

Q

D

N

N

I

I

V

V

S

A

T

S

S

S

Y

H

L

L

Y
|
Y

G

G

G

G

T

T

Y

Y

N

N

L

Q

F

L

H

N

Y

V

T

L

L

L

P

P

K

R

L

F

G

G

I

I

T

K

V

T

K

K

F

F

V

V

D

R

T

S

S

G

D

K

P

L

E

E

N

D

V

Y

R

A

R

A

A

A

I

I

D

D

D

D

K

Q

T

T

R

R

L

A

V

I

Y

Y

T

V

E

E

S

S

V

M

G

S

N

N

P

P

K

D

N

Y

N

V

V

V

D

P

D

D

F

F

E

E

A

G

I

I

A

A

R

K

I

I

A

A

H

H

E

E

A

H

G

G

I

I

P

P

F

L

V

V

V

V

D
|
D

N

N

T

T

V

L

T

G

T

A

S

G

-

G

Y

Y

L

Y

F

I

K

R

P

P

L

I

E

E

H

H

G

G

A

A

D

D

I

I

V

V

V

V

Y

H

S
|
S

L

A

T

T

K
|
K

F

W

I

I

G

G

G

G

H

H

G

G

T

T

S

T

I

I

G

G

G

G

A

V

V

I

V

V

D

D

G

S

G

G

K

R

F

F

P

N

W

W

N

N

-

K

-

H

N

S

G

D

R

R

F

F

P

P

E

E

M

M

T

V

E

E

P

P

D

S

P

P

S

S

Y

Y

H

H

G

G

L

L

C

K

Y

Y

W

W

E

E

A

A

L

F

G

G

N

P

L

A

S

T

Y

F

I

I

L

T

K

R

M

I

R

R

I

V

T

E

L

M

L

L

R

R

D

D

M

I

G

G

A

A

C

C

I

L

S

S

P

P

F

F

N

S

S

A

F

Q

L

Q

F

L

L

L

Q

L

G

G

L

I

E

E

T

T

L

L

P

G

V

L

R

R

M

A

A

E

R

R

H

H

V

A

E

Q

N

N

A

T

R

E

A

K

V

L

A

S

Q

K

W

Y

L

F

E

E

K

S

H

S

P

P

L

N

V

V

S

S

W

W

V

V

N

L

Y

W

P

P

G

G

L

S

A

E

S

S

H

H

K

P

D

T

H

Y

G

S

N

Q

A

A

R

K

K

K

Y

Y

L

L

P

T

K

R

G

G

E

F

G

G

A

A

I

M

I

L

G

S

F

I

G

G

I

V

N

K

G

G

G

D

L

A

E

S

A

A

G

G

R

S

R

K

F

V

I

V

D

D

S

G

A

L

K

K

L

L

L

V

S

S

H

N

L

L

A
|
A

N
|
N

I

V

G

G

D

D

A

A

K

K

S

S

L

L

V

A

I

I

H

H

P

P

A

W

S

S

T

T

T
|
T

H

H

Q

E

Q

Q

L

L

S

S

E

E

E

D

E

E

Q

R

L

L

A

A

S

S

G

G

V

V

T

T

Q

E

D

D

Y

M

I

I

R
|
R

L

I

S

S

I

V

G

G

I

I

E

E

E

H

V

V

A

D

D

D

I

I

I

I

A

A

D

D

I

F

D

E

Q

Q

A

S

L

F

A

Q

A

K

S

A

Q

Y

G

G

binding (2S)-2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]but-3-enoic acid: G84 (= G87), Q85 (= Q88), Q88 (≠ I91), Y109 (= Y112), D181 (= D184), S204 (= S206), K207 (= K209), A368 (= A368), N369 (= N369), T384 (= T384), R404 (= R404)

8erjA Crystal structure of fub7 in complex with e-2-aminocrotonate (see paper)
56% identity, 99% coverage: 7:428/428 of query aligns to 4:428/428 of 8erjA

query
sites
8erjA

S

N

F

F

D

E

T

T

L

L

A

Q

L

L

H

H

A

A

G

G

Q

Y

E

T

P

P

D

D

P

P

T

H

T

T

L

R

S

S

R

T

A

A

V

V

P

P

I

I

Y

Y

Q

A

T

T

A

S

S

S

Y

Y

A

T

F

F

K

N

S

D

S

S

E

A

H

H

A

G

A

A

N

R

L

L

F

F

G

G

L

L

K

K

E

E

F

L

G

G

N

N

I

I

Y

Y

T

S

R

R

L

L

M

M

N

N

P

P

T

T

S

V

D

D

V

V

L

F

E

E

K

K

R

R

L

I

A

A

E

A

L

L

D

E

G

G

G

G

V

I

G

A

A

A

L

A

A

A

V

T

A

S

S
|
S

G
|
G

Q
|
Q

A

A

A

A

I
x
Q

T

F

Y

L

A

T

V

I

L

A

N

T

I

L

A

A

Q

K

A

A

G

G

Q

D

N

N

I

I

V

V

S

A

T

S

S

S

Y

H

L

L

Y
|
Y

G

G

G

G

T

T

Y

Y

N

N

L

Q

F

L

H

N

Y

V

T

L

L

L

P

P

K

R

L

F

G

G

I

I

T

K

V

T

K

K

F

F

V

V

D

R

T

S

S

G

D

K

P

L

E

E

N

D

V

Y

R

A

R

A

A

A

I

I

D

D

D

D

K

Q

T

T

R

R

L

A

V

I

Y

Y

T

V

E

E

S

S

V

M

G

S

N
|
N

P

P

K

D

N

Y

N

V

V

V

D

P

D

D

F

F

E

E

A

G

I

I

A

A

R

K

I

I

A

A

H

H

E

E

A

H

G

G

I

I

P

P

F

L

V

V

V

V

D
|
D

N

N

T

T

V

L

T

G

T

A

S

G

-

G

Y

Y

L

Y

F

I

K

R

P

P

L

I

E

E

H

H

G

G

A

A

D

D

I

I

V

V

V

V

Y

H

S
|
S

L

A

T
|
T

K
|
K

F

W

I

I

G

G

G

G

H

H

G

G

T

T

S

T

I

I

G

G

G

G

A

V

V

I

V

V

D

D

G

S

G

G

K

R

F

F

P

N

W

W

N

N

-

K

-

H

N

S

G

D

R

R

F

F

P

P

E

E

M

M

T

V

E

E

P

P

D

S

P

P

S

S

Y

Y

H

H

G

G

L

L

C

K

Y

Y

W

W

E

E

A

A

L

F

G

G

N

P

L

A

S

T

Y

F

I

I

L

T

K

R

M

I

R

R

I

V

T

E

L

M

L

L

R

R

D

D

M

I

G

G

A

A

C

C

I

L

S

S

P

P

F

F

N

S

S

A

F

Q

L

Q

F

L

L

L

Q

L

G

G

L

I

E

E

T

T

L

L

P

G

V

L

R

R

M

A

A

E

R

R

H

H

V

A

E

Q

N

N

A

T

R

E

A

K

V

L

A

S

Q

K

W

Y

L

F

E

E

K

S

H

S

P

P

L

N

V

V

S

S

W

W

V

V

N

L

Y

W

P

P

G

G

L

S

A

E

S

S

H

H

K

P

D

T

H

Y

G

S

N

Q

A

A

R

K

K

K

Y

Y

L

L

P

T

K

R

G

G

E

F

G

G

A

A

I

M

I

L

G

S

F

I

G

G

I

V

N

K

G

G

G

D

L

A

E

S

A

A

G

G

R

S

R

K

F

V

I

V

D

D

S

G

A

L

K

K

L

L

L

V

S

S

H

N

L

L

A

A

N

N

I

V

G

G

D

D

A

A

K

K

S

S

L

L

V

A

I

I

H

H

P

P

A

W

S

S

T

T

T

T

H

H

Q

E

Q

Q

L

L

S

S

E

E

E

D

E

E

Q

R

L

L

A

A

S

S

G

G

V

V

T

T

Q

E

D

D

Y

M

I

I

R
|
R

L

I

S

S

I

V

G

G

I

I

E

E

E

H

V

V

A

D

D

D

I

I

I

I

A

A

D

D

I

F

D

E

Q

Q

A

S

L

F

A

Q

A

K

S

A

Q

Y

G

G

binding (2E)-2-{[(1E)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene]amino}but-2-enoic acid: S83 (= S86), G84 (= G87), Q85 (= Q88), Q88 (≠ I91), Y109 (= Y112), N156 (= N159), D181 (= D184), S204 (= S206), T206 (= T208), K207 (= K209), R404 (= R404)

8wkoA Crystal structure of o-acetylhomoserine sulfhydrylase from lactobacillus plantarum in the closed form (see paper)
56% identity, 97% coverage: 8:424/428 of query aligns to 9:423/425 of 8wkoA

query
sites
8wkoA

F

F

D

E

T

T

L

L

A

Q

L

V

H

H

A

A

G

G

Q

Q

E

T

P

V

D

D

P

E

T

T

T

G

L

-

S

A

R

R

A

A

V

V

P

P

I

I

Y

Y

Q

Q

T

T

A

T

S

S

Y

Y

A

V

F

F

K

K

S

D

S

A

E

K

H

Q

A

A

A

A

N

G

L

R

F

F

G

A

L

L

K

T

E

D

F

A

G

G

N

N

I

I

Y

Y

T

T

R

R

L

L

M

T

N

N

P

P

T

T

S

T

D

D

V

V

L

V

E

D

K

K

R

R

L

V

A

A

E

A

L

L

D

E

G

H

G

G

V

T

G

A

A

G

L

V

A

T

V

L

A

A

S

T

G
|
G

Q
x
S

A

A

A

A

I

I

T

T

Y

A

A

A

V

I

L

L

N

N

I

I

A

A

Q

Q

A

Q

G

G

Q

D

N

E

I

I

V

V

S

A

T

A

S

N

Y

T

L

L

Y
|
Y

G

G

G

G

T

T

Y

Y

N

D

L

L

F

F

H

S

Y

V

T

T

L

L

P

K

K

K

L

L

G

G

I

I

T

T

V

T

K

H

F

F

V

V

D

D

T

P

S

D

D

E

P

P

E

A

N

N

V

F

R

E

R

T

A

A

I

I

D

N

D

D

K

H

T

T

R

K

L

A

V

L

Y

Y

T

V

E
|
E

S

S

V

I

G

G

N

N

P

P

K

G

N

I

N

N

V

L

D

V

D

D

F

F

E

E

A

A

I

I

A

G

R

K

I

I

A

A

H

H

E

D

A

H

G

G

I

I

P

I

F

F

V

I

V

V

D
|
D

N

N

T
|
T

V

F

T

G

T

T

S

P

Y

Y

L

L

F

V

K

R

P

P

L

L

E

E

H

H

G

G

A

A

D

D

I

V

V

V

V

V

Y

H

S
|
S

L
x
A

T
|
T

K

-

F
|
F

I

I

G

G

G
|
G

H
|
H

G

G

T

T

S

T

I
x
M

G

G

G

G

A

V

V

I

V

V

D

E

G

G

G

G

K

Q

F

F

P

D

W

W

N

R

-

A

N

S

G

G

R

K

F

Y

P

P

E

D

M

F

T

T

E

T

P

P

D

D

P

P

S

Q

Y

Y

H

N

G

G

L

L

C

V

Y

F

W

A

E

D

A

-

L

L

G

G

N

G

L

A

S

A

Y

F

I

T

L

T

K

K

M

V

R

R

I

A

T

E

L

T

L

L

R

R

D

D

M

T

G

G

A

A

C

T

I

I

S

S

P

P

F

F

N

N

S

S

F

F

L

L

F

L

L

L

Q
|
Q

G

G

L

L

E

E

T
x
S

L

L

P

S

V

L

R

R

M

V

A

E

R

R

H

H

V

V

E

T

N

N

A

T

R

R

A

K

V

I

A

V

Q

A

W

F

L

L

E

K

K

A

H

H

P

P

L

K

V

V

S

A

W

W

V

I

N

N

Y

Y

P

P

G

E

L

L

A

E

S

D

H

S

K

P

D

Y

H

H

G

D

N

L

A

A

R

T

K

K

Y

Y

L

F

P

P

K

N

G

G

E

V

G

G

A

S

I

I

I

F

G

T

F

L

G

G

I

L

N

T

G

G

G

G

L

E

E

A

A

A

G

G

R

K

R

A

F

L

I

I

D

E

S

H

A

L

K

Q

L

L

L

F

S

S

H

L

L

L

A

A

N

N

I
x
V

G
x
A

D

D

A

A

K

K

S

S

L

L

V

I

I

I

H

H

P

P

A

A

S

S

T

T

T

T

H

H

Q

A

Q

Q

L

L

S

N

E

E

E

Q

E

E

Q

L

L

L

A

A

S

A

G

G

V

V

T

T

Q

P

D

D

Y

L

I

I

R

R

L

I

S

S

I

V

G

G

I

V

E

E

E

N

V

A

A

D

D

D

I

L

I

I

A

A

D

D

I

L

D

D

Q

Q

A

A

L

L

A

A

binding (2S)-2-amino-6-[[3-hydroxy-2-methyl-5-(phosphonooxymethyl)pyridin-4-yl]methylideneamino]hexanoic acid: G87 (= G87), S88 (≠ Q88), Y112 (= Y112), E155 (= E155), D184 (= D184), T186 (= T186), S206 (= S206), A207 (≠ L207), T208 (= T208), F209 (= F210), G212 (= G213), M217 (≠ I218), V369 (≠ I370), A370 (≠ G371)
binding proline: H213 (= H214), Q284 (= Q285), S288 (≠ T289)

8ovhA Crystal structure of o-acetyl-l-homoserine sulfhydrolase from saccharomyces cerevisiae in complex with pyridoxal-5'-phosphate (see paper)
50% identity, 97% coverage: 8:423/428 of query aligns to 5:391/400 of 8ovhA

query
sites
8ovhA

F

F

D

D

T

T

L

V

A

Q

L

L

H

H

A

A

G

G

Q

Q

E

E

P

N

D

-

P

-

T

-

T

-

L

-

S

S

R

R

A

A

V

V

P

P

I

I

Y

Y

Q

A

T

T

A

T

S

S

Y

Y

A

-

F

-

K

-

S

-

S

-

E

-

H

-

A

-

A

-

N

-

L

-

F

-

G

-

L

-

K

-

E

-

F

-

G

-

N

-

I

-

Y

-

T

-

R

-

L

-

M

-

N

N

P

P

T

T

S

S

D

N

V

V

L

L

E

E

K

E

R

R

L

I

A

A

E

A

L

L

D

E

G

G

G

G

V

A

G

A

A

A

L

L

A

A

V

V

A

S

S

S

G
|
G

Q
|
Q

A

A

A

A

I
x
Q

T

T

Y

L

A

A

V

I

L

Q

N

G

I

L

A

A

Q

H

A

T

G

G

Q

D

N

N

I

I

V

V

S

S

T

T

S

S

Y

Y

L

L

Y
|
Y

G

G

G

G

T

T

Y

Y

N

N

L

Q

F

F

H

K

Y

I

T

S

L

F

P

K

K

R

L

F

G

G

I

I

T

E

V

A

K

R

F

F

V

V

D

E

T

G

S

D

D

N

P

P

E

E

N

E

V

F

R

E

R

K

A

V

I

F

D

D

D

E

K

R

T

T

R

K

L

A

V

V

Y

Y

T

L

E

E

S

T

V

I

G

G

N

N

P

P

K

K

N

Y

N

N

V

V

D

P

D

D

F

F

E

E

A

K

I

I

A

V

R

A

I

I

A

A

H

H

E

K

A

H

G

G

I

I

P

P

F

V

V

V

V

V

D
|
D

N

N

T
|
T

V

F

-

G

T

A

T

G

S

G

Y

Y

L

F

F

C

K

Q

P

P

L

I

E

K

H

Y

G

G

A

A

D

D

I

I

V

V

V

T

Y

H

S
|
S

L

A

T
|
T

K
|
K

F

W

I

I

G

G

G

G

H

H

G

G

T

T

S

T

I

I

G

G

G

G

A

I

V

I

V

V

D

D

G

S

G

G

K

K

F

F

P

P

W

W

N

K

N

D

-

Y

-

P

G

E

R

K

F

F

P

P

E

Q

M

F

T

S

E

Q

P

P

D

A

P

E

S

G

Y

Y

H

H

G

G

L

T

C

I

Y

Y

W

N

E

E

A

A

L

Y

G

G

N

N

L

L

S

A

Y

Y

I

I

L

V

K

H

M

V

R

R

I

T

T

E

L

L

L

L

R

R

D

D

M

L

G

G

A

P

C

L

I

M

S

N

P

P

F

F

N

A

S

S

F

F

L

L

F

L

L

L

Q

Q

G

G

L

V

E

E

T

T

L

L

P

S

V

L

R

R

M

A

A

E

R

R

H

H

V

G

E

E

N

N

A

A

R

L

A

K

V

L

A

A

Q

K

W

W

L

L

E

E

K

Q

H

S

P

P

L

Y

V

V

S

S

W

W

V

V

N

S

Y

Y

P

P

G

G

L

L

A

A

S

S

H

H

K

S

D

H

H

H

G

E

N

N

A

A

R

K

K

K

Y

Y

L

L

P

S

K

N

G

G

E

F

G

G

A

G

I

V

I

L

G

S

F

F

G

G

I

V

-

K

-

D

-

L

-

P

N

N

G

A

G

D

L

K

E

E

-

T

-

D

-

P

-

F

-

K

-

L

A

S

G

G

R

A

R

Q

F

V

I

V

D

D

S

N

A

L

K

K

L

L

L

A

S

S

H

N

L

L

A

A

N

N

I

V

G

G

D

D

A

A

K

K

S

T

L

L

V

V

I

I

H

A

P

P

A

Y

S

F

T

T

T

T

H

-

Q

-

Q

-

L

-

S

-

E

-

E

-

E

-

Q

-

L

-

A

-

S

-

G

G

V

V

T

T

Q

K

D

D

Y

L

I

I

R

R

L

V

S

S

I

V

G

G

I

I

E

E

E

F

V

I

A

D

D

D

I

I

I

I

A

A

D

D

I

F

D

Q

Q

Q

A

S

L

F

binding pyridoxal-5'-phosphate: G54 (= G87), Q55 (= Q88), Q58 (≠ I91), Y79 (= Y112), D151 (= D184), T153 (= T186), S174 (= S206), T176 (= T208), K177 (= K209)

4hf8A Crystal structure of l-methionine gamma-lyase from citrobacter freundii with glycine (see paper)
44% identity, 97% coverage: 8:423/428 of query aligns to 8:392/396 of 4hf8A

query
sites
4hf8A

F

F

D

N

T

T

L

Q

A

I

L

V

H

H

A

A

G

G

Q

Q

E

Q

P

P

D

D

P

P

T

S

T

T

L

G

S

A

R

L

A

S

V

T

P

P

I

I

Y

F

Q

Q

T

T

A

S

S

T

Y

F

A

V

F

F

K

D

S

S

S

A

E

E

H

Q

A

G

A

A

N

A

L

R

F

F

G

A

L

L

K

E

E

E

F

S

G

G

N

Y

I

I

Y

Y

T

T

R
|
R

L

L

M

G

N

N

P

P

T

T

S

T

D

D

V

A

L

L

E

E

K

K

R

K

L

L

A

A

E

V

L

L

D

E

G

R

G

G

V

E

G

A

A

G

L

L

A

A

V

T

A

A

S

S

G
|
G

Q
x
I

A

S

A

A

I

I

T

T

Y

T

A

T

V

L

L

L

N

T

I

L

A

C

Q

Q

A

Q

G

G

Q

D

N

H

I

I

V

V

S

S

T

A

S

S

Y

A

L

I

Y
|
Y

G

G

G

C

T

T

Y

H

N

A

L

F

F

L

H

S

Y

H

T

S

L

M

P

P

K

K

L

F

G

G

I

I

T

N

V

V

K

R

F

F

V

V

D

D

T

A

S

A

D

K

P

P

E

E

N

E

V

I

R

R

R

A

A

A

I

M

D

R

D

P

K

E

T

T

R

K

L

V

V

V

Y

Y

T

I

E
|
E

S

T

V

P

G

A

N
|
N

P

P

K

T

N

L

N

S

V

L

D

V

D

D

F

I

E

E

A

T

I

V

A

A

R

G

I

I

A

A

H

H

E

Q

A

Q

G

G

I

A

P

L

F

L

V

V

V

V

D
|
D

N

N

T

T

V

F

T

M

T

S

S

P

Y

Y

L

C

F

Q

K

Q

P

P

L

L

E

Q

H

L

G

G

A

A

D

D

I

I

V

V

V

V

Y

H

S
|
S

L

V

T

T

K
|
K

F

Y

I

I

G

N

G

G

H

H

G

G

T

D

S

V

I

I

G

G

G

G

A

I

V

I

V

V

D

-

G

-

G

-

K

-

F

-

P

-

W

-

N

-

N

-

G

-

R

-

F

-

P

-

E

-

M

-

T

-

E

-

P

-

D

-

P

-

S

-

Y

-

H

-

G

-

L

-

C

-

Y

-

W

-

E

-

A

-

L

-

G

G

N

K

L

Q

S

E

Y

F

I

I

L

D

K

Q

M

A

R

R

I

F

T

V

L

G

L

L

R

K

D

D

M

I

-

T

G

G

A

G

C

C

I

M

S

S

P

P

F

F

N

N

S

A

F

W

L

L

F

T

L

L

Q

R

G

G

L

V

E

K

T

T

L

L

P

G

V

I

R

R

M

M

A

E

R

R

H

H

V

C

E

E

N

N

A

A

R

L

A

K

V

I

A

A

Q

R

W

F

L

L

E

E

K

G

H

H

P

P

L

S

V

I

S

T

W

R

V

V

N

Y

Y

Y

P

P

G

G

L

L

A

S

S

S

H

H

K

P

D

Q

H

Y

-

E

-

L

G

G

N

Q

A

R

R

Q

K

M

Y

S

L

L

P

P

K

-

G

-

E

-

G

G

A

G

I

I

I

I

G

S

F

F

G

E

I

I

N

A

G

G

G

G

L

L

E

E

A

A

G

G

R

R

R

R

F

M

I

I

D

N

S

S

A

V

K

E

L

L

L

C

S

L

H

L

L

A

A

V

N
x
S

I

L

G

G

D

D

A

T

K

E

S

T

L

L

V

I

I

Q

H

H

P

P

A

A

S

S

T

M

T

T

H

H

Q

S

Q

P

L

V

S

A

E

P

E

E

E

E

Q

R

L

L

A

K

S

A

G

G

V

I

T

T

Q

D

D

G

Y

L

I

I

R
|
R

L

L

S

S

I

V

G

G

I

L

E

E

E

D

V

P

A

E

D

D

I

I

I

I

A

N

D

D

I

L

D

E

Q

H

A

A

L

I

active site: R59 (= R59), Y112 (= Y112), D184 (= D184), K209 (= K209)
binding n-glycine-[3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-yl-methane]: G87 (= G87), I88 (≠ Q88), Y112 (= Y112), E155 (= E155), N159 (= N159), D184 (= D184), S206 (= S206), K209 (= K209), S338 (≠ N369), R373 (= R404)

4omaA The crystal structure of methionine gamma-lyase from citrobacter freundii in complex with l-cycloserine pyridoxal-5'-phosphate (see paper)
44% identity, 97% coverage: 8:423/428 of query aligns to 8:392/396 of 4omaA

query
sites
4omaA

F

F

D

N

T

T

L

Q

A

I

L

V

H

H

A

A

G

G

Q

Q

E

Q

P

P

D

D

P

P

T

S

T

T

L

G

S

A

R

L

A

S

V

T

P

P

I

I

Y

F

Q

Q

T

T

A

S

S

T

Y

F

A

V

F

F

K

D

S

S

S

A

E

E

H

Q

A

G

A

A

N

A

L

R

F

F

G

A

L

L

K

E

E

E

F

S

G

G

N

Y

I

I

Y

Y

T

T

R
|
R

L

L

M

G

N

N

P

P

T

T

S

T

D

D

V

A

L

L

E

E

K

K

R

K

L

L

A

A

E

V

L

L

D

E

G

R

G

G

V

E

G

A

A

G

L

L

A

A

V

T

A

A

S

S

G
|
G

Q
x
I

A

S

A

A

I

I

T

T

Y

T

A

T

V

L

L

L

N

T

I

L

A

C

Q

Q

A

Q

G

G

Q

D

N

H

I

I

V

V

S

S

T

A

S

S

Y

A

L

I

Y
|
Y

G

G

G

C

T

T

Y

H

N

A

L

F

F

L

H

S

Y

H

T

S

L

M

P

P

K

K

L

F

G

G

I

I

T

N

V

V

K

S

F

F

V

V

D

D

T

A

S

A

D

K

P

P

E

E

N

E

V

I

R

R

R

A

A

A

I

M

D

R

D

P

K

E

T

T

R

K

L

V

V

V

Y

Y

T

I

E

E

S

T

V

P

G

A

N

N

P

P

K

T

N

L

N

S

V

L

D

V

D

D

F

I

E

E

A

T

I

V

A

A

R

G

I

I

A

A

H

H

E

Q

A

Q

G

G

I

A

P

L

F

L

V

V

V

V

D
|
D

N

N

T

T

V

F

T

M

T

S

S

P

Y

Y

L

C

F

Q

K

Q

P

P

L

L

E

Q

H

L

G

G

A

A

D

D

I

I

V

V

V

V

Y

H

S
|
S

L

V

T
|
T

K
|
K

F

Y

I

I

G

N

G

G

H

H

G

G

T

D

S

V

I

I

G

G

G

G

A

I

V

I

V

V

D

-

G

-

G

-

K

-

F

-

P

-

W

-

N

-

N

-

G

-

R

-

F

-

P

-

E

-

M

-

T

-

E

-

P

-

D

-

P

-

S

-

Y

-

H

-

G

-

L

-

C

-

Y

-

W

-

E

-

A

-

L

-

G

G

N

K

L

Q

S

E

Y

F

I

I

L

D

K

Q

M

A

R

R

I

F

T

V

L

G

L

L

R

K

D

D

M

I

-

T

G

G

A

G

C

C

I

M

S

S

P

P

F

F

N

N

S

A

F

W

L

L

F

T

L

L

Q

R

G

G

L

V

E

K

T

T

L

L

P

G

V

I

R

R

M

M

A

E

R

R

H

H

V

C

E

E

N

N

A

A

R

L

A

K

V

I

A

A

Q

R

W

F

L

L

E

E

K

G

H

H

P

P

L

S

V

I

S

T

W

R

V

V

N

Y

Y

Y

P

P

G

G

L

L

A

S

S

S

H

H

K

P

D

Q

H

Y

-

E

-

L

G

G

N

Q

A

R

R

Q

K

M

Y

S

L

L

P

P

K

-

G

-

E

-

G

G

A

G

I

I

I

I

G

S

F

F

G

E

I

I

N

A

G

G

G

G

L

L

E

E

A

A

G

G

R

R

R

R

F

M

I

I

D

N

S

S

A

V

K

E

L

L

L

C

S

L

H

L

L

A

A
x
V

N
x
S

I

L

G

G

D

D

A

T

K

E

S

T

L

L

V

I

I

Q

H

H

P

P

A

A

S

S

T

M

T

T

H

H

Q

S

Q

P

L

V

S

A

E

P

E

E

E

E

Q

R

L

L

A

K

S

A

G

G

V

I

T

T

Q

D

D

G

Y

L

I

I

R
|
R

L

L

S

S

I

V

G

G

I

L

E

E

E

D

V

P

A

E

D

D

I

I

I

I

A

N

D

D

I

L

D

E

Q

H

A

A

L

I

active site: R59 (= R59), Y112 (= Y112), D184 (= D184), K209 (= K209)
binding [5-hydroxy-6-methyl-4-({[(4E)-3-oxo-1,2-oxazolidin-4-ylidene]amino}methyl)pyridin-3-yl]methyl dihydrogen phosphate: G87 (= G87), I88 (≠ Q88), Y112 (= Y112), D184 (= D184), S206 (= S206), T208 (= T208), K209 (= K209), V337 (≠ A368), S338 (≠ N369), R373 (= R404)

3jwbA Crystal structure of l-methionine gamma-lyase from citrobacter freundii with norleucine (see paper)
44% identity, 97% coverage: 8:423/428 of query aligns to 8:392/396 of 3jwbA

query
sites
3jwbA

F

F

D

N

T

T

L

Q

A

I

L

V

H

H

A

A

G

G

Q

Q

E

Q

P

P

D

D

P

P

T

S

T

T

L

G

S

A

R

L

A

S

V

T

P

P

I

I

Y

F

Q

Q

T

T

A

S

S

T

Y

F

A

V

F

F

K

D

S

S

S

A

E

E

H

Q

A

G

A

A

N

A

L

R

F

F

G

A

L

L

K

E

E

E

F

S

G

G

N

Y

I

I

Y

Y

T

T

R
|
R

L

L

M

G

N

N

P

P

T

T

S

T

D

D

V

A

L

L

E

E

K

K

R

K

L

L

A

A

E

V

L

L

D

E

G

R

G

G

V

E

G

A

A

G

L

L

A

A

V

T

A

A

S

S

G

G

Q

I

A

S

A

A

I

I

T

T

Y

T

A

T

V

L

L

L

N

T

I

L

A

C

Q

Q

A

Q

G

G

Q

D

N

H

I

I

V

V

S

S

T

A

S

S

Y

A

L

I

Y
|
Y

G

G

G

C

T

T

Y

H

N

A

L

F

F

L

H

S

Y

H

T

S

L

M

P

P

K

K

L

F

G

G

I

I

T

N

V

V

K

S

F

F

V

V

D

D

T

A

S

A

D

K

P

P

E

E

N

E

V

I

R

R

R

A

A

A

I

M

D

R

D

P

K

E

T

T

R

K

L

V

V

V

Y

Y

T

I

E

E

S

T

V

P

G

A

N

N

P

P

K

T

N

L

N

S

V

L

D

V

D

D

F

I

E

E

A

T

I

V

A

A

R

G

I

I

A

A

H

H

E

Q

A

Q

G

G

I

A

P

L

F

L

V

V

V

V

D
|
D

N

N

T

T

V

F

T

M

T

S

S

P

Y

Y

L

C

F

Q

K

Q

P

P

L

L

E

Q

H

L

G

G

A

A

D

D

I

I

V

V

V

V

Y

H

S

S

L

V

T

T

K
|
K

F

Y

I

I

G

N

G

G

H

H

G

G

T

D

S

V

I

I

G

G

G

G

A

I

V

I

V

V

D

-

G

-

G

-

K

-

F

-

P

-

W

-

N

-

N

-

G

-

R

-

F

-

P

-

E

-

M

-

T

-

E

-

P

-

D

-

P

-

S

-

Y

-

H

-

G

-

L

-

C

-

Y

-

W

-

E

-

A

-

L

-

G

G

N

K

L

Q

S

E

Y

F

I

I

L

D

K

Q

M

A

R

R

I

F

T

V

L

G

L

L

R

K

D

D

M

I

-

T

G

G

A

G

C

C

I

M

S

S

P

P

F

F

N

N

S

A

F

W

L

L

F

T

L

L

Q

R

G

G

L

V

E

K

T

T

L

L

P

G

V

I

R

R

M

M

A

E

R

R

H

H

V

C

E

E

N

N

A

A

R

L

A

K

V

I

A

A

Q

R

W

F

L

L

E

E

K

G

H

H

P

P

L

S

V

I

S

T

W

R

V

V

N

Y

Y

Y

P

P

G

G

L

L

A

S

S

S

H

H

K

P

D

Q

H

Y

-

E

-

L

G

G

N

Q

A

R

R

Q

K

M

Y

S

L

L

P

P

K

-

G

-

E

-

G

G

A

G

I

I

I

I

G

S

F

F

G

E

I

I

N

A

G

G

G

G

L

L

E

E

A

A

G

G

R

R

R

R

F

M

I

I

D

N

S

S

A

V

K

E

L

L

L

C

S

L

H

L

L

A

A

V

N
x
S

I

L

G

G

D

D

A

T

K

E

S

T

L

L

V

I

I

Q

H

H

P

P

A

A

S

S

T

M

T

T

H

H

Q

S

Q

P

L

V

S

A

E

P

E

E

E

E

Q

R

L

L

A

K

S

A

G

G

V

I

T

T

Q

D

D

G

Y

L

I

I

R
|
R

L

L

S

S

I

V

G

G

I

L

E

E

E

D

V

P

A

E

D

D

I

I

I

I

A

N

D

D

I

L

D

E

Q

H

A

A

L

I

active site: R59 (= R59), Y112 (= Y112), D184 (= D184), K209 (= K209)
binding norleucine: Y112 (= Y112), K209 (= K209), S338 (≠ N369), R373 (= R404)

3jwaA Crystal structure of l-methionine gamma-lyase from citrobacter freundii with methionine phosphinate (see paper)
44% identity, 97% coverage: 8:423/428 of query aligns to 8:392/396 of 3jwaA

query
sites
3jwaA

F

F

D

N

T

T

L

Q

A

I

L

V

H

H

A

A

G

G

Q

Q

E

Q

P

P

D

D

P

P

T

S

T

T

L

G

S

A

R

L

A

S

V

T

P

P

I

I

Y

F

Q

Q

T

T

A

S

S

T

Y

F

A

V

F

F

K

D

S

S

S

A

E

E

H

Q

A

G

A

A

N

A

L

R

F

F

G

A

L

L

K

E

E

E

F

S

G

G

N

Y

I

I

Y

Y

T

T

R
|
R

L

L

M

G

N

N

P

P

T

T

S

T

D

D

V

A

L

L

E

E

K

K

R

K

L

L

A

A

E

V

L

L

D

E

G

R

G

G

V

E

G

A

A

G

L

L

A

A

V

T

A

A

S

S

G

G

Q

I

A

S

A

A

I

I

T

T

Y

T

A

T

V

L

L

L

N

T

I

L

A

C

Q

Q

A

Q

G

G

Q

D

N

H

I

I

V

V

S

S

T

A

S

S

Y

A

L

I

Y
|
Y

G

G

G

C

T

T

Y

H

N

A

L

F

F

L

H

S

Y

H

T

S

L

M

P

P

K

K

L

F

G

G

I

I

T

N

V

V

K

S

F

F

V

V

D

D

T

A

S

A

D

K

P

P

E

E

N

E

V

I

R

R

R

A

A

A

I

M

D

R

D

P

K

E

T

T

R

K

L

V

V

V

Y

Y

T

I

E

E

S

T

V

P

G

A

N

N

P

P

K

T

N

L

N

S

V

L

D

V

D

D

F

I

E

E

A

T

I

V

A

A

R

G

I

I

A

A

H

H

E

Q

A

Q

G

G

I

A

P

L

F

L

V

V

V

V

D
|
D

N

N

T

T

V

F

T

M

T

S

S

P

Y

Y

L

C

F

Q

K

Q

P

P

L

L

E

Q

H

L

G

G

A

A

D

D

I

I

V

V

V

V

Y

H

S

S

L

V

T

T

K
|
K

F

Y

I

I

G

N

G

G

H

H

G

G

T

D

S

V

I

I

G

G

G

G

A

I

V

I

V

V

D

-

G

-

G

-

K

-

F

-

P

-

W

-

N

-

N

-

G

-

R

-

F

-

P

-

E

-

M

-

T

-

E

-

P

-

D

-

P

-

S

-

Y

-

H

-

G

-

L

-

C

-

Y

-

W

-

E

-

A

-

L

-

G

G

N

K

L

Q

S

E

Y

F

I

I

L

D

K

Q

M

A

R

R

I

F

T

V

L

G

L

L

R

K

D

D

M

I

-

T

G

G

A

G

C

C

I

M

S

S

P

P

F

F

N

N

S

A

F

W

L

L

F

T

L

L

Q

R

G

G

L

V

E

K

T

T

L

L

P

G

V

I

R

R

M

M

A

E

R

R

H

H

V

C

E

E

N

N

A

A

R

L

A

K

V

I

A

A

Q

R

W

F

L

L

E

E

K

G

H

H

P

P

L

S

V

I

S

T

W

R

V

V

N

Y

Y

Y

P

P

G

G

L

L

A

S

S

S

H

H

K

P

D

Q

H

Y

-

E

-

L

G

G

N

Q

A

R

R

Q

K

M

Y

S

L

L

P

P

K

-

G

-

E

-

G

G

A

G

I

I

I

I

G

S

F

F

G

E

I

I

N

A

G

G

G

G

L

L

E

E

A

A

G

G

R

R

R

R

F

M

I

I

D

N

S

S

A

V

K

E

L

L

L

C

S

L

H

L

L

A

A
x
V

N
x
S

I

L

G

G

D

D

A

T

K

E

S

T

L

L

V

I

I

Q

H

H

P

P

A

A

S

S

T

M

T

T

H

H

Q

S

Q

P

L

V

S

A

E

P

E

E

E

E

Q

R

L

L

A

K

S

A

G

G

V

I

T

T

Q

D

D

G

Y

L

I

I

R
|
R

L

L

S

S

I

V

G

G

I

L

E

E

E

D

V

P

A

E

D

D

I

I

I

I

A

N

D

D

I

L

D

E

Q

H

A

A

L

I

active site: R59 (= R59), Y112 (= Y112), D184 (= D184), K209 (= K209)
binding (1-amino-3-methylsulfanyl-propyl)-phosphinic acid: Y112 (= Y112), K209 (= K209), V337 (≠ A368), S338 (≠ N369), R373 (= R404)

3jw9A Crystal structure of l-methionine gamma-lyase from citrobacter freundii with s-ethyl-cysteine (see paper)
44% identity, 97% coverage: 8:423/428 of query aligns to 8:392/396 of 3jw9A

query
sites
3jw9A

F

F

D

N

T

T

L

Q

A

I

L

V

H

H

A

A

G

G

Q

Q

E

Q

P

P

D

D

P

P

T

S

T

T

L

G

S

A

R

L

A

S

V

T

P

P

I

I

Y

F

Q

Q

T

T

A

S

S

T

Y

F

A

V

F

F

K

D

S

S

S

A

E

E

H

Q

A

G

A

A

N

A

L

R

F

F

G

A

L

L

K

E

E

E

F

S

G

G

N

Y

I

I

Y

Y

T

T

R
|
R

L

L

M

G

N

N

P

P

T

T

S

T

D

D

V

A

L

L

E

E

K

K

R

K

L

L

A

A

E

V

L

L

D

E

G

R

G

G

V

E

G

A

A

G

L

L

A

A

V

T

A

A

S

S

G

G

Q

I

A

S

A

A

I

I

T

T

Y

T

A

T

V

L

L

L

N

T

I

L

A

C

Q

Q

A

Q

G

G

Q

D

N

H

I

I

V

V

S

S

T

A

S

S

Y

A

L

I

Y
|
Y

G

G

G

C

T

T

Y

H

N

A

L

F

F

L

H

S

Y

H

T

S

L

M

P

P

K

K

L

F

G

G

I

I

T

N

V

V

K

S

F

F

V

V

D

D

T

A

S

A

D

K

P

P

E

E

N

E

V

I

R

R

R

A

A

A

I

M

D

R

D

P

K

E

T

T

R

K

L

V

V

V

Y

Y

T

I

E

E

S

T

V

P

G

A

N

N

P

P

K

T

N

L

N

S

V

L

D

V

D

D

F

I

E

E

A

T

I

V

A

A

R

G

I

I

A

A

H

H

E

Q

A

Q

G

G

I

A

P

L

F

L

V

V

V

V

D
|
D

N

N

T

T

V

F

T

M

T

S

S

P

Y

Y

L

C

F

Q

K

Q

P

P

L

L

E

Q

H

L

G

G

A

A

D

D

I

I

V

V

V

V

Y

H

S

S

L

V

T

T

K
|
K

F

Y

I

I

G

N

G

G

H

H

G

G

T

D

S

V

I

I

G

G

G

G

A

I

V

I

V

V

D

-

G

-

G

-

K

-

F

-

P

-

W

-

N

-

N

-

G

-

R

-

F

-

P

-

E

-

M

-

T

-

E

-

P

-

D

-

P

-

S

-

Y

-

H

-

G

-

L

-

C

-

Y

-

W

-

E

-

A

-

L

-

G

G

N

K

L

Q

S

E

Y

F

I

I

L

D

K

Q

M

A

R

R

I

F

T

V

L

G

L

L

R

K

D

D

M

I

-

T

G

G

A

G

C

C

I

M

S

S

P

P

F

F

N

N

S

A

F

W

L

L

F

T

L

L

Q

R

G

G

L

V

E

K

T

T

L

L

P

G

V

I

R

R

M

M

A

E

R

R

H

H

V

C

E

E

N

N

A

A

R

L

A

K

V

I

A

A

Q

R

W

F

L

L

E

E

K

G

H

H

P

P

L

S

V

I

S

T

W

R

V

V

N

Y

Y

Y

P

P

G

G

L

L

A

S

S

S

H

H

K

P

D

Q

H

Y

-

E

-

L

G

G

N

Q

A

R

R

Q

K

M

Y

S

L

L

P

P

K

-

G

-

E

-

G

G

A

G

I

I

I

I

G

S

F

F

G

E

I

I

N

A

G

G

G

G

L

L

E

E

A

A

G

G

R

R

R

R

F

M

I

I

D

N

S

S

A

V

K

E

L

L

L

C

S

L

H

L

L

A

A
x
V

N
x
S

I

L

G

G

D

D

A

T

K

E

S

T

L

L

V

I

I

Q

H

H

P

P

A

A

S

S

T

M

T

T

H

H

Q

S

Q

P

L

V

S

A

E

P

E

E

E

E

Q

R

L

L

A

K

S

A

G

G

V

I

T

T

Q

D

D

G

Y

L

I

I

R

R

L

L

S

S

I

V

G

G

I

L

E

E

E

D

V

P

A

E

D

D

I

I

I

I

A

N

D

D

I

L

D

E

Q

H

A

A

L

I

active site: R59 (= R59), Y112 (= Y112), D184 (= D184), K209 (= K209)
binding S-ethyl-L-cysteine: Y112 (= Y112), K209 (= K209), V337 (≠ A368), S338 (≠ N369)

5m3zA Crystal structure of citrobacter freundii methionine gamma-lyase with c115h replacement in the complex with l-norleucine (see paper)
44% identity, 97% coverage: 8:423/428 of query aligns to 7:391/395 of 5m3zA

query
sites
5m3zA

F

F

D

N

T

T

L

Q

A

I

L

V

H

H

A

A

G

G

Q

Q

E

Q

P

P

D

D

P

P

T

S

T

T

L

G

S

A

R

L

A

S

V

T

P

P

I

I

Y

F

Q

Q

T

T

A

S

S

T

Y

F

A

V

F

F

K

D

S

S

S

A

E

E

H

Q

A

G

A

A

N

A

L

R

F

F

G

A

L

L

K

E

E

E

F

S

G

G

N

Y

I

I

Y

Y

T

T

R
|
R

L

L

M

G

N

N

P

P

T

T

S

T

D

D

V

A

L

L

E

E

K

K

R

K

L

L

A

A

E

V

L

L

D

E

G

R

G

G

V

E

G

A

A

G

L

L

A

A

V

T

A

A

S

S

G
|
G

Q
x
I

A

S

A

A

I

I

T

T

Y

T

A

T

V

L

L

L

N

T

I

L

A

C

Q

Q

A

Q

G

G

Q

D

N

H

I

I

V

V

S

S

T

A

S

S

Y

A

L

I

Y
|
Y

G

G

G
x
H

T

T

Y

H

N

A

L

F

F

L

H

S

Y

H

T

S

L

M

P

P

K

K

L

F

G

G

I

I

T

N

V

V

K

S

F

F

V

V

D

D

T

A

S

A

D

K

P

P

E

E

N

E

V

I

R

R

R

A

A

A

I

M

D

R

D

P

K

E

T

T

R

K

L

V

V

V

Y

Y

T

I

E
|
E

S

T

V

P

G

A

N

N

P

P

K

T

N

L

N

S

V

L

D

V

D

D

F

I

E

E

A

T

I

V

A

A

R

G

I

I

A

A

H

H

E

Q

A

Q

G

G

I

A

P

L

F

L

V

V

V

V

D
|
D

N

N

T
|
T

V

F

T

M

T

S

S

P

Y

Y

L

C

F

Q

K

Q

P

P

L

L

E

Q

H

L

G

G

A

A

D

D

I

I

V

V

V

V

Y

H

S
|
S

L

V

T
|
T

K
|
K

F

Y

I

I

G

N

G

G

H

H

G

G

T

D

S

V

I

I

G

G

G

G

A

I

V

I

V

V

D

-

G

-

G

-

K

-

F

-

P

-

W

-

N

-

N

-

G

-

R

-

F

-

P

-

E

-

M

-

T

-

E

-

P

-

D

-

P

-

S

-

Y

-

H

-

G

-

L

-

C

-

Y

-

W

-

E

-

A

-

L

-

G

G

N

K

L

Q

S

E

Y

F

I

I

L

D

K

Q

M

A

R

R

I

F

T

V

L

G

L

L

R

K

D

D

M

I

-

T

G

G

A

G

C

C

I

M

S

S

P

P

F

F

N

N

S

A

F

W

L

L

F

T

L

L

Q

R

G

G

L

V

E

K

T

T

L

L

P

G

V

I

R

R

M

M

A

E

R

R

H

H

V

C

E

E

N

N

A

A

R

L

A

K

V

I

A

A

Q

R

W

F

L

L

E

E

K

G

H

H

P

P

L

S

V

I

S

T

W

R

V

V

N

Y

Y

Y

P

P

G

G

L

L

A

S

S

S

H

H

K

P

D

Q

H

Y

-

E

-

L

G

G

N

Q

A

R

R

Q

K

M

Y

S

L

L

P

P

K

-

G

-

E

-

G

G

A

G

I

I

I

I

G

S

F

F

G

E

I

I

N

A

G

G

G

G

L

L

E

E

A

A

G

G

R

R

R

R

F

M

I

I

D

N

S

S

A

V

K

E

L

L

L

C

S

L

H

L

L

A

A
x
V

N
x
S

I

L

G

G

D

D

A

T

K

E

S

T

L

L

V

I

I

Q

H

H

P

P

A

A

S

S

T

M

T

T

H

H

Q

S

Q

P

L

V

S

A

E

P

E

E

E

E

Q

R

L

L

A

K

S

A

G

G

V

I

T

T

Q

D

D

G

Y

L

I

I

R
|
R

L

L

S

S

I

V

G

G

I

L

E

E

E

D

V

P

A

E

D

D

I

I

I

I

A

N

D

D

I

L

D

E

Q

H

A

A

L

I

active site: R58 (= R59), Y111 (= Y112), D183 (= D184), K208 (= K209)
binding norleucine: Y111 (= Y112), H113 (≠ G114), K208 (= K209), V336 (≠ A368), S337 (≠ N369)
binding pyridoxal-5'-phosphate: G86 (= G87), I87 (≠ Q88), Y111 (= Y112), E154 (= E155), D183 (= D184), T185 (= T186), S205 (= S206), T207 (= T208), K208 (= K209)
binding 2-[o-phosphonopyridoxyl]-amino-hexanoic acid: G86 (= G87), I87 (≠ Q88), Y111 (= Y112), D183 (= D184), S205 (= S206), T207 (= T208), K208 (= K209), V336 (≠ A368), S337 (≠ N369), R372 (= R404)

6egrA Crystal structure of citrobacter freundii methionine gamma-lyase with v358y replacement (see paper)
44% identity, 97% coverage: 8:423/428 of query aligns to 8:392/396 of 6egrA

query
sites
6egrA

F

F

D

N

T

T

L

Q

A

I

L

V

H

H

A

A

G

G

Q

Q

E

Q

P

P

D

D

P

P

T

S

T

T

L

G

S

A

R

L

A

S

V

T

P

P

I

I

Y

F

Q

Q

T

T

A

S

S

T

Y

F

A

V

F

F

K

D

S

S

S

A

E

E

H

Q

A

G

A

A

N

A

L

R

F

F

G

A

L

L

K

E

E

E

F

S

G

G

N

Y

I

I

Y

Y

T

T

R

R

L

L

M

G

N

N

P

P

T

T

S

T

D

D

V

A

L

L

E

E

K

K

R

K

L

L

A

A

E

V

L

L

D

E

G

R

G

G

V

E

G

A

A

G

L

L

A

A

V

T

A

A

S

S

G
|
G

Q
x
I

A

S

A

A

I

I

T

T

Y

T

A

T

V

L

L

L

N

T

I

L

A

C

Q

Q

A

Q

G

G

Q

D

N

H

I

I

V

V

S

S

T

A

S

S

Y

A

L

I

Y
|
Y

G

G

G

C

T

T

Y

H

N

A

L

F

F

L

H

S

Y

H

T

S

L

M

P

P

K

K

L

F

G

G

I

I

T

N

V

V

K

S

F

F

V

V

D

D

T

A

S

A

D

K

P

P

E

E

N

E

V

I

R

R

R

A

A

A

I

M

D

R

D

P

K

E

T

T

R

K

L

V

V

V

Y

Y

T

I

E
|
E

S

T

V

P

G

A

N

N

P

P

K

T

N

L

N

S

V

L

D

V

D

D

F

I

E

E

A

T

I

V

A

A

R

G

I

I

A

A

H

H

E

Q

A

Q

G

G

I

A

P

L

F

L

V

V

V

V

D
|
D

N

N

T
|
T

V
x
F

T

M

T

S

S

P

Y

Y

L

C

F

Q

K

Q

P

P

L

L

E

Q

H

L

G

G

A

A

D

D

I

I

V

V

V

V

Y

H

S
|
S

L

V

T
|
T

K
|
K

F

Y

I

I

G

N

G

G

H

H

G

G

T

D

S

V

I

I

G

G

G

G

A

I

V

I

V

V

D

-

G

-

G

-

K

-

F

-

P

-

W

-

N

-

N

-

G

-

R

-

F

-

P

-

E

-

M

-

T

-

E

-

P

-

D

-

P

-

S

-

Y

-

H

-

G

-

L

-

C

-

Y

-

W

-

E

-

A

-

L

-

G

G

N

K

L

Q

S

E

Y

F

I

I

L

D

K

Q

M

A

R

R

I

F

T

V

L

G

L

L

R

K

D

D

M

I

-

T

G

G

A

G

C

C

I

M

S

S

P

P

F

F

N

N

S

A

F

W

L

L

F

T

L

L

Q

R

G

G

L

V

E

K

T

T

L

L

P

G

V

I

R

R

M

M

A

E

R

R

H

H

V

C

E

E

N

N

A

A

R

L

A

K

V

I

A

A

Q

R

W

F

L

L

E

E

K

G

H

H

P

P

L

S

V

I

S

T

W

R

V

V

N

Y

Y

Y

P

P

G

G

L

L

A

S

S

S

H

H

K

P

D

Q

H

Y

-

E

-

L

G

G

N

Q

A

R

R

Q

K

M

Y

S

L

L

P

P

K

-

G

-

E

-

G

G

A

G

I

I

I

I

G

S

F

F

G

E

I

I

N

A

G

G

G

G

L

L

E

E

A

A

G

G

R

R

R

R

F

M

I

I

D

N

S

S

A

V

K

E

L

L

L

C

S

L

H

L

L

A

A

V

N

S

I

L

G

G

D

D

A

T

K

E

S

T

L

L

V

I

I

Q

H

H

P

P

A

A

S

S

T

M

T

T

H

H

Q

S

Q

P

L

Y

S

A

E

P

E

E

E

E

Q

R

L

L

A

K

S

A

G

G

V

I

T

T

Q

D

D

G

Y

L

I

I

R

R

L

L

S

S

I

V

G

G

I

L

E

E

E

D

V

P

A

E

D

D

I

I

I

I

A

N

D

D

I

L

D

E

Q

H

A

A

L

I

binding pyridoxal-5'-phosphate: G87 (= G87), I88 (≠ Q88), Y112 (= Y112), E155 (= E155), D184 (= D184), T186 (= T186), F187 (≠ V187), S206 (= S206), T208 (= T208), K209 (= K209)

3mkjA Methionine gamma-lyase from citrobacter freundii with pyridoximine-5'- phosphate (see paper)
42% identity, 97% coverage: 8:423/428 of query aligns to 8:381/386 of 3mkjA

query
sites
3mkjA

F

F

D

N

T

T

L

Q

A

I

L

V

H

H

A

A

G

G

Q

Q

E

Q

P

P

D

D

P

P

T

S

T

T

L

G

S

A

R

L

A

S

V

T

P

P

I

I

Y

F

Q

Q

T

T

A

S

S

T

Y

F

A

V

F

F

K

D

S

S

S

A

E

E

H

Q

A

G

A

A

N

A

L

R

F

F

G

-

L

-

K

-

E

-

F

-

G

-

N

-

I

-

Y

-

T

-

R

-

L

L

M

G

N

N

P

P

T

T

S

T

D

D

V

A

L

L

E

E

K

K

R

K

L

L

A

A

E

V

L

L

D

E

G

R

G

G

V

E

G

A

A

G

L

L

A

A

V

T

A

A

S

S

G
|
G

Q
x
I

A

S

A

A

I

I

T

T

Y

T

A

T

V

L

L

L

N

T

I

L

A

C

Q

Q

A

Q

G

G

Q

D

N

H

I

I

V

V

S

S

T

A

S

S

Y

A

L

I

Y
|
Y

G

G

G

C

T

T

Y

H

N

A

L

F

F

L

H

S

Y

H

T

S

L

M

P

P

K

K

L

F

G

G

I

I

T

N

V

V

K

S

F

F

V

V

D

D

T

A

S

A

D

K

P

P

E

E

N

E

V

I

R

R

R

A

A

A

I

M

D

R

D

P

K

E

T

T

R

K

L

V

V

V

Y

Y

T

I

E
|
E

S

T

V

P

G

A

N

N

P

P

K

T

N

L

N

S

V

L

D

V

D

D

F

I

E

E

A

T

I

V

A

A

R

G

I

I

A

A

H

H

E

Q

A

Q

G

G

I

A

P

L

F

L

V

V

V

V

D
|
D

N

N

T

T

V
x
F

T

M

T

S

S

P

Y

Y

L

C

F

Q

K

Q

P

P

L

L

E

Q

H

L

G

G

A

A

D

D

I

I

V

V

V

V

Y

H

S
|
S

L

V

T
|
T

K
|
K

F

Y

I

I

G

N

G

G

H

H

G

G

T

D

S

V

I

I

G

G

G

G

A

I

V

I

V

V

D

-

G

-

G

-

K

-

F

-

P

-

W

-

N

-

N

-

G

-

R

-

F

-

P

-

E

-

M

-

T

-

E

-

P

-

D

-

P

-

S

-

Y

-

H

-

G

-

L

-

C

-

Y

-

W

-

E

-

A

-

L

-

G

G

N

K

L

Q

S

E

Y

F

I

I

L

D

K

Q

M

A

R

R

I

F

T

V

L

G

L

L

R

K

D

D

M

I

-

T

G

G

A

G

C

C

I

M

S

S

P

P

F

F

N

N

S

A

F

W

L

L

F

T

L

L

Q

R

G

G

L

V

E

K

T

T

L

L

P

G

V

I

R

R

M

M

A

E

R

R

H

H

V

C

E

E

N

N

A

A

R

L

A

K

V

I

A

A

Q

R

W

F

L

L

E

E

K

G

H

H

P

P

L

S

V

I

S

T

W

R

V

V

N

Y

Y

Y

P

P

G

G

L

L

A

S

S

S

H

H

K

P

D

Q

H

Y

-

E

-

L

G

G

N

Q

A

R

R

Q

K

M

Y

S

L

L

P

P

K

-

G

-

E

-

G

G

A

G

I

I

I

I

G

S

F

F

G

E

I

I

N

A

G

G

G

G

L

L

E

E

A

A

G

G

R

R

R

R

F

M

I

I

D

N

S

S

A

V

K

E

L

L

L

C

S

L

H

L

L

A

A

V

N

S

I

L

G

G

D

D

A

T

K

E

S

T

L

L

V

I

I

Q

H

H

P

P

A

A

S

S

T

M

T

T

H

H

Q

S

Q

P

L

V

S

A

E

P

E

E

E

E

Q

R

L

L

A

K

S

A

G

G

V

I

T

T

Q

D

D

G

Y

L

I

I

R

R

L

L

S

S

I

V

G

G

I

L

E

E

E

D

V

P

A

E

D

D

I

I

I

I

A

N

D

D

I

L

D

E

Q

H

A

A

L

I

active site: Y101 (= Y112), D173 (= D184), K198 (= K209)
binding [5-hydroxy-4-(iminomethyl)-6-methyl-pyridin-3-yl]methyl dihydrogen phosphate: G76 (= G87), I77 (≠ Q88), Y101 (= Y112), E144 (= E155), D173 (= D184), F176 (≠ V187), S195 (= S206), T197 (= T208), K198 (= K209)

Q5SJ58 O-acetyl-L-homoserine sulfhydrylase 2; OAH-sulfhydrylase 2; EC 2.5.1.- from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)
42% identity, 98% coverage: 6:425/428 of query aligns to 1:411/412 of Q5SJ58

query
sites
Q5SJ58

I

M

S

E

F

Y

D

T

T

T

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L

A

A

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A

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-

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R

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A

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L

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P

I

I

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Y

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A

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V

A

A

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A

Y

Y

A

G

F

F

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K

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S

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E

E

H

E

A

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A

Q

N

E

L

R

F

F

G

A

L

T

K

G

E

E

F

-

G

G

N

Y

I

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Y

Y

T

A

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R

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N

D

P

P

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T

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A

D

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V

A

L

L

E

E

K

E

R

R

L

L

A

K

E

A

L

L

D

E

G

G

G

A

V

L

G

E

A

A

L

V

A

V

V

L

A

A

S

S

G

G

Q

Q

A

A

A

A

I

T

T

F

Y

A

A

A

V

L

L

L

N

A

I

L

A

L

Q

R

A

P

G

G

Q

D

N

E

I

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V

V

S

A

T

A

S

K

Y

G

L

L

Y

F

G

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G

Q

T

T

Y

I

N

G

L

L

F

F

H

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Y

Q

T

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L

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S

K

L

L

M

G

G

I

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T

T

V

V

K

R

F

Y

V

V

D

D

T

-

S

P

D

E

P

P

E

E

N

A

V

V

R

R

R

E

A

A

I

L

D

S

D

A

K

K

T

T

R

R

L

A

V

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Y

F

T

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E

E

S

T

V

V

G

A

N

N

P

P

K

A

N

L

N

L

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V

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P

D

D

F

L

E

E

A

A

I

L

A

A

R

T

I

L

A

A

H

E

E

E

A

A

G

G

I

V

P

A

F

L

V

V

V

V

D

D

N

N

T

T

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F

-

G

T

A

T

A

S

G

Y

A

L

L

F

C

K

R

P

P

L

L

E

A

H

W

G

G

A

A

D

H

I

V

V

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V

V

Y

E

S

S

L

L

T

T

K
|
K

F

W

I

A

G

S

G

G

H

H

G

G

T

S

S

V

I

L

G

G

G

G

A

A

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D

S

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G

E

K

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E

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N

R

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N

G

-

R

-

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-

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A

A

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N

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F

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L

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D

L

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C

G

G

A

M

C

A

I

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S

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P

F

F

N

N

S

A

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Y

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Q

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A

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E

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T

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R

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L

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Q

K

G

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W

A

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N

R

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Y

P

P

G

G

L

L

A

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S

E

H

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K

P

D

A

H

H

G

R

N

N

A

A

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K

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Y

L

L

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A

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S

G

G

E

-

G

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A

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I

I

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F

L

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D

I

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N

-

G

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L

L

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A

R

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A

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R

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F

I

L

D

G

S

A

A

I

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R

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L

L

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H

A

L

-

A

A

N

N

I

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D

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L

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L

L

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K

E

E

E

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E

A

Q

R

L

L

A

Q

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A

G

G

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V

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T

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P

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G

Y

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R

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G

I

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A

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E

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A

K202 (= K209) modified: N6-(pyridoxal phosphate)lysine

2cb1A Crystal structure of o-actetyl homoserine sulfhydrylase from thermus thermophilus hb8,oah2.
42% identity, 98% coverage: 6:425/428 of query aligns to 1:404/404 of 2cb1A

query
sites
2cb1A

I

M

S

E

F

Y

D

T

T

T

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L

A

A

L

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H

L

A

A

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L

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P

E

D

D

P

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H

T

G

L

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S

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A

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P

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I

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Y

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A

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V

A

A

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A

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Y

A

G

F

F

K

K

S

T

S

L

E

E

H

E

A

G

A

Q

N

E

L

R

F

F

G

A

L

T

K

G

E

E

F

-

G

G

N

Y

I

V

Y

Y

T

A

R
|
R

L

Q

M

K

N

D

P

P

T

T

S

A

D

K

V

A

L

L

E

E

K

E

R

R

L

L

A

K

E

A

L

L

D

E

G

G

G

A

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L

G

E

A

A

L

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A

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A

A

S
|
S

G
|
G

Q
|
Q

A

A

A

A

I

T

T

F

Y

A

A

A

V

L

L

L

N

A

I

L

A

L

Q

R

A

P

G

G

Q

D

N

E

I

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V

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S

A

T

A

S

K

Y

G

L

L

Y
x
F

G

G

G

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T

T

Y

I

N

G

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L

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F

H

G

Y

Q

T

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L

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S

K

L

L

M

G

G

I

V

T

T

V

V

K

R

F

Y

V

V

D

D

T

-

S

P

D

E

P

P

E

E

N

A

V

V

R

R

R

E

A

A

I

L

D

S

D

A

K

K

T

T

R

R

L

A

V

V

Y

F

T

V

E

E

S

T

V

V

G

A

N

N

P

P

K

A

N

L

N

L

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D

P

D

D

F

L

E

E

A

A

I

L

A

A

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T

I

L

A

A

H

E

E

E

A

A

G

G

I

V

P

A

F

L

V

V

V

V

D
|
D

N

N

T
|
T

V

F

-

G

T

A

T

A

S

G

Y

A

L

L

F

C

K

R

P

P

L

L

E

A

H

W

G

G

A

A

D

H

I

V

V

V

V

V

Y

E

S
|
S

L

L

T
|
T

K
|
K

F

W

I

A

G

S

G

G

H

H

G

G

T

S

S

V

I

L

G

G

G

G

A

A

V

V

V

L

D

S

G

R

G

E

K

T

F

E

P

L

W

W

N

R

N

N

G

-

R

-

F

Y

P

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Q

M

F

T

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E

Q

P

P

D

-

P

-

S

-

Y

-

H

-

G

-

L

-

C

-

Y

-

W

W

E

E

A

A

L

L

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N

A

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C

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F

I

P

L

E

K

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I

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T

L

L

G

L

L

R

S

D

L

M

C

G

G

A

M

C

A

I

L

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S

P

P

F

F

N

N

S

A

F

Y

L

L

F

L

L

F

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Q

G

G

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L

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E

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T

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A

V

L

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R

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A

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E

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T

A

A

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A

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A

A

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W

R

L

L

E

Q

K

G

H

H

P

P

L

K

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V

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K

W

A

V

L

N

R

Y

Y

P

P

G

G

L

L

A

P

S

E

H

D

K

P

D

A

H

H

G

R

N

N

A

A

R

R

K

K

Y

Y

L

L

P

A

K

S

G

G

E

-

G

G

A

P

I

I

I

L

G

T

F

L

G

D

I

L

N

-

G

G

G

D

L

L

E

E

A

R

G

A

R

S

R

R

F

F

I

L

D

G

S

A

A

I

K

R

L

L

L

L

S

K

H

A

L

-

A

A

N

N

I

L

G

G

D

D

A

A

K

R

S

T

L

L

V

L

I

V

H

H

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P

A

W

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T

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H

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S

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R

L

L

S

K

E

E

E

E

E

A

Q

R

L

L

A

Q

S

A

G

G

V

V

T

T

Q

P

D

G

Y

L

I

V

R

R

L

V

S

S

I

V

G

G

I

L

E

E

E

D

V

P

A

L

D

D

I

L

I

L

A

A

D

L

I

F

D

E

Q

E

A

A

L

L

A

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A
|
A

active site: R52 (= R59), F105 (≠ Y112), D176 (= D184), K202 (= K209)
binding pyridoxal-5'-phosphate: S79 (= S86), G80 (= G87), Q81 (= Q88), F105 (≠ Y112), D176 (= D184), T178 (= T186), S199 (= S206), T201 (= T208), K202 (= K209), A404 (= A425)

Query Sequence

>WP_004514768.1 NCBI__GCF_000012925.1:WP_004514768.1
MTDTKISFDTLALHAGQEPDPTTLSRAVPIYQTASYAFKSSEHAANLFGLKEFGNIYTRL
MNPTSDVLEKRLAELDGGVGALAVASGQAAITYAVLNIAQAGQNIVSTSYLYGGTYNLFH
YTLPKLGITVKFVDTSDPENVRRAIDDKTRLVYTESVGNPKNNVDDFEAIARIAHEAGIP
FVVDNTVTTSYLFKPLEHGADIVVYSLTKFIGGHGTSIGGAVVDGGKFPWNNGRFPEMTE
PDPSYHGLCYWEALGNLSYILKMRITLLRDMGACISPFNSFLFLQGLETLPVRMARHVEN
ARAVAQWLEKHPLVSWVNYPGLASHKDHGNARKYLPKGEGAIIGFGINGGLEAGRRFIDS
AKLLSHLANIGDAKSLVIHPASTTHQQLSEEEQLASGVTQDYIRLSIGIEEVADIIADID
QALAASQG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory