SitesBLAST
Comparing WP_005456986.1 NCBI__GCF_000244975.1:WP_005456986.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
P45744 Isochorismate synthase DhbC; Isochorismate mutase; EC 5.4.4.2 from Bacillus subtilis (strain 168) (see paper)
49% identity, 96% coverage: 14:399/401 of query aligns to 11:397/398 of P45744
- S271 (≠ E270) modified: Phosphoserine
P0AEJ2 Isochorismate synthase EntC; Isochorismate mutase; EC 5.4.4.2 from Escherichia coli (strain K12) (see paper)
38% identity, 93% coverage: 24:396/401 of query aligns to 18:389/391 of P0AEJ2
- T140 (≠ I138) binding Mg(2+)
- T142 (≠ D140) binding Mg(2+)
- V145 (≠ L143) binding Mg(2+)
- D146 (≠ E144) binding Mg(2+)
- G214 (= G217) binding isochorismate
- S215 (= S218) binding isochorismate
- E241 (= E244) binding isochorismate; binding Mg(2+)
- A303 (= A310) binding isochorismate; mutation to T: Loss of mutase activity.
- L304 (≠ V311) mutation to A: Loss of mutase activity.
- F327 (≠ Y334) mutation to Y: Loss of mutase activity.
- I346 (= I353) mutation to L: Loss of mutase activity.
- R347 (= R354) binding isochorismate
- F359 (= F366) mutation to Q: Loss of mutase activity.
- G361 (= G368) binding isochorismate
- E376 (= E383) binding Mg(2+)
- K380 (= K387) binding isochorismate
3hwoA Crystal structure of escherichia coli enterobactin-specific isochorismate synthase entc in complex with isochorismate (see paper)
38% identity, 93% coverage: 24:396/401 of query aligns to 6:377/379 of 3hwoA
- active site: K135 (= K145), E185 (= E200), A201 (= A216), E229 (= E244), H264 (= H279), A291 (= A310), F315 (≠ Y334), R335 (= R354), G351 (= G370), E364 (= E383), K368 (= K387)
- binding (5S,6S)-5-[(1-carboxyethenyl)oxy]-6-hydroxycyclohexa-1,3-diene-1-carboxylic acid: G202 (= G217), S203 (= S218), E229 (= E244), H264 (= H279), I334 (= I353), R335 (= R354), A348 (= A367), G349 (= G368), E364 (= E383), K368 (= K387)
- binding magnesium ion: T128 (≠ I138), T130 (≠ D140), V133 (≠ L143), D134 (≠ E144), E229 (= E244), E364 (= E383)
5jy8A An iron-bound structure of the isochorismate synthase entc (see paper)
38% identity, 93% coverage: 24:396/401 of query aligns to 3:367/368 of 5jy8A
- active site: K125 (= K145), E175 (= E200), A191 (= A216), E219 (= E244), H254 (= H279), A281 (= A310), F305 (≠ Y334), R325 (= R354), G341 (= G370), E354 (= E383), K358 (= K387)
- binding fe (iii) ion: E219 (= E244), E237 (= E262), H239 (≠ D264), E354 (= E383)
- binding (3R,4R)-3-[(1-carboxyethenyl)oxy]-4-hydroxycyclohexa-1,5-diene-1-carboxylic acid: E175 (= E200), L190 (= L215), A191 (= A216), G192 (= G217), E219 (= E244), L282 (≠ V311), I324 (= I353), F337 (= F366), A338 (= A367), G339 (= G368), E354 (= E383), K358 (= K387)
5jxzA A low magnesium structure of the isochorismate synthase, entc (see paper)
38% identity, 93% coverage: 24:396/401 of query aligns to 4:372/373 of 5jxzA
- active site: K130 (= K145), E180 (= E200), A196 (= A216), E224 (= E244), H259 (= H279), A286 (= A310), F310 (≠ Y334), R330 (= R354), G346 (= G370), E359 (= E383), K363 (= K387)
- binding (5S,6S)-5-[(1-carboxyethenyl)oxy]-6-hydroxycyclohexa-1,3-diene-1-carboxylic acid: L195 (= L215), G197 (= G217), S198 (= S218), E224 (= E244), A286 (= A310), I329 (= I353), R330 (= R354), A343 (= A367), G344 (= G368), E359 (= E383), K363 (= K387)
- binding (3R,4R)-3-[(1-carboxyethenyl)oxy]-4-hydroxycyclohexa-1,5-diene-1-carboxylic acid: E180 (= E200), L195 (= L215), A196 (= A216), G197 (= G217), E224 (= E244), A286 (= A310), I329 (= I353), R330 (= R354), G344 (= G368), A345 (= A369), E359 (= E383), K363 (= K387)
- binding magnesium ion: E224 (= E244), E359 (= E383)
8w71A Structural basis of chorismate isomerization by arabidopsis isochorismate synthase ics1 (see paper)
34% identity, 68% coverage: 122:395/401 of query aligns to 227:493/494 of 8w71A
- binding (3R,4R)-3-[(1-carboxyethenyl)oxy]-4-hydroxycyclohexa-1,5-diene-1-carboxylic acid: R252 (≠ K145), E302 (= E200), A319 (≠ G217), T320 (≠ S218), H381 (= H279), A408 (= A310), Y432 (= Y334), I450 (= I353), R451 (= R354), A465 (= A367), G466 (= G368), K485 (= K387)
- binding magnesium ion: G468 (= G370), E478 (= E380)
8w6vA Structural basis of chorismate isomerization by arabidopsis isochorismate synthase ics1 (see paper)
34% identity, 68% coverage: 122:395/401 of query aligns to 227:493/494 of 8w6vA
P38051 Isochorismate synthase MenF; Isochorismate hydroxymutase; Isochorismate mutase; EC 5.4.4.2 from Escherichia coli (strain K12) (see 2 papers)
32% identity, 68% coverage: 122:392/401 of query aligns to 167:428/431 of P38051
- K190 (= K145) active site, Proton acceptor; mutation to A: Lack of activity.
- E240 (= E200) active site, Proton donor; mutation to Q: Lack of activity.
- L255 (= L215) mutation to A: Decrease in activity.
- E284 (= E244) binding Mg(2+)
- A344 (= A310) mutation to T: Lack of activity.
- R387 (= R354) mutation to A: Lack of activity.
- E416 (= E383) binding Mg(2+)
3bznA Crystal structure of open form of menaquinone-specific isochorismate synthase, menf (see paper)
32% identity, 68% coverage: 122:392/401 of query aligns to 167:428/430 of 3bznA
- active site: K190 (= K145), E240 (= E200), A256 (= A216), E284 (= E244), H318 (= H279), A344 (= A310), Y368 (= Y334), R387 (= R354), G403 (= G370), E416 (= E383), K420 (= K387)
- binding magnesium ion: E284 (= E244), E416 (= E383)
A0QX93 Anthranilate synthase component 1; AS; ASI; EC 4.1.3.27 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
31% identity, 67% coverage: 125:393/401 of query aligns to 249:513/524 of A0QX93
- K355 (≠ A233) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)
5cwaA Structure of anthranilate synthase component i (trpe) from mycobacterium tuberculosis with inhibitor bound (see paper)
31% identity, 67% coverage: 125:393/401 of query aligns to 228:492/505 of 5cwaA
- active site: Q248 (≠ K145), E301 (= E200), A317 (= A216), E345 (= E244), H382 (= H279), T409 (≠ A310), Y433 (= Y334), R453 (= R354), G469 (= G370), E482 (= E383), K486 (= K387)
- binding 3-{[(1Z)-1-carboxyprop-1-en-1-yl]oxy}-2-hydroxybenzoic acid: Y433 (= Y334), I452 (= I353), A466 (= A367), G467 (= G368), K486 (= K387)
7bvdA Anthranilate synthase component i (trpe)[mycolicibacterium smegmatis]
31% identity, 67% coverage: 125:393/401 of query aligns to 228:488/499 of 7bvdA
- active site: Q248 (≠ K145), E301 (= E200), A317 (= A216), E341 (= E244), H378 (= H279), T405 (≠ A310), Y429 (= Y334), R449 (= R354), G465 (= G370), E478 (= E383), K482 (= K387)
Sites not aligning to the query:
O94582 Probable anthranilate synthase component 1; Anthranilate synthase component I; EC 4.1.3.27 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
30% identity, 69% coverage: 125:400/401 of query aligns to 213:477/489 of O94582
- S390 (≠ C312) modified: Phosphoserine
- S392 (≠ T314) modified: Phosphoserine
Sites not aligning to the query:
- 488 modified: Phosphoserine
7pi1DDD Aminodeoxychorismate synthase component 1
27% identity, 66% coverage: 125:387/401 of query aligns to 193:445/459 of 7pi1DDD
Sites not aligning to the query:
P28820 Aminodeoxychorismate synthase component 1; ADC synthase; ADCS; 4-amino-4-deoxychorismate synthase component 1; EC 2.6.1.85 from Bacillus subtilis (strain 168) (see paper)
27% identity, 66% coverage: 125:387/401 of query aligns to 200:452/470 of P28820