SitesBLAST
Comparing WP_005348665.1 NCBI__GCF_000173975.1:WP_005348665.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
3uyyB Crystal structures of branched-chain aminotransferase from deinococcus radiodurans complexes with alpha-ketoisocaproate and l-glutamate suggest its radio-resistance for catalysis (see paper)
53% identity, 97% coverage: 5:340/346 of query aligns to 2:337/337 of 3uyyB
- active site: K181 (= K184)
- binding pyridoxal-5'-phosphate: R79 (= R82), R171 (= R174), K181 (= K184), Y186 (= Y189), E217 (= E220), G219 (= G222), A220 (≠ G223), A221 (= A224), S244 (= S246), I245 (= I247), T246 (= T248), G282 (= G284), T283 (= T285)
3uzoA Crystal structures of branched-chain aminotransferase from deinococcus radiodurans complexes with alpha-ketoisocaproate and l-glutamate suggest its radio-resistance for catalysis (see paper)
53% identity, 97% coverage: 6:340/346 of query aligns to 1:335/335 of 3uzoA
- active site: K179 (= K184)
- binding glutamic acid: Y48 (= Y53), F53 (= F58), R122 (= R127), V134 (= V139), Y152 (= Y157), T281 (= T285), A282 (= A286)
- binding pyridoxal-5'-phosphate: R77 (= R82), K179 (= K184), Y184 (= Y189), E215 (= E220), G217 (= G222), A218 (≠ G223), A219 (= A224), N220 (= N225), L240 (= L244), S242 (= S246), I243 (= I247), T244 (= T248), G280 (= G284), T281 (= T285)
3uzbA Crystal structures of branched-chain aminotransferase from deinococcus radiodurans complexes with alpha-ketoisocaproate and l-glutamate suggest its radio-resistance for catalysis (see paper)
53% identity, 97% coverage: 6:340/346 of query aligns to 1:335/335 of 3uzbA
- active site: K179 (= K184)
- binding 2-oxo-4-methylpentanoic acid: Y120 (= Y125), K179 (= K184), T281 (= T285), A282 (= A286)
- binding pyridoxal-5'-phosphate: R77 (= R82), K179 (= K184), Y184 (= Y189), E215 (= E220), G217 (= G222), A218 (≠ G223), A219 (= A224), N220 (= N225), S242 (= S246), I243 (= I247), T244 (= T248), G280 (= G284), T281 (= T285)
6jifB Crystal structures of branched-chain aminotransferase from pseudomonas sp. Uw4 (see paper)
51% identity, 96% coverage: 6:337/346 of query aligns to 1:331/334 of 6jifB
- binding pyridoxal-5'-phosphate: R77 (= R82), R169 (= R174), K179 (= K184), Y184 (= Y189), E215 (= E220), S218 (≠ G223), A219 (= A224), N220 (= N225), L239 (= L244), G241 (≠ S246), I242 (= I247), T243 (= T248), G279 (= G284), T280 (= T285)
3ht5A Crystal structure of ilve a branched chain amino acid transaminase from mycobacterium tuberculosis (see paper)
40% identity, 90% coverage: 31:340/346 of query aligns to 17:334/335 of 3ht5A
- active site: K171 (= K184)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: R68 (= R82), K171 (= K184), Y176 (= Y189), E207 (= E220), G210 (= G223), M211 (≠ A224), N212 (= N225), L235 (= L244), G237 (≠ S246), I238 (= I247), T239 (= T248), T281 (= T285)
3dtgA Structural analysis of mycobacterial branched chain aminotransferase- implications for inhibitor design
40% identity, 90% coverage: 28:340/346 of query aligns to 42:362/363 of 3dtgA
- active site: K199 (= K184)
- binding O-benzylhydroxylamine: F72 (= F58), Y171 (= Y157), Y204 (= Y189)
- binding pyridoxal-5'-phosphate: R96 (= R82), R189 (= R174), K199 (= K184), Y204 (= Y189), E235 (= E220), G238 (= G223), L263 (= L244), I266 (= I247), T267 (= T248), T309 (= T285)
3dtfA Structural analysis of mycobacterial branched chain aminotransferase- implications for inhibitor design (see paper)
40% identity, 90% coverage: 28:340/346 of query aligns to 42:362/363 of 3dtfA
A0R066 Branched-chain-amino-acid aminotransferase; BCAT; EC 2.6.1.42 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see 2 papers)
40% identity, 90% coverage: 28:340/346 of query aligns to 47:367/368 of A0R066
- R101 (= R82) binding pyridoxal 5'-phosphate
- Y209 (= Y189) binding pyridoxal 5'-phosphate
- K299 (≠ D276) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)
- T314 (= T285) binding pyridoxal 5'-phosphate
5u3fA Structure of mycobacterium tuberculosis ilve, a branched-chain amino acid transaminase, in complex with d-cycloserine derivative (see paper)
39% identity, 90% coverage: 31:340/346 of query aligns to 16:325/326 of 5u3fA
- active site: K162 (= K184)
- binding (5-hydroxy-6-methyl-4-{[(3-oxo-2,3-dihydro-1,2-oxazol-4-yl)amino]methyl}pyridin-3-yl)methyl dihydrogen phosphate: G45 (= G60), R67 (= R82), Y110 (= Y125), K162 (= K184), Y167 (= Y189), E198 (= E220), G201 (= G223), M202 (≠ A224), N203 (= N225), L226 (= L244), G228 (≠ S246), I229 (= I247), T230 (= T248), T272 (= T285)
7nyaA Crystal structure of human cytosolic branched-chain aminotransferase (bcat1) in complex with plp and small molecule inhibitor bay-069 (compound 36) (see paper)
32% identity, 87% coverage: 31:331/346 of query aligns to 46:356/364 of 7nyaA
- binding pyridoxal-5'-phosphate: R97 (= R82), R190 (= R174), K200 (= K184), Y205 (= Y189), E235 (= E220), T238 (≠ G223), M239 (≠ A224), L264 (= L244), G266 (≠ S246), V267 (≠ I247), T268 (= T248), G310 (= G284), T311 (= T285)
- binding 3-[4-chloranyl-3-(2-methylphenoxy)naphthalen-1-yl]-6-(trifluoromethyl)-5~{H}-pyrimidine-2,4-dione: Y68 (= Y53), F73 (= F58), Y139 (= Y125), G152 (= G138), V153 (= V139), Y171 (= Y157), Q212 (≠ I196), Q222 (≠ E206), T238 (≠ G223), A312 (= A286)
Sites not aligning to the query:
7ny9A Crystal structure of human cytosolic branched-chain aminotransferase (bcat1) in complex with plp and small molecule inhibitor compound 38 (see paper)
32% identity, 87% coverage: 31:331/346 of query aligns to 46:356/364 of 7ny9A
- binding pyridoxal-5'-phosphate: R97 (= R82), R190 (= R174), K200 (= K184), Y205 (= Y189), E235 (= E220), T238 (≠ G223), M239 (≠ A224), N240 (= N225), L264 (= L244), G266 (≠ S246), V267 (≠ I247), T268 (= T248), T311 (= T285)
- binding 3-[2,6-bis(oxidanylidene)-4-(trifluoromethyl)-5~{H}-pyrimidin-1-yl]-~{N}-methyl-2-pyridin-2-yl-imidazo[1,2-a]pyridine-7-carboxamide: Y139 (= Y125), G152 (= G138), V153 (= V139), Y171 (= Y157), Q212 (≠ I196), Q221 (≠ D205), Q222 (≠ E206), V223 (≠ N207), T238 (≠ G223)
Sites not aligning to the query:
7ny2A Crystal structure of human cytosolic branched-chain aminotransferase (bcat1) in complex with plp and small molecule inhibitor compound 35 (see paper)
32% identity, 87% coverage: 31:331/346 of query aligns to 46:356/364 of 7ny2A
- binding pyridoxal-5'-phosphate: R97 (= R82), K200 (= K184), Y205 (= Y189), E235 (= E220), T238 (≠ G223), M239 (≠ A224), L264 (= L244), G266 (≠ S246), V267 (≠ I247), T268 (= T248), T311 (= T285)
- binding 4-[2,4-bis(oxidanylidene)-6-(trifluoromethyl)-1H-pyrimidin-3-yl]-2-(2-methylphenoxy)naphthalene-1-carbonitrile: Y139 (= Y125), G152 (= G138), V153 (= V139), Y171 (= Y157), Q212 (≠ I196), Q222 (≠ E206), A312 (= A286)
Sites not aligning to the query:
7nwmA Crystal structure of human cytosolic branched-chain aminotransferase (bcat1) in complex with plp and inhibitor compound 12 (see paper)
32% identity, 87% coverage: 31:331/346 of query aligns to 47:357/365 of 7nwmA
- binding pyridoxal-5'-phosphate: R98 (= R82), R191 (= R174), K201 (= K184), Y206 (= Y189), E236 (= E220), T239 (≠ G223), M240 (≠ A224), L265 (= L244), G267 (≠ S246), V268 (≠ I247), T269 (= T248), G311 (= G284), T312 (= T285)
- binding 4-[2,4-bis(oxidanylidene)-6-(trifluoromethyl)-1H-pyrimidin-3-yl]-5-methoxy-2-(2-methylphenoxy)benzenecarbonitrile: Y69 (= Y53), F74 (= F58), Y140 (= Y125), G153 (= G138), V154 (= V139), Y172 (= Y157), Q213 (≠ I196), Q223 (≠ E206), T239 (≠ G223), A313 (= A286)
Sites not aligning to the query:
7nweA Crystal structure of human cytosolic branched-chain aminotransferase (bcat1) in complex with plp and inhibitor compound 10 (see paper)
32% identity, 87% coverage: 31:331/346 of query aligns to 47:357/365 of 7nweA
- binding pyridoxal-5'-phosphate: R98 (= R82), K201 (= K184), Y206 (= Y189), E236 (= E220), T239 (≠ G223), M240 (≠ A224), L265 (= L244), G267 (≠ S246), V268 (≠ I247), T269 (= T248), G311 (= G284), T312 (= T285)
- binding 4-[2,4-bis(oxidanylidene)-6-(trifluoromethyl)-1H-pyrimidin-3-yl]-5-methyl-2-(2-methylphenoxy)benzenecarbonitrile: Y69 (= Y53), F74 (= F58), Y140 (= Y125), G153 (= G138), V154 (= V139), Y172 (= Y157), Q213 (≠ I196), Q223 (≠ E206), T239 (≠ G223), A313 (= A286)
Sites not aligning to the query:
7nwcA Crystal structure of human cytosolic branched-chain aminotransferase (bcat1) in complex with plp and inhibitor compound 2 (see paper)
32% identity, 87% coverage: 31:331/346 of query aligns to 47:357/365 of 7nwcA
- binding pyridoxal-5'-phosphate: R98 (= R82), R191 (= R174), K201 (= K184), Y206 (= Y189), E236 (= E220), T239 (≠ G223), M240 (≠ A224), N241 (= N225), L265 (= L244), G267 (≠ S246), V268 (≠ I247), T269 (= T248), G311 (= G284), T312 (= T285)
- binding 3-(5-chloranyl-2,4-dimethoxy-phenyl)-6-(trifluoromethyl)-1H-pyrimidine-2,4-dione: Y172 (= Y157), Q223 (≠ E206), T239 (≠ G223), A313 (= A286)
Sites not aligning to the query:
7nwbA Crystal structure of human cytosolic branched-chain aminotransferase (bcat1) in complex with plp and inhibitor compound 1 (see paper)
32% identity, 87% coverage: 31:331/346 of query aligns to 47:357/365 of 7nwbA
- binding pyridoxal-5'-phosphate: R98 (= R82), R191 (= R174), K201 (= K184), Y206 (= Y189), E236 (= E220), T239 (≠ G223), M240 (≠ A224), L265 (= L244), G267 (≠ S246), V268 (≠ I247), T269 (= T248), G311 (= G284), T312 (= T285)
- binding 4-[2,4-bis(oxidanylidene)-6-(trifluoromethyl)-1H-pyrimidin-3-yl]-5-fluoranyl-2-(2-methylphenoxy)benzenecarbonitrile: Y69 (= Y53), F74 (= F58), Y140 (= Y125), G153 (= G138), V154 (= V139), Y172 (= Y157), Q213 (≠ I196), Q223 (≠ E206), A313 (= A286)
Sites not aligning to the query:
7nwaA Crystal structure of human cytosolic branched-chain aminotransferase (bcat1) in complex with plp and compound a (see paper)
32% identity, 87% coverage: 31:331/346 of query aligns to 47:357/365 of 7nwaA
- binding pyridoxal-5'-phosphate: R98 (= R82), K201 (= K184), Y206 (= Y189), E236 (= E220), T239 (≠ G223), N241 (= N225), L265 (= L244), G267 (≠ S246), V268 (≠ I247), T269 (= T248), T312 (= T285)
- binding 2-[[4-chloranyl-2,6-bis(fluoranyl)phenyl]methylamino]-7-oxidanylidene-5-propyl-4H-pyrazolo[1,5-a]pyrimidine-3-carbonitrile: R142 (= R127), G153 (= G138), V154 (= V139), Y172 (= Y157), F173 (= F158), V181 (≠ I165), Q223 (≠ E206), V237 (≠ T221), T239 (≠ G223), M240 (≠ A224), G311 (= G284), A313 (= A286), C317 (≠ S290)
2abjA Crystal structure of human branched chain amino acid transaminase in a complex with an inhibitor, c16h10n2o4f3scl, and pyridoxal 5' phosphate. (see paper)
31% identity, 87% coverage: 31:332/346 of query aligns to 48:359/366 of 2abjA
- active site: K202 (= K184)
- binding n'-(5-chlorobenzofuran-2-carbonyl)-2-(trifluoromethyl)benzenesulfonohydrazide: F75 (= F58), Y141 (= Y125), Y173 (= Y157), K202 (= K184), Q224 (≠ E206), T240 (≠ G223), M241 (≠ A224), G312 (= G284), A314 (= A286), C315 (≠ A287)
- binding pyridoxal-5'-phosphate: R99 (= R82), R192 (= R174), K202 (= K184), Y207 (= Y189), E237 (= E220), T240 (≠ G223), M241 (≠ A224), N242 (= N225), L266 (= L244), G268 (≠ S246), V269 (≠ I247), T270 (= T248), T313 (= T285)
Sites not aligning to the query:
7nxoA Crystal structure of human cytosolic branched-chain aminotransferase (bcat1) in complex with plp and small molecule inhibitor compound 24(5-f) (see paper)
32% identity, 87% coverage: 31:331/346 of query aligns to 46:353/361 of 7nxoA
- binding pyridoxal-5'-phosphate: R97 (= R82), K197 (= K184), Y202 (= Y189), E232 (= E220), T235 (≠ G223), M236 (≠ A224), N237 (= N225), L261 (= L244), G263 (≠ S246), V264 (≠ I247), T265 (= T248), T308 (= T285)
- binding 4-[2,4-bis(oxidanylidene)-6-(phenylsulfonyl)-1H-pyrimidin-3-yl]-5-fluoranyl-2-(2-methylphenoxy)benzenecarbonitrile: Y68 (= Y53), F73 (= F58), G152 (= G138), V153 (= V139), Y171 (= Y157), F172 (= F158), Q209 (≠ I196), Q218 (≠ D205), Q219 (≠ E206), T235 (≠ G223)
Sites not aligning to the query:
7nxnA Crystal structure of human cytosolic branched-chain aminotransferase (bcat1) in complex with plp and small molecule inhibitor compound 21(5-f) (see paper)
32% identity, 87% coverage: 31:331/346 of query aligns to 47:354/362 of 7nxnA
- binding pyridoxal-5'-phosphate: R98 (= R82), R188 (= R174), K198 (= K184), Y203 (= Y189), E233 (= E220), T236 (≠ G223), M237 (≠ A224), N238 (= N225), L262 (= L244), G264 (≠ S246), V265 (≠ I247), T266 (= T248), T309 (= T285)
- binding 4-[6-[bis(fluoranyl)-phenyl-methyl]-2,4-bis(oxidanylidene)-1~{H}-pyrimidin-3-yl]-5-fluoranyl-2-(2-methylphenoxy)benzenecarbonitrile: Y69 (= Y53), F74 (= F58), G153 (= G138), V154 (= V139), Y172 (= Y157), Q210 (≠ I196), Q219 (≠ D205), Q220 (≠ E206), T236 (≠ G223)
Sites not aligning to the query:
Query Sequence
>WP_005348665.1 NCBI__GCF_000173975.1:WP_005348665.1
MEKKNINWGELGFSYQQTEKRFVANFKDGAWDDGTLTEDATITLNECAGVFQYAQTCFEG
LKAYTTENGDIVCFRPDLNAARMADSCRRLEMPVFPEDKFVEAVVETVKANAGYVAPYGS
GATLYIRPYMFGSNPVIGVKPADEYQFRIFVTPVGPYFKGGAKPITIRVSDFDRAAPNGT
GHIKAGLNYAMSLHAIVDAHNQGYDENMYLDPATRTKVEETGGANFIFITKDGKFVTPKS
DSILPSITRRSLMYIAEHYLGMEVEHREVRFDEVKDFAECGLCGTAAVISPVGKIVDHGK
EICFPSGMEEMGPVTKKLYDTLTGIQMGHIEAPEGWIKTICKHGEC
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory