SitesBLAST
Comparing WP_005350757.1 NCBI__GCF_000173975.1:WP_005350757.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
6dzsA Mycobacterial homoserine dehydrogenase thra in complex with NADP
45% identity, 99% coverage: 6:429/430 of query aligns to 2:428/431 of 6dzsA
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G11 (= G15), L12 (≠ A16), G13 (= G17), N14 (≠ T18), V15 (= V19), V45 (= V49), R46 (= R50), R47 (≠ D51), R52 (= R56), I63 (≠ W68), L78 (≠ V83), M79 (= M84), P84 (= P89), A102 (= A107), K104 (= K109), G306 (= G306), T310 (= T310)
Q5F8J4 Homoserine dehydrogenase; HDH; HSD; NAD(+)-dependent homoserine dehydrogenase; NAD(+)-dependent HSD; NgHSD; EC 1.1.1.3 from Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090) (see paper)
39% identity, 94% coverage: 27:429/430 of query aligns to 22:430/435 of Q5F8J4
- L45 (≠ R50) mutation to R: Shows a marked increase in the catalytic efficiency with NADP(+).
- LS 45:46 (≠ RD 50:51) mutation to RD: Does not impair the catalytic activity with NAD(+). Slightly increases the activity, but slightly decreases the affinity for NADP(+).; mutation to RR: Causes a shift in coenzyme preference from NAD(+) to NADP(+) by a factor of 974. Shows a slight decrease in the catalytic efficiency with NAD(+) and a 4.5-fold increase in catalytic efficiency with NADP(+).
4pg7A Crystal structure of s. Aureus homoserine dehydrogenase at ph7.5 (see paper)
45% identity, 73% coverage: 7:322/430 of query aligns to 3:307/402 of 4pg7A
Sites not aligning to the query:
6a0tB Homoserine dehydrogenase k99a mutant from thermus thermophilus hb8 complexed with hse and NADP+ (see paper)
39% identity, 66% coverage: 9:291/430 of query aligns to 4:275/332 of 6a0tB
- active site: D191 (= D205), K195 (= K209)
- binding l-homoserine: N150 (= N163), G151 (= G164), T152 (= T165), Y178 (= Y192), E180 (= E194), D186 (= D200), K195 (= K209)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G10 (= G15), G11 (≠ A16), G12 (= G17), T13 (= T18), V14 (= V19), L42 (= L48), V43 (= V49), R44 (= R50), D45 (= D51), K48 (= K54), R50 (= R56), A73 (≠ V83), M74 (= M84), G75 (= G85), A97 (= A107), N98 (= N108), G177 (= G191), E180 (= E194)
Sites not aligning to the query:
6a0sA Homoserine dehydrogenase from thermus thermophilus hb8 complexed with hse and NADPH (see paper)
39% identity, 66% coverage: 9:291/430 of query aligns to 4:275/331 of 6a0sA
- active site: D191 (= D205), K195 (= K209)
- binding l-homoserine: K99 (= K109), N150 (= N163), G151 (= G164), T152 (= T165), Y178 (= Y192), E180 (= E194), D186 (= D200), K195 (= K209)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G11 (≠ A16), G12 (= G17), T13 (= T18), V14 (= V19), L42 (= L48), V43 (= V49), R44 (= R50), D45 (= D51), K48 (= K54), R50 (= R56), A73 (≠ V83), M74 (= M84), A97 (= A107), K99 (= K109), G177 (= G191), E180 (= E194)
Sites not aligning to the query:
2ejwA Homoserine dehydrogenase from thermus thermophilus hb8
39% identity, 66% coverage: 9:291/430 of query aligns to 4:275/331 of 2ejwA
F9VNG5 Homoserine dehydrogenase; HDH; HSD; StHSD; EC 1.1.1.3 from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see 3 papers)
34% identity, 73% coverage: 9:323/430 of query aligns to 1:301/304 of F9VNG5
- Y8 (≠ A16) binding NADP(+)
- N10 (≠ T18) binding NADP(+)
- V11 (= V19) binding NADP(+)
- R38 (≠ I44) binding NADP(+)
- R39 (≠ T45) binding NADP(+)
- S73 (vs. gap) binding NADP(+)
- T100 (≠ A107) binding NADP(+)
- K102 (= K109) binding NADP(+)
- G182 (= G191) binding NADP(+)
- E185 (= E194) binding NADP(+)
- G284 (= G306) binding NADP(+)
Sites not aligning to the query:
7f4cA The crystal structure of the immature holo-enzyme of homoserine dehydrogenase complexed with NADP and 1,4-butandiol from the hyperthermophilic archaeon sulfurisphaera tokodaii. (see paper)
33% identity, 73% coverage: 9:323/430 of query aligns to 1:297/300 of 7f4cA
- binding nadp nicotinamide-adenine-dinucleotide phosphate: F6 (≠ L14), G7 (= G15), Y8 (≠ A16), G9 (= G17), N10 (≠ T18), V11 (= V19), T37 (≠ Q43), R38 (≠ I44), R39 (≠ T45), V72 (= V83), S73 (vs. gap), S74 (vs. gap), T100 (≠ A107), K102 (= K109), G127 (≠ A134), S131 (≠ G138), E185 (= E194), G280 (= G306), A284 (≠ T310)
5x9dA Crystal structure of homoserine dehydrogenase in complex with l- cysteine and NAD (see paper)
35% identity, 73% coverage: 9:323/430 of query aligns to 1:299/302 of 5x9dA
- active site: D196 (= D205), K200 (= K209)
- binding (2R)-3-[[(4S)-3-aminocarbonyl-1-[(2R,3R,4S,5R)-5-[[[[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxymethyl]-3,4-bis(oxidanyl)oxolan-2-yl]-4H-pyridin-4-yl]sulfanyl]-2-azanyl-propanoic acid: F6 (≠ L14), G7 (= G15), Y8 (≠ A16), G9 (= G17), N10 (≠ T18), V11 (= V19), T37 (≠ Q43), R38 (≠ I44), V72 (= V83), S73 (vs. gap), S74 (vs. gap), P82 (= P89), T100 (≠ A107), N101 (= N108), K102 (= K109), G127 (≠ A134), S131 (≠ G138), N155 (= N163), G156 (= G164), T157 (= T165), Y183 (= Y192), A184 (= A193), E185 (= E194), D191 (= D200), D196 (= D205), K200 (= K209), A281 (= A305), G282 (= G306), A286 (≠ T310)
4xb1A Hyperthermophilic archaeal homoserine dehydrogenase in complex with NADPH (see paper)
28% identity, 72% coverage: 9:319/430 of query aligns to 3:309/319 of 4xb1A
- active site: D211 (= D205), K215 (= K209)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: F8 (≠ L14), F10 (≠ A16), G11 (= G17), T12 (= T18), V13 (= V19), D39 (≠ V49), R40 (= R50), K57 (= K54), V91 (= V83), S92 (≠ M84), S93 (≠ G85), S114 (≠ A107), K116 (= K109), S141 (≠ A134), G295 (≠ A305), T300 (= T310)
O58802 Homoserine dehydrogenase; HDH; HSD; HseDH; EC 1.1.1.3 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see paper)
28% identity, 72% coverage: 9:319/430 of query aligns to 3:309/319 of O58802
- F10 (≠ A16) binding NADPH
- T12 (= T18) binding NADPH
- V13 (= V19) binding NADPH
- R40 (= R50) binding NADPH; mutation to A: Increases activity with NADP.
- K57 (= K54) binding NADPH; mutation to A: Increases activity with NADP.
- S92 (≠ M84) binding NADPH
- S93 (≠ G85) binding NADPH
- S114 (≠ A107) binding NADPH
- K116 (= K109) binding NADPH
- E140 (= E133) binding Na(+)
- V143 (= V136) binding Na(+)
- A145 (≠ G138) binding Na(+)
- T147 (≠ V140) binding Na(+)
- G296 (= G306) binding NADPH
4xb2A Hyperthermophilic archaeal homoserine dehydrogenase mutant in complex with NADPH (see paper)
28% identity, 72% coverage: 9:319/430 of query aligns to 3:309/319 of 4xb2A
- active site: D211 (= D205), K215 (= K209)
- binding l-homoserine: A171 (≠ G164), S172 (≠ T165), D206 (= D200), K215 (= K209)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: F8 (≠ L14), F10 (≠ A16), G11 (= G17), T12 (= T18), V13 (= V19), R40 (= R50), V91 (= V83), S92 (≠ M84), S93 (≠ G85), S114 (≠ A107), N115 (= N108), K116 (= K109), S141 (≠ A134), G295 (≠ A305), T300 (= T310)
3ingA Crystal structure of homoserine dehydrogenase (np_394635.1) from thermoplasma acidophilum at 1.95 a resolution
28% identity, 54% coverage: 7:239/430 of query aligns to 1:245/319 of 3ingA
- active site: D209 (= D205), K213 (= K209)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G9 (= G15), T10 (≠ A16), G11 (= G17), N12 (≠ T18), V13 (= V19), D38 (≠ E32), S39 (≠ D33), K57 (= K54), C85 (≠ D74), T86 (≠ D75), P87 (≠ N76), A112 (= A107), N113 (= N108), K114 (= K109), A139 (= A134), E198 (= E194), S199 (≠ A195)
Sites not aligning to the query:
3jsaA Homoserine dehydrogenase from thermoplasma volcanium complexed with NAD
34% identity, 33% coverage: 92:232/430 of query aligns to 102:239/321 of 3jsaA
Sites not aligning to the query:
- binding nicotinamide-adenine-dinucleotide: 10, 11, 12, 13, 14, 42, 43, 90, 91, 92, 308
O81852 Bifunctional aspartokinase/homoserine dehydrogenase 2, chloroplastic; AK-HD 2; AK-HSDH 2; Beta-aspartyl phosphate homoserine 2; EC 2.7.2.4; EC 1.1.1.3 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
25% identity, 54% coverage: 92:325/430 of query aligns to 656:912/916 of O81852
Sites not aligning to the query:
- 441 I→A: Loss of threonine sensitivity for the aspartokinase activity and decreased inhibition of homoserine dehydrogenase activity by threonine.
- 443 Q→A: Loss of threonine sensitivity for the aspartokinase activity and decreased inhibition of homoserine dehydrogenase activity by threonine.
- 522 I→A: No effect on the inhibition of aspartokinase activity by threonine, but decreased inhibition of homoserine dehydrogenase activity by threonine.
- 524 Q→A: No effect on the inhibition of aspartokinase activity by threonine, but decreased inhibition of homoserine dehydrogenase activity by threonine.
O94671 Homoserine dehydrogenase; HDH; HSD; EC 1.1.1.3 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
30% identity, 27% coverage: 103:217/430 of query aligns to 117:239/376 of O94671
- S201 (= S179) modified: Phosphoserine
1tveA Homoserine dehydrogenase in complex with 4-(4-hydroxy-3- isopropylphenylthio)-2-isopropylphenol (see paper)
27% identity, 49% coverage: 7:217/430 of query aligns to 3:230/358 of 1tveA
1q7gA Homoserine dehydrogenase in complex with suicide inhibitor complex NAD-5-hydroxy-4-oxonorvaline (see paper)
27% identity, 49% coverage: 7:217/430 of query aligns to 3:230/358 of 1q7gA
- active site: D218 (= D205), K222 (= K209)
- binding nicotinamide-adenine-dinucleotide-5-hydroxy-4-oxonorvaline: G13 (= G17), V14 (≠ T18), V15 (= V19), E39 (≠ S53), N91 (≠ V83), T92 (≠ M84), S93 (≠ G85), I97 (≠ P89), P114 (≠ A107), K116 (= K109), A143 (= A134), S173 (≠ N163), K222 (= K209)
Sites not aligning to the query:
1ebuD Homoserine dehydrogenase complex with NAD analogue and l-homoserine (see paper)
27% identity, 49% coverage: 7:217/430 of query aligns to 3:230/358 of 1ebuD
- active site: D218 (= D205), K222 (= K209)
- binding 3-aminomethyl-pyridinium-adenine-dinucleotide: G11 (= G15), A12 (= A16), G13 (= G17), V14 (≠ T18), V15 (= V19), E39 (≠ S53), A40 (≠ K54), N91 (≠ V83), S93 (≠ G85), K116 (= K109)
Sites not aligning to the query:
1ebfA Homoserine dehydrogenase from s. Cerevisiae complex with NAD+ (see paper)
27% identity, 49% coverage: 7:217/430 of query aligns to 3:230/358 of 1ebfA
- active site: D218 (= D205), K222 (= K209)
- binding nicotinamide-adenine-dinucleotide: I10 (≠ L14), A12 (= A16), G13 (= G17), V14 (≠ T18), V15 (= V19), E39 (≠ S53), A40 (≠ K54), T92 (≠ M84), S93 (≠ G85), P114 (≠ A107)
Query Sequence
>WP_005350757.1 NCBI__GCF_000173975.1:WP_005350757.1
METAQNKIVKAALLGAGTVGSGVYQLVQERQEDFSHICGTQIQITKILVRDASKEREGIP
SPLLTDNWNEIIEDDNISIIIEVMGGIEPAKSYLLEAMKAGKQIVTANKDLIAEHGHELL
DSAEKYGCDFKFEAAVAGCVPIIQVLKQSMSSENITEVMGIVNGTTNYILTRMTQSGMSY
DEALKEATDLGYAEADPTADVDGLDAGRKIAIMASIAFHSRVTFSDVYIEGIRNITAKDI
FYAKEFNSVIKLVGIARKDEDGIEVKVLPILIPQDHPLATVNDSFNAVFVHGTASDDTMY
YGRGAGKRPTASAVTGDLCTVARHIVEKHSNLHVCSCYKELPVKNIQDTYSRFFLRLQVA
DRPGVLANITSVFGTSQVSIAQIIQKSRQNGNAELVIITDEVKELYFTDALNILKRLSIV
NSISSLIRVY
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory