SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_005351659.1 NCBI__GCF_000173975.1:WP_005351659.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P9WP55 O-acetylserine sulfhydrylase; OAS sulfhydrylase; OASS; Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine-specific cysteine synthase; Sulfide-dependent cysteine synthase; EC 2.5.1.47 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
60% identity, 97% coverage: 11:310/310 of query aligns to 10:307/310 of P9WP55

query
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P9WP55

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K44 (= K47) modified: N6-(pyridoxal phosphate)lysine
N74 (= N77) binding pyridoxal 5'-phosphate
GTGGT 178:182 (= GTGGT 181:185) binding pyridoxal 5'-phosphate
S266 (= S269) binding pyridoxal 5'-phosphate

2q3dA 2.2 a resolution crystal structure of o-acetylserine sulfhydrylase (oass) from mycobacterium tuberculosis in complex with the reaction intermediate alpha-aminoacrylate (see paper)
60% identity, 96% coverage: 11:308/310 of query aligns to 10:305/306 of 2q3dA

query
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2q3dA

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active site: K44 (= K47), S266 (= S269), P293 (= P296)
binding 2-[(3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl)-amino]-propionic acid: K44 (= K47), T71 (= T74), S72 (= S75), N74 (= N77), T75 (= T78), Q144 (= Q147), V177 (= V180), G178 (= G181), T179 (= T182), G180 (= G183), T182 (= T185), G222 (= G225), I223 (= I226), S266 (= S269), P293 (= P296), D294 (= D297)

3zeiA Structure of the mycobacterium tuberculosis o-acetylserine sulfhydrylase (oass) cysk1 in complex with a small molecule inhibitor (see paper)
60% identity, 94% coverage: 11:302/310 of query aligns to 10:299/300 of 3zeiA

query
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3zeiA

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active site: K44 (= K47), S266 (= S269), P293 (= P296)
binding 3-[(Z)-[(5Z)-5-[[2-(2-hydroxy-2-oxoethyloxy)phenyl]methylidene]-3-methyl-4-oxidanylidene-1,3-thiazolidin-2-ylidene]amino]benzoic acid: T71 (= T74), S72 (= S75), I126 (= I129), Q144 (= Q147), F145 (= F148), K215 (≠ A218), G222 (= G225), A225 (= A228), F227 (= F230)
binding pyridoxal-5'-phosphate: K44 (= K47), N74 (= N77), V177 (= V180), G178 (= G181), T179 (= T182), G180 (= G183), T182 (= T185), G222 (= G225), S266 (= S269), P293 (= P296), D294 (= D297)

2q3cA 2.1 a resolution crystal structure of o-acetylserine sulfhydrylase (oass) holoenzyme from mycobacterium tuberculosis in complex with the inhibitory peptide dfsi (see paper)
60% identity, 94% coverage: 11:302/310 of query aligns to 10:299/300 of 2q3cA

query
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2q3cA

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active site: K44 (= K47), S266 (= S269), P293 (= P296)
binding : T71 (= T74), S72 (= S75), G73 (= G76), T75 (= T78), M122 (= M125), Q144 (= Q147), K215 (≠ A218), G222 (= G225), A225 (= A228)

5xoqA Crystal structure of o-acetylserine sulfhydrylase with bound transcription factor peptide inhibitor from planctomyces limnophilus
59% identity, 98% coverage: 4:308/310 of query aligns to 4:306/310 of 5xoqA

query
sites
5xoqA

I

I

Y

F

K

K

G

D

T

N

L

S

G

E

L

S

I

I

G

G

N

R

T

T

P

P

L

L

V

V

E

Q

V

I

A

N

N

R

I

L

E

T

K

A

E

-

L

-

G

G

L

L

K

S

A

S

R

R

V

V

L

L

V

A

K

K

L

I

E

E

Y

G

F

R

N

N

P

P

A

A

G

Y

S

S

V

V

K

K

D

C

R

R

I

I

A

G

K

A

A

A

M

M

I

I

E

W

D

D

A

A

E

E

K

Q

K

S

G

G

I

K

L

L

K

K

E

P

G

G

S

M

V

H

I

V

E

E

P

P

T
|
T

S
|
S

G
|
G

N

N

T
|
T

G

G

I

I

G

A

L

L

A

A

S

F

I

V

A

C

A

A

K

R

G

G

Y

Y

R

K

I

L

I

T

L

L

T

T

M

M

P

P

E

E

T

T

M

M

S

S

V

I

E

E

R

R

N

M

I

M

L

L

K

K

A

S

Y

F

G

G

A

A

E

D

I

L

V

V

L

L

T

T

S

P

G

G

A

A

K

D

G

G

M
|
M

K

K

G

G

A

A

I

I

A

S

K

K

A

A

N

E

E

E

L

L

S

A

E

A

E

Q

I

-

E

P

G

G

S

W

F

F

I

I

P

P

G

Q

Q
|
Q

F

F

V

K

N

N

P

P

A

A

N

N

P

P

A

A

I

I

H

H

K

V

A

K

T

T

G

G

P

P

E

E

I

I

W

W

N

N

D

D

T

T

D

E

G

G

E

Q

V

V

D

D

I

V

F

F

I

V

A

A

G

G

V

V

G

G

T

T

G

G

T

T

L

I

T

T

G

G

T

V

G

A

Q

R

Y

F

L

L

R

K

-

H

E

E

Q

K

K

K

P

H

E

P

V

V

K

H

I

V

V

V

A

A

L

V

E

E

P

P

D

A

T

A

S

S

P

P

V

V

L

L

S

A

E

G

G

G

K

P

A

A

G

G

P
x
R

H
|
H

K

K

I

I

Q
|
Q

G
|
G

I

I

G

G

A
|
A

G

G

F

F

V

V

P

P

D

D

V

T

L

F

D

D

T

R

K

S

I

V

Y

V

D

D

E

E

I

I

F

L

R

S

A

V

T

T

N

D

E

D

D

E

A

A

F

I

A

E

T

T

A

A

K

R

L

K

L

L

A

A

K

M

K

E

E

E

G

G

I

I

L

S

V

C

G

G

I

I

S

S

S

C

G

G

A

A

S

A

L

M

H

A

A

G

A

A

I

L

E

K

Y

V

A

A

K

A

K

R

P

P

E

E

N

F

E

A

G

G

K

K

T

T

I

I

V

V

A

T

L

V

L

L

P

P

D

D

T

A

G

G

D

E

R

R

Y

Y

Y

L

S

S

T

T

A

A

L

L

F

F

binding : T72 (= T74), S73 (= S75), G74 (= G76), T76 (= T78), M123 (= M125), Q144 (= Q147), R218 (≠ P220), H219 (= H221), Q222 (= Q224), G223 (= G225), A226 (= A228)

4lmaA Crystal structure analysis of o-acetylserine sulfhydrylase cysk1 from microcystis aeruginosa 7806 (see paper)
57% identity, 96% coverage: 11:308/310 of query aligns to 10:308/318 of 4lmaA

