SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_005347031.1 NCBI__GCF_000173975.1:WP_005347031.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4h3dB 1.95 angstrom crystal structure of of type i 3-dehydroquinate dehydratase (arod) from clostridium difficile with covalent modified comenic acid.
56% identity, 99% coverage: 4:253/253 of query aligns to 5:254/254 of 4h3dB

query
sites
4h3dB

V

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Q

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K

N

N

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I

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I

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active site: E87 (= E86), H144 (= H143), K171 (= K170)
binding 5-hydroxy-6-methyl-4-oxo-4H-pyran-2-carboxylic acid: H144 (= H143), K171 (= K170), M204 (= M203), R214 (= R213), F226 (= F225)

Q186A6 3-dehydroquinate dehydratase; 3-dehydroquinase; Type I DHQase; Type I dehydroquinase; DHQ1; EC 4.2.1.10 from Clostridioides difficile (strain 630) (Peptoclostridium difficile) (see paper)
56% identity, 99% coverage: 4:253/253 of query aligns to 5:254/255 of Q186A6

query
sites
Q186A6

V

V

V

Q

V

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N

N

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I

EWR 47:49 (= EWR 46:48) binding 3-dehydroquinate
R83 (= R82) binding 3-dehydroquinate
H144 (= H143) active site, Proton donor/acceptor
K171 (= K170) active site, Schiff-base intermediate with substrate
R214 (= R213) binding 3-dehydroquinate
S233 (= S232) binding 3-dehydroquinate
Q237 (= Q236) binding 3-dehydroquinate

3js3A Crystal structure of type i 3-dehydroquinate dehydratase (arod) from clostridium difficile with covalent reaction intermediate (see paper)
57% identity, 98% coverage: 4:252/253 of query aligns to 5:253/253 of 3js3A

query
sites
3js3A

V

V

V

Q

V

V

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N

N

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I

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I

G

G

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S

active site: E87 (= E86), H144 (= H143), K171 (= K170)
binding 3-amino-4,5-dihydroxy-cyclohex-1-enecarboxylate: E47 (= E46), R49 (= R48), K171 (= K170), M204 (= M203), R214 (= R213), F226 (= F225), S233 (= S232), A234 (= A233)

P58687 3-dehydroquinate dehydratase; 3-dehydroquinase; DHQD; Type I DHQase; Type I dehydroquinase; DHQ1; EC 4.2.1.10 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 4 papers)
57% identity, 99% coverage: 1:250/253 of query aligns to 1:250/252 of P58687

query
sites
P58687

M

M

N

K

P

T

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V

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R

N

D

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A

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x
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H

S21 (≠ P21) binding 3-dehydroquinate
EWR 46:48 (= EWR 46:48) binding 3-dehydroquinate
R82 (= R82) binding 3-dehydroquinate
E86 (= E86) mutation to A: Very strong reduction of the catalytic efficiency and almost the same affinity for 3-dehydroquinate.; mutation to Q: Strong reduction of the catalytic efficiency and slight increase of the affinity for 3-dehydroquinate.
H143 (= H143) active site, Proton donor/acceptor
K170 (= K170) active site, Schiff-base intermediate with substrate; mutation to M: Abolishes enzyme activity and 1.5-fold reduction of the affinity for 3-dehydroquinate.
R213 (= R213) binding 3-dehydroquinate
S232 (= S232) binding 3-dehydroquinate; mutation to A: Reduces enzyme activity 50-fold.
Q236 (= Q236) binding 3-dehydroquinate; mutation to A: Nearly abolishes enzyme activity.

4gujA 1.50 angstrom crystal structure of the salmonella enterica 3- dehydroquinate dehydratase (arod) in complex with shikimate (see paper)
57% identity, 98% coverage: 4:250/253 of query aligns to 3:249/251 of 4gujA

query
sites
4gujA

V

V

V

T

V

V

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R

N

D

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A

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D

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A

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S

S

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H

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D

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F

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K

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A

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M

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Q

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T

K

K

K

A

D

D

V

V

L

L

T

T

L

L

A

T

A

A

T

T

E

V

E

E

M

M

V

Q

N

E

N

R

Y

Y

A

A

D

D

R

R

P

P

I

I

T

T

M

M

S

S

M

M

A

S

A

K

T

T

G

G

V

V

I

I

S

S

R
|
R

V

L

C

A

G

G

E

E

V

V

F

F

G

G

S

S

A

A

L

A

T

T

F
|
F

G

G

A

A

V

V

G

K

K

K

A

A

S
|
S

A
|
A

P

P

G

G

Q
|
Q

M

I

G

S

A

V

G

A

E

D

L

L

K

R

E

T

M

V

L

L

T

T

T

I

L

L

H

H

active site: E85 (= E86), H142 (= H143), K169 (= K170)
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: E45 (= E46), R47 (= R48), R81 (= R82), H142 (= H143), R212 (= R213), F224 (= F225), S231 (= S232), A232 (= A233), Q235 (= Q236)

4guiA 1.78 angstrom crystal structure of the salmonella enterica 3- dehydroquinate dehydratase (arod) in complex with quinate (see paper)
57% identity, 98% coverage: 4:250/253 of query aligns to 3:249/251 of 4guiA

query
sites
4guiA

V

V

V

T

V

V

R

R

N

D

V

L

A

V

I

V

G

G

E

E

G

G

I

A

P

P

K

K

I

I

C

I

V

V

P

S

I

L

V

M

G

G

K

K

T

T

R

I

E

T

E

D

I

V

L

K

E

S

A

E

A

A

K

L

N

A

I

Y

L

R

P

E

I

A

G

D

A

F

D

D

V

I

V

L

E
|
E

W

W

R
|
R

V

V

D

D

W

H

Y

F

E

A

D

N

I

V

F

T

D

T

F

A

E

E

K

S

V

V

E

L

E

E

T

A

A

A

K

G

Q

A

L

I

R

R

E

E

V

I

L

I

G

T

E

D

M

K

P

P

I

L

L

L

F

F

T

T

F

F

R
|
R

T

S

S

A

K

K

E
|
E

G

G

E

E

K

Q

A

A

I

L

E

T

T

T

P

G

V

Q

Y

Y

V

I

E

D

L

L

N

N

Q

R

K

A

I

A

S

V

A

D

T

S

G

G

Y

L

V

V

D

D

L

M

V

I

D

D

M

L

E

E

A

L

F

F

T

T

G

G

D

D

A

E

V

V

K

K

A

A

V

T

I

V

E

G

E

Y

A

A

H

H

K

Q

H

H

G

N

V

V

K

A

V

V

V

I

A

M

S

S

N

N

H
|
H

D

D

F

F

D

H

K

K

T

T

P

P

A

A

K

A

S

E

D

E

I

I

I

V

Y

Q

R

R

L

L

R

R

K

K

M

M

Q

Q

E

E

L

L

G

G

A

A

D

D

I

I

P

P

K
|
K

I

I

A

A

V

V

M

M

P

P

Q

Q

N

T

K

K

K

A

D

D

V

V

L

L

T

T

L

L

A

T

A

A

T

T

E

V

E

E

M

M

V

Q

N

E

N

R

Y

Y

A

A

D

D

R

R

P

P

I

I

T

T

M

M

S

S

M
|
M

A

S

A

K

T

T

G

G

V

V

I

I

S

S

R
|
R

V

L

C

A

G

G

E

E

V

V

F

F

G

G

S

S

A

A

L

A

T

T

F
|
F

G

G

A

A

V

V

G

K

K

K

A

A

S

S

A
|
A

P

P

G

G

Q
|
Q

M

I

G

S

A

V

G

A

E

D

L

L

K

R

E

T

M

V

L

L

T

T

T

I

L

L

H

H

active site: E85 (= E86), H142 (= H143), K169 (= K170)
binding (1S,3R,4S,5R)-1,3,4,5-tetrahydroxycyclohexanecarboxylic acid: E45 (= E46), R47 (= R48), R81 (= R82), H142 (= H143), K169 (= K170), M204 (= M205), R212 (= R213), F224 (= F225), A232 (= A233), Q235 (= Q236)

4guhB 1.95 angstrom crystal structure of the salmonella enterica 3- dehydroquinate dehydratase (arod) e86a mutant in complex with dehydroshikimate (crystal form #2) (see paper)
57% identity, 99% coverage: 1:250/253 of query aligns to 14:263/265 of 4guhB

query
sites
4guhB

M

M

N

K

P

T

V

V

V

T

V

V

R

R

N

D

V

L

A

V

I

V

G

G

E

E

G

G

I

A

P

P

K

K

I

I

C

I

V

V

P

S

I

L

V

M

G

G

K

K

T

T

R

I

E

T

E

D

I

V

L

K

E

S

A

E

A

A

K

L

N

A

I

Y

L

R

P

E

I

A

G

D

A

F

D

D

V

I

V

L

E
|
E

W

W

R
|
R

V

V

D

D

W

H

Y

F

E

A

D

N

I

V

F

T

D

T

F

A

E

E

K

S

V

V

E

L

E

E

T

A

A

A

K

G

Q

A

L

I

R

R

E

E

V

I

L

I

G

T

E

D

M

K

P

P

I

L

L

L

F

F

T

T

F

F

R
|
R

T

S

S

A

K

K

E
x
A

G

G

E

E

K

Q

A

A

I

L

E

T

T

T

P

G

V

Q

Y

Y

V

I

E

D

L

L

N

N

Q

R

K

A

I

A

S

V

A

D

T

S

G

G

Y

L

V

V

D

D

L

M

V

I

D

D

M

L

E

E

A

L

F

F

T

T

G

G

D

D

A

E

V

V

K

K

A

A

V

T

I

V

E

G

E

Y

A

A

H

H

K

Q

H

H

G

N

V

V

K

A

V

V

V

I

A

M

S

S

N

N

H
|
H

D

D

F

F

D

H

K

K

T

T

P

P

A

A

K

A

S

E

D

E

I

I

I

V

Y

Q

R

R

L

L

R

R

K

K

M

M

Q

Q

E

E

L

L

G

G

A

A

D

D

I

I

P

P

K
|
K

I

I

A

A

V

V

M

M

P

P

Q

Q

N

T

K

K

K

A

D

D

V

V

L

L

T

T

L

L

A

T

A

A

T

T

E

V

E

E

M

M

V

Q

N

E

N

R

Y

Y

A

A

D

D

R

R

P

P

I

I

T

T

M
|
M

S

S

M

M

A

S

A

K

T

T

G

G

V

V

I

I

S

S

R
|
R

V

L

C

A

G

G

E

E

V

V

F

F

G

G

S

S

A

A

L

A

T

T

F
|
F

G

G

A

A

V

V

G

K

K

K

A

A

S

S

A
|
A

P

P

G

G

Q
|
Q

M

I

G

S

A

V

G

A

E

D

L

L

K

R

E

T

M

V

L

L

T

T

T

I

L

L

H

H

active site: A99 (≠ E86), H156 (= H143), K183 (= K170)
binding (4S,5R)-4,5-dihydroxy-3-oxocyclohex-1-ene-1-carboxylic acid: E59 (= E46), R61 (= R48), R95 (= R82), K183 (= K170), M216 (= M203), R226 (= R213), F238 (= F225), A246 (= A233), Q249 (= Q236)

3m7wA Crystal structure of type i 3-dehydroquinate dehydratase (arod) from salmonella typhimurium lt2 in covalent complex with dehydroquinate (see paper)
57% identity, 98% coverage: 4:250/253 of query aligns to 3:249/251 of 3m7wA

query
sites
3m7wA

V

V

V

T

V

V

R

R

N

D

V

L

A

V

I

V

G

G

E

E

G

G

I

A

P

P

K

K

I

I

C

I

V

V

P

S

I

L

V

M

G

G

K

K

T

T

R

I

E

T

E

D

I

V

L

K

E

S

A

E

A

A

K

L

N

A

I

Y

L

R

P

E

I

A

G

D

A

F

D

D

V

I

V

L

E
|
E

W

W

R
|
R

V

V

D

D

W

H

Y

F

E

A

D

N

I

V

F

T

D

T

F

A

E

E

K

S

V

V

E

L

E

E

T

A

A

A

K

G

Q

A

L

I

R

R

E

E

V

I

L

I

G

T

E

D

M

K

P

P

I

L

L

L

F

F

T

T

F

F

R

R

T

S

S

A

K

K

E
|
E

G

G

E

E

K

Q

A

A

I

L

E

T

T

T

P

G

V

Q

Y

Y

V

I

E

D

L

L

N

N

Q

R

K

A

I

A

S

V

A

D

T

S

G

G

Y

L

V

V

D

D

L

M

V

I

D

D

M

L

E

E

A

L

F

F

T

T

G

G

D

D

A

E

V

V

K

K

A

A

V

T

I

V

E

G

E

Y

A

A

H

H

K

Q

H

H

G

N

V

V

K

A

V

V

V

I

A

M

S

S

N

N

H
|
H

D

D

F

F

D

H

K

K

T

T

P

P

A

A

K

A

S

E

D

E

I

I

I

V

Y

Q

R

R

L

L

R

R

K

K

M

M

Q

Q

E

E

L

L

G

G

A

A

D

D

I

I

P

P

K
|
K

I

I

A

A

V

V

M

M

P

P

Q

Q

N

T

K

K

K

A

D

D

V

V

L

L

T

T

L

L

A

T

A

A

T

T

E

V

E

E

M

M

V

Q

N

E

N

R

Y

Y

A

A

D

D

R

R

P

P

I

I

T

T

M
|
M

S

S

M
|
M

A

S

A

K

T

T

G

G

V

V

I

I

S

S

R
|
R

V

L

C

A

G

G

E

E

V

V

F

F

G

G

S

S

A

A

L

A

T

T

F
|
F

G

G

A

A

V

V

G

K

K

K

A

A

S

S

A
|
A

P

P

G

G

Q
|
Q

M

I

G

S

A

V

G

A

E

D

L

L

K

R

E

T

M

V

L

L

T

T

T

I

L

L

H

H

active site: E85 (= E86), H142 (= H143), K169 (= K170)
binding 1,3,4-trihydroxy-5-oxo-cyclohexanecarboxylic acid: E45 (= E46), R47 (= R48), H142 (= H143), K169 (= K170), M202 (= M203), M204 (= M205), R212 (= R213), F224 (= F225), A232 (= A233), Q235 (= Q236)

P05194 3-dehydroquinate dehydratase; 3-dehydroquinase; Type I DHQase; Type I dehydroquinase; DHQ1; EC 4.2.1.10 from Escherichia coli (strain K12) (see 3 papers)
57% identity, 99% coverage: 1:250/253 of query aligns to 1:250/252 of P05194

query
sites
P05194

M

M

N

K

P

T

V

V

V

T

V

V

R

K

N

D

V

L

A

V

I

I

G

G

E

T

G

G

I

A

P

P

K

K

I

I

C

I

V

V

P

S

I

L

V

M

G

A

K

K

T

D

R

I

E

A

E

S

I

V

L

K

E

S

A

E

A

A

K

L

N

A

I

Y

L

R

P

E

I

A

G

D

A

F

D

D

V

I

V

L

E

E

W

W

R

R

V

V

D

D

W

H

Y

Y

E

A

D

D

I

L

F

S

D

N

F

V

E

E

K

S

V

V

E

M

E

A

T

A

A

A

K

K

Q

I

L

L

R

R

E

E

V

T

L

M

G

P

E

E

M

K

P

P

I

L

L

L

F

F

T

T

F

F

R

R

T

S

S

A

K

K

E

E

G

G

E

E

K

Q

A

A

I

I

E

S

T

T

P

E

V

A

Y

Y

V

I

E

A

L

L

N

N

Q

R

K

A

I

A

S

I

A

D

T

S

G

G

Y

L

V

V

D

D

L

M

V

I

D

D

M

L

E

E

A

L

F

F

T

T

G

G

D

D

A

Q

V

V

K

K

A

E

V

T

I

V

E

A

E

Y

A

A

H

H

K

A

H

H

G

D

V

V

K

K

V

V

A

M

S

S

N

N

H
|
H

D

D

F

F

D
x
H

K

K

T

T

P

P

A

E

K

A

S

E

D

E

I

I

Y

A

R

R

L

L

R

R

K

K

M

M

Q

Q

E

S

L

F

G

D

A

A

D

D

I

I

P

P

K
|
K

I

I

A

A

V

L

M

M

P

P

Q

Q

N

S

K

T

K

S

D

D

V

V

L

L

T

T

L

L

A

A

T

T

E

L

E

E

M

M

V

Q

N

E

N

Q

Y

Y

A

A

D

D

R

R

P

P

I

I

T

T

M

M

S

S

M
|
M

A

A

A

K

T

T

G

G

V

V

I

I

S

S

R

R

V

L

C

A

G

G

E

E

V

V

F

F

G

G

S

S

A

A

L

A

T

T

F

F

G

G

A

A

V

V

G

K

K

K

A

A

S

S

A

A

P

P

G

G

Q

Q

M

I

G

S

A

V

G

N

E

D

L

L

K

R

E

T

M

V

L

L

T

T

T

I

L

L

H

H

H143 (= H143) active site, Proton donor/acceptor; mutation to A: Loss of dehydratase activity.
H146 (≠ D146) mutation to A: It retains full catalytic activity.
K170 (= K170) active site, Schiff-base intermediate with substrate; mutation to A: Loss of dehydratase activity, but it is still able to bind substrate.
M205 (= M205) mutation to L: It has little effect on the catalytic efficiency and affinity for 3-dehydroquinate.

8b2cAAA 3-dehydroquinate dehydratase (see paper)
54% identity, 99% coverage: 1:250/253 of query aligns to 1:250/252 of 8b2cAAA

query
sites
8b2cAAA

M

M

N

K

P

T

V

V

V

T

V

V

R

K

N

N

V

L

A

I

I

I

G

G

E

E

G

G

I

M

P

P

K

K

I

I

C

I

V

V

P

S

I

L

V

M

G

G

K

R

T

D

R

I

E

N

E

S

I

V

L

K

E

A

A

E

A

A

K

L

N

A

I

Y

L

R

P

E

I

A

G

T

A

F

D

D

V

I

V

L

E
|
E

W

W

R
|
R

V

V

D

D

W

H

Y

F

E

M

D

D

I

I

F

A

D

S

F

T

E

Q

K

S

V

V

E

L

E

T

T

A

A

A

K

R

Q

V

L

I

R

R

E

D

V

A

L

M

G

P

E

D

M

I

P

P

I

L

L

L

F

F

T

T

F

F

R

R

T

S

S

A

K

K

E

E

G

G

E

E

K

Q

A

T

I

I

E

T

T

T

P

Q

V

H

Y

Y

V

L

E

T

L

L

N

N

Q

R

K

A

I

A

S

I

A

D

T

S

G

G

Y

L

V

V

D

D

L

M

V

I

D

D

M

L

E

E

A

L

F

F

T

T

G

G

D

D

D

A

A

D

V

V

K

K

A

A

V

T

I

V

E

D

E

Y

A

A

H

H

K

A

H

H

G

N

V

V

K

Y

V

V

A

M

S

S

N

N

H
|
H

D

D

F

F

D

H

K

Q

T

T

P

P

A

S

K

A

S

E

D

E

I

M

I

V

Y

L

R

R

L

L

R

R

K

K

M

M

Q

Q

E

A

L

L

G

G

A

A

D

D

I

I

P

P

K
|
K

I

I

A

A

V

V

M

M

P

P

Q

Q

N

S

K

K

K

H

D

D

V

V

L

L

T

T

L

L

A

T

A

A

T

T

E

L

E

E

M

M

V

Q

N

Q

N

H

Y

Y

A

A

D

D

R

R

P

P

I

V

I

I

T

T

M

M

S

S

M
|
M

A

A

A

K

T

E

G

G

V

V

I

I

S

S

R
|
R

V

L

C

A

G

G

E

E

V

V

F

F

G

G

S

S

A

A

L

A

T

T

F
|
F

G

G

A

A

V

V

G

K

K

Q

A

A

S

S

A
|
A

P

P

G

G

Q
|
Q

M

I

G

A

A

V

G

N

E

D

L

L

K

R

E

S

M

V

L

L

T

M

T

I

L

L

H

H

binding (1~{S},2~{R},4~{R},5~{S},6~{S})-2,4,5-trihydroxy-7-oxabicyclo[4.1.0]heptane-2-carboxylic acid: E46 (= E46), R48 (= R48), H143 (= H143), K170 (= K170), M205 (= M205), R213 (= R213), F225 (= F225), A233 (= A233), Q236 (= Q236)

8b2bAAA 3-dehydroquinate dehydratase (see paper)
54% identity, 99% coverage: 1:250/253 of query aligns to 1:250/252 of 8b2bAAA

query
sites
8b2bAAA

M

M

N

K

P

T

V

V

V

T

V

V

R

K

N

N

V

L

A

I

I

I

G

G

E

E

G

G

I

M

P

P

K

K

I

I

C

I

V

V

P

S

I

L

V

M

G

G

K

R

T

D

R

I

E

N

E

S

I

V

L

K

E

A

A

E

A

A

K

L

N

A

I

Y

L

R

P

E

I

A

G

T

A

F

D

D

V

I

V

L

E
|
E

W

W

R
|
R

V

V

D

D

W

H

Y

F

E

M

D

D

I

I

F

A

D

S

F

T

E

Q

K

S

V

V

E

L

E

T

T

A

A

A

K

R

Q

V

L

I

R

R

E

D

V

A

L

M

G

P

E

D

M

I

P

P

I

L

L

L

F

F

T

T

F

F

R

R

T

S

S

A

K

K

E

E

G

G

E

E

K

Q

A

T

I

I

E

T

T

T

P

Q

V

H

Y

Y

V

L

E

T

L

L

N

N

Q

R

K

A

I

A

S

I

A

D

T

S

G

G

Y

L

V

V

D

D

L

M

V

I

D

D

M

L

E

E

A

L

F

F

T

T

G

G

D

D

D

A

A

D

V

V

K

K

A

A

V

T

I

V

E

D

E

Y

A

A

H

H

K

A

H

H

G

N

V

V

K

Y

V

V

A

M

S

S

N

N

H
|
H

D

D

F

F

D

H

K

Q

T

T

P

P

A

S

K

A

S

E

D

E

I

M

I

V

Y

L

R

R

L

L

R

R

K

K

M

M

Q

Q

E

A

L

L

G

G

A

A

D

D

I

I

P

P

K
|
K

I

I

A

A

V

V

M

M

P

P

Q

Q

N

S

K

K

K

H

D

D

V

V

L

L

T

T

L

L

A

T

A

A

T

T

E

L

E

E

M

M

V

Q

N

Q

N

H

Y

Y

A

A

D

D

R

R

P

P

I

V

I

I

T

T

M

M

S

S

M

M

A

A

A

K

T

E

G

G

V

V

I

I

S

S

R
|
R

V

L

C

A

G

G

E

E

V

V

F

F

G

G

S

S

A

A

L

A

T

T

F
|
F

G

G

A

A

V

V

G

K

K

Q

A

A

S
|
S

A
|
A

P

P

G

G

Q
|
Q

M

I

G

A

A

V

G

N

E

D

L

L

K

R

E

S

M

V

L

L

T

M

T

I

L

L

H

H

binding (4R,5R)-3-amino-4,5-dihydroxy-cyclohexene-1-carboxylic acid: E46 (= E46), R48 (= R48), H143 (= H143), K170 (= K170), R213 (= R213), F225 (= F225), S232 (= S232), A233 (= A233), Q236 (= Q236)

6sfeA Crystal structure of dhq1 from salmonella typhi covalently modified by compound 7 (see paper)
54% identity, 99% coverage: 1:250/253 of query aligns to 1:250/252 of 6sfeA

query
sites
6sfeA

M

M

N

K

P

T

V

V

V

T

V

V

R

K

N

N

V

L

A

I

I

I

G

G

E

E

G

G

I

M

P

P

K

K

I

I

C

I

V

V

P

S

I

L

V

M

G

G

K

R

T

D

R

I

E

N

E

S

I

V

L

K

E

A

A

E

A

A

K

L

N

A

I

Y

L

R

P

E

I

A

G

T

A

F

D

D

V

I

V

L

E
|
E

W

W

R
|
R

V

V

D

D

W

H

Y

F

E

M

D

D

I

I

F

A

D

S

F

T

E

Q

K

S

V

V

E

L

E

T

T

A

A

A

K

R

Q

V

L

I

R

R

E

D

V

A

L

M

G

P

E

D

M

I

P

P

I

L

L

L

F

F

T

T

F

F

R
|
R

T

S

S

A

K

K

E
|
E

G

G

E

E

K

Q

A

T

I

I

E

T

T

T

P

Q

V

H

Y

Y

V

L

E

T

L

L

N

N

Q

R

K

A

I

A

S

I

A

D

T

S

G

G

Y

L

V

V

D

D

L

M

V

I

D

D

M

L

E

E

A

L

F

F

T

T

G

G

D

D

D

A

A

D

V

V

K

K

A

A

V

T

I

V

E

D

E

Y

A

A

H

H

K

A

H

H

G

N

V

V

K

Y

V

V

A

M

S

S

N

N

H
|
H

D

D

F

F

D

H

K

Q

T

T

P

P

A

S

K

A

S

E

D

E

I

M

I

V

Y

L

R

R

L

L

R

R

K

K

M

M

Q

Q

E

A

L

L

G

G

A

A

D

D

I

I

P

P

K
|
K

I

I

A

A

V

V

M

M

P

P

Q

Q

N

S

K

K

K

H

D

D

V

V

L

L

T

T

L

L

A

T

A

A

T

T

E

L

E

E

M

M

V

Q

N

Q

N

H

Y

Y

A

A

D

D

R

R

P

P

I

V

I

I

T

T

M

M

S

S

M

M

A

A

A

K

T

E

G

G

V

V

I

I

S

S

R
|
R

V

L

C

A

G

G

E

E

V

V

F

F

G

G

S

S

A

A

L

A

T

T

F
|
F

G

G

A

A

V

V

G

K

K

Q

A

A

S
|
S

A
|
A

P

P

G

G

Q
|
Q

M

I

G

A

A

V

G

N

E

D

L

L

K

R

E

S

M

V

L

L

T

M

T

I

L

L

H

H

active site: E86 (= E86), H143 (= H143), K170 (= K170)
binding (1~{S},3~{S},4~{S},5~{R})-3-(aminomethyl)-3,4,5-tris(hydroxyl)cyclohexane-1-carboxylic acid: E46 (= E46), R48 (= R48), R82 (= R82), H143 (= H143), K170 (= K170), R213 (= R213), F225 (= F225), S232 (= S232), A233 (= A233), Q236 (= Q236)

6h5jA Crystal structure of dhq1 from salmonella typhi covalently modified by ligand 4
54% identity, 99% coverage: 1:250/253 of query aligns to 1:250/252 of 6h5jA

query
sites
6h5jA

M

M

N

K

P

T

V

V

V

T

V

V

R

K

N

N

V

L

A

I

I

I

G

G

E

E

G

G

I

M

P

P

K

K

I

I

C

I

V

V

P

S

I

L

V

M

G

G

K

R

T

D

R

I

E

N

E

S

I

V

L

K

E

A

A

E

A

A

K

L

N

A

I

Y

L

R

P

E

I

A

G

T

A

F

D

D

V

I

V

L

E
|
E

W

W

R
|
R

V

V

D

D

W

H

Y

F

E

M

D

D

I

I

F

A

D

S

F

T

E

Q

K

S

V

V

E

L

E

T

T

A

A

A

K

R

Q

V

L

I

R

R

E

D

V

A

L

M

G

P

E

D

M

I

P

P

I

L

L

L

F

F

T

T

F

F

R
|
R

T

S

S

A

K

K

E
|
E

G

G

E

E

K

Q

A

T

I

I

E

T

T

T

P

Q

V

H

Y

Y

V

L

E

T

L

L

N

N

Q

R

K

A

I

A

S

I

A

D

T

S

G

G

Y

L

V

V

D

D

L

M

V

I

D

D

M

L

E

E

A

L

F

F

T

T

G

G

D

D

D

A

A

D

V

V

K

K

A

A

V

T

I

V

E

D

E

Y

A

A

H

H

K

A

H

H

G

N

V

V

K

Y

V

V

A

M

S

S

N

N

H
|
H

D

D

F

F

D

H

K

Q

T

T

P

P

A

S

K

A

S

E

D

E

I

M

I

V

Y

L

R

R

L

L

R

R

K

K

M

M

Q

Q

E

A

L

L

G

G

A

A

D

D

I

I

P

P

K
|
K

I

I

A

A

V

V

M

M

P

P

Q

Q

N

S

K

K

K

H

D

D

V

V

L

L

T

T

L

L

A

T

A

A

T

T

E

L

E

E

M

M

V

Q

N

Q

N

H

Y

Y

A

A

D

D

R

R

P

P

I

V

I

I

T

T

M

M

S

S

M

M

A

A

A

K

T

E

G

G

V

V

I

I

S

S

R
|
R

V

L

C

A

G

G

E

E

V

V

F

F

G

G

S

S

A

A

L

A

T

T

F
|
F

G

G

A

A

V

V

G

K

K

Q

A

A

S
|
S

A
|
A

P

P

G

G

Q
|
Q

M

I

G

A

A

V

G

N

E

D

L

L

K

R

E

S

M

V

L

L

T

M

T

I

L

L

H

H

active site: E86 (= E86), H143 (= H143), K170 (= K170)
binding (3~{R})-3,4,5-tris(hydroxyl)cyclohexane-1-carboxylic acid: E46 (= E46), R48 (= R48), R82 (= R82), H143 (= H143), K170 (= K170), R213 (= R213), F225 (= F225), S232 (= S232), A233 (= A233), Q236 (= Q236)

6h5gA Crystal structure of dhq1 from salmonella typhi covalently modified by ligand 3
54% identity, 99% coverage: 1:250/253 of query aligns to 1:250/252 of 6h5gA

query
sites
6h5gA

M

M

N

K

P

T

V

V

V

T

V

V

R

K

N

N

V

L

A

I

I

I

G

G

E

E

G

G

I

M

P

P

K

K

I

I

C

I

V

V

P

S

I

L

V

M

G

G

K

R

T

D

R

I

E

N

E

S

I

V

L

K

E

A

A

E

A

A

K

L

N

A

I

Y

L

R

P

E

I

A

G

T

A

F

D

D

V

I

V

L

E
|
E

W

W

R
|
R

V

V

D

D

W

H

Y

F

E

M

D

D

I

I

F

A

D

S

F

T

E

Q

K

S

V

V

E

L

E

T

T

A

A

A

K

R

Q

V

L

I

R

R

E

D

V

A

L

M

G

P

E

D

M

I

P

P

I

L

L

L

F

F

T

T

F

F

R
|
R

T

S

S

A

K

K

E
|
E

G

G

E

E

K

Q

A

T

I

I

E

T

T

T

P

Q

V

H

Y

Y

V

L

E

T

L

L

N

N

Q

R

K

A

I

A

S

I

A

D

T

S

G

G

Y

L

V

V

D

D

L

M

V

I

D

D

M

L

E

E

A

L

F

F

T

T

G

G

D

D

D

A

A

D

V

V

K

K

A

A

V

T

I

V

E

D

E

Y

A

A

H

H

K

A

H

H

G

N

V

V

K

Y

V

V

A

M

S

S

N

N

H
|
H

D

D

F

F

D

H

K

Q

T

T

P

P

A

S

K

A

S

E

D

E

I

M

I

V

Y

L

R

R

L

L

R

R

K

K

M

M

Q

Q

E

A

L

L

G

G

A

A

D

D

I

I

P

P

K
|
K

I

I

A

A

V

V

M

M

P

P

Q

Q

N

S

K

K

K

H

D

D

V

V

L

L

T

T

L

L

A

T

A

A

T

T

E

L

E

E

M

M

V

Q

N

Q

N

H

Y

Y

A

A

D

D

R

R

P

P

I

V

I

I

T

T

M
|
M

S

S

M

M

A

A

A

K

T

E

G

G

V

V

I

I

S

S

R
|
R

V

L

C

A

G

G

E

E

V

V

F

F

G

G

S

S

A

A

L

A

T

T

F
|
F

G

G

A

A

V

V

G

K

K

Q

A

A

S
|
S

A
|
A

P

P

G

G

Q
|
Q

M

I

G

A

A

V

G

N

E

D

L

L

K

R

E

S

M

V

L

L

T

M

T

I

L

L

H

H

active site: E86 (= E86), H143 (= H143), K170 (= K170)
binding (1~{R},3~{S},4~{R},5~{R})-3-methyl-4,5-bis(hydroxyl)cyclohexane-1-carboxylic acid: E46 (= E46), R48 (= R48), R82 (= R82), K170 (= K170), M203 (= M203), R213 (= R213), F225 (= F225), S232 (= S232), A233 (= A233), Q236 (= Q236)

6h5cA Crystal structure of dhq1 from salmonella typhi covalently modified by ligand 1
54% identity, 99% coverage: 1:250/253 of query aligns to 1:250/252 of 6h5cA

query
sites
6h5cA

M

M

N

K

P

T

V

V

V

T

V

V

R

K

N

N

V

L

A

I

I

I

G

G

E

E

G

G

I

M

P

P

K

K

I

I

C

I

V

V

P

S

I

L

V

M

G

G

K

R

T

D

R

I

E

N

E

S

I

V

L

K

E

A

A

E

A

A

K

L

N

A

I

Y

L

R

P

E

I

A

G

T

A

F

D

D

V

I

V

L

E
|
E

W

W

R
|
R

V

V

D

D

W

H

Y

F

E

M

D

D

I

I

F

A

D

S

F

T

E

Q

K

S

V

V

E

L

E

T

T

A

A

A

K

R

Q

V

L

I

R

R

E

D

V

A

L

M

G

P

E

D

M

I

P

P

I

L

L

L

F

F

T

T

F

F

R
|
R

T

S

S

A

K

K

E
|
E

G

G

E

E

K

Q

A

T

I

I

E

T

T

T

P

Q

V

H

Y

Y

V

L

E

T

L

L

N

N

Q

R

K

A

I

A

S

I

A

D

T

S

G

G

Y

L

V

V

D

D

L

M

V

I

D

D

M

L

E

E

A

L

F

F

T

T

G

G

D

D

D

A

A

D

V

V

K

K

A

A

V

T

I

V

E

D

E

Y

A

A

H

H

K

A

H

H

G

N

V

V

K

Y

V

V

A

M

S

S

N

N

H
|
H

D

D

F

F

D

H

K

Q

T

T

P

P

A

S

K

A

S

E

D

E

I

M

I

V

Y

L

R

R

L

L

R

R

K

K

M

M

Q

Q

E

A

L

L

G

G

A

A

D

D

I

I

P

P

K
|
K

I

I

A

A

V

V

M

M

P

P

Q

Q

N

S

K

K

K

H

D

D

V

V

L

L

T

T

L

L

A

T

A

A

T

T

E

L

E

E

M

M

V

Q

N

Q

N

H

Y

Y

A

A

D

D

R

R

P

P

I

V

I

I

T

T

M

M

S

S

M

M

A

A

A

K

T

E

G

G

V

V

I

I

S

S

R
|
R

V

L

C

A

G

G

E

E

V

V

F

F

G

G

S

S

A

A

L

A

T

T

F
|
F

G

G

A

A

V

V

G

K

K

Q

A

A

S
|
S

A
|
A

P

P

G

G

Q
|
Q

M

I

G

A

A

V

G

N

E

D

L

L

K

R

E

S

M

V

L

L

T

M

T

I

L

L

H

H

active site: E86 (= E86), H143 (= H143), K170 (= K170)
binding (1~{S},3~{R},4~{S},5~{R})-3-methyl-3,4,5-tris(hydroxyl)cyclohexane-1-carboxylic Acid: E46 (= E46), R48 (= R48), R82 (= R82), H143 (= H143), K170 (= K170), R213 (= R213), F225 (= F225), S232 (= S232), A233 (= A233), Q236 (= Q236)

4cnpA Structure of the salmonella typhi type i dehydroquinase inhibited by a 3-epiquinic acid derivative
54% identity, 99% coverage: 1:250/253 of query aligns to 1:250/252 of 4cnpA

query
sites
4cnpA

M

M

N

K

P

T

V

V

V

T

V

V

R

K

N

N

V

L

A

I

I

I

G

G

E

E

G

G

I

M

P

P

K

K

I

I

C

I

V

V

P

S

I

L

V

M

G

G

K

R

T

D

R

I

E

N

E

S

I

V

L

K

E

A

A

E

A

A

K

L

N

A

I

Y

L

R

P

E

I

A

G

T

A

F

D

D

V

I

V

L

E
|
E

W

W

R
|
R

V

V

D

D

W

H

Y

F

E

M

D

D

I

I

F

A

D

S

F

T

E

Q

K

S

V

V

E

L

E

T

T

A

A

A

K

R

Q

V

L

I

R

R

E

D

V

A

L

M

G

P

E

D

M

I

P

P

I

L

L

L

F

F

T

T

F

F

R
|
R

T

S

S

A

K

K

E
|
E

G

G

E

E

K

Q

A

T

I

I

E

T

T

T

P

Q

V

H

Y

Y

V

L

E

T

L

L

N

N

Q

R

K

A

I

A

S

I

A

D

T

S

G

G

Y

L

V

V

D

D

L

M

V

I

D

D

M

L

E

E

A

L

F

F

T

T

G

G

D

D

D

A

A

D

V

V

K

K

A

A

V

T

I

V

E

D

E

Y

A

A

H

H

K

A

H

H

G

N

V

V

K

Y

V

V

A

M

S

S

N

N

H
|
H

D

D

F

F

D

H

K

Q

T

T

P

P

A

S

K

A

S

E

D

E

I

M

I

V

Y

L

R

R

L

L

R

R

K

K

M

M

Q

Q

E

A

L

L

G

G

A

A

D

D

I

I

P

P

K
|
K

I

I

A

A

V

V

M

M

P

P

Q

Q

N

S

K

K

K

H

D

D

V

V

L

L

T

T

L

L

A

T

A

A

T

T

E

L

E

E

M

M

V

Q

N

Q

N

H

Y

Y

A

A

D

D

R

R

P

P

I

V

I

I

T

T

M
|
M

S

S

M

M

A

A

A

K

T

E

G

G

V

V

I

I

S

S

R
|
R

V

L

C

A

G

G

E

E

V

V

F

F

G

G

S

S

A

A

L

A

T

T

F
|
F

G

G

A

A

V

V

G

K

K

Q

A

A

S
|
S

A
|
A

P

P

G

G

Q
|
Q

M

I

G

A

A

V

G

N

E

D

L

L

K

R

E

S

M

V

L

L

T

M

T

I

L

L

H

H

active site: E86 (= E86), H143 (= H143), K170 (= K170)
binding (2s)-2-hydroxy-3-epiquinic acid: E46 (= E46), R48 (= R48), R82 (= R82), K170 (= K170), M203 (= M203), R213 (= R213), F225 (= F225), S232 (= S232), A233 (= A233), Q236 (= Q236)

P24670 3-dehydroquinate dehydratase; 3-dehydroquinase; Type I DHQase; Type I dehydroquinase; DHQ1; EC 4.2.1.10 from Salmonella typhi (see 3 papers)
54% identity, 99% coverage: 1:250/253 of query aligns to 1:250/252 of P24670

query
sites
P24670

M

M

N

K

P

T

V

V

V

T

V

V

R

K

N

N

V

L

A

I

I

I

G

G

E

E

G

G

I

M

P

P

K

K

I

I

C

I

V

V

P
x
S

I

L

V

M

G

G

K

R

T

D

R

I

E

N

E

S

I

V

L

K

E

A

A

E

A

A

K

L

N

A

I

Y

L

R

P

E

I

A

G

T

A

F

D

D

V

I

V

L

E
|
E

W
|
W

R
|
R

V

V

D

D

W

H

Y

F

E

M

D

D

I

I

F

A

D

S

F

T

E

Q

K

S

V

V

E

L

E

T

T

A

A

A

K

R

Q

V

L

I

R

R

E

D

V

A

L

M

G

P

E

D

M

I

P

P

I

L

L

L

F

F

T

T

F

F

R
|
R

T

S

S

A

K

K

E

E

G

G

E

E

K

Q

A

T

I

I

E

T

T

T

P

Q

V

H

Y

Y

V

L

E

T

L

L

N

N

Q

R

K

A

I

A

S

I

A

D

T

S

G

G

Y

L

V

V

D

D

L

M

V

I

D

D

M

L

E

E

A

L

F

F

T

T

G

G

D

D

D

A

A

D

V

V

K

K

A

A

V

T

I

V

E

D

E

Y

A

A

H

H

K

A

H

H

G

N

V

V

K

Y

V

V

A

M

S

S

N

N

H

H

D

D

F

F

D

H

K

Q

T

T

P

P

A

S

K

A

S

E

D

E

I

M

I

V

Y

L

R

R

L

L

R

R

K

K

M

M

Q

Q

E

A

L

L

G

G

A

A

D

D

I

I

P

P

K
|
K

I

I

A

A

V

V

M

M

P

P

Q

Q

N

S

K

K

K

H

D

D

V

V

L

L

T

T

L

L

A

T

A

A

T

T

E

L

E

E

M

M

V

Q

N

Q

N

H

Y

Y

A

A

D

D

R

R

P

P

I

V

I

I

T

T

M

M

S

S

M

M

A

A

A

K

T

E

G

G

V

V

I

I

S

S

R
|
R

V

L

C

A

G

G

E

E

V

V

F

F

G

G

S

S

A

A

L

A

T

T

F

F

G

G

A

A

V

V

G

K

K

Q

A

A

S
|
S

A

A

P

P

G

G

Q
|
Q

M

I

G

A

A

V

G

N

E

D

L

L

K

R

E

S

M

V

L

L

T

M

T

I

L

L

H

H

S21 (≠ P21) binding 3-dehydroquinate
EWR 46:48 (= EWR 46:48) binding 3-dehydroquinate
R82 (= R82) binding 3-dehydroquinate
K170 (= K170) active site, Schiff-base intermediate with substrate
R213 (= R213) binding 3-dehydroquinate
S232 (= S232) binding 3-dehydroquinate
Q236 (= Q236) binding 3-dehydroquinate

1l9wA Crystal structure of 3-dehydroquinase from salmonella typhi complexed with reaction product (see paper)
54% identity, 99% coverage: 1:250/253 of query aligns to 1:250/252 of 1l9wA

query
sites
1l9wA

M

M

N

K

P

T

V

V

V

T

V

V

R

K

N

N

V

L

A

I

I

I

G

G

E

E

G

G

I

M

P

P

K

K

I

I

C

I

V

V

P

S

I

L

V

M

G

G

K

R

T

D

R

I

E

N

E

S

I

V

L

K

E

A

A

E

A

A

K

L

N

A

I

Y

L

R

P

E

I

A

G

T

A

F

D

D

V

I

V

L

E
|
E

W

W

R
|
R

V

V

D

D

W

H

Y

F

E

M

D

D

I

I

F

A

D

S

F

T

E

Q

K

S

V

V

E

L

E

T

T

A

A

A

K

R

Q

V

L

I

R

R

E

D

V

A

L

M

G

P

E

D

M

I

P

P

I

L

L

L

F

F

T

T

F

F

R
|
R

T

S

S

A

K

K

E
|
E

G

G

E

E

K

Q

A

T

I

I

E

T

T

T

P

Q

V

H

Y

Y

V

L

E

T

L

L

N

N

Q

R

K

A

I

A

S

I

A

D

T

S

G

G

Y

L

V

V

D

D

L

M

V

I

D

D

M

L

E

E

A

L

F

F

T

T

G

G

D

D

D

A

A

D

V

V

K

K

A

A

V

T

I

V

E

D

E

Y

A

A

H

H

K

A

H

H

G

N

V

V

K

Y

V

V

A

M

S

S

N

N

H
|
H

D

D

F

F

D

H

K

Q

T

T

P

P

A

S

K

A

S

E

D

E

I

M

I

V

Y

S

R

R

L

L

R

R

K

K

M

M

Q

Q

E

A

L

L

G

G

A

A

D

D

I

I

P

P

K
|
K

I

I

A

A

V

V

M

M

P

P

Q

Q

N

S

K

K

K

H

D

D

V

V

L

L

T

T

L

L

A

T

A

A

T

T

E

L

E

E

M

M

V

Q

N

Q

N

H

Y

Y

A

A

D

D

R

R

P

P

I

V

I

I

T

T

M

M

S

S

M

M

A

A

A

K

T

E

G

G

V

V

I

I

S

S

R
|
R

V

L

C

A

G

G

E

E

V

V

F

F

G

G

S

S

A

A

L

A

T

T

F
|
F

G

G

A

A

V

V

G

K

K

Q

A

A

S
|
S

A
|
A

P

P

G

G

Q
|
Q

M

I

G

A

A

V

G

N

E

D

L

L

K

R

E

S

M

V

L

L

T

M

T

I

L

L

H

H

active site: E86 (= E86), H143 (= H143), K170 (= K170)
binding 3-amino-4,5-dihydroxy-cyclohex-1-enecarboxylate: E46 (= E46), R48 (= R48), R82 (= R82), H143 (= H143), K170 (= K170), R213 (= R213), F225 (= F225), S232 (= S232), A233 (= A233), Q236 (= Q236)

4uioA Structure of the salmonella typhi type i dehydroquinase covalently inhibited by a 3-dehydroquinic acid derivative (see paper)
54% identity, 98% coverage: 4:250/253 of query aligns to 2:248/250 of 4uioA

query
sites
4uioA

V

V

V

T

V

V

R

K

N

N

V

L

A

I

I

I

G

G

E

E

G

G

I

M

P

P

K

K

I

I

C

I

V

V

P

S

I

L

V

M

G

G

K

R

T

D

R

I

E

N

E

S

I

V

L

K

E

A

A

E

A

A

K

L

N

A

I

Y

L

R

P

E

I

A

G

T

A

F

D

D

V

I

V

L

E
|
E

W

W

R
|
R

V

V

D

D

W

H

Y

F

E

M

D

D

I

I

F

A

D

S

F

T

E

Q

K

S

V

V

E

L

E

T

T

A

A

A

K

R

Q

V

L

I

R

R

E

D

V

A

L

M

G

P

E

D

M

I

P

P

I

L

L

L

F

F

T

T

F

F

R
|
R

T

S

S

A

K

K

E
|
E

G

G

E

E

K

Q

A

T

I

I

E

T

T

T

P

Q

V

H

Y

Y

V

L

E

T

L

L

N

N

Q

R

K

A

I

A

S

I

A

D

T

S

G

G

Y

L

V

V

D

D

L

M

V

I

D

D

M

L

E

E

A

L

F

F

T

T

G

G

D

D

D

A

A

D

V

V

K

K

A

A

V

T

I

V

E

D

E

Y

A

A

H

H

K

A

H

H

G

N

V

V

K

Y

V

V

A

M

S

S

N

N

H
|
H

D

D

F

F

D

H

K

Q

T

T

P

P

A

S

K

A

S

E

D

E

I

M

I

V

Y

L

R

R

L

L

R

R

K

K

M

M

Q

Q

E

A

L

L

G

G

A

A

D

D

I

I

P

P

K
|
K

I

I

A

A

V

V

M

M

P

P

Q

Q

N

S

K

K

K

H

D

D

V

V

L

L

T

T

L

L

A

T

A

A

T

T

E

L

E

E

M

M

V

Q

N

Q

N

H

Y

Y

A

A

D

D

R

R

P

P

I

V

I

I

T

T

M

M

S

S

M
|
M

A

A

A

K

T

E

G

G

V

V

I

I

S

S

R
|
R

V

L

C

A

G

G

E

E

V

V

F

F

G

G

S

S

A

A

L

A

T

T

F
|
F

G

G

A

A

V

V

G

K

K

Q

A

A

S

S

A
|
A

P

P

G

G

Q
|
Q

M

I

G

A

A

V

G

N

E

D

L

L

K

R

E

S

M

V

L

L

T

M

T

I

L

L

H

H

active site: E84 (= E86), H141 (= H143), K168 (= K170)
binding (1~{R},3~{R},4~{S},5~{R})-3-methyl-1,3,4,5-tetrakis(oxidanyl)cyclohexane-1-carboxylic acid: E44 (= E46), R46 (= R48), R80 (= R82), H141 (= H143), K168 (= K170), M203 (= M205), R211 (= R213), F223 (= F225), A231 (= A233), Q234 (= Q236)

4clmB Structure of salmonella typhi type i dehydroquinase irreversibly inhibited with a 1,3,4-trihydroxyciclohexane-1-carboxylic acid derivative (see paper)
54% identity, 98% coverage: 4:250/253 of query aligns to 3:249/251 of 4clmB

query
sites
4clmB

V

V

V

T

V

V

R

K

N

N

V

L

A

I

I

I

G

G

E

E

G

G

I

M

P

P

K

K

I

I

C

I

V

V

P

S

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active site: E85 (= E86), H142 (= H143), K169 (= K170)
binding (1~{S},3~{S},4~{R},5~{R})-3-methyl-1,4,5-tris(hydroxyl)cyclohexane-1-carboxylic acid: E45 (= E46), R47 (= R48), R81 (= R82), K169 (= K170), M204 (= M205), R212 (= R213), F224 (= F225), S231 (= S232), A232 (= A233), Q235 (= Q236)

Query Sequence

>WP_005347031.1 NCBI__GCF_000173975.1:WP_005347031.1
MNPVVVRNVAIGEGIPKICVPIVGKTREEILEAAKNILPIGADVVEWRVDWYEDIFDFEK
VEETAKQLREVLGEMPILFTFRTSKEGGEKAIETPVYVELNQKISATGYVDLVDMEAFTG
DDAVKAVIEEAHKHGVKVVASNHDFDKTPAKSDIIYRLRKMQELGADIPKIAVMPQNKKD
VLTLLAATEEMVNNYADRPIITMSMAATGVISRVCGEVFGSALTFGAVGKASAPGQMGAG
ELKEMLTTLHASL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory