SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_005345775.1 NCBI__GCF_000173975.1:WP_005345775.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

1gdeA Crystal structure of pyrococcus protein a-1 e-form (see paper)
47% identity, 99% coverage: 5:391/391 of query aligns to 2:387/388 of 1gdeA

query
sites
1gdeA

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active site: K232 (= K234)
binding glutamic acid: F120 (= F123), N170 (= N173), R361 (= R361)
binding pyridoxal-5'-phosphate: G94 (= G97), A95 (≠ G98), N96 (≠ S99), F120 (= F123), N166 (≠ S169), D198 (= D201), Y201 (= Y204), S231 (= S233), K232 (= K234), R240 (= R242)

1gd9A Crystall structure of pyrococcus protein-a1 (see paper)
47% identity, 99% coverage: 5:391/391 of query aligns to 2:387/388 of 1gd9A

query
sites
1gd9A

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active site: K232 (= K234)
binding pyridoxal-5'-phosphate: G94 (= G97), A95 (≠ G98), N96 (≠ S99), F120 (= F123), N170 (= N173), D198 (= D201), V200 (≠ I203), Y201 (= Y204), S231 (= S233), K232 (= K234), R240 (= R242)

1j32A Aspartate aminotransferase from phormidium lapideum
42% identity, 91% coverage: 29:383/391 of query aligns to 31:385/388 of 1j32A

query
sites
1j32A

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active site: W124 (≠ F123), D202 (= D201), I204 (= I203), K237 (= K234)
binding pyridoxal-5'-phosphate: G98 (= G97), G99 (= G98), K100 (≠ S99), W124 (≠ F123), N174 (= N173), D202 (= D201), I204 (= I203), Y205 (= Y204), K237 (= K234), R245 (= R242)

5wmiA Arabidopsis thaliana prephenate aminotransferase mutant- t84v (see paper)
40% identity, 98% coverage: 5:386/391 of query aligns to 3:400/402 of 5wmiA

query
sites
5wmiA

L

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V

T

S

V

N

G

G

G

A

S

K

E

Q

G

S

I

L

D

L

L

Q

A

A

L

V

R

L

A

A

M

V

L

C

N

S

P

P

G

G

D

D

E

E

V

V

I

I

L

I

P

P

E

A

P

P

A

Y

F

W

V

V

S

S

Y

Y

L

T

P

E

C

Q

V

A

K

R

L

L

A

A

D

D

G

A

V

T

P

P

V

V

T

V

I

I

D

P

L

T

K

K

E

I

E

S

N

N

H

N

F

F

K

L

L

L

K

D

P

P

E

K

E

D

L

L

L

E

A

S

V

K

I

L

T

T

D

E

K

K

T

S

K

R

I

L

L

L

I

I

L

L

S

C

Y

S

P

P

N

S

N
|
N

P

P

T

T

G

G

A

S

I

V

M

Y

T

P

R

K

E

S

D

L

L

L

E

E

P

E

I

I

A

A

E

R

I

I

V

I

-

A

K

K

E

H

K

P

D

R

L

L

Y

L

V

V

I

L

S

S

D

D

E

E

I

I

Y

Y

A

E

E

H

L

I

T

I

Y

Y

G

A

-

P

Q

A

D

T

H

H

C

T

S

S

I

F

A

A

S

S

L

L

P

P

G

D

M

M

R

Y

D

E

R

R

T

T

I

L

I

T

I

V

N

N

G

G

F

F

S

S

K
|
K

S

A

F

F

A

A

M

M

T

T

G

G

W

W

R

R

M

L

G

G

F

Y

A

L

T

A

G

G

P

P

E

K

L

H

I

I

M

V

Q

A

Q

A

I

C

L

S

K

K

I

L

H

Q

Q

G

F

Q

A

V

I

S

M

S

A

G

A

A

P

S

T

S

T

I

S

A

Q

Q

Y

K

A

A

A

G

I

V

E

A

A

A

M

L

-

G

-

L

-

G

T

K

N

A

G

G

E

G

E

E

D

T

V

V

Q

A

I

E

M

M

R

V

N

K

A

A

Y

Y

N

R

Q

E

R

R

R

R

R

D

F

F

V

L

L

V

E

K

L

S

F

L

S

G

E

D

M

I

-

K

G

G

L

V

K

K

C

I

F

S

E

E

P

P

E

Q

G

G

A

A

F

F

Y

Y

I

L

F

F

P

I

C

D

I

F

K

S

E

A

-

Y

-

Y

-

G

-

S

-

E

-

A

-

E

-

G

F

F

G

G

M

L

T

I

S

N

D

D

-

S

-

S

E

S

F

L

A

A

N

L

R

Y

F

F

L

L

R

D

E

K

E

F

K

Q

V

V

A

A

I

M

I

V

P

P

G

G

T

D

A

A

F

F

G

G

D

D

C

-

G

-

E

D

G

S

F

C

L

I

R
|
R

V

I

S

S

Y

Y

A

A

Y

T

S

S

I

L

E

D

E

V

L

L

K

Q

E

A

A

A

L

V

G

E

R

K

L

I

A

R

N

K

F

A

V

L

E

E

R

P

L

L

R

R

binding 2-oxoglutaric acid: V15 (≠ I17), A36 (≠ V35), N173 (= N173), K236 (= K234), R375 (= R361)

1b5oA Thermus thermophilus aspartate aminotransferase single mutant 1 (see paper)
40% identity, 97% coverage: 5:382/391 of query aligns to 4:382/382 of 1b5oA

query
sites
1b5oA

L

L

S

S

D

R

K

R

V

V

V

Q

Q

A

M

M

K

K

P

P

S

S

G

A

I

T

-

V

-

A

-

V

-

N

R

A

K

K

F

A

F

L

D

E

L

L

V

R

Q

R

E

Q

M

G

P

V

D

D

A

L

I

V

S

A

L

L

G

T

V

A

G

G

E

E

P

P

D

D

F

F

D

D

T

T

P

P

W

E

H

H

I

V

R

K

E

E

E

A

G

A

I

R

Y

R

S

A

L

L

E

A

K

Q

G

G

R

K

T

T

F

K

Y

Y

T

A

S

P

N

P

A

A

G

G

L

I

L

P

E

E

L

L

R

R

K

E

A

A

I

L

A

A

H

E

Y

K

M

F

Y

R

R

R

K

E

Y

N

E

G

L

L

T

S

Y

V

N

T

P

P

A

-

H

E

E

E

I

T

V

I

V

V

T

T

V

V

G
|
G

G
|
G

S
|
S

E

Q

G

A

I

L

D

F

L

N

A

L

L

F

R

Q

A

A

M

I

L

L

N

D

P

P

G

G

D

D

E

E

V

V

I

I

L

V

P

L

E

S

P

P

A

Y

F
x
W

V

V

S

S

Y

Y

L

P

P

E

C

M

V

V

K

R

L

F

A

A

D

G

G

G

V

V

P

V

V

V

T

E

I

V

D

E

L

T

K

L

E

P

E

E

N

E

H

G

F

F

K

V

L

P

K

D

P

P

E

E

E

R

L

V

L

R

A

R

V

A

I

I

T

T

D

P

K

R

T

T

K

K

I

A

L

L

I

V

L

V

S

N

Y

S

P

P

N

N

N
|
N

P

P

T

T

G

G

A

A

I

V

M

Y

T

P

R

K

E

E

D

V

L

L

E

E

P

A

I

L

A

A

E

R

I

L

V

A

K

V

E

E

K

H

D

D

L

F

Y

Y

V

L

I

V

S

S

D
|
D

E

E

I
|
I

Y
|
Y

A

E

E

H

L

L

T

L

Y

Y

G

E

Q

G

D

E

H

H

C

F

S

S

I

-

A

-

S

-

L

-

P

P

G

G

-

R

-

V

M

A

R

P

D

E

R

H

T

T

I

L

I

T

I

V

N

N

G

G

F

A

S
x
A

K
|
K

S

A

F

F

A

A

M

M

T

T

G

G

W

W

R
|
R

M

I

G

G

F

Y

A

A

T

C

G

G

P

P

E

K

L

E

I

V

M

I

Q

K

Q

A

I

M

L

A

K

S

I

V

H

S

Q

S

F

Q

A

S

I

T

M

T

A

S

A

P

P

D

T

T

T

I

S

A

Q

Q

Y

W

A

A

A

T

I

L

E

E

A

A

M

L

T

T

N

N

G

Q

E

E

E

A

D

S

-

R

-

A

-

F

V

V

Q

E

I

M

M

A

R

R

N

E

A

A

Y

Y

N

R

Q

R

R

R

R

R

R

D

F

L

V

L

L

L

E

E

L

G

F

L

S

T

E

A

M

L

G

G

L

L

K

K

C

A

F

V

E

R

P

P

E

S

G

G

A

A

F

F

Y

Y

I

V

F

L

P

M

C

D

I

T

K

S

E

P

F

I

G

A

M

P

T

D

S

E

D

V

E

R

F

A

A

A

N

E

R

R

F

L

L

L

R

-

E

E

E

A

K

G

V

V

A

A

I

V

I

V

P

P

G

G

T

T

A

D

F

F

G

A

D

A

C

F

G

G

E

H

G

-

F

-

L

V

R

R

V

L

S

S

Y

Y

A

A

Y

T

S

S

I

E

E

E

E

N

L

L

K

R

E

K

A

A

L

L

G

E

R

R

L

F

A

A

N

R

F

V

V

L

active site: W125 (≠ F123), D203 (= D201), I205 (= I203), K234 (= K234)
binding pyridoxal-5'-phosphate: G99 (= G97), G100 (= G98), S101 (= S99), W125 (≠ F123), N175 (= N173), D203 (= D201), I205 (= I203), Y206 (= Y204), A233 (≠ S233), K234 (= K234), R242 (= R242)

5wmlA Arabidopsis thaliana prephenate aminotransferase mutant- k306a (see paper)
40% identity, 98% coverage: 5:386/391 of query aligns to 3:401/404 of 5wmlA

query
sites
5wmlA

L

L

S

S

D

P

K

R

V

V

V

Q

Q

S

M

L

K

K

P

P

S

S

G

K

I
x
T

R
x
M

K

V

F

I

F

T

D

D

L

L

-

A

-

A

-

T

-

L

V

V

Q

Q

E

S

M

G

P

V

D

P

A

V

I

I

S

R

L

L

G

A

V
x
A

G
|
G

E

E

P

P

D

D

F

F

D

D

T

T

P

P

W

K

H

V

I

V

R

A

E

E

E

A

G

G

I

I

Y

N

S

A

L

I

E

R

K

E

G

G

R

F

T

T

F

R

Y

Y

T

T

S

L

N

N

A

A

G

G

L

I

L

T

E

E

L

L

R

R

K

E

A

A

I

I

A

C

H

R

Y

K

M

L

Y

K

R

E

K

E

Y

N

E

G

L

L

T

S

Y

Y

N

A

P

P

A

-

H

D

E

Q

I

I

V

L

V

V

T

S

V

N

G
|
G

G
x
A

S
x
K

E

Q

G

S

I
x
L

D

L

L

Q

A

A

L

V

R

L

A

A

M

V

L

C

N

S

P

P

G

G

D

D

E

E

V

V

I

I

L

I

P

P

E

A

P

P

A

Y

F
x
W

V

V

S

S

Y

Y

L

T

P

E

C

Q

V

A

K

R

L

L

A

A

D

D

G

A

V

T

P

P

V

V

T

V

I

I

D

P

L

T

K

K

E

I

E

S

N

N

H

N

F

F

K

L

L

L

K

D

P

P

E

K

E

D

L

L

L

E

A

S

V

K

I

L

T

T

D

E

K

K

T

S

K

R

I

L

L

L

I

I

L

L

S

C

Y

S

P

P

N

S

N
|
N

P

P

T

T

G

G

A

S

I

V

M

Y

T

P

R

K

E

S

D

L

L

L

E

E

P

E

I

I

A

A

E

R

I

I

V

I

-

A

K

K

E

H

K

P

D

R

L

L

Y

L

V

V

I

L

S

S

D
|
D

E

E

I

I

Y
|
Y

A

E

E

H

L

I

T

I

Y

Y

G

A

-

P

Q

A

D

T

H

H

C

T

S

S

I

F

A

A

S

S

L

L

P

P

G

D

M

M

R

Y

D

E

R

R

T

T

I

L

I

T

I

V

N

N

G

G

F

F

S
|
S

K

A

S

A

F

F

A

A

M

M

T

T

G

G

W

W

R
|
R

M

L

G

G

F

Y

A

L

T

A

G

G

P

P

E

K

L

H

I

I

M

V

Q

A

Q

A

I

C

L

S

K

K

I

L

H

Q

Q

G

F

Q

A

V

I

S

M

S

A

G

A

A

P

S

T

S

T

I

S

A

Q

Q

Y

K

A

A

A

G

I

V

E

A

A

A

M

L

-

G

-

L

-

G

T

K

N

A

G

G

E

G

E

E

D

T

V

V

Q

A

I

E

M

M

R

V

N

K

A

A

Y

Y

N

R

Q

E

R

R

R

R

R

D

F

F

V

L

L

V

E

K

L

S

F

L

S

G

E

D

M

I

-

K

G

G

L

V

K

K

C

I

F

S

E

E

P

P

E

Q

G

G

A

A

F

F

Y
|
Y

I

L

F

F

P

I

C

D

I

F

K

S

E

A

-

Y

-

Y

-

G

-

S

-

E

-

A

-

E

-

G

F

F

G

G

M

L

T

I

S

N

D

D

-

S

-

S

E

S

F

L

A

A

N

L

R

Y

F

F

L

L

R

D

E

K

E

F

K

Q

V

V

A

A

I

M

I

V

P

P

G

G

T

D

A

A

F

F

G

G

D

D

C

-

G

-

E

D

G

S

F

C

L

I

R
|
R

V

I

S

S

Y

Y

A

A

Y

T

S

S

I

L

E

D

E

V

L

L

K

Q

E

A

A

A

L

V

G

E

R

K

L

I

A

R

N

K

F

A

V

L

E

E

R

P

L

L

R

R

binding glutamic acid: T15 (≠ I17), M16 (≠ R18), A37 (≠ V35), G38 (= G36), K100 (≠ S99), W124 (≠ F123), Y326 (= Y319), R376 (= R361)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G98 (= G97), A99 (≠ G98), K100 (≠ S99), L103 (≠ I102), W124 (≠ F123), N174 (= N173), D203 (= D201), Y206 (= Y204), S236 (= S233), R245 (= R242)

1gc4A Thermus thermophilus aspartate aminotransferase tetra mutant 2 complexed with aspartate (see paper)
40% identity, 97% coverage: 5:382/391 of query aligns to 4:382/382 of 1gc4A

query
sites
1gc4A

L

L

S

S

D

R

K

R

V

V

V

Q

Q

A

M

M

K

K

P

P

S

D

G

A

I

V

-

V

-

A

-

V

-

N

R

A

K

K

F

A

F

L

D

E

L

L

V

R

Q

R

E

Q

M

G

P

V

D

D

A

L

I

V

S

A

L

L

G

T

V

A

G

G

E

E

P

P

D

D

F

F

D

D

T

T

P

P

W

E

H

H

I

V

R

K

E

E

E

A

G

A

I

R

Y

R

S

A

L

L

E

A

K

Q

G

G

R

K

T

T

F

K

Y
|
Y

T

A

S

P

N

P

A

A

G

G

L

I

L

P

E

E

L

L

R

R

K

E

A

A

I

L

A

A

H

E

Y

K

M

F

Y

R

R

R

K

E

Y

N

E

G

L

L

T

S

Y

V

N

T

P

P

A

-

H

E

E

E

I

T

V

I

V

V

T

T

V

V

G
|
G

G
|
G

S
|
S

E

Q

G

A

I

L

D

F

L

N

A

L

L

F

R

Q

A

A

M

I

L

L

N

D

P

P

G

G

D

D

E

E

V

V

I

I

L

V

P

L

E

S

P

P

A

Y

F
x
W

V

V

S

S

Y

Y

L

P

P

E

C

M

V

V

K

R

L

F

A

A

D

G

G

G

V

V

P

V

V

V

T

E

I

V

D

E

L

T

K

L

E

P

E

E

N

E

H

G

F

F

K

V

L

P

K

D

P

P

E

E

E

R

L

V

L

R

A

R

V

A

I

I

T

T

D

P

K

R

T

T

K

K

I

A

L

L

I

V

L

V

S

N

Y

S

P

P

N

N

N
|
N

P

P

T

T

G

G

A

A

I

V

M

Y

T

P

R

K

E

E

D

V

L

L

E

E

P

A

I

L

A

A

E

R

I

L

V

A

K

V

E

E

K

H

D

D

L

F

Y

Y

V

L

I

V

S

S

D
|
D

E

E

I

I

Y
|
Y

A

E

E

H

L

L

T

L

Y

Y

G

E

Q

G

D

E

H

H

C

F

S

S

I

-

A

-

S

-

L

-

P

P

G

G

-

R

-

V

M

A

R

P

D

E

R

H

T

T

I

L

I

T

I

V

N

N

G

G

F

A

S

A

K

K

S

A

F

F

A

A

M

M

T

T

G

G

W

W

R
|
R

M

I

G

G

F

Y

A

A

T

C

G

G

P

P

E

K

L

E

I

V

M

I

Q

K

Q

A

I

M

L

A

K

S

I

V

H

S

Q
x
R

F

Q

A

S

I

T

M

T

A

S

A

P

P

D

T

T

T

I

S

A

Q

Q

Y

W

A

A

A

T

I

L

E

E

A

A

M

L

T

T

N

N

G

Q

E

E

E

A

D

S

-

R

-

A

-

F

V

V

Q

E

I

M

M

A

R

R

N

E

A

A

Y

Y

N

R

Q

R

R

R

R

R

R

D

F

L

V

L

L

L

E

E

L

G

F

L

S

T

E

A

M

L

G

G

L

L

K

K

C

A

F

V

E

R

P

P

E

S

G

G

A

A

F

F

Y
|
Y

I

V

F

L

P

M

C

D

I

T

K

S

E

P

F

I

G

A

M

P

T

D

S

E

D

V

E

R

F

A

A

A

N

E

R

R

F

L

L

L

R

-

E

E

E

A

K

G

V

V

A

A

I

V

I

V

P

P

G

G

T

T

A

D

F

F

G

A

D

A

C

F

G

G

E

H

G

-

F

-

L

V

R
|
R

V

L

S

S

Y

Y

A

A

Y

T

S

S

I

E

E

E

E

N

L

L

K

R

E

K

A

A

L

L

G

E

R

R

L

F

A

A

N

R

F

V

V

L

binding aspartic acid: Y64 (= Y61), W125 (≠ F123), N175 (= N173), R261 (≠ Q261), Y322 (= Y319), R361 (= R361)
binding pyridoxal-5'-phosphate: G99 (= G97), G100 (= G98), S101 (= S99), W125 (≠ F123), N175 (= N173), D203 (= D201), Y206 (= Y204), R242 (= R242)

1gc3A Thermus thermophilus aspartate aminotransferase tetra mutant 2 complexed with tryptophan (see paper)
40% identity, 97% coverage: 5:382/391 of query aligns to 4:382/382 of 1gc3A

query
sites
1gc3A

L

L

S

S

D

R

K

R

V

V

V

Q

Q

A

M

M

K

K

P

P

S

D

G

A

I
x
V

-

V

-

A

-

V

-
x
N

R

A

K

K

F

A

F

L

D

E

L

L

V

R

Q

R

E

Q

M

G

P

V

D

D

A

L

I

V

S

A

L

L

G
x
T

V
x
A

G
|
G

E
|
E

P

P

D

D

F

F

D

D

T

T

P

P

W

E

H

H

I

V

R

K

E

E

E

A

G

A

I

R

Y

R

S

A

L

L

E

A

K

Q

G

G

R

K

T

T

F

K

Y

Y

T

A

S

P

N

P

A

A

G

G

L

I

L

P

E

E

L

L

R

R

K

E

A

A

I

L

A

A

H

E

Y

K

M

F

Y

R

R

R

K

E

Y

N

E

G

L

L

T

S

Y

V

N

T

P

P

A

-

H

E

E

E

I

T

V

I

V

V

T

T

V

V

G

G

G
|
G

S
|
S

E

Q

G

A

I

L

D

F

L

N

A

L

L

F

R

Q

A

A

M

I

L

L

N

D

P

P

G

G

D

D

E

E

V

V

I

I

L

V

P

L

E

S

P

P

A

Y

F
x
W

V

V

S

S

Y

Y

L

P

P

E

C

M

V

V

K

R

L

F

A

A

D

G

G

G

V

V

P

V

V

V

T

E

I

V

D

E

L

T

K

L

E

P

E

E

N

E

H

G

F

F

K

V

L

P

K

D

P

P

E

E

E

R

L

V

L

R

A

R

V

A

I

I

T

T

D

P

K

R

T

T

K

K

I

A

L

L

I

V

L

V

S

N

Y

S

P

P

N

N

N
|
N

P

P

T

T

G

G

A

A

I

V

M

Y

T

P

R

K

E

E

D

V

L

L

E

E

P

A

I

L

A

A

E

R

I

L

V

A

K

V

E

E

K

H

D

D

L

F

Y

Y

V

L

I

V

S

S

D
|
D

E

E

I

I

Y
|
Y

A

E

E

H

L

L

T

L

Y

Y

G

E

Q

G

D

E

H

H

C

F

S

S

I

-

A

-

S

-

L

-

P

P

G

G

-

R

-

V

M

A

R

P

D

E

R

H

T

T

I

L

I

T

I

V

N

N

G

G

F

A

S

A

K
|
K

S

A

F

F

A

A

M

M

T

T

G

G

W

W

R
|
R

M

I

G

G

F

Y

A

A

T

C

G

G

P

P

E

K

L

E

I

V

M

I

Q

K

Q

A

I

M

L

A

K

S

I

V

H

S

Q

R

F

Q

A

S

I

T

M

T

A

S

A

P

P

D

T

T

T

I

S

A

Q

Q

Y

W

A

A

A

T

I

L

E

E

A

A

M

L

T

T

N

N

G

Q

E

E

E

A

D

S

-

R

-

A

-

F

V

V

Q

E

I

M

M

A

R

R

N

E

A

A

Y

Y

N

R

Q

R

R

R

R

R

R

D

F

L

V

L

L

L

E

E

L

G

F

L

S

T

E

A

M

L

G

G

L

L

K

K

C

A

F

V

E

R

P

P

E

S

G

G

A

A

F

F

Y
|
Y

I

V

F

L

P

M

C

D

I

T

K

S

E

P

F

I

G

A

M

P

T

D

S

E

D

V

E

R

F

A

A

A

N

E

R

R

F

L

L

L

R

-

E

E

E

A

K

G

V

V

A

A

I

V

I

V

P

P

G

G

T

T

A

D

F

F

G

A

D

A

C

F

G

G

E

H

G

-

F

-

L

V

R
|
R

V

L

S

S

Y

Y

A

A

Y

T

S

S

I

E

E

E

E

N

L

L

K

R

E

K

A

A

L

L

G

E

R

R

L

F

A

A

N

R

F

V

V

L

binding pyridoxal-5'-phosphate: G100 (= G98), S101 (= S99), W125 (≠ F123), N175 (= N173), D203 (= D201), Y206 (= Y204), K234 (= K234), R242 (= R242)
binding tryptophan: V16 (≠ I17), N20 (vs. gap), T37 (≠ G34), A38 (≠ V35), G39 (= G36), E40 (= E37), Y322 (= Y319), R361 (= R361)

5wmhA Arabidopsis thaliana prephenate aminotransferase (see paper)
40% identity, 98% coverage: 5:387/391 of query aligns to 2:394/399 of 5wmhA

query
sites
5wmhA

L

L

S

S

D

P

K

R

V

V

V

Q

Q

S

M

L

K

K

P

P

S

S

G

K

I

T

R

M

K

V

F

I

F

T

D

D

L

L

-

A

-

A

-

T

-

L

V

V

Q

Q

E

S

M

G

P

V

D

P

A

V

I

I

S

R

L

L

G

A

V

A

G

G

E

E

P

P

D

D

F

F

D

D

T

T

P

P

W

K

H

V

I

V

R

A

E

E

E

A

G

G

I

I

Y

N

S

A

L

I

E

R

K

E

G

G

R

F

T

T

F

R

Y

Y

T

T

S

L

N

N

A

A

G

G

L

I

L

T

E

E

L

L

R

R

K

E

A

A

I

I

A

C

H

R

Y

K

M

L

Y

K

R

E

K

E

Y

N

E

G

L

L

T

S

Y

Y

N

A

P

P

A

-

H

D

E

Q

I

I

V

L

V

V

T

S

V

N

G
|
G

G
x
A

S
x
K

E

Q

G

S

I

L

D

L

L

Q

A

A

L

V

R

L

A

A

M

V

L

C

N

S

P

P

G

G

D

D

E

E

V

V

I

I

L

I

P

P

E

A

P

P

A

Y

F
x
W

V

V

S

S

Y

Y

L

T

P

E

C

Q

V

A

K

R

L

L

A

A

D

D

G

A

V

T

P

P

V

V

T

V

I

I

D

P

L

T

K

K

E

I

E

S

N

N

H

N

F

F

K

L

L

L

K

D

P

P

E

K

E

D

L

L

L

E

A

S

V

K

I

L

T

T

D

E

K

K

T

S

K

R

I

L

L

L

I

I

L

L

S

C

Y

S

P

P

N

S

N
|
N

P

P

T

T

G

G

A

S

I

V

M

Y

T

P

R

K

E

S

D

L

L

L

E

E

P

E

I

I

A

A

E

R

I

I

V

I

-

A

K

K

E

H

K

P

D

R

L

L

Y

L

V

V

I

L

S

S

D
|
D

E

E

I
|
I

Y
|
Y

A

E

E

H

L

I

T

I

Y

Y

G

A

-

P

Q

A

D

T

H

H

C

T

S

S

I

F

A

A

S

S

L

L

P

P

G

D

M

M

R

Y

D

E

R

R

T

T

I

L

I

T

I

V

N

N

G

G

F

F

S
|
S

K
|
K

S

A

F

F

A

A

M

M

T

T

G

G

W

W

R
|
R

M

L

G

G

F

Y

A

L

T

A

G

G

P

P

E

K

L

H

I

I

M

V

Q

A

Q

A

I

C

L

S

K

K

I

L

H

Q

Q

G

F

Q

A

V

I

S

M

S

A

G

A

A

P

S

T

S

T

I

S

A

Q

Q

Y

K

A

A

A

G

I

V

E

A

A

A

M

L

-

G

-

L

-

G

T

K

N

A

G

G

E

G

E

E

D

T

V

V

Q

A

I

E

M

M

R

V

N

K

A

A

Y

Y

N

R

Q

E

R

R

R

R

R

D

F

F

V

L

L

V

E

K

L

S

F

L

S

G

E

D

M

I

-

K

G

G

L

V

K

K

C

I

F

S

E

E

P

P

E

Q

G

G

A

A

F

F

Y

Y

I

L

F

F

P

I

C

D

I

F

K

S

E

A

-

Y

-

Y

-

G

-

S

-

E

-

A

-

E

-

G

F

F

G

G

M

L

T

I

S

N

D

D

-

S

-

S

E

S

F

L

A

A

N

L

R

Y

F

F

L

L

R

D

E

K

E

F

K

Q

V

V

A

A

I

M

I

V

P

P

G

G

T

D

A

A

F

F

G

G

D

D

C

-

G

-

E

D

G

S

F

C

L

I

R

R

V

I

S

S

Y

Y

A

A

Y

T

S

S

I

L

E

D

E

V

L

L

K

Q

E

A

A

A

L

-

G

-

R

-

L

-

A

-

N

-

F

-

V

V

E

E

R

K

L

I

R

R

K

K

binding pyridoxal-5'-phosphate: G97 (= G97), A98 (≠ G98), K99 (≠ S99), W123 (≠ F123), N173 (= N173), D202 (= D201), I204 (= I203), Y205 (= Y204), S235 (= S233), K236 (= K234), R244 (= R242)

Q56232 Aspartate/prephenate aminotransferase; AspAT / PAT; Transaminase A; EC 2.6.1.1; EC 2.6.1.78 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see 3 papers)
40% identity, 97% coverage: 5:384/391 of query aligns to 4:384/385 of Q56232

query
sites
Q56232

L

L

S

S

D

R

K

R

V

V

V

Q

Q

A

M

M

K
|
K

P

P

S

S

G

A

I

T

-

V

-

A

-

V

-

N

R

A

K

K

F

A

F

L

D

E

L

L

V

R

Q

R

E

Q

M

G

P

V

D

D

A

L

I

V

S

A

L

L

G

T

V

A

G

G

E

E

P

P

D

D

F

F

D

D

T

T

P

P

W

E

H

H

I

V

R

K

E

E

E

A

G

A

I

R

Y

R

S

A

L

L

E

A

K

Q

G

G

R

K

T

T

F

K

Y

Y

T

A

S

P

N

P

A

A

G

G

L

I

L

P

E

E

L

L

R

R

K

E

A

A

I

L

A

A

H

E

Y

K

M

F

Y

R

R

R

K

E

Y

N

E

G

L

L

T

S

Y

V

N

T

P

P

A

-

H

E

E

E

I

T

V

I

V

V

T

T

V

V

G

G

G

G

S

K

E

Q

G

A

I

L

D

F

L

N

A

L

L

F

R

Q

A

A

M

I

L

L

N

D

P

P

G

G

D

D

E

E

V

V

I

I

L

V

P

L

E

S

P

P

A

Y

F

W

V

V

S

S

Y

Y

L

P

P

E

C

M

V

V

K

R

L

F

A

A

D

G

G

G

V

V

P

V

V

V

T

E

I

V

D

E

L

T

K

L

E

P

E

E

N

E

H

G

F

F

K

V

L

P

K

D

P

P

E

E

E

R

L

V

L

R

A

R

V

A

I

I

T

T

D

P

K

R

T

T

K

K

I

A

L

L

I

V

L

V

S

N

Y

S

P

P

N

N

N

N

P

P

T

T

G

G

A

A

I

V

M

Y

T

P

R

K

E

E

D

V

L

L

E

E

P

A

I

L

A

A

E

R

I

L

V

A

K

V

E

E

K

H

D

D

L

F

Y

Y

V

L

I

V

S

S

D

D

E

E

I

I

Y

Y

A

E

E

H

L

L

T

L

Y

Y

G

E

Q

G

D

E

H

H

C

F

S

S

I

-

A

-

S

-

L

-

P

P

G

G

-

R

-

V

M

A

R

P

D

E

R

H

T

T

I

L

I

T

I

V

N

N

G

G

F

A

S

A

K
|
K

S

A

F

F

A

A

M

M

T

T

G

G

W

W

R

R

M

I

G

G

F

Y

A

A

T

C

G

G

P

P

E

K

L

E

I

V

M

I

Q

K

Q

A

I

M

L

A

K

S

I

V

H

S

Q

S

F

Q

A

S

I

T

M

T

A

S

A

P

P

D

T

T

T

I

S

A

Q

Q

Y

W

A

A

A

T

I

L

E

E

A

A

M

L

T

T

N

N

G

Q

E

E

E

A

D

S

-

R

-

A

-

F

V

V

Q

E

I

M

M

A

R

R

N

E

A

A

Y

Y

N

R

Q

R

R

R

R

R

R

D

F

L

V

L

L

L

E

E

L

G

F

L

S

T

E

A

M

L

G

G

L

L

K

K

C

A

F

V

E

R

P

P

E

S

G

G

A

A

F

F

Y

Y

I

V

F

L

P

M

C

D

I

T

K

S

E

P

F

I

G

A

M

P

T

D

S

E

D

V

E

R

F

A

A

A

N

E

R

R

F

L

L

L

R

-

E

E

E

A

K

G

V

V

A

A

I

V

I

V

P

P

G

G

T

T

A

D

F

F

G

A

D

A

C

F

G

G

E

H

G

-

F

-

L

V

R

R

V

L

S

S

Y

Y

A

A

Y

T

S

S

I

E

E

E

E

N

L

L

K

R

E

K

A

A

L

L

G

E

R

R

L

F

A

A

N

R

F

V

V

L

E

G

R

R

K12 (= K13) Important for prephenate aminotransferase activity; mutation to G: 10-fold increase in Km for prephenate. Does not affect Km for oxaloacetate.
K234 (= K234) modified: N6-(pyridoxal phosphate)lysine

1bkgA Aspartate aminotransferase from thermus thermophilus with maleate (see paper)
40% identity, 97% coverage: 5:382/391 of query aligns to 4:382/382 of 1bkgA

query
sites
1bkgA

L

L

S

S

D

R

K

R

V

V

V

Q

Q

A

M

M

K

K

P

P

S

S

G

A

I

T

-

V

-

A

-

V

-

N

R

A

K

K

F

A

F

L

D

E

L

L

V

R

Q

R

E

Q

M

G

P

V

D

D

A

L

I

V

S

A

L

L

G

T

V

A

G

G

E

E

P

P

D

D

F

F

D

D

T

T

P

P

W

E

H

H

I

V

R

K

E

E

E

A

G

A

I

R

Y

R

S

A

L

L

E

A

K

Q

G

G

R

K

T

T

F

K

Y

Y

T

A

S

P

N

P

A

A

G

G

L

I

L

P

E

E

L

L

R

R

K

E

A

A

I

L

A

A

H

E

Y

K

M

F

Y

R

R

R

K

E

Y

N

E

G

L

L

T

S

Y

V

N

T

P

P

A

-

H

E

E

E

I

T

V

I

V

V

T

T

V

V

G
|
G

G
|
G

S
x
K

E

Q

G

A

I

L

D

F

L

N

A

L

L

F

R

Q

A

A

M

I

L

L

N

D

P

P

G

G

D

D

E

E

V

V

I

I

L

V

P

L

E

S

P

P

A

Y

F
x
W

V

V

S

S

Y

Y

L

P

P

E

C

M

V

V

K

R

L

F

A

A

D

G

G

G

V

V

P

V

V

V

T

E

I

V

D

E

L

T

K

L

E

P

E

E

N

E

H

G

F

F

K

V

L

P

K

D

P

P

E

E

E

R

L

V

L

R

A

R

V

A

I

I

T

T

D

P

K

R

T

T

K

K

I

A

L

L

I

V

L

V

S
x
N

Y

S

P

P

N

N

N
|
N

P

P

T

T

G

G

A

A

I

V

M

Y

T

P

R

K

E

E

D

V

L

L

E

E

P

A

I

L

A

A

E

R

I

L

V

A

K

V

E

E

K

H

D

D

L

F

Y

Y

V

L

I

V

S

S

D
|
D

E

E

I
|
I

Y
|
Y

A

E

E

H

L

L

T

L

Y

Y

G

E

Q

G

D

E

H

H

C

F

S

S

I

-

A

-

S

-

L

-

P

P

G

G

-

R

-

V

M

A

R

P

D

E

R

H

T

T

I

L

I

T

I

V

N

N

G

G

F

A

S

A

K
|
K

S

A

F

F

A

A

M

M

T

T

G

G

W

W

R
|
R

M

I

G

G

F

Y

A

A

T

C

G

G

P

P

E

K

L

E

I

V

M

I

Q

K

Q

A

I

M

L

A

K

S

I

V

H

S

Q

S

F

Q

A

S

I

T

M

T

A

S

A

P

P

D

T

T

T

I

S

A

Q

Q

Y

W

A

A

A

T

I

L

E

E

A

A

M

L

T

T

N

N

G

Q

E

E

E

A

D

S

-

R

-

A

-

F

V

V

Q

E

I

M

M

A

R

R

N

E

A

A

Y

Y

N

R

Q

R

R

R

R

R

R

D

F

L

V

L

L

L

E

E

L

G

F

L

S

T

E

A

M

L

G

G

L

L

K

K

C

A

F

V

E

R

P

P

E

S

G

G

A

A

F

F

Y

Y

I

V

F

L

P

M

C

D

I

T

K

S

E

P

F

I

G

A

M

P

T

D

S

E

D

V

E

R

F

A

A

A

N

E

R

R

F

L

L

L

R

-

E

E

E

A

K

G

V

V

A

A

I

V

I

V

P

P

G

G

T

T

A

D

F

F

G

A

D

A

C

F

G

G

E

H

G

-

F

-

L

V

R

R

V

L

S

S

Y

Y

A

A

Y

T

S

S

I

E

E

E

E

N

L

L

K

R

E

K

A

A

L

L

G

E

R

R

L

F

A

A

N

R

F

V

V

L

active site: W125 (≠ F123), D203 (= D201), I205 (= I203), K234 (= K234)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G99 (= G97), G100 (= G98), K101 (≠ S99), W125 (≠ F123), N171 (≠ S169), N175 (= N173), D203 (= D201), I205 (= I203), Y206 (= Y204), K234 (= K234), R242 (= R242)

1bjwA Aspartate aminotransferase from thermus thermophilus (see paper)
40% identity, 97% coverage: 5:382/391 of query aligns to 4:382/382 of 1bjwA

query
sites
1bjwA

L

L

S

S

D

R

K

R

V

V

V

Q

Q

A

M

M

K

K

P

P

S

S

G

A

I

T

-

V

-

A

-

V

-

N

R

A

K

K

F

A

F

L

D

E

L

L

V

R

Q

R

E

Q

M

G

P

V

D

D

A

L

I

V

S

A

L

L

G

T

V

A

G
|
G

E

E

P

P

D

D

F

F

D

D

T

T

P

P

W

E

H

H

I

V

R

K

E

E

E

A

G

A

I

R

Y

R

S

A

L

L

E

A

K

Q

G

G

R

K

T

T

F

K

Y

Y

T

A

S

P

N

P

A

A

G

G

L

I

L

P

E

E

L

L

R

R

K

E

A

A

I

L

A

A

H

E

Y

K

M

F

Y

R

R

R

K

E

Y

N

E

G

L

L

T

S

Y

V

N

T

P

P

A

-

H

E

E

E

I

T

V

I

V

V

T

T

V

V

G

G

G

G

S

K

E

Q

G

A

I

L

D

F

L

N

A

L

L

F

R

Q

A

A

M

I

L

L

N

D

P

P

G

G

D

D

E

E

V

V

I

I

L

V

P

L

E

S

P

P

A

Y

F
x
W

V

V

S

S

Y

Y

L

P

P

E

C

M

V

V

K

R

L

F

A

A

D

G

G

G

V

V

P

V

V

V

T

E

I

V

D

E

L

T

K

L

E

P

E

E

N

E

H

G

F

F

K

V

L

P

K

D

P

P

E

E

E

R

L

V

L

R

A

R

V

A

I

I

T

T

D

P

K

R

T

T

K

K

I

A

L

L

I

V

L

V

S

N

Y

S

P

P

N

N

N
|
N

P

P

T

T

G

G

A

A

I

V

M

Y

T

P

R

K

E

E

D

V

L

L

E

E

P

A

I

L

A

A

E

R

I

L

V

A

K

V

E

E

K

H

D

D

L

F

Y

Y

V

L

I

V

S

S

D
|
D

E

E

I
|
I

Y

Y

A

E

E

H

L

L

T

L

Y

Y

G

E

Q

G

D

E

H

H

C

F

S

S

I

-

A

-

S

-

L

-

P

P

G

G

-

R

-

V

M

A

R

P

D

E

R

H

T

T

I

L

I

T

I

V

N

N

G

G

F

A

S

A

K
|
K

S

A

F

F

A

A

M

M

T

T

G

G

W

W

R

R

M

I

G

G

F

Y

A

A

T

C

G

G

P

P

E

K

L

E

I

V

M

I

Q

K

Q

A

I

M

L

A

K

S

I

V

H

S

Q

S

F

Q

A

S

I

T

M

T

A

S

A

P