query
sites
4lmaA

L

L

I

V

G

G

N

R

T

T

P

P

L

L

V

V

E

Q

V

L

A

N

N

R

I

I

E

P

K

Q

E

A

L

E

G

G

L

C

K

L

A

G

R

R

V

I

L

V

V

M

K

K

L

L

E

E

Y

G

F

M

N

N

P

P

A

A

G

A

S

S

V

V

K
|
K

D

D

R

R

I

I

A

G

K

T

A

H

M

M

I

I

E

N

D

S

A

A

E

E

K

K

K

A

G

G

I

L

L

I

K

N

-

P

E

E

G

T

S

T

V

V

I

L

I

V

E

E

P

P

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

G

A

L

L

A

A

S

M

I

T

A

A

K

K

G

G

Y

Y

R

R

I

L

I

I

L

L

T

T

M

M

P

P

E

E

T

T

M

M

S

S

V

L

E

E

R

R

N

A

I

M

L

L

K

K

A

A

Y

Y

G

G

A

A

E

T

I

L

V

E

L

L

T

T

S

P

G

G

A

S

K

Q

G

G

M

M

K

K

G

G

A

A

I

I

A

L

K

R

A

A

N

Q

E

Q

L

I

S

V

E

D

E

S

I

I

E

P

G

G

S

A

F

Y

I

M

P

L

G

Q

Q

Q

F

F

V

R

N

N

P

P

A

S

N

N

P

P

A

E

I

I

H

H

K

R

A

L

T

T

G

A

P

E

E

E

I

I

W

W

N

Q

D

D

T

T

D

E

G

G

E

Q

V

V

D

D

I

F

F

I

I

V

A

A

G

G

V

V

G
|
G

T
|
T

G
|
G

G

G

T
|
T

L

I

T

T

G

G

T

V

G

A

Q

E

Y

V

L

I

R

K

E

S

Q

R

K

K

P

P

E

S

V

F

K

Q

I

V

V

V

A

A

L

V

E

E

P

P

D

F

T

N

S

S

P

P

V

V

L

I

S

S

E

G

G

G

K

N

A

P

G

G

P

P

H

H

K

K

I

I

Q

Q

G
|
G

I

I

G

G

A

A

G

G

F

F

V

I

P

P

D

E

V

V

L

L

D

R

T

T

K

D

I

L

Y

I

D

D

E

E

I

V

F

I

R

T

A

V

T

S

N

D

E

E

D

E

A

A

F

F

A

Q

T

F

A

G

K

R

L

R

L

L

A

A

K

K

K

E

E

E

G

G

I

L

L

L

V

S

G

G

I

I

S
|
S

S

S

G

G

A

A

S

N

L

L

H

C

A

A

I

I

E

Q

Y

L

A

A

K

Q

K

R

P

P

E

E

N

N

E

E

G

G

K

K

T

L

I

I

V

V

A

V

L

I

L

Q

P
|
P

D

S

T

F

G

G

D

E

R

R

Y

Y

Y

L

S

S

T

T

A

L

L

M

F

F

active site: K46 (= K47), S269 (= S269)
binding pyridoxal-5'-phosphate: K46 (= K47), N77 (= N77), G181 (= G181), T182 (= T182), G183 (= G183), T185 (= T185), G225 (= G225), S269 (= S269), P296 (= P296)

7n2tA O-acetylserine sulfhydrylase from citrullus vulgaris in the internal aldimine state, with citrate bound (see paper)
58% identity, 99% coverage: 2:308/310 of query aligns to 1:306/309 of 7n2tA

query
sites
7n2tA

A

S

N

T

I

I

Y

A

K

K

G

D

T

V

L

T

G

E

L

L

I

I

G

G

N

N

T

T

P

P

L

L

V

V

E

Y

V

L

A

N

N

R

I

V

E

V

K

D

E

-

L

-

G

G

L

C

K

V

A

A

R

R

V

V

L

A

V

A

K

K

L

L

E

E

Y

M

F

M

N

E

P

P

A

C

G

S

S

S

V

V

K

K

D

D

R

R

I

I

A

G

K

Y

A

S

M

M

I

I

E

S

D

D

A

A

E

E

K

N

K

K

G

G

I

L

L

I

K

T

E

P

G

G

-

E

S

S

V

V

I

L

I

I

E

E

P

P

T

T

S

S

G

G

N

N

T

T

G

G

I

I

G

G

L

L

A

A

S

F

I

I

A

A

K

K

G

G

Y

Y

R

R

I

L

I

I

L

I

T

C

M

M

P

P

E

A

T

S

M

M

S

S

V

L

E

E

R

R

N

T

I

I

L

L

K

R

A

A

Y

F

G

G

A

A

E

E

I

L

V

V

L

L

T

T

S

D

G

P

A

A

K

R

G

G

M

M

K

K

G

G

A

A

I

V

A

Q

K

K

A

A

N

E

E

E

L

I

S

K

E

A

E

K

I

T

E

P

G

N

S

S

F

Y

I

I

P

L

G

Q

Q

Q

F

F

V

E

N

N

P

P

A

A

N

N

P

P

A

K

I

I

H

H

K

Y

A

E

T

T

G

G

P

P

E

E

I

I

W

W

N

R

D

G

T

S

D

G

G

G

E

K

V

I

D

D

I

A

F

L

I

V

A

S

G

G

V

I

G

G

T

T

G

G

T

T

L

V

T

T

G

G

T

A

G

G

Q

K

Y

Y

L

L

R

K

E

E

Q

Q

K

N

P

P

E

N

V

I

K

K

I

L

V

Y

A

G

L

V

E

E

P

P

D

V

T

E

S

S

P

A

V

I

L

L

S

S

E

G

G

G

K

K

A

P

G

G

P

P

H

H

K

K

I

I

Q

Q

G

G

I

I

G

G

A

A

G

G

F

F

V

I

P

P

D

G

V

V

L

L

D

D

T

V

K

N

I

L

Y

L

D

D

E

E

I

V

F

I

R

Q

A

V

T

S

N

S

E

E

D

E

A

S

F

I

A

E

T

T

A

A

K

K

L

L

A

A

K

L

K

K

E

E

G

G

I

L

L

L

V

V

G

G

I

I

S

S

G

G

A

A

S

A

L

A

H

A

A

A

I

I

E
x
R

Y

I

A

A

K

K

K
x
R

P

P

E

E

N

N

E

A

G

G

K

K

T

L

I

I

V

V

A

A

L

V

L

F

P

P

D

S

T

F

G

G

D

E

R

R

Y

Y

Y

L

S

S

T

T

A

V

L

L

F

F

binding phosphate ion: R277 (≠ E279), R281 (≠ K283)

Q93244 Cysteine synthase 1; O-acetylserine (thiol)-lyase 1; OAS-TL; EC 2.5.1.47 from Caenorhabditis elegans (see 2 papers)
58% identity, 97% coverage: 11:310/310 of query aligns to 14:312/341 of Q93244

query
sites
Q93244

L

L

I

I

G

G

N

N

T

T

P

P

L

M

V

V

E

Y

V

I

A

N

N

K

I

L

E

T

K

K

E

-

L

-

G

G

L

L

K

P

A

G

R

T

V

V

L

A

V

V

K

K

L

I

E

E

Y

Y

F

M

N

N

P

P

A

A

G

G

S

S

V

V

K

K

D

D

R

R

I

I

A

G

K

A

A

A

M

M

I

L

E

A

D

A

A

A

E

E

K

K

K

D

G

G

I

T

L

V

K

I

E

P

G

G

-

V

S

T

V

T

I

L

I

I

E

E

P
|
P

T

T

S

S

G

G

N

N

T

T

G

G

I

I

G

A

L

L

A

A

S

F

I

V

A
|
A

A

A

K

K

G

G

Y

Y

R

R

I

C

I

I

L

V

T

T

M

M

P

P

E

A

T

S

M

M

S

S

V

G

E

E

R

R

N

T

I

L

L

L

K

K

A

A

Y

Y

G

G

A

S

E

E

I

V

V

V

L

L

T

T

S

D

G

P

A

A

K

K

G

G

M

M

K

K

G

G

A

A

I

I

A

D

K

M

A

A

N

N

E

Q

L

L

S

K

E

E

E

N

I

I

E

P

G

G

S
|
S

F

I

I

I

P

L

G

A

Q

Q

F

F

V

D

N

N

P

P

A

N

N

N

P

P

A

L

I

V

H

H

K

Y

A

Q

T

T

G

G

P

P

E

E

I

I

W

W

N

R

D

Q

T

T

D

K

G

G

E

T

V

V

D

D

I

A

F

V

I

V

A

F

G
|
G

V

V

G
|
G

T

T

G

G

T

T

L

I

T

T

G

G

T

V

G

G

Q

R

Y

Y

L

L

R

Q

E

E

Q

Q

K

N

P

P

E

G

V

V

K

R

I

V

V

F

A

A

L

V

E

E

P

P

D

E

T

E

S

S

P

A

V

I

L

L

S

S

E

G

G

R

K

P

A

A

G

G

P

P

H

H

K

K

I

I

Q

Q

G

G

I

I

G
|
G

A

A

G

G

F

F

V

A

P

P

D

A

V

V

L

L

D

D

T

T

K

K

I

I

Y

Y

D

E

E

D

I

V

F

I

R

R

A

I

T

H

N

S

E

D

D

E

A

A

F

I

A

V

T

M

A

A

K

Q

L
x
R

L

L

A

S

K

Y

K

E

E

E

G

G

I

L

L

L

V

G

G

G

I

I

S

S

S
|
S

G

G

A

A

S

N

L

V

H

A

A

A

I

L

E

Q

Y

L

A

A

K

A

K

R

P

P

E

E

N

M

E

A

G

G

K

K

T

L

I

I

V

V

A
x
T

L

C

L

L

P

P

D

S

T

C

G

G

D

E

R

R

Y

Y

Y

M

S

T

T

S

A

P

L

L

F

Y

T

T

E

D

P75 (= P73) mutation to L: In n5537; severe loss of protein stability.
A88 (= A86) mutation to V: In n5522; severe loss of protein stability.
S144 (= S142) mutation to F: In mr26; susceptible to high levels of hydrogen sulfide.
G181 (= G179) mutation to E: In n5521 and mr23; severe loss of protein stability. Susceptible to high levels of hydrogen sulfide.
G183 (= G181) mutation to R: In n5515; severe loss of protein stability.
G229 (= G227) mutation to E: In mr33; susceptible to high levels of hydrogen sulfide.
R259 (≠ L257) mutation to K: In n5519; no loss of protein stability. No effect on enzyme activity.
S272 (= S270) mutation to F: In mr29; susceptible to high levels of hydrogen sulfide.
T295 (≠ A293) mutation to I: In mr39; susceptible to high levels of hydrogen sulfide.

4lmbA Crystal structure analysis of o-acetylserine sulfhydrylase cysk2 complexed with cystine from microcystis aeruginosa 7806 (see paper)
55% identity, 99% coverage: 4:310/310 of query aligns to 3:310/310 of 4lmbA

query
sites
4lmbA

I

I

Y

A

K

R

G

D

T

I

L

T

G

Q

L

L

I

V

G

G

N

R

T

T

P

P

L

L

V

V

E

Q

V

L

A

N

N

R

I

I

E

P

K

V

E

A

L

E

G

G

L

V

K

K

A

A

R

R

V

I

L

V

V

V

K

K

L

L

E

E

Y

S

F

M

N

N

P

P

A

A

G

A

S

S

V

V

K
|
K

D

D

R

R

I

I

A

G

K

V

A

S

M

M

I

V

E

E

D

D

A

A

E

E

K

A

G

G

I

L

L

I

K

H

-

P

E

D

G

K

S

T

V

I

I

L

I

V

E

E

P

P

T
|
T

S
|
S

G

G

N
|
N

T
|
T

G

G

I

I

G

A

L

L

A

A

S

M

I

V

A

A

K

K

G

G

Y

Y

R

R

I

L

I

V

L

L

T

T

M

M

P

P

E

E

T

T

M
|
M

S

S

V

L

E

E

R

R

N

A

I

M

L

L

K

K

A

A

Y

Y

G

G

A

A

E

Q

I

L

V

E

L

L

T

T

S

P

G

G

A

S

K

Q

G

G

M
|
M

K

E

G

G

A

A

I

I

A

T

K

R

A

A

N

E

E

E

L

I

S

V

E

E

E

N

I

T

E

P

G

N

S

A

F

Y

I

S

P

L

G

Q

Q
|
Q

F
|
F

V

R

N

N

P

P

A

A

N

N

P

P

A

K

I

I

H

H

K

R

A

E

T

T

G

A

P

E

E

E

I

I

W

W

N

A

D

D

T

T

D

D

G

G

E

L

V

V

D

D

I

I

F

V

I

I

A

G

G

G

V
|
V

G
|
G

T
|
T

G
|
G

G

G

T
|
T

L

I

T

T

G

G

T

I

G

A

Q

E

Y

T

L

I

R

K

E

P

Q

R

K

R

P

P

E

Q

V

F

K

Q

I

A

V

I

A

A

L

V

E

E

P

P

D

S

T

N

S

S

P

P

V

V

L

L

S

S

E

G

G

G

K

Q

A

P

G

G

P

P

H

H

K

K

I

I

Q
|
Q

G
|
G

I
|
I

G

G

A
|
A

G

G

F

F

V

I

P

P

D

A

V

I

L

F

D

R

T

P

K

E

I

L

Y

I

D

D

E

E

I

V

F

I

R

I

A

V

T

D

N

D

E

T

D

E

A

A

F

F

A

A

T

Y

A

A

K

R

L

R

L

L

A

A

K

R

K

Q

E

E

G

G

I

L

L

L

V

S

G

G

I

I

S
|
S

S

A

G

G

A

A

S

A

L

L

H

W

A

A

I

I

E

Q

Y

V

A

G

K

K

K

R

P

P

E

E

N

N

E

E

G

D

K

K

T

L

I

I

V

V

A

M

L

I

L

Q

P
|
P

D

S

T

F

G

G

D

E

R

R

Y

Y

Y

L

S

S

T

T

A

A

L

L

F

F

T

K

E

D

active site: K46 (= K47), S269 (= S269)
binding cysteine: K46 (= K47), T74 (= T74), S75 (= S75), N77 (= N77), T78 (= T78), M101 (= M101), M125 (= M125), M125 (= M125), Q147 (= Q147), F148 (= F148), Q224 (= Q224), G225 (= G225), G225 (= G225), I226 (= I226), A228 (= A228)
binding pyridoxal-5'-phosphate: K46 (= K47), N77 (= N77), V180 (= V180), G181 (= G181), T182 (= T182), G183 (= G183), T185 (= T185), G225 (= G225), S269 (= S269), P296 (= P296)

4aecA Crystal structure of the arabidopsis thaliana o-acetyl-serine-(thiol)- lyasE C (see paper)
57% identity, 99% coverage: 3:308/310 of query aligns to 12:316/323 of 4aecA

query
sites
4aecA

N

N

I

I

Y

A

K

D

G

N

T

V

L

S

G

Q

L

L

I

I

G

G

N

K

T

T

P

P

L

M

V

V

E

Y

V

L

A

N

N

S

I

I

E

A

K

K

E

-

L

-

G

G

L

C

K

V

A

A

R

N

V

I

L

A

V

A

K

K

L

L

E

E

Y

I

F

M

N

E

P

P

A

C

G

C

S

S

V

V

K
|
K

D

D

R

R

I

I

A

G

K

Y

A

S

M

M

I

V

E

T

D

D

A

A

E

E

K

Q

K

K

G

G

I

F

L

I

K

S

E

P

G

G

-

K

S

S

V

V

I

L

I

V

E

E

P

P

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

G

G

L

L

A

A

S

F

I

I

A

A

A

S

K

R

G

G

Y

Y

R

R

I

L

I

I

L

L

T

T

M

M

P

P

E

A

T

S

M

M

S

S

V

M

E

E

R

R

N

V

I

L

L

L

K

K

A

A

Y

F

G

G

A

A

E

E

I

L

V

V

L

L

T

T

S

D

G

P

A

A

K

K

G

G

M

M

K

T

G

G

A

A

I

V

A

Q

K

K

A

A

N

E

E

E

L

I

S

L

E

K

E

N

I

T

E

P

G

D

S

A

F

Y

I

M

P

L

G

Q

Q

Q

F

F

V

D

N

N

P

P

A

A

N

N

P

P

A

K

I

I

H

H

K

Y

A

E

T

T

G

G

P

P

E

E

I

I

W

W

N

D

D

D

T

T

D

K

G

G

E

K

V

V

D

D

I

I

F

F

I

V

A

A

G

G

V
x
I

G
|
G

T
|
T

G
|
G

T
|
T

L

I

T

T

G

G

T

V

G

G

Q

R

Y

F

L

I

R

K

E

E

Q

K

K

N

P

P

E

K

V

T

K

Q

I

V

V

I

A

G

L

V

E

E

P

P

D

T

T

E

S

S

P

D

V

I

L

L

S

S

E

G

G

G

K

K

A

P

G

G

P

P

H

H

K

K

I

I

Q

Q

G
|
G

I

I

G

G

A

A

G

G

F

F

V

I

P

P

D

K

V

N

L

L

D

D

T

Q

K

K

I

I

Y

M

D

D

E

E

I

V

F

I

R

A

A

I

T

S

N

S

E

E

D

E

A

A

F

I

A

E

T

T

A

A

K

K

L

Q

L

L

A

A

K

L

K

K

E

E

G

G

I

L

L

M

V

V

G

G

I

I

S
|
S

S

S

G

G

A

A

S

A

L

A

H

A

A

A

I

I

E

K

Y

V

A

A

K

K

K

R

P

P

E

E

N

N

E

A

G

G

K

K

T

L

I

I

V

A

A

V

L

V

L

F

P
|
P

D

S

T

F

G

G

D

E

R

R

Y

Y

Y

L

S

S

T

T

A

P

L

L

F

F

active site: K54 (= K47), S277 (= S269)
binding pyridoxal-5'-phosphate: K54 (= K47), N85 (= N77), I188 (≠ V180), G189 (= G181), T190 (= T182), G191 (= G183), G192 (= G184), T193 (= T185), G233 (= G225), S277 (= S269), P304 (= P296)

P47999 Cysteine synthase, chloroplastic/chromoplastic; At.OAS.7-4; Beta-substituted Ala synthase 2;1; ARAth-Bsas2;1; CSase B; AtCS-B; CS-B; O-acetylserine (thiol)-lyase; O-acetylserine sulfhydrylase; OAS-TL B; cpACS1; EC 2.5.1.47 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
57% identity, 99% coverage: 3:308/310 of query aligns to 74:378/392 of P47999

query
sites
P47999

N

N

I

I

Y

A

K

D

G

N

T

A

L

A

G

Q

L

L

I

I

G

G

N

K

T

T

P

P

L

M

V

V

E

Y

V

L

A

N

N

N

I

V

E

V

K

K

E

-

L

-

G

G

L

C

K

V

A

A

R

S

V

V

L

A

V

A

K

K

L

L

E

E

Y

I

F

M

N

E

P

P

A

C

G

C

S

S

V

V

K

K

D

D

R

R

I

I

A

G

K

Y

A

S

M

M

I

I

E

T

D

D

A

A

E

E

K

E

K

K

G

G

I

L

L

I

K

T

E

P

G

G

-

K

S

S

V

V

I

L

I

V

E

E

P

S

T

T

S

S

G

G

N

N

T

T

G

G

I

I

G

G

L

L

A

A

S

F

I

I

A

A

A

S

K

K

G

G

Y

Y

R

K

I

L

I

I

L

L

T

T

M

M

P

P

E

A

T

S

M

M

S

S

V

L

E

E

R

R

N

V

I

L

L

L

K

R

A

A

Y

F

G

G

A

A

E

E

I

L

V

V

L

L

T

T

S

E

G

P

A

A

K

K

G

G

M

M

K

T

G

G

A

A

I

I

A

Q

K

K

A

A

N

E

E

E

L

I

S

L

E

K

I

T

E

P

G

N

S

S

F

Y

I

M

P

L

G

Q

Q

Q

F

F

V

D

N

N

P

P

A

A

N

N

P

P

A

K

I

I

H

H

K

Y

A

E

T

T

G

G

P

P

E

E

I

I

W

W

N

E

D

D

T

T

D

R

G

G

E

K

V

I

D

D

I

I

F

L

I

V

A

A

G

G

V

I

G

G

T

T

G

G

T

T

L

I

T

T

G

G

T

V

G

G

Q

R

Y

F

L

I

R

K

E

E

Q

R

K

K

P

P

E

E

V

L

K

K

I

V

V

I

A

G

L

V

E

E

P

P

D

T

T

E

S

S

P

A

V

I

L

L

S

S

E

G

G

G

K

K

A

P

G

G

P

P

H

H

K

K

I

I

Q

Q

G

G

I

I

G

G

A

A

G

G

F

F

V

V

P

P

D

K

V

N

L

L

D

D

T

L

K

A

I

I

Y

V

D

D

E

E

I

Y

F

I

R

A

A

I

T

S

N

S

E

E

D

E

A

A

F

I

A

E

T

T

A

S

K

K

L

Q

L

L

A

A

K

L

K

Q

E

E

G

G

I

L

L

L

V

V

G

G

I

I

S

S

G

G

A

A

S

A

L

A

H

A

A

A

I

I

E

Q

Y

V

A

A

K

K

K

R

P

P

E

E

N

N

E

A

G

G

K

K

T

L

I

I

V

A

A

V

L

V

L

F

P

P

D

S

T

F

G

G

D

E

R

R

Y

Y

Y

L

S

S

T

T

A

Q

L

L

F

F

Sites not aligning to the query:

61 modified: N-acetylalanine

P47998 Cysteine synthase 1; At.OAS.5-8; Beta-substituted Ala synthase 1;1; ARAth-Bsas1;1; CSase A; AtCS-A; Cys-3A; O-acetylserine (thiol)-lyase 1; OAS-TL A; O-acetylserine sulfhydrylase; Protein ONSET OF LEAF DEATH 3; EC 2.5.1.47 from Arabidopsis thaliana (Mouse-ear cress) (see 3 papers)
57% identity, 99% coverage: 2:308/310 of query aligns to 3:308/322 of P47998

query
sites
P47998

A

S

N

R

I

I

Y

A

K

K

G

D

T

V

L

T

G

E

L

L

I

I

G

G

N

N

T

T

P

P

L

L

V

V

E

Y

V

L

A

N

N

N

I

V

E

A

K

E

E

-

L

-

G

G

L

C

K

V

A

G

R

R

V

V

L

A

V

A

K

K

L

L

E

E

Y

M

F

M

N

E

P

P

A

C

G

S

S

S

V

V

K
|
K

D

D

R

R

I

I

A

G

K

F

A

S

M

M

I

I

E

S

D

D

A

A

E

E

K

K

G

G

I

L

L

I

K

K

E

P

G

G

-

E

S

S

V

V

I

L

I

I

E

E

P

P

T
|
T

S
|
S

G

G

N
|
N

T
|
T

G

G

I

V

G

G

L

L

A

A

S

F

I

T

A

A

K

K

G

G

Y

Y

R

K

I

L

I

I

L

I

T

T

M

M

P

P

E

A

T

S

M

M

S

S

V

T

E

E

R

R

N

I

I

I

L

L

K

L

A

A

Y

F

G

G

A

V

E

E

I

L

V

V

L

L

T

T

S

D

G

P

A

A

K

K

G

G

M

M

K

K

G

G

A

A

I

I

A

A

K

K

A

A

N

E

E

E

L

I

S

L

E

A

E

K

I

T

E

P

G

N

S

G

F

Y

I

M

P

L

G

Q

Q
|
Q

F

F

V

E

N

N

P

P

A

A

N

N

P

P

A

K

I

I

H
|
H

K

Y

A

E

T

T

G
|
G

P

P

E

E

I

I

W

W

N

K

D

G

T

T

D

G

G

G

E

K

V

I

D

D

I

G

F

F

I

V

A

S

G

G

V

I

G
|
G

T
|
T

G
|
G

T
|
T

L

I

T

T

G

G

T

A

G

G

Q

K

Y

Y

L

L

R

K

E

E

Q

Q

K

N

P

A

E

N

V

V

K

K

I

L

V

Y

A

G

L

V

E

E

P

P

D

V

T

E

S

S

P

A

V

I

L

L

S

S

E

G

G

G

K
|
K

A

P

G

G

P

P

H
|
H

K
|
K

I

I

Q

Q

G

G

I

I

G

G

A

A

G

G

F

F

V

I

P

P

D

S

V

V

L

L

D

N

T

V

K

D

I

L

Y

I

D

D

E

E

I

V

F

V

R

Q

A

V

T

S

N

S

E

D

D

E

A

S

F

I

A

D

T

M

A

A

K

R

L

Q

L

L

A

A

K

L

K

K

E

E

G

G

I

L

L

L

V

V

G

G

I

I

S
|
S

S

S

G

G

A

A

S

A

L

A

H

A

A

A

I

I

E

K

Y

L

A

A

K

Q

K

R

P

P

E

E

N

N

E

A

G

G

K

K

T

L

I

F

V

V

A

A

L

I

L

F

P

P

D

S

T

F

G

G

D

E

R

R

Y

Y

Y

L

S

S

T

T

A

V

L

L

F

F

K46 (= K47) modified: N6-(pyridoxal phosphate)lysine; mutation to A: No cysteine synthase activity.
T74 (= T74) mutation to A: Strong reduction of cysteine synthase activity.; mutation to S: Reduction of cysteine synthase activity.
S75 (= S75) mutation S->A,N,T: Strong reduction of cysteine synthase activity.
N77 (= N77) binding pyridoxal 5'-phosphate; mutation to A: Reduction of cysteine synthase activity.; mutation to D: Strong reduction of cysteine synthase activity.
T78 (= T78) mutation T->A,S: Reduction of cysteine synthase activity.
Q147 (= Q147) mutation Q->A,E: Strong reduction of cysteine synthase activity.
H157 (= H157) mutation H->Q,N: Slight reduction of cysteine synthase activity.
G162 (= G162) mutation to E: In old3-1; displays a early leaf death phenotype. Abolishes cysteine synthase activity.
GTGGT 181:185 (= GTGGT 181:185) binding pyridoxal 5'-phosphate
T182 (= T182) mutation T->A,S: Slight reduction of cysteine synthase activity.
T185 (= T185) mutation T->A,S: Strong reduction of cysteine synthase activity.
K217 (= K217) mutation to A: Impaired interaction with SAT1.
H221 (= H221) mutation to A: Impaired interaction with SAT1.
K222 (= K222) mutation to A: Impaired interaction with SAT1.
S269 (= S269) binding pyridoxal 5'-phosphate; mutation to A: Strong reduction of cysteine synthase activity.; mutation to T: Reduction of cysteine synthase activity.

2isqA Crystal structure of o-acetylserine sulfhydrylase from arabidopsis thaliana in complex with c-terminal peptide from arabidopsis serine acetyltransferase (see paper)
57% identity, 99% coverage: 2:308/310 of query aligns to 1:306/320 of 2isqA

query
sites
2isqA

A

S

N

R

I

I

Y

A

K

K

G

D

T

V

L

T

G

E

L

L

I

I

G

G

N

N

T

T

P

P

L

L

V

V

E

Y

V

L

A

N

N

N

I

V

E

A

K

E

E

-

L

-

G

G

L

C

K

V

A

G

R

R

V

V

L

A

V

A

K

K

L

L

E

E

Y

M

F

M

N

E

P

P

A

C

G

S

S

S

V

V

K
|
K

D

D

R

R

I

I

A

G

K

F

A

S

M

M

I

I

E

S

D

D

A

A

E

E

K

K

G

G

I

L

L

I

K

K

E

P

G

G

-

E

S

S

V

V

I

L

I

I

E

E

P

P

T
|
T

S
|
S

G
|
G

N
|
N

T
|
T

G

G

I

V

G

G

L

L

A

A

S

F

I

T

A

A

K

K

G

G

Y

Y

R

K

I

L

I

I

L

I

T

T

M

M

P

P

E

A

T

S

M

M

S

S

V

T

E

E

R

R

N

I

I

I

L

L

K

L

A

A

Y

F

G

G

A

V

E

E

I

L

V

V

L

L

T

T

S

D

G

P

A

A

K

K

G
|
G

M
|
M

K
|
K

G

G

A

A

I

I

A

A

K

K

A

A

N

E

E

E

L

I

S

L

E

A

E

K

I

T

E

P

G

N

S

G

F

Y

I

M

P

L

G

Q

Q

Q

F

F

V

E

N

N

P

P

A

A

N

N

P

P

A

K

I

I

H

H

K

Y

A

E

T

T

G

G

P

P

E

E

I

I

W

W

N

K

D

G

T

T

D

G

G

G

E

K

V

I

D

D

I

G

F

F

I

V

A

S

G
|
G

V

I

G
|
G

T
|
T

G
|
G

G

G

T
|
T

L

I

T

T

G

G

T

A

G

G

Q

K

Y

Y

L

L

R

K

E

E

Q

Q

K

N

P

A

E

N

V

V

K

K

I

L

V

Y

A

G

L

V

E

E

P

P

D

V

T

E

S

S

P

A

V

I

L

L

S

S

E

G

G

G

K

K

A

P

G
|
G

P
|
P

H
|
H

K

K

I

I

Q
|
Q

G
|
G

I

I

G

G

A

A

G

G

F

F

V

I

P

P

D

S

V

V

L

L

D

N

T

V

K

D

I

L

Y

I

D

D

E

E

I

V

F

V

R

Q

A

V

T

S

N

S

E

D

D

E

A

S

F

I

A

D

T

M

A

A

K

R

L

Q

L

L

A

A

K

L

K

K

E

E

G

G

I

L

L

L

V

V

G

G

I

I

S
|
S

S

S

G

G

A

A

S

A

L

A

H

A

A

A

I

I

E

K

Y

L

A

A

K

Q

K

R

P

P

E

E

N

N

E

A

G

G

K

K

T

L

I

F

V

V

A

A

L

I

L

F

P
|
P

D

S

T

F

G

G

D

E

R

R

Y

Y

Y

L

S

S

T

T

A

V

L

L

F

F

active site: K44 (= K47), S267 (= S269)
binding pyridoxal-5'-phosphate: K44 (= K47), N75 (= N77), G177 (= G179), G179 (= G181), T180 (= T182), G181 (= G183), T183 (= T185), G223 (= G225), S267 (= S269), P294 (= P296)
binding : T72 (= T74), S73 (= S75), G74 (= G76), T76 (= T78), G122 (= G124), M123 (= M125), K124 (= K126), G217 (= G219), P218 (= P220), H219 (= H221), Q222 (= Q224), G223 (= G225)

1z7yA Crystal structure of the arabidopsis thaliana o-acetylserine sulfhydrylase k46a mutant (see paper)
56% identity, 99% coverage: 2:308/310 of query aligns to 1:306/320 of 1z7yA

query
sites
1z7yA

A

S

N

R

I

I

Y

A

K

K

G

D

T

V

L

T

G

E

L

L

I

I

G

G

N

N

T

T

P

P

L

L

V

V

E

Y

V

L

A

N

N

N

I

V

E

A

K

E

E

-

L

-

G

G

L

C

K

V

A

G

R

R

V

V

L

A

V

A

K

K

L

L

E

E

Y

M

F

M

N

E

P

P

A

C

G

S

S

S

V

V

K
x
A

D

D

R

R

I

I

A

G

K

F

A

S

M

M

I

I

E

S

D

D

A

A

E

E

K

K

G

G

I

L

L

I

K

K

E

P

G

G

-

E

S

S

V

V

I

L

I

I

E

E

P

P

T

T

S

S

G
|
G

N
|
N

T
|
T

G

G

I

V

G

G

L

L

A

A

S

F

I

T

A

A

K

K

G

G

Y

Y

R

K

I

L

I

I

L

I

T

T

M

M

P

P

E

A

T

S

M

M

S

S

V

T

E

E

R

R

N

I

I

I

L

L

K

L

A

A

Y

F

G

G

A

V

E

E

I

L

V

V

L

L

T

T

S

D

G

P

A

A

K

K

G

G

M

M

K

K

G

G

A

A

I

I

A

A

K

K

A

A

N

E

E

E

L

I

S

L

E

A

E

K

I

T

E

P

G

N

S

G

F

Y

I

M

P

L

G

Q

Q
|
Q

F

F

V

E

N

N

P

P

A

A

N

N

P

P

A

K

I

I

H

H

K

Y

A

E

T

T

G

G

P

P

E

E

I

I

W

W

N

K

D

G

T

T

D

G

G

G

E

K

V

I

D

D

I

G

F

F

I

V

A

S

G

G

V
x
I

G
|
G

T
|
T

G
|
G

G

G

T
|
T

L

I

T

T

G

G

T

A

G

G

Q

K

Y

Y

L

L

R

K

E

E

Q

Q

K

N

P

A

E

N

V

V

K

K

I

L

V

Y

A

G

L

V

E

E

P

P

D

V

T

E

S

S

P

A

V

I

L

L

S

S

E

G

G

G

K

K

A

P

G

G

P

P

H

H

K

K

I

I

Q

Q

G
|
G

I

I

G

G

A

A

G

G

F

F

V

I

P

P

D

S

V

V

L

L

D

N

T

V

K

D

I

L

Y

I

D

D

E

E

I

V

F

V

R

Q

A

V

T

S

N

S

E

D

D

E

A

S

F

I

A

D

T

M

A

A

K

R

L

Q

L

L

A

A

K

L

K

K

E

E

G

G

I

L

L

L

V

V

G

G

I

I

S
|
S

S

S

G

G

A

A

S

A

L

A

H

A

A

A

I

I

E

K

Y

L

A

A

K

Q

K

R

P

P

E

E

N

N

E

A

G

G

K

K

T

L

I

F

V

V

A

A

L

I

L

F

P
|
P

D
x
S

T

F

G

G

D

E

R

R

Y

Y

Y

L

S

S

T

T

A

V

L

L

F

F

active site: A44 (≠ K47), S267 (= S269)
binding n-[(3-hydroxy-2-methyl-5-{[(trihydroxyphosphoranyl)oxy]methyl}pyridin-4-yl)methylene]methionine: G74 (= G76), N75 (= N77), T76 (= T78), Q145 (= Q147), I178 (≠ V180), G179 (= G181), T180 (= T182), G181 (= G183), T183 (= T185), G223 (= G225), S267 (= S269), P294 (= P296), S295 (≠ D297)

P0ABK5 Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine sulfhydrylase A; S-carboxymethylcysteine synthase; Sulfate starvation-induced protein 5; SSI5; EC 2.5.1.47; EC 4.5.1.5 from Escherichia coli (strain K12) (see 5 papers)
54% identity, 100% coverage: 1:310/310 of query aligns to 1:314/323 of P0ABK5

query
sites
P0ABK5

M
|
M

A

S

N

K

I

I

Y

F

K

E

G

D

T

N

L

S

G

L

L

T

I

I

G

G

N

H

T

T

P

P

L

L

V

V

E

R

V

L

A

N

N

R

I

I

E

G

K

N

E

-

L

-

G

-

L

-

K

-

A

G

R

R

V

I

L

L

V

A

K

K

L

V

E

E

Y

S

F

R

N

N

P

P

A

S

G

F

S

S

V

V

K
|
K

D

C

R

R

I

I

A

G

K

A

A

N

M

M

I

I

E

W

D

D

A

A

E

E

K

K

K

R

G

G

I

V

L

L

K

K

E

P

G

G

S

V

V

E

I

L

I

V

E

E

P

P

T

T

S

S

G

G

N

N

T

T

G

G

I

I

G

A

L

L

A

A

S

Y

I

V

A

A

K

R

G

G

Y

Y

R

K

I

L

I

T

L

L

T

T

M

M

P

P

E

E

T

T

M

M

S

S

V

I

E

E

R

R

N

K

I

L

L

L

K

K

A

A

Y

L

G

G

A

A

E

N

I

L

V

V

L

L

T

T

S

E

G

G

A

A

K

K

G

G

M

M

K

K

G

G

A

A

I

I

A

Q

K

K

A

A

N

E

E

E

L

I

-

V

S

A

E

S

E

N

I

P

E

E

G

K

S

Y

F

L

I

L

P

L

G

Q

Q

Q

F

F

V

S

N

N

P

P

A

A

N

N

P

P

A

E

I

I

H

H

K

E

A

K

T

T

G

G

P

P

E

E

I

I

W

W

N

E

D

D

T

T

D

D

G

G

E

Q

V

V

D

D

I

V

F

F

I

I

A

A

G

G

V

V

G

G

T

T

G

G

T

T

L

L

T

T

G

G

T

V

G

S

Q

R

Y

Y

L

I

R

K

E

G

Q

T

K

K

P

G

E

K

V

T

K

D

I

L

-

I

-

S

V

V

A

A

L

V

E

E

P

P

D

T

T

D

S

S

P

P

V

V

L

I

S

A

E

Q

G

A

-

L

-

A

-

G

-

E

-

I

K

K

A

P

G

G

P

P

H

H

K

K

I

I

Q

Q

G

G

I

I

G

G

A

A

G

G

F

F

V

I

P

P

D

A

V

N

L

L

D

D

T

L

K

K

I

L

Y

V

D

D

E

K

I

V

F

I

R

G

A

I

T

T

N

N

E

E

D

E

A

A

F

I

A

S

T

T

A

A

K

R

L

R

L

L

A

M

K

E

E

E

G

G

I

I

L

L

V

A

G

G

I

I

S

S

G

G

A

A

S

A

L

V

H

A

A

A

I

L

E

K

Y

L

A

Q

K

E

K

D

P

E

E

S

N

F

E

T

G

N

K

K

T

N

I

I

V

V

A

V

L

I

L

L

P

P

D

S

T

S

G

G

D

E

R

R

Y

Y

Y

L

S

S

T

T

A

A

L

L

F

F

T

A

E

D

M1 (= M1) modified: Initiator methionine, Removed
K42 (= K47) modified: N6-(pyridoxal phosphate)lysine; mutation to A: Still stimulates tRNase activity of CdiA-CT in vitro and in vivo.

6z4nAAA structure of oass complexed with upar inhibitor (see paper)
54% identity, 100% coverage: 1:310/310 of query aligns to 2:315/321 of 6z4nAAA

query
sites
6z4nAAA

M

M

A

S

N

K

I

I

Y

Y

K

E

G

D

T

N

L

S

G

L

L

T

I

I

G

G

N

H

T

T

P

P

L

L

V

V

E

R

V

L

A

N

N

R

I

I

E

G

K

N

E

-

L

-

G

-

L

-

K

-

A

G

R

R

V

I

L

L

V

A

K

K

L

V

E

E

Y

S

F

R

N

N

P

P

A

S

G

F

S

S

V

V

K
|
K

D

C

R

R

I

I

A

G

K

A

A

N

M

M

I

I

E

W

D

D

A

A

E

E

K

K

K

R

G

G

I

V

L

L

K

K

E

P

G

G

S

V

V

E

I

L

I

V

E

E

P

P

T
|
T

S

S

G
|
G

N
|
N

T
|
T

G

G

I

I

G

A

L

L

A

A

S

Y

I

V

A

A

K

R

G

G

Y

Y

R

K

I

L

I

T

L

L

T

T

M

M

P

P

E

E

T

T

M

M

S

S

V

I

E

E

R

R

N

K

I

L

L

L

K

K

A

A

Y

L

G

G

A

A

E

N

I

L

V

V

L

L

T

T

S

E

G

G

A

A

K

K

G

G

M

M

K

K

G

G

A

A

I

I

A

Q

K

K

A

A

N

E

E

E

L

I

-

V

S

A

E

S

E

D

I

P

E

Q

G

K

S

Y

F

L

I

L

P

L

G

Q

Q
|
Q

F
|
F

V

S

N

N

P

P

A

A

N

N

P

P

A

E

I

I

H

H

K

E

A

K

T

T

G

G

P

P

E

E

I

I

W

W

N

E

D

D

T

T

D

D

G

G

E

Q

V

V

D

D

I

V

F

F

I

I

A

S

G

G

V
|
V

G
|
G

T
|
T

G
|
G

G

G

T
|
T

L

L

T

T

G

G

T

V

G

T

Q

R

Y

Y

L

I

R

K

-

G

-

T

E

K

Q

G

K

K

P

T

E

D

V

L

K

I

I

T

V

V

A

A

L

V

E

E

P

P

D

T

T

D

S

S

P

P

V

V

L

I

S

A

E

Q

G

A

-

L

-

A

-

G

-

E

-

I

K

K

A

P

G

G

P

P

H

H

K

K

I

I

Q
|
Q

G
|
G

I
|
I

G

G

A
|
A

G

G

F

F

V

I

P

P

D

G

V

N

L

L

D

D

T

L

K

K

I

L

Y

I

D

D

E

K

I

V

F

V

R

G

A

I

T

T

N

N

E

E

D

E

A

A

F

I

A

S

T

T

A

A

K

R

L

R

L

L

A

M

K

E

E

E

G

G

I

I

L

L

V

A

G

G

I

I

S
|
S

S

S

G

G

A

A

S

A

L

V

H

A

A

A

I

L

E

K

Y

L

A

Q

K

E

K

D

P

E

E

S

N

F

E

T

G

N

K

K

T

N

I

I

V

V

A

V

L

I

L

L

P
|
P

D

S

T

S

G

G

D

E

R

R

Y

Y

Y

L

S

S

T

T

A

A

L

L

F

F

T

A

E

D

binding pyridoxal-5'-phosphate: K43 (= K47), N73 (= N77), V177 (= V180), G178 (= G181), T179 (= T182), G180 (= G183), T182 (= T185), G230 (= G225), S274 (= S269), P301 (= P296)
binding (1~{S},2~{S})-1-[(4-methylphenyl)methyl]-2-phenyl-cyclopropane-1-carboxylic acid: K43 (= K47), T70 (= T74), G72 (= G76), N73 (= N77), T74 (= T78), Q144 (= Q147), F145 (= F148), Q229 (= Q224), G230 (= G225), I231 (= I226), A233 (= A228)

P0A1E3 Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine sulfhydrylase A; EC 2.5.1.47 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 4 papers)
54% identity, 100% coverage: 1:310/310 of query aligns to 1:314/323 of P0A1E3

query
sites
P0A1E3

M
|
M

A

S

N

K

I

I

Y

Y

K

E

G

D

T

N

L

S

G

L

L

T

I

I

G

G

N

H

T

T

P

P

L

L

V

V

E

R

V

L

A

N

N

R

I

I

E

G

K

N

E

-

L

-

G

-

L

-

K

-

A

G

R

R

V

I

L

L

V

A

K

K

L

V

E

E

Y

S

F

R

N

N

P

P

A

S

G

F

S

S

V

V

K

K

D

C

R

R

I

I

A

G

K

A

A

N

M

M

I

I

E

W

D

D

A

A

E

E

K

K

K

R

G

G

I

V

L

L

K

K

E

P

G

G

S

V

V

E

I

L

I

V

E

E

P

P

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

G

A

L

L

A

A

S

Y

I

V

A

A

K

R

G

G

Y

Y

R

K

I

L

I

T

L

L

T

T

M

M

P

P

E

E

T

T

M

M

S

S

V

I

E

E

R

R

N

K

I

L

L

L

K

K

A

A

Y

L

G

G

A

A

E

N

I

L

V

V

L

L

T

T

S

E

G

G

A

A

K

K

G

G

M

M

K

K

G

G

A

A

I

I

A

Q

K

K

A

A

N

E

E

E

L

I

-

V

S

A

E

S

E

D

I

P

E

Q

G

K

S

Y

F

L

I

L

P

L

G

Q

Q

Q

F

F

V

S

N

N

P

P

A

A

N

N

P

P

A

E

I

I

H

H

K

E

A

K

T

T

G

G

P

P

E

E

I

I

W

W

N

E

D

D

T

T

D

D

G

G

E

Q

V

V

D

D

I

V

F

F

I

I

A

S

G

G

V

V

G

G

T

T

G

G

T

T

L

L

T

T

G

G

T

V

G

T

Q

R

Y

Y

L

I

R

K

-

G

-

T

E

K

Q

G

K

K

P

T

E

D

V

L

K

I

I

T

V

V

A

A

L

V

E

E

P

P

D

T

T

D

S

S

P

P

V

V

L

I

S

A

E

Q

G

A

-

L

-

A

-

G

-

E

-

I

K

K

A

P

G

G

P

P

H

H

K

K

I

I

Q

Q

G

G

I

I

G

G

A

A

G

G

F

F

V

I

P

P

D

G

V

N

L

L

D

D

T

L

K

K

I

L

Y

I

D

D

E

K

I

V

F

V

R

G

A

I

T

T

N

N

E

E

D

E

A

A

F

I

A

S

T

T

A

A

K

R

L

R

L

L

A

M

K

E

E

E

G

G

I

I

L

L

V

A

G

G

I

I

S
|
S

S

S

G

G

A

A

S

A

L

V

H

A

A

A

I

L

E

K

Y

L

A

Q

K

E

K

D

P

E

E

S

N

F

E

T

G

N

K

K

T

N

I

I

V

V

A

V

L

I

L

L

P

P

D

S

T

S

G

G

D

E

R

R

Y

Y

Y

L

S

S

T

T

A

A

L

L

F

F

T

A

E

D

M1 (= M1) modified: Initiator methionine, Removed
N72 (= N77) binding pyridoxal 5'-phosphate
S273 (= S269) binding pyridoxal 5'-phosphate

1d6sA Crystal structure of the k41a mutant of o-acetylserine sulfhydrylase complexed in external aldimine linkage with methionine (see paper)
53% identity, 100% coverage: 2:310/310 of query aligns to 1:313/322 of 1d6sA

query
sites
1d6sA

A

S

N

K

I

I

Y

Y

K

E

G

D

T

N

L

S

G

L

L

T

I

I

G

G

N

H

T

T

P

P

L

L

V

V

E

R

V

L

A

N

N

R

I

I

E

G

K

N

E

-

L

-

G

-

L

-

K

-

A

G

R

R

V

I

L

L

V

A

K

K

L

V

E

E

Y

S

F

R

N

N

P

P

A

S

G

F

S

S

V

V

K
x
A

D

C

R

R

I

I

A

G

K

A

A

N

M

M

I

I

E

W

D

D

A

A

E

E

K

K

K

R

G

G

I

V

L

L

K

K

E

P

G

G

S

V

V

E

I

L

I

V

E

E

P

P

T
|
T

S
x
N

G

G

N
|
N

T
|
T

G

G

I

I

G

A

L

L

A

A

S

Y

I

V

A

A

K

R

G

G

Y

Y

R

K

I

L

I

T

L

L

T

T

M

M

P

P

E

E

T

T

M

M

S

S

V

I

E

E

R

R

N

K

I

L

L

L

K

K

A

A

Y

L

G

G

A

A

E

N

I

L

V

V

L

L

T

T

S

E

G

G

A

A

K

K

G

G

M

M

K

K

G

G

A

A

I

I

A

Q

K

K

A

A

N

E

E

E

L

I

-

V

S

A

E

S

E

D

I

P

E

Q

G

K

S

Y

F

L

I

L

P

L

G

Q

Q
|
Q

F
|
F

V

S

N

N

P

P

A

A

N

N

P

P

A

E

I

I

H

H

K

E

A

K

T

T

G

G

P

P

E

E

I

I

W

W

N

E

D

D

T

T

D

D

G

G

E

Q

V

V

D

D

I

V

F

F

I

I

A

S

G

G

V

V

G
|
G

T
|
T

G
|
G

G

G

T
|
T

L

L

T

T

G

G

T

V

G

T

Q

R

Y

Y

L

I

R

K

-

G

-

T

E

K

Q

G

K

K

P

T

E

D

V

L

K

I

I

T

V

V

A

A

L

V

E

E

P

P

D

T

T

D

S

S

P

P

V

V

L

I

S

A

E

Q

G

A

-

L

-

A

-

G

-

E

-

I

K

K

A

P

G

G

P

P

H

H

K

K

I

I

Q

Q

G
|
G

I

I

G

G

A

A

G

G

F

F

V

I

P

P

D

G

V

N

L

L

D

D

T

L

K

K

I

L

Y

I

D

D

E

K

I

V

F

V

R

G

A

I

T

T

N

N

E

E

D

E

A

A

F

I

A

S

T

T

A

A

K

R

L

R

L

L

A

M

K

E

E

E

G

G

I

I

L

L

V

A

G

G

I

I

S
|
S

S

S

G

G

A

A

S

A

L

V

H

A

A

A

I

L

E

K

Y

L

A

Q

K

E

K

D

P

E

E

S

N

F

E

T

G

N

K

K

T

N

I

I

V

V

A

V

L

I

L

L

P
|
P

D

S

T

S

G

G

D

E

R

R

Y

Y

Y

L

S

S

T

T

A

A

L

L

F

F

T

A

E

D

active site: A41 (≠ K47), G228 (= G225)
binding methionine: T68 (= T74), N69 (≠ S75), N71 (= N77), T72 (= T78), Q142 (= Q147), F143 (= F148), G176 (= G181), G228 (= G225)
binding pyridoxal-5'-phosphate: N71 (= N77), G176 (= G181), T177 (= T182), G178 (= G183), T180 (= T185), G228 (= G225), S272 (= S269), P299 (= P296)

3vbeC Crystal structure of beta-cyanoalanine synthase in soybean (see paper)
51% identity, 99% coverage: 3:310/310 of query aligns to 10:316/329 of 3vbeC

query
sites
3vbeC

N

N

I

I

Y

K

K

K

G

H

T

V

L

S

G

Q

L

L

I

I

G

G

N

R

T

T

P

P

L

L

V

V

E

Y

V

L

A

N

N

K

I

V

E

T

K

E

E

-

L

-

G

G

L

C

K

G

A

A

R

Y

V

V

L

A

V

V

K

K

L

Q

E

E

Y

M

F

M

N

Q

P

P

A

T

G

A

S

S

V

I

K
|
K

D

D

R

R

I

P

A

A

K

Y

A

A

M

M

I

I

E

T

D

D

A

A

E

E

K

E

K

K

G

N

I

L

L

I

K

T

E

P

G

G

-

K

S

T

V

T

I

L

I

I

E

E

P

P

T

T

S
|
S

G

G

N
|
N

T

M

G

G

I

I

G

S

L

M

A

A

S

F

I

M

A

A

A

M

K

K

G

G

Y

Y

R

K

I

M

I

V

L

L

T

T

M

M

P

P

E

S

T

Y

M

T

S

S

V

L

E

E

R

R

N

V

I

T

L

M

K

R

A

A

Y

F

G

G

A

A

E

E

I

L

V

I

L

L

T

T

S

D

G

P

A

A

K

K

G

G

M

M

K

G

G

G

A

T

I

V

A

K

K

K

A

A

N

Y

E

E

L

L

S

L

E

E

E

N

I

T

E

P

G

N

S

A

F

H

I

M

P

L

G

Q

Q

Q

F

F

V

S

N

N

P

P

A

A

N

N

P

T

A

Q

I

V

H

H

K

F

A

E

T

T

G

G

P

P

E

E

I

I

W

W

N

E

D

D

T

T

D

N

G

G

E

Q

V

V

D

D

I

I

F

F

I

V

A
x
M

G

G

V

I

G
|
G

T
x
S

G
|
G

G

G

T
|
T

L

V

T

S

G

G

T

V

G

G

Q

Q

Y

Y

L

L

R

K

E

S

Q

K

K

N

P

P

E

N

V

V

K

K

I

I

V

Y

A

G

L

V

E
|
E

P

P

D

S

T

E

S
|
S

P

N

V

V

L

L

S

N

E

G

G

G

K

K

A

P

G

G

P

P

H

H

K

H

I

I

Q

T

G
|
G

I

N

G

G

A

V

G

G

F

F

V

K

P

P

D

D

V

I

L

L

D

D

T

L

K

D

I

V

Y

M

D

E

E

K

I

V

F

L

R

E

A

V

T

S

N

S

E

E

D

D

A

A

F

V

A

N

T

M

A

A

K

R

L

V

L

L

A

A

K

L

K

K

E

E

G

G

I

L

L

M

V

V

G

G

I

I

S
|
S

S

S

G

G

A

A

S

N

L

T

H

V

A

A

I

L

E

R

Y

L

A

A

K

Q

K

L

P

P

E

E

N

N

E

K

G

G

K

K

T

L

I

I

V

V

A

T

L

V

L

H

P
|
P

D

S

T

F

G

G

D

E

R

R

Y

Y

Y

L

S

S

T

S

A

V

L

L

F

F

T

Q

E

E

active site: K52 (= K47), S81 (= S75), E212 (= E206), S216 (= S210), S275 (= S269), P302 (= P296)
binding pyridoxal-5'-phosphate: K52 (= K47), N83 (= N77), M184 (≠ A178), G187 (= G181), S188 (≠ T182), G189 (= G183), T191 (= T185), G231 (= G225), S275 (= S269), P302 (= P296)

3vc3A Crystal structure of beta-cyanoalanine synthase k95a mutant in soybean (see paper)
50% identity, 99% coverage: 3:310/310 of query aligns to 3:309/322 of 3vc3A

query
sites
3vc3A

N

N

I

I

Y

K

K

K

G

H

T

V

L

S

G

Q

L

L

I

I

G

G

N

R

T

T

P

P

L

L

V

V

E

Y

V

L

A

N

N

K

I

V

E

T

K

E

E

-

L

-

G

G

L

C

K

G

A

A

R

Y

V

V

L

A

V

V

K

K

L

Q

E

E

Y

M

F

M

N

Q

P

P

A

T

G

A

S

S

V

I

K
x
A

D

D

R

R

I

P

A

A

K

Y

A

A

M

M

I

I

E

T

D

D

A

A

E

E

K

E

K

K

G

N

I

L

L

I

K

T

E

P

G

G

-

K

S

T

V

T

I

L

I

I

E

E

P

P

T
|
T

S
|
S

G

G

N
|
N

T
x
M

G

G

I

I

G

S

L

M

A

A

S

F

I

M

A

A

A

M

K

K

G

G

Y

Y

R

K

I

M

I

V

L

L

T

T

M

M

P

P

E

S

T

Y

M

T

S

S

V

L

E

E

R

R

N

V

I

T

L

M

K

R

A

A

Y

F

G

G

A

A

E

E

I

L

V

I

L

L

T

T

S

D

G

P

A

A

K

K

G

G

M

M

K

G

G

G

A

T

I

V

A

K

K

K

A

A

N

Y

E

E

L

L

S

L

E

E

E

N

I

T

E

P

G

N

S

A

F

H

I

M

P

L

G

Q

Q
|
Q

F

F

V

S

N

N

P

P

A

A

N

N

P

T

A

Q

I

V

H

H

K

F

A

E

T

T

G

G

P

P

E

E

I

I

W

W

N

E

D

D

T

T

D

N

G

G

E

Q

V

V

D

D

I

I

F

F

I

V

A
x
M

G

G

V

I

G
|
G

T
x
S

G
|
G

G

G

T
|
T

L

V

T

S

G

G

T

V

G

G

Q

Q

Y

Y

L

L

R

K

E

S

Q

K

K

N

P

P

E

N

V

V

K

K

I

I

V

Y

A

G

L

V

E

E

P

P

D

S

T

E

S

S

P

N

V

V

L

L

S

N

E

G

G

G

K

K

A

P

G

G

P

P

H

H

K

H

I

I

Q

T

G
|
G

I

N

G

G

A

V

G

G

F

F

V

K

P

P

D

D

V

I

L

L

D

D

T

L

K

D

I

V

Y

M

D

E

E

K

I

V

F

L

R

E

A

V

T

S

N

S

E

E

D

D

A

A

F

V

A

N

T

M

A

A

K

R

L

V

L

L

A

A

K

L

K

K

E

E

G

G

I

L

L

M

V

V

G

G

I

I

S
|
S

S

S

G

G

A

A

S

N

L

T

H

V

A

A

I

L

E

R

Y

L

A

A

K

Q

K

L

P

P

E

E

N

N

E

K

G

G

K

K

T

L

I

I

V

V

A

T

L

V

L

H

P
|
P

D

S

T

F

G

G

D

E

R

R

Y

Y

Y

L

S

S

T

S

A

V

L

L

F

F

T

Q

E

E

active site: A45 (≠ K47), S268 (= S269), P295 (= P296)
binding n-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-l-cysteine: T73 (= T74), S74 (= S75), N76 (= N77), M77 (≠ T78), Q146 (= Q147), M177 (≠ A178), G180 (= G181), S181 (≠ T182), G182 (= G183), T184 (= T185), G224 (= G225), S268 (= S269), P295 (= P296)

Query Sequence

>WP_005351659.1 NCBI__GCF_000173975.1:WP_005351659.1
MANIYKGTLGLIGNTPLVEVANIEKELGLKARVLVKLEYFNPAGSVKDRIAKAMIEDAEK
KGILKEGSVIIEPTSGNTGIGLASIAAAKGYRIILTMPETMSVERRNILKAYGAEIVLTS
GAKGMKGAIAKANELSEEIEGSFIPGQFVNPANPAIHKATTGPEIWNDTDGEVDIFIAGV
GTGGTLTGTGQYLREQKPEVKIVALEPDTSPVLSEGKAGPHKIQGIGAGFVPDVLDTKIY
DEIFRATNEDAFATAKLLAKKEGILVGISSGASLHAAIEYAKKPENEGKTIVALLPDTGD
RYYSTALFTE

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory