SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_005346048.1 NCBI__GCF_000173975.1:WP_005346048.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 5 hits to proteins with known functional sites (download)

4gyfA Crystal structure of histidinol phosphate phosphatase (hisk) from lactococcus lactis subsp. Lactis il1403 complexed with zn, l- histidinol and phosphate
32% identity, 97% coverage: 4:277/283 of query aligns to 6:260/263 of 4gyfA

query
sites
4gyfA

D

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binding L-histidinol: H31 (≠ L29), E114 (= E133), Y156 (≠ L174), Y160 (= Y178)
binding zinc ion: H8 (= H6), H10 (= H8), D16 (= D14), H31 (≠ L29), H41 (= H39), E80 (= E99), E80 (= E99), H108 (= H127), H153 (= H171), D227 (= D244), H229 (= H246), H239 (≠ D256)

Sites not aligning to the query:

binding L-histidinol: 4

4gk8A Crystal structure of histidinol phosphate phosphatase (hisk) from lactococcus lactis subsp. Lactis il1403 complexed with zn and l- histidinol arsenate (see paper)
32% identity, 97% coverage: 4:277/283 of query aligns to 6:260/263 of 4gk8A

query
sites
4gk8A

D

D

Y

Y

H
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H

V

F

H
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H

T

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binding {[(2S)-2-amino-3-(1H-imidazol-5-yl)propyl]oxy}(trihydroxy)-lambda~5~-arsanyl: H10 (= H8), D16 (= D14), H41 (= H39), H108 (= H127), E114 (= E133), Y156 (≠ L174), R159 (= R177), D227 (= D244), H229 (= H246)
binding zinc ion: H8 (= H6), H10 (= H8), D16 (= D14), H31 (≠ L29), H41 (= H39), E80 (= E99), E80 (= E99), H108 (= H127), H153 (= H171), D227 (= D244), H229 (= H246), H239 (≠ D256)

4gc3A Crystal structure of l-histidinol phosphate phosphatase (hisk) from lactococcus lactis subsp. Lactis il1403 complexed with zn and sulfate (see paper)
32% identity, 97% coverage: 4:277/283 of query aligns to 6:260/263 of 4gc3A

query
sites
4gc3A

D

D

Y

Y

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H

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H

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binding sulfate ion: H41 (= H39), Y156 (≠ L174), R159 (= R177), D227 (= D244), H229 (= H246), H239 (≠ D256), R242 (≠ K259)
binding zinc ion: H8 (= H6), H10 (= H8), D16 (= D14), H41 (= H39), E80 (= E99), E80 (= E99), H108 (= H127), H153 (= H171), D227 (= D244), H229 (= H246)

2yz5A Histidinol phosphate phosphatase complexed with phosphate (see paper)
26% identity, 100% coverage: 2:283/283 of query aligns to 1:263/263 of 2yz5A

query
sites
2yz5A

L

M

A

V

D

D

Y

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H

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H

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L

binding fe (iii) ion: H5 (= H6), H7 (= H8), E80 (= E99), E80 (= E99), H108 (= H127), H154 (= H171), D224 (= D244)
binding phosphate ion: H7 (= H8), H38 (= H39), H226 (= H246)
binding zinc ion: H13 (≠ D14), H38 (= H39), H226 (= H246)

2yxoB Histidinol phosphate phosphatase complexed with sulfate (see paper)
26% identity, 100% coverage: 2:283/283 of query aligns to 1:263/265 of 2yxoB

query
sites
2yxoB

L

M

A

V

D

D

Y

S

H
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H

V

V

H
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H

T

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x
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W

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G

-

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binding fe (iii) ion: H5 (= H6), H7 (= H8), H13 (≠ D14), H38 (= H39), E80 (= E99), D224 (= D244), H226 (= H246)
binding sulfate ion: H7 (= H8), H38 (= H39), H108 (= H127), H226 (= H246)
binding zinc ion: E80 (= E99), H108 (= H127), H154 (= H171)

Query Sequence

>WP_005346048.1 NCBI__GCF_000173975.1:WP_005346048.1
MLADYHVHTEFSNDSIYPMEEVVKDAISLGIKDICFTDHVDYGPYRDWDDPRGIQYRPGD
EGEPEQVALTNVDYKKYFSMIEKMREKYREKIAIKAGLEFGVQTHTIPEYEKLFRSYPFD
FIILSIHQAGDQEFWTNEYQSGRTQQEYNEGYYKELLSVVQNYHNYSVLGHMDLIVRYDS
YGVYPFEKLKPLLTEILKTVIADGKGIEVNTSNHRYGLSDMTPSRDILKLYKELGGTIIT
IGSDSHKKEHLGAYIDWAKEELRKLGYTQFCTFEKMQPIFHEL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory