SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_005457514.1 NCBI__GCF_000244975.1:WP_005457514.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

A6QDA0 N-(2-amino-2-carboxyethyl)-L-glutamate synthase; ACEGA synthase; Staphyloferrin B biosynthesis protein SbnA; EC 2.5.1.140 from Staphylococcus aureus (strain Newman) (see paper)
38% identity, 90% coverage: 17:310/328 of query aligns to 22:317/326 of A6QDA0

query
sites
A6QDA0

V

V

D

Q

L

L

E

H

S

Q

M

L

F

F

-

P

G

K

H

H

S

E

L

V

F

F

L

A

K

K

C

L

E

E

G

Y

F

M

N

N

F

P

A

G

G

G

S

S

I

M

K
|
K

L

D

K

R

A

P

A

A

I

K

E

Y

M

I

V

I

E

E

S

H

A

G

E

I

Q

K

H

H

G

G

L

L

V

I

T

T

P

E

D

N

T

T

I

H

L

L

V

I

E

E

S

S

S

T

S

S

G

G

N
|
N

L

L

G

G

V

I

A

A

L

L

A

A

I

M

I

I

A

A

A

K

S

I

K

K

G

G

Y

L

E

K

F

L

L

T

C

C

V

V

T

V

D

D

T

P

R

K

C

I

N

S

L

P

S

T

A

N

R

L

R

K

L

I

I

I

E

K

A

S

L

Y

G

G

S

A

Q

N

V

V

H

E

I

M

I

V

T

E

E

E

P

P

A

D

P

A

D

H

R

G

G

G

F

Y

L

L

G

M

A

T

R
|
R

L

I

D

A

Y

K

V

V

H

Q

S

E

L

L

C

L

A

A

S

T

D

I

P

D

R

D

Y

A

V

Y

W

W

L

I

N

N

Q

Q

Y
|
Y

S

A

N

N

P

E

S

L

N

N

W

W

K

Q

A

S

H

H

Y

Y

R

H

R

G

T

A

A

G

P

T

E

E

I

I

A

V

R

E

Q

T

F

I

P

K

N

Q

-

P

L

I

D

D

V

Y

L

F

F

V

I

A

G

P

T

V

G
x
S

T
|
T

G
|
G

T
x
S

L

I

M

M

G

G

C

M

A

S

R

R

Y

K

F

I

R

K

E

E

L

V

G

H

S

P

P

N

V

A

R

Q

I

I

V

V

A

A

V

V

D

D

S

A

V

K

G

G

S

S

V

V

T

I

F

F

G

G

G

D

E

K

P

P

G

I

R

N

R

R

M

E

I

L

P

P

G

G

L

I

G

G

T

A

S

S

V

R

R

V

P

P

R

E

L

I

L

L

D

N

E

R

S

S

Y

E

V

I

D

N

D

Q

V

V

V

I

I

H

V

V

D

D

E

D

V

Y

D

Q

T

S

I

A

R

L

A

G

C

C

H

R

R

K

L

L

A

I

R

D

-

Y

S

E

G

G

F

I

L

F

F

A

G

G

S
|
S

T

T

G

G

T

S

V

I

S

A

G

-

A

A

M

I

D

E

W

Q

L

L

A

I

R

T

H

S

G

I

S

E

P

E

G

G

M

A

T

T

S

I

V

V

A

T

I

I

A

L

P

P

D

D

L

R

G

G

E

D

R

R

Y

Y

L

L

D

D

T

L

L

V

Y

Y

Q

S

T

D

N

T

W

W

L

L

Q

E

G

K

L

M

K47 (= K41) modified: N6-(pyridoxal phosphate)lysine
N77 (= N71) binding pyridoxal 5'-phosphate
R132 (= R126) mutation to A: No detectable enzyme activity. Does not form pyridoxal 5'-phosphate-alpha-aminoacrylate reaction intermediate.
Y152 (= Y146) mutation to F: Very low enzyme activity. Does not form pyridoxal 5'-phosphate-alpha-aminoacrylate reaction intermediate; when associated with G-185.
STTGS 185:189 (≠ GTTGT 178:182) binding pyridoxal 5'-phosphate
S272 (= S264) binding pyridoxal 5'-phosphate

5d85A Staphyloferrin b precursor biosynthetic enzyme sbna bound to aminoacrylate intermediate (see paper)
38% identity, 90% coverage: 17:310/328 of query aligns to 14:309/313 of 5d85A

query
sites
5d85A

V

V

D

Q

L

L

E

H

S

Q

M

L

F

F

-

P

G

K

H

H

S

E

L

V

F

F

L

A

K

K

C

L

E

E

G

Y

F

M

N

N

F

P

A

G

G

G

S

S

I

M

K
|
K

L

D

K

R

A

P

A

A

I

K

E

Y

M

I

V

I

E

E

S

H

A

G

E

I

Q

K

H

H

G

G

L

L

V

I

T

T

P

E

D

N

T

T

I

H

L

L

V

I

E

E

S

S

S
x
T

S
|
S

G

G

N
|
N

L
|
L

G

G

V

I

A

A

L

L

A

A

I

M

I

I

A

A

A

K

S

I

K

K

G

G

Y

L

E

K

F

L

L

T

C

C

V

V

T

V

D

D

T

P

R
x
K

C

I

N

S

L

P

S

T

A

N

R

L

R

K

L

I

I

I

E

K

A

S

L

Y

G

G

S

A

Q

N

V

V

H

E

I

M

I

V

T

E

E

E

P

P

A

D

P

A

D

H

R

G

G
|
G

F
x
Y

L
|
L

G

M

A

T

R
|
R

L

I

D

A

Y

K

V

V

H

Q

S

E

L

L

C

L

A

A

S

T

D

I

P

D

R

D

Y

A

V

Y

W

W

L

I

N

N

Q
|
Q

Y

Y

S

A

N

N

P

E

S

L

N

N

W

W

K

Q

A

S

H

H

Y

Y

R

H

R

G

T

A

A

G

P

T

E

E

I

I

A

V

R

E

Q

T

F

I

P

K

N

Q

-

P

L

I

D

D

V

Y

L

F

F

V

I

A

G

P

T
x
V

G
x
S

T
|
T

G

G

T
x
S

L

I

M

M

G

G

C

M

A

S

R

R

Y

K

F

I

R

K

E

E

L

V

G

H

S

P

P

N

V

A

R

Q

I

I

V

V

A

A

V

V

D

D

S

A

V

K

G

G

S

S

V

V

T

I

F

F

G

G

G

D

E

K

P

P

G

I

R

N

R
|
R

M

E

I

L

P

P

G
|
G

L

I

G

G

T
x
A

S
|
S

V

R

R

V

P

P

R

E

L

I

L

L

D

N

E

R

S

S

Y

E

V

I

D

N

D

Q

V

V

V

I

I

H

V

V

D

D

E

D

V

Y

D

Q

T

S

I

A

R

L

A

G

C

C

H

R

R

K

L

L

A

I

R

D

-

Y

S

E

G

G

F

I

L

F

F

A

G

G

S
|
S

T

T

G

G

T

S

V

I

S

A

G

-

A

A

M

I

D

E

W

Q

L

L

A

I

R

T

H

S

G

I

S

E

P

E

G

G

M

A

T

T

S

I

V

V

A

T

I

I

A

L

P
|
P

D
|
D

L

R

G

G

E

D

R

R

Y

Y

L

L

D

D

T

L

L

V

Y

Y

Q

S

T

D

N

T

W

W

L

L

Q

E

G

K

L

M

active site: K39 (= K41), S264 (= S264)
binding citrate anion: S67 (= S69), K92 (≠ R94), G119 (= G121), Y120 (≠ F122), L121 (= L123), R124 (= R126), R216 (= R217), A223 (≠ T224), S224 (= S225)
binding 2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]acrylic acid: K39 (= K41), T66 (≠ S68), S67 (= S69), N69 (= N71), L70 (= L72), Q143 (= Q145), V176 (≠ T177), S177 (≠ G178), T178 (= T179), T179 (= T180), S181 (≠ T182), G220 (= G221), S264 (= S264), P290 (= P291), D291 (= D292)

5d84A Staphyloferrin b precursor biosynthetic enzyme sbna bound to plp (see paper)
38% identity, 90% coverage: 17:310/328 of query aligns to 16:311/318 of 5d84A

query
sites
5d84A

V

V

D

Q

L

L

E

H

S

Q

M

L

F

F

-

P

G

K

H

H

S

E

L

V

F

F

L

A

K

K

C

L

E

E

G

Y

F

M

N

N

F

P

A

G

G

G

S

S

I

M

K
|
K

L

D

K

R

A

P

A

A

I

K

E

Y

M

I

V

I

E

E

S

H

A

G

E

I

Q

K

H

H

G

G

L

L

V

I

T

T

P

E

D

N

T

T

I

H

L

L

V

I

E

E

S

S

S

T

S

S

G

G

N
|
N

L

L

G

G

V

I

A

A

L

L

A

A

I

M

I

I

A

A

A

K

S

I

K

K

G

G

Y

L

E

K

F

L

L

T

C

C

V

V

T

V

D

D

T

P

R

K

C

I

N

S

L

P

S

T

A

N

R

L

R

K

L

I

I

I

E

K

A

S

L

Y

G

G

S

A

Q

N

V

V

H

E

I

M

I

V

T

E

E

E

P

P

A

D

P

A

D

H

R

G

G

G

F

Y

L

L

G

M

A

T

R

R

L

I

D

A

Y

K

V

V

H

Q

S

E

L

L

C

L

A

A

S

T

D

I

P

D

R

D

Y

A

V

Y

W

W

L

I

N

N

Q

Q

Y

Y

S

A

N

N

P

E

S

L

N

N

W

W

K

Q

A

S

H

H

Y

Y

R

H

R

G

T

A

A

G

P

T

E

E

I

I

A

V

R

E

Q

T

F

I

P

K

N

Q

-

P

L

I

D

D

V

Y

L

F

F

V

I

A

G

P

T
x
V

G
x
S

T
|
T

G

G

T
x
S

L

I

M

M

G

G

C

M

A

S

R

R

Y

K

F

I

R

K

E

E

L

V

G

H

S

P

P

N

V

A

R

Q

I

I

V

V

A

A

V

V

D

D

S

A

V

K

G

G

S

S

V

V

T

I

F

F

G

G

G

D

E

K

P

P

G

I

R

N

R

R

M

E

I

L

P

P

G
|
G

L

I

G

G

T

A

S

S

V

R

R

V

P

P

R

E

L

I

L

L

D

N

E

R

S

S

Y

E

V

I

D

N

D

Q

V

V

V

I

I

H

V

V

D

D

E

D

V

Y

D

Q

T

S

I

A

R

L

A

G

C

C

H

R

R

K

L

L

A

I

R

D

-

Y

S

E

G

G

F

I

L

F

F

A

G

G

S
|
S

T

T

G

G

T

S

V

I

S

A

G

-

A

A

M

I

D

E

W

Q

L

L

A

I

R

T

H

S

G

I

S

E

P

E

G

G

M

A

T

T

S

I

V

V

A

T

I

I

A

L

P
|
P

D
|
D

L

R

G

G

E

D

R

R

Y

Y

L

L

D

D

T

L

L

V

Y

Y

Q

S

T

D

N

T

W

W

L

L

Q

E

G

K

L

M

active site: K41 (= K41), S266 (= S264)
binding pyridoxal-5'-phosphate: K41 (= K41), N71 (= N71), V178 (≠ T177), S179 (≠ G178), T180 (= T179), T181 (= T180), S183 (≠ T182), G222 (= G221), S266 (= S264), P292 (= P291), D293 (= D292)

5d86A Staphyloferrin b precursor biosynthetic enzyme sbna y152f variant (see paper)
37% identity, 90% coverage: 17:310/328 of query aligns to 15:310/318 of 5d86A

query
sites
5d86A

V

V

D

Q

L

L

E

H

S

Q

M

L

F

F

-

P

G

K

H

H

S

E

L

V

F

F

L

A

K

K

C

L

E

E

G

Y

F

M

N

N

F

P

A

G

G

G

S

S

I

M

K
|
K

L

D

K

R

A

P

A

A

I

K

E

Y

M

I

V

I

E

E

S

H

A

G

E

I

Q

K

H

H

G

G

L

L

V

I

T

T

P

E

D

N

T

T

I

H

L

L

V

I

E

E

S

S

S

T

S

S

G

G

N
|
N

L

L

G

G

V

I

A

A

L

L

A

A

I

M

I

I

A

A

A

K

S

I

K

K

G

G

Y

L

E

K

F

L

L

T

C

C

V

V

T

V

D

D

T

P

R

K

C

I

N

S

L

P

S

T

A

N

R

L

R

K

L

I

I

I

E

K

A

S

L

Y

G

G

S

A

Q

N

V

V

H

E

I

M

I

V

T

E

E

E

P

P

A

D

P

A

D

H

R

G

G

G

F

Y

L

L

G

M

A

T

R

R

L

I

D

A

Y

K

V

V

H

Q

S

E

L

L

C

L

A

A

S

T

D

I

P

D

R

D

Y

A

V

Y

W

W

L

I

N

N

Q

Q

Y

F

S

A

N

N

P

E

S

L

N

N

W

W

K

Q

A

S

H

H

Y

Y

R

H

R

G

T

A

A

G

P

T

E

E

I

I

A

V

R

E

Q

T

F

I

P

K

N

Q

-

P

L

I

D

D

V

Y

L

F

F

V

I

A

G

P

T
x
V

G
x
S

T
|
T

G

G

T
x
S

L

I

M

M

G

G

C

M

A

S

R

R

Y

K

F

I

R

K

E

E

L

V

G

H

S

P

P

N

V

A

R

Q

I

I

V

V

A

A

V

V

D

D

S

A

V

K

G

G

S

S

V

V

T

I

F

F

G

G

G

D

E

K

P

P

G

I

R

N

R

R

M

E

I

L

P

P

G
|
G

L

I

G

G

T

A

S
|
S

V
x
R

R

V

P

P

R

E

L

I

L

L

D

N

E

R

S

S

Y

E

V

I

D

N

D

Q

V

V

V

I

I

H

V

V

D

D

E

D

V

Y

D

Q

T

S

I

A

R

L

A

G

C

C

H

R

R

K

L

L

A

I

R

D

-

Y

S

E

G

G

F

I

L

F

F

A

G

G

S
|
S

T

T

G

G

T

S

V

I

S

A

G

-

A

A

M

I

D

E

W

Q

L

L

A

I

R

T

H

S

G

I

S

E

P

E

G

G

M

A

T

T

S

I

V

V

A

T

I

I

A

L

P
|
P

D
|
D

L

R

G

G

E

D

R

R

Y

Y

L

L

D

D

T

L

L

V

Y

Y

Q

S

T

D

N

T

W

W

L

L

Q

E

G

K

L

M

active site: K40 (= K41), S265 (= S264)
binding magnesium ion: S225 (= S225), R226 (≠ V226)
binding pyridoxal-5'-phosphate: K40 (= K41), N70 (= N71), V177 (≠ T177), S178 (≠ G178), T179 (= T179), T180 (= T180), S182 (≠ T182), G221 (= G221), S265 (= S264), P291 (= P291), D292 (= D292)

8if7A Crystal structure of cmnb (see paper)
42% identity, 88% coverage: 26:314/328 of query aligns to 37:325/342 of 8if7A

query
sites
8if7A

S

T

L

L

F

L

L

L

K

K

C

L

E

E

G

G

F

R

N

S

F

P

A

W

G

R

S

S

I

I

K
|
K

L

G

K

R

A

T

A

A

I

L

E

G

M

L

V

V

E

R

S

S

A

I

E

A

Q

P

H

R

G

-

L

M

V

A

T

S

P

R

D

D

T

V

I

T

L

V

V

V

E

E

S

S

S
x
T

S
|
S

G

G

N
|
N

L
|
L

G

G

V

V

A

A

L

L

A

S

I

A

I

I

A

C

A

R

S

D

K

L

G

G

Y

L

E

P

F

F

L

V

C

A

V

V

T

V

D

D

T

L

R

K

C

Q

N

S

L

P

S

V

A

I

R

Q

R

A

L

A

I

I

E

E

A

A

L

N

G

G

S

A

Q

R

V

L

H

E

I

V

T

R

E

T

P

P

A

A

P

A

D

A

R

T

G

T

F

H

L

L

G

L

A

D

R

R

L

L

D

D

Y

R

V

V

H

R

S

K

L

L

C

V

A

A

S

E

D

I

P

P

R

G

Y

A

V

V

W

W

L

P

N

N

Q

Q

Y

Y

S

E

N

N

P

D

S

A

N

N

W

R

K

H

A

V

H

H

Y

E

R

T

R

W

T

T

A

A

P

P

E

E

I

I

A

D

R

R

Q

Q

F

V

-

G

P

G

N

E

L

A

D

Q

V

A

L

V

F

F

I

V

G

A

T
x
V

G
x
S

T
|
T

T
x
G

G

G

T
|
T

L

L

M

A

G

G

C

L

A

A

R

A

Y

H

F

F

R

R

E

R

L

A

G

R

S

P

P

A

V

T

R

R

I

L

V

V

A

A

V

V

D

D

S

V

V

E

G

G

S

S

V

T

T

V

F

F

G

G

E

V

P

P

G

G

R

G

R

R

M

V

I

L

P

T

G
|
G

L

I

G

G

T

A

S

S

V

R

R

R

P

S

R

T

L

F

L

L

D

T

E

R

S

A

Y

E

V

C

D

D

V

L

V

V

I

Y

V

V

D

R

E

E

V

A

D

A

T

A

I

I

R

A

A

A

C

C

H

H

R

V

L

L

-

R

A

A

R

D

S

T

G

G

F

I

L

A

F

V

G

G

S
|
S

T

S

G

G

T

A

V

V

S

A

G

G

A

A

M

L

D

D

W

H

L

L

A

A

R

A

H

H

G

-

S

-

P

P

G

G

M

L

T

T

S

T

-

A

V

V

A

C

I

V

A

C

P
x
A

D
|
D

L

L

G

G

E

E

R

N

Y

Y

L

A

D

R

T

T

L

V

Y

Y

Q

D

T

P

N

D

W

W

L

L

Q

A

G

P

L

L

Y

R

G

L

E

T

D

D

binding 2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]acrylic acid: K52 (= K41), T78 (≠ S68), S79 (= S69), N81 (= N71), L82 (= L72), V188 (≠ T177), S189 (≠ G178), T190 (= T179), G191 (≠ T180), T193 (= T182), G232 (= G221), S276 (= S264), A302 (≠ P291), D303 (= D292)

P9WP55 O-acetylserine sulfhydrylase; OAS sulfhydrylase; OASS; Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine-specific cysteine synthase; Sulfide-dependent cysteine synthase; EC 2.5.1.47 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
36% identity, 84% coverage: 27:300/328 of query aligns to 30:302/310 of P9WP55

query
sites
P9WP55

L

I

F

V

L

A

K

K

C

L

E

E

G

F

F

F

N

N

F

P

A

A

G

N

S

S

I

V

K
|
K

L

D

K

R

A

I

A

G

I

V

E

A

M

M

V

L

E

Q

S

A

A

A

E

E

Q

Q

H

A

G

G

L

L

V

I

T

K

P

P

D

D

T

T

I

I

L

I

V

L

E

E

S

P

S

T

S

S

G

G

N
|
N

L

T

G

G

V

I

A

A

L

L

A

A

I

M

I

V

A

C

A

A

S

A

K

R

G

G

Y

Y

E

R

F

C

L

V

C

L

V

T

T

M

D

P

T

E

R

T

C

M

N

S

L

L

S

E

A

R

R

R

R

M

L

L

I

L

E

R

A

A

L

Y

G

G

S

A

Q

E

V

-

H

-

I

L

I

I

T

L

E

T

P

P

A

G

P

A

D

D

R

-

G

G

F

M

L

S

G

G

A

A

R

-

L

I

D

A

Y

K

V

A

H

E

S

E

L

L

C

A

A

K

S

T

D

D

P

Q

R

R

Y

Y

V

F

W

V

L

P

N

Q

Q

Q

Y

F

S

E

N

N

P

P

S

A

N

N

W

P

K

A

A

I

H

H

Y

R

R

V

R

T

T

T

A

A

P

E

E

E

I

V

A

W

R

R

Q

D

F

T

P

D

-

G

N

K

L

V

D

D

V

I

L

V

F

V

I

A

G

G

T

V

G
|
G

T
|
T

T
x
G

G
|
G

T
|
T

L

I

M

T

G

G

C

V

A

A

R

Q

Y

V

F

I

R

K

E

E

L

R

G

K

S

P

P

S

V

A

R

R

I

F

V

V

A

A

V

V

D

E

S

P

V

A

G

A

S

S

-

P

V

V

T

L

F

S

G

G

E

Q

P

K

G

G

R

P

R

H

M

P

I

I

P

Q

G

G

L

I

G

G

T

A

S

G

V

F

R

V

P

P

R

P

L

V

L

L

D

D

E

Q

S

D

Y

L

V

V

D

D

D

E

V

I

I

T

V

V

D

G

E

N

V

E

D

D

T

A

I

L

R

N

A

V

C

A

H

R

R

R

L

L

A

A

R

R

-

E

S

E

G

G

F

L

L

L

F

V

G

G

G

I

S
|
S

T

S

G

G

T

A

V

A

V

T

S

V

G

A

A

A

M

L

D

Q

W

V

L

A

A

R

R

R

H

P

G

E

S

N

P

A

G

G

M

K

T

L

S

I

V

V

A

V

I

V

A

L

P

P

D

D

L

F

G

G

E

E

R

R

Y

Y

L

L

D

S

T

T

K44 (= K41) modified: N6-(pyridoxal phosphate)lysine
N74 (= N71) binding pyridoxal 5'-phosphate
GTGGT 178:182 (≠ GTTGT 178:182) binding pyridoxal 5'-phosphate
S266 (= S264) binding pyridoxal 5'-phosphate

2q3dA 2.2 a resolution crystal structure of o-acetylserine sulfhydrylase (oass) from mycobacterium tuberculosis in complex with the reaction intermediate alpha-aminoacrylate (see paper)
36% identity, 84% coverage: 27:300/328 of query aligns to 30:302/306 of 2q3dA

query
sites
2q3dA

L

I

F

V

L

A

K

K

C

L

E

E

G

F

F

F

N

N

F

P

A

A

G

N

S

S

I

V

K
|
K

L

D

K

R

A

I

A

G

I

V

E

A

M

M

V

L

E

Q

S

A

A

A

E

E

Q

Q

H

A

G

G

L

L

V

I

T

K

P

P

D

D

T

T

I

I

L

I

V

L

E

E

S

P

S
x
T

S
|
S

G

G

N
|
N

L
x
T

G

G

V

I

A

A

L

L

A

A

I

M

I

V

A

C

A

A

S

A

K

R

G

G

Y

Y

E

R

F

C

L

V

C

L

V

T

T

M

D

P

T

E

R

T

C

M

N

S

L

L

S

E

A

R

R

R

R

M

L

L

I

L

E

R

A

A

L

Y

G

G

S

A

Q

E

V

-

H

-

I

L

I

I

T

L

E

T

P

P

A

G

P

A

D

D

R

-

G

G

F

M

L

S

G

G

A

A

R

-

L

I

D

A

Y

K

V

A

H

E

S

E

L

L

C

A

A

K

S

T

D

D

P

Q

R

R

Y

Y

V

F

W

V

L

P

N

Q

Q
|
Q

Y

F

S

E

N

N

P

P

S

A

N

N

W

P

K

A

A

I

H

H

Y

R

R

V

R

T

T

T

A

A

P

E

E

E

I

V

A

W

R

R

Q

D

F

T

P

D

-

G

N

K

L

V

D

D

V

I

L

V

F

V

I

A

G

G

T
x
V

G
|
G

T
|
T

T
x
G

G

G

T
|
T

L

I

M

T

G

G

C

V

A

A

R

Q

Y

V

F

I

R

K

E

E

L

R

G

K

S

P

P

S

V

A

R

R

I

F

V

V

A

A

V

V

D

E

S

P

V

A

G

A

S

S

-

P

V

V

T

L

F

S

G

G

E

Q

P

K

G

G

R

P

R

H

M

P

I

I

P

Q

G
|
G

L
x
I

G

G

T

A

S

G

V

F

R

V

P

P

R

P

L

V

L

L

D

D

E

Q

S

D

Y

L

V

V

D

D

D

E

V

I

I

T

V

V

D

G

E

N

V

E

D

D

T

A

I

L

R

N

A

V

C

A

H

R

R

R

L

L

A

A

R

R

-

E

S

E

G

G

F

L

L

L

F

V

G

G

G

I

S
|
S

T

S

G

G

T

A

V

A

V

T

S

V

G

A

A

A

M

L

D

Q

W

V

L

A

A

R

R

R

H

P

G

E

S

N

P

A

G

G

M

K

T

L

S

I

V

V

A

V

I

V

A

L

P
|
P

D
|
D

L

F

G

G

E

E

R

R

Y

Y

L

L

D

S

T

T

active site: K44 (= K41), S266 (= S264), P293 (= P291)
binding 2-[(3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl)-amino]-propionic acid: K44 (= K41), T71 (≠ S68), S72 (= S69), N74 (= N71), T75 (≠ L72), Q144 (= Q145), V177 (≠ T177), G178 (= G178), T179 (= T179), G180 (≠ T180), T182 (= T182), G222 (= G221), I223 (≠ L222), S266 (= S264), P293 (= P291), D294 (= D292)

3zeiA Structure of the mycobacterium tuberculosis o-acetylserine sulfhydrylase (oass) cysk1 in complex with a small molecule inhibitor (see paper)
36% identity, 83% coverage: 27:298/328 of query aligns to 30:300/300 of 3zeiA

query
sites
3zeiA

L

I

F

V

L

A

K

K

C

L

E

E

G

F

F

F

N

N

F

P

A

A

G

N

S

S

I

V

K
|
K

L

D

K

R

A

I

A

G

I

V

E

A

M

M

V

L

E

Q

S

A

A

A

E

E

Q

Q

H

A

G

G

L

L

V

I

T

K

P

P

D

D

T

T

I

I

L

I

V

L

E

E

S

P

S
x
T

S
|
S

G

G

N
|
N

L

T

G

G

V

I

A

A

L

L

A

A

I

M

I

V

A

C

A

A

S

A

K

R

G

G

Y

Y

E

R

F

C

L

V

C

L

V

T

T

M

D

P

T

E

R

T

C

M

N

S

L

L

S

E

A

R

R

R

R

M

L

L

I

L

E

R

A

A

L

Y

G

G

S

A

Q

E

V

-

H

-

I

L

I

I

T

L

E

T

P

P

A

G

P

A

D

D

R

-

G

G

F

M

L

S

G

G

A

A

R

-

L
x
I

D

A

Y

K

V

A

H

E

S

E

L

L

C

A

A

K

S

T

D

D

P

Q

R

R

Y

Y

V

F

W

V

L

P

N

Q

Q
|
Q

Y
x
F

S

E

N

N

P

P

S

A

N

N

W

P

K

A

A

I

H

H

Y

R

R

V

R

T

T

T

A

A

P

E

E

E

I

V

A

W

R

R

Q

D

F

T

P

D

-

G

N

K

L

V

D

D

V

I

L

V

F

V

I

A

G

G

T
x
V

G
|
G

T
|
T

T
x
G

G

G

T
|
T

L

I

M

T

G

G

C

V

A

A

R

Q

Y

V

F

I

R

K

E

E

L

R

G

K

S

P

P

S

V

A

R

R

I

F

V

V

A

A

V

V

D

E

S

P

V

A

G

A

S

S

-

P

V

V

T

L

F

S

G

G

E

Q

P
x
K

G

G

R

P

R

H

M

P

I

I

P

Q

G
|
G

L

I

G

G

T
x
A

S

G

V
x
F

R

V

P

P

R

P

L

V

L

L

D

D

E

Q

S

D

Y

L

V

V

D

D

D

E

V

I

I

T

V

V

D

G

E

N

V

E

D

D

T

A

I

L

R

N

A

V

C

A

H

R

R

R

L

L

A

A

R

R

-

E

S

E

G

G

F

L

L

L

F

V

G

G

G

I

S
|
S

T

S

G

G

T

A

V

A

V

T

S

V

G

A

A

A

M

L

D

Q

W

V

L

A

A

R

R

R

H

P

G

E

S

N

P

A

G

G

M

K

T

L

S

I

V

V

A

V

I

V

A

L

P
|
P

D
|
D

L

F

G

G

E

E

R

R

Y

Y

L

L

active site: K44 (= K41), S266 (= S264), P293 (= P291)
binding 3-[(Z)-[(5Z)-5-[[2-(2-hydroxy-2-oxoethyloxy)phenyl]methylidene]-3-methyl-4-oxidanylidene-1,3-thiazolidin-2-ylidene]amino]benzoic acid: T71 (≠ S68), S72 (= S69), I126 (≠ L127), Q144 (= Q145), F145 (≠ Y146), K215 (≠ P214), G222 (= G221), A225 (≠ T224), F227 (≠ V226)
binding pyridoxal-5'-phosphate: K44 (= K41), N74 (= N71), V177 (≠ T177), G178 (= G178), T179 (= T179), G180 (≠ T180), T182 (= T182), G222 (= G221), S266 (= S264), P293 (= P291), D294 (= D292)

2q3cA 2.1 a resolution crystal structure of o-acetylserine sulfhydrylase (oass) holoenzyme from mycobacterium tuberculosis in complex with the inhibitory peptide dfsi (see paper)
36% identity, 83% coverage: 27:298/328 of query aligns to 30:300/300 of 2q3cA

query
sites
2q3cA

L

I

F

V

L

A

K

K

C

L

E

E

G

F

F

F

N

N

F

P

A

A

G

N

S

S

I

V

K
|
K

L

D

K

R

A

I

A

G

I

V

E

A

M

M

V

L

E

Q

S

A

A

A

E

E

Q

Q

H

A

G

G

L

L

V

I

T

K

P

P

D

D

T

T

I

I

L

I

V

L

E

E

S

P

S
x
T

S
|
S

G
|
G

N

N

L
x
T

G

G

V

I

A

A

L

L

A

A

I

M

I

V

A

C

A

A

S

A

K

R

G

G

Y

Y

E

R

F

C

L

V

C

L

V

T

T

M

D

P

T

E

R

T

C

M

N

S

L

L

S

E

A

R

R

R

R

M

L

L

I

L

E

R

A

A

L

Y

G

G

S

A

Q

E

V

-

H

-

I

L

I

I

T

L

E

T

P

P

A

G

P

A

D

D

R

-

G

G

F
x
M

L

S

G

G

A

A

R

-

L

I

D

A

Y

K

V

A

H

E

S

E

L

L

C

A

A

K

S

T

D

D

P

Q

R

R

Y

Y

V

F

W

V

L

P

N

Q

Q
|
Q

Y

F

S

E

N

N

P

P

S

A

N

N

W

P

K

A

A

I

H

H

Y

R

R

V

R

T

T

T

A

A

P

E

E

E

I

V

A

W

R

R

Q

D

F

T

P

D

-

G

N

K

L

V

D

D

V

I

L

V

F

V

I

A

G

G

T

V

G

G

T

T

T

G

G

G

T

T

L

I

M

T

G

G

C

V

A

A

R

Q

Y

V

F

I

R

K

E

E

L

R

G

K

S

P

P

S

V

A

R

R

I

F

V

V

A

A

V

V

D

E

S

P

V

A

G

A

S

S

-

P

V

V

T

L

F

S

G

G

E

Q

P
x
K

G

G

R

P

R

H

M

P

I

I

P

Q

G
|
G

L

I

G

G

T
x
A

S

G

V

F

R

V

P

P

R

P

L

V

L

L

D

D

E

Q

S

D

Y

L

V

V

D

D

D

E

V

I

I

T

V

V

D

G

E

N

V

E

D

D

T

A

I

L

R

N

A

V

C

A

H

R

R

R

L

L

A

A

R

R

-

E

S

E

G

G

F

L

L

L

F

V

G

G

G

I

S
|
S

T

S

G

G

T

A

V

A

V

T

S

V

G

A

A

A

M

L

D

Q

W

V

L

A

A

R

R

R

H

P

G

E

S

N

P

A

G

G

M

K

T

L

S

I

V

V

A

V

I

V

A

L

P
|
P

D

D

L

F

G

G

E

E

R

R

Y

Y

L

L

active site: K44 (= K41), S266 (= S264), P293 (= P291)
binding : T71 (≠ S68), S72 (= S69), G73 (= G70), T75 (≠ L72), M122 (≠ F122), Q144 (= Q145), K215 (≠ P214), G222 (= G221), A225 (≠ T224)

2bhtA Crystal structure of o-acetylserine sulfhydrylase b (see paper)
35% identity, 88% coverage: 24:313/328 of query aligns to 24:294/294 of 2bhtA

query
sites
2bhtA

G

G

H

S

S

E

L

V

F

W

L

L

K

K

C

L

E

E

G

G

F

N

N

N

F

P

A

A

G

G

S

S

I

V

K
|
K

L

D

K

R

A

A

I

L

E

S

M

M

V

I

E

V

S

E

A

A

E

E

Q

K

H

R

G

G

L

R

V

I

T

K

P

P

D

G

T

D

I

V

L

L

V

I

E

E

S

A

S

T

S
|
S

G

G

N
|
N

L

T

G

G

V

I

A

A

L

L

A

A

I

M

I

I

A

A

S

L

K

K

G

G

Y

Y

E

R

F

M

L

K

C

L

V

L

T

M

D

P

T

D

R

N

C

M

N

S

L

Q

S

E

A

R

R

R

R

A

L

A

I

M

E

R

A

A

L

Y

G

G

S

A

Q

E

V

L

H

I

I

L

I

V

T

T

E

K

P

-

A

-

P

-

D

E

R

Q

G

G

F

M

L

E

G

G

A

A

R

R

L

-

D

D

Y

L

V

A

H

L

S

E

L

M

C

-

A

A

S

N

D

R

P

G

R

E

Y

G

V

K

W

L

L

L

N

D

Q

Q

Y

F

S

N

N

N

P

P

S

D

N

N

W

P

K

K

A

A

H

H

Y

Y

R

T

T

T

A

G

P

P

E

E

I

I

A

W

R

Q

Q

Q

F

T

P

G

N

G

L

R

D

I

V

T

L

H

F

F

I

V

G

S

T

S

-
x
M

G
|
G

T
|
T

G

G

T
|
T

L

I

M

T

G

G

C

V

A

S

R

E

Y

F

F

M

R

R

E

E

L

Q

G

S

S

K

P

P

V

V

R

T

I

I

V

V

A

G

V

L

D
x
Q

S

P

V

E

G

E

S
x
G

V

S

T

S

F

I

G

-

E

-

P

P

G
|
G

R

I

R

R

M

R

I

W

P

P

G

-

L

-

G

-

T

T

S

E

V

Y

R

L

P

P

R

G

L

I

L

F

D

N

E

A

S

S

Y

L

V

V

D

D

D

E

V

V

V

L

I

D

V

I

D

H

E

Q

V

R

D

D

T

A

I

E

R

N

A

T

C

M

H

R

R

E

L

L

A

A

-

V

R

R

S

E

G

G

F

I

L

F

F

C

G

G

G

V

S
|
S

T

S

G

G

T

G

V

A

V

V

S

A

G

G

A

A

M

L

D

R

W

-

L

-

A

V

R

A

H

K

G

A

S

N

P

P

G

D

M

A

T

V

S

V

V

V

A

A

I

I

A

I

P
x
C

D

D

L

R

G

G

E

D

R

R

Y

Y

L

L

D

S

T

T

L

-

Y

-

Q

-

T

-

N

-

W

-

L

-

Q

-

G

G

L

V

Y

F

G

G

E

E

active site: K41 (= K41), S69 (= S69), Q199 (≠ D203), G203 (≠ S207), S255 (= S264), C280 (≠ P291)
binding pyridoxal-5'-phosphate: K41 (= K41), N71 (= N71), M173 (vs. gap), G174 (= G178), T175 (= T179), T176 (= T180), T178 (= T182), G208 (= G215), S255 (= S264), C280 (≠ P291)

P16703 Cysteine synthase B; CSase B; O-acetylserine (thiol)-lyase B; OAS-TL B; O-acetylserine sulfhydrylase B; EC 2.5.1.47 from Escherichia coli (strain K12) (see paper)
35% identity, 89% coverage: 24:314/328 of query aligns to 24:295/303 of P16703

query
sites
P16703

G

G

H

S

S

E

L

V

F

W

L

L

K

K

C

L

E

E

G

G

F

N

N

N

F

P

A

A

G

G

S

S

I

V

K

K

L

D

K

R

A

A

I

L

E

S

M

M

V

I

E

V

S

E

A

A

E

E

Q

K

H

R

G

G

L

E

V

I

T

K

P

P

D

G

T

D

I

V

L

L

V

I

E

E

S

A

S

T

S

S

G

G

N
|
N

L

T

G

G

V

I

A

A

L

L

A

A

I

M

I

I

A

A

S

L

K

K

G

G

Y

Y

E

R

F

M

L

K

C

L

V

L

T

M

D

P

T

D

R

N

C

M

N

S

L

Q

S

E

A

R

R

R

R

A

L

A

I

M

E

R

A

A

L

Y

G

G

S

A

Q

E

V

L

H

I

I

L

I

V

T

T

E

K

P

-

A

-

P

-

D

E

R

Q

G

G

F

M

L

E

G

G

A

A

R

R

L

-

D

D

Y

L

V

A

H

L

S

E

L

M

C

-

A

A

S

N

D

R

P

G

R

E

Y

G

V

K

W

L

L

L

N

D

Q

Q

Y

F

S

N

N

N

P

P

S

D

N

N

W

P

K

Y

A

A

H

H

Y

Y

R

T

T

T

A

G

P

P

E

E

I

I

A

W

R

Q

Q

Q

F

T

P

G

N

G

L

R

D

I

V

T

L

H

F

F

I

V

G

S

T

S

-

M

G

G

T

T

G

G

T

T

L

I

M

T

G

G

C

V

A

S

R

R

Y

F

F

M

R

R

E

E

L

Q

G

S

S

K

P

P

V

V

R

T

I

I

V

V

A

G

V

L

D

Q

S

P

V

E

G

E

S

G

V

S

T

S

F

I

G

-

E

-

P

P

G

G

R

I

R

R

M

R

I

W

P

P

G

-

L

-

G

-

T

T

S

E

V

Y

R

L

P

P

R

G

L

I

L

F

D

N

E

A

S

S

Y

L

V

V

D

D

D

E

V

V

V

L

I

D

V

I

D

H

E

Q

V

R

D

D

T

A

I

E

R

N

A

T

C

M

H

R

R

E

L

L

A

A

-

V

R

R

S

E

G

G

F

I

L

F

F

C

G

G

G

V

S
|
S

T

S

G

G

T

G

V

A

V

V

S

A

G

G

A

A

M

L

D

R

W

-

L

-

A

V

R

A

H

K

G

A

S

N

P

P

G

D

M

A

T

V

S

V

V

V

A

A

I

I

A

I

P

C

D

D

L

R

G

G

E

D

R

R

Y

Y

L

L

D

S

T

T

L

-

Y

-

Q

-

T

-

N

-

W

-

L

-

Q

-

G

G

L

V

Y

F

G

G

E

E

D

E

N71 (= N71) binding pyridoxal 5'-phosphate
S255 (= S264) binding pyridoxal 5'-phosphate

Q79FV4 S-sulfocysteine synthase; O-phospho-L-serine-dependent S-sulfocysteine synthase; OPS-dependent S-sulfocysteine synthase; O-phosphoserine sulfhydrylase; EC 2.8.5.1; EC 2.5.1.65 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
36% identity, 84% coverage: 27:303/328 of query aligns to 52:326/372 of Q79FV4

query
sites
Q79FV4

L

F

F

W

L

A

K

K

C

L

E

E

G

G

F

F

N

N

F

-

A

P

G

G

S

G

I

M

K

K

L

D

K

R

A

P

A

A

I

L

E

Y

M

M

V

V

E

E

S

C

A

A

E

R

Q

A

H

R

G

G

L

D

V

I

T

A

P

P

D

G

T

A

I

A

L

I

V

V

E

E

S

S

S

T

S

G

G

G

N

T

L

L

G

G

V

L

A

G

L

L

A

A

I

L

I

A

A

G

A

K

S

V

K

Y

G

R

Y

H

E

P

F

V

L

T

C

L

V

V

T

T

D

D

T

P

R

G

C

L

N

E

L

P

S

I

A

I

R

A

R

R

L

M

I

L

E

T

A

A

L

Y

G

G

S

A

Q

G

V

V

H

D

I

M

I

V

T

T

E

Q

P

P

A

H

P

P

D

V

R

G

G

G

F

W

L

Q

G

Q

A

A

R

R

L

K

D

D

Y

R

V

V

H

A

S

Q

L

L

C

M

A

A

S

E

D

Y

P

P

R

G

Y

A

V

W

W

N

L

P

N

N

Q

Q

Y

Y

S

G

N

N

P

P

S

D

N

N

W

V

K

G

A

A

H

-

Y

Y

R

R

R

S

T

L

A

A

P

L

E

E

I

L

A

V

R

A

Q

Q

F

L

P

G

N

R

L

I

D

D

V

V

L

L

F

V

I

C

G

S

T

V

G

G

T

T

T

G

G

G

T

H

L

S

M

A

G

G

C

V

A

A

R

R

Y

V

F

L

R

R

E

E

L

F

G

N

S

P

P

D

V

M

R

R

I

L

V

I

A

G

V

V

D

D

S

T

V

I

G

G

S

S

V

T

T

I

F

F

G

G

G

Q

E

P

P

A

G

S

R

N

R

R

M

L

I

M

P
x
R

G

G

L

L

G

G

T

S

S

S

V

I

R

Y

P

P

R

R

L

N

L

V

D

D

E

Y

S

R

Y

A

V

F

D

D

D

E

V

V

V

H

I

W

V

V

D

A

E

P

V

P

D

E

T

A

I

V

R

W

A

A

C

C

H

R

R

S

L

L

A

A

R

A

S

T

G

H

F

Y

L

A

F

S

G

G

S

W

T

S

G

V

T

G

V

A

V

V

S

A

G

L

A

V

M

A

D

G

W

W

L

A

A

A

R

R

H

N

G

L

S

P

P

A

G

D

M

T

T

T

S

I

V

A

A

A

I

V

A

F

P

P

D

D

L

G

G

P

E

Q

R

R

Y

Y

L

F

D

D

T

T

L

I

Y

Y

Q

N

R243 (≠ P220) mutation to A: 10-fold decrease in the specificity constant for thiosulfate compared to wild-type enzyme but little change in the specificity constant for the substrate OPS.

P47999 Cysteine synthase, chloroplastic/chromoplastic; At.OAS.7-4; Beta-substituted Ala synthase 2;1; ARAth-Bsas2;1; CSase B; AtCS-B; CS-B; O-acetylserine (thiol)-lyase; O-acetylserine sulfhydrylase; OAS-TL B; cpACS1; EC 2.5.1.47 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
35% identity, 85% coverage: 26:304/328 of query aligns to 101:380/392 of P47999

query
sites
P47999

S

S

L

V

F

A

L

A

K

K

C

L

E

E

G

I

F

M

N

E

F

P

A

C

G

C

S

S

I

V

K

K

L

D

K

R

A

I

A

G

I

Y

E

S

M

M

V

I

E

T

S

D

A

A

E

E

Q

E

H

K

G

G

L

L

V

I

T

T

P

P

-

G

D

K

T

S

I

V

L

L

V

V

E

E

S

S

S

T

S

S

G

G

N

N

L

T

G

G

V

I

A

G

L

L

A

A

I

F

I

I

A

A

S

S

K

K

G

G

Y

Y

E

K

F

L

L

I

C

L

V

T

T

M

D

P

T

A

R

S

C

M

N

S

L

L

S

E

A

R

R

R

R

V

L

L

I

L

E

R

A

A

L

F

G

G

S

A

Q

E

V

L

H

-

I

V

I

L

T

T

E

E

P

P

A

A

P

-

D

-

R

K

G

G

F

M

L

T

G

G

A

A

R

-

L

I

D

Q

Y

K

V

A

H

E

S

E

L

I

C

L

A

K

S

K

D

T

P

P

R

N

Y

S

V

Y

W

M

L

L

N

Q

Q

Q

Y

F

S

D

N

N

P

P

S

A

N

N

W

P

K

K

A

I

H

H

Y

Y

R

E

R

T

T

T

A

G

P

P

E

E

I

I

A

W

R

E

Q

D

F

T

P

R

-

G

N

K

L

I

D

D

V

I

L

L

F

V

I

A

G

G

T

I

G

G

T

T

T

G

G

G

T

T

L

I

M

T

G

G

C

V

A

G

R

R

Y

F

F

I

R

K

E

E

L

R

G

K

S

P

P

E

V

L

R

K

I

V

V

I

A

G

V

V

D

E

S

P

V

T

G

E

S

S

V

A

T

I

F

L

-

S

G

G

E

K

P

P

G

G

R

P

R

H

M

K

I

I

P

Q

G

G

L

I

G

G

T

A

S

G

V

F

R

V

P

P

R

K

L

N

L

L

D

D

E

L

S

A

Y

I

V

V

D

D

D

E

V

Y

V

I

I

A

V

I

D

S

E

S

V

E

D

E

T

A

I

I

R

E

A

T

C

S

H

K

R

Q

L

L

A

A

-

L

R

Q

S

E

G

G

F

L

L

L

F

V

G

G

G

I

S

S

T

S

G

G

T

A

V

A

S

A

G

A

A

A

M

I

D

Q

W

V

L

A

A

K

R

R

H

P

G

E

S

N

P

A

G

G

M

K

T

L

S

I

V

A

A

V

I

V

A

F

P

P

D

S

L

F

G

G

E

E

R

R

Y

Y

L

L

D

S

T

T

-

Q

L

L

Y

F

Q

Q

T

S

Sites not aligning to the query:

61 modified: N-acetylalanine

5xenA Crystal structure of a hydrogen sulfide-producing enzyme (fn1220) from fusobacterium nucleatum in complex with l-serine-plp schiff base
33% identity, 84% coverage: 23:298/328 of query aligns to 24:300/300 of 5xenA

query
sites
5xenA

F

F

G

G

H

N

S

E

L

I

F

Y

L

V

K

K

C

L

E

E

G

G

F

S

N

N

F

P

A

G

G

R

S

S

I

T

K
|
K

L

D

K

R

A

I

A

A

I

L

E

K

M

M

V

I

E

E

S

E

A

A

E

E

Q

K

H

E

G

G

L

L

V

I

T

D

P

K

D

D

T

T

I

V

L

I

V

I

E

E

S

A

S
x
T

S
|
S

G

G

N
|
N

L
x
T

G

G

V

I

A

G

L

L

A

A

I

M

I

I

A

C

A

A

S

V

K

K

G

N

Y

Y

E

K

F

L

L

K

C

I

V

V

T

M

D

P

T

D

R

T

C

M

N

S

L

I

S

E

A

R

R

I

R

Q

L

L

I

M

E

R

A

A

L

Y

G

G

S

T

Q

E

V

V

H

-

I

I

I

L

T

T

E

D

P

-

A

-

P

-

D

-

R

-

G

G

F

S

L

L

G

G

A

M

R

K

-

A

-

C

L

L

D

E

Y

K

V

L

H

E

S

E

L

L

C

K

A

K

S

N

D

E

P

K

R

K

Y

Y

V

F

W

V

L

P

N

N

Q
|
Q

Y

F

S

T

N

N

P

V

S

N

N

N

W

P

K

K

A

A

H

H

Y

Y

R

E

R

T

T

T

A

A

P

E

E

E

I

I

A

L

R

K

Q

D

F

L

P

N

N

N

-

K

L

V

D

D

V

V

L

F

F

I

I

C

G

G

T
|
T

G
|
G

T
|
T

T
x
G

G

G

T
x
S

L

F

M

S

G

G

C

T

A

A

R

K

Y

K

F

L

R

K

E

E

L

K

G

L

S

P

P

N

V

I

R

K

I

T

V

F

A

P

V

V

D

E

S

P

V

A

G

S

S

S

-

P

V

L

T

L

F

S

G

K

G

G

E

Y

P

I

G

G

R

P

R

H

M

K

I

I

P

Q

G
|
G

L

M

G

G

T

M

S

S

V

I

R

G

-

G

-

I

P

P

R

A

L

V

L

Y

D

D

E

G

S

S

Y

L

V

A

D

D

V

I

V

L

I

V

V

C

D

E

E

D

V

D

D

D

T

A

I

F

R

E

A

M

C

M

H

R

R

E

L

L

A

S

-

F

R

K

S

E

G

G

F

I

L

L

F

G

G

G

G

I

S
|
S

T

T

G

G

T

A

V

T

V

F

S

K

G

A

A

A

M

L

D

D

W

Y

L

S

A

K

R

E

H

N

G

A

S

D

P

K

G

G

M

L

T

K

S

I

V

V

A

V

I

L

A

S

P
x
T

D
|
D

L

S

G

G

E

E

R

K

Y

Y

L

L

binding pyridoxal-5'-phosphate: K42 (= K41), K42 (= K41), N72 (= N71), N72 (= N71), T175 (= T177), T175 (= T177), G176 (= G178), G176 (= G178), T177 (= T179), T177 (= T179), G178 (≠ T180), G178 (≠ T180), S180 (≠ T182), S180 (≠ T182), G220 (= G221), G220 (= G221), S266 (= S264), S266 (= S264), T293 (≠ P291), T293 (≠ P291), D294 (= D292), D294 (= D292)
binding serine: K42 (= K41), T69 (≠ S68), S70 (= S69), N72 (= N71), T73 (≠ L72), Q142 (= Q145)

5xemA Crystal structure of a hydrogen sulfide-producing enzyme (fn1220) from fusobacterium nucleatum in complex with l-lanthionine-plp schiff base
33% identity, 84% coverage: 23:298/328 of query aligns to 24:300/302 of 5xemA

query
sites
5xemA

F

F

G

G

H

N

S

E

L

I

F

Y

L

V

K

K

C

L

E

E

G

G

F

S

N

N

F

P

A

G

G

R

S

S

I

T

K
|
K

L

D

K

R

A

I

A

A

I

L

E

K

M

M

V

I

E

E

S

E

A

A

E

E

Q

K

H

E

G

G

L

L

V

I

T

D

P

K

D

D

T

T

I

V

L

I

V

I

E

E

S

A

S
x
T

S
|
S

G

G

N
|
N

L
x
T

G

G

V

I

A

G

L

L

A

A

I

M

I

I

A

C

A

A

S

V

K

K

G

N

Y

Y

E

K

F

L

L

K

C

I

V

V

T

M

D

P

T

D

R

T

C

M

N

S

L

I

S

E

A

R

R

I

R

Q

L

L

I

M

E

R

A

A

L

Y

G

G

S

T

Q

E

V

V

H

-

I

I

I

L

T

T

E

D

P

-

A

-

P

-

D

-

R

-

G

G

F

S

L

L

G

G

A
x
M

R

K

-

A

-

C

L

L

D

E

Y

K

V

L

H

E

S

E

L

L

C

K

A

K

S

N

D

E

P

K

R

K

Y

Y

V

F

W

V

L

P

N

N

Q
|
Q

Y

F

S

T

N

N

P

V

S

N

N

N

W

P

K

K

A

A

H

H

Y

Y

R

E

R

T

T

T

A

A

P

E

E

E

I

I

A

L

R

K

Q

D

F

L

P

N

N

N

-

K

L

V

D

D

V

V

L

F

F

I

I

C

G

G

T
|
T

G
|
G

T
|
T

T
x
G

G

G

T
x
S

L

F

M

S

G

G

C

T

A

A

R

K

Y

K

F

L

R

K

E

E

L

K

G

L

S

P

P

N

V

I

R

K

I

T

V

F

A

P

V

V

D

E

S

P

V

A

G

S

S

S

-

P

V

L

T

L

F

S

G

K

G

G

E

Y

P

I

G

G

R

P

R

H

M

K

I

I

P

Q

G
|
G

L
x
M

G
|
G

T

M

S
|
S

V

I

R

G

-

G

-

I

P

P

R

A

L

V

L

Y

D

D

E

G

S

S

Y

L

V

A

D

D

V

I

V

L

I

V

V

C

D

E

E

D

V

D

D

D

T

A

I

F

R

E

A

M

C

M

H

R

R

E

L

L

A

S

-

F

R

K

S

E

G

G

F

I

L

L

F

G

G

G

G

I

S
|
S

T

T

G

G

T

A

V

T

V

F

S

K

G

A

A

A

M

L

D

D

W

Y

L

S

A

K

R

E

H

N

G

A

S

D

P

K

G

G

M

L

T

K

S

I

V

V

A

V

I

L

A

S

P
x
T

D
|
D

L

S

G

G

E

E

R

K

Y

Y

L
|
L

binding (2R)-2-azanyl-3-[(2R)-2-azanyl-3-oxidanyl-3-oxidanylidene-propyl]sulfanyl-propanoic acid: K42 (= K41), T69 (≠ S68), S70 (= S69), N72 (= N71), T73 (≠ L72), M120 (≠ A125), Q142 (= Q145), G176 (= G178), T177 (= T179), G220 (= G221), M221 (≠ L222), G222 (= G223), S224 (= S225)
binding calcium ion: L300 (= L298)
binding pyridoxal-5'-phosphate: K42 (= K41), N72 (= N71), T175 (= T177), G176 (= G178), T177 (= T179), G178 (≠ T180), S180 (≠ T182), G220 (= G221), S266 (= S264), T293 (≠ P291), D294 (= D292)

Sites not aligning to the query:

binding calcium ion: 301, 302

Q93244 Cysteine synthase 1; O-acetylserine (thiol)-lyase 1; OAS-TL; EC 2.5.1.47 from Caenorhabditis elegans (see 2 papers)
36% identity, 91% coverage: 6:305/328 of query aligns to 12:312/341 of Q93244

query
sites
Q93244

A

A

P

A

L

L

E

I

F

G

N

N

E

T

E

P

Q

M

L

V

Y

Y

V

I

D

N

-

K

L

L

E

T

S

K

M

G

F

L

G

P

H

G

S

T

L

V

F

A

L

V

K

K

C

I

E

E

G

Y

F

M

N

N

F

P

A

A

G

G

S

S

I

V

K

K

L

D

K

R

A

I

A

G

I

A

E

A

M

M

V

L

E

A

S

A

A

A

E

E

Q

K

H

D

G

G

L

T

V

V

T

I

P

P

D

G

-

V

T

T

I

T

L

L

V

I

E

E

S
x
P

S

T

S

S

G

G

N

N

L

T

G

G

V

I

A

A

L

L

A

A

I

F

I

V

A
|
A

A

A

S

A

K

K

G

G

Y

Y

E

R

F

-

L

-

C

C

V

I

T

V

D

T

T

M

R

P

C

A

N

S

L

M

S

S

A

G

R

E

R

R

-

R

-

T

L

L

I

L

E

K

A

A

L

Y

G

G

S

S

Q

E

V

V

H

-

I

V

I

L

T

T

E

D

P

P

A

A

P

-

D

-

R

K

G

G

F

M

L

K

G

G

A

A

R

-

L

I

D

D

Y

M

V

A

H

N

S

Q

L

L

C

K

A

E

S

N

D

I

P

P

R

G

Y
x
S

V

I

W

I

L

L

N

A

Q

Q

Y

F

S

D

N

N

P

P

S

N

N

N

W

P

K

L

A

V

H

H

Y

Y

R

Q

R

T

T

T

A

G

P

P

E

E

I

I

A

W

R

R

Q

Q

F

T

P

K

-

G

N

T

L

V

D

D

V

A

L

V

F

V

I

F

G
|
G

T

V

G
|
G

T

T

T

G

G

G

T

T

L

I

M

T

G

G

C

V

A

G

R

R

Y

Y

F

L

R

Q

E

E

L

Q

G

N

S

P

P

G

V

V

R

R

I

V

V

F

A

A

V

V

D

E

S

P

V

E

G

E

S

S

V

A

T

I

F

L

G

S

G

G

E

R

P

P

-

A

G

G

R

P

R

H

M

K

I

I

P

Q

G

G

L

I

G
|
G

T

A

S

G

V

F

R

A

P

P

R

A

L

V

L

L

D

D

E

T

S

K

Y

I

V

Y

D

E

D

D

V

V

V

I

I

R

V

I

D

H

E

S

V

D

D

E

T

A

I

I

R

V

A

M

C

A

H

Q

R
|
R

L

L

A

S

-

Y

R

E

S

E

G

G

F

L

L

L

F

G

G

G

G

I

S

S

T
x
S

G

G

T

A

V

N

V

V

S

A

G

A

A

A

M

L

D

Q

W

L

L

A

A

A

R

R

H

P

G

E

S

M

P

A

G

G

M

K

T

L

S

I

V

V

A
x
T

I

C

A

L

P

P

D

S

L

C

G

G

E

E

R

R

Y

Y

L

M

D

T

T

S

L

P

Y

L

Q

Y

T

T

N

D

P75 (≠ S67) mutation to L: In n5537; severe loss of protein stability.
A88 (= A80) mutation to V: In n5522; severe loss of protein stability.
S144 (≠ Y140) mutation to F: In mr26; susceptible to high levels of hydrogen sulfide.
G181 (= G176) mutation to E: In n5521 and mr23; severe loss of protein stability. Susceptible to high levels of hydrogen sulfide.
G183 (= G178) mutation to R: In n5515; severe loss of protein stability.
G229 (= G223) mutation to E: In mr33; susceptible to high levels of hydrogen sulfide.
R259 (= R253) mutation to K: In n5519; no loss of protein stability. No effect on enzyme activity.
S272 (≠ T265) mutation to F: In mr29; susceptible to high levels of hydrogen sulfide.
T295 (≠ A288) mutation to I: In mr39; susceptible to high levels of hydrogen sulfide.

7n2tA O-acetylserine sulfhydrylase from citrullus vulgaris in the internal aldimine state, with citrate bound (see paper)
35% identity, 83% coverage: 30:301/328 of query aligns to 33:304/309 of 7n2tA

query
sites
7n2tA

K

K

C

L

E

E

G

M

F

M

N

E

F

P

A

C

G

S

S

S

I

V

K

K

L

D

K

R

A

I

A

G

I

Y

E

S

M

M

V

I

E

S

S

D

A

A

E

E

Q

N

H

K

G

G

L

L

V

I

T

T

P

P

-

G

D

E

T

S

I

V

L

L

V

I

E

E

S

P

S

T

S

S

G

G

N

N

L

T

G

G

V

I

A

G

L

L

A

A

I

F

I

I

A

A

S

A

K

K

G

G

Y

Y

E

R

F

L

L

I

C

I

V

C

T

M

D

P

T

A

R

S

C

M

N

S

L

L

S

E

A

R

R

R

R

T

L

I

I

L

E

R

A

A

L

F

G

G

S

A

Q

E

V

L

H

-

I

V

I

L

T

T

E

D

P

P

A

A

P

-

D

-

R

R

G

G

F

M

L

K

G

G

A

A

R

-

L

V

D

Q

Y

K

V

A

H

E

S

E

L

I

C

K

A

A

S

K

D

T

P

P

R

N

Y

S

V

Y

W

I

L

L

N

Q

Q

Q

Y

F

S

E

N

N

P

P

S

A

N

N

W

P

K

K

A

I

H

H

Y

Y

R

E

R

T

T

T

A

G

P

P

E

E

I

I

A

W

R

R

-

G

Q

S

F

G

P

G

N

K

L

I

D

D

V

A

L

L

F

V

I

S

G

G

T

I

G

G

T

T

T

G

G

G

T

T

L

V

M

T

G

G

C

A

A

G

R

K

Y

Y

F

L

R

K

E

E

L

Q

G

N

S

P

P

N

V

I

R

K

I

L

V

Y

A

G

V

V

D

E

S

P

V

V

G

E

S

S

V

A

T

I

F

L

-

S

G

G

E

K

P

P

G

G

R

P

R

H

M

K

I

I

P

Q

G

G

L

I

G

G

T

A

S

G

V

F

R

I

P

P

R

G

L

V

L

L

D

D

E

V

S

N

Y

L

V

L

D

D

D

E

V

V

V

I

I

Q

V

V

D

S

E

S

V

E

D

E

T

S

I

I

R

E

A

T

C

A

H

K

R

L

L

L

A

A

-

L

R

K

S

E

G

G

F

L

L

L

F

V

G

G

G

I

S

S

T

S

G

G

T

A

V

A

S

A

G

A

A

A

M

I

D
x
R

W

I

L

A

A

K

R
|
R

H

P

G

E

S

N

P

A

G

G

M

K

T

L

S

I

V

V

A

A

I

V

A

F

P

P

D

S

L

F

G

G

E

E

R

R

Y

Y

L

L

D

S

T

T

L

V

binding phosphate ion: R277 (≠ D274), R281 (= R278)

2efyA Crystal structure of t.Th. Hb8 o-acetylserine sulfhydrylase complexed with 4-acetylbutyric acid
36% identity, 84% coverage: 26:300/328 of query aligns to 25:298/302 of 2efyA

query
sites
2efyA

S

E

L

V

F

W

L

V

K

K

C

L

E

E

G

G

F

L

N

N

F

P

A

G

G

G

S

S

I

I

K
|
K

L

D

K

R

A

P

A

A

I

W

E

Y

M

M

V

I

E

K

S

D

A

A

E

E

Q

E

H

R

G

G

L

I

V

L

T

R

P

P

D

G

T

S

-

G

-

Q

I

V

L

I

V

V

E

E

S

P

S
x
T

S
|
S

G
|
G

N
|
N

L
x
T

G

G

V

I

A

G

L

L

A

A

I

M

I

I

A

A

S

S

K

R

G

G

Y

Y

E

R

F

L

L

I

C

L

V

T

T

M

D

P

T

A

R

Q

C

M

N

S

L

E

S

E

A

R

R

K

R

R

L

V

I

L

E

K

A

A

L

F

G

G

S

A

Q

E

V

L

H

-

I

V

I

L

T

T

E

D

P

P

A

-

P

-

D

E

R

R

G

R

F

M

L

L

G

A

A

A

R

R

-

E

-

A

L

L

D

R

Y

L

V

K

H

E

S

E

L

L

C

G

A

A

S

F

D

M

P

P

R

-

Y

-

V

-

W

-

L

-

N

D

Q
|
Q

Y

F

S

K

N

N

P

P

S

A

N

N

W

V

K

R

A

A

H

H

Y

Y

R

E

R

T

T

T

A

G

P

P

E

E

I

L

A

Y

R

E

Q

A

F

L

P

E

-

G

N

R

L

I

D

D

V

A

L

F

F

V

I
x
Y

G

G

T

S

G
|
G

T
|
T

T
x
G

G

G

T
|
T

L

I

M

T

G

G

C

V

A

G

R

R

Y

Y

F

L

R

K

E

E

L

R

G

I

S

P

P

H

V

V

R

K

I

V

V

I

A

A

V

V

D
x
E

S

P

V

A

G

R

S
|
S

-

N

V

V

T

L

F

S

G

G

E

K

P

M

G

G

R

Q

R

H

M

G

I

F

P

Q

G
|
G

L
x
M

G

G

T
x
P

S

G

V

F

R

I

P

P

R

E

L

N

L

L

D

D

E

L

S

S

Y

L

V

L

D

D

D

G

V

V

V

I

I

Q

V

V

D

W

E

E

V

E

D

D

T

A

I

F

R

P

A

L

C

A

H

R

R

R

L

L

A

A

R

R

S

E

-

E

G

G

F

L

L

F

F

L

G

G

G

M

S
|
S

T

S

G

G

T

G

V

I

V

V

S

W

G

A

A

A

M

L

D

Q

W

-

L

V

A

A

R

R

H

E

G

L

S

G

P

P

G

G

M

K

T

R

S

V

V

A

A

C

I

I

A

S

P
|
P

D
|
D

L

G

G

G

E

W

R

K

Y

Y

L

L

D

S

T

T

active site: K40 (= K41), S70 (= S69), E200 (≠ D203), S204 (= S207), S263 (= S264)
binding 5-oxohexanoic acid: T69 (≠ S68), G71 (= G70), T73 (≠ L72), Q141 (= Q145), G175 (= G178), G219 (= G221), M220 (≠ L222), P222 (≠ T224)
binding pyridoxal-5'-phosphate: K40 (= K41), N72 (= N71), Y172 (≠ I175), G175 (= G178), T176 (= T179), G177 (≠ T180), T179 (= T182), G219 (= G221), S263 (= S264), P289 (= P291), D290 (= D292)

2ecqA Crystal structure of t.Th. Hb8 o-acetylserine sulfhydrylase complexed with 3-hydroxylactate
36% identity, 84% coverage: 26:300/328 of query aligns to 25:298/302 of 2ecqA

query
sites
2ecqA

S

E

L

V

F

W

L

V

K

K

C

L

E

E

G

G

F

L

N

N

F

P

A

G

G

G

S

S

I

I

K
|
K

L

D

K

R

A

P

A

A

I

W

E

Y

M

M

V

I

E

K

S

D

A

A

E

E

Q

E

H

R

G

G

L

I

V

L

T

R

P

P

D

G

T

S

-

G

-

Q

I

V

L

I

V

V

E

E

S

P

S

T

S
|
S

G
|
G

N
|
N

L
x
T

G

G

V

I

A

G

L

L

A

A

I

M

I

I

A

A

S

S

K

R

G

G

Y

Y

E

R

F

L

L

I

C

L

V

T

T

M

D

P

T

A

R

Q

C

M

N

S

L

E

S

E

A

R

R

K

R

R

L

V

I

L

E

K

A

A

L

F

G

G

S

A

Q

E

V

L

H

-

I

V

I

L

T

T

E

D

P

P

A

-

P

-

D

E

R

R

G

R

F

M

L

L

G

A

A

A

R

R

-

E

-

A

L

L

D

R

Y

L

V

K

H

E

S

E

L

L

C

G

A

A

S

F

D

M

P

P

R

-

Y

-

V

-

W

-

L

-

N

D

Q
|
Q

Y

F

S

K

N

N

P

P

S

A

N

N

W

V

K

R

A

A

H

H

Y

Y

R

E

R

T

T

T

A

G

P

P

E

E

I

L

A

Y

R

E

Q

A

F

L

P

E

-

G

N

R

L

I

D

D

V

A

L

F

F

V

I
x
Y

G
|
G

T

S

G
|
G

T
|
T

T

G

G

G

T
|
T

L

I

M

T

G

G

C

V

A

G

R

R

Y

Y

F

L

R

K

E

E

L

R

G

I

S

P

P

H

V

V

R

K

I

V

V

I

A

A

V

V

D
x
E

S

P

V

A

G

R

S
|
S

-

N

V

V

T

L

F

S

G

G

E

K

P

M

G

G

R

Q

R

H

M

G

I

F

P

Q

G
|
G

L

M

G

G

T

P

S

G

V

F

R

I

P

P

R

E

L

N

L

L

D

D

E

L

S

S

Y

L

V

L

D

D

D

G

V

V

V

I

I

Q

V

V

D

W

E

E

V

E

D

D

T

A

I

F

R

P

A

L

C

A

H

R

R

R

L

L

A

A

R

R

S

E

-

E

G

G

F

L

L

F

F

L

G

G

G

M

S
|
S

T

S

G

G

T

G

V

I

V

V

S

W

G

A

A

A

M

L

D

Q

W

-

L

V

A

A

R

R

H

E

G

L

S

G

P

P

G

G

M

K

T

R

S

V

V

A

A

C

I

I

A

S

P
|
P

D

D

L

G

G

G

E

W

R

K

Y

Y

L

L

D

S

T

T

active site: K40 (= K41), S70 (= S69), E200 (≠ D203), S204 (= S207), S263 (= S264)
binding (3s)-3-hydroxybutanoic acid: K40 (= K41), G71 (= G70), T73 (≠ L72), Q141 (= Q145), G219 (= G221)
binding pyridoxal-5'-phosphate: K40 (= K41), N72 (= N71), Y172 (≠ I175), G173 (= G176), G175 (= G178), T176 (= T179), T179 (= T182), G219 (= G221), S263 (= S264), P289 (= P291)

2ecoA Crystal structure of t.Th. Hb8 o-acetylserine sulfhydrylase complexed with 4-methylvalerate
36% identity, 84% coverage: 26:300/328 of query aligns to 25:298/302 of 2ecoA

query
sites
2ecoA

S

E

L

V

F

W

L

V

K

K

C

L

E

E

G

G

F

L

N

N

F

P

A

G

G

G

S

S

I

I

K
|
K

L

D

K

R

A

P

A

A

I

W

E

Y

M

M

V

I

E

K

S

D

A

A

E

E

Q

E

H

R

G

G

L

I

V

L

T

R

P

P

D

G

T

S

-

G

-

Q

I

V

L

I

V

V

E

E

S

P

S
x
T

S
|
S

G
|
G

N
|
N

L
x
T

G

G

V

I

A

G

L

L

A

A

I

M

I

I

A

A

S

S

K

R

G

G

Y

Y

E

R

F

L

L

I

C

L

V

T

T

M

D

P

T

A

R

Q

C

M

N

S

L

E

S

E

A

R

R

K

R

R

L

V

I

L

E

K

A

A

L

F

G

G

S

A

Q

E

V

L

H

-

I

V

I

L

T

T

E

D

P

P

A

-

P

-

D

E

R

R

G

R

F

M

L

L

G

A

A

A

R

R

-

E

-

A

L

L

D

R

Y

L

V

K

H

E

S

E

L

L

C

G

A

A

S

F

D

M

P

P

R

-

Y

-

V

-

W

-

L

-

N

D

Q
|
Q

Y

F

S

K

N

N

P

P

S

A

N

N

W

V

K

R

A

A

H

H

Y

Y

R

E

R

T

T

T

A

G

P

P

E

E

I

L

A

Y

R

E

Q

A

F

L

P

E

-

G

N

R

L

I

D

D

V

A

L

F

F

V

I
x
Y

G

G

T

S

G
|
G

T
|
T

T

G

G

G

T
|
T

L

I

M

T

G

G

C

V

A

G

R

R

Y

Y

F

L

R

K

E

E

L

R

G

I

S

P

P

H

V

V

R

K

I

V

V

I

A

A

V

V

D
x
E

S

P

V

A

G

R

S
|
S

-

N

V

V

T

L

F

S

G

G

E

K

P

M

G

G

R

Q

R

H

M

G

I

F

P

Q

G
|
G

L

M

G

G

T

P

S

G

V

F

R

I

P

P

R

E

L

N

L

L

D

D

E

L

S

S

Y

L

V

L

D

D

D

G

V

V

V

I

I

Q

V

V

D

W

E

E

V

E

D

D

T

A

I

F

R

P

A

L

C

A

H

R

R

R

L

L

A

A

R

R

S

E

-

E

G

G

F

L

L

F

F

L

G

G

G

M

S
|
S

T

S

G

G

T

G

V

I

V

V

S

W

G

A

A

A

M

L

D

Q

W

-

L

V

A

A

R

R

H

E

G

L

S

G

P

P

G

G

M

K

T

R

S

V

V

A

A

C

I

I

A

S

P
|
P

D
|
D

L

G

G

G

E

W

R

K

Y

Y

L

L

D

S

T

T

active site: K40 (= K41), S70 (= S69), E200 (≠ D203), S204 (= S207), S263 (= S264)
binding 4-methyl valeric acid: K40 (= K41), T69 (≠ S68), G71 (= G70), T73 (≠ L72), Q141 (= Q145), G175 (= G178), T176 (= T179), G219 (= G221)
binding pyridoxal-5'-phosphate: K40 (= K41), N72 (= N71), Y172 (≠ I175), G175 (= G178), T176 (= T179), T179 (= T182), G219 (= G221), S263 (= S264), P289 (= P291), D290 (= D292)

Query Sequence

>WP_005457514.1 NCBI__GCF_000244975.1:WP_005457514.1
MPVITAPLEFNEEQLYVDLESMFGHSLFLKCEGFNFAGSIKLKAAIEMVESAEQHGLVTP
DTILVESSSGNLGVALAIIAASKGYEFLCVTDTRCNLSARRLIEALGSQVHIITEPAPDR
GFLGARLDYVHSLCASDPRYVWLNQYSNPSNWKAHYRRTAPEIARQFPNLDVLFIGTGTT
GTLMGCARYFRELGSPVRIVAVDSVGSVTFGGEPGRRMIPGLGTSVRPRLLDESYVDDVV
IVDEVDTIRACHRLARSGFLFGGSTGTVVSGAMDWLARHGSPGMTSVAIAPDLGERYLDT
LYQTNWLQGLYGEDVLNFAEIAAGVRPG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory