SitesBLAST
Comparing WP_005458675.1 NCBI__GCF_000244975.1:WP_005458675.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
3dwgA Crystal structure of a sulfur carrier protein complex found in the cysteine biosynthetic pathway of mycobacterium tuberculosis (see paper)
75% identity, 100% coverage: 1:316/316 of query aligns to 3:325/325 of 3dwgA
- active site: K53 (= K44), S267 (= S258)
- binding pyridoxal-5'-phosphate: K53 (= K44), N83 (= N74), G186 (= G177), T187 (= T178), T188 (= T179), G189 (= G180), T190 (= T181), S267 (= S258), A296 (= A287), D297 (= D288)
P9WP53 O-phosphoserine sulfhydrylase; OPS sulfhydrylase; CysO-thiocarboxylate-dependent cysteine synthase; Cysteine synthase B; CSase B; O-phosphoserine-specific cysteine synthase; [CysO sulfur-carrier protein]-thiocarboxylate-dependent cysteine synthase; EC 2.5.1.113 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 3 papers)
75% identity, 100% coverage: 1:316/316 of query aligns to 1:323/323 of P9WP53
- N81 (= N74) binding pyridoxal 5'-phosphate
- R220 (= R213) mutation to A: 700-fold decrease in the rate of the first half-reaction using OPS. Affects neither the rate of the first half-reaction using OAS nor the rate of the second half-reaction using sulfide or CysO-COSH.
- S265 (= S258) binding pyridoxal 5'-phosphate
- GQLWA 319:323 (= GQLWA 312:316) mutation Missing: Decreased lifetime of the alpha-aminoacrylate reaction intermediate, increased susceptibility to oxidation by oxidative agents such as hydrogen peroxide, and partial loss of selectivity towards CysO-COSH as sulfur donor.
5i7rA Mycobacterium tuberculosis cysm in complex with the urea-scaffold inhibitor 2 [3-(3-([1,1'-biphenyl]-3-yl)ureido)benzoic acid] (see paper)
71% identity, 99% coverage: 2:315/316 of query aligns to 1:306/306 of 5i7rA
- active site: K50 (= K44), S249 (= S258)
- binding 3-{[([1,1'-biphenyl]-3-yl)carbamoyl]amino}benzoic acid: K50 (= K44), T77 (= T71), S78 (= S72), N80 (= N74), T81 (= T75), Y151 (= Y145), G183 (= G177), P209 (= P203), S249 (= S258)
- binding pyridoxal-5'-phosphate: K50 (= K44), N80 (= N74), G183 (= G177), T184 (= T178), T185 (= T179), T187 (= T181), S249 (= S258), A278 (= A287), D279 (= D288)
5i7oB Mycobacterium tuberculosis cysm in complex with the urea-scaffold inhibitor 7 [3-(3-(4-chlorophenyl)ureido)benzoic acid] (see paper)
72% identity, 95% coverage: 2:302/316 of query aligns to 1:295/295 of 5i7oB
- active site: K50 (= K44), S251 (= S258)
- binding pyridoxal-5'-phosphate: K50 (= K44), N80 (= N74), G183 (= G177), T184 (= T178), T185 (= T179), T187 (= T181), S251 (= S258), A280 (= A287), D281 (= D288)
- binding 3-{[(4-chlorophenyl)carbamoyl]amino}benzoic acid: K50 (= K44), T77 (= T71), S78 (= S72), N80 (= N74), T81 (= T75), Q150 (= Q144), Y151 (= Y145), G183 (= G177), T184 (= T178), A207 (= A201), E208 (= E202)
5iw8A Mycobacterium tuberculosis cysm in complex with the urea-scaffold inhibitor 4 [5-(3-([1,1'-biphenyl]-3-yl)ureido)-2-hydroxybenzoic acid] (see paper)
71% identity, 96% coverage: 3:306/316 of query aligns to 1:295/299 of 5iw8A
- active site: K49 (= K44), S247 (= S258)
- binding 5-{[([1,1'-biphenyl]-3-yl)carbamoyl]amino}-2-hydroxybenzoic acid: K49 (= K44), G78 (= G73), N79 (= N74), T80 (= T75), P208 (= P203), A229 (= A240), S247 (= S258), A250 (= A261)
- binding pyridoxal-5'-phosphate: K49 (= K44), N79 (= N74), G182 (= G177), T183 (= T178), T184 (= T179), T186 (= T181), S247 (= S258), A276 (= A287), D277 (= D288)
5i7aB Mycobacterium tuberculosis cysm in complex with the urea-scaffold inhibitor 1 [3-(3-(3,4-dichlorophenyl)ureido)benzoic acid] (see paper)
72% identity, 94% coverage: 3:300/316 of query aligns to 1:292/292 of 5i7aB
- active site: K49 (= K44), S250 (= S258)
- binding 3-{[(3,4-dichlorophenyl)carbamoyl]amino}benzoic acid: K49 (= K44), T76 (= T71), S77 (= S72), N79 (= N74), T80 (= T75), Q149 (= Q144), Y150 (= Y145), G182 (= G177), T183 (= T178), A206 (= A201), E207 (= E202), P208 (= P203), S250 (= S258)
- binding pyridoxal-5'-phosphate: K49 (= K44), N79 (= N74), G180 (= G175), G182 (= G177), T183 (= T178), T184 (= T179), T186 (= T181), S250 (= S258), A279 (= A287), D280 (= D288)
5i7hB Mycobacterium tuberculosis cysm in complex with the urea-scaffold inhibitor 6 [3-(3-(4-bromophenyl)ureido)benzoic acid] (see paper)
72% identity, 95% coverage: 2:300/316 of query aligns to 1:291/291 of 5i7hB
- active site: K49 (= K44), S249 (= S258)
- binding 3-{[(4-bromophenyl)carbamoyl]amino}benzoic acid: K49 (= K44), T76 (= T71), S77 (= S72), N79 (= N74), T80 (= T75), Q149 (= Q144), Y150 (= Y145), G182 (= G177), T183 (= T178), A206 (= A201), E207 (= E202), P208 (= P203), S249 (= S258)
- binding pyridoxal-5'-phosphate: K49 (= K44), N79 (= N74), G182 (= G177), T183 (= T178), T184 (= T179), T186 (= T181), S249 (= S258), A278 (= A287), D279 (= D288)
5i6dB Mycobacterium tuberculosis cysm in complex with the urea-scaffold inhibitor 5 [3-(3-(p-tolyl)ureido) benzoic acid] (see paper)
72% identity, 94% coverage: 3:300/316 of query aligns to 1:287/287 of 5i6dB
- active site: K45 (= K44), S245 (= S258)
- binding 3-{[(4-methylphenyl)carbamoyl]amino}benzoic acid: K45 (= K44), T72 (= T71), S73 (= S72), N75 (= N74), T76 (= T75), Q145 (= Q144), Y146 (= Y145), G178 (= G177), T179 (= T178), E203 (= E202), P204 (= P203), S245 (= S258)
- binding pyridoxal-5'-phosphate: K45 (= K44), N75 (= N74), G178 (= G177), T179 (= T178), T180 (= T179), T182 (= T181), S245 (= S258), A274 (= A287), D275 (= D288)
5iwcA Mycobacterium tuberculosis cysm in complex with the urea-scaffold inhibitor 3 [4-(3-([1,1'-biphenyl]-3-yl)ureido)-2-hydroxybenzoic acid] (see paper)
70% identity, 98% coverage: 2:311/316 of query aligns to 1:297/297 of 5iwcA
- active site: K50 (= K44), S244 (= S258)
- binding 4-{[([1,1'-biphenyl]-3-yl)carbamoyl]amino}-2-hydroxybenzoic acid: K50 (= K44), T77 (= T71), T81 (= T75), Q145 (= Q144), P204 (= P203), A226 (= A240), S244 (= S258), A247 (= A261)
- binding pyridoxal-5'-phosphate: K50 (= K44), N80 (= N74), G178 (= G177), T179 (= T178), T180 (= T179), G181 (= G180), T182 (= T181), S244 (= S258), A273 (= A287), D274 (= D288)
5i6dA Mycobacterium tuberculosis cysm in complex with the urea-scaffold inhibitor 5 [3-(3-(p-tolyl)ureido) benzoic acid] (see paper)
70% identity, 95% coverage: 3:301/316 of query aligns to 1:285/285 of 5i6dA
- active site: K49 (= K44), S242 (= S258)
- binding 3-{[(4-methylphenyl)carbamoyl]amino}benzoic acid: K49 (= K44), T76 (= T71), S77 (= S72), N79 (= N74), T80 (= T75), Q142 (= Q144), Y143 (= Y145), G175 (= G177), A199 (= A201), E200 (= E202), P201 (= P203), S242 (= S258)
- binding pyridoxal-5'-phosphate: K49 (= K44), N79 (= N74), G175 (= G177), T176 (= T178), T177 (= T179), T179 (= T181), S242 (= S258), A271 (= A287), D272 (= D288)
5i7aA Mycobacterium tuberculosis cysm in complex with the urea-scaffold inhibitor 1 [3-(3-(3,4-dichlorophenyl)ureido)benzoic acid] (see paper)
70% identity, 94% coverage: 3:299/316 of query aligns to 1:281/281 of 5i7aA
- active site: K49 (= K44), S240 (= S258)
- binding 3-{[(3,4-dichlorophenyl)carbamoyl]amino}benzoic acid: K49 (= K44), T76 (= T71), S77 (= S72), N79 (= N74), T80 (= T75), Q141 (= Q144), Y142 (= Y145), G174 (= G177), A198 (= A201), E199 (= E202), P200 (= P203), S240 (= S258)
- binding pyridoxal-5'-phosphate: K49 (= K44), N79 (= N74), G174 (= G177), T175 (= T178), T176 (= T179), G177 (= G180), T178 (= T181), S240 (= S258), A269 (= A287), D270 (= D288)
5i7oA Mycobacterium tuberculosis cysm in complex with the urea-scaffold inhibitor 7 [3-(3-(4-chlorophenyl)ureido)benzoic acid] (see paper)
69% identity, 94% coverage: 3:299/316 of query aligns to 1:280/280 of 5i7oA
- active site: K49 (= K44), S239 (= S258)
- binding pyridoxal-5'-phosphate: K49 (= K44), N79 (= N74), G171 (= G175), L172 (= L176), G173 (= G177), T174 (= T178), T175 (= T179), T177 (= T181), S239 (= S258), A268 (= A287), D269 (= D288)
- binding 3-{[(4-chlorophenyl)carbamoyl]amino}benzoic acid: K49 (= K44), T76 (= T71), S77 (= S72), N79 (= N74), T80 (= T75), Q140 (= Q144), Y141 (= Y145), G173 (= G177), T174 (= T178), A197 (= A201), E198 (= E202), P199 (= P203), S239 (= S258)
5i7hA Mycobacterium tuberculosis cysm in complex with the urea-scaffold inhibitor 6 [3-(3-(4-bromophenyl)ureido)benzoic acid] (see paper)
69% identity, 94% coverage: 3:299/316 of query aligns to 1:280/280 of 5i7hA
- active site: K49 (= K44), S239 (= S258)
- binding 3-{[(4-bromophenyl)carbamoyl]amino}benzoic acid: K49 (= K44), T76 (= T71), S77 (= S72), N79 (= N74), T80 (= T75), Q140 (= Q144), Y141 (= Y145), G173 (= G177), T174 (= T178), A197 (= A201), E198 (= E202), P199 (= P203)
- binding pyridoxal-5'-phosphate: K49 (= K44), N79 (= N74), L172 (= L176), G173 (= G177), T174 (= T178), T175 (= T179), G176 (= G180), T177 (= T181), S239 (= S258), A268 (= A287), D269 (= D288)
P16703 Cysteine synthase B; CSase B; O-acetylserine (thiol)-lyase B; OAS-TL B; O-acetylserine sulfhydrylase B; EC 2.5.1.47 from Escherichia coli (strain K12) (see paper)
41% identity, 93% coverage: 5:299/316 of query aligns to 2:292/303 of P16703
- N71 (= N74) binding pyridoxal 5'-phosphate
- S255 (= S258) binding pyridoxal 5'-phosphate
2bhtA Crystal structure of o-acetylserine sulfhydrylase b (see paper)
40% identity, 93% coverage: 5:299/316 of query aligns to 2:292/294 of 2bhtA
- active site: K41 (= K44), S69 (= S72), Q199 (≠ E202), G203 (= G206), S255 (= S258), C280 (≠ A287)
- binding pyridoxal-5'-phosphate: K41 (= K44), N71 (= N74), M173 (≠ L176), G174 (= G177), T175 (= T178), T176 (= T179), T178 (= T181), G208 (= G211), S255 (= S258), C280 (≠ A287)
2efyA Crystal structure of t.Th. Hb8 o-acetylserine sulfhydrylase complexed with 4-acetylbutyric acid
44% identity, 92% coverage: 10:300/316 of query aligns to 6:302/302 of 2efyA
- active site: K40 (= K44), S70 (= S72), E200 (= E202), S204 (≠ G206), S263 (= S258)
- binding 5-oxohexanoic acid: T69 (= T71), G71 (= G73), T73 (= T75), Q141 (= Q144), G175 (= G177), G219 (≠ N214), M220 (≠ L215), P222 (≠ E217)
- binding pyridoxal-5'-phosphate: K40 (= K44), N72 (= N74), Y172 (≠ G174), G175 (= G177), T176 (= T178), G177 (≠ T179), T179 (= T181), G219 (≠ N214), S263 (= S258), P289 (≠ A287), D290 (= D288)
2ecqA Crystal structure of t.Th. Hb8 o-acetylserine sulfhydrylase complexed with 3-hydroxylactate
44% identity, 92% coverage: 10:300/316 of query aligns to 6:302/302 of 2ecqA
- active site: K40 (= K44), S70 (= S72), E200 (= E202), S204 (≠ G206), S263 (= S258)
- binding (3s)-3-hydroxybutanoic acid: K40 (= K44), G71 (= G73), T73 (= T75), Q141 (= Q144), G219 (≠ N214)
- binding pyridoxal-5'-phosphate: K40 (= K44), N72 (= N74), Y172 (≠ G174), G173 (= G175), G175 (= G177), T176 (= T178), T179 (= T181), G219 (≠ N214), S263 (= S258), P289 (≠ A287)
2ecoA Crystal structure of t.Th. Hb8 o-acetylserine sulfhydrylase complexed with 4-methylvalerate
44% identity, 92% coverage: 10:300/316 of query aligns to 6:302/302 of 2ecoA
- active site: K40 (= K44), S70 (= S72), E200 (= E202), S204 (≠ G206), S263 (= S258)
- binding 4-methyl valeric acid: K40 (= K44), T69 (= T71), G71 (= G73), T73 (= T75), Q141 (= Q144), G175 (= G177), T176 (= T178), G219 (≠ N214)
- binding pyridoxal-5'-phosphate: K40 (= K44), N72 (= N74), Y172 (≠ G174), G175 (= G177), T176 (= T178), T179 (= T181), G219 (≠ N214), S263 (= S258), P289 (≠ A287), D290 (= D288)
P9WP55 O-acetylserine sulfhydrylase; OAS sulfhydrylase; OASS; Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine-specific cysteine synthase; Sulfide-dependent cysteine synthase; EC 2.5.1.47 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
40% identity, 93% coverage: 11:304/316 of query aligns to 11:310/310 of P9WP55
- K44 (= K44) modified: N6-(pyridoxal phosphate)lysine
- N74 (= N74) binding pyridoxal 5'-phosphate
- GTGGT 178:182 (≠ GTTGT 177:181) binding pyridoxal 5'-phosphate
- S266 (= S258) binding pyridoxal 5'-phosphate
5xoqA Crystal structure of o-acetylserine sulfhydrylase with bound transcription factor peptide inhibitor from planctomyces limnophilus
41% identity, 92% coverage: 9:299/316 of query aligns to 10:306/310 of 5xoqA
- binding : T72 (= T71), S73 (= S72), G74 (= G73), T76 (= T75), M123 (≠ S122), Q144 (= Q144), R218 (vs. gap), H219 (vs. gap), Q222 (≠ R213), G223 (≠ N214), A226 (≠ E217)
Query Sequence
>WP_005458675.1 NCBI__GCF_000244975.1:WP_005458675.1
MARYASLLDALGGTPLVGLPRLSPSPEVRLWAKLEDRNPTGSIKDRPALAMIEAAERDGI
LKSGDTILEPTSGNTGISLAMAAKLKGYGMVCVMPENTSEERRQLLQAYGARIVYSPAAG
GSNEAVRRAKELAKANPDWVMLYQYGNPANADAHYYGTGPELLKDLPTLTHFVGGLGTTG
TLVGVGRYLREHKPDVQVIAAEPRYGELVYGLRNLDEGFVPELYDPEVLSGRYSVGAYDA
LRRTRELLEKEGIFAGISTGAVLHAALGVANKVLKKGGSADIAFVVADAGWKYLSTGAYS
GSLDEAAERLDGQLWA
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory