SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_005997848.1 NCBI__GCF_000143965.1:WP_005997848.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

1f3oA Crystal structure of mj0796 atp-binding cassette (see paper)
41% identity, 97% coverage: 6:235/236 of query aligns to 2:232/232 of 1f3oA

query
sites
1f3oA

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binding adenosine-5'-diphosphate: Y11 (= Y15), G41 (= G45), G43 (= G47), K44 (= K48), S45 (≠ T49), T46 (= T50)

1l2tA Dimeric structure of mj0796, a bacterial abc transporter cassette (see paper)
41% identity, 95% coverage: 6:229/236 of query aligns to 2:227/230 of 1l2tA

query
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1l2tA

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binding adenosine-5'-triphosphate: Y11 (= Y15), S40 (= S44), G41 (= G45), S42 (= S46), G43 (= G47), K44 (= K48), S45 (≠ T49), T46 (= T50), F138 (vs. gap), Q145 (= Q147), S147 (= S149), G149 (= G151), Q150 (≠ E152), H204 (= H206)

P75957 Lipoprotein-releasing system ATP-binding protein LolD; EC 7.6.2.- from Escherichia coli (strain K12) (see paper)
41% identity, 92% coverage: 6:223/236 of query aligns to 6:221/233 of P75957

query
sites
P75957

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G42 (= G42) mutation to D: Loss of lipoprotein release when overexpressed.

7arlD Lolcde in complex with lipoprotein and adp (see paper)
41% identity, 92% coverage: 6:223/236 of query aligns to 3:218/222 of 7arlD

query
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7arlD

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binding adenosine-5'-diphosphate: G42 (= G45), G44 (= G47), K45 (= K48), S46 (≠ T49)

7mdyC Lolcde nucleotide-bound
41% identity, 92% coverage: 6:223/236 of query aligns to 3:218/226 of 7mdyC

query
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7mdyC

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binding adp orthovanadate: Y12 (= Y15), G42 (= G45), S43 (= S46), G44 (= G47), K45 (= K48), S46 (≠ T49), T47 (= T50), Q91 (= Q96), H138 (≠ A143), E142 (≠ Q147), S144 (= S149), G145 (= G150), G146 (= G151), E168 (= E173), N172 (≠ P177), H201 (= H206)
binding magnesium ion: S46 (≠ T49), Q91 (= Q96)

5xu1B Structure of a non-canonical abc transporter from streptococcus pneumoniae r6 (see paper)
41% identity, 93% coverage: 6:224/236 of query aligns to 4:219/226 of 5xu1B

query
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5xu1B

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G

D

Q

D

E

V

V

I

A

Y

-

D

-

D

-

G

G

W

L

K

G

V

E

K

K

D

Q

L

L

R

A

A

K

L

V

R

R

R

N

D

Q

R

Q

I

I

G

G

F

F

V

V

F

F

Q

Q

A

Q

P

F

Y

F

L

L

I

L

P

S

F

K

L

L

D

N

V

A

T

L

D

Q

N

N

V

V

A

E

L

L

L

P

P

L

M

I

L

Y

A

A

G

G

M

V

P

S

N

S

A

S

E

K

A

R

R

R

K

K

R

L

A

A

I

E

G

E

L

Y

F

L

K

D

A

K

L

V

D

E

V

L

E

T

H

E

R

R

A

S

K

H

A

H

M

L

P

P

S

S

Q

E

L

L

S

S

G

G

G

G

E

Q

Q

K

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

G

A

L

L

V

V

N

N

R

N

P

P

P

S

V

I

I

I

L

L

A

A

D

D

E

E

P

P

T

T

A

G

P

A

L

L

D

D

S

T

E

K

R

T

A

G

L

N

A

Q

V

I

I

M

R

Q

I

L

L

L

N

V

D

D

M

L

A

N

K

K

K

E

F

G

E

K

T

T

A

-

I

I

I

I

V

M

V

V

T

T

H

H

D

E

E

P

K

E

I

I

I

A

P

A

T

Y

F

A

K

K

R

R

I

Q

Y

I

H

V

I

I

R

R

D

D

G

G

V

V

binding magnesium ion: G45 (= G47), K46 (= K48), S47 (≠ T49)

7v8iD Lolcd(e171q)e with bound amppnp in nanodiscs (see paper)
40% identity, 92% coverage: 6:223/236 of query aligns to 5:220/229 of 7v8iD

query
sites
7v8iD

I

L

R

Q

I

C

Q

D

G

N

L

L

K

C

K

K

R

R

Y

Y

G

Q

S

E

G

G

D

S

T

V

A

Q

V

T

D

D

A
x
V

L

L

K

H

T

N

V

V

D

S

M

F

H

S

V

V

A

G

P

E

G

G

E

E

V

M

V

M

G

A

L

I

I

V

G

G

P

S

S
|
S

G
|
G

S

S

G
|
G

K
|
K

T
x
S

T
|
T

L

L

L

L

K

H

C

L

L

L

G

G

A

G

V

L

I

D

E

T

P

P

T

T

A

S

G

G

K

-

M

-

I

-

L

-

G

-

D

-

D

D

V

V

I

I

Y

F

D

N

D

-

G

G

W

Q

K

P

V

M

K

S

D

K

L

L

R

S

A

S

-

A

-

A

-

K

-

A

-

E

L

L

R

R

R

N

D

Q

R

K

I

L

G

G

F

F

V

I

F

Y

Q
|
Q

A

F

P

H

Y

H

L

L

I

L

P

P

F

D

L

F

D

T

V

A

T

L

D

E

N

N

V

V

A

A

L

M

L

-

P

P

M

L

L

L

A

I

G

G

M

K

P

K

N

K

-

P

A

A

E

E

A

I

R

N

K

S

R

R

A

A

I

L

G

E

L

M

F

L

K

K

A

A

L

V

D

G

V

L

E

D

H

H

R
|
R

A

A

K

N

A
x
H

M

R

P

P

S

S

Q
x
E

L

L

S
|
S

G

G

G
|
G

E
|
E

Q

R

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

G

A

L

L

V

V

N

N

R

N

P

P

P

R

V

L

I

V

L

L

A

A

D

D

E

Q

P

P

T

T

A

G

P

N

L

L

D

D

S

A

E

R

R

N

A

A

L

D

A

S

V

I

I

F

R

Q

I

L

L

L

N

G

D

E

M

L

A

N

K

R

K

L

F

Q

E

G

T

T

A

A

I

F

I

L

V

V

V

V

T

T

H
|
H

D

D

E

L

K

Q

I

L

I

A

P

K

T

R

F

M

K

S

R

R

I

Q

Y

L

H

E

I

M

R

R

D

D

G

G

binding phosphoaminophosphonic acid-adenylate ester: V23 (≠ A24), S43 (= S44), G44 (= G45), G46 (= G47), K47 (= K48), S48 (≠ T49), T49 (= T50), Q93 (= Q96), R137 (= R140), H140 (≠ A143), E144 (≠ Q147), S146 (= S149), G148 (= G151), E149 (= E152), H203 (= H206)
binding magnesium ion: S48 (≠ T49), Q93 (= Q96)

9gvkD Cryo-em structure of endogenous atp-bound lolcde with lold-e171q mutations in nanodiscs (see paper)
40% identity, 92% coverage: 6:223/236 of query aligns to 4:219/226 of 9gvkD

query
sites
9gvkD

I

L

R

Q

I

C

Q

D

G

N

L

L

K

C

K

K

R

R

Y
|
Y

G

Q

S

E

G

G

D

S

T

V

A

Q

V

T

D

D

A

V

L

L

K

H

T

N

V

V

D

S

M

F

H

S

V

V

A

G

P

E

G

G

E

E

V

M

V

M

G

A

L

I

I

V

G

G

P

S

S
|
S

G
|
G

S

S

G
|
G

K
|
K

T
x
S

T
|
T

L

L

L

L

K

H

C

L

L

L

G

G

A

G

V

L

I

D

E

T

P

P

T

T

A

S

G

G

K

-

M

-

I

-

L

-

G

-

D

-

D

D

V

V

I

I

Y

F

D

N

D

-

G

G

W

Q

K

P

V

M

K

S

D

K

L

L

R

S

A

S

-

A

-

A

-

K

-

A

-

E

L

L

R

R

R

N

D

Q

R

K

I

L

G

G

F

F

V

I

F

Y

Q
|
Q

A

F

P

H

Y

H

L

L

I

L

P

P

F

D

L

F

D

T

V

A

T

L

D

E

N

N

V

V

A

A

L

M

L

-

P

P

M

L

L

L

A

I

G

G

M

K

P

K

N

K

-

P

A

A

E

E

A

I

R

N

K

S

R

R

A

A

I

L

G

E

L

M

F

L

K

K

A

A

L

V

D

G

V

L

E

D

H

H

R
|
R

A

A

K

N

A
x
H

M

R

P

P

S

S

Q
x
E

L

L

S
|
S

G
|
G

G
|
G

E
|
E

Q

R

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

G

A

L

L

V

V

N

N

R

N

P

P

P

R

V

L

I

V

L

L

A

A

D

D

E

Q

P

P

T

T

A

G

P

N

L

L

D

D

S

A

E

R

R

N

A

A

L

D

A

S

V

I

I

F

R

Q

I

L

L

L

N

G

D

E

M

L

A

N

K

R

K

L

F

Q

E

G

T

T

A

A

I

F

I

L

V

V

V

V

T

T

H

H

D

D

E

L

K

Q

I

L

I

A

P

K

T

R

F

M

K

S

R

R

I

Q

Y

L

H

E

I

M

R

R

D

D

G

G

binding adenosine-5'-triphosphate: Y13 (= Y15), S42 (= S44), G43 (= G45), G45 (= G47), K46 (= K48), S47 (≠ T49), T48 (= T50), Q92 (= Q96), R136 (= R140), H139 (≠ A143), E143 (≠ Q147), S145 (= S149), G146 (= G150), G147 (= G151), E148 (= E152)
binding magnesium ion: S47 (≠ T49), Q92 (= Q96)

P75831 Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- from Escherichia coli (strain K12) (see paper)
39% identity, 92% coverage: 6:223/236 of query aligns to 5:219/648 of P75831

query
sites
P75831

I

L

R

E

I

L

Q

K

G

D

L

I

K

R

K

R

R

S

Y

Y

G

P

S

A

G

G

D

D

T

E

A

Q

V

V

D

E

A

V

L

L

K

K

T

G

V

I

D

S

M

L

H

D

V

I

A

Y

P

A

G

G

E

E

V

M

V

V

G

A

L

I

I

V

G

G

P

A

S

S

G

G

S

S

G

G

K
|
K

T

S

T

T

L

L

L

M

K

N

C

I

L

L

G

G

A

C

V

L

I

D

E

K

P

A

T

T

A

S

G

G

K

T

M

Y

-

R

I

V

L

A

G

G

D

Q

D

D

V

V

I

A

Y

T

D

L

D

D

G

A

W

-

K

-

V

-

K

D

D

A

L

L

R

A

A

Q

L

L

R

R

R

R

D

E

R

H

I

F

G

G

F

F

V

I

F

F

Q

Q

A

R

P

Y

Y

H

L

L

I

L

P

S

F

H

L

L

D

T

V

A

T

E

D

Q

N

N

V

V

A

E

L

V

L

P

P

A

M

V

L

Y

A

A

G

G

M

L

P

E

N

R

A

K

E

Q

A

R

R

L

K

L

R

R

A

A

I

Q

G

E

L

L

F

L

K

Q

A

R

L

L

D

G

V

L

E

E

H

D

R

R

A

T

K

E

A

Y

M

Y

P

P

S

A

Q

Q

L

L

S

S

G

G

G

G

E

Q

Q

Q

Q

Q

R

R

V

V

A

S

I

I

A

A

R

R

G

A

L

L

V

M

N

N

R

G

P

G

P

Q

V

V

I

I

L

L

A

A

D
|
D

E

E

P

P

T

T

A

G

P

A

L

L

D

D

S

S

E

H

R

S

A

G

L

E

A

E

V

V

I

M

R

A

I

I

L

L

N

H

D

Q

M

L

A

R

K

D

K

R

F

G

E

H

T

T

A

-

I

V

I

I

V

I

V

V

T

T

H

H

D

D

E

P

K

Q

I

V

I

A

P

A

T

Q

F

A

K

E

R

R

I

V

Y

I

H

E

I

I

R

R

D

D

G

G

K47 (= K48) mutation to L: Lack of activity.
D169 (= D172) mutation to N: Lack of activity.

5lilA Structure of aggregatibacter actinomycetemcomitans macb bound to atpys (p21) (see paper)
39% identity, 92% coverage: 6:223/236 of query aligns to 4:218/615 of 5lilA

query
sites
5lilA

I

I

R

E

I

I

Q

K

G

Q

L

L

K

N

K

R

R

Y

Y
x
F

G

G

S

E

G

G

D

E

T

N

A

R

V

V

D

H

A
x
V

L

L

K

K

T

D

V

I

D

S

M

L

H

S

V

I

A

E

P

R

G

G

E

D

V

F

V

V

G

A

L

I

I

M

G

G

P

Q

S
|
S

G
|
G

S

S

G
|
G

K
|
K

T
x
S

T
|
T

L

L

L

M

K

N

C

I

L

I

G

G

A

C

V

L

I

D

E

T

P

A

T

T

A

G

G

G

K

S

M

S

-

K

I

I

L

D

G

G

D

K

D

E

V

T

I

I

Y

E

D

L

D

T

G

N

W

D

K

Q

V

L

K

S

D

D

L

L

R

R

A

S

L

-

R

-

R

-

D

Q

R

K

I

F

G

G

F

F

V

I

F

F

Q
|
Q

A

R

P

Y

Y

N

L

L

I

L

P

S

F

S

L

L

D

T

V

A

T

A

D

E

N

N

V

V

A

A

L

L

L

P

P

A

M

I

L

Y

A

A

G

G

M

M

P

P

N

Q

A

S

E

Q

A

R

R

L

K

E

R

R

A

A

I

K

G

Q

L

L

F

L

K

E

A

K

L

L

D

G

V

L

E

G

H

D

R
x
K

A

W

K

Q

A

N

M

K

P

P

S

N

Q
|
Q

L

L

S
|
S

G

G

G
|
G

E
x
Q

Q

Q

Q

Q

R

R

V

V

A

S

I

I

A

A

R

R

G

A

L

L

V

M

N

N

R

G

P

G

P

E

V

I

I

I

L

L

A

A

D

D

E

Q

P

P

T

T

A

G

P

A

L

L

D

D

S

S

E

H

R

S

A

G

L

E

A

N

V

V

I

M

R

E

I

I

L

L

N

R

D

Q

M

L

A

H

K

E

K

E

F

G

E

H

T

T

A

-

I

I

I

I

V

M

V

V

T

T

H

H

D

D

E

K

K

H

I

I

I

A

P

A

T

S

F

A

K

N

R

R

I

I

Y

I

H

E

I

I

R

K

D

D

G

G

binding adenosine-5'-triphosphate: F13 (≠ Y15), V22 (≠ A24), S42 (= S44), G43 (= G45), G45 (= G47), K46 (= K48), S47 (≠ T49), T48 (= T50), Q92 (= Q96), K136 (≠ R140), Q143 (= Q147), S145 (= S149), G147 (= G151), Q148 (≠ E152)
binding magnesium ion: S47 (≠ T49), Q92 (= Q96)

5lj7A Structure of aggregatibacter actinomycetemcomitans macb bound to atp (p21) (see paper)
39% identity, 92% coverage: 6:223/236 of query aligns to 4:218/592 of 5lj7A

query
sites
5lj7A

I

I

R

E

I

I

Q

K

G

Q

L

L

K

N

K

R

R

Y

Y

F

G

G

S

E

G

G

D

E

T

N

A

R

V

V

D

H

A
x
V

L

L

K

K

T

D

V

I

D

S

M

L

H

S

V

I

A

E

P

R

G

G

E

D

V

F

V

V

G

A

L

I

I

M

G

G

P

Q

S
|
S

G
|
G

S

S

G
|
G

K
|
K

T
x
S

T
|
T

L

L

L

M

K

N

C

I

L

I

G

G

A

C

V

L

I

D

E

T

P

A

T

T

A

G

G

G

K

S

M

S

-

K

I

I

L

D

G

G

D

K

D

E

V

T

I

I

Y

E

D

L

D

T

G

N

W

D

K

Q

V

L

K

S

D

D

L

L

R

R

A

S

L

-

R

-

R

-

D

Q

R

K

I

F

G

G

F

F

V

I

F

F

Q
|
Q

A

R

P

Y

Y

N

L

L

I

L

P

S

F

S

L

L

D

T

V

A

T

A

D

E

N

N

V

V

A

A

L

L

L

P

P

A

M

I

L

Y

A

A

G

G

M

M

P

P

N

Q

A

S

E

Q

A

R

R

L

K

E

R

R

A

A

I

K

G

Q

L

L

F

L

K

E

A

K

L

L

D

G

V

L

E

G

H

D

R
x
K

A

W

K

Q

A

N

M

K

P

P

S

N

Q
|
Q

L

L

S
|
S

G

G

G
|
G

E
x
Q

Q

Q

Q

Q

R

R

V

V

A

S

I

I

A

A

R

R

G

A

L

L

V

M

N

N

R

G

P

G

P

E

V

I

I

I

L

L

A

A

D

D

E

Q

P

P

T

T

A

G

P

A

L

L

D

D

S

S

E

H

R

S

A

G

L

E

A

N

V

V

I

M

R

E

I

I

L

L

N

R

D

Q

M

L

A

H

K

E

K

E

F

G

E

H

T

T

A

-

I

I

I

I

V

M

V

V

T

T

H

H

D

D

E

K

K

H

I

I

I

A

P

A

T

S

F

A

K

N

R

R

I

I

Y

I

H

E

I

I

R

K

D

D

G

G

binding adenosine-5'-triphosphate: V22 (≠ A24), S42 (= S44), G43 (= G45), G45 (= G47), K46 (= K48), S47 (≠ T49), T48 (= T50), Q92 (= Q96), K136 (≠ R140), Q143 (= Q147), S145 (= S149), G147 (= G151), Q148 (≠ E152)
binding magnesium ion: S47 (≠ T49), Q92 (= Q96)

5ws4A Crystal structure of tripartite-type abc transporter macb from acinetobacter baumannii (see paper)
39% identity, 92% coverage: 6:223/236 of query aligns to 5:219/650 of 5ws4A

query
sites
5ws4A

I

L

R

E

I

V

Q

S

G

N

L

L

K

V

K

R

R

E

Y

F

G

P

S

A

G

G

D

E

T

S

A

T

V

I

D

Q

A

I

L

L

K

K

T

G

V

I

D

D

M

L

H

T

V

I

A

Y

P

E

G

G

E

E

V

L

V

V

G

A

L

I

I

V

G

G

P

Q

S
|
S

G
|
G

S

S

G

G

K

K

T
x
S

T

T

L

L

L

M

K

N

C

I

L

L

G

G

A

C

V

L

I

D

E

R

P

P

T

T

A

S

G

G

K

S

M

Y

I

-

L

-

G

-

D

-

D

K

V

V

I

N

Y

G

D

Q

D

E

G

T

W

G

K

K

V

L

K

E

-

P

-

D

D

Q

L

L

R

A

A

Q

L

L

R

R

R

R

D

E

R

Y

I

F

G

G

F

F

V

I

F

F

Q

Q

A

R

P

Y

Y

H

L

L

I

L

P

G

F

D

L

L

D

S

V

A

T

E

D

G

N

N

V

V

A

E

L

V

L

P

P

A

M

V

L

Y

A

A

G

G

M

V

P

T

N

P

A

A

E

D

A

R

R

K

K

Q

R

R

A

A

I

T

G

A

L

L

F

L

K

T

A

E

L

L

D

G

V

L

E

G

H

T

R

K

A

T

K

Q

A

N

M

R

P

P

S

S

Q

Q

L

L

S

S

G

G

G

G

E

Q

Q

Q

Q

Q

R

R

V

V

A

S

I

I

A

A

R

R

G

A

L

L

V

M

N

N

R

G

P

G

P

D

V

V

I

I

L

L

A

A

D

D

E

E

P

P

T

T

A

G

P

A

L

L

D

D

S

S

E

H

R

S

A

G

L

V

A

E

V

V

I

M

R

R

I

I

L

L

N

R

D

E

M

L

A

N

K

A

K

A

F

G

E

H

T

T

A

-

I

I

I

I

V

L

V

V

T

T

H

H

D

D

E

M

K

Q

I

V

I

A

P

K

T

N

F

A

K

T

R

R

I

I

Y

I

H

E

I

I

R

S

D

D

G

G

binding 5'-o-[(r)-hydroxy(thiophosphonooxy)phosphoryl]adenosine: S43 (= S44), G44 (= G45), S48 (≠ T49)

2pclA Crystal structure of abc transporter with complex (aq_297) from aquifex aeolicus vf5
42% identity, 85% coverage: 23:223/236 of query aligns to 17:214/223 of 2pclA

query
sites
2pclA

D

E

A

I

L

L

K

K

T

G

V

I

D

S

M

L

H

S

V

V

A

K

P

K

G

G

E

E

V

F

V

V

G

S

L

I

I

I

G

G

P

A

S

S

G

G

S

S

G

G

K

K

T

S

T

T

L

L

L

L

K

Y

C

I

L

L

G

G

A

L

V

L

I

D

E

A

P

P

T

T

A

E

G

G

K

K

M

V

I

F

L

L

-

E

G

G

D

K

D

E

V

V

I

D

Y

Y

D

T

D

N

G

E

W

-

K

-

V

-

K

K

D

E

L

L

R

S

A

L

L

L

R

R

R

N

D

R

R

K

I

L

G

G

F

F

V

V

F

F

Q

Q

A

F

P

H

Y

Y

L

L

I

I

P

P

F

E

L

L

D

T

V

A

T

L

D

E

N

N

V

V

A

-

L

I

L

V

P

P

M

M

L

L

A

K

-

M

G

G

M

K

P

P

N

K

A

K

E

E

A

A

R

K

K

E

R

R

A

G

I

E

G

Y

L

L

F

L

K

S

A

E

L

L

D

G

V

L

E

G

H

D

R

K

A

L

K

S

A

R

M

K

P

P

S

Y

Q

E

L

L

S

S

G

G

G

G

E

E

Q

Q

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

G

A

L

L

V

A

N

N

R

E

P

P

P

I

V

L

I

L

L

F

A

A

D

D

E
|
E

P
|
P

T
|
T

A

G

P

N

L

L

D

D

S

S

-

A

-

N

-

T

E

K

R

R

A

V

L

M

A

D

V

I

I

F

R

L

I

K

L

I

N

N

D

E

M

G

A

G

K

-

K

-

F

-

E

-

T

T

A

S

I

I

I

V

V

M

V

V

T
|
T

H

H

D

E

E

R

K

E

I

L

I

A

P

E

T

L

F

T

K

H

R

R

I

T

Y

L

H

E

I

M

R

K

D

D

G

G

binding magnesium ion: E165 (= E173), P166 (= P174), T167 (= T175), T196 (= T205)

P9WQK5 Uncharacterized ABC transporter ATP-binding protein Rv0073 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
38% identity, 91% coverage: 6:220/236 of query aligns to 4:215/330 of P9WQK5

query
sites
P9WQK5

I

L

R

S

I

I

Q

Q

G

N

L

L

K

V

K

V

R

E

Y

Y

G

Y

S

S

G

G

D

G

T

Y

A

A

V

L

D

R

A

P

L

I

K

N

T

G

V

L

D

N

M

L

H

D

V

V

A

A

P

A

G

G

E

S

V

L

V

V

G

M

L

L

I

L

G

G

P

P

S

S

G

G

S

C

G

G

K

K

T

T

T

T

L

L

L

L

K

S

C

C

L

L

G

G

A

G

V

I

I

L

E

R

P

P

T

K

A

S

G

G

K

A

M

I

I
x
K

L

F

G

D

D

E

-

V

D

D

V

I

I

T

Y

T

D

L

D

Q

G

G

W

A

K

E

V

L

K

A

D

N

L

Y

R

R

A

-

L

-

R

-

R

R

D

N

R

K

I

V

G

G

F

I

V

V

F

F

Q

Q

A

A

P

F

Y

N

L

L

I

V

P

P

F

S

L

L

D

T

V

A

T

V

D

E

N

N

V

V

A

M

L

V

L

P

P

L

M

R

L

S

A

A

G

G

M

M

P

S

N

R

A

R

E

A

A

S

R

R

K

R

R

R

A

A

I

E

G

E

L

L

F

L

K

A

A

R

L

V

D

N

V

L

E

A

H

E

R

R

A

M

K

N

A

H

M

R

P

P

S

G

Q

D

L

L

S

S

G

G

G

G

E

Q

Q

Q

Q

Q

R

R

V

V

A

A

I

V

A

A

R

R

G

A

L

I

V

A

N

L

R

D

P

P

P

P

V

L

I

I

L

L

A

A

D

D

E

E

P

P

T

T

A

A

P

H

L

L

D

D

S

F

E

I

R

Q

A

V

L

E

A

E

V

V

I

L

R

R

I

L

L

I

N

R

D

E

M

L

A

A

K

D

K

G

F

-

E

E

T

R

A

V

I

V

I

V

V

V

V

A

T

T

H

H

D

D

E

S

K

R

I

M

I

L

P

P

T

M

F

A

K

D

R

R

I

V

Y

V

H

E

I

L

K65 (≠ I67) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

4u00A Crystal structure of ttha1159 in complex with adp (see paper)
41% identity, 86% coverage: 6:207/236 of query aligns to 3:195/241 of 4u00A

query
sites
4u00A

I

I

R

R

I

I

Q

R

G

N

L

L

K

H

K

K

R

W

Y
x
F

G

G

S

P

G

-

D

-

T

-

A

-

V

L

D

H

A
x
V

L

L

K

K

T

G

V

I

D

H

M

L

H

E

V

V

A

A

P

P

G

G

E

E

V

K

V

L

G

V

L

I

I

I

G

G

P

P

S
|
S

G
|
G

S
|
S

G
|
G

K
|
K

T
x
S

T
|
T

L

L

L

I

K

R

C

T

L

I

G

N

A

R

V

L

I

E

E

D

P

F

T

Q

A

E

G

G

K

E

M

V

I

V

L

V

G

-

D

-

D

-

V

-

I

-

Y

-

D

-

D

D

G

G

W

L

K

S

V

V

K

K

D

D

L

D

R

R

A

A

L

L

R

R

-

E

-

I

R

R

D

R

R

E

I

V

G

G

F

M

V

V

F

F

Q

Q

A

Q

P

F

Y

N

L

L

I

F

P

P

F

H

L

M

D

T

V

V

T

L

D

E

N

N

V

V

A

T

L

L

L

A

P

P

M

M

-

R

L

V

A

R

G

R

M

W

P

P

N

R

A

E

E

K

A

A

R

E

K

K

R

K

A

A

I

L

G

E

L

L

F

L

K

E

A

R

L

V

D

G

V

I

E

L

H

D

R

Q

A

A

K

R

A

K

M

Y

P

P

S

A

Q

Q

L

L

S

S

G

G

G

G

E

Q

Q

Q

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

G

A

L

L

V

A

N

M

R

E

P

P

P

K

V

I

I

M

L

L

A

F

D

D

E

E

P

P

T

T

A

S

P

A

L

L

D

D

S

P

E

E

R

M

A

V

L

G

A

E

V

V

I

L

R

D

I

V

L

M

N

R

D

D

M

L

A

A

K

Q

K

G

F

G

E

M

T

T

A

-

I

M

I

V

V

V

V

V

T

T

H

H

D

E

binding adenosine-5'-diphosphate: F12 (≠ Y15), V17 (≠ A24), S37 (= S44), G38 (= G45), S39 (= S46), G40 (= G47), K41 (= K48), S42 (≠ T49), T43 (= T50)

8g4cB Bceabs atpgs high res tm (see paper)
33% identity, 94% coverage: 11:232/236 of query aligns to 9:228/248 of 8g4cB

query
sites
8g4cB

L

I

K

R

K

K

R

S

Y
|
Y

G

G

S

N

G

K

D

L

T

N

A

K

V

Q

D

E

A

V

L

L

K

K

T

G

V

I

D

D

M

I

H

H

V

I

A

E

P

K

G

G

E

E

V

F

V

V

G

S

L

I

I

M

G

G

P

A

S
|
S

G

G

S
|
S

G

G

K

K

T
|
T

T

T

L

L

L

L

K

N

C

V

L

L

G

S

A

S

V

I

I

D

E

Q

P

V

T

S

A

H

G

G

K

T

M

I

-

H

I

I

L

N

G

G

D

N

D

D

V

M

I

T

Y

-

D

-

D

-

G

A

W

M

K

K

V

E

K

K

D

Q

L

L

R

A

A

E

L

F

R

R

R

K

D

Q

R

H

I

L

G

G

F

F

V

I

F

F

Q

Q

A

D

P

Y

Y

N

L

L

I

L

P

D

F

T

L

L

D

T

V

V

T

K

D

E

N

N

V

I

A

L

L

L

L

P

P

L

M

S

L

I

A

T

G

K

M

L

P

S

N

K

A

K

E

E

A

A

R

N

K

R

R

K

A

F

I

E

G

E

L

V

F

A

K

K

A

E

L

L

D

G

V

I

E

Y

H

E

R

L

A

R

K

D

A

K

M

Y

P

P

S

N

Q

E

L

I

S

S

G

G

G

G

E

Q

Q

K

Q

Q

R

R

V

T

A

S

I

A

A

G

R

R

G

A

L

F

V

I

N

H

R

D

P

P

P

S

V

I

I

I

L

F

A

A

D
|
D

E

E

P

P

T

T

A

G

P

A

L

L

D

D

S

S

E

K

R

S

A

A

L

S

A

D

V

L

I

L

R

N

I

K

L

L

N

S

D

Q

M

L

A

N

K

Q

K

K

F

R

E

N

T

A

A

T

I

I

I

I

V

M

V

V

T

T

H

H

D

D

E

P

K

V

I

A

I

A

P

S

T

Y

F

C

K

G

R

R

I

V

Y

I

H

F

I

I

R

K

D

D

G

G

V

Q

T

M

Y

Y

E

T

E

Q

E

L

G

N

E

K

G

G

binding phosphothiophosphoric acid-adenylate ester: Y13 (= Y15), S42 (= S44), S44 (= S46), T47 (= T49), D168 (= D172)

7tchB Bceab e169q variant atp-bound conformation (see paper)
33% identity, 94% coverage: 11:232/236 of query aligns to 8:227/245 of 7tchB

query
sites
7tchB

L

I

K

R

K

K

R

S

Y
|
Y

G

G

S

N

G

K

D

L

T

N

A

K

V
x
Q

D

E

A
x
V

L

L

K

K

T

G

V

I

D

D

M

I

H

H

V

I

A

E

P

K

G

G

E

E

V

F

V

V

G

S

L

I

I

M

G

G

P

A

S
|
S

G
|
G

S
|
S

G

G

K
|
K

T
|
T

T

T

L

L

L

L

K

N

C

V

L

L

G

S

A

S

V

I

I

D

E

Q

P

V

T

S

A

H

G

G

K

T

M

I

-

H

I

I

L

N

G

G

D

N

D

D

V

M

I

T

Y

-

D

-

D

-

G

A

W

M

K

K

V

E

K

K

D

Q

L

L

R

A

A

E

L

F

R

R

R

K

D

Q

R

H

I

L

G

G

F

F

V

I

F

F

Q

Q

A

D

P

Y

Y

N

L

L

I

L

P

D

F

T

L

L

D

T

V

V

T

K

D

E

N

N

V

I

A

L

L

L

L

P

P

L

M

S

L

I

A

T

G

K

M

L

P

S

N

K

A

K

E

E

A

A

R

N

K

R

R

K

A

F

I

E

G

E

L

V

F

A

K

K

A

E

L

L

D

G

V

I

E

Y

H

E

R

L

A

R

K

D

A

K

M

Y

P

P

S

N

Q
x
E

L

I

S

S

G
|
G

G
|
G

E

Q

Q

K

Q

Q

R

R

V

T

A

S

I

A

A

G

R

R

G

A

L

F

V

I

N

H

R

D

P

P

P

S

V

I

I

I

L

F

A

A

D

D

E

Q

P

P

T

T

A

G

P

A

L

L

D

D

S

S

E

K

R

S

A

A

L

S

A

D

V

L

I

L

R

N

I

K

L

L

N

S

D

Q

M

L

A

N

K

Q

K

K

F

R

E

N

T

A

A

T

I

I

I

I

V

M

V

V

T

T

H

H

D

D

E

P

K

V

I

A

I

A

P

S

T

Y

F

C

K

G

R

R

I

V

Y

I

H

F

I

I

R

K

D

D

G

G

V

Q

T

M

Y

Y

E

T

E

Q

E

L

G

N

E

K

G

G

binding adenosine-5'-triphosphate: Y12 (= Y15), Q19 (≠ V22), V21 (≠ A24), S41 (= S44), G42 (= G45), S43 (= S46), K45 (= K48), T46 (= T49), E142 (≠ Q147), G145 (= G150), G146 (= G151)

8tzjA Cryo-em structure of vibrio cholerae ftse/ftsx complex (see paper)
39% identity, 91% coverage: 6:220/236 of query aligns to 3:210/220 of 8tzjA

query
sites
8tzjA

I

I

R

R

I

F

Q

Q

G

Q

L

V

K

S

K

K

R

A

Y
|
Y

G

R

S

G

G

G

D
x
R

T

-

A

-

V

-

D

Q

A
|
A

L

L

K

Q

T

K

V

V

D

D

M

F

H

H

V

L

A

R

P

R

G

G

E

E

V

M

V

A

G

F

L

L

I

G

G

G

P

H

S

S

G
|
G

S

A

G
|
G

K
|
K

T
x
S

T

T

L

L

L

L

K

K

C

L

L

I

G

C

A

A

V

I

I

E

E

R

P

P

T

T

A

D

G

G

K

K

M

I

I

S

L

F

-

N

G

G

D

H

D

D

V

I

I

T

Y

R

D

-

D

-

G

-

W

I

K

P

V

N

K

K

D

D

L

I

R

P

A

F

L

L

R

R

R

R

D

N

R

-

I

I

G

G

F

I

V

V

F

F

Q

Q

A

D

P

H

Y

R

L

L

I

L

P

M

F

D

L

R

D

S

V

I

T

Y

D

D

N

N

V

V

A

A

L

L

L

P

P

M

M

R

L

I

A

E

G

S

M

I

P

S

N

E

A

N

E

E

A

I

R

K

K

R

R

R

A

V

I

S

G

A

L

A

F

L

K

D

A

K

L

T

D

G

V

L

E

L

H

D

R

K

A

A

K

R

A

C

M

L

P

P

S

S

Q

Q

L

L

S

S

G

G

G

G

E

E

Q

Q

Q

Q

R

R

V

V

A

G

I

I

A

A

R

R

G

A

L

V

V

V

N

N

R

R

P

P

P

T

V

L

I

L

L

L

A

A

D

D

E

E

P

P

T

T

A

G

P

N

L

L

D

D

S

P

E

E

R

L

A

S

L

S

A

R

V

V

I

L

R

R

I

L

L

F

N

E

D

E

M

F

A

N

K

R

K

A

F

G

E

V

T

T

A

-

I

I

I

L

V

L

V

A

T

T

H

H

D

D

E

I

K

H

I

L

I

V

P

N

T

S

F

R

K

P

R

Q

I

Y

Y

R

H

H

I

L

binding adenosine-5'-diphosphate: Y12 (= Y15), R16 (≠ D19), A18 (= A24), G39 (= G45), G41 (= G47), K42 (= K48), S43 (≠ T49)

P0A9R7 Cell division ATP-binding protein FtsE from Escherichia coli (strain K12) (see paper)
37% identity, 94% coverage: 6:227/236 of query aligns to 2:222/222 of P0A9R7

query
sites
P0A9R7

I

I

R

R

I

F

Q

E

G

H

L

V

K

S

K

K

R

A

Y

Y

G

L

S

G

G

G

D

R

T

-

A

-

V

-

D

Q

A

A

L

L

K

Q

T

G

V

V

D

T

M

F

H

H

V

M

A

Q

P

P

G

G

E

E

V

M

V

A

G

F

L

L

I

T

G

G

P

H

S

S

G

G

S

A

G

G

K
|
K

T

S

T

T

L

L

L

L

K

K

C

L

L

I

G
x
C

A

G

V

I

I

E

E

R

P

P

T

S

A

A

G

G

K

K

M

I

I

W

L

F

-

S

G

G

D

H

D

D

V

I

I

T

Y

R

D

-

D

-

G

-

W

L

K

K

V

N

K

R

D

E

L

V

R

P

A

F

L

L

R

R

R

R

D

-

R

Q

I

I

G

G

F

M

V

I

F

F

Q

Q

A

D

P

H

Y

H

L

L

I

L

P

M

F

D

L

R

D

T

V

V

T

Y

D

D

N

N

V

V

A

A

L

I

L

P

P

L

M

I

L

I

A

A

G

G

M

A

P

S

N

G

A

D

E

D

A

I

R

R

K

R

R

R

A

V

I

S

G

A

L

A

F

L

K

D

A

K

L

V

D

G

V

L

E

L

H

D

R

K

A

A

K

K

A

N

M

F

P

P

S

I

Q

Q

L

L

S

S

G

G

G

G

E

E

Q

Q

Q

Q

R

R

V

V

A

G

I

I

A

A

R

R

G

A

L

V

V

V

N

N

R

K

P

P

P

A

V

V

I

L

L

L

A

A

D

D

E

E

P

P

T

T

A

G

P

N

L

L

D

D

S

D

E

A

R

L

A

S

L

E

A

G

V

I

I

L

R

R

I

L

L

F

N

E

D

E

M

F

A

-

K

N

K

R

F

V

E

G

T

V

A

T

I

V

I

L

V

M

V

A

T

T

H

H

D

D

E

I

K

N

I

L

I

I

P

S

T

-

F

-

K

R

R

R

I

S

Y

Y

-

R

-

M

-

L

-

T

-

L

-

S

-

D

-

G

H

H

I

L

R

H

D

G

G

G

V

V

T

G

Y

H

E

E

K41 (= K48) mutation to R: Does not bind ATP.
C49 (≠ G56) mutation to A: Prevents dimer formation. Does not alter ATP-binding.

3c4jA Abc protein artp in complex with atp-gamma-s
38% identity, 86% coverage: 6:207/236 of query aligns to 4:197/242 of 3c4jA

query
sites
3c4jA

I

I

R

D

I

V

Q

H

G

Q

L

L

K

K

K

K

R

S

Y
x
F

G

G

S

S

G

-

D

-

T

-

A

-

V

L

D

E

A
x
V

L

L

K

K

T

G

V

I

D

N

M

V

H

H

V

I

A

R

P

E

G

G

E

E

V

V

V

V

G

V

L

V

I

I

G

G

P

P

S
|
S

G
|
G

S
|
S

G
|
G

K
|
K

T
x
S

T
|
T

L

F

L

L

K

R

C

C

L

L

G

N

A

L

V

L

I

E

E

D

P

F

T

D

A

E

G

G

K

E

M

I

I

I

L

I

G

-

D

-

D

-

V

-

I

-

Y

-

D

-

D

D

G

G

-

I

-

N

W

L

K

K

V

A

K

K

D

D

L

T

R

N

A

L

L

N

R

K

-

V

R

R

D

E

R

E

I

V

G

G

F

M

V

V

F

F

Q

Q

A

R

P

F

Y

N

L

L

I

F

P

P

F

H

L

M

D

T

V

V

T

L

D

N

N

N

V

I

A

T

L

L

L

A

P

P

M

M

-

K

L

V

A

R

G

K

M

W

P

P

N

R

A

E

E

K

A

A

R

E

K

A

R

K

A

A

I

M

G

E

L

L

F

L

K

D

A

K

L

V

D

G

V

L

E

K

H

D

R

K

A

A

K

H

A

A

M

Y

P

P

S

D

Q

S

L

L

S

S

G

G

G

G

E

Q

Q

A

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

G

A

L

L

V

A

N

M

R

E

P

P

P

K

V

I

I

M

L

L

A

F

D

D

E

E

P

P

T

T

A

S

P

A

L

L

D

D

S

P

E

E

R

M

A

V

L

G

A

E

V

V

I

L

R

S

I

V

L

M

N

K

D

Q

M

L

A

A

K

N

K

E

F

G

E

M

T

T

A

-

I

M

I

V

V

V

V

V

T

T

H

H

D

E

binding phosphothiophosphoric acid-adenylate ester: F13 (≠ Y15), V18 (≠ A24), S38 (= S44), G39 (= G45), S40 (= S46), G41 (= G47), K42 (= K48), S43 (≠ T49), T44 (= T50)

Query Sequence

>WP_005997848.1 NCBI__GCF_000143965.1:WP_005997848.1
MTAKGIRIQGLKKRYGSGDTAVDALKTVDMHVAPGEVVGLIGPSGSGKTTLLKCLGAVIE
PTAGKMILGDDVIYDDGWKVKDLRALRRDRIGFVFQAPYLIPFLDVTDNVALLPMLAGMP
NAEARKRAIGLFKALDVEHRAKAMPSQLSGGEQQRVAIARGLVNRPPVILADEPTAPLDS
ERALAVIRILNDMAKKFETAIIVVTHDEKIIPTFKRIYHIRDGVTYEEEGEGRGFE

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory