SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_005344073.1 NCBI__GCF_000173975.1:WP_005344073.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P56459 Aspartate--tRNA(Asp/Asn) ligase; Aspartyl-tRNA synthetase; AspRS; Non-discriminating aspartyl-tRNA synthetase; ND-AspRS; EC 6.1.1.23 from Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) (see paper)
50% identity, 97% coverage: 10:591/597 of query aligns to 2:575/577 of P56459

query
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P56459

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L81 (≠ A91) mutation to N: Enhances enzyme specificity for tRNA(Asp) over tRNA(Asn), by reducing enzyme's ability to misacylate tRNA(Asn).
L86 (= L96) mutation to M: Enhances enzyme specificity for tRNA(Asp) over tRNA(Asn), by reducing enzyme's ability to misacylate tRNA(Asn).

Q51422 Aspartate--tRNA(Asp/Asn) ligase; Aspartyl-tRNA synthetase; AspRS; Non-discriminating aspartyl-tRNA synthetase; ND-AspRS; EC 6.1.1.23 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
48% identity, 99% coverage: 8:597/597 of query aligns to 1:591/591 of Q51422

query
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Q51422

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H31 (≠ L38) mutation to L: Enhances enzyme specificity for tRNA(Asp) over tRNA(Asn) by 3.5-fold, by reducing enzyme's ability to misacylate tRNA(Asn) when tested against E.coli tRNA, but shows little effect when tested against P.aeruginosa tRNA.
G82 (= G90) mutation to K: Enhances enzyme specificity for tRNA(Asp) over tRNA(Asn) by 4.2-fold, by reducing enzyme's ability to misacylate tRNA(Asn) when tested against E.coli tRNA, but shows little effect when tested against P.aeruginosa tRNA.

4wj3M Crystal structure of the asparagine transamidosome from pseudomonas aeruginosa (see paper)
48% identity, 97% coverage: 10:591/597 of query aligns to 2:583/589 of 4wj3M

query
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4wj3M

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A
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A

V

R

N
|
N

E

P

N

N

L

M

E

A

T

S

G

G

D

S

I

I

E
|
E

V

V

K

L

V

G

D

Y

S

E

I

L

R

E

I

V

L

L

S

N

E

Q

S

A

E

E

T
|
T

P

P

F

F

P

P

I

L

D
|
D

A

E

D

Y

I

S

T

D

V
|
V

K

G

D

E

E

E

L

T

R

R

L

L

K

R

Y

Y

R

R

F

F

L

I

D

D

L

L

R

R

P

P

N

E

I

M

Q

A

K

A

N

K

L

L

M

K

M

L

R

R

S

A

K

R

V

I

A

T

T

S

L

S

T

I

R

R

Q

R

F

Y

L

L

A

D

N

D

E

N

G

G

F

F

L

L

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D

I

V

E

E

T

T

P

P

M

I

L

L

T

G

R

R

S

P

T

T

P

P

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E

G

G

A

A

R

R

D

D

Y

Y

L

L

V

V

P

P

S

S

R

R

V

T

H

Y

P

P

G

G

S

H

F

F

Y

F

A

A

L

L

P

P

Q

Q

S

S

P

P

Q

Q

L

L

F

F

K

K

Q

Q

L

L

M

M

C

V

S

A

G

G

Y

F

D

D

R

R

Y

Y

F

Y

Q

Q

L

I

A

A

R

K

C

C

F

F

R
|
R

D

D

E
|
E

D

D

L

L

R

R

A

A

D

D

R
|
R

Q
|
Q

P

P

E

E

F

F

T

T

Q

Q

I

I

D

D

M

I

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E

L

T

S

S

F

F

V

L

D

D

V

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D

S

D

D

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I

I

I

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G

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I

N

T

E

E

R

K

L

M

L

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A

R

Y

Q

L

L

F

F

K

K

L

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C

V

L

L

N

D

E

V

E

E

V

F

S

D

L

-

P

E

I

F

Q

P

R

H

M

M

T

P

W

F

Q

E

D

E

A

A

M

M

D

R

Y

R

Y

Y

G

G

S

S

D

D

K

K

P

P

D

D

L

L

R

R

F

I

D

P

M

L

K

E

I

L

Q

V

D

D

V

V

S

A

E

D

L

Q

V

L

K

K

D

E

C

V

G

E

F

F

G

K

V

V

F

F

T

S

G

G

-

P

A

A

I

N

E

D

N

P

G

K

G

G

M

R

V

V

R

A

G

A

L

L

C

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A

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G

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M

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P

S

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K

S

K

Q

M

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K

D

H

D

I

Y

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T

K

K

T

F

A

V

K

G

D

I

Y

Y

G

G

A

A

K

K

G

G

L

L

A

A

Y

Y

I

I

Q

K

L

V

-

N

-

E

K

R

S

A

D

K

G

G

T

V

-

E

-

G

V

L

K

Q

C

S

P

P

F

I

S

V

K

K

F

F

M

I

S

P

E

E

E

A

E

N

L

L

Q

N

A

V

L

I

I

L

Q

D

A

R

M

V

G

G

G

A

E

V

T

D

G

G

D

D

L

I

L

V

V

F

F

F

A

G

A

A

D

D

K

K

F

A

K

K

V

I

V

V

C

C

D

D

V

A

L

L

G

G

A

A

L

L

R

R

L

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K

K

F

V

A

G

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H

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D

L

L

N

K

L

L

D

T

K

R

S

-

E

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Y

W

R

A

F

P

V

M

W

W

I

V

T

V

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D

F

F

P

P

L

M

L

F

E

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W

-

S

E

E

N

E

D

N

G

R

S

Y

L

T

S

A

A

M

L

H

H

P

P

F

F

T

T

M

S

P

P

M

K

E

C

E

T

D

P

L

A

D

E

M

L

I

-

D

E

T

A

E

N

P

P

G

G

K

A

V

A

R

L

A

S

K

R

A

A

Y

Y

D

D

I

M

V

V

L

L

N

N

G

G

T

T

E
|
E

I

L

G

G

G
|
G

G

G

S

S

V

I

R

R

I

I

H

H

Q

D

N

K

D

S

I

M

Q

Q

E

Q

K

A

M

V

F

F

E

R

C

V

L

L

G

G

I

I

S

D

K

E

E

A

Q

E

A

Q

Q

E

E

E

K

K

F

F

G

G

F

F

L

L

E

D

A

A

F

L

K

K

Y

Y

G

G

V

A

P

P

H

H

A

G

G

G

L

L

A

A

Y

F

G

G

L

L

D

D

R
|
R

L

L

V

V

M

M

L

L

M

M

T

T

K

G

E

A

D

S

T

S

I

I

R

R

D

E

V

V

I

I

A

A

F

F

P

P

K

K

V

T

K

Q

D

S

A

A

S

G

C

D

L

V

L

M

T

T

D

Q

A

A

P

P

N

G

T

S

V

V

D

D

D

G

K

K

Q

A

L

L

E

R

E

E

L

L

C

H

L

I

E

R

I

L

active site: R219 (= R228), E221 (= E230), R227 (= R236), Q228 (= Q237), E482 (= E490), G485 (= G493), R537 (= R545)
binding : R28 (= R36), D29 (≠ N37), H30 (≠ L38), G32 (≠ S40), V33 (≠ L41), F35 (= F43), Q46 (= Q54), R64 (= R74), R76 (≠ E86), R78 (= R88), A82 (= A91), N84 (= N93), E93 (= E102), T107 (= T116), D113 (= D122), V118 (= V127)

4wj4A Crystal structure of non-discriminating aspartyl-tRNA synthetase from pseudomonas aeruginosa complexed with tRNA(asn) and aspartic acid (see paper)
48% identity, 97% coverage: 10:591/597 of query aligns to 2:583/585 of 4wj4A

query
sites
4wj4A

R

R

S

S

H

H

R

Y

C

C

T

G

E

Q

V

L

S

N

N

E

E

S

N

L

I

D

G

G

E

Q

T

E

V

V

T

T

L

L

M

C

G

G

W

W

V

V

Q

H

K
x
R

R

R

R
|
R

N
x
D

L
x
H

G
|
G

S
x
G

L
x
V

I

I

F
|
F

V

L

D

D

L

V

R

R

D

D

R

R

S

E

G

G

L

L

I

A

Q
|
Q

L

V

Y

V

F

F

D

D

E

P

E

D

T

R

I

-

G

-

E

A

E

E

G

T

F

F

K

A

K

K

A

A

A

D

S

R

L

V

R
|
R

A

S

E

E

F

F

V

V

I

V

A

K

V

I

T

T

G

G

T

K

V

V

E
x
R

K

L

R

R

S
x
P

-

E

G

G

A
|
A

V

R

N
|
N

E

P

N

N

L

M

E

A

T

S

G

G

D

S

I

I

E
|
E

V

V

K

L

V

G

D

Y

S

E

I

L

R

E

I

V

L

L

S

N

E

Q

S

A

E

E

T
|
T

P

P

P
|
P

F

F

P

P

I

L

D
|
D

A
x
E

D

Y

I

S

T
x
D

V

V

K

G

D

E

E
|
E

L

T

R

R

L

L

K

R

Y

Y

R

R

F

F

L

I

D

D

L

L

R

R

P

P

N

E

I

M

Q

A

K

A

N

K

L

L

M

K

M

L

R

R

S

A

K

R

V

I

A

T

T

S

L

S

T

I

R

R

Q

R

F

Y

L

L

A

D

N

D

E

N

G

G

F

F

L

L

E

D

I

V

E

E

T

T

P

P

M

I

L

L

T

G

R

R

S

P

T

T

P

P

E

E

G

G

A
|
A

R

R

D

D

Y

Y

L

L

V

V

P

P

S

S

R

R

V

T

H

Y

P

P

G

G

S

H

F

F

Y

F

A

A

L

L

P

P

Q

Q

S
|
S

P

P

Q
|
Q

L

L

F

F

K

K

Q

Q

L

L

M

M

C

V

S

A

G

G

Y

F

D

D

R

R

Y

Y

F

Y

Q

Q

L

I

A

A

R

K

C

C

F

F

R
|
R

D

D

E
|
E

D
|
D

L

L

R
|
R

A
|
A

D

D

R
|
R

Q
|
Q

P

P

E

E

F

F

T

T

Q

Q

I

I

D

D

M

I

E

E

L

T

S

S

F

F

V

L

D

D

V

E

D

S

D

D

V

I

I

I

D

G

V

I

N

T

E

E

R

K

L

M

L

V

A

R

Y

Q

L

L

F

F

K

K

L

E

C

V

L

L

N

D

E

V

E

E

V

F

S

D

L

-

P

E

I

F

Q

P

R

H

M

M

T

P

W

F

Q

E

D

E

A

A

M

M

D

R

Y

R

Y

Y

G

G

S

S

D

D

K

K

P

P

D

D

L

L

R

R

F

I

D

P

M

L

K

E

I

L

Q

V

D

D

V

V

S

A

E

D

L

Q

V

L

K

K

D

E

C

V

G

E

F

F

G

K

V

V

F

F

T

S

G

G

-

P

A

A

I

N

E

D

N

P

G

K

G

G

M

R

V

V

R

A

G

A

L

L

C

R

A

V

K

P

G

G

L

A

G

A

D

S

V

M

S

P

S

R

K

S

K

Q

M

I

K

D

H

D

I

Y

E

T

K

K

T

F

A

V

K

G

D

I

Y
|
Y

G

G

A

A

K

K

G

G

L

L

A

A

Y

Y

I

I

Q

K

L

V

-

N

-

E

K

R

S

A

D

K

G

G

T

V

-

E

-

G

V

L

K

Q

C

S

P

P

F

I

S

V

K

K

F

F

M

I

S

P

E

E

E

A

E

N

L

L

Q

N

A

V

L

I

I

L

Q

D

A

R

M

V

G

G

G

A

E

V

T

D

G

G

D

D

L

I

L

V

V

F

F

F

A

G

A

A

D

D

K

K

F

A

K

K

V

I

V

V

C

C

D

D

V

A

L

L

G

G

A

A

L

L

R

R

L

I

K

K

F

V

A

G

E

H

E

D

L

L

N

K

L

L

D

T

K

R

S

-

E

E

Y

W

R

A

F

P

V

M

W

W

I

V

T

V

E

D

F

F

P

P

L

M

L

F

E

E

W

-

S

E

E

N

E

D

N

G

R

S

Y

L

T

S

A
|
A

M

L

H
|
H

P

P

F

F

T

T

M

S

P

P

M

K

E

C

E

T

D

P

L

A

D

E

M

L

I

-

D

E

T

A

E

N

P

P

G

G

K

A

V

A

R

L

A

S

K

R

A

A

Y

Y

D

D

I

M

V

V

L

L

N

N

G

G

T

T

E
|
E

I

L

G

G

G
|
G

G

G

S

S

V

I

R
|
R

I

I

H

H

Q

D

N

K

D

S

I

M

Q

Q

E

Q

K

A

M

V

F

F

E

R

C

V

L

L

G

G

I

I

S

D

K

E

E

A

Q

E

A

Q

Q

E

E

E

K

K

F

F

G

G

F

F

L

L

E

D

A

A

F

L

K

K

Y

Y

G

G

V

A

P

P

H

H

A

G

G

G

L
|
L

A

A

Y

F

G

G

L

L

D

D

R
|
R

L

L

V

V

M

M

L

L

M

M

T

T

K

G

E

A

D

S

T

S

I

I

R
|
R

D

E

V

V

I

I

A

A

F

F

P

P

K

K

V
x
T

K
x
Q

D
x
S

A

A

S

G

C

D

L

V

L

M

T

T

D

Q

A

A

P

P

N

G

T

S

V

V

D

D

D

G

K

K

Q

A

L

L

E

R

E

E

L

L

C

H

L

I

E

R

I

L

active site: R219 (= R228), E221 (= E230), R227 (= R236), Q228 (= Q237), E482 (= E490), G485 (= G493), R537 (= R545)
binding aspartic acid: S195 (= S204), Q197 (= Q206), H450 (= H457), R489 (= R497), L531 (= L539)
binding : R26 (≠ K34), R28 (= R36), D29 (≠ N37), H30 (≠ L38), G31 (= G39), G32 (≠ S40), V33 (≠ L41), F35 (= F43), Q46 (= Q54), R64 (= R74), R76 (≠ E86), P79 (≠ S89), A82 (= A91), N84 (= N93), E93 (= E102), T107 (= T116), P109 (= P118), D113 (= D122), E114 (≠ A123), D117 (≠ T126), E121 (= E130), A175 (= A184), E221 (= E230), D222 (= D231), R224 (= R233), A225 (= A234), R227 (= R236), Y346 (= Y355), A447 (= A454), H449 (= H456), H450 (= H457), R549 (= R557), T557 (≠ V565), Q558 (≠ K566), S559 (≠ D567)

1c0aA Crystal structure of the e. Coli aspartyl-tRNA synthetase : trnaasp : aspartyl-adenylate complex (see paper)
48% identity, 97% coverage: 10:591/597 of query aligns to 2:583/585 of 1c0aA

query
sites
1c0aA

R

R

S

T

H

E

R

Y

C

C

T

G

E

Q

V

L

S

R

N

L

E

S

N

H

I

V

G

G

E

Q

T

Q

V

V

T

T

L

L

M

C

G

G

W

W

V

V

Q

N

K
x
R

R

R

R
|
R

N
x
D

L
|
L

G
|
G

S
|
S

L
|
L

I

I

F
|
F

V

I

D

D

L

M

R

R

D

D

R

R

S

E

G

G

L

I

I

V

Q
|
Q

L

V

Y
x
F

F

F

D
|
D

E
x
P

E

D

T

R

I

-

G

-

E

A

E

D

G

A

F

L

K

K

K

L

A

A

A

S

S

E

L

L

R
|
R

A

N

E

E

F

F

V

C

I

I

A

Q

V

V

T

T

G

G

T

T

V

V

E
x
R

K

A

R
|
R

S

D

-

E

G

K

A
x
N

V

I

N
|
N

E

R

N

D

L
x
M

E

A

T

T

G

G

D

E

I

I

E
|
E

V

V

K

L

V

A

D

S

S

S

I

L

R

T

I

I

L

I

S

N

E

R

S

A

E

D

T

V

P

-

F

L

P
|
P

I

L

D
|
D

A

S

D
x
N

I
x
H

T

V

V
x
N

K
x
T

D

E

E
|
E

L

A

R

R

L

L

K

K

Y

Y

R

R

F

Y

L

L

D

D

L

L

R

R

P

P

N

E

I

M

Q

A

K

Q

N

R

L

L

M

K

M

T

R

R

S

A

K

K

V

I

A

T

T

S

L

L

T

V

R

R

Q

R

F

F

L

M

A

D

N

D

E

H

G

G

F

F

L

L

E

D

I

I

E

E

T

T

P

P

M

M

L

L

T

T

R

K

S

A

T
|
T

P
|
P

E
|
E

G
|
G

A
|
A

R

R

D

D

Y

Y

L

L

V

V

P

P

S

S

R

R

V

V

H

H

P

K

G

G

S

K

F

F

Y

Y

A

A

L

L

P

P

Q

Q

S
|
S

P

P

Q
|
Q

L

L

F

F

K
|
K

Q

Q

L

L

M

M

C

M

S

S

G

G

Y

F

D

D

R

R

Y

Y

F

Y

Q

Q

L

I

A

V

R

K

C

C

F

F

R
|
R

D

D

E
|
E

D
|
D

L

L

R
|
R

A
|
A

D

D

R
|
R

Q
|
Q

P

P

E

E

F
|
F

T

T

Q
|
Q

I

I

D

D

M

V

E

E

L

T

S

S

F

F

V

M

D

T

V

A

D

P

D

Q

V

V

I

R

D

E

V

V

N

M

E

E

R

A

L

L

L

V

A

R

Y

H

L

L

F

W

K

L

L

E

C

V

L

K

N

G

E

V

E

D

V

L

S

G

-

D

L

F

P

P

I

V

Q

-

R

-

M

M

T

T

W

F

Q

A

D

E

A

A

M

E

D

R

Y

R

Y

Y

G

G

S

S

D

D

K

K

P

P

D

D

L

L

R

R

F

N

D

P

M

M

K

E

I

L

Q

T

D

D

V

V

S

A

E

D

L

L

V

L

K

K

D

S

C

V

G

E

F
|
F

G

A

V
|
V

F

F

T

A

G

G

-

P

A

A

I

N

E

D

N

P

G

K

G

G

M

R

V

V

R

A

G

A

L

L

C

R

A

V

K

P

G

G

L

G

G

A

D

S

V

L

S

T

S

R

K

K

K

Q

M

I

K

D

H

E

I

Y

E

G

K

N

T

F

A

V

K

K

D
x
I

Y

Y

G

G

A

A

K
|
K

G
|
G

L

L

A
|
A

Y

Y

I

I

Q

K

L

V

K

N

S

E

D

R

G

A

T

K

-

G

-

L

-

E

-

G

V

I

K

N

C

S

P

P

F

V

S

A

K

K

F

F

M

L

S

N

E

A

E

E

E

I

L

I

Q

E

A

D

L

I

I

L

Q

D

A

R

M

T

G

A

E

Q

T

D

G

G

D

D

L

M

L

I

V

F

F

F

A

G

A

A

D

D

K

N

F

K

K

K

V

I

V

V

C

A

D

D

V

A

L

M

G

G

A

A

L

L

R

R

L

L

K

K

F

V

A

G

E

K

E

D

L

L

N

G

L

L

L

T

D

D

K

E

S

S

E

K

Y

W

R

A

F

P

V

L

W

W

I

V

T

I

E

D

F

F

P

P

L

M

L

F

E

E

W

-

S

D

E

D

E

G

E

E

N

G

R

G

Y

L

T

T

A

A

M

M

H
|
H

P

P

F

F

T

T

M

S

P

P

M

K

E

D

E

M

D

T

L

A

D

A

M

E

I

L

D

K

T

A

E

A

P

P

G

E

K

N

V

A

R

V

A

A

K

N

A

A

Y

Y

D

D

I

M

V

V

L

I

N

N

G

G

T

Y

E
|
E

I
x
V

G
|
G

S

S

V

V

R
|
R

I

I

H

H

Q

N

N

G

D

D

I

M

Q

Q

E

Q

K

T

M

V

F

F

E

G

C

I

L

L

G

G

I

I

S

N

K

E

E

E

Q

E

A

Q

Q

R

E

E

K

K

F
|
F

G

G

F

F

L

L

E

D

A

A

F

L

K

K

Y

Y

G

G

V

T

P

P

H

H

A

A

G

G

L
|
L

A
|
A

Y

F

G
|
G

L

L

D

D

R
|
R

L

L

V

T

M

M

L

L

M

L

T

T

K

G

E

T

D

D

T

N

I

I

R
|
R

D

D

V

V

I

I

A

A

F

F

P

P

K

K

V
x
T

K
x
T

D
x
A

A

A

S

A

C

C

L

L

L

M

T

T

D

E

A

A

P

P

N

S

T

F

V

A

D

N

D

P

K

T

Q

A

L

L

E

A

E

E

L

L

C

S

L

I

E

Q

I

V

active site: E482 (= E490), G485 (= G493), R537 (= R545)
binding aspartyl-adenosine-5'-monophosphate: S193 (= S204), Q195 (= Q206), K198 (= K209), R217 (= R228), Q226 (= Q237), F229 (= F240), Q231 (= Q242), H448 (= H456), E482 (= E490), V483 (≠ I491), G484 (= G492), G485 (= G493), G486 (= G494), R489 (= R497), L531 (= L539), A532 (= A540), G534 (= G542), R537 (= R545)
binding adenosine monophosphate: F304 (= F316), V306 (= V318), K347 (= K358), G348 (= G359), A350 (= A361)
binding : R26 (≠ K34), R28 (= R36), D29 (≠ N37), L30 (= L38), G31 (= G39), S32 (= S40), L33 (= L41), F35 (= F43), Q46 (= Q54), F48 (≠ Y56), D50 (= D58), P51 (≠ E59), R64 (= R74), R76 (≠ E86), R78 (= R88), N82 (≠ A91), N84 (= N93), M87 (≠ L96), E93 (= E102), P109 (= P120), D111 (= D122), N113 (≠ D124), H114 (≠ I125), N116 (≠ V127), T117 (≠ K128), E119 (= E130), T169 (= T180), P170 (= P181), E171 (= E182), G172 (= G183), A173 (= A184), S193 (= S204), R217 (= R228), E219 (= E230), D220 (= D231), R222 (= R233), A223 (= A234), R225 (= R236), I343 (≠ D354), H448 (= H456), H449 (= H457), F514 (= F522), R549 (= R557), T557 (≠ V565), T558 (≠ K566), A559 (≠ D567)

6sjcB Structure of t. Thermophilus asprs in complex with 5'-o-(n-(l- aspartyl)-sulfamoyl)adenosine (see paper)
49% identity, 98% coverage: 7:594/597 of query aligns to 1:581/581 of 6sjcB

query
sites
6sjcB

G

G

L

M

K

R

R

R

S

T

H

H

R

Y

C

A

T

G

E

S

V

L

S

R

N

E

E

T

N

H

I

V

G

G

E

E

T

E

V

V

T

V

L

L

M

E

G

G

W

W

V

V

Q

N

K

R

R

R

N

D

L

L

G

G

S

G

L

L

I

I

F

F

V

L

D

D

L

L

R

R

D

D

R

R

S

E

G

G

L

L

I

V

Q

Q

L

L

Y

V

F

A

D

H

E

P

E

A

T

S

I

-

G

-

E

-

E

P

G

A

F

Y

K

A

K

T

A

A

A

E

S

R

L

V

R

R

A

P

E

E

F

W

V

V

I

V

A

R

V

A

T

K

G

G

T

L

V

V

E

R

K

L

R

R

S

P

G

-

A

E

V

P

N

N

E

P

N

R

L

L

E

A

T

T

G

G

D

R

I

V

E

E

V

V

K

E

V

L

D

S

S

A

I

L

R

E

I

V

L

L

S

A

E

E

S

A

E

K

T

T

P

P

F

F

P

P

I

V

D

D

A

A

D

G

-

W

-

R

-

G

-

E

I

K

T

E

V

A

K

S

D

E

E

E

L

L

R

R

L

L

K

K

Y

Y

R

R

F

Y

L

L

D

D

L

L

R

R

P

R

N

R

I

M

Q

Q

K

E

N

N

L

L

M

R

M

L

R

R

S

H

K

R

V

V

A

I

T

K

L

A

T

I

R

W

Q

D

F

F

L

L

A

D

N

R

E

E

G

G

F

F

L

V

E

Q

I

V

E

E

T

T

P

P

M

F

L

L

T

T

R

K

S

S

T

T

P

P

E
|
E

G

G

A

A

R

R

D

D

Y

F

L

L

V

V

P

P

S

Y

R

R

V

H

H

E

P

P

G

G

S

L

F

F

Y

Y

A

A

L

L

P

P

Q

Q

S

S

P

P

Q
|
Q

L

L

F

F

K
|
K

Q

Q

L

M

L

L

M

M

C

V

S

A

G

G

Y

L

D

D

R

R

Y

Y

F

F

Q

Q

L

I

A

A

R

R

C

C

F

F

R
|
R

D

D

E

E

D

D

L

L

R

R

A

A

D

D

R
|
R

Q
|
Q

P

P

E

D

F
|
F

T

T

Q
|
Q

I

L

D

D

M

L

E

E

L

M

S

S

F

F

V

V

D

E

V

V

D

E

D

D

V

V

I

L

D

E

V

L

N

N

E

E

R

R

L

L

L

M

A

A

Y

H

L

V

F

F

K

R

L

E

C

A

L

L

N

G

E

V

E

E

V

L

S

P

L

L

P

P

I

F

Q

P

R

R

M

L

T

S

W

Y

Q

E

D

E

A

A

M

M

D

E

Y

R

Y

Y

G

G

S

S

D

D

K

K

P

P

D

D

L

L

R

R

F

F

D

G

M

L

K

E

I

L

Q

K

D

E

V

V

S

G

E

P

L

L

V

F

K

R

D

Q

C

S

G

G

F

F

G

R

V

V

F

F

T

-

G

-

A

-

I

-

E

Q

N

E

G

A

G

E

M

S

V

V

R

K

G

A

L

L

C

A

-

L

A

P

K

K

G

A

L

L

G

-

D

-

V

-

S

S

S

R

K

K

K

E

M

V

K

A

H

E

I

L

E

E

K

E

T

V

A

A

K

K

D

R

Y

H

G

K

A

A

K

Q

G

G

L

L

A

A

Y

W

I

A

Q

R

L

V

K

E

S

-

D

E

G

G

T

G

V

F

K

S

C

G

P

G

F

V

S

A

K

K

F

F

M

L

S

-

E

E

E

P

E

V

L

R

Q

E

A

A

L

L

I

L

Q

Q

A

A

M

T

G

E

G

A

E

R

T

P

G

G

D

D

L

T

L

L

V

L

F

F

A

V

A

A

D

G

K

P

F

R

K

K

V

V

V

A

C

A

D

T

V

A

L

L

G

G

A

A

L

V

R

R

L

L

K

R

F

A

A

A

E

D

E

L

L

L

N

G

L

-

L

L

D

K

K

R

S

E

E

G

Y

F

R

R

F

F

V

L

W

W

I

V

T

V

E

D

F

F

P

P

L

L

E

E

W

W

S

D

E

E

N

E

R

A

Y

W

T

T

A

Y

M

M

H

H

P

P

F

F

T

T

M

S

P

P

M

H

E

P

E

E

D

D

L

L

D

P

M

L

I

L

D

E

T

K

E

D

P

P

G

G

K

R

V

V

R

R

A

A

K

L

A

A

Y

Y

D

D

I

L

V

V

L

L

N

N

G

G

T

V

E
|
E

I
x
V

G
|
G

S

S

V

I

R
|
R

I

I

H

H

Q

D

N

P

D

R

I

L

Q

Q

E

A

K

R

M

V

F

F

E

R

C

L

L

L

G

G

I

I

S

G

K

E

E

E

Q

E

A

Q

Q

R

E

E

K

K

F

F

G

G

F

F

L

F

L

L

E

E

A

A

F

L

K

E

Y

Y

G

G

V

A

P

P

H

H

A

G

G

G

L
x
I

A
|
A

Y

W

G
|
G

L

L

D

D

R
|
R

L

L

V

L

M

A

L

L

M

M

T

T

K

G

E

S

D

P

T

S

I

I

R

R

D

E

V

V

I

I

A

A

F

F

P

P

K

K

V

N

K

K

D

E

A

G

S

K

C

D

L

P

L

L

T

T

D

G

A

A

P

P

N

S

T

P

V

V

D

P

D

E

K

E

Q

Q

L

L

E

R

E

E

L

L

C

G

L

L

E

M

I

V

E

V

I

R

P

P

binding 5'-O-(L-alpha-aspartylsulfamoyl)adenosine: E178 (= E182), Q202 (= Q206), K205 (= K209), R224 (= R228), R232 (= R236), Q233 (= Q237), F236 (= F240), Q238 (= Q242), E477 (= E490), V478 (≠ I491), G479 (= G492), G480 (= G493), G481 (= G494), R484 (= R497), I526 (≠ L539), A527 (= A540), G529 (= G542), R532 (= R545)

1g51B Aspartyl tRNA synthetase from thermus thermophilus at 2.4 a resolution (see paper)
49% identity, 98% coverage: 8:594/597 of query aligns to 1:580/580 of 1g51B

query
sites
1g51B

L

M

K

R

R

R

S

T

H

H

R

Y

C

A

T

G

E

S

V

L

S

R

N

E

E

T

N

H

I

V

G

G

E

E

T

E

V

V

T

V

L

L

M

E

G

G

W

W

V

V

Q

N

K

R

R

R

N

D

L

L

G

G

S

G

L

L

I

I

F

F

V

L

D

D

L

L

R

R

D

D

R

R

S

E

G

G

L

L

I

V

Q

Q

L

L

Y

V

F

A

D

H

E

P

E

A

T

S

I

-

G

-

E

-

E

P

G

A

F

Y

K

A

K

T

A

A

A

E

S

R

L

V

R

R

A

P

E

E

F

W

V

V

I

V

A

R

V

A

T

K

G

G

T

L

V

V

E

R

K

L

R

R

S

P

G

E

A

P

V

-

N

N

E

P

N

R

L

L

E

A

T

T

G

G

D

R

I

V

E

E

V

V

K

E

V

L

D

S

S

A

I

L

R

E

I

V

L

L

S

A

E

E

S

A

E

K

T

T

P

P

F

F

P

P

I

V

D

D

A

A

D

G

-

W

-

R

-

G

-

E

I

K

T

E

V

A

K

S

D

E

E

E

L

L

R

R

L

L

K

K

Y

Y

R

R

F

Y

L

L

D

D

L

L

R

R

P

R

N

R

I

M

Q

Q

K

E

N

N

L

L

M

R

M

L

R

R

S

H

K

R

V

V

A

I

T

K

L

A

T

I

R

W

Q

D

F

F

L

L

A

D

N

R

E

E

G

G

F

F

L

V

E

Q

I

V

E

E

T

T

P

P

M

F

L

L

T

T

R

K

S

S

T

T

P

P

E
|
E

G

G

A

A

R

R

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D

Y

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L

L

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V

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P

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Y

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H

H

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P

P

G

G

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L

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F

Y

Y

A

A

L

L

P

P

Q

Q

S
|
S

P

P

Q
|
Q

L

L

F

F

K
|
K

Q

Q

L

M

L

L

M

M

C

V

S

A

G

G

Y

L

D

D

R

R

Y

Y

F

F

Q

Q

L

I

A

A

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C

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R

D

D

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E

D

D

L

L

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D

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R

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Q

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F

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Q

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L

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D

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D

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K

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P

D

D

L

L

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K

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L

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G

G

F

F

G

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|
V

F

F

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-

G

-

A

-

I

-

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Q

N

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A

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A

-

L

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A

A

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D

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Y

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G

K

A

A

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x
Q

G
|
G

L
|
L

A
|
A

Y

W

I

A

Q

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L

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-

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x
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|
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|
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|
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|
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I

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|
A

Y

W

G
|
G

L

L

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D

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|
R

L

L

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L

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A

L

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K

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K

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L

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G

L

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I

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E

V

I

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active site: R223 (= R228), E225 (= E230), R231 (= R236), Q232 (= Q237), E476 (= E490), G479 (= G493), R531 (= R545)
binding aspartyl-adenosine-5'-monophosphate: E177 (= E182), S199 (= S204), Q201 (= Q206), K204 (= K209), R223 (= R228), Q232 (= Q237), F235 (= F240), Q237 (= Q242), H442 (= H456), E476 (= E490), G478 (= G492), G479 (= G493), G480 (= G494), R483 (= R497), I525 (≠ L539), A526 (= A540), G528 (= G542), R531 (= R545)
binding adenosine monophosphate: V313 (= V318), Q347 (≠ K358), G348 (= G359), L349 (= L360), A350 (= A361), V389 (≠ A402), A390 (= A403)

1g51A Aspartyl tRNA synthetase from thermus thermophilus at 2.4 a resolution (see paper)
49% identity, 98% coverage: 8:594/597 of query aligns to 1:580/580 of 1g51A

query
sites
1g51A

L

M

K

R

R

R

S

T

H

H

R

Y

C

A

T

G

E

S

V

L

S

R

N

E

E

T

N

H

I

V

G

G

E

E

T

E

V

V

T

V

L

L

M

E

G

G

W

W

V

V

Q

N

K

R

R

R

N

D

L

L

G

G

S

G

L

L

I

I

F

F

V

L

D

D

L

L

R

R

D

D

R

R

S

E

G

G

L

L

I

V

Q

Q

L

L

Y

V

F

A

D

H

E

P

E

A

T

S

I

-

G

-

E

-

E

P

G

A

F

Y

K

A

K

T

A

A

A

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S

R

L

V

R

R

A

P

E

E

F

W

V

V

I

V

A

R

V

A

T

K

G

G

T

L

V

V

E

R

K

L

R

R

S

P

G

E

A

P

V

-

N

N

E

P

N

R

L

L

E

A

T

T

G

G

D

R

I

V

E

E

V

V

K

E

V

L

D

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S

A

I

L

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I

V

L

L

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A

E

E

S

A

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K

T

T

P

P

F

F

P

P

I

V

D

D

A

A

D

G

-

W

-

R

-

G

-

E

I

K

T

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A

K

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D

E

E

E

L

L

R

R

L

L

K

K

Y

Y

R

R

F

Y

L

L

D

D

L

L

R

R

P

R

N

R

I

M

Q

Q

K

E

N

N

L

L

M

R

M

L

R

R

S

H

K

R

V

V

A

I

T

K

L

A

T

I

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W

Q

D

F

F

L

L

A

D

N

R

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E

G

G

F

F

L

V

E

Q

I

V

E

E

T

T

P

P

M

F

L

L

T

T

R

K

S

S

T

T

P

P

E
|
E

G

G

A

A

R

R

D

D

Y

F

L

L

V

V

P

P

S

Y

R

R

V

H

H

E

P

P

G

G

S

L

F

F

Y

Y

A

A

L

L

P

P

Q

Q

S

S

P

P

Q
|
Q

L

L

F

F

K
|
K

Q

Q

L

M

L

L

M

M

C

V

S

A

G

G

Y

L

D

D

R

R

Y

Y

F

F

Q

Q

L

I

A

A

R

R

C

C

F

F

R
|
R

D

D

E
|
E

D

D

L

L

R

R

A

A

D

D

R
|
R

Q
|
Q

P

P

E

D

F
|
F

T

T

Q
|
Q

I

L

D

D

M

L

E

E

L

M

S

S

F

F

V

V

D

E