SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_004688980.1 NCBI__GCF_000022745.1:WP_004688980.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

6jw8A The crystal structure of kand2 in complex with nadh and 3"-deamino-3"- hydroxykanamycin b (see paper)
23% identity, 98% coverage: 8:356/356 of query aligns to 1:336/342 of 6jw8A

query
sites
6jw8A

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binding (2S,3R,4S,5S,6R)-2-[(1S,2S,3R,4S,6R)-3-[(2R,3R,4R,5S,6R)-6-(aminomethyl)-3-azanyl-4,5-bis(oxidanyl)oxan-2-yl]oxy-4,6-bis(azanyl)-2-oxidanyl-cyclohexyl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol: F11 (≠ N18), F152 (≠ L153), N154 (≠ Q155), D175 (= D177), L176 (≠ C178), H179 (= H181), E236 (= E240), W271 (≠ R285)
binding 1,4-dihydronicotinamide adenine dinucleotide: G10 (= G17), F11 (≠ N18), M12 (= M19), D33 (≠ N40), L34 (≠ R41), T70 (≠ A74), T71 (= T75), P72 (≠ Y76), N73 (≠ S77), L75 (≠ S79), H76 (= H80), Q79 (≠ Y83), E93 (= E97), K94 (= K98), N122 (≠ I126), W161 (= W162), H179 (= H181), Y290 (≠ P304)

6jw7A The crystal structure of kand2 in complex with nadh and 3"-deamino-3"- hydroxykanamycin a (see paper)
23% identity, 98% coverage: 8:356/356 of query aligns to 1:336/342 of 6jw7A

query
sites
6jw7A

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binding (2R,3S,4S,5R,6R)-2-(aminomethyl)-6-[(1R,2S,3S,4R,6S)-4,6-bis(azanyl)-3-[(2S,3R,4S,5S,6R)-6-(hydroxymethyl)-3,4,5-tris(oxidanyl)oxan-2-yl]oxy-2-oxidanyl-cyclohexyl]oxy-oxane-3,4,5-triol: F11 (≠ N18), N154 (≠ Q155), D175 (= D177), H179 (= H181), E236 (= E240), W271 (≠ R285)
binding 1,4-dihydronicotinamide adenine dinucleotide: G8 (= G15), G10 (= G17), F11 (≠ N18), M12 (= M19), D33 (≠ N40), L34 (≠ R41), T70 (≠ A74), T71 (= T75), P72 (≠ Y76), N73 (≠ S77), L75 (≠ S79), H76 (= H80), Q79 (≠ Y83), E93 (= E97), K94 (= K98), N122 (≠ I126), W161 (= W162), Y290 (≠ P304)

6jw6A The crystal structure of kand2 in complex with NAD (see paper)
23% identity, 98% coverage: 8:356/356 of query aligns to 1:336/341 of 6jw6A

query
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6jw6A

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binding nicotinamide-adenine-dinucleotide: G10 (= G17), F11 (≠ N18), M12 (= M19), D33 (≠ N40), L34 (≠ R41), T70 (≠ A74), T71 (= T75), P72 (≠ Y76), N73 (≠ S77), L75 (≠ S79), H76 (= H80), Q79 (≠ Y83), E93 (= E97), K94 (= K98), N122 (≠ I126), W161 (= W162), H179 (= H181), Y290 (≠ P304)

1evjA Crystal structure of glucose-fructose oxidoreductase (gfor) delta1-22 s64d (see paper)
31% identity, 40% coverage: 56:198/356 of query aligns to 58:205/340 of 1evjA

query
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1evjA

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L

V

M

I

I

G

G

Y

Y

I
x
R

L

C

R

H

H

Y

H

D

P

P

-

M

-

N

-

R

S

A

W

A

M

V

R

K

L

L

I

I

E

R

E

E

A

N

R

Q

-

L

-

G

K

K

L

L

G

G

G

M

P

V

Y

T

V

T

F

D

R

N

M

S

N

D

-

V

L

M

N

D

Q

Q

Q

N

S

D

S

P

G

A

A

Q

T

Q

W
|
W

E
x
R

T

L

H

R

K

R

N

E

L

L

M

A

N

G

T

G

T

G

S

S

P

-

I

L

V

M

D

D

C

I

G

G

V

I

H
x
Y

Y

G

V

L

D

N

V

G

M

T

C

R

Q

Y

I

L

T

L

D

G

A

E

R

E

P

P

V

I

E

E

V

V

R

R

active site: K100 (= K98), Y188 (≠ H181)
binding nicotinamide-adenine-dinucleotide: L77 (≠ T75), P78 (≠ Y76), N79 (≠ S77), H82 (= H80), E99 (= E97), K100 (= K98), R128 (≠ I126), W170 (= W162), R171 (≠ E163), Y188 (≠ H181)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 9, 10, 11, 12, 13, 35, 267

1rydA Crystal structure of glucose-fructose oxidoreductase from zymomonas mobilis
32% identity, 40% coverage: 56:198/356 of query aligns to 87:234/381 of 1rydA

query
sites
1rydA

H
x
Y

P

S

S

N

F

F

H

D

E

K

A

I

L

A

K

K

E

D

L

P

K

K

P

I

D

D

L

A

A

V

C

Y

V

I

A

I

T
x
L

Y
x
P

S
x
N

E

S

S
x
L

H
|
H

A

A

E

E

Y

F

A

A

I

I

A

R

A

A

M

F

E

K

Q

A

G

G

A

K

D

H

V

V

F

M

V

C

E
|
E

K
|
K

P

P

L

M

A

A

T

T

T

S

V

V

E

A

D

D

A

C

R

Q

R

R

V

M

I

I

D

D

C

A

A

A

R

K

A

A

N

A

G

N

R

K

K

K

L

L

V

M

I

I

G

G

Y

Y

I
x
R

L

C

R

H

H

Y

H

D

P

P

-

M

-

N

-

R

S

A

W

A

M

V

R

K

L

L

I

I

E

R

E

E

A

N

R

Q

-

L

-

G

K

K

L

L

G

G

G

-

P

-

Y

M

V

V

F

T

R

T

M

D

N

N

-

S

-

D

-

V

L

M

N

D

Q

Q

Q

N

S

D

S

P

G
x
A

A

Q

T

Q

W
|
W

E
x
R

T

L

H

R

K

R

N

E

L

L

M

A

N

G

T

G

T

G

S

S

P

-

I

L

V

M

D

D

C

I

G

G

V

I

H
x
Y

Y

G

V

L

D

N

V

G

M

T

C

R

Q

Y

I

L

T

L

D

G

A

E

R

E

P

P

V

I

E

E

V

V

R

R

active site: K129 (= K98), Y217 (≠ H181)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: Y87 (≠ H56), L106 (≠ T75), P107 (≠ Y76), N108 (≠ S77), L110 (≠ S79), H111 (= H80), E128 (= E97), K129 (= K98), R157 (≠ I126), A196 (≠ G159), W199 (= W162), R200 (≠ E163), Y217 (≠ H181)

Sites not aligning to the query:

1h6dA Oxidized precursor form of glucose-fructose oxidoreductase from zymomonas mobilis complexed with glycerol (see paper)
32% identity, 40% coverage: 56:198/356 of query aligns to 89:236/383 of 1h6dA

query
sites
1h6dA

H
x
Y

P

S

S

N

F

F

H

D

E

K

A

I

L

A

K

K

E

D

L

P

K

K

P

I

D

D

L

A

A

V

C

Y

V

I

A
x
I

T
x
L

Y
x
P

S
x
N

E

S

S

L

H
|
H

A

A

E

E

Y

F

A

A

I

I

A

R

A

A

M

F

E

K

Q

A

G

G

A

K

D

H

V

V

F

M

V

C

E
|
E

K
|
K

P

P

L

M

A

A

T

T

T

S

V

V

E

A

D

D

A

C

R

Q

R

R

V

M

I

I

D

D

C

A

A

A

R

K

A

A

N

A

G

N

R

K

K

K

L

L

V

M

I

I

G

G

Y

Y

I
x
R

L

C

R

H

H

Y

H

D

P

P

-

M

-

N

-

R

S

A

W

A

M

V

R

K

L

L

I

I

E

R

E

E

A

N

R

Q

-

L

-

G

K

K

L

L

G

G

G

-

P

-

Y

M

V

V

F

T

R

T

M

D

N

N

-

S

-

D

-

V

L

M

N

D

Q

Q

Q

N

S

D

S

P

G
x
A

A

Q

T

Q

W
|
W

E
x
R

T

L

H

R

K

R

N

E

L

L

M

A

N

G

T

G

T

G

S

S

P

-

I

L

V

M

D
|
D

C

I

G

G

V

I

H
x
Y

Y

G

V

L

D

N

V

G

M

T

C

R

Q

Y

I

L

T

L

D

G

A

E

R

E

P

P

V

I

E

E

V

V

R

R

active site: K131 (= K98), Y219 (≠ H181)
binding glycerol: K131 (= K98), R202 (≠ E163), D215 (= D177), Y219 (≠ H181)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: Y89 (≠ H56), I107 (≠ A74), L108 (≠ T75), P109 (≠ Y76), N110 (≠ S77), H113 (= H80), E130 (= E97), K131 (= K98), R159 (≠ I126), A198 (≠ G159), W201 (= W162), R202 (≠ E163), Y219 (≠ H181)

Sites not aligning to the query:

binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 17, 18, 19, 40, 41, 42, 43, 44, 66, 67, 71, 298

3ec7A Crystal structure of putative dehydrogenase from salmonella typhimurium lt2
27% identity, 48% coverage: 15:186/356 of query aligns to 9:181/336 of 3ec7A

query
sites
3ec7A

G

G

L

I

G
|
G

N
x
M

M
x
I

G

G

R

S

S

D

H

H

A

L

-

R

-

R

L

L

A

A

Y

-

H

N

R

T

N

V

P

S

G

G

F

V

E

E

I

V

V

V

G

A

L

V

V

C

N
x
D

R
x
I

T

V

K

A

V

G

P
x
R

V

A

P

Q

D

A

E

A

L

L

A

D

G

K

Y

Y

I

A

I

I

-

E

-

A

-

K

-

D

H

Y

P

N

S

D

F

Y

H

H

E

D

A

L

L

I

K

N

E

D

L

K

K

D

P

V

D

E

L

V

A

V

C

I

V

I

A
x
T

T
x
A

Y
x
S

S
x
N

E

E

S

A

H
|
H

A

A

E

D

Y

V

A

A

I

V

A

A

A

A

M

L

E

N

Q

A

G

N

A

K

D

Y

V

V

F

F

V

C

E
|
E

K
|
K

P

P

L

L

A

A

T

V

T

T

V

A

E

A

D

D

A

C

R

Q

R

R

V

V

I

I

D

E

C

A

A

E

R

Q

A

K

N

N

G

G

R

K

K

R

L

M

V

V

-

Q

I

I

G

G

Y

F

I
x
M

L

R

R

R

H

Y

H

D

P

K

S

G

W

Y

M

V

R

Q

L

L

-

K

-

N

I

I

E

I

E

D

A

S

R

G

K

E

L

I

G

G

G

Q

P

P

Y

L

V

M

F

V

R

H

M

G

N

R

L

H

N

Y

Q

N

Q

A

S

S

S

T

G

V

A

P

T

E

W

Y

E

K

T

T

H

P

K

Q

N

A

L

I

M

Y

N

E

T

T

T

L

S

-

P

-

I

-

V

-

D

-

C

-

G

-

V

I

H
|
H

Y

E

V

I

D

D

V

V

M

M

active site: K97 (= K98), H176 (= H181)
binding nicotinamide-adenine-dinucleotide: G11 (= G17), M12 (≠ N18), I13 (≠ M19), D35 (≠ N40), I36 (≠ R41), R40 (≠ P45), T73 (≠ A74), A74 (≠ T75), S75 (≠ Y76), N76 (≠ S77), H79 (= H80), E96 (= E97), K97 (= K98), M126 (≠ I126)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 271, 279

9azoA Crystal structure of chms dehydrogenase pmdc from comamonas testosteroni bound to cofactor NADP (see paper)
31% identity, 44% coverage: 9:165/356 of query aligns to 3:171/315 of 9azoA

query
sites
9azoA

V

I

R

K

V

V

L

A

S

L

A

A

G
|
G

L

A

G
|
G

N
x
A

M
x
F

G

G

R

I

S

K

H

H

A

L

L

D

A

G

Y

I

H

K

R

N

N

I

P

D

G

G

F

V

E

E

I

V

V

V

G

S

L

L

V

V

N
x
G

R
|
R

-

R

-

F

-

D

-

Q

T

T

K

K

V

-

P

E

V

V

P

A

D

D

E

K

L

Y

A

G

G

I

Y

A

I

H

I

V

H

A

P

T

S

D

F

L

H

A

E

E

A

S

L

L

K

A

E

L

L

P

K

E

P

V

D

D

L

A

A

V

C

I

V

L

A
x
C

T
|
T

Y

P

S
x
T

E

Q

S

M

H
|
H

A

A

E

E

Y

Q

A

A

I

I

A

A

A

C

M

M

E

K

Q

A

G

G

A

K

D

H

V

V

F

Q

V

V

E
|
E

K
x
I

P

P

L

L

A

A

T

D

T

A

V

L

E

K

D

D

A

A

R

Q

R

E

V

V

I

A

D

E

C

L

A

Q

R

K

A

Q

N

T

G

G

R

L

K

V

L

A

V

M

I

V

G

G

Y

H

I

T

L

R

R

R

H

F

H

N

P

P

S

S

-

H

-

Q

W

W

M

V

R

H

L

K

I

K

E

I

E

E

A

A

R

G

K

E

L

F

G

N

-

I

-

Q

-

Q

-

M

-

D

-

V

G

Q

P

T

Y

Y

V

F

F

F

-

R

R

R

M

T

N

N

L

M

N
|
N

Q

A

Q

L

S

G

S

Q

G

A

A
x
R

T

S

W
|
W

E

T

T

D

H

H

binding nadp nicotinamide-adenine-dinucleotide phosphate: G9 (= G15), G11 (= G17), A12 (≠ N18), F13 (≠ M19), G34 (≠ N40), R35 (= R41), C71 (≠ A74), T72 (= T75), T74 (≠ S77), H77 (= H80), E94 (= E97), I95 (≠ K98), N160 (= N154), R166 (≠ A160), W168 (= W162)

Sites not aligning to the query:

binding nadp nicotinamide-adenine-dinucleotide phosphate: 175, 179

7xr9A Crystal structure of dgpa with glucose (see paper)
27% identity, 38% coverage: 52:185/356 of query aligns to 45:181/344 of 7xr9A

query
sites
7xr9A

G

G

Y

A

I

Q

I

V

H

T

P

A

S
x
D

F
x
Y

H

K

E

E

A

L

L

L

K

A

E

N

L

P

K

E

P

V

D

E

L

V

A

V

C

H

V

V

A
x
C

T
|
T

Y
x
P

S

N

E

V

S

S

H
|
H

A

S

E

E

Y

I

A

T

I

I

A

A

A

A

M

F

E

E

Q

A

G

G

A

K

D

H

V

V

F

Y

V

C

E
|
E

K
|
K

P
|
P

L

M

A

S

T

H

T

S

V

T

E

E

D

E

A

A

R

E

R

K

V

M

I

V

D

E

C

A

A

W

R

K

A

K

N

S

G

G

R

K

K

Q

L

F

V

T

I

I

G

G

Y

Y

I
x
Q

L

N

R

R

H

F

H

R

P

E

S

E

W

V

M

M

R

N

L

L

I

K

E

K

E

S

A

C

R

D

K

K

-

G

-

E

L

L

G

G

G

E

P

I

Y

Y

V

Y

F

G

R

K

M

A

N

H

L

A

N

V

Q
x
R

Q

R

S

R

S

A

G

V

A

P

T

T

W
|
W

E

G

T
x
V

H
x
F

K

M

N

D

L

K

-

E

M

A

N
x
Q

T

G

T

G

S

G

P

P

I

L

V

I

D
|
D

C

I

G

G

V

T

H
|
H

Y

A

V

L

D

D

V

I

binding beta-D-glucopyranose: K91 (= K98), R150 (≠ Q155), W157 (= W162), F160 (≠ H165), D173 (= D177), H177 (= H181)
binding nicotinamide-adenine-dinucleotide: D51 (≠ S58), Y52 (≠ F59), C67 (≠ A74), T68 (= T75), P69 (≠ Y76), H73 (= H80), E90 (= E97), K91 (= K98), P92 (= P99), Q119 (≠ I126), V159 (≠ T164), F160 (≠ H165), Q166 (≠ N170)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 13, 14, 15

7xreC Crystal structure of dgpa (see paper)
27% identity, 37% coverage: 55:185/356 of query aligns to 58:191/363 of 7xreC

query
sites
7xreC

I

V

H

T

P

A

S

D

F

Y

H

K

E

E

A

L

L

L

K

A

E

N

L

P

K

E

P

V

D

E

L

V

A

V

C

H

V

V

A

C

T
|
T

Y
x
P

S
x
N

E

V

S

S

H
|
H

A

S

E

E

Y

I

A

T

I

I

A

A

A

A

M

F

E

E

Q

A

G

G

A

K

D

H

V

V

F

Y

V

C

E
|
E

K
|
K

P
|
P

L

M

A

S

T

H

T

S

V

T

E

E

D

E

A

A

R

E

R

K

V

M

I

V

D

E

C

A

A

W

R

K

A

K

N

S

G

G

R

K

K

Q

L

F

V

T

I

I

G

G

Y

Y

I

Q

L

N

R

R

H

F

H

R

P

E

S

E

W

V

M

M

R

N

L

L

I

K

E

K

E

S

A

C

R

D

K

K

-

G

-

E

L

L

G

G

G

E

P

I

Y

Y

V

Y

F

G

R

K

M

A

N

H

L

A

N

V

Q

R

Q

R

S

R

S

A

G

V

A

P

T

T

W

W

E

G

T

V

H
x
F

K

M

N

D

L

K

-

E

M

A

N
x
Q

T

G

T

G

S

G

P

P

I

L

V

I

D

D

C

I

G

G

V

T

H
|
H

Y

A

V

L

D

D

V

I

binding nicotinamide-adenine-dinucleotide: T78 (= T75), P79 (≠ Y76), N80 (≠ S77), H83 (= H80), E100 (= E97), K101 (= K98), P102 (= P99), F170 (≠ H165), Q176 (≠ N170), H187 (= H181)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 13, 14, 38, 39, 313, 314

3e18A Crystal structure of NAD-binding protein from listeria innocua
24% identity, 67% coverage: 10:246/356 of query aligns to 5:240/348 of 3e18A

query
sites
3e18A

R

Q

V

L

L

V

S

I

A

V

G

G

L

Y

G
|
G

N
x
G

M
|
M

G

G

R

S

S

Y

H

H

A

V

L

T

A

L

Y

A

H

S

R

A

N

A

P

D

G

N

F

L

E

E

I

V

V

H

G

G

L

V

V
x
F

N
x
D

R
x
I

T

-

K

-

V

L

P

A

V

E

P
x
K

D

R

E

E

L

A

A

A

-

A

-

Q

-

K

G

G

Y

L

I

K

I

I

H

Y

P

E

S

S

F

Y

H

E

E

A

A

V

L

L

K

A

E

D

L

E

K

K

P

V

D

D

L

A

A

V

C

L

V

I

A
|
A

T
|
T

Y
x
P

S
x
N

E

D

S

S

H
|
H

A

K

E

E

Y

L

A

A

I

I

A

S

A

A

M

L

E

E

Q

A

G

G

A

K

D

H

V

V

F

V

V

C

E
|
E

K
|
K

P

P

L

V

A

T

T

M

T

T

V

S

E

E

D

D

A

L

R

L

R

A

V

I

I

M

D

D

C

V

A

A

R

K

A

R

N

V

G

N

R

K

K

H

L

F

V

M

I

V

G

H

Y

Q

I
x
N

L

R

R

R

H

W

H

D

P

E

S

D

W

F

M

L

R

I

L

I

I

K

E

E

-

M

-

F

E

E

A

Q

R

K

K

T

L

I

G

G

G

E

P

M

Y

F

V

-

F

-

R

-

M

-

N

H

L

L

N

E

Q

S

Q

R

S

V

S

H

G

G

A

A

T

N

-

G

-

I

-

P

-

G

-

D

W
|
W

E
x
R

T

H

H

L

K

K

N

-

L

-

M

A

N

H

T

G

T

G

S

G

P

M

I

V

V

L

D

D

C

W

G

G

V

V

H
|
H

Y

L

V

L

D

D

V

Q

M

L

C

L

Q

F

I

L

T

V

D

D

A

S

R

N

P

V

V

K

E

S

V

V

R

S

G

A

-

N

M

L

G

S

L

F

R

A

L

L

S

G

D

D

E

E

I

V

K

D

P

D

D

G

M

F

Y

V

N

T

Y

F

G

-

H

-

L

-

Q

-

V

I

L

T

F

F

D

E

D

N

G

G

T

I

V

T

G

A

W

Q

Y

I

E

E

A

V

G

G

W

T

G

T

P

N

M

F

M

I

S

K

E

L

T

P

A

R

F

W

F

Y

V

V

K

K

active site: K94 (= K98), H178 (= H181)
binding nicotinamide-adenine-dinucleotide: G12 (= G17), G13 (≠ N18), M14 (= M19), F34 (≠ V39), D35 (≠ N40), I36 (≠ R41), K40 (≠ P47), A70 (= A74), T71 (= T75), P72 (≠ Y76), N73 (≠ S77), H76 (= H80), E93 (= E97), K94 (= K98), N122 (≠ I126), W161 (= W162), R162 (≠ E163), H178 (= H181)

4mjlD Crystal structure of myo-inositol dehydrogenase from lactobacillus casei in complex with NAD and d-chiro-inositol
27% identity, 54% coverage: 9:202/356 of query aligns to 3:208/339 of 4mjlD

query
sites
4mjlD

V

V

R

K

V

V

L

G

S

V

A

I

G

G

L

T

G
|
G

N
x
A

M
|
M

G

G

R

R

S

A

H

H

A

I

-

D

L

R

A

L

Y

T

H

N

R

V

N

L

P

T

G

G

F

A

E

E

I

V

V

V

G

A

L

V

V

T

N
x
D

-
x
I

-

D

-

H

-

E

R

A

T

A

K

E

V

A

P

A

V

V

P

R

D

D

E

F

L

H

A

L

G

N

Y

A

I

K

I

V

H

Y

P

P

S

D

F

D

H

T

E

S

A

L

L

L

K

Q

E

D

L

P

K

D

P

I

D

D

L

A

A

V

C

F

V

V

A
x
V

T
x
S

Y

F

S

G

E

G

S

A

H
|
H

A

E

E

A

Y

T

A

V

I

L

A

K

A

A

M

L

E

D

Q

T

G

D

A

K

D

F

V

I

F

F

V

T

E
|
E

K
|
K

P

P

L

L

A

A

T

T

T

T

V

L

E

E

D

G

A

A

R

K

R

R

V

I

I

V

D

D

C

K

A

E

R

L

A

T

N

K

G

S

R

K

K

K

L

V

V

I

I

Q

G

V

Y

G

I

F

L
x
M

R

R

H

R

H

Y

P

D

S

Q

W

G

M

I

R

R

L

A

I

L

E

K

E

E

A

K

R

L

K

D

-

T

-

G

-

I

L

I

G

G

G

A

P

P

Y

L

V

V

F

V

R

R

-

A

-

S

-
x
H

M

I

N
|
N

L

P

N

N

Q

V

Q

A

S

S

S

N

G

-

A

-

T

-

W

-

E

-

T

-

H

Y

K

S

N

N

L

E

M

M

N

-

T

-

T

-

S

-

P

A

I

I

V

T

D
|
D

C
x
T

G

L

V

I

H
|
H

Y

E

V

I

D

D

V

E

M

M

-

H

-

W

-

L

-

L

-

D

-

D

-

E

-

Y

-

T

-

S

C

I

Q

Q

I

I

T

T

D

Y

A

P

R

R

-

Q

P

S

V

A

E

E

V

V

R

R

G

N

M

E

G

G

L

L

active site: K97 (= K98)
binding (1r,2r,3s,4s,5s,6s)-cyclohexane-1,2,3,4,5,6-hexol: K97 (= K98), H155 (vs. gap), N157 (= N152), D172 (= D177), T173 (≠ C178), H176 (= H181)
binding nicotinamide-adenine-dinucleotide: G11 (= G17), A12 (≠ N18), M13 (= M19), D35 (≠ N40), I36 (vs. gap), V73 (≠ A74), S74 (≠ T75), H79 (= H80), E96 (= E97), K97 (= K98), M126 (≠ L127), D172 (= D177)

Sites not aligning to the query:

4mioD Crystal structure of myo-inositol dehydrogenase from lactobacillus casei in complex with NAD(h) and myo-inositol
27% identity, 54% coverage: 9:202/356 of query aligns to 3:208/339 of 4mioD

query
sites
4mioD

V

V

R

K

V

V

L

G

S

V

A

I

G

G

L

T

G

G

N
x
A

M
|
M

G

G

R

R

S

A

H

H

A

I

-

D

L

R

A

L

Y

T

H

N

R

V

N

L

P

T

G

G

F

A

E

E

I

V

V

V

G

A

L

V

V

T

N
x
D

-
x
I

-

D

-

H

-

E

R

A

T

A

K

E

V

A

P

A

V

V

P

R

D

D

E

F

L

H

A

L

G

N

Y

A

I

K

I

V

H

Y

P

P

S

D

F

D

H

T

E

S

A

L

L

L

K

Q

E

D

L

P

K

D

P

I

D

D

L

A

A

V

C

F

V

V

A

V

T
x
S

Y

F

S

G

E

G

S
x
A

H
|
H

A

E

E

A

Y

T

A

V

I

L

A

K

A

A

M

L

E

D

Q

T

G

D

A

K

D

F

V

I

F

F

V

T

E
|
E

K
|
K

P

P

L

L

A

A

T

T

T

T

V

L

E

E

D

G

A

A

R

K

R

R

V

I

I

V

D

D

C

K

A

E

R

L

A

T

N

K

G

S

R

K

K

K

L

V

V

I

I

Q

G

V

Y

G

I

F

L
x
M

R

R

H

R

H

Y

P

D

S

Q

W

G

M

I

R

R

L

A

I

L

E

K

E

E

A

K

R

L

K

D

-

T

-

G

-

I

L

I

G

G

G

A

P

P

Y

L

V

V

F

V

R

R

-

A

-

S

-
x
H

M

I

N
|
N

L

P

N

N

Q

V

Q

A

S

S

S

N

G

-

A

-

T

-

W

-

E

-

T

-

H

Y

K

S

N

N

L

E

M

M

N

-

T

-

T

-

S

-

P

A

I

I

V

T

D
|
D

C
x
T

G

L

V

I

H
|
H

Y

E

V

I

D

D

V

E

M

M

-

H

-

W

-

L

-

L

-

D

-

D

-

E

-

Y

-

T

-

S

C

I

Q

Q

I

I

T

T

D

Y

A

P

R

R

-

Q

P

S

V

A

E

E

V

V

R

R

G

N

M

E

G

G

L

L

active site: K97 (= K98)
binding 1,2,3,4,5,6-hexahydroxy-cyclohexane: K97 (= K98), H155 (vs. gap), N157 (= N152), D172 (= D177), T173 (≠ C178), H176 (= H181)
binding 1,4-dihydronicotinamide adenine dinucleotide: A12 (≠ N18), M13 (= M19), D35 (≠ N40), I36 (vs. gap), S74 (≠ T75), A78 (≠ S79), H79 (= H80), E96 (= E97), K97 (= K98), M126 (≠ L127), D172 (= D177)

Sites not aligning to the query:

4mioA Crystal structure of myo-inositol dehydrogenase from lactobacillus casei in complex with NAD(h) and myo-inositol
27% identity, 54% coverage: 9:202/356 of query aligns to 3:208/339 of 4mioA

query
sites
4mioA

V

V

R

K

V

V

L

G

S

V

A

I

G

G

L

T

G
|
G

N
x
A

M
|
M

G

G

R

R

S

A

H

H

A

I

-

D

L

R

A

L

Y

T

H

N

R

V

N

L

P

T

G

G

F

A

E

E

I

V

V

V

G

A

L

V

V

T

N
x
D

-
x
I

-

D

-

H

-

E

R

A

T

A

K

E

V

A

P

A

V

V

P

R

D

D

E

F

L

H

A

L

G

N

Y

A

I

K

I

V

H

Y

P

P

S

D

F

D

H

T

E

S

A

L

L

L

K

Q

E

D

L

P

K

D

P

I

D

D

L

A

A

V

C

F

V

V

A

V

T
x
S

Y
x
F

S

G

E

G

S

A

H
|
H

A

E

E

A

Y

T

A

V

I

L

A

K

A

A

M

L

E

D

Q

T

G

D

A

K

D

F

V

I

F

F

V

T

E
|
E

K
|
K

P

P

L

L

A

A

T

T

T

T

V

L

E

E

D

G

A

A

R

K

R

R

V

I

I

V

D

D

C

K

A

E

R

L

A

T

N

K

G

S

R

K

K

K

L

V

V

I

I

Q

G

V

Y

G

I

F

L

M

R

R

H

R

H

Y

P

D

S

Q

W

G

M

I

R

R

L

A

I

L

E

K

E

E

A

K

R

L

K

D

-

T

-

G

-

I

L

I

G

G

G

A

P

P

Y

L

V

V

F

V

R

R

-

A

-

S

-

H

M

I

N

N

L

P

N

N

Q

V

Q

A

S

S

S

N

G

-

A

-

T

-

W

-

E

-

T

-

H

Y

K

S

N

N

L

E

M

M

N

-

T

-

T

-

S

-

P

A

I

I

V

T

D

D

C

T

G

L

V

I

H

H

Y

E

V

I

D

D

V

E

M

M

-

H

-

W

-

L

-

L

-

D

-

D

-

E

-

Y

-

T

-

S

C

I

Q

Q

I

I

T

T

D

Y

A

P

R

R

-

Q

P

S

V

A

E

E

V

V

R

R

G

N

M

E

G

G

L

L

active site: K97 (= K98)
binding 1,4-dihydronicotinamide adenine dinucleotide: G11 (= G17), A12 (≠ N18), M13 (= M19), D35 (≠ N40), I36 (vs. gap), S74 (≠ T75), F75 (≠ Y76), H79 (= H80), E96 (= E97), K97 (= K98)

Sites not aligning to the query:

binding 1,4-dihydronicotinamide adenine dinucleotide: 274, 282

4minA Crystal structure of myo-inositol dehydrogenase from lactobacillus casei with bound cofactor NAD
27% identity, 54% coverage: 9:202/356 of query aligns to 3:208/339 of 4minA

query
sites
4minA

V

V

R

K

V

V

L

G

S

V

A

I

G

G

L

T

G
|
G

N
x
A

M
|
M

G

G

R

R

S

A

H

H

A

I

-

D

L

R

A

L

Y

T

H

N

R

V

N

L

P

T

G

G

F

A

E

E

I

V

V

V

G

A

L

V

V

T

N
x
D

-
x
I

-

D

-

H

-

E

R

A

T

A

K

E

V

A

P

A

V

V

P

R

D

D

E

F

L

H

A

L

G

N

Y

A

I

K

I

V

H

Y

P

P

S

D

F

D

H

T

E

S

A

L

L

L

K

Q

E

D

L

P

K

D

P

I

D

D

L

A

A

V

C

F

V

V

A
x
V

T
x
S

Y
x
F

S

G

E

G

S

A

H
|
H

A

E

E

A

Y

T

A

V

I

L

A

K

A

A

M

L

E

D

Q

T

G

D

A

K

D

F

V

I

F

F

V

T

E
|
E

K
|
K

P

P

L

L

A

A

T

T

T

T

V

L

E

E

D

G

A

A

R

K

R

R

V

I

I

V

D

D

C

K

A

E

R

L

A

T

N

K

G

S

R

K

K

K

L

V

V

I

I

Q

G

V

Y

G

I

F

L

M

R

R

H

R

H

Y

P

D

S

Q

W

G

M

I

R

R

L

A

I

L

E

K

E

E

A

K

R

L

K

D

-

T

-

G

-

I

L

I

G

G

G

A

P

P

Y

L

V

V

F

V

R

R

-

A

-

S

-

H

M

I

N

N

L

P

N

N

Q

V

Q

A

S

S

S

N

G

-

A

-

T

-

W

-

E

-

T

-

H

Y

K

S

N

N

L

E

M

M

N

-

T

-

T

-

S

-

P

A

I

I

V

T

D

D

C

T

G

L

V

I

H

H

Y

E

V

I

D

D

V

E

M

M

-

H

-

W

-

L

-

L

-

D

-

D

-

E

-

Y

-

T

-

S

C

I

Q

Q

I

I

T

T

D

Y

A

P

R

R

-

Q

P

S

V

A

E

E

V

V

R

R

G

N

M

E

G

G

L

L

active site: K97 (= K98)
binding nicotinamide-adenine-dinucleotide: G11 (= G17), A12 (≠ N18), M13 (= M19), D35 (≠ N40), I36 (vs. gap), V73 (≠ A74), S74 (≠ T75), F75 (≠ Y76), H79 (= H80), E96 (= E97), K97 (= K98)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 282

6t2bB Glycoside hydrolase family 109 from akkermansia muciniphila in complex with galnac and NAD+.
33% identity, 31% coverage: 9:117/356 of query aligns to 34:154/439 of 6t2bB

query
sites
6t2bB

V

I

R

R

V

L

L

A

S

F

A

I

G
|
G

L

V

G

G

N
x
G

M
x
R

G

G

R

F

S

S

H

H

A

L

L

A

A

Q

Y

M

H

C

R

V

N

M

P

D

G

G

F

V

E

E

I

I

V

V

G

G

L

I

V

C

N
x
D

R
x
L

-

K

-

E

-

E

-

L

T

T

K

K

V

R

P

G

V

V

P

D

D

R

E

V

L

L

A

S

G

R

Y

M

I

G

I

K

H

S

P

P

-

L

-

G

-

Y

-

S

-

G

-

G

-
x
D

-

M

S

E

F

Y

H

L

E

T

A

M

L

L

K

K

E

E

L

L

K

K

P

P

D

D

L

A

A

V

C

I

V

I

A
x
S

T
|
T

Y

D

S
x
W

E

S

S

S

H
|
H

A

A

E

R

Y

I

A

A

I

C

A

D

A

S

M

M

E

K

Q

H

G

G

A

A

D

H

V

A

F

F

V

V

E
|
E

K
x
V

P

P

L

L

A

A

T

V

T

S

V

L

E

E

D

E

A

L

R

W

R

S

V

L

I

V

D

D

C

T

A

S

R

E

A

A

N

T

binding nicotinamide-adenine-dinucleotide: G40 (= G15), G43 (≠ N18), R44 (≠ M19), D65 (≠ N40), L66 (≠ R41), D93 (vs. gap), S111 (≠ A74), T112 (= T75), W114 (≠ S77), H117 (= H80), E134 (= E97), V135 (≠ K98)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 163, 205, 206, 209, 222, 225, 370

4l8vA Crystal structure of a12k/d35s mutant myo-inositol dehydrogenase from bacillus subtilis with bound cofactor NADP (see paper)
24% identity, 50% coverage: 9:186/356 of query aligns to 3:181/337 of 4l8vA

query
sites
4l8vA

V

L

R

R

V

I

L

G

S

V

A

I

G

G

L

T

G

G

N
x
K

M
x
I

G

G

R

K

S

E

H

H

A

I

-

N

L

R

A

I

Y

T

H

N

R

K

N

L

P

S

G

G

F

A

E

E

I

I

V

V

G

A

L

V

V

T

N

S

R

V

T

N

K

Q

V

E

P

A

V

A

P

Q

D

K

E

V

L

V

A

E

G

Q

Y

Y

-

Q

-

L

-

N

-

A

I

T

I

V

H

Y

P

P

S

N

F

D

H

D

E

S

A

L

L

L

K

A

E

D

L

E

K

N

P

V

D

D

L

A

A

V

C

L

V

V

A

T

T
x
S

Y
x
W

S

G

E

P

S

A

H
|
H

A

E

E

S

Y

S

A

V

I

L

A

K

A

A

M

I

E

K

Q

A

G

Q

A

K

D

Y

V

V

F

F

V

C

E
|
E

K
|
K

P

P

L

L

A

A

T

T

T

T

V

A

E

E

D

G

A

C

R

M

R

R

V

I

I

V

D

E

C

E

A

E

R

I

A

K

N

V

G

G

R

K

K

R

L

L

V

V

-

Q

I

V

G

G

Y

F

I
x
M

L

R

R

R

H

Y

H

D

P

S

S

G

W

Y

M

V

R

Q

L

L

I

K

E

E

-

A

-

L

E

D

A

N

R

H

K

V

L

I

G

G

G

E

P

P

Y

L

V

M

F

I

R

H

M

C

N

A

L

H

N

R

Q

N

Q

P

S

T

S

V

G

G

A

D

T

N

W

Y

E

-

T

-

H

-

K

-

N

-

L

-

M

-

N

T

T

T

T

D

S

M

P

A

I

V

V

V

D

D

C

T

G

L

V

V

H
|
H

Y

E

V

I

D

D

V

V

M

L

active site: K97 (= K98), H176 (= H181)
binding nadp nicotinamide-adenine-dinucleotide phosphate: K12 (≠ N18), I13 (≠ M19), S74 (≠ T75), W75 (≠ Y76), H79 (= H80), E96 (= E97), K97 (= K98), M126 (≠ I126)

Sites not aligning to the query:

binding nadp nicotinamide-adenine-dinucleotide phosphate: 272, 280

3nt5A Crystal structure of myo-inositol dehydrogenase from bacillus subtilis with bound cofactor and product inosose (see paper)
24% identity, 50% coverage: 9:186/356 of query aligns to 3:181/337 of 3nt5A

query
sites
3nt5A

V

L

R

R

V

I

L

G

S

V

A

I

G
|
G

L

T

G
|
G

N
x
A

M
x
I

G

G

R

K

S

E

H

H

A

I

-

N

L

R

A

I

Y

T

H

N

R

K

N

L

P

S

G

G

F

A

E

E

I

I

V

V

G

A

L

V

V

T

N
x
D

R
x
V

T

N

K

Q

V

E

P

A

V

A

P

Q

D

K

E

V

L

V

A

E

G

Q

Y

Y

-

Q

-

L

-

N

-

A

I

T

I

V

H

Y

P

P

S

N

F

D

H

D

E

S

A

L

L

L

K

A

E

D

L

E

K

N

P

V

D

D

L

A

A

V

C

L

V

V

A

T

T
x
S

Y
x
W

S
x
G

E

P

S

A

H

H

A

E

E

S

Y

S

A

V

I

L

A

K

A

A

M

I

E

K

Q

A

G

Q

A

K

D

Y

V

V

F

F

V

C

E
|
E

K
|
K

P

P

L

L

A

A

T

T

T

T

V

A

E

E

D

G

A

C

R

M

R

R

V

I

I

V

D

E

C

E

A

E

R

I

A

K

N

V

G

G

R

K

K

R

L

L

V

V

-

Q

I

V

G

G

Y

F

I

M

L

R

R

R

H

Y

H

D

P

S

S

G

W

Y

M

V

R

Q

L

L

I

K

E

E

-

A

-

L

E

D

A

N

R

H

K

V

L

I

G

G

G

E

P

P

Y

L

V

M

F

I

R

H

M

C

N

A

L
x
H

N

R

Q

N

Q

P

S

T

S

V

G

G

A

D

T

N

W

Y

E

-

T

-

H

-

K

-

N

-

L

-

M

-

N

T

T

T

T

D

S

M

P

A

I

V

V

V

D

D

C

T

G

L

V

V

H
|
H

Y

E

V

I

D

D

V

V

M

L

active site: K97 (= K98), H176 (= H181)
binding (2R,3S,4s,5R,6S)-2,3,4,5,6-pentahydroxycyclohexanone: K97 (= K98), H155 (≠ L153), H176 (= H181)
binding nicotinamide-adenine-dinucleotide: G9 (= G15), G11 (= G17), A12 (≠ N18), I13 (≠ M19), D35 (≠ N40), V36 (≠ R41), S74 (≠ T75), W75 (≠ Y76), G76 (≠ S77), E96 (= E97), K97 (= K98)

Sites not aligning to the query:

3nt4A Crystal structure of myo-inositol dehydrogenase from bacillus subtilis with bound cofactor nadh and inositol (see paper)
24% identity, 50% coverage: 9:186/356 of query aligns to 3:181/337 of 3nt4A

query
sites
3nt4A

V

L

R

R

V

I

L

G

S

V

A

I

G

G

L

T

G
|
G

N
x
A

M
x
I

G

G

R

K

S

E

H

H

A

I

-

N

L

R

A

I

Y

T

H

N

R

K

N

L

P

S

G

G

F

A

E

E

I

I

V

V

G

A

L

V

V

T

N
x
D

R
x
V

T

N

K

Q

V

E

P

A

V

A

P

Q

D

K

E

V

L

V

A

E

G

Q

Y

Y

-

Q

-

L

-

N

-

A

I

T

I

V

H

Y

P

P

S

N

F

D

H

D

E

S

A

L

L

L

K

A

E

D

L

E

K

N

P

V

D

D

L

A

A

V

C

L

V

V

A

T

T
x
S

Y
x
W

S
x
G

E

P

S

A

H

H

A

E

E

S

Y

S

A

V

I

L

A

K

A

A

M

I

E

K

Q

A

G

Q

A

K

D

Y

V

V

F

F

V

C

E
|
E

K
|
K

P

P

L

L

A

A

T

T

T

T

V

A

E

E

D

G

A

C

R

M

R

R

V

I

I

V

D

E

C

E

A

E

R

I

A

K

N

V

G

G

R

K

K

R

L

L

V

V

-

Q

I

V

G

G

Y

F

I

M

L

R

R

R

H

Y

H

D

P

S

S

G

W

Y

M

V

R

Q

L

L

I

K

E

E

-

A

-

L

E

D

A

N

R

H

K

V

L

I

G

G

G

E

P

P

Y

L

V

M

F

I

R

H

M

C

N

A

L
x
H

N

R

Q

N

Q

P

S

T

S

V

G

G

A

D

T

N

W

Y

E

-

T

-

H

-

K

-

N

-

L

-

M

-

N

T

T

T

T

D

S

M

P

A

I

V

V

V

D

D

C

T

G

L

V

V

H
|
H

Y

E

V

I

D

D

V

V

M

L

active site: K97 (= K98), H176 (= H181)
binding 1,2,3,4,5,6-hexahydroxy-cyclohexane: H155 (≠ L153), H176 (= H181)
binding 1,4-dihydronicotinamide adenine dinucleotide: G11 (= G17), A12 (≠ N18), I13 (≠ M19), D35 (≠ N40), V36 (≠ R41), S74 (≠ T75), W75 (≠ Y76), G76 (≠ S77), E96 (= E97), K97 (= K98), H176 (= H181)

Sites not aligning to the query:

binding 1,2,3,4,5,6-hexahydroxy-cyclohexane: 235, 272

3nt2B Crystal structure of myo-inositol dehydrogenase from bacillus subtilis with bound cofactor (see paper)
24% identity, 50% coverage: 9:186/356 of query aligns to 3:181/337 of 3nt2B

query
sites
3nt2B

V

L

R

R

V

I

L

G

S

V

A

I

G
|
G

L

T

G
|
G

N
x
A

M

I

G

G

R

K

S

E

H

H

A

I

-

N

L

R

A

I

Y

T

H

N

R

K

N

L

P

S

G

G

F

A

E

E

I

I

V

V

G

A

L

V

V

T

N
x
D

R
x
V

T

N

K

Q

V

E

P

A

V

A

P

Q

D

K

E

V

L

V

A

E

G

Q

Y

Y

-

Q

-

L

-

N

-

A

I

T

I

V

H

Y

P

P

S

N

F

D

H

D

E

S

A

L

L

L

K

A

E

D

L

E

K

N

P

V

D

D

L

A

A

V

C

L

V

V

A

T

T
x
S

Y
x
W

S

G

E

P

S
x
A

H

H

A

E

E

S

Y

S

A

V

I

L

A

K

A

A

M

I

E

K

Q

A

G

Q

A

K

D

Y

V

V

F

F

V

C

E

E

K
|
K

P

P

L

L

A

A

T

T

T

T

V

A

E

E

D

G

A

C

R

M

R

R

V

I

I

V

D

E

C

E

A

E

R

I

A

K

N

V

G

G

R

K

K

R

L

L

V

V

-

Q

I

V

G

G

Y

F

I

M

L

R

R

R

H

Y

H

D

P

S

S

G

W

Y

M

V

R

Q

L

L

I

K

E

E

-

A

-

L

E

D

A

N

R

H

K

V

L

I

G

G

G

E

P

P

Y

L

V

M

F

I

R

H

M

C

N

A

L

H

N

R

Q

N

Q

P

S

T

S

V

G

G

A

D

T

N

W

Y

E

-

T

-

H

-

K

-

N

-

L

-

M

-

N

T

T

T

T

D

S

M

P

A

I

V

V

V

D

D

C

T

G

L

V

V

H
|
H

Y

E

V

I

D

D

V

V

M

L

active site: K97 (= K98), H176 (= H181)
binding 1,4-dihydronicotinamide adenine dinucleotide: G9 (= G15), G11 (= G17), A12 (≠ N18), D35 (≠ N40), V36 (≠ R41), S74 (≠ T75), W75 (≠ Y76), A78 (≠ S79), K97 (= K98)

Sites not aligning to the query:

binding 1,4-dihydronicotinamide adenine dinucleotide: 272, 280

Query Sequence

>WP_004688980.1 NCBI__GCF_000022745.1:WP_004688980.1
MSSENQAPVRVLSAGLGNMGRSHALAYHRNPGFEIVGLVNRTKVPVPDELAGYIIHPSFH
EALKELKPDLACVATYSESHAEYAIAAMEQGADVFVEKPLATTVEDARRVIDCARANGRK
LVIGYILRHHPSWMRLIEEARKLGGPYVFRMNLNQQSSGATWETHKNLMNTTSPIVDCGV
HYVDVMCQITDARPVEVRGMGLRLSDEIKPDMYNYGHLQVLFDDGTVGWYEAGWGPMMSE
TAFFVKDVISPNGCVSIVMNEAVTKSDDVDAHTKTANIRIHRAERDADGRFLHPDTNLSM
ADEPGHQELCEREQAFMLKAIRENLDLDRHMNDALQSLRICLAADESVRSGQPVKL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory