SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_005345831.1 NCBI__GCF_000173975.1:WP_005345831.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 16 hits to proteins with known functional sites (download)

6wyeA Crystal structure of neisseria gonorrhoeae serine acetyltransferase (cyse) (see paper)
33% identity, 64% coverage: 42:138/152 of query aligns to 125:221/261 of 6wyeA

query
sites
6wyeA

G

G

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binding (2S)-2-hydroxybutanedioic acid: D158 (≠ A75), H159 (= H76), G185 (≠ Q102), R193 (≠ G110), H194 (≠ T111)

Sites not aligning to the query:

binding (2S)-2-hydroxybutanedioic acid: 93, 94

7ra4A Crystal structure of neisseria gonorrhoeae serine acetyltransferase (cyse) in complex with serine (see paper)
33% identity, 64% coverage: 42:138/152 of query aligns to 123:219/243 of 7ra4A

query
sites
7ra4A

G

G

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binding serine: D156 (≠ A75), H157 (= H76), R191 (≠ G110), H192 (≠ T111)

Sites not aligning to the query:

binding serine: 91, 92

4n69A Soybean serine acetyltransferase complexed with serine (see paper)
38% identity, 50% coverage: 59:134/152 of query aligns to 140:215/243 of 4n69A

query
sites
4n69A

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binding serine: G183 (≠ Q102), R191 (≠ G110), H192 (≠ T111)

7bw9A Crystal structure of serine acetyltransferase isoform 3 in complex with cysteine from entamoeba histolytica
36% identity, 57% coverage: 59:145/152 of query aligns to 163:249/280 of 7bw9A

query
sites
7bw9A

G

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binding cysteine: D179 (≠ A75), H180 (= H76), A206 (≠ Q102), R214 (≠ G110), H215 (≠ T111)

Sites not aligning to the query:

binding cysteine: 114, 115

4n6bA Soybean serine acetyltransferase complexed with coa (see paper)
41% identity, 50% coverage: 59:134/152 of query aligns to 133:205/233 of 4n6bA

query
sites
4n6bA

G

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binding coenzyme a: H150 (= H76), L169 (≠ H95), G176 (≠ Q102)

Sites not aligning to the query:

binding coenzyme a: 208, 210, 211, 216, 226

3p47A Crystal structure of entamoeba histolytica serine acetyltransferase 1 in complex with l-cysteine (see paper)
36% identity, 57% coverage: 59:145/152 of query aligns to 165:259/270 of 3p47A

query
sites
3p47A

G

S

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binding cysteine: A208 (vs. gap), H210 (vs. gap), R224 (vs. gap), H225 (vs. gap)

3q1xA Crystal structure of entamoeba histolytica serine acetyltransferase 1 in complex with l-serine (see paper)
36% identity, 57% coverage: 59:145/152 of query aligns to 163:257/267 of 3q1xA

query
sites
3q1xA

G

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binding serine: H208 (vs. gap), R222 (vs. gap), H223 (vs. gap)

4hzdA Crystal structure of serine acetyltransferase in complex with coenzyme a from brucella abortus strain s19 (see paper)
28% identity, 66% coverage: 42:142/152 of query aligns to 124:224/250 of 4hzdA

query
sites
4hzdA

G

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S

binding coenzyme a: H158 (= H76), G203 (= G121), A204 (≠ M122), A221 (= A139), A222 (≠ P140)
binding magnesium ion: S224 (≠ T142)

Sites not aligning to the query:

8i04A Crystal structure of serine acetyltransferase from salmonella typhimurium complexed with serine (see paper)
31% identity, 49% coverage: 60:133/152 of query aligns to 138:211/258 of 8i04A

query
sites
8i04A

I

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G

binding serine: D153 (≠ A75), H154 (= H76), G180 (≠ Q102), R188 (≠ G110), H189 (≠ T111)

Sites not aligning to the query:

binding serine: 88, 89

1ssqD Serine acetyltransferase- complex with cysteine (see paper)
30% identity, 55% coverage: 60:142/152 of query aligns to 138:220/257 of 1ssqD

query
sites
1ssqD

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S

binding cysteine: D153 (≠ A75), H154 (= H76)

Sites not aligning to the query:

binding cysteine: 88

8i09A Crystal structure of serine acetyltransferase from salmonella typhimurium complexed with butyl gallate (see paper)
31% identity, 49% coverage: 60:133/152 of query aligns to 141:214/246 of 8i09A

query
sites
8i09A

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G

binding cysteine: D156 (≠ A75), H157 (= H76), G164 (≠ N83), R191 (≠ G110), H192 (≠ T111)
binding butyl 3,4,5-tris(oxidanyl)benzoate: G183 (≠ Q102), T184 (≠ E103), G202 (= G121)

Sites not aligning to the query:

8i06A Crystal structure of serine acetyltransferase from salmonella typhimurium complexed with coa (see paper)
31% identity, 49% coverage: 60:133/152 of query aligns to 142:215/244 of 8i06A

query
sites
8i06A

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G

binding coenzyme a: H158 (= H76), L177 (≠ H95), Q178 (≠ K96), G203 (= G121), A204 (≠ M122)
binding cysteine: H158 (= H76)

Sites not aligning to the query:

binding coenzyme a: 221, 222, 235, 237
binding cysteine: 93

1t3dA Crystal structure of serine acetyltransferase from e.Coli at 2.2a (see paper)
31% identity, 49% coverage: 60:133/152 of query aligns to 142:215/262 of 1t3dA

query
sites
1t3dA

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E

K

T

C

A

I

V

I

I

G

E

M

N

N

D

C

V

N

S

I

I

H

L

K

Q

G

S

V

V

T

T

I

L

G

G

Q
x
G

E

T

N

G

R

K

G

S

K

G

R

G

Q

D

G
x
R

T
x
H

P

P

I

K

I

I

G

R

D

E

N

G

V

V

W

M

I

I

G

G

M

A

N

G

V

A

T

K

I

I

V

L

G

G

N

N

I

I

K

E

I

V

G

G

binding cysteine: D157 (≠ A75), G184 (≠ Q102), R192 (≠ G110), H193 (≠ T111)

Sites not aligning to the query:

binding cysteine: 92, 93

3gvdI Crystal structure of serine acetyltransferase cyse from yersinia pestis
31% identity, 49% coverage: 59:133/152 of query aligns to 144:218/272 of 3gvdI

query
sites
3gvdI

G

G

I

V

E

D

I

I

D

H

Y

P

P

A

V

A

K

T

I

I

G

G

K

C

G

G

L

I

F

M

I

L

A
x
D

H

H

P

A

Y

T

G

G

I

I

T

V

I

I

N

G

D

E

K

T

C

A

I

V

I

V

G

E

M

N

N

D

C

V

N

S

I

I

H

L

K

Q

G

S

V

V

T

T

I

L

G
|
G

Q
x
G

E

T

N

G

R

K

G

T

K

S

R

G

Q

D

G
x
R

T

H

P

P

I

K

I

I

G

R

D

E

N

G

V

V

W

M

I

I

G

G

M

A

N

G

V

A

T

K

I

I

V

L

G

G

N

N

I

I

K

E

I

V

G

G

binding cysteine: D160 (≠ A75), G186 (= G101), G187 (≠ Q102), R195 (≠ G110)

Sites not aligning to the query:

binding cysteine: 95, 96

4h7oA Crystal structure of serine acetyltransferase from vibrio cholerae o1 biovar el tor n16961
29% identity, 49% coverage: 60:134/152 of query aligns to 138:212/258 of 4h7oA

query
sites
4h7oA

I

V

E

D

I

I

D

H

Y

P

P

A

V

A

K

R

I

I

G

G

K

R

G

G

L

I

F

M

I

L

A
x
D

H
|
H

P

A

Y

T

G

G

I

I

T

V

I

I

N

G

D

E

K

T

C

A

I

V

I

V

G

E

M

D

N

D

C

V

N

S

I

I

H

L

K

Q

G

D

V

V

T

T

I

L

G

G

Q
x
G

E

T

N

G

R

K

G

E

K

C

R

G

Q

D

G
x
R

T
x
H

P

P

I

K

I

I

G

R

D

E

N

G

V

V

W

M

I

I

G

G

M

A

N

G

V

A

T

K

I

I

V

L

G

G

N

N

I

I

K

E

I

V

G

G

N

E

binding cysteine: D153 (≠ A75), H154 (= H76), G180 (≠ Q102), R188 (≠ G110), H189 (≠ T111)

Sites not aligning to the query:

binding arginine: 51, 53, 127, 131
binding cysteine: 88, 89

1sstA Serine acetyltransferase- complex with coa (see paper)
31% identity, 55% coverage: 60:142/152 of query aligns to 138:213/233 of 1sstA

query
sites
1sstA

I

V

E

D

I

I

D

H

Y

P

P

A

V

A

K

K

I

I

G

G

K

H

G

G

L

I

F

M

I

F

A

D

H
|
H

P

A

Y

T

G

G

I

I

T

V

I

V

N

G

D

E

K

T

C

S

I

V

I

I

G

E

M

N

N

D

C

V

N

S

I

I

H
x
L

K

Q

G

G

V

V

T

T

I

L

G

G

Q
x
G

E
x
T

N

H

R

P

G

K

K

V

R

R

Q

E

G

G

T

-

P

-

I

-

I

-

G

-

D

-

N

-

V

V

W

M

I

I

G

G

M
x
A

N

G

V

A

T

K

I

I

V
x
L

G

G

N

N

I

I

K

E

I

V

G

G

N

K

D

Y

V

A

L
x
K

I

I

A
x
G

P
x
A

N

N

T

S

binding coenzyme a: H154 (= H76), L173 (≠ H95), G180 (≠ Q102), T181 (≠ E103), A193 (≠ M122), L198 (≠ V127), K208 (≠ L137), G210 (≠ A139), A211 (≠ P140)

Sites not aligning to the query:

binding coenzyme a: 214, 216, 224, 226, 228, 229, 233

Query Sequence

>WP_005345831.1 NCBI__GCF_000173975.1:WP_005345831.1
MEMSGWRAVFEADMYRYGASKVDKYIKKWNYFFRRCQYASGGKKIIWHLLLLKHGTKHGI
EIDYPVKIGKGLFIAHPYGITINDKCIIGMNCNIHKGVTIGQENRGKRQGTPIIGDNVWI
GMNVTIVGNIKIGNDVLIAPNTYLNLMYQVTR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory