SitesBLAST
Comparing WP_006022528.1 NCBI__GCF_000015165.1:WP_006022528.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 19 hits to proteins with known functional sites (download)
P38502 Acetate kinase; Acetokinase; EC 2.7.2.1 from Methanosarcina thermophila (see 5 papers)
41% identity, 99% coverage: 4:394/396 of query aligns to 3:399/408 of P38502
- N7 (= N8) mutation to A: Almost abolishes catalytic activity. Requires increased magnesium levels for activity. Strongly decreases affinity for acetate.; mutation to D: Almost abolishes catalytic activity. Strongly decreases affinity for acetate.
- S10 (= S11) mutation S->A,T: Strongly decreases catalytic activity. Strongly decreases affinity for acetate.
- S12 (= S13) mutation to A: Decreases catalytic activity. Strongly decreases affinity for acetate. Requires increased magnesium levels for enzyme activity.; mutation to T: Decreases catalytic activity. Strongly decreases affinity for acetate.
- K14 (= K15) mutation to A: Strongly decreases enzyme activity.; mutation to R: Reduces enzyme activity.
- R91 (= R93) mutation R->A,L: Decreases catalytic activity. Decreases affinity for acetate.
- V93 (= V95) mutation to A: Decreases affinity for acetate.
- L122 (= L124) mutation to A: Decreases affinity for acetate.
- D148 (= D150) active site, Proton donor/acceptor; mutation D->A,E,N: Abolishes catalytic activity. Decreases affinity for acetate, but not for ATP.
- F179 (= F180) mutation to A: Decreases affinity for acetate.
- N211 (= N211) mutation to A: Slightly reduced enzyme activity.
- P232 (≠ A232) mutation to A: Decreases affinity for acetate.
- R241 (= R241) mutation R->K,L: Decreases catalytic activity. Strongly reduced affinity for ATP.
- E384 (= E379) mutation to A: Almost abolishes catalytic activity. Strongly decreases affinity for acetate. Requires strongly increased magnesium levels for enzyme activity.
1tuuA Acetate kinase crystallized with atpgs (see paper)
41% identity, 99% coverage: 4:394/396 of query aligns to 3:399/399 of 1tuuA
- active site: N7 (= N8), R91 (= R93), H180 (= H181), R241 (= R241), E384 (= E379)
- binding adenosine-5'-diphosphate: K14 (= K15), G210 (= G210), D283 (= D283), F284 (≠ M284), R285 (= R285), G331 (= G328), I332 (= I329), N335 (= N332)
- binding sulfate ion: R91 (= R93), H180 (= H181), G212 (= G212)
1tuuB Acetate kinase crystallized with atpgs (see paper)
41% identity, 98% coverage: 4:392/396 of query aligns to 3:397/398 of 1tuuB
- active site: N7 (= N8), R91 (= R93), H180 (= H181), R241 (= R241), E384 (= E379)
- binding adenosine monophosphate: D283 (= D283), R285 (= R285), G331 (= G328), I332 (= I329), N335 (= N332), S336 (≠ H333)
- binding trihydrogen thiodiphosphate: H180 (= H181), G212 (= G212), R241 (= R241)
7fj9A Kpacka (pduw) with amppnp complex structure
44% identity, 96% coverage: 4:384/396 of query aligns to 4:385/395 of 7fj9A
7fj8A Kpacka (pduw) with amp complex structure
44% identity, 96% coverage: 4:384/396 of query aligns to 4:385/395 of 7fj8A
4fwsA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with ctp (see paper)
42% identity, 98% coverage: 4:390/396 of query aligns to 4:389/394 of 4fwsA
- active site: N8 (= N8), R83 (= R93), H172 (= H181), R233 (= R241), E378 (= E379)
- binding cytidine-5'-triphosphate: G202 (= G210), N203 (= N211), G204 (= G212), D275 (= D283), L276 (≠ M284), R277 (= R285), G323 (= G328), I324 (= I329), N327 (= N332)
- binding 1,2-ethanediol: V21 (≠ G23), C24 (≠ N26), H115 (= H125), N203 (= N211), T232 (= T240), R233 (= R241), K262 (≠ R270)
4fwrA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with cmp (see paper)
42% identity, 98% coverage: 4:390/396 of query aligns to 4:389/394 of 4fwrA
- active site: N8 (= N8), R83 (= R93), H172 (= H181), R233 (= R241), E378 (= E379)
- binding cytidine-5'-monophosphate: G202 (= G210), N203 (= N211), D275 (= D283), L276 (≠ M284), R277 (= R285), G323 (= G328), I324 (= I329), N327 (= N332)
4fwqA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with gtp (see paper)
42% identity, 98% coverage: 4:390/396 of query aligns to 4:389/394 of 4fwqA
- active site: N8 (= N8), R83 (= R93), H172 (= H181), R233 (= R241), E378 (= E379)
- binding guanosine-5'-triphosphate: H172 (= H181), N203 (= N211), G204 (= G212), D275 (= D283), L276 (≠ M284), R277 (= R285), E280 (≠ S288), G323 (= G328), I324 (= I329), N327 (= N332)
4fwpA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with gdp (see paper)
42% identity, 98% coverage: 4:390/396 of query aligns to 4:389/394 of 4fwpA
- active site: N8 (= N8), R83 (= R93), H172 (= H181), R233 (= R241), E378 (= E379)
- binding 1,2-ethanediol: S11 (= S11), H115 (= H125), K262 (≠ R270)
- binding guanosine-5'-diphosphate: N203 (= N211), D275 (= D283), L276 (≠ M284), R277 (= R285), E280 (≠ S288), G323 (= G328), I324 (= I329), N327 (= N332), S328 (≠ H333)
4fwoA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with gmp (see paper)
42% identity, 98% coverage: 4:390/396 of query aligns to 4:389/394 of 4fwoA
- active site: N8 (= N8), R83 (= R93), H172 (= H181), R233 (= R241), E378 (= E379)
- binding guanosine-5'-monophosphate: G202 (= G210), N203 (= N211), D275 (= D283), L276 (≠ M284), R277 (= R285), E280 (≠ S288), G323 (= G328), I324 (= I329), N327 (= N332)
- binding 1,2-ethanediol: E100 (≠ A110), N104 (≠ A114)
4fwnA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with adenosine tetraphosphate (ap4) (see paper)
42% identity, 98% coverage: 4:390/396 of query aligns to 4:389/394 of 4fwnA
- active site: N8 (= N8), R83 (= R93), H172 (= H181), R233 (= R241), E378 (= E379)
- binding adenosine-5'-tetraphosphate: H172 (= H181), H200 (= H208), N203 (= N211), G204 (= G212), D275 (= D283), L276 (≠ M284), R277 (= R285), G323 (= G328), I324 (= I329), N327 (= N332)
4fwmA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with atp (see paper)
42% identity, 98% coverage: 4:390/396 of query aligns to 4:389/394 of 4fwmA
- active site: N8 (= N8), R83 (= R93), H172 (= H181), R233 (= R241), E378 (= E379)
- binding adenosine-5'-triphosphate: H172 (= H181), H200 (= H208), N203 (= N211), G204 (= G212), D275 (= D283), L276 (≠ M284), R277 (= R285), G323 (= G328), I324 (= I329), N327 (= N332)
- binding 1,2-ethanediol: H172 (= H181), R233 (= R241)
4fwkA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with amp (see paper)
42% identity, 98% coverage: 4:390/396 of query aligns to 4:389/394 of 4fwkA
- active site: N8 (= N8), R83 (= R93), H172 (= H181), R233 (= R241), E378 (= E379)
- binding adenosine monophosphate: G202 (= G210), N203 (= N211), D275 (= D283), L276 (≠ M284), R277 (= R285), G323 (= G328), I324 (= I329), N327 (= N332)
- binding 1,2-ethanediol: D103 (= D113), N104 (≠ A114), R107 (≠ K117)
2e1zA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with diadenosine tetraphosphate (ap4a) obtained after co- crystallization with atp (see paper)
42% identity, 98% coverage: 4:390/396 of query aligns to 4:389/394 of 2e1zA
- active site: N8 (= N8), R83 (= R93), H172 (= H181), R233 (= R241), E378 (= E379)
- binding bis(adenosine)-5'-tetraphosphate: N8 (= N8), R83 (= R93), H115 (= H125), G202 (= G210), N203 (= N211), G204 (= G212), P224 (≠ A232), R233 (= R241), D275 (= D283), L276 (≠ M284), R277 (= R285), G323 (= G328), I324 (= I329), N327 (= N332)
1x3nA Crystal structure of amppnp bound propionate kinase (tdcd) from salmonella typhimurium (see paper)
42% identity, 98% coverage: 4:390/396 of query aligns to 4:389/394 of 1x3nA
- active site: N8 (= N8), R83 (= R93), H172 (= H181), R233 (= R241), E378 (= E379)
- binding phosphoaminophosphonic acid-adenylate ester: G202 (= G210), N203 (= N211), G204 (= G212), D275 (= D283), L276 (≠ M284), R277 (= R285), G323 (= G328), I324 (= I329), N327 (= N332)
1x3mA Crystal structure of adp bound propionate kinase (tdcd) from salmonella typhimurium (see paper)
42% identity, 98% coverage: 4:390/396 of query aligns to 4:389/394 of 1x3mA
- active site: N8 (= N8), R83 (= R93), H172 (= H181), R233 (= R241), E378 (= E379)
- binding adenosine-5'-diphosphate: G202 (= G210), N203 (= N211), D275 (= D283), L276 (≠ M284), R277 (= R285), G322 (≠ A327), G323 (= G328), I324 (= I329), N327 (= N332)
4iz9A Crystal structure of an acetate kinase from mycobacterium avium bound to an unknown acid-apcpp conjugate and manganese (see paper)
39% identity, 98% coverage: 2:391/396 of query aligns to 4:379/381 of 4iz9A
- active site: N10 (= N8), R74 (= R93), H163 (= H181), R224 (= R241), E367 (= E379)
- binding diphosphomethylphosphonic acid adenosyl ester: K17 (= K15), G193 (= G210), N194 (= N211), D265 (≠ S282), F266 (vs. gap), R267 (vs. gap), G313 (= G328), I314 (= I329), N317 (= N332), D318 (≠ H333)
4ijnA Crystal structure of an acetate kinase from mycobacterium smegmatis bound to amp and sulfate (see paper)
41% identity, 97% coverage: 1:384/396 of query aligns to 1:370/376 of 4ijnA
- active site: N8 (= N8), R72 (= R93), H161 (= H181), R222 (= R241), E365 (= E379)
- binding adenosine monophosphate: G191 (= G210), N192 (= N211), D263 (= D283), F264 (≠ M284), R265 (= R285), G311 (= G328), V312 (≠ I329), N315 (= N332), V316 (≠ H333)
1sazA Membership in the askha superfamily: enzymological properties and crystal structure of butyrate kinase 2 from thermotoga maritima (see paper)
24% identity, 56% coverage: 171:392/396 of query aligns to 144:347/375 of 1sazA
- active site: R214 (= R241)
- binding phosphomethylphosphonic acid adenylate ester: H154 (= H181), G184 (= G210), G185 (≠ N211), G186 (= G212), S254 (= S282), D255 (= D283), A256 (≠ M284), R257 (= R285), G304 (= G328), L305 (≠ I329), H307 (≠ E331)
Query Sequence
>WP_006022528.1 NCBI__GCF_000015165.1:WP_006022528.1
MKAIFSLNSGSSSIKFALFTLDGANNPMLSAGGKIERIGIAPSLRVRSADGDIVLERDWP
DGASLSHAELLKDIFAWAAERLGDRQVIAIGHRVVHGGTEFAAPRLVDDAVLDALEKLNP
LAPLHQPHNLAAIRAIAQLRPGLPQVACFDTAFHHDKPPVAARLPLPRAFHEQGIRRYGF
HGLSYEYIARRLREIDPVLAAGRMIAAHLGNGASLCAMRDGKSIDTTMGFTALDGLMMGT
RCGAIDPGVVLHLQTQLGMSAADVEDLLYRKSGLLGVSGISSDMRTLSDNSGPEAEEAIE
LFCWRVAREAGGLIASLGGLDAFVFTAGIGENHADVRRRICERLAWSGLWIDESANLGSA
LRICEANSPVAVLVIPTNEERMIALHTLNVVREDHS
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory