SitesBLAST
Comparing WP_004686588.1 NCBI__GCF_000022745.1:WP_004686588.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
7borA Structure of pseudomonas aeruginosa coa-bound odaa (see paper)
41% identity, 99% coverage: 2:248/250 of query aligns to 1:247/247 of 7borA
- active site: N63 (= N64), F68 (= F69), D77 (= D76), G81 (= G80), I105 (≠ V108), T108 (= T111), F128 (= F131), L133 (= L136), P135 (= P138), E136 (= E139), A222 (≠ T223), L232 (= L233)
- binding coenzyme a: D21 (≠ E22), K22 (= K23), A25 (= A26), S61 (= S62), I65 (≠ M66), V103 (≠ I106), F128 (= F131), L131 (= L134), F244 (= F245), R247 (= R248)
6slbAAA Enoyl-CoA hydratase/carnithine racemase (see paper)
38% identity, 83% coverage: 5:211/250 of query aligns to 5:211/257 of 6slbAAA
- active site: Q64 (≠ N64), F69 (= F69), L80 (≠ G80), N84 (≠ L84), A108 (≠ V108), S111 (≠ T111), A130 (≠ P130), F131 (= F131), L136 (= L136), P138 (= P138), D139 (≠ E139)
- binding (~{E})-6-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethylsulfanyl]-6-oxidanylidene-hex-3-enoic acid: R58 (≠ G58), A62 (≠ S62), Q64 (≠ N64), D65 (= D65), L66 (≠ M66), Y76 (≠ D76), A108 (≠ V108), F131 (= F131), D139 (≠ E139)
Sites not aligning to the query:
6slaAAA Enoyl-CoA hydratase/carnithine racemase (see paper)
38% identity, 83% coverage: 5:211/250 of query aligns to 2:199/245 of 6slaAAA
- active site: Q61 (≠ N64), L68 (≠ T77), N72 (≠ S81), A96 (≠ V108), S99 (≠ T111), A118 (≠ P130), F119 (= F131), L124 (= L136), P126 (= P138), N127 (≠ E139)
- binding ~{S}-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethyl] 2-(2,5-dihydrooxepin-7-yl)ethanethioate: L21 (≠ K24), A59 (≠ S62), Q61 (≠ N64), D62 (= D65), L63 (≠ M66), L68 (≠ T77), Y71 (≠ G80), A94 (≠ I106), G95 (= G107), A96 (≠ V108), F119 (= F131), I122 (≠ L134), L124 (= L136), N127 (≠ E139)
Sites not aligning to the query:
2hw5C The crystal structure of human enoyl-coenzyme a (coa) hydratase short chain 1, echs1
29% identity, 99% coverage: 3:250/250 of query aligns to 4:254/260 of 2hw5C
- active site: A68 (≠ N64), M73 (≠ F69), S83 (≠ G80), L87 (= L84), G111 (≠ V108), E114 (≠ T111), P133 (= P130), E134 (≠ F131), T139 (≠ L136), P141 (= P138), G142 (≠ E139), K227 (≠ T223), F237 (≠ L233)
- binding crotonyl coenzyme a: K26 (≠ E22), A27 (≠ K23), L28 (≠ K24), A30 (= A26), K62 (≠ G58), I70 (≠ M66), F109 (≠ I106)
1dubA 2-enoyl-coa hydratase, data collected at 100 k, ph 6.5 (see paper)
29% identity, 99% coverage: 3:250/250 of query aligns to 4:254/260 of 1dubA
- active site: A68 (≠ N64), M73 (= M70), S83 (≠ G80), L87 (= L84), G111 (≠ V108), E114 (≠ T111), P133 (= P130), E134 (≠ F131), T139 (≠ L136), P141 (= P138), G142 (≠ E139), K227 (≠ T223), F237 (≠ L233)
- binding acetoacetyl-coenzyme a: K26 (≠ E22), A27 (≠ K23), L28 (≠ K24), A30 (= A26), A66 (≠ S62), A68 (≠ N64), D69 (= D65), I70 (≠ M66), Y107 (≠ L104), G110 (= G107), G111 (≠ V108), E114 (≠ T111), P133 (= P130), E134 (≠ F131), L137 (= L134), G142 (≠ E139), F233 (= F229), F249 (= F245)
1ey3A Structure of enoyl-coa hydratase complexed with the substrate dac-coa (see paper)
29% identity, 99% coverage: 3:250/250 of query aligns to 2:252/258 of 1ey3A
- active site: A66 (≠ N64), M71 (= M70), S81 (≠ G80), L85 (= L84), G109 (≠ V108), E112 (≠ T111), P131 (= P130), E132 (≠ F131), T137 (≠ L136), P139 (= P138), G140 (≠ E139), K225 (≠ T223), F235 (≠ L233)
- binding 4-(n,n-dimethylamino)cinnamoyl-coa: K24 (≠ E22), L26 (≠ K24), A28 (= A26), A64 (≠ S62), G65 (= G63), A66 (≠ N64), D67 (= D65), I68 (≠ M66), L85 (= L84), W88 (≠ L87), G109 (≠ V108), P131 (= P130), L135 (= L134), G140 (≠ E139)
P14604 Enoyl-CoA hydratase, mitochondrial; mECH; mECH1; Enoyl-CoA hydratase 1; ECHS1; Short-chain enoyl-CoA hydratase; SCEH; EC 4.2.1.17; EC 5.3.3.8 from Rattus norvegicus (Rat) (see 3 papers)
29% identity, 99% coverage: 3:250/250 of query aligns to 34:284/290 of P14604
- E144 (≠ T111) mutation to D: Reduces activity 50-fold.; mutation to Q: Reduces activity 3300-fold.
- E164 (≠ F131) mutation to D: Reduces activity 1250-fold.; mutation to Q: Reduces activity 330000-fold.
Sites not aligning to the query:
- 1:29 modified: transit peptide, Mitochondrion
1mj3A Crystal structure analysis of rat enoyl-coa hydratase in complex with hexadienoyl-coa (see paper)
29% identity, 99% coverage: 3:250/250 of query aligns to 4:252/258 of 1mj3A
- active site: A68 (≠ N64), M73 (≠ F69), S83 (≠ F79), L85 (≠ S81), G109 (≠ V108), E112 (≠ T111), P131 (= P130), E132 (≠ F131), T137 (≠ L136), P139 (= P138), G140 (≠ E139), K225 (≠ T223), F235 (≠ L233)
- binding hexanoyl-coenzyme a: K26 (≠ E22), A27 (≠ K23), L28 (≠ K24), A30 (= A26), A66 (≠ S62), G67 (= G63), A68 (≠ N64), D69 (= D65), I70 (≠ M66), G109 (≠ V108), P131 (= P130), E132 (≠ F131), L135 (= L134), G140 (≠ E139)
2dubA Enoyl-coa hydratase complexed with octanoyl-coa (see paper)
28% identity, 99% coverage: 3:250/250 of query aligns to 3:248/254 of 2dubA
- active site: A67 (≠ N64), M72 (≠ F69), S82 (≠ D85), G105 (≠ V108), E108 (≠ T111), P127 (= P130), E128 (≠ F131), T133 (≠ L136), P135 (= P138), G136 (≠ E139), K221 (≠ T223), F231 (≠ L233)
- binding octanoyl-coenzyme a: K25 (≠ E22), A26 (≠ K23), L27 (≠ K24), A29 (= A26), A65 (≠ S62), A67 (≠ N64), D68 (= D65), I69 (≠ M66), K70 (≠ Q67), G105 (≠ V108), E108 (≠ T111), P127 (= P130), E128 (≠ F131), G136 (≠ E139), A137 (= A140)
5zaiC Crystal structure of 3-hydroxypropionyl-coa dehydratase from metallosphaera sedula (see paper)
26% identity, 99% coverage: 3:250/250 of query aligns to 4:253/259 of 5zaiC
- active site: A65 (≠ N64), F70 (= F69), S82 (≠ T77), R86 (≠ S81), G110 (≠ V108), E113 (≠ T111), P132 (= P130), E133 (≠ F131), I138 (≠ L136), P140 (= P138), G141 (≠ E139), A226 (≠ T223), F236 (≠ L233)
- binding coenzyme a: K24 (= K23), L25 (≠ K24), A63 (≠ S62), G64 (= G63), A65 (≠ N64), D66 (= D65), I67 (≠ M66), P132 (= P130), R166 (≠ G164), F248 (= F245), K251 (≠ R248)
Q9WTK2 Chromodomain Y-like protein; CDY-like; Crotonyl-CoA hydratase; Putative histone acetyltransferase Cdyl; EC 4.2.1.-; EC 2.3.1.48 from Mus musculus (Mouse) (see paper)
26% identity, 98% coverage: 5:249/250 of query aligns to 340:589/593 of Q9WTK2
- S516 (≠ Y177) mutation to A: Abolishes CoA-binding. No effect on transcriptional repressor activity.
- RK 588:589 (= RK 248:249) mutation to AA: Abolishes CoA-binding. No effect on transcriptional repressor activity.
Q9Y232 Chromodomain Y-like protein; CDY-like; Crotonyl-CoA hydratase; EC 4.2.1.- from Homo sapiens (Human) (see 5 papers)
26% identity, 98% coverage: 5:249/250 of query aligns to 345:594/598 of Q9Y232
- S521 (≠ Y177) mutation to A: Abolishes CoA-binding and ability to inhibit histone crotonylation.
Sites not aligning to the query:
- 2 T → A: in dbSNP:rs3812179
- 9 S → P: in dbSNP:rs3812178
- 48 V → A: in dbSNP:rs13196069
- 60 A → G: in dbSNP:rs28360500
- 61:309 Interaction with EZH2
- 135 modified: N6,N6,N6-trimethyllysine; by EHMT2; alternate; modified: N6,N6-dimethyllysine; by EHMT2; alternate; modified: N6-methyllysine; by EHMT2; alternate
- 205 S→A: No impact on recruitment to DNA double strand breaks.
3q0jC Crystal structure of the mycobacterium tuberculosis crotonase in complex with the inhibitor acetoacetylcoa
27% identity, 99% coverage: 3:249/250 of query aligns to 4:250/255 of 3q0jC
- active site: A65 (≠ N64), M70 (= M70), T80 (≠ G80), F84 (≠ L84), G108 (≠ V108), E111 (≠ T111), P130 (= P130), E131 (≠ F131), V136 (≠ L136), P138 (= P138), G139 (≠ E139), L224 (≠ T223), F234 (≠ L233)
- binding acetoacetyl-coenzyme a: Q23 (≠ E22), A24 (≠ K23), L25 (≠ K24), A27 (= A26), A63 (≠ S62), G64 (= G63), A65 (≠ N64), D66 (= D65), I67 (≠ M66), K68 (≠ Q67), M70 (= M70), F84 (≠ L84), G107 (= G107), G108 (≠ V108), E111 (≠ T111), P130 (= P130), E131 (≠ F131), P138 (= P138), G139 (≠ E139), M140 (≠ A140)
3q0gC Crystal structure of the mycobacterium tuberculosis crotonase bound to a reaction intermediate derived from crotonyl coa
27% identity, 99% coverage: 3:249/250 of query aligns to 4:250/255 of 3q0gC
- active site: A65 (≠ N64), M70 (= M70), T80 (≠ G80), F84 (≠ L84), G108 (≠ V108), E111 (≠ T111), P130 (= P130), E131 (≠ F131), V136 (≠ L136), P138 (= P138), G139 (≠ E139), L224 (≠ T223), F234 (≠ L233)
- binding coenzyme a: L25 (≠ K24), A63 (≠ S62), I67 (≠ M66), K68 (≠ Q67), Y104 (≠ L104), P130 (= P130), E131 (≠ F131), L134 (= L134)
3h81A Crystal structure of enoyl-coa hydratase from mycobacterium tuberculosis (see paper)
27% identity, 99% coverage: 3:249/250 of query aligns to 3:249/256 of 3h81A
- active site: A64 (≠ N64), M69 (= M70), T79 (≠ G80), F83 (≠ L84), G107 (≠ V108), E110 (≠ T111), P129 (= P130), E130 (≠ F131), V135 (≠ L136), P137 (= P138), G138 (≠ E139), L223 (≠ T223), F233 (≠ L233)
- binding calcium ion: F233 (≠ L233), Q238 (≠ A238)
3q0gD Crystal structure of the mycobacterium tuberculosis crotonase bound to a reaction intermediate derived from crotonyl coa
27% identity, 99% coverage: 3:249/250 of query aligns to 3:245/250 of 3q0gD
- active site: A64 (≠ N64), M69 (≠ F69), T75 (≠ G80), F79 (≠ L84), G103 (≠ V108), E106 (≠ T111), P125 (= P130), E126 (≠ F131), V131 (≠ L136), P133 (= P138), G134 (≠ E139), L219 (≠ T223), F229 (≠ L233)
- binding Butyryl Coenzyme A: F225 (= F229), F241 (= F245)
O53561 Enoyl-CoA hydratase EchA19; EC 4.2.1.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
28% identity, 98% coverage: 6:249/250 of query aligns to 11:259/266 of O53561
- K135 (≠ L126) modified: N6-succinyllysine; mutation to E: Nearly wild-type levels of succinylation in vitro, reduces specific activity 8-fold.
- 135:142 (vs. 126:133, 13% identical) mutation to EFGISEAE: Very low levels of succinylation in vitro, reduces specific activity 15-fold.
- K142 (≠ D133) modified: N6-succinyllysine; mutation to E: About 50% succinylation in vitro, reduces specific activity 7-fold.
Q9P4U9 Enoyl-CoA hydratase AKT3-1; AF-toxin biosynthesis protein 3-1; EC 4.2.1.17 from Alternaria alternata (Alternaria rot fungus) (Torula alternata) (see paper)
28% identity, 96% coverage: 12:250/250 of query aligns to 24:265/296 of Q9P4U9
Sites not aligning to the query:
- 294:296 Peroxisomal targeting signal type 1
Q8GYN9 1,4-dihydroxy-2-naphthoyl-CoA synthase, peroxisomal; DHNS; Enoyl-CoA hydratase/isomerase D; ECHID; Naphthoate synthase; EC 4.1.3.36 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
29% identity, 78% coverage: 16:211/250 of query aligns to 90:288/337 of Q8GYN9
Sites not aligning to the query:
- 20 H→V: Loss of peroxisomal targeting.
5dufA Crystal structure of m. Tuberculosis echa6 bound to ligand gsk729a (see paper)
31% identity, 84% coverage: 2:211/250 of query aligns to 1:201/245 of 5dufA
- active site: A62 (≠ N64), D67 (= D76), P74 (≠ L84), I78 (= I88), A102 (≠ V108), Q105 (≠ T111), P124 (= P130), T125 (≠ F131), L130 (= L136), L132 (≠ P138), D133 (≠ E139)
- binding (5R,7S)-5-(4-ethylphenyl)-7-(trifluoromethyl)-4,5,6,7-tetrahydropyrazolo[1,5-a]pyrimidine-3-carboxylic acid: L77 (= L87), I78 (= I88), H81 (≠ A91), D85 (vs. gap), Q105 (≠ T111), D133 (≠ E139), W135 (≠ G141)
Sites not aligning to the query:
Query Sequence
>WP_004686588.1 NCBI__GCF_000022745.1:WP_004686588.1
MSEHILIERKGAVQVIRLNRPEKKNAITRAMYAAMAKALKDGDADDAVRAHVFLGVPGAF
SSGNDMQDFMAAATGDTSFGSEILDFLIPLAETKKPIVSGVDGLAIGVGTTIHFHCDLTF
ATPRALFRTPFVDLGLVPEAGSSLLAPLLMGHQKAFALLALGHGFTAEAAQEAGIVYQIV
DEEALEAEVMKAAEEIAAKPPQAMQIARALMRLPAENIADRITREAKHFAERLTSDEARE
AVMAFLSRKK
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory