SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_005453900.1 NCBI__GCF_000244975.1:WP_005453900.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

A0R083 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I alpha; GSI alpha; EC 6.3.1.2 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
78% identity, 100% coverage: 1:446/447 of query aligns to 1:446/446 of A0R083

query
sites
A0R083

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K363 (= K363) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

P9WN37 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I alpha; GSI alpha; EC 6.3.1.2 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
78% identity, 100% coverage: 1:446/447 of query aligns to 1:446/446 of P9WN37

query
sites
P9WN37

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K363 (= K363) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

8ooxB Glutamine synthetase (see paper)
48% identity, 99% coverage: 4:444/447 of query aligns to 2:435/438 of 8ooxB

query
sites
8ooxB

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M

V

A

E

D

W

W

D

D

N

S

Y

Y

R

R

S

T

Q

Q

V

V

T

H

P

Q

Y

W

E

E

L

L

R

D

T

R

L

Y

L

L

binding magnesium ion: E129 (= E134), E183 (= E189), E190 (= E196)

8tfkA Glutamine synthetase (see paper)
47% identity, 99% coverage: 5:447/447 of query aligns to 3:440/440 of 8tfkA

query
sites
8tfkA

Q

K

E

E

F

D

V

V

L

L

R

E

T

A

L

V

E

K

E

E

R

R

D

D

I

V

R

K

F

F

V

I

R

R

L

T

W

Q

F

F

T

T

D

D

V

T

L

L

G

G

F

I

L

I

K

K

S

S

V

W

A

A

V

I

A

P

P

A

A

E

E

Q

L

L

E

E

G

E

A

A

F

F

S

E

E

N

G

G

I

V

G

M

F

F

D

D

G

G

S

S

A

S

I

I

E

Q

G

G

F

F

A

T

R

R

V

I

Y

E

E

E

S

S

D

D

M

M

V

K

A

L

K

A

P

L

D

D

P

P

A

S

T

T

F

F

Q

R

V

I

L

L

P

P

W

W

E

R

T

P

T

A

D

T

G

G

D

A

H

-

Y

-

S

V

A

A

R

R

M

I

F

L

C

G

D

D

I

V

A

Y

M

L

P

P

D

D

G

G

S

N

P

P

S

F

W

K

A

G

D

D

P

P

R

R

H

Y

V

V

L

L

R

K

R

T

Q

A

L

I

S

K

K

E

A

A

S

E

E

K

A

M

G

G

F

F

T

S

C

M

Y

N

V

V

H

G

P

P

E
|
E

I

L

E
|
E

F

F

L

L

L

F

A

K

N

L

M

D

P

A

E

N

D

G

G

N

R

P

E

T

P

T

E

E

P

L

A

T

D

D

N

Q

G

G

Y

Y

F

F

D

D

Q

F

A

A

S

P

H

L

A

D

T

R

A

A

T

Q

H

D

F

V

R

R

H

D

A

I

I

D

E

Y

A

A

L

L

E

E

E

H

M

M

G

G

I

F

S

Q

V

I

E

E

F

A

S

S

H

H

E
|
E

A

V

A

A

P

P

G

S

Q

Q

Q

H

E
|
E

I

I

D
|
D

L
x
F

R

R

Y
x
F

A

G

D

D

A

V

L

L

T

C

M

T

A

A

D

D

N

N

V

V

M

V

T

T

F

F

R

K

Y

Y

V

V

K

K

E

S

V

I

A

A

L

Y

T

H

Q

K

G

G

V

Y

R

Y

A

A

T

S

F

F

M

M

P

P

K

K

P

P

F

L

S

F

D

G

Q

V

P

N

G
|
G

S

S

G

G

M

M

H
|
H

T

S

H
x
N

V

Q

S

S

L

L

F

F

E

K

G

D

D

G

R

K

N

N

A

V

F

F

Y

Y

S

D

P

P

E

D

D

T

P

P

Y

T

E

K

L

L

S

S

E

Q

T

D

G

A

K

M

A

Y

F

Y

V

I

A

G

G

G

L

L

H

K

H

H

A

I

R

R

E

E

I

F

S

T

A

A

V

V

T

T

N

N

Q

P

W

V

V

V

N

N

S

S

Y

Y

K

K

R
|
R

L

L

I

V

Q

P

G

G

G

Y

E
|
E

A

A

P

P

T

V

T

Y

V

I

S

S

W

W

G

S

R

A

A

Q

N

N

R
|
R

S

S

A

S

L

L

V

I

R
|
R

V

I

P

P

M

A

Y

-

S

-

P

-

G

T

K

R

A

G

S

N

S
x
G

R

T

R
|
R

V

I

E
|
E

I

L

R
|
R

T

C

P

P

D

D

S

P

A

A

C

C

N

N

P

P

Y

Y

L

L

A

A

Y

F

S

A

V

L

I

M

L

L

A

R

A

A

G

G

L

L

K

E

G

G

I

I

E

K

K

N

G

K

Y

I

E

D

L

P

P

G

P

E

A

P

A

T

E

N

D

V

N

N

I

I

W

F

T

H

L

L

S

S

D

D

A

K

E

E

R

R

K

E

A

E

A

R

G

G

Y

I

T

R

E

S

L

L

P

P

Q

A

N

D

L

L

G

K

E

E

A

A

L

I

A

D

E

E

M

M

E

K

R

G

S

S

E

K

L

F

L

V

P

K

E

E

A

A

L

L

G

G

E

E

H

H

V

V

Y

F

D

S

F

H

F

Y

L

L

R

C

N

A

K

K

R

E

V

M

E

E

W

W

D

D

N

E

Y

Y

R

K

S

A

Q

V

V

V

T

H

P

P

Y

W

E

E

L

L

R

S

T

R

L

Y

L

L

P

S

V

M

L

L

binding adenosine-5'-diphosphate: E128 (= E132), D194 (= D198), F195 (≠ L199), F197 (≠ Y201), N243 (≠ H247), R312 (= R316), R317 (= R321), G325 (≠ S332), R327 (= R334)
binding magnesium ion: E128 (= E132), E128 (= E132), E130 (= E134), E185 (= E189), E192 (= E196), E192 (= E196), H241 (= H245), E329 (= E336)
binding l-methionine-s-sulfoximine phosphate: E128 (= E132), E130 (= E134), E185 (= E189), E192 (= E196), G237 (= G241), H241 (= H245), R294 (= R298), E300 (= E304), R312 (= R316), R331 (= R338)

8ufjB Glutamine synthetase (see paper)
47% identity, 99% coverage: 5:447/447 of query aligns to 7:444/444 of 8ufjB

query
sites
8ufjB

Q

K

E

E

F

D

V

V

L

L

R

E

T

A

L

V

E

K

E

E

R

R

D

D

I

V

R

K

F

F

V

I

R

R

L

T

W

Q

F

F

T

T

D

D

V

T

L

L

G

G

F

I

L

I

K

K

S

S

V

W

A

A

V

I

A

P

P

A

A

E

E

Q

L

L

E

E

G

E

A

A

F

F

S

E

E

N

G

G

I

V

G

M

F

F

D

D

G

G

S

S

A

S

I

I

E

Q

G

G

F

F

A

T

R

R

V

I

Y

E

E

E

S

S

D

D

M

M

V

K

A

L

K

A

P

L

D

D

P

P

A

S

T

T

F

F

Q

R

V

I

L

L

P

P

W

W

E

R

T

P

T

A

D

T

G

G

D

A

H

-

Y

-

S

V

A

A

R

R

M

I

F

L

C

G

D

D

I

V

A

Y

M

L

P

P

D

D

G

G

S

N

P

P

S

F

W

K

A

G

D

D

P

P

R

R

H

Y

V

V

L

L

R

K

R

T

Q

A

L

I

S

K

K

E

A

A

S

E

E

K

A

M

G

G

F

F

T

S

C

M

Y

N

V

V

H

G

P

P

E
|
E

I

L

E
|
E

F

F

L

L

L

F

A

K

N

L

M

D

P

A

E

N

D

G

G

N

R

P

E

T

P

T

E

E

P

L

A

T

D

D

N

Q

G

G

Y

Y

F

F

D

D

Q

F

A

A

S

P

H

L

A

D

T

L

A

G

T

Q

H

D

F

V

R

R

H

D

A

I

I

D

E

Y

A

A

L

L

E

E

E

H

M

M

G

G

I

F

S

Q

V

I

E

E

F

A

S

S

H

H

E
|
E

A

V

A

A

P

P

G

S

Q

Q

Q

H

E
|
E

I

I

D

D

L

F

R

R

Y

F

A

G

D

D

A

V

L

L

T

C

M

T

A

A

D

D

N

N

V

V

M

V

T

T

F

F

R

K

Y

Y

V

V

K

K

E

S

V

I

A

A

L

Y

T

H

Q

K

G

G

V

Y

R

Y

A

A

T

S

F

F

M

M

P

P

K

K

P

P

F

L

S

F

D

G

Q

V

P

N

G

G

S

S

G

G

M

M

H
|
H

T

S

H

N

V

Q

S

S

L

L

F

F

E

K

G

D

D

G

R

K

N

N

A

V

F

F

Y

Y

S

D

P

P

E

D

D

T

P

P

Y

T

E

K

L

L

S

S

E

Q

T

D

G

A

K

M

A

Y

F

Y

V

I

A

G

G

G

L

L

H

K

H

H

A

I

R

R

E

E

I

F

S

T

A

A

V

V

T

T

N

N

Q

P

W

V

V

V

N

N

S

S

Y

Y

K

K

R

R

L

L

I

V

Q

P

G

G

G

Y

E

E

A

A

P

P

T

V

T

Y

V

I

S

S

W

W

G

S

R

A

A

Q

N

N

R

R

S

S

A

S

L

L

V

I

R

R

V

I

P

P

M

A

Y

-

S

-

P

-

G

T

K

R

A

G

S

N

S

G

R

T

R

R

V

I

E
|
E

I

L

R

R

T

C

P

P

D

D

S

P

A

A

C

C

N

N

P

P

Y

Y

L

L

A

A

Y

F

S

A

V

L

I

M

L

L

A

R

A

A

G

G

L

L

K

E

G

G

I

I

E

K

K

N

G

K

Y

I

E

D

L

P

P

G

P

E

A

P

A

T

E

N

D

V

N

N

I

I

W

F

T

H

L

L

S

S

D

D

A

K

E

E

R

R

K

E

A

E

A

R

G

G

Y

I

T

R

E

S

L

L

P

P

Q

A

N

D

L

L

G

K

E

E

A

A

L

I

A

D

E

E

M

M

E

K

R

G

S

S

E

K

L

F

L

V

P

K

E

E

A

A

L

L

G

G

E

E

H

H

V

V

Y

F

D

S

F

H

F

Y

L

L

R

C

N

A

K

K

R

E

V

M

E

E

W

W

D

D

N

E

Y

Y

R

K

S

A

Q

V

V

V

T

H

P

P

Y

W

E

E

L

L

R

S

T

R

L

Y

L

L

P

S

V

M

L

L

binding magnesium ion: E132 (= E132), E134 (= E134), E189 (= E189), E196 (= E196), H245 (= H245), E333 (= E336)

8oozA Glutamine synthetase (see paper)
48% identity, 99% coverage: 4:444/447 of query aligns to 2:427/430 of 8oozA

query
sites
8oozA

Q

K

Q

E

E

D

F

E

V

I

L

F

R

R

T

I

L

V

E

E

R

K

D

N

I

V

R

R

F

F

V

V

R

R

L

L

W

Q

F

F

T

V

D

D

V

V

L

Q

G

G

F

I

L

P

K

K

S

N

V

V

A

A

V

I

A

P

P

V

A

G

E

Q

L

L

E

E

G

K

A

A

F

L

S

G

E

P

G

G

I

I

G

H

F

F

D

D

G

G

S

S

A

S

I

I

E

E

G

G

F

-

A

-

R

-

V

-

Y

-

E

-

S

S

D

D

M

M

V

V

A

L

K

R

P

P

D

D

P

P

A

D

T

T

F

F

Q

R

V

V

L

L

P

P

W

W

E

S

T

T

T

A

D

E

G

-

D

-

H

-

Y

-

S

-

A

A

R

R

M

L

F

I

C

C

D

D

I

I

A

E

M

L

P

P

D

D

G

G

S

K

P

P

S

F

W

M

A

G

D

C

P

P

R

R

H

Q

V

V

L

L

R

K

Q

N

L

M

S

E

K

E

A

A

S

A

E

K

A

L

G

G

F

Y

T

V

C

M

Y

N

V

T

H
x
G

P

P

E
|
E

I

M

E

E

F

F

L

L

L

F

A

K

N

R

M

-

P

-

E

Q

D

D

G

G

R

M

E

P

P

T

E

N

-

I

P

P

A

Q

D

D

N

R

G

G

Y

Y

F

F

D

D

Q

L

A

A

S

P

H

I

A

D

T

L

A

A

T

E

H

E

F

I

R

K

R

R

H

E

A

I

I

V

E

L

A

V

L

L

E

E

M

M

G

G

I

F

S

E

V

V

E
|
E

F

A

S

A

H

H

E

E

A

V

A

A

P

F

G

G

Q

Q

Q

H

E

E

I

I

D

D

L
x
F

R
x
K

Y
|
Y

A

D

D

N

A

A

L

L

T

A

M

T

A

A

D

D

N

N

V

V

M

I

T

T

F

L

R

K

Y

Y

V

V

V

A

K

K

E

T

V

L

A

A

L

L

T

Q

Q

H

G

G

V

L

R

H

A

A

T

T

F

F

M

M

P

P

K

K

P

P

F

I

S

F

D

G

Q

V

P

N

G

G

S

S

G

G

M

M

H
|
H

T

T

H
x
N

V

T

S
|
S

L

L

F

F

E

K

G

D

D

G

R

K

N

N

A

A

F

F

Y

Y

S

D

P

P

E

D

D

A

P

P

Y

D

E

Q

L

I

S

S

E

D

T

T

G

L

K

R

A

Y

F

F

V

V

A

G

G

G

L

V

L

L

H

K

H

H

A

I

R

R

E

A

I

I

S

T

A

A

V

I

T

T

N

N

Q

P

W

L

V

V

N

N

S

S

Y

Y

K

K

R

R

L

L

I

V

Q

P

G

G

G

Y

E

E

A

A

P

P

T

V

T

Y

V

I

S

T

W

W

G

S

R

G

A

P

N

N

R

R

S

S

A

S

L

L

V

I

R

R

V

V

P

P

M

-

Y

-

S

A

P

P

G

-

K

R

A

G

S

N

S
|
S

R

T

R
|
R

V

I

E
|
E

I

I

R

R

T

S

P

P

D

D

S

P

A

S

C

C

N

N

P

P

Y

Y

L

L

A

A

Y

F

S

A

V

A

I

I

L

L

A

A

G

G

L

L

K

D

G

G

I

V

E

K

K

N

G

K

Y

I

E

E

L

P

P

P

P

E

A

R

A

V

E

E

D

K

N

N

I

I

W

Y

T

K

L

L

S

T

D

E

A

E

E

E

R

R

K

E

A

K

A

L

G

G

Y

I

T

G

E

M

L

L

P

P

Q

G

N

T

L

L

G

K

E

E

A

A

L

I

A

E

E

C

M

F

E

K

R

E

S

D

E

E

L

L

P

V

E

S

A

A

L

L

G

G

E

E

H

H

V

V

Y

S

D

Q

F

S

F

I

L

I

R

N

N

V

K

A

R

M

V

A

E

D

W

W

D

D

N

S

Y

Y

R

R

S

T

Q

Q

V

V

T

H

P

Q

Y

W

E

E

L

L

R

D

T

R

L

Y

L

L

binding adenosine-5'-triphosphate: G117 (≠ H130), E170 (= E184), F185 (≠ L199), K186 (≠ R200), Y187 (= Y201), N233 (≠ H247), S235 (= S249), S315 (= S332), R317 (= R334)
binding magnesium ion: E119 (= E132), H231 (= H245), E319 (= E336)

7tfaB Glutamine synthetase (see paper)
47% identity, 97% coverage: 5:439/447 of query aligns to 4:433/441 of 7tfaB

query
sites
7tfaB

Q

R

E

E

F

D

V

I

L

I

R

R

T

I

L

A

E

E

R

E

D

N

I

V

R

R

F

F

V

I

R

R

L

L

W

Q

F

F

T

T

D

D

V

L

L

L

G

G

F

T

L

I

K

K

S

N

V

V

A

E

V

I

A

P

P

V

A

S

E

Q

L

L

E

E

G

K

A

A

F

L

S

D

E

N

G

K

I

M

G

M

F

F

D

D

G

G

S

S

A

S

I

I

E

E

G

G

F
x
Y

A

V

R
|
R

V

I

Y

E

E

E

S

S

D

D

M

M

V

Y

A

L

K

Y

P

P

D

D

P

L

A

D

T

T

F

W

Q

V

V

V

L

F

P

P

W

W

E

V

T

T

T

S

D

D

G

-

D

-

H

-

Y

R

S

V

A

A

R

R

M

L

F

I

C

C

D

D

I

I

A

Y

M

K

P

P

D

D

G

G

S

S

P

P

S

F

W

A

A

G

D

D

P

P

R

R

H

G

V

I

L

L

R

K

R

R

Q

V

L

L

S

K

K

E

A

A

S

E

E

E

A

L

G

G

F

Y

T

T

C

S

Y

M

-

N

V

V

H

G

P

P

E
|
E

I

P

E
|
E

F

F

L

L

L

F

A

K

N

T

M

D

P

E

E

K

D

G

G

D

R

P

E

T

P

T

E

E

P

L

A

N

D

D

N

Q

G

G

Y
|
Y

F

F

D

D

Q

L

A

A

S

P

H

M

A

D

T

L

A

G

T

E

H

N

F

C

R

R

H

E

A

I

I

V

E

L

A

K

L

L

E

E

M

M

G

G

I

F

S

E

V

I

E

E

F

A

S

S

H

H

E
|
E

A
x
V

A

A

P

P

G

G

Q

Q

Q

H

E
|
E

I

I

D

D

L

F

R

K

Y

Y

A

A

D

D

A

A

L

V

T

K

M

A

A

A

D

D

N

Q

V

I

M

Q

T

T

F

F

R

K

Y

L

V

V

K

K

E

T

V

I

A

A

L

R

T

Q

Q

H

G

G

V

L

R

H

A

A

T

T

F

F

M

M

P

P

K

K

P

P

F

L

S

F

D

G

Q

V

P
x
N

G
|
G

S

S

G

G

M

M

H
|
H

T

C

H

N

V

Q

S

S

L

L

F

F

E

K

G

D

D

N

R

E

N

N

A

V

F

F

Y

Y

S

D

P

E

E

T

D

D

P

E

Y

L

E

G

L

L

S

S

E

Q

T

T

G

A

K

R

A

H

F

Y

V

M

A

A

G

G

L

I

L

L

H

K

H

H

A

A

R

R

E

A

I

M

S

A

A

A

V

I

T

T

N

N

Q

P

W

T

V

V

N

N

S

S

Y

Y

K

K

R
|
R

L

L

I

V

Q

P

G
|
G

G
x
Y

E
|
E

A

A

P

P

T

C

T

Y

V

V

S

A

W

W

G

S

R

A

A

S

N

N

R
|
R

S

S

A

P

L

M

V

I

R

R

V

I

P

P

M

-

Y

-

S

-

P

A

G

S

K

R

A

G

S

L

S

S

R

T

R

R

V

V

E
|
E

I

V

R

R

T

N

P

P

D

D

S

P

A

A

C

A

N

N

P

P

Y

Y

L

L

A

A

Y

L

S

A

V

V

I

M

L

L

A

R

A

A

G

G

L

L

K

D

G

G

I

I

E

K

K

R

G

Q

Y

M

E

A

L

L

P

P

P

A

A

P

A

I

E

D

D

R

N

N

I

I

W

Y

T

V

L

M

S

S

D

E

A

E

E

E

R

R

K

I

A

E

G

G

Y

I

T

P

E

S

L

L

P

P

Q

A

N

D

L

L

G

K

E

E

A

A

L

L

A

S

E

E

M

L

E

I

R

R

S

S

E

E

L

V

L

I

P

S

E

D

A

A

L

L

G

G

E

D

H

H

V

A

Y

L

D

A

F

Y

F

F

L

Y

R

E

N

L

K

K

R

E

V

I

E

E

W

W

D

D

N
x
M

Y

Y

R

R

S

T

Q

Q

V

V

T

H

P

Q

Y

W

E

E

binding glutamine: E131 (= E134), Y153 (= Y156), E186 (= E189), G238 (= G241), H242 (= H245), R295 (= R298), E301 (= E304)
binding magnesium ion: E129 (= E132), E131 (= E134), E186 (= E189), E193 (= E196), H242 (= H245), E330 (= E336)
binding : Y58 (≠ F59), R60 (= R61), V187 (≠ A190), N237 (≠ P240), G299 (= G302), Y300 (≠ G303), R313 (= R316), M424 (≠ N430)

7tdpA Structure of paenibacillus polymyxa gs bound to met-sox-p-adp (transition state complex) to 1.98 angstom (see paper)
47% identity, 97% coverage: 5:439/447 of query aligns to 4:431/439 of 7tdpA

query
sites
7tdpA

Q

R

E

E

F

D

V

I

L

I

R

R

T

I

L

A

E

E

R

E

D

N

I

V

R

R

F

F

V

I

R

R

L

L

W

Q

F

F

T

T

D

D

V

L

L

L

G

G

F

T

L

I

K

K

S

N

V

V

A

E

V

I

A

P

P

V

A

S

E

Q

L

L

E

E

G

K

A

A

F

L

S

D

E

N

G

K

I

M

G

M

F

F

D

D

G

G

S

S

A

S

I

I

E

E

G

G

F

Y

A

V

R

R

V

I

Y

E

E

E

S

S

D

D

M

M

V

Y

A

L

K

Y

P

P

D

D

P

L

A

D

T

T

F

W

Q

V

V

V

L

F

P

P

W

W

E

S

T

D

T

R

D

-

G

-

D

-

H

-

Y

-

S

V

A

A

R

R

M

L

F

I

C

C

D

D

I

I

A

Y

M

K

P

P

D

D

G

G

S

S

P

P

S

F

W

A

A

G

D

D

P

P

R

R

H

G

V

I

L

L

R

K

R

R

Q

V

L

L

S

K

K

E

A

A

S

E

E

E

A

L

G

G

F

Y

T

T

C

S

Y

M

-
x
N

V

V

H
x
G

P

P

E
|
E

I

P

E
|
E

F

F

L

L

L

F

A

K

N

T

M

D

P

E

E

K

D

G

G

D

R

P

E

T

P

T

E

E

P

L

A

N

D

D

N

Q

G

G

Y

Y

F

F

D

D

Q

L

A

A

S

P

H

M

A

D

T

L

A

G

T

E

H

N

F

C

R

R

H

E

A

I

I

V

E

L

A

K

L

L

E

E

M

M

G

G

I

F

S

E

V

I

E
|
E

F

A

S

S

H

H

E
|
E

A

V

A

A

P

P

G

G

Q

Q

Q

H

E
|
E

I

I

D
|
D

L

F

R

K

Y
|
Y

A

A

D

D

A

A

L

V

T

K

M

A

A

A

D

D

N

Q

V

I

M

Q

T

T

F

F

R

K

Y

L

V

V

K

K

E

T

V

I

A

A

L

R

T

Q

Q

H

G

G

V

L

R

H

A

A

T

T

F

F

M

M

P

P

K

K

P

P

F

L

S

F

D

G

Q

V

P

N

G
|
G

S

S

G

G

M

M

H
|
H

T

C

H
x
N

V

Q

S
|
S

L

L

F

F

E

K

G

D

D

N

R

E

N

N

A

V

F

F

Y

Y

S

D

P

E

E

T

D

D

P

E

Y

L

E

G

L

L

S

S

E

Q

T

T

G

A

K

R

A

H

F

Y

V

M

A

A

G

G

L

I

L

L

H

K

H

H

A

A

R

R

E

A

I

M

S

A

A

A

V

I

T

T

N

N

Q

P

W

T

V

V

N

N

S

S

Y

Y

K

K

R
|
R

L

L

I

V

Q

P

G

G

G

Y

E
|
E

A

A

P

P

T

C

T

Y

V

V

S

A

W

W

G

S

R

A

A

S

N

N

R
|
R

S

S

A

P

L

M

V

I

R
|
R

V

I

P

P

M

-

Y

-

S

-

P

A

G

S

K

R

A

G

S

L

S

S

R

T

R
|
R

V

V

E
|
E

I

V

R
|
R

T

N

P

P

D

D

S

P

A

A

C

A

N

N

P

P

Y

Y

L

L

A

A

Y

L

S

A

V

V

I

M

L

L

A

R

A

A

G

G

L

L

K

D

G

G

I

I

E

K

K

R

G

Q

Y

M

E

A

L

L

P

P

P

A

A

P

A

I

E

D

D

R

N

N

I

I

W

Y

T

V

L

M

S

S

D

E

A

E

E

E

R

R

K

I

A

E

G

G

Y

I

T

P

E

S

L

L

P

P

Q

A

N

D

L

L

G

K

E

E

A

A

L

L

A

S

E

E

M

L

E

I

R

R

S

S

E

E

L

V

L

I

P

S

E

D

A

A

L

L

G

G

E

D

H

H

V

A

Y

L

D

A

F

Y

F

F

L

Y

R

E

N

L

K

K

R

E

V

I

E

E

W

W

D

D

N

M

Y

Y

R

R

S

T

Q

Q

V

V

T

H

P

Q

Y

W

E

E

binding adenosine-5'-diphosphate: N123 (vs. gap), G125 (≠ H130), E127 (= E132), E179 (= E184), D193 (= D198), Y196 (= Y201), N242 (≠ H247), S244 (= S249), R316 (= R321), R326 (= R334)
binding magnesium ion: E127 (= E132), E127 (= E132), E129 (= E134), E184 (= E189), E191 (= E196), E191 (= E196), H240 (= H245), E328 (= E336)
binding l-methionine-s-sulfoximine phosphate: E127 (= E132), E129 (= E134), E184 (= E189), E191 (= E196), G236 (= G241), H240 (= H245), R293 (= R298), E299 (= E304), R311 (= R316), R330 (= R338)

P12425 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I alpha; GSI alpha; EC 6.3.1.2 from Bacillus subtilis (strain 168) (see 5 papers)
46% identity, 97% coverage: 5:439/447 of query aligns to 6:436/444 of P12425

query
sites
P12425

Q

R

E

E

F

D

V

I

L

E

R

K

T

L

L

V

E

K

E

E

R

E

D

N

I

V

R

K

F

Y

V

I

R

R

L

L

W

Q

F

F

T

T

D

D

V

I

L

L

G

G

F

T

L

I

K

K

S

N

V

V

A

E

V

I

A

P

P

V

A

S

E

Q

L

L

E

G

G

K

A

A

F

L

S

D

E

N

G

K

I

V

G

M

F

F

D

D

G

G

S

S

A

S

I

I

E

E

G
|
G

F

F

A

V

R
|
R

V

I

Y

E

E

E

S

S

D

D

M

M

V

Y

A

L

K

Y

P

P

D

D

P

L

A

N

T

T

F

F

Q

V

V

I

L

F

P

P

W

W

E

T

T

A

T

E

D

K

G

G

D

K

H

-

Y

-

S

V

A

A

R

R

M

F

F

I

C

C

D

D

I

I

A

Y

M

N

P

P

D

D

G

G

S

T

P

P

S

F

W

E

A

G

D

D

P

P

R

R

H

N

V

N

L

L

R

K

R

R

Q

I

L

L

S

K

K

E

A

M

S

E

E

D

A

L

G

G

F

F

T

S

C

D

Y

F

-

N

V

L

H

G

P

P

E
|
E

I

P

E
|
E

F

F

L

L

L

F

A

-

N

K

M

L

P

D

E

E

D

K

G

G

R

-

E

E

P

P

-

T

-

L

E

E

P

L

A

N

D

D

N

K

G

G

Y

Y

F

F

D

D

Q

L

A

A

S

P

H

T

A

D

T

L

A

G

T

E

H

N

F

C

R

R

H

D

A

I

I

V

E

L

A

E

L

L

E

E

M

M

G

G

I

F

S

E

V

I

E

E

F

A

S

S

H

H

E
|
E

A
x
V

A

A

P

P

G

G

Q

Q

Q

H

E
|
E

I

I

D

D

L

F

R

K

Y

Y

A

A

D

G

A

A

L

V

T

R

M

S

A

C

D

D

N

D

V

I

M

Q

T

T

F

F

R

K

Y

L

V

V

K

K

E

T

V

I

A

A

L

R

T

K

Q

H

G

G

V

L

R

H

A

A

T

T

F

F

M

M

P

P

K

K

P

P

F

L

S

F

D

G

Q

V

P

N

G
|
G

S

S

G

G

M

M

H
|
H

T

C

H

N

V

L

S

S

L

L

F

F

E

K

G

N

D

G

R

V

N

N

A

A

F

F

Y

F

S

D

P

E

E

N

D

A

P

D

Y

L

E

Q

L

L

S

S

E

E

T

T

G

A

K

K

A

H

F

F

V

I

A

A

G

G

L

I

L

V

H

K

H

H

A

A

R

T

E

S

I

F

S

T

A

A

V

V

T

T

N

N

Q

P

W

T

V

V

N

N

S

S

Y

Y

K

K

R

R

L

L

I

V

Q

P

G
|
G

G

Y

E
|
E

A

A

P
|
P

T

C

T

Y

V

V

S

A

W

W

G

S

R

A

A

Q

N

N

R

R

S

S

A

P

L

L

V

I

R

R

V

I

P

P

M

-

Y

-

S

-

P

A

G

S

K

R

A

G

S

I

S

S

R

T

R

R

V

V

E
|
E

I

V

R

R

T

S

P

V

D

D

S

P

A

A

C

A

N

N

P

P

Y

Y

L

L

A

A

Y

L

S

S

V

V

I

L

L

L

A

A

G

G

L

L

K

D

G

G

I

I

E

K

K

N

G

K

Y

L

E

E

L

A

P

P

P

A

A

P

A

I

E

D

D

R

N

N

I

I

W

Y

T

V

L

M

S

S

D

K

A

E

E

E

R

R

K

M

A

E

A

N

G

G

Y

I

T

V

E

D

L

L

P

P

Q

A

N

T

L

L

G

A

E

E

A

A

L

L

A

E

E

E

M

F

E

K

R

S

S

N

E

E

L

V

L

M

P

V

E

K

A

A

L

L

G

G

E

E

H

H

V

L

Y

F

D

E

F

H

F

F

L

I

R

E

N

A

K

K

R

E

V

I

E
|
E

W

W

D

D

N

M

Y

F

R

R

S

T

Q

Q

V

V

T

H

P

P

Y

W

E

E

G59 (= G58) mutation to R: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant derepresses amtB-lacZ fusion and glnRA-lacZ fusion.
R62 (= R61) Important for inhibition by glutamine; mutation to A: Highly resistant to inhibition by glutamine and AMP. Regulation by TnrA and GlnR is abolished. Only small differences (less than 2-fold) in its steady-state kinetic constants compared with the wild-type. Similar sensitivity to Met-Sox that compared to the wild-ytpe.
E132 (= E132) binding Mg(2+)
E134 (= E134) binding Mg(2+)
E189 (= E189) binding Mg(2+)
V190 (≠ A190) mutation to A: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant partially relieves expression of the glnRA-lacZ fusion, but has no effect on the TnrA-dependent regulation of amtB-lacZ fusion. Resistant to inhibition by MetSox.
E196 (= E196) binding Mg(2+)
G241 (= G241) binding L-glutamate
H245 (= H245) binding Mg(2+)
G302 (= G302) mutation to E: Unable to form stable complex with TnrA. In the presence of glutamine, amtB-lacZ fusion is only 4-fold regulated by TnrA, whereas glnRA-lacZ fusion is derepressed. This mutant retains enzymatic specific activity with a 2-fold decrease of the affinity for glutamate and glutamine compared to the wild-type. Slightly less sensitive to inhibition by glutamine.
E304 (= E304) mutation to A: Highly resistant to Met-Sox inhibition. 8- and 2-fold increase of the affinity for glutamate and ATP, respectively. Strong decrease of the affinity for ammonium.
P306 (= P306) mutation to H: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant completely derepresses glnRA-lacZ fusion, whereas amtB-lacZ fusion expression is only partially derepresses.
E333 (= E336) binding Mg(2+)
E424 (= E427) mutation to K: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant derepresses amtB-lacZ fusion and glnRA-lacZ fusion. Although it is defective in regulation, this mutant retains enzymatic specific activity and similar affinity for ATP, glutamate and glutamine compared to the wild-type. Slightly less sensitive to inhibition by glutamine.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

4lnkA B. Subtilis glutamine synthetase structures reveal large active site conformational changes and basis for isoenzyme specific regulation: structure of gs-glutamate-amppcp complex (see paper)
46% identity, 97% coverage: 5:439/447 of query aligns to 5:435/443 of 4lnkA

query
sites
4lnkA

Q

R

E

E

F

D

V

I

L

E

R

K

T

L

L

V

E

K

E

E

R

E

D

N

I

V

R

K

F

Y

V

I

R

R

L

L

W

Q

F

F

T

T

D

D

V

I

L

L

G

G

F

T

L

I

K

K

S

N

V

V

A

E

V

I

A

P

P

V

A

S

E

Q

L

L

E

G

G
x
K

A

A

F

L

S

D

E

N

G

K

I

V

G
x
M

F

F

D
|
D

G

G

S

S

A

S

I

I

E

E

G

G

F

F

A

V

R

R

V

I

Y

E

E

E

S

S

D

D

M

M

V

Y

A

L

K

Y

P

P

D

D

P

L

A

N

T

T

F

F

Q

V

V

I

L

F

P

P

W

W

E

T

T

A

T

E

D

K

G

G

D

K

H

-

Y

-

S

V

A

A

R

R

M

F

F

I

C

C

D

D

I

I

A

Y

M

N

P

P

D

D

G

G

S

T

P

P

S

F

W

E

A

G

D

D

P

P

R

R

H

N

V

N

L

L

R

K

R

R

Q

I

L

L

S

K

K

E

A

M

S

E

E

D

A

L

G

G

F

F

T

S

C

D

Y

F

-

N

V

L

H

G

P

P

E
|
E

I

P

E
|
E

F

F

L

L

L

F

A

-

N

K

M

L

P

D

E

E

D

K

G

G

R

-

E

E

P

P

-

T

-

L

E

E

P

L

A

N

D

D

N

K

G

G

Y

Y

F

F

D

D

Q

L

A

A

S

P

H

T

A

D

T

L

A

G

T

E

H

N

F

C

R

R

H

D

A

I

I

V

E

L

A

E

L

L

E

E

M

M

G

G

I

F

S

E

V

I

E

E

F

A

S

S

H

H

E
|
E

A
x
V

A

A

P

P

G

G

Q

Q

Q

H

E
|
E

I

I

D

D

L
x
F

R

K

Y
|
Y

A

A

D

G

A

A

L

V

T

R

M

S

A

C

D

D

N

D

V

I

M

Q

T

T

F

F

R

K

Y

L

V

V

K

K

E

T

V

I

A

A

L

R

T

K

Q

H

G

G

V

L

R

H

A

A

T

T

F

F

M

M

P

P

K

K

P

P

F

L

S

F

D

G

Q

V

P
x
N

G
|
G

S

S

G
|
G

M

M

H
|
H

T

C

H
x
N

V

L

S
|
S

L

L

F

F

E

K

G

N

D

G

R

V

N

N

A

A

F

F

Y

F

S

D

P

E

E

N

D

A

P

D

Y

L

E

Q

L

L

S

S

E

E

T

T

G

A

K

K

A

H

F

F

V

I

A

A

G

G

L

I

L

V

H

K

H

H

A

A

R

T

E

S

I

F

S

T

A

A

V

V

T

T

N

N

Q

P

W

T

V

V

N

N

S

S

Y

Y

K

K

R

R

L

L

I

V

Q

P

G

G

G

Y

E
|
E

A

A

P

P

T

C

T

Y

V

V

S

A

W

W

G

S

R

A

A

Q

N

N

R
|
R

S

S

A

P

L

L

V

I

R

R

V

I

P

P

M

-

Y

-

S

-

P

A

G
x
S

K

R

A

G

S

I

S
|
S

R

T

R
|
R

V

V

E
|
E

I

V

R
|
R

T

S

P

V

D

D

S

P

A

A

C

A

N

N

P

P

Y

Y

L

L

A

A

Y

L

S

S

V

V

I

L

L

L

A

A

G

G

L

L

K

D

G

G

I

I

E

K

K

N

G

K

Y

L

E

E

L

A

P

P

P

A

A

P

A

I

E

D

D

R

N

N

I

I

W

Y

T

V

L

M

S

S

D

K

A

E

E

E

R

R

K

M

A

E

A

N

G

G

Y

I

T

V

E

D

L

L

P

P

Q

A

N

T

L

L

G

A

E

E

A

A

L

L

A

E

E

E

M

F

E

K

R

S

S

N

E

E

L

V

L

M

P

V

E

K

A

A

L

L

G

G

E

E

H

H

V

L

Y

F

D

E

F

H

F

F

L

I

R

E

N

A

K

K

R

E

V

I

E

E

W

W

D

D

N

M

Y

F

R

R

S

T

Q

Q

V

V

T

H

P

P

Y

W

E

E

active site: D52 (= D52), E131 (= E132), E133 (= E134), E188 (= E189), E195 (= E196), H244 (= H245), R315 (= R316), E332 (= E336), R334 (= R338)
binding adenosine-5'-diphosphate: K43 (≠ G43), M50 (≠ G50), F198 (≠ L199), Y200 (= Y201), N246 (≠ H247), S248 (= S249), S324 (≠ G328), S328 (= S332), R330 (= R334)
binding glutamic acid: E133 (= E134), E188 (= E189), V189 (≠ A190), N239 (≠ P240), G240 (= G241), G242 (= G243), E303 (= E304)
binding magnesium ion: E131 (= E132), E188 (= E189), E195 (= E196), H244 (= H245), E332 (= E336)

4lniA B. Subtilis glutamine synthetase structures reveal large active site conformational changes and basis for isoenzyme specific regulation: structure of the transition state complex (see paper)
46% identity, 97% coverage: 5:439/447 of query aligns to 5:435/443 of 4lniA

query
sites
4lniA

Q

R

E

E

F

D

V

I

L

E

R

K

T

L

L

V

E

K

E

E

R

E

D

N

I

V

R

K

F

Y

V

I

R

R

L

L

W

Q

F

F

T

T

D

D

V

I

L

L

G

G

F

T

L

I

K

K

S

N

V

V

A

E

V

I

A

P

P

V

A

S

E

Q

L

L

E

G

G

K

A

A

F

L

S

D

E

N

G

K

I

V

G

M

F

F

D
|
D

G

G

S

S

A

S

I

I

E

E

G

G

F

F

A

V

R

R

V

I

Y

E

E

E

S

S

D

D

M

M

V

Y

A

L

K

Y

P

P

D

D

P

L

A

N

T

T

F

F

Q

V

V

I

L

F

P

P

W

W

E

T

T

A

T

E

D

K

G

G

D

K

H

-

Y

-

S

V

A

A

R

R

M

F

F

I

C

C

D

D

I

I

A

Y

M

N

P

P

D

D

G

G

S

T

P

P

S

F

W

E

A

G

D

D

P

P

R

R

H

N

V

N

L

L

R

K

R

R

Q

I

L

L

S

K

K

E

A

M

S

E

E

D

A

L

G

G

F

F

T

S

C

D

Y

F

-

N

V

L

H

G

P

P

E
|
E

I

P

E
|
E

F

F

L

L

L

F

A

-

N

K

M

L

P

D

E

E

D

K

G

G

R

-

E

E

P

P

-

T

-

L

E

E

P

L

A

N

D

D

N

K

G

G

Y

Y

F

F

D

D

Q

L

A

A

S

P

H

T

A

D

T

L

A

G

T

E

H

N

F

C

R

R

H

D

A

I

I

V

E

L

A

E

L

L

E

E

M

M

G

G

I

F

S

E

V

I

E
|
E

F

A

S

S

H

H

E
|
E

A

V

A

A

P

P

G

G

Q

Q

Q

H

E
|
E

I

I

D
|
D

L

F

R

K

Y
|
Y

A

A

D

G

A

A

L

V

T

R

M

S

A

C

D

D

N

D

V

I

M

Q

T

T

F

F

R

K

Y

L

V

V

K

K

E

T

V

I

A

A

L

R

T

K

Q

H

G

G

V

L

R

H

A

A

T

T

F

F

M

M

P

P

K

K

P

P

F

L

S

F

D

G

Q

V

P

N

G

G

S

S

G

G

M

M

H
|
H

T

C

H
x
N

V

L

S
|
S

L

L

F

F

E

K

G

N

D

G

R

V

N

N

A

A

F

F

Y

F

S

D

P

E

E

N

D

A

P

D

Y

L

E

Q

L

L

S

S

E

E

T

T

G

A

K

K

A

H

F

F

V

I

A

A

G

G

L

I

L

V

H

K

H

H

A

A

R

T

E

S

I

F

S

T

A

A

V

V

T

T

N

N

Q

P

W

T

V

V

N

N

S

S

Y

Y

K

K

R
|
R

L

L

I

V

Q

P

G

G

G

Y

E
|
E

A

A

P

P

T

C

T

Y

V

V

S

A

W

W

G

S

R

A

A

Q

N

N

R
|
R

S

S

A

P

L

L

V

I

R
|
R

V

I

P

P

M

-

Y

-

S

-

P

A

G

S

K

R

A

G

S

I

S

S

R

T

R
|
R

V

V

E
|
E

I

V

R
|
R

T

S

P

V

D

D

S

P

A

A

C

A

N

N

P

P

Y

Y

L

L

A

A

Y

L

S

S

V

V

I

L

L

L

A

A

G

G

L

L

K

D

G

G

I

I

E

K

K

N

G

K

Y

L

E

E

L

A

P

P

P

A

A

P

A

I

E

D

D

R

N

N

I

I

W

Y

T

V

L

M

S

S

D

K

A

E

E

E

R

R

K

M

A

E

A

N

G

G

Y

I

T

V

E

D

L

L

P

P

Q

A

N

T

L

L

G

A

E

E

A

A

L

L

A

E

E

E

M

F

E

K

R

S

S

N

E

E

L

V

L

M

P

V

E

K

A

A

L

L

G

G

E

E

H

H

V

L

Y

F

D

E

F

H

F

F

L

I

R

E

N

A

K

K

R

E

V

I

E

E

W

W

D

D

N

M

Y

F

R

R

S

T

Q

Q

V

V

T

H

P

P

Y

W

E

E

active site: D52 (= D52), E131 (= E132), E133 (= E134), E188 (= E189), E195 (= E196), H244 (= H245), R315 (= R316), E332 (= E336), R334 (= R338)
binding adenosine-5'-diphosphate: E131 (= E132), E183 (= E184), D197 (= D198), Y200 (= Y201), N246 (≠ H247), S248 (= S249), R320 (= R321), R330 (= R334)
binding magnesium ion: E131 (= E132), E131 (= E132), E133 (= E134), E188 (= E189), E195 (= E196), E195 (= E196), H244 (= H245), E332 (= E336)
binding l-methionine-s-sulfoximine phosphate: E133 (= E134), E188 (= E189), H244 (= H245), R297 (= R298), E303 (= E304), R315 (= R316), R334 (= R338)

8oooA Glutamine synthetase from methanothermococcus thermolithotrophicus in complex with 2-oxoglutarate and mgatp at 2.15 a resolution (see paper)
44% identity, 99% coverage: 6:446/447 of query aligns to 4:447/447 of 8oooA

query
sites
8oooA

E

E

F

Q

V

V

L

L

R

E

T

Y

L

V

E

K

E

S

R

N

D

N

I

V

R

K

F
|
F

V

M

R
|
R

L

F

W

Q

F

F

T

V

D

D

V

I

L

L

G

G

F

V

L

P

K

K

S

N

V

V

A
|
A

V

F

-

P

-

I

-

K

-

A

-

G

-

E

-

K

A

G

P

I

A

E

E

E

L

L

E

R

G

D

A

V

F

L

S

E

E

N

G

G

I

L

G

Y

F

F

D

D

G

G

S

S

A

S

I

I

E

E

G

G

F

F

A

V

R

G

V

I

Y

N

E

E

S

S

D

D

M

M

V

M

A

L

K

K

P

P

D

D

P

L

A

S

T

T

F

F

Q

S

V

V

L

L

P

P

W

W

E
x
R

T

P

T

S

D

E

G

K

D

S

H

-

Y

-

S
x
V

A

A

R

R

M

V

F

I

C

C

D

D

I

V

A

Y

M

T

P

T

D

K

G

G

S

K

P

P

S

F

W

E

A

G

D

D

P

P

R

R

H

G

V

C

L

L

R

K

R

R

Q

V

L

M

S

E

K

E

-

F

A

K

S

K

E

E

A

F

G

N

F

G

T

E

C

Y

Y

F

V

V

H

G

P

P

E
|
E

I

P

E

E

F

F

L

L

A

K

N

K

M

D

P

P

E

H

D

N

G

P

R

H

E

K

P

Y

E

I

P

P

A

A

D

D

N

D

G

G

Y

Y

F

F

D

D

Q

L

A

E

S

P

H

M

A

D

T

E

A

A

T
x
P

H

D

F

I

R
|
R

H

D

A

I

I

V

E

F

A

A

L

L

E

E

E

N

M

L

G

G

I

F

S

H

V

V

E
|
E

F

A

S
|
S

H

H

E

E

A

V

A

A

P

P

G

G

Q

Q

Q

H

E

E

I

V

D

D

L
x
F

R
x
K

Y
x
F

A

D

D

D

A

A

L

L

T

K

M

T

A

A

D

D

N

S

V

V

M

I

T

T

F

F

R

K

Y

T

V

T

V

I

K

K

E

T

V

I

A

A

L

E

T

Q

Q

H

G

G

V

L

R

K

A

A

T

T

F

F

M

M

P

P

K

K

P

P

F

F

S

F

D

G

Q

M

P

N

G

G

S

S

G

G

M

M

H

H

T

C

H
|
H

V

Q

S
|
S

L

I

F

W

E

L

G

N

D

G

R

E

N

P

A

S

F

F

Y

Y

S

D

P

E

E

N

D

A

P

P

Y

Y

E

Q

L

L

S

S

E

E

T

T

G

C

K

M

A

N

F

Y

V

V

A

A

G

G

L

I

L

L

H

K

H

H

A

A

R

K

E

A

I

I

S

V

A

A

V

I

T

T

N

N

Q

P

W

T

V

V

N

N

S

S

Y

Y

K

K

R

R

L

L

I

V

Q

P

G

G

G

Y

E

E

A

A

P

P

T

V

T

N

V

I

S

A

W

W

G

A

R

N

A

S

N

N

R

R

S

S

A

A

L

I

V

I

R
|
R

V

V

P

P

M

A

Y

-

S

-

P

-

G

A

K

R

A

G

S

K

S

G

R

T

R
|
R

V

I

E

E

I

F

R

R

T

A

P

P

D

D

S

P

A

S

C

C

N

N

P

P

Y

Y

L

L

A

A

Y

F

S

T

V

V

I

M

L

L

A

A

G

G

L

L

K

D

G

G

I

V

E

K

K

N

G

K

Y

L

E

D

L

A

P

P

P

E

A

P

A

V

E

E

D

R

N

N

I

I

W

F

T

A

L

M

S

S

D

E

A

A

E

E

R

K

K

K

A

E

A

L

G

G

Y

I

T

E

E

S

L

V

P

P

Q

A

N

N

L

L

G

K

E

A

A

A

L

L

A

D

E

E

M

L

E

E

R

N

S

N

E

D

L

V

L

L

P

K

E

N

A

A

L

L

G

G

E

K

H

H

V

I

Y

F

D

E

F

S

F

F

L

L

R

E

N

I

K

K

R

N

V

A

E

E

W

W

D

D

N

S

Y

F

R

R

S

T

Q

S

V

V

T

T

P

D

Y

W

E

E

L

T

R

T

T

A

L

Y

L

L

P

K

V

I

binding 2-oxoglutaric acid: F17 (= F19), R19 (= R21), A33 (= A35), R87 (≠ E82), V93 (≠ S90), P170 (≠ T166), R173 (= R169), R174 (= R170), S190 (= S186)
binding adenosine-5'-triphosphate: E136 (= E132), E188 (= E184), F203 (≠ L199), K204 (≠ R200), F205 (≠ Y201), H251 (= H247), S253 (= S249), R325 (= R321), R335 (= R334)

8ooqB Glutamine synthetase from Methanothermococcus thermolithotrophicus (see paper)
44% identity, 99% coverage: 6:446/447 of query aligns to 3:446/446 of 8ooqB

query
sites
8ooqB

E

E

F

Q

V

V

L

L

R

E

T

Y

L

V

E

K

E

S

R

N

D

N

I

V

R

K

F
|
F

V

M

R
|
R

L

F

W

Q

F

F

T

V

D

D

V

I

L

L

G

G

F

V

L

P

K

K

S

N

V

V

A
|
A

V

F

-

P

-

I

-

K

-

A

-

G

-

E

-

K

A

G

P

I

A

E

E

E

L

L

E

R

G

D

A

V

F

L

S

E

E

N

G

G

I

L

G

Y

F

F

D

D

G

G

S

S

A

S

I

I

E

E

G

G

F

F

A

V

R

G

V

I

Y

N

E

E

S

S

D

D

M

M

V

M

A

L

K

K

P

P

D

D

P

L

A

S

T

T

F

F

Q

S

V

V

L

L

P

P

W

W

E
x
R

T

P

T

S

D

E

G

K

D

S

H

-

Y

-

S
x
V

A

A

R

R

M

V

F

I

C

C

D

D

I

V

A

Y

M

T

P

T

D

K

G

G

S

K

P

P

S

F

W

E

A

G

D

D

P

P

R

R

H

G

V

C

L

L

R

K

R

R

Q

V

L

M

S

E

K

E

-

F

A

K

S

K

E

E

A

F

G

N

F

G

T

E

C

Y

Y

F

V

V

H

G

P

P

E

E

I

P

E
|
E

F

F

L

L

A

K

N

K

M

D

P

P

E

H

D

N

G

P

R

H

E

K

P

Y

E

I

P

P

A

A

D

D

N

D

G

G

Y

Y

F

F

D

D

Q

L

A

E

S

P

H

M

A

D

T

E

A

A

T
x
P

H

D

F

I

R
|
R

H

D

A

I

I

V

E

F

A

A

L

L

E

E

E

N

M

L

G

G

I

F

S

H

V

V

E

E

F

A

S
|
S

H

H

E
|
E

A

V

A

A

P

P

G

G

Q

Q

Q

H

E
|
E

I

V

D

D

L

F

R

K

Y

F

A

D

D

D

A

A

L

L

T

K

M

T

A

A

D

D

N

S

V

V

M

I

T

T

F

F

R

K

Y

T

V

T

V

I

K

K

E

T

V

I

A

A

L

E

T

Q

Q

H

G

G

V

L

R

K

A

A

T

T

F

F

M

M

P

P

K

K

P

P

F

F

S

F

D

G

Q

M

P

N

G

G

S

S

G

G

M

M

H

H

T

C

H

H

V

Q

S

S

L

I

F

W

E

L

G

N

D

G

R

E

N

P

A

S

F

F

Y

Y

S

D

P

E

E

N

D

A

P

P

Y

Y

E

Q

L

L

S

S

E

E

T

T

G

C

K

M

A

N

F

Y

V

V

A

A

G

G

L

I

L

L

H

K

H

H

A

A

R

K

E

A

I

I

S

V

A

A

V

I

T

T

N

N

Q

P

W

T

V

V

N

N

S

S

Y

Y

K

K

R

R

L

L

I

V

Q

P

G

G

G

Y

E

E

A

A

P

P

T

V

T

N

V

I

S

A

W

W

G

A

R

N

A

S

N

N

R

R

S

S

A

A

L

I

V

I

R

R

V

V

P

P

M

A

Y

-

S

-

P

-

G

A

K

R

A

G

S

K

S

G

R

T

R

R

V

I

E

E

I

F

R

R

T

A

P

P

D

D

S

P

A

S

C

C

N

N

P

P

Y

Y

L

L

A

A

Y

F

S

T

V

V

I

M

L

L

A

A

G

G

L

L

K

D

G

G

I

V

E

K

K

N

G

K

Y

L

E

D

L

A

P

P

P

E

A

P

A

V

E

E

D

R

N

N

I

I

W

F

T

A

L

M

S

S

D

E

A

A

E

E

R

K

K

K

A

E

A

L

G

G

Y

I

T

E

E

S

L

V

P

P

Q

A

N

N

L

L

G

K

E

A

A

A

L

L

A

D

E

E

M

L

E

E

R

N

S

N

E

D

L

V

L

L

P

K

E

N

A

A

L

L

G

G

E

K

H

H

V

I

Y

F

D

E

F

S

F

F

L

L

R

E

N

I

K

K

R

N

V

A

E

E

W

W

D

D

N

S

Y

F

R

R

S

T

Q

S

V

V

T

T

P

D

Y

W

E

E

L

T

R

T

T

A

L

Y

L

L

P

K

V

I

binding 2-oxoglutaric acid: F16 (= F19), R18 (= R21), A32 (= A35), R86 (≠ E82), V92 (≠ S90), P169 (≠ T166), R172 (= R169), R173 (= R170), S189 (= S186)
binding magnesium ion: E137 (= E134), E192 (= E189), E199 (= E196)

4s0rD Structure of gs-tnra complex (see paper)
46% identity, 97% coverage: 5:439/447 of query aligns to 9:439/447 of 4s0rD

query
sites
4s0rD

Q

R

E

E

F

D

V

I

L

E

R

K

T

L

L

V

E

K

E

E

R

E

D

N

I

V

R

K

F

Y

V

I

R

R

L

L

W

Q

F

F

T

T

D

D

V

I

L

L

G

G

F

T

L

I

K

K

S

N

V

V

A

E

V

I

A

P

P

V

A

S

E

Q

L

L

E

G

G

K

A

A

F

L

S

D

E

N

G

K

I

V

G

M

F

F

D
|
D

G

G

S

S

A

S

I

I

E

E

G

G

F
|
F

A
x
V

R
|
R

V
x
I

Y

E

E

E

S

S

D

D

M

M

V

Y

A

L

K

Y

P

P

D

D

P

L

A

N

T

T

F

F

Q

V

V

I

L

F

P

P

W

W

E

T

T

A

T

E

D

K

G

G

D

K

H

-

Y

-

S

V

A

A

R

R

M

F

F

I

C

C

D

D

I

I

A

Y

M

N

P

P

D

D

G

G

S

T

P

P

S

F

W

E

A

G

D

D

P

P

R

R

H

N

V

N

L

L

R

K

R

R

Q

I

L

L

S

K

K

E

A

M

S

E

E

D

A

L

G

G

F

F

T

S

C

D

Y

F

-

N

V

L

H

G

P

P

E
|
E

I

P

E
|
E

F

F

L

L

L

F

A

-

N

K

M

L

P

D

E

E

D

K

G

G

R

-

E

E

P

P

-

T

-

L

E

E

P

L

A

N

D

D

N

K

G

G

Y

Y

F

F

D
|
D

Q

L

A

A

S

P

H

T

A

D

T

L

A

G

T

E

H

N

F

C

R

R

H

D

A

I

I

V

E

L

A

E

L

L

E

E

M

M

G

G

I

F

S

E

V

I

E

E

F

A

S

S

H

H

E
|
E

A

V

A

A

P

P

G

G

Q

Q

Q

H

E
|
E

I

I

D

D

L

F

R

K

Y

Y

A

A

D

G

A

A

L

V

T

R

M

S

A

C

D

D

N

D

V

I

M

Q

T

T

F

F

R

K

Y

L

V

V

K

K

E

T

V

I

A

A

L

R

T

K

Q

H

G

G

V

L

R

H

A

A

T

T

F

F

M

M

P

P

K

K

P

P

F

L

S

F

D
x
G

Q
x
V

P
x
N

G

G

S

S

G

G

M

M

H
|
H

T

C

H

N

V

L

S

S

L

L

F

F

E

K

G

N

D

G

R

V

N

N

A

A

F

F

Y

F

S

D

P

E

E

N

D

A

P

D

Y

L

E

Q

L

L

S

S

E

E

T

T

G

A

K

K

A

H

F

F

V

I

A

A

G

G

L

I

L

V

H

K

H

H

A

A

R

T

E

S

I

F

S

T

A

A

V

V

T

T

N

N

Q

P

W

T

V

V

N

N

S

S

Y

Y

K

K

R
|
R

L

L

I

V

Q

P

G
|
G

G
x
Y

E
|
E

A

A

P

P

T

C

T

Y

V

V

S

A

W

W

G

S

R

A

A

Q

N

N

R
|
R

S

S

A

P

L

L

V

I

R

R

V

I

P

P

M

-

Y

-

S

-

P

A

G

S

K

R

A

G

S

I

S

S

R

T

R

R

V

V

E
|
E

I

V

R
|
R

T

S

P

V

D

D

S

P

A

A

C

A

N

N

P

P

Y

Y

L

L

A

A

Y

L

S

S

V

V

I

L

L

L

A

A

G

G

L

L

K

D

G

G

I

I

E

K

K

N

G

K

Y

L

E

E

L

A

P

P

P

A

A

P

A

I

E

D

D

R

N

N

I

I

W
x
Y

T

V

L

M

S

S

D

K

A

E

E

E

R

R

K

M

A

E

A

N

G

G

Y

I

T

V

E

D

L

L

P

P

Q

A

N

T

L

L

G

A

E

E

A

A

L

L

A

E

E

E

M

F

E

K

R

S

S

N

E

E

L

V

L

M

P

V

E

K

A

A

L

L

G

G

E

E

H

H

V

L

Y

F

D

E

F
x
H

F

F

L

I

R

E

N

A

K

K

R

E

V
x
I

E

E

W

W

D

D

N
x
M

Y

F

R

R

S

T

Q

Q

V

V

T

H

P

P

Y

W

E

E

active site: D56 (= D52), E135 (= E132), E137 (= E134), E192 (= E189), E199 (= E196), H248 (= H245), R319 (= R316), E336 (= E336), R338 (= R338)
binding glutamine: E137 (= E134), E192 (= E189), R301 (= R298), E307 (= E304)
binding magnesium ion: I66 (≠ V62), E135 (= E132), E135 (= E132), E199 (= E196), H248 (= H245), H248 (= H245), E336 (= E336), H419 (≠ F419)
binding : F63 (= F59), V64 (≠ A60), R65 (= R61), I66 (≠ V62), D161 (= D158), G241 (≠ D238), V242 (≠ Q239), N243 (≠ P240), G305 (= G302), Y306 (≠ G303), Y376 (≠ W376), I426 (≠ V426), M430 (≠ N430)

7tdvC Glutamine synthetase (see paper)
45% identity, 97% coverage: 5:439/447 of query aligns to 5:435/443 of 7tdvC

query
sites
7tdvC

Q

K

E

E

F

D

V

I

L

R

R

K

T

F

L

V

E

E

R

E

D

N

I

V

R

R

F

Y

V

L

R

R

L

L

W

Q

F

F

T

T

D

D

V

I

L

L

G

G

F

T

L

I

K

K

S

N

V

V

A

E

V

V

A

P

P

V

A

S

E

Q

L

L

E

E

G

K

A

V

F

L

S

D

E

N

G

E

I

M

G

M

F

F

D

D

G

G

S

S

A

S

I

I

E

E

G

G

F

F

A

V

R

R

V

I

Y

E

E

E

S

S

D

D

M

M

V

Y

A

L

K

H

P

P

D

D

P

L

A

D

T

T

F

W

Q

V

V

I

L

F

P

P

W

W

E

-

T

-

T

T

D

A

G

G

D

Q

H

G

Y

K

S

V

A

A

R

R

M

L

F

I

C

C

D

D

I

V

A

Y

M

K

P

T

D

D

G

G

S

T

P

P

S

F

W

E

A

G

D

D

P

P

R

R

H

A

V

N

L

L

R

K

R

R

Q

V

L

L

S

K

K

E

A

M

S

E

E

D

A

L

G

G

F

F

T

T

C

D

Y

F

-

N

V

L

H
x
G

P

P

E
|
E

I

P

E
|
E

F

F

L

L

L

F

A

K

N

-

M

L

P

D

E

E

D

K

G

G

R

-

E

E

P

P

-

T

-

L

E

E

P

L

A

N

D

D

N

D

G

G

Y

Y

F

F

D

D

Q

L

A

A

S

P

H

T

A

D

T

L

A

G

T

E

H

N

F

C

R

R

H

D

A

I

I

V

E

L

A

E

L

L

E

E

E

D

M

M

G

G

I

F

S

D

V

I

E
|
E

F

A

S

S

H

H

E
|
E

A

V

A

A

P

P

G

G

Q

Q

Q

H

E
|
E

I

I

D
|
D

L
x
F

R
x
K

Y
|
Y

A

A

D

D

A

A

L

V

T

T

M

A

A

C

D

D

N

N

V

I

M

Q

T

T

F

F

R

K

Y

L

V

V

K

K

E

T

V

I

A

A

L

R

T

K

Q

H

G

N

V

L

R

H

A

A

T

T

F

F

M

M

P

P

K

K

P

P

F

L

S

F

D

G

Q

V

P

N

G
|
G

S

S

G

G

M

M

H
|
H

T

F

H
x
N

V
|
V

S
|
S

L

L

F

F

E

K

G

G

D

K

R

E

N

N

A

A

F

F

Y

F

S

D

P

P

E

N

D

T

P

E

Y

M

E

G

L

L

S

T

E

E

T

T

G

A

K

Y

A

Q

F

F

V

T

A

A

G

G

L

V

L

L

H

K

H

N

A

A

R

R

E

G

I

F

S

T

A

A

V

V

T

C

N

N

Q

P

W

L

V

V

N

N

S

S

Y

Y

K

K

R
|
R

L

L

I

V

Q

P

G

G

G

Y

E
|
E

A

A

P

P

T

C

T

Y

V

I

S

A

W

W

G

S

R

G

A

K

N

N

R
|
R

S

S

A

P

L

L

V

I

R
|
R

V

V

P

P

M

-

Y

-

S

-

P

S

G

S

K

R

A

G

S

L

S
|
S

R

T

R
|
R

V

I

E
|
E

I

V

R

R

T

S

P

V

D

D

S

P

A

A

C

A

N

N

P

P

Y

Y

L

M

A

A

Y

L

S

A

V

A

I

I

L

L

A

E

A

A

G

G

L

L

K

D

G

G

I

I

E

K

K

N

G

K

Y

L

E

K

L

V

P

P

P

E

A

P

A

V

E

N

D

Q

N

N

I

I

W

Y

T

E

L

M

S

N

D

R

A

E

E

E

R

R

K

E

A

A

A

V

G

G

Y

I

T

Q

E

D

L

L

P

P

Q

S

N

T

L

L

G

Y

E

T

A

A

L

L

A

K

E

A

M

M

E

R

R

E

S

N

E

E

L

V

L

I

P

K

E

K

A

A

L

L

G

G

E

N

H

H

V

I

Y

Y

D

N

F

Q

F

F

L

I

R

N

N

S

K

K

R

S

V

I

E

E

W

W

D

D

N

Y

Y

Y

R

R

S

T

Q

Q

V

V

T

S

P

E

Y

W

E

E

binding adenosine-5'-diphosphate: G129 (≠ H130), E131 (= E132), E183 (= E184), D197 (= D198), F198 (≠ L199), K199 (≠ R200), Y200 (= Y201), N246 (≠ H247), V247 (= V248), S248 (= S249), R320 (= R321), S328 (= S332), R330 (= R334)
binding magnesium ion: E131 (= E132), E131 (= E132), E133 (= E134), E188 (= E189), E195 (= E196), E195 (= E196), H244 (= H245), E332 (= E336)
binding l-methionine-s-sulfoximine phosphate: E131 (= E132), E133 (= E134), E188 (= E189), E195 (= E196), G240 (= G241), H244 (= H245), R297 (= R298), E303 (= E304), R315 (= R316)

7tf9S L. Monocytogenes gs(14)-q-glnr peptide (see paper)
45% identity, 99% coverage: 5:446/447 of query aligns to 5:442/443 of 7tf9S

query
sites
7tf9S

Q

K

E

E

F

D

V

I

L

F

R

R

T

F

L

A

E

D

E

E

R

Q

D

N

I

V

R

K

F

F

V

I

R

R

L

L

W

Q

F

F

T

T

D

D

V

I

L

L

G

G

F

I

L

I

K

K

S

N

V

V

A

E

V

I

A

P

P

V

A

S

E

Q

L

L

E

K

G

K

A

A

F

L

S

D

E

N

G

K

I

I

G

M

F

F

D

D

G

G

S

S

A

S

I

I

E

E

G

G

F
|
F

A
x
V

R

R

V

I

Y

E

E

E

S

S

D

D

M

M

V

Y

A

L

K

F

P

P

D

D

P

L

A

D

T

T

F

W

Q

V

V

V

L

F

P

P

W

W

E

T

T

A

T

E

D

K

G

G

D

K

H

-

Y

-

S

V

A

A

R

R

M

M

F

I

C

C

D

D

I

I

A

Y

M

N

P

P

D

D

G

M

S

T

P

P

S

F

W

A

A

G

D

D

P

P

R

R

H

A

V

N

L

L

R

K

R

R

Q

V

L

L

S

K

K

E

A

M

S

E

E

E

A

L

G

G

F

F

T

T

C

E

Y

F

-

N

V

L

H

G

P

P

E
|
E

I

P

E
|
E

F

F

L

L

L

F

A

K

N

-

M

-

P

L

E

D

D

E

G

N

R

R

E

R

P

P

-

T

-

L

E

E

P

L

A

N

D

D

N

S

G

G

Y
|
Y

F

F

D

D

Q

L

A

A

S

P

H

T

A

D

T

L

A

G

T

E

H

N

F

C

R

R

H

D

A

I

I

V

E

L

A

E

L

L

E

E

M

M

G

G

I

F

S

E

V

I

E

E

F

A

S

S

H

H

E
|
E

A

V

A

A

P

P

G

G

Q

Q

Q

H

E
|
E

I

I

D

D

L

F

R

K

Y

Y

A

E

D

D

A

A

L

I

T

T

M

A

A

C

D

D

N

S

V

I

M

Q

T

T

F

F

R

K

Y

L

V

V

K

K

E

T

V

I

A

A

L

R

T

K

Q

H

G

G

V

L

R

H

A

A

T

T

F

F

M

M

P

P

K

K

P

P

F

L

S

F

D

G

Q

V

P

N

G
|
G

S

S

G
|
G

M

M

H
|
H

T

F

H

N

V

M

S

S

L

L

F

F

E

N

G

E

D

K

R

G

N

N

A

A

F

F

Y

F

S

D

P

E

E

S

D

G

P

E

Y

L

E

E

L

L

S

S

E

Q

T

T

G

A

K

Y

A

H

F

F

V

L

A

A

G

G

L

M

L

L

H

K

H

H

A

A

R

R

E

G

I

Y

S

T

A

A

V

V

T

T

N

N

Q

P

W

T

V

I

N

N

S

S

Y

F

K

K

R
|
R

L

L

I

V

Q

P

G

G

G

Y

E
|
E

A

A

P

P

T

C

T

Y

V

I

S

A

W

W

G

S

R

G

A

K

N

N

R

R

S

S

A

P

L

L

V

V

R

R

V

V

P

P

M

-

Y

-

S

-

P

S

G

S

K

R

A

G

S

L

S

S

R

T

R

R

V

L

E
|
E

I

L

R

R

T

S

P

V

D

D

S

P

A

S

C

A

N

N

P

P

Y

Y

L

L

A

A

Y

M

S

A

V

V

I

L

L

L

A

K

A

A

G

G

L

L

K

S

G

G

I

I

E

K

K

D

G

E

Y

L

E

T

L

P

P

P

P

A

A

P

A

V

E

D

D

R

N

N

I

I

W

Y

T

G

L

M

S

N

D

E

A

E

E

E

R

R

K

E

A

A

A

T

G

G

Y

I

T

Y

E

D

L

L

P

P

Q

E

N

S

L

L

G

G

E

H

A

A

L

L

A

I

E

E

M

L

E

E

R

K

S

N

E

E

L

I

P

K

E

D

A

G

L

L

G

G

E

E

H

H

V

I

Y

F

D

E

F

H

F

F

L

I

R
x
E

N

A

K

K

R

T

V
x
I

E

E

W

C

D

D

N
x
M

Y

F

R

R

S

T

Q

A

V

V

T

H

P

P

Y

W

E

E

L

R

R

E

T

Q

L

Y

L

L

P

E

V

I

binding glutamine: E133 (= E134), Y155 (= Y156), E188 (= E189), G240 (= G241), G242 (= G243), R297 (= R298), E303 (= E304)
binding magnesium ion: E131 (= E132), E133 (= E134), E188 (= E189), E195 (= E196), H244 (= H245), E332 (= E336)
binding : F59 (= F59), V60 (≠ A60), E418 (≠ R422), I422 (≠ V426), M426 (≠ N430)

7tenA Glutamine synthetase (see paper)
45% identity, 99% coverage: 5:446/447 of query aligns to 4:441/442 of 7tenA

query
sites
7tenA

Q

K

E

E

F

D

V

I

L

F

R

R

T

F

L

A

E

D

E

E

R

Q

D

N

I

V

R

K

F

F

V

I

R

R

L

L

W

Q

F

F

T

T

D

D

V

I

L

L

G

G

F

I

L

I

K

K

S

N

V

V

A

E

V

I

A

P

P

V

A

S

E

Q

L

L

E

K

G

K

A

A

F

L

S

D

E

N

G

K

I

I

G

M

F

F

D

D

G

G

S

S

A

S

I

I

E

E

G

G

F

F

A

V

R

R

V

I

Y

E

E

E

S

S

D

D

M

M

V

Y

A

L

K

F

P

P

D

D

P

L

A

D

T

T

F

W

Q

V

V

V

L

F

P

P

W

W

E

T

T

A

T

E

D

K

G

G

D

K

H

-

Y

-

S

V

A

A

R

R

M

M

F

I

C

C

D

D

I

I

A

Y

M

N

P

P

D

D

G

M

S

T

P

P

S

F

W

A

A

G

D

D

P

P

R

R

H

A

V

N

L

L

R

K

R

R

Q

V

L

L

S

K

K

E

A

M

S

E

E

E

A

L

G

G

F

F

T

T

C

E

Y

F

-

N

V

L

H
x
G

P

P

E
|
E

I

P

E
|
E

F

F

L

L

L

F

A

K

N

-

M

-

P

L

E

D

D

E

G

N

R

R

E

R

P

P

-

T

-

L

E

E

P

L

A

N

D

D

N

S

G

G

Y

Y

F

F

D

D

Q

L

A

A

S

P

H

T

A

D

T

L

A

G

T

E

H

N

F

C

R

R

H

D

A

I

I

V

E

L

A

E

L

L

E

E

M

M

G

G

I

F

S

E

V

I

E
|
E

F

A

S

S

H

H

E
|
E

A

V

A

A

P

P

G

G

Q

Q

Q

H

E
|
E

I

I

D
|
D

L
x
F

R
x
K

Y
|
Y

A

E

D

D

A

A

L

I

T

T

M

A

A

C

D

D

N

S

V

I

M

Q

T

T

F

F

R

K

Y

L

V

V

K

K

E

T

V

I

A

A

L

R

T

K

Q

H

G

G

V

L

R

H

A

A

T

T

F

F

M

M

P

P

K

K

P

P

F

L

S

F

D

G

Q

V

P
x
N

G
|
G

S

S

G

G

M

M

H
|
H

T

F

H
x
N

V

M

S
|
S

L

L

F

F

E

N

G

E

D

K

R

G

N

N

A

A

F

F

Y

F

S

D

P

E

E

S

D

G

P

E

Y

L

E

E

L

L

S

S

E

Q

T

T

G

A

K

Y

A

H

F

F

V

L

A

A

G

G

L

M

L

L

H

K

H

H

A

A

R

R

E

G

I

Y

S

T

A

A

V

V

T

T

N

N

Q

P

W

T

V

I

N

N

S

S

Y

F

K

K

R
|
R

L

L

I

V

Q

P

G

G

G

Y

E
|
E

A

A

P

P

T

C

T

Y

V

I

S

A

W

W

G

S

R

G

A

K

N

N

R
|
R

S

S

A

P

L

L

V

V

R
|
R

V

V

P

P

M

-

Y

-

S

-

P

S

G

S

K

R

A

G

S

L

S
|
S

R

T

R
|
R

V

L

E

E

I

L

R
|
R

T

S

P

V

D

D

S

P

A

S

C

A

N

N

P

P

Y

Y

L

L

A

A

Y

M

S

A

V

V

I

L

L

L

A

K

A

A

G

G

L

L

K

S

G

G

I

I

E

K

K

D

G

E

Y

L

E

T

L

P

P

P

P

A

A

P

A

V

E

D

D

R

N

N

I

I

W

Y

T

G

L

M

S

N

D

E

A

E

E

E

R

R

K

E

A

A

A

T

G

G

Y

I

T

Y

E

D

L

L

P

P

Q

E

N

S

L

L

G

G

E

H

A

A

L

L

A

I

E

E

M

L

E

E

R

K

S

N

E

E

L

I

P

K

E

D

A

G

L

L

G

G

E

E

H

H

V

I

Y

F

D

E

F

H

F

F

L

I

R

E

N

A

K

K

R

T

V

I

E

E

W

C

D

D

N

M

Y

F

R

R

S

T

Q

A

V

V

T

H

P

P

Y

W

E

E

L

R

R

E

T

Q

L

Y

L

L

P

E

V

I

binding adenosine-5'-diphosphate: G128 (≠ H130), E130 (= E132), E182 (= E184), D196 (= D198), F197 (≠ L199), K198 (≠ R200), Y199 (= Y201), N245 (≠ H247), S247 (= S249), R319 (= R321), S327 (= S332), R329 (= R334)
binding l-methionine-s-sulfoximine phosphate: E130 (= E132), E132 (= E134), E187 (= E189), E194 (= E196), N238 (≠ P240), G239 (= G241), H243 (= H245), R296 (= R298), E302 (= E304), R314 (= R316), R333 (= R338)

7tf6A Glutamine synthetase (see paper)
45% identity, 97% coverage: 5:439/447 of query aligns to 4:430/438 of 7tf6A

query
sites
7tf6A

Q

K

E

E

F

D

V

I

L

R

R

K

T

F

L

A

E

E

R

E

D

N

I

V

R

R

F

Y

V

L

R

R

L

L

W

Q

F

F

T

T

D

D

V

I

L

L

G

G

F

T

L

I

K

K

S

N

V

V

A

E

V

V

A

P

P

V

A

S

E

Q

L

L

E

E

G

K

A

V

F

L

S

D

E

N

G

E

I

M

G

M

F

F

D

D

G

G

S

S

A

S

I

I

E

E

G

G

F
|
F

A

V

R
|
R

V

I

Y

E

E

E

S

S

D

D

M

M

V

Y

A

L

K

H

P

P

D

D

P

L

A

D

T

T

F

W

Q

V

V

I

L

F

P

P

W

W

E

G

T

K

T

V

D

-

G

-

D

-

H

-

Y

-

S

-

A

A

R

R

M

L

F

I

C

C

D

D

I

V

A

Y

M

K

P

T

D

D

G

G

S

T

P

P

S

F

W

E

A

G

D

D

P

P

R

R

H

A

V

N

L

L

R

K

R

R

Q

V

L

L

S

K

K

E

A

M

S

E

E

D

A

L

G

G

F

F

T

T

C

D

Y

F

-

N

V

L

H

G

P

P

E
|
E

I

P

E
|
E

F

F

L

L

L

F

A

K

N

-

M

L

P

D

E

E

D

K

G

G

R

-

E

E

P

P

-

T

-

L

E

E

P

L

A

N

D

D

N

D

G

G

Y

Y

F

F

D

D

Q

L

A

A

S

P

H

T

A

D

T

L

A

G

T

E

H

N

F

C

R

R

H

D

A

I

I

V

E

L

A

E

L

L

E

E

E

D

M

M

G

G

I

F

S

D

V

I

E

E

F

A

S

S

H

H

E
|
E

A

V

A

A

P

P

G

G

Q

Q

Q

H

E
|
E

I

I

D

D

L

F

R

K

Y

Y

A

A

D

D

A

A

L

V

T

T

M

A

A

C

D

D

N

N

V

I

M

Q

T

T

F

F

R

K

Y

L

V

V

K

K

E

T

V

I

A

A

L

R

T

K

Q

H

G

N

V

L

R

H

A

A

T

T

F

F

M

M

P

P

K

K

P

P

F

L

S

F

D
x
G

Q

V

P
x
N

G
|
G

S

S

G

G

M

M

H
|
H

T

F

H

N

V

V

S

S

L

L

F

F

E

K

G

G

D

K

R

E

N

N

A

A

F

F

Y

F

S

D

P

P

E

N

D

T

P

E

Y

M

E

G

L

L

S

T

E

E

T

T

G

A

K

Y

A

Q

F

F

V

T

A

A

G

G

L

V

L

L

H

K

H

N

A

A

R

R

E

G

I

F

S

T

A

A

V

V

T

C

N

N

Q

P

W

L

V

V

N

N

S

S

Y

Y

K

K

R
|
R

L

L

I

V

Q

P

G
|
G

G
x
Y

E
|
E

A

A

P

P

T

C

T

Y

V

I

S

A

W

W

G

S

R

G

A

K

N

N

R
|
R

S

S

A

P

L

L

V

I

R

R

V

V

P

P

M

-

Y

-

S

-

P

S

G

S

K

R

A

G

S

L

S

S

R

T

R

R

V

I

E
|
E

I

V

R

R

T

S

P

V

D

D

S

P

A

A

C

A

N

N

P

P

Y

Y

L

M

A

A

Y

L

S

A

V

A

I

I

L

L

A

E

A

A

G

G

L

L

K

D

G

G

I

I

E

K

K

N

G

K

Y

L

E

K

L

V

P

P

P

E

A

P

A

V

E

N

D

Q

N

N

I

I

W
x
Y

T

E

L

M

S

N

D

R

A

E

E

E

R

R

K

E

A

A

A

V

G

G

Y

I

T

Q

E

D

L

L

P

P

Q

S

N

T

L

L

G

Y

E

T

A

A

L

L

A

K

E

A

M

M

E

R

R

E

S

N

E

E

L

V

L

I

P

K

E

K

A

A

L

L

G

G

E

N

H

H

V

I

Y

Y

D

N

F

Q

F

F

L

I

R

N

N

S

K

K

R

S

V

I

E

E

W

W

D

D

N
x
Y

Y

Y

R

R

S

T

Q

Q

V

V

T

S

P

E

Y

W

E

E

binding glutamine: E128 (= E134), E183 (= E189), G235 (= G241), H239 (= H245), R292 (= R298), E298 (= E304)
binding magnesium ion: E126 (= E132), E128 (= E134), E183 (= E189), E190 (= E196), H239 (= H245), E327 (= E336)
binding : F58 (= F59), R60 (= R61), G232 (≠ D238), N234 (≠ P240), G296 (= G302), Y297 (≠ G303), R310 (= R316), Y367 (≠ W376), Y421 (≠ N430)

Sites not aligning to the query:

binding : 433, 437

5zlpJ Crystal structure of glutamine synthetase from helicobacter pylori (see paper)
34% identity, 87% coverage: 12:402/447 of query aligns to 16:430/478 of 5zlpJ

query
sites
5zlpJ

L

C

E

K

E

E

R

N

D

E

I

V

R

E

F

F

V

V

R

D

L

F

W

R

F

F

T

S

D

D

V

I

L

K

G

G

F

T

L

W

K

N

S

H

V

I

A

A

V

Y

A

S

P

F

A

G

E

A

L

L

-

T

E

H

G

G

A

M

F

L

S

K

E

E

G

G

I

I

G

P

F

F

D

D

G

A

S

S

A

C

I

F

E

K

G

G

F

W

A

Q

R

G

V

I

Y

E

E

H

S

S

D

D

M

M

V

I

A

L

K

T

P

P

D

D

P

L

A

V

T

R

F

Y

Q

F

V

I

L

D

P

P

W

F

E

S

T

A

T

D

D

-

G

-

D

-

H

-

Y

V

S

S

A

V

R

V

M

V

F

F

C

C

D

D

I

V

A

Y

-

D

M

V

P

Y

D

K

G

N

S

Q

P

P

S

Y

W

E

A

K

D

C

P

P

R

R

H

S

V

I

L

A

R

K

Q

A

L

L

S

Q

K

H

A

L

S

K

E

D

A

S

G

G

F

L

-

G

-

D

T

V

C

A

Y
|
Y

V

F

H

G

P

A

E
|
E

I

N

E
|
E

F

F

L

I

L

F

A

D

N

S

M

I

P

K

-

I

-

K

-

D

-

A

-

S

-

N

-

S

-

Q

-

Y

-

E

E

V

D

D

G

S

R

E

E

E

P

G

E

E

-

W

-

N

-

R

-

D

-

R

-

S

-

F

-

E

-

N

-

G

-

V

-

N

-

F

-

G

-

H

-

R

P

P

A

G

D

K

N

Q

G

G

Y

Y

F

M

D

P

Q

V

A

P

S

P

H

T

A

D

T

T

A

M

T

M

H

D

F

I

R

R

R

T

H

E

A

I

I

V

E

K

A

V

L

L

E

N

E

Q

M

V

G

G

I

L

S

E

V

T

E
x
F

F

V

S

V

H

H

E
|
E

A

V

A

A

P

Q

G

A

Q

Q

Q

G

E
|
E

I

V

D

G

L

V

R

K

Y
x
F

A

G

D

D

A

L

L

V

T

E

M

A

A

A

D

D

N

N

V

V

M

Q

T

K

F

L

R

K

Y

Y

V

V

K

K

E

M

V

V

A

A

L

H

T

L

Q

N

G

G

V

K

R

T

A

A

T

T

F

F

M

M

P

P

K

K

P

P

F

L

S

Y

D

G

Q

D

P

N

G
|
G

S

S

G

G

M

M

H
|
H

T

T

H
|
H

V

V

S
|
S

L

V

F

W

E

K

G

N

D

N

R

E

N

N

A

L

F

F

Y

-

S

S

P

G

E

E

D

T

P

Y

Y

K

E

G

L

L

S

S

E

E

T

F

G

A

K

L

A

H

F

F

V

L

A

G

G

G

L

V

L

L

H

R

H

H

A

A

R

R

E

G

I

L

S

A

A

A

V

F

T

T

N

N

Q

A

W

S

V

T

N

N

S

S

Y

Y

K

K

R

R

L

L

I

I

Q

P

G

G

G

Y

E
|
E

A

A

P

P

T

S

T

I

V

L

S

T

W

Y

G

S

R

A

A

N

N

N

R
|
R

S

S

A

A

L

S

V

V

R
|
R

V

I

P

P

M

-

Y

Y

S

G

P

I

G

S

K

K

A

N

S

S

S

A

R

-

R
|
R

V

F

E

E

I

F

R
|
R

T

F

P

P

D

D

S

S

A

S

C

S

N

N

P

P

Y

Y

L

L

A

A

Y

F

S

A

V

A

I

I

L

L

A

M

A

A

G

G

L

M

K

D

G

G

I

V

E

K

K

N

G

K

Y

I

E

D

L

P

P

G

P

E

A

A

A

M

E

D

D

I

N

N

I

L

W

F

T

K

L

L

S

T

D

L

A

D

E

E

R

I

K

R

A

E

A

K

G

G

Y

I

T

K

E

Q

L

M

P

P

Q

H

N

T

L

L

G

R

E

R

A

S

L

L

A

E

E

E

M

M

binding adenosine-5'-diphosphate: Y132 (= Y128), E136 (= E132), F215 (≠ E184), F232 (≠ Y201), H278 (= H247), S280 (= S249), R351 (= R321), R362 (= R334)
binding magnesium ion: E138 (= E134), E220 (= E189), E227 (= E196)
binding phosphinothricin: E138 (= E134), E220 (= E189), G272 (= G241), H276 (= H245), E334 (= E304), R346 (= R316), R366 (= R338)

5zlpL Crystal structure of glutamine synthetase from helicobacter pylori (see paper)
34% identity, 87% coverage: 12:402/447 of query aligns to 14:428/476 of 5zlpL

query
sites
5zlpL

L

C

E

K

E

E

R

N

D

E

I

V

R

E

F

F

V

V

R

D

L

F

W

R

F

F

T

S

D

D

V

I

L

K

G

G

F

T

L

W

K

N

S

H

V

I

A

A

V

Y

A

S

P

F

A

G

E

A

L

L

-

T

E

H

G

G

A

M

F

L

S

K

E

E

G

G

I

I

G

P

F

F

D

D

G

A

S

S

A

C

I

F

E

K

G

G

F

W

A

Q

R

G

V

I

Y

E

E

H

S

S

D

D

M

M

V

I

A

L

K

T

P

P

D

D

P

L

A

V

T

R

F

Y

Q

F

V

I

L

D

P

P

W

F

E

S

T

A

T

D

D

-

G

-

D

-

H

-

Y

V

S

S

A

V

R

V

M

V

F

F

C

C

D

D

I

V

A

Y

-

D

M

V

P

Y

D

K

G

N

S

Q

P

P

S

Y

W

E

A

K

D

C

P

P

R

R

H

S

V

I

L

A

R

K

Q

A

L

L

S

Q

K

H

A

L

S

K

E

D

A

S

G

G

F

L

-

G

-

D

T

V

C

A

Y

Y

V

F

H
x
G

P

A

E
|
E

I

N

E
|
E

F

F

L

I

L

F

A

D

N

S

M

I

P

K

-

I

-

K

-

D

-

A

-

S

-

N

-

S

-

Q

-

Y

-

E

E

V

D

D

G

S

R

E

E

E

P

G

E

E

-

W

-

N

-

R

-

D

-

R

-

S

-

F

-

E

-

N

-

G

-

V

-

N

-

F

-

G

-

H

-

R

P

P

A

G

D

K

N

Q

G

G

Y

Y

F

M

D

P

Q

V

A

P

S

P

H

T

A

D

T

T

A

M

T

M

H

D

F

I

R

R

R

T

H

E

A

I

I

V

E

K

A

V

L

L

E

N

E

Q

M

V

G

G

I

L

S

E

V

T

E
x
F

F

V

S

V

H

H

E
|
E

A

V

A

A

P

Q

G

A

Q

Q

Q

G

E
|
E

I

V

D

G

L

V

R

K

Y
x
F

A

G

D

D

A

L

L

V

T

E

M

A

A

A

D

D

N

N

V

V

M

Q

T

K

F

L

R

K

Y

Y

V

V

K

K

E

M

V

V

A

A

L

H

T

L

Q

N

G

G

V

K

R

T

A

A

T

T

F

F

M

M

P

P

K

K

P

P

F

L

S

Y

D

G

Q

D

P

N

G
|
G

S

S

G

G

M

M

H
|
H

T

T

H
|
H

V

V

S
|
S

L

V

F

W

E

K

G

N

D

N

R

E

N

N

A

L

F

F

Y

-

S

S

P

G

E

E

D

T

P

Y

Y

K

E

G

L

L

S

S

E

E

T

F

G

A

K

L

A

H

F

F

V

L

A

G

G

G

L

V

L

L

H

R

H

H

A

A

R

R

E

G

I

L

S

A

A

A

V

F

T

T

N

N

Q

A

W

S

V

T

N

N

S

S

Y

Y

K

K

R

R

L

L

I

I

Q

P

G

G

G

Y

E
|
E

A

A

P

P

T

S

T

I

V

L

S

T

W

Y

G

S

R

A

A

N

N

N

R
|
R

S

S

A

A

L

S

V

V

R
|
R

V

I

P

P

M

-

Y

Y

S

G

P

I

G

S

K

K

A

N

S

S

S

A

R

-

R
|
R

V

F

E

E

I

F

R
|
R

T

F

P

P

D

D

S

S

A

S

C

S

N

N

P

P

Y

Y

L

L

A

A

Y

F

S

A

V

A

I

I

L

L

A

M

A

A

G

G

L

M

K

D

G

G

I

V

E

K

K

N

G

K

Y

I

E

D

L

P

P

G

P

E

A

A

A

M

E

D

D

I

N

N

I

L

W

F

T

K

L

L

S

T

D

L

A

D

E

E

R

I

K

R

A

E

A

K

G

G

Y

I

T

K

E

Q

L

M

P

P

Q

H

N

T

L

L

G

R

E

R

A

S

L

L

A

E

E

E

M

M

binding adenosine-5'-diphosphate: G132 (≠ H130), E134 (= E132), F213 (≠ E184), F230 (≠ Y201), H276 (= H247), S278 (= S249), R349 (= R321), R360 (= R334)
binding magnesium ion: E136 (= E134), E218 (= E189), E225 (= E196)
binding (2s)-2-amino-4-[methyl(phosphonooxy)phosphoryl]butanoic acid: E136 (= E134), E218 (= E189), G270 (= G241), H274 (= H245), E332 (= E304), R344 (= R316), R364 (= R338)

Query Sequence

>WP_005453900.1 NCBI__GCF_000244975.1:WP_005453900.1
MDRQQEFVLRTLEERDIRFVRLWFTDVLGFLKSVAVAPAELEGAFSEGIGFDGSAIEGFA
RVYESDMVAKPDPATFQVLPWETTDGDHYSARMFCDIAMPDGSPSWADPRHVLRRQLSKA
SEAGFTCYVHPEIEFFLLANMPEDGREPEPADNGGYFDQASHATATHFRRHAIEALEEMG
ISVEFSHHEAAPGQQEIDLRYADALTMADNVMTFRYVVKEVALTQGVRATFMPKPFSDQP
GSGMHTHVSLFEGDRNAFYSPEDPYELSETGKAFVAGLLHHAREISAVTNQWVNSYKRLI
QGGEAPTTVSWGRANRSALVRVPMYSPGKASSRRVEIRTPDSACNPYLAYSVILAAGLKG
IEKGYELPPAAEDNIWTLSDAERKAAGYTELPQNLGEALAEMERSELLPEALGEHVYDFF
LRNKRVEWDNYRSQVTPYELRTLLPVL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory