SitesBLAST

101 Important for activation via AroG; G→A: The catalytic efficiency and the affinity are 5 and 3-fold lower than the wild-type. The activation by AroG is 10-fold lower than the wild-type.
102 Important for activation via AroG; R→A: The catalytic efficiency and the affinity are slightly modified. The activation by AroG is 2-fold lower than the wild-type.
103 Important for activation via AroG; L→A: The catalytic efficiency and the affinity are identical to the wild-type. The activation by AroG is 10-fold lower than the wild type.

5ckxD Non-covalent complex of dahp synthase and chorismate mutase from mycobacterium tuberculosis with bound transition state analog and feedback effectors tyrosine and phenylalanine (see paper)
71% identity, 67% coverage: 21:86/98 of query aligns to 2:67/79 of 5ckxD

query
sites
5ckxD

E

E

I

I

D

D

Q

T

L

L

R

R

K

E

E

E

I

I

D

D

W

R

L

L

D

D

A

A

E

E

I

I

L

L

R

A

L

L

V

V

K

K

R

R

R
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R

A

A

E

E

V

V

S
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S

K

K

T

A

I
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I

G

G

A

K

A

A

R
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R

M

M

A

A

A

S

G

G

P

T

R

R

I

L

V

V

Y

H

N

S

R
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R

E
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E

M

M

D

K

V

V

L

I

A

E

R

R

Y

Y

R

S

E

E

L

L

G

G

P

P

E

D

G

G

R

K

Q

D

L

L

A

A

M

I

binding 8-hydroxy-2-oxa-bicyclo[3.3.1]non-6-ene-3,5-dicarboxylic acid: R24 (= R43), S28 (= S47), I31 (= I50), R35 (= R54), R47 (= R66), E48 (= E67)

Sites not aligning to the query:

binding 8-hydroxy-2-oxa-bicyclo[3.3.1]non-6-ene-3,5-dicarboxylic acid: 70

5hudE Non-covalent complex of and dahp synthase and chorismate mutase from corynebacterium glutamicum with bound transition state analog (see paper)
51% identity, 79% coverage: 21:97/98 of query aligns to 7:84/84 of 5hudE

query
sites
5hudE

E

E

I

I

D

Q

Q

K

L

Y

R

R

K

E

E

E

I

I

D

N

W

R

L

L

D

D

A

R

E

E

I

I

L

L

R

D

L

A

V

V

K

K

R

R

R
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R

A

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E

K

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S
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S

K

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T

T

I
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I

G

G

A

K

A

T

R
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R

M

M

A

S

G

G

P

T

R

R

I
x
L

V
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V

Y

H

N

T

R
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R

E
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E

M

V

D

A

V

I

L

I

A

N

R

Q

Y

F

R

R

-

E

E

E

L

I

G

G

P

E

E

E

G

G

R

P

Q

A

L

L

A

A

M

G

A

I

L

L

L
|
L

N

R

L

M

G

G

R

R

G

G

R

K

L

L

G

G

binding 8-hydroxy-2-oxa-bicyclo[3.3.1]non-6-ene-3,5-dicarboxylic acid: R29 (= R43), S33 (= S47), I36 (= I50), R40 (= R54), L48 (≠ I62), V49 (= V63), R52 (= R66), E53 (= E67), L76 (= L89)

Query Sequence

>WP_005453415.1 NCBI__GCF_000244975.1:WP_005453415.1
MNAQTHDTDEAGRSGATTAEEIDQLRKEIDWLDAEILRLVKRRAEVSKTIGAARMAAGGP
RIVYNREMDVLARYRELGPEGRQLAMALLNLGRGRLGR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory