SitesBLAST

P10880 Shikimate kinase 2; SK 2; Shikimate kinase II; SKII; EC 2.7.1.71 from Dickeya chrysanthemi (Pectobacterium chrysanthemi) (Erwinia chrysanthemi) (see paper)
27% identity, 60% coverage: 93:249/262 of query aligns to 5:163/173 of P10880

query
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P10880

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C13 (≠ A101) mutation to S: 66% of wild-type activity. Increase in substrates affinity.
K15 (= K103) mutation to M: Loss of activity. Increased thermostability.
D34 (= D122) mutation to N: Loss of activity.
C162 (= C248) mutation to S: No effect on activity and substrates affinity.

3bafA Crystal structure of shikimate kinase from mycobacterium tuberculosis in complex with amp-pnp
28% identity, 62% coverage: 94:255/262 of query aligns to 5:165/165 of 3bafA

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3bafA

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binding phosphoaminophosphonic acid-adenylate ester: P10 (≠ M99), G13 (= G102), K14 (= K103), S15 (≠ T104), T16 (≠ S105), R109 (= R199), R116 (= R206)
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: D33 (= D122), R135 (= R225)

2iywA Shikimate kinase from mycobacterium tuberculosis in complex with mgatp, open lid (conf. B) (see paper)
28% identity, 62% coverage: 94:255/262 of query aligns to 5:165/178 of 2iywA

query
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2iywA

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binding adenosine-5'-triphosphate: G11 (= G100), G13 (= G102), K14 (= K103), S15 (≠ T104), T16 (≠ S105), R109 (= R199), R152 (≠ K242), P154 (≠ L244)

2dfnA Structure of shikimate kinase from mycobacterium tuberculosis complexed with adp and shikimate at 1.9 angstrons of resolution (see paper)
28% identity, 62% coverage: 94:255/262 of query aligns to 5:165/165 of 2dfnA

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2dfnA

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binding adenosine-5'-diphosphate: G11 (= G100), S12 (≠ A101), G13 (= G102), K14 (= K103), S15 (≠ T104), T16 (≠ S105), R109 (= R199), R116 (= R206), R152 (≠ K242), P154 (≠ L244)
binding chloride ion: P10 (≠ M99), K14 (= K103), G79 (= G168)
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: D33 (= D122), I44 (= I133), F56 (= F145), R57 (= R146), G78 (= G167), G79 (= G168), G80 (= G169), R135 (= R225)

1we2A Crystal structure of shikimate kinase from mycobacterium tuberculosis in complex with mgadp and shikimic acid (see paper)
28% identity, 62% coverage: 94:255/262 of query aligns to 5:165/165 of 1we2A

query
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1we2A

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binding adenosine-5'-diphosphate: P10 (≠ M99), G11 (= G100), G13 (= G102), K14 (= K103), S15 (≠ T104), T16 (≠ S105), R109 (= R199), R116 (= R206)
binding 3-dehydroshikimate: D33 (= D122), I44 (= I133), F48 (= F137), G78 (= G167), G79 (= G168), G80 (= G169), L118 (≠ V208), R135 (= R225)

2iyvA Shikimate kinase from mycobacterium tuberculosis in complex with adp, open lid (conf. B) (see paper)
28% identity, 62% coverage: 94:255/262 of query aligns to 5:165/179 of 2iyvA

query
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2iyvA

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binding adenosine-5'-diphosphate: G11 (= G100), G13 (= G102), K14 (= K103), S15 (≠ T104), T16 (≠ S105), R109 (= R199), R152 (≠ K242), P154 (≠ L244)

1zyuA Crystal structure of mycobacterium tuberculosis shikimate kinase in complex with shikimate and amppcp at 2.85 angstrom resolution (see paper)
28% identity, 62% coverage: 94:255/262 of query aligns to 5:165/168 of 1zyuA

query
sites
1zyuA

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L

binding phosphomethylphosphonic acid adenylate ester: P10 (≠ M99), G11 (= G100), G13 (= G102), K14 (= K103), S15 (≠ T104), T16 (≠ S105), R109 (= R199), T149 (= T239), R152 (≠ K242), P154 (≠ L244)
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: D33 (= D122), R57 (= R146), G80 (= G169), P117 (= P207), L118 (≠ V208), R135 (= R225)

4bqsA Crystal structure of mycobacterium tuberculosis shikimate kinase in complex with adp and a shikimic acid derivative. (see paper)
28% identity, 62% coverage: 94:255/262 of query aligns to 5:165/169 of 4bqsA

query
sites
4bqsA

V

V

L

L

I

V

G

G

F

L

M
x
P

G
|
G

A

S

G
|
G

K
|
K

T
x
S

S
x
T

I

I

S

G

E

R

Y

R

L

L

K

A

T

K

L

A

F

L

A

G

M

V

D

G

V

L

I

L

E

D

M

T

D
|
D

Q

V

I

A

I

I

A

E

E

Q

R

R

E

T

G

G

M

R

S

S

I

I

P

A

D

D

I

I

F

F

E

A

V

T

H

D

G

G

E

E

Q

Q

Y

E

F

F

R

R

D

R

L

I

E

E

T

E

N

D

L

V

I

R

E

A

M

A

Q

L

S

A

R

D

K

H

N

D

V

G

V

V

I

L

S

S

C

L

G

G

T

A

P

-

L

V

R

T

E

S

C

P

N

G

V

V

V

R

E

A

M

A

K

L

K

A

N

G

G

H

R

T

V

V

L

Y

L

L

T

E

A

I

S

S

P

A

E

A

T

E

I

G

F

V

D

R

R
|
R

V

T

K

G

D

G

S

N

H

T

D

V

R

R

P

P

V

L

I

L

E

A

N

G

N

P

K

D

N

R

V

A

P

E

F

K

I

Y

A

R

D

A

L

L

M

M

E

A

K

K

R
|
R

R

A

A

P

K

L

Y

Y

E

R

A

R

A

V

A

A

D

T

I

M

I

R

I

V

N

D

T

T

D

N

G

R

K
x
R

S

N

L
x
P

I

G

E

A

V

V

C

V

E

R

E

H

L

I

V

L

Q

S

Q

R

L

L

binding adenosine-5'-diphosphate: P10 (≠ M99), G11 (= G100), G13 (= G102), K14 (= K103), S15 (≠ T104), T16 (≠ S105), R109 (= R199), R152 (≠ K242), P154 (≠ L244)
binding (1R,6R,10S)-6,10-dihydroxy-2-oxabicyclo[4.3.1]deca-4(Z),7-diene-8-carboxylic acid: D33 (= D122), R135 (= R225)

2iyyA Shikimate kinase from mycobacterium tuberculosis in complex with shikimate-3-phosphate and so4 (see paper)
28% identity, 62% coverage: 94:255/262 of query aligns to 5:165/169 of 2iyyA

query
sites
2iyyA

V

V

L

L

I

V

G

G

F

L

M

P

G

G

A

S

G

G

K
|
K

T

S

S

T

I

I

S

G

E

R

Y

R

L

L

K

A

T

K

L

A

F

L

A

G

M

V

D

G

V

L

I

L

E

D

M

T

D
|
D

Q

V

I

A

I

I

A
x
E

E

Q

R

R

E

T

G

G

M

R

S
|
S

I
|
I

P

A

D

D

I

I

F

F

E

A

V

T

H

D

G

G

E

E

Q

Q

Y

E

F

F

R
|
R

D

R

L

I

E

E

T

E

N

D

L

V

I

R

E

A

M

A

Q

L

S

A

R

D

K

H

N

D

V

G

V

V

I

L

S

S

C

L

G

G

G
|
G

T

A

P

-

L

V

R

T

E

S

C

P

N

G

V

V

V

R

E

A

M

A

K

L

K

A

N

G

G

H

R

T

V

V

L

Y

L

L

T

E

A

I

S

S

P

A

E

A

T

E

I

G

F

V

D

R

R

R

V

T

K

G

D

G

S

N

H

T

D

V

R

R

P

P

V
x
L

I

L

E

A

N

G

N

P

K

D

N

R

V

A

P

E

F

K

I

Y

A

R

D

A

L

L

M

M

E

A

K

K

R
|
R

R

A

A

P

K

L

Y

Y

E

R

A

R

A

V

A

A

D

T

I

M

I

R

I

V

N

D

T

T

D

N

G

R

K

R

S

N

L

P

I

G

E

A

V

V

C

V

E

R

E

H

L

I

V

L

Q

S

Q

R

L

L

binding magnesium ion: E37 (≠ A126), S43 (= S132), I44 (= I133)
binding phosphate ion: K14 (= K103), G79 (= G168)
binding shikimate-3-phosphate: K14 (= K103), D33 (= D122), R57 (= R146), G79 (= G168), G80 (= G169), L118 (≠ V208), R135 (= R225)

1e6cA K15m mutant of shikimate kinase from erwinia chrysanthemi (see paper)
26% identity, 60% coverage: 93:249/262 of query aligns to 5:163/170 of 1e6cA

query
sites
1e6cA

I

I

V

F

L

M

I

V

G

G

F

A

M
x
R

G
|
G

A

C

G

G

K
x
M

T
|
T

S

T

I

V

S

G

E

R

Y

E

L

L

K

A

T

R

L

A

F

L

A

G

M

Y

D

E

V

F

I

V

E

D

M

T

D

D

Q

I

I

F

I

M

A

Q

E

H

R

T

E

S

G

G

M

M

S

T

I

V

P

A

D

D

I

V

F

V

E

A

V

A

H

E

G

G

E

W

Q

P

Y

G

F

F

R

R

D

R

L

R

E

E

T

S

N

E

L

A

L

L

I

-

E

Q

M

A

Q

V

S

A

R

T

K

P

N

N

V

R

V

V

I

V

S

A

C

T

G

G

T

M

P

V

L

L

R

L

E

E

C

Q

N

N

V

R

V

Q

E

F

M

M

K

R

K

A

N

H

G

G

R

T

V

V

L

Y

L

L

T

F

A

A

S

P

P

A

E

E

T

E

I

L

F

A

D

L

R

R

V

L

K

Q

D

A

S

S

-

L

-

Q

-

A

H

H

D

Q

R

R

P

P

V

T

I

L

E

T

N

G

N

R

K

P

N

I

V

A

P

E

F

E

I

M

A

E

D

A

L

V

M

L

E

R

K

E

R

R

R

E

A

A

K

L

Y

Y

E

Q

A

D

A

V

A

A

D

H

I

Y

I

V

N

D

T

A

D

T

G

Q

K

P

S

P

L

A

I

A

E

I

V

V

C

C

E

E

binding phosphate ion: R11 (≠ M99), G12 (= G100), M15 (≠ K103), T16 (= T104)

2shkB The three-dimensional structure of shikimate kinase from erwinia chrysanthemi complexed with adp (see paper)
27% identity, 60% coverage: 93:249/262 of query aligns to 5:153/162 of 2shkB

query
sites
2shkB

I

I

V

F

L

M

I

V

G

G

F

A

M

R

G
|
G

A
x
C

G
|
G

K
|
K

T
|
T

S
x
T

I

V

S

G

E

R

Y

E

L

L

K

A

T

R

L

A

F

L

A

G

M

Y

D

E

V

F

I

V

E

D

M

T

D

D

Q

I

I

F

I

M

A

Q

E

H

R

T

E

S

G

G

M

M

S

T

I

V

P

A

D

D

I

V

F

V

E

A

V

A

H

E

G

G

E

W

Q

P

Y

G

F

F

R

R

D

R

L

R

E

E

T

S

N

E

L

A

L

L

I

-

E

Q

M

A

Q

V

S

A

R

T

K

P

N

N

V

R

V

V

I

V

S

A

C

T

G

G

T

M

P

V

L

L

R

L

E

E

C

Q

N

N

V

R

V

Q

E

F

M

M

K

R

K

A

N

H

G

G

R

T

V

V

L

Y

L

L

T

F

A

A

S

P

P

A

E

E

T

E

I

L

F

A

D

L

R
|
R

V

L

K

Q

D

L

S

T

H

-

D

G

R

R

P

P

V

I

I

A

E

E

N

E

N

M

K

E

N

A

V

V

P

-

F

-

I

-

A

-

D

-

L

-

M

L

E

R

K

E

R

R

R

E

A

A

K

L

Y

Y

E
x
Q

A

D

A

V

A
|
A

D

H

I

Y

I

V

N

D

T

A

D

T

G
x
Q

K

P

S
x
P

L

A

I

A

E

I

V

V

C

C

E

E

binding adenosine-5'-diphosphate: G12 (= G100), C13 (≠ A101), G14 (= G102), K15 (= K103), T16 (= T104), T17 (≠ S105), R110 (= R199), Q145 (≠ G241), P147 (≠ S243)
binding magnesium ion: Q134 (≠ E230), A137 (= A233)

6hqvA Pentafunctional arom complex from chaetomium thermophilum (see paper)
30% identity, 52% coverage: 90:226/262 of query aligns to 842:980/1555 of 6hqvA

query
sites
6hqvA

D

D

Y

R

N

S

I

I

V

F

L

I

I

V

G

G

F

M

M

R

G

G

A

A

G

G

K

K

T

S

S

T

I

A

S

G

E

R

Y

W

L

M

K

S

T

E

L

L

F

L

A

K

M

R

D

P

V

L

I

V

E

D

M

L

D
|
D

Q

A

I

E

I

L

A

E

E

R

R

R

E

E

G

G

M

M

S

T

I

I

P

P

D

E

I

I

F

I

E

R

-

G

V

E

H

R

G

G

E

W

Q

E

Y

G

F

F

R

R

D

Q

L

A

E

E

T

L

N

E

L

L

-

Q

-

D

-

V

I

I

E

K

M

N

Q

Q

S

S

R

-

K

K

N

G

V

Y

V

I

I

F

S

S

C

C

G

G

G
|
G

T

I

P

V

L

E

R

T

E

E

C

A

N

A

-

R

-

K

-

L

V

L

V

I

E

D

M

Y

K

H

K

K

N

N

G

G

R

G

V

P

V

V

L

L

T

V

-

H

A

R

S

D

P

T

E

D

T

Q

I

V

F

V

D

E

R

Y

V

L

K

M

D

R

S

D

H

K

D

T

R

R

P

P

V

A

I

Y

E

S

N

E

N

N

K

-

N

-

V

-

P

-

F

-

I

I

A

R

D

E

L

V

M

Y

E

E

K

R

R
|
R

R

K

binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: D874 (= D122), G923 (= G168), G924 (= G169), R979 (= R225)

Sites not aligning to the query:

active site: 123, 145, 187, 243, 253, 257, 261, 264, 268, 280
binding (4S,5R)-4,5-dihydroxy-3-oxocyclohex-1-ene-1-carboxylic acid: 1060, 1062, 1181, 1224, 1232, 1242, 1243
binding glutamic acid: 139, 145, 187, 243, 257, 264, 280
binding nicotinamide-adenine-dinucleotide: 42, 44, 45, 76, 79, 107, 108, 109, 112, 132, 133, 135, 139, 140, 145, 154, 175, 176, 177, 180, 280
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: 413, 562, 563, 1277, 1279, 1323, 1327, 1348, 1368, 1526
binding zinc ion: 187, 264, 280

1u8aA Crystal structure of mycobacterium tuberculosis shikimate kinase in complex with shikimate and adp at 2.15 angstrom resolution (see paper)
26% identity, 62% coverage: 94:255/262 of query aligns to 5:163/163 of 1u8aA

query
sites
1u8aA

V

V

L

L

I

V

G

G

F

L

M

P

G
|
G

A

S

G
|
G

K
|
K

T
x
S

S
x
T

I

I

S

G

E

R

Y

R

L

L

K

A

T

K

L

A

F

L

A

G

M

V

D

G

V

L

I

L

E

D

M

T

D
|
D

Q

V

I

A

I

I

A

E

E

Q

R

R

E

T

G

G

M

R

S

S

I

I

P

A

D

D

I

I

F
|
F

E

A

V

T

H

D

G

G

E

E

Q

Q

Y

E

F

F

R
|
R

D

R

L

I

E

E

T

E

N

D

L

V

I

R

E

A

M

A

Q

L

S

A

R

D

K

H

N

D

V

G

V

V

I

L

S

S

C

L

G

G

G
|
G

T

A

P

V

L

T

R

S

E

P

C

G

N

V

V

R

V

A

E

A

M

L

K

A

K

G

N

H

G

T

R

V

V

V

V

Y

L

L

L

E

T

I

A

S

S

A

P

A

E

E

T

G

I

V

F

R

D

-

R

-

V

-

K
x
R

D

T

S

G

H

G

D

V

R

R

P

P

V
x
L

I

L

E

A

N

G

N

P

K

D

N

R

V

A

P

E

F

K

I

Y

A

R

D

A

L

L

M

M

E

A

K

K

R
|
R

R

A

A

P

K

L

Y

Y

E

R

A

R

A

V

A

A

D

T

I

M

I

R

I

V

N

D

T

T

D

N

G

R

K
x
R

S

N

L
x
P

I

G

E

A

V

V

C

V

E

R

E

H

L

I

V

L

Q

S

Q

R

L

L

binding adenosine-5'-diphosphate: G11 (= G100), G13 (= G102), K14 (= K103), S15 (≠ T104), T16 (≠ S105), R109 (≠ K201), R150 (≠ K242), P152 (≠ L244)
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: D33 (= D122), F48 (= F137), R57 (= R146), G79 (= G168), G80 (= G169), L116 (≠ V208), R133 (= R225)

P07547 Pentafunctional AROM polypeptide; EC 4.2.3.4; EC 2.5.1.19; EC 2.7.1.71; EC 4.2.1.10; EC 1.1.1.25 from Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) (see 2 papers)
27% identity, 55% coverage: 92:235/262 of query aligns to 865:1009/1583 of P07547

query
sites
P07547

N

S

I

I

V

Y

L

I

I

I

G

G

F

M

M

R

G

G

A

A

G

G

K

K

T

S

S

T

I

A

S

G

E

N

Y

W

L

V

K

S

T

K

L

A

F

L

A

N

M

R

D

P

V

F

I

V

E

D

M

L

D

D

Q

T

I

E

I

L

A

E

E

T

R

V

E

E

G

G

M

M

S

T

I

I

P

P

D

D

I

I

F

I

E

K

V

T

H

R

G

G

E

W

Q

Q

Y

G

F

F

R

R

D

N

L

A

E

E

T

L

N

E

L

I

L

L

I

K

E

R

M

T

-

L

-

K

Q

E

S

R

R

S

K

R

N

G

V

Y

V

V

I

F

S

A

C

C

G

G

T

-

P

-

L

-

R

-

E

-

C

-

N

-

V

V

E

E

M

M

-

P

-

E

-

A

-

R

-

K

-

L

-

T

-

D

-

Y

-

H

K

K

K

T

N

K

G

G

R

N

V

V

V

L

L

L

T

M

A

R

S

D

P

I

E

K

T

K

I

I

F

M

D

D

R

F

V

L

K

S

D

I

S

D

H

K

D

T

R

R

P

P

V

A

I

-

E

-

N

-

K

-

N

-

V

-

P

-

F

Y

I

V

A

E

D

D

L

M

M

M

E

G

-

V

-

W

-

L

K

R

R

R

R

K

A

P

K

W

Y

F

E

Q

A

E

A

C

A

S

D

N

I

I

Sites not aligning to the query:

44:46 binding NAD(+)
81:84 binding NAD(+)
114:116 binding NAD(+)
119 binding NAD(+)
139:140 binding NAD(+)
161 binding NAD(+)
179:182 binding NAD(+)
190 binding NAD(+)
194 binding Zn(2+)
271 binding Zn(2+)
287 binding Zn(2+)

7tbvA Crystal structure of the shikimate kinase + 3-dehydroquinate dehydratase + 3-dehydroshikimate dehydrogenase domains of aro1 from candida albicans (see paper)
32% identity, 38% coverage: 90:188/262 of query aligns to 3:106/682 of 7tbvA

query
sites
7tbvA

D

D

Y

K

N

S

I

I

V

I

L

V

I

I

G

G

F

M

M

R

G

G

A

T

G

G

K

K

T

S

S

T

I

L

S

S

E

E

Y

W

L

L

K

A

T

S

L

F

F

L

A

G

M

F

D

K

V

M

I

L

E

D

M

M

D

D

Q

K

I

Y

I

L

A

E

E

E

R

K

E

L

G

G

M

T

S

G

I

I

P

K

D

S

I

L

F

I

E

K

V

A

H

K

G

G

E

W

Q

E

Y

Y

F

F

R

R

D

Q

L

E

E

E

T

A

N

I

L

V

L

A

I

K

E

E

M

C

Q

F

S

T

R

K

-

F

-

S

K

K

N

G

V

Y

V

V

I

L

S

S

C

T

G

G

T

I

P

V

L

E

R

G

E

E

C

D

N

A

V

R

V

Q

E

Q

M

L

K

K

K

S

-

Y

-

A

-

D

N

N

G

G

R

G

V

I

V

V

L

L

Sites not aligning to the query:

binding magnesium ion: 300, 302, 329

1shkA The three-dimensional structure of shikimate kinase from erwinia chrysanthemi (see paper)
25% identity, 60% coverage: 93:249/262 of query aligns to 5:148/158 of 1shkA

query
sites
1shkA

I

I

V

F

L

M

I

V

G

G

F

A

M

R

G

G

A

C

G

G

K

K

T

T

S

T

I

V

S

G

E

R

Y

E

L

L

K

A

T

R

L

A

F

L

A

G

M

Y

D

E

V

F

I

V

E

D

M

T

D

D

Q

I

I

F

I

M

A

Q

E

H

R

T

E

S

G

G

M

M

S

T

I

V

P

A

D

D

I

V

F

V

E

A

V

A

H

E

G

G

E

W

Q

P

Y

G

F

F

R

R

D

R

L

R

E

E

T

S

N

E

L

A

L

L

I

-

E

Q

M

A

Q

V

S

A

R

T

K

P

N

N

V

R

V

V

I

V

S

A

C

T

G

G

T

M

P

V

L

L

R

L

E

E

C

Q

N

N

V

R

V

Q

E

F

M

M

K
x
R

K
x
A

N

H

G
|
G

R

T

V

V

L

Y

L

L

T

F

A

A

S

P

P

A

E

E

T

E

I

L

F

A

D

L

R

R

V

L

K

Q

D

I

S

A

H

E

D

E

R

-

P

-

V

-

I

-

E

-

N

-

K

-

N

-

V

-

P

-

F

-

I

M

A

E

D

A

L

V

M

L

E

R

K

E

R

R

R

E

A

A

K

L

Y

Y

E
x
Q

A

D

A

V

A
|
A

D

H

I

Y

I

V

N

D

T

A

D

T

G

Q

K

P

S

P

L

A

I

A

E

I

V

V

C

C

E

E

binding magnesium ion: R92 (≠ K181), A93 (≠ K182), G95 (= G184), Q129 (≠ E230), A132 (= A233)

Query Sequence

>WP_005347028.1 NCBI__GCF_000173975.1:WP_005347028.1
MTRLEKLRKRLGQCDEILLDAILMRHHIIKDIMAYKEENGLNILDPEQEERQKEWLDGRL
EDESNASEVWDIFHAIGKASKQMQARKLFDYNIVLIGFMGAGKTSISEYLKTLFAMDVIE
MDQIIAEREGMSIPDIFEVHGEQYFRDLETNLLIEMQSRKNVVISCGGGTPLRECNVVEM
KKNGRVVLLTASPETIFDRVKDSHDRPVIENNKNVPFIADLMEKRRAKYEAAADIIINTD
GKSLIEVCEELVQQLLAMDENK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory