SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_006746648.1 NCBI__GCF_000227685.2:WP_006746648.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 9 hits to proteins with known functional sites (download)

P08243 Asparagine synthetase [glutamine-hydrolyzing]; Cell cycle control protein TS11; Glutamine-dependent asparagine synthetase; EC 6.3.5.4 from Homo sapiens (Human) (see 7 papers)
29% identity, 58% coverage: 1:392/672 of query aligns to 1:379/561 of P08243

query
sites
P08243

M

M

C
|
C

G

G

I

I

A

W

G
x
A

I

L

F

F

H

G

A

S

D

D

P

-

E

-

R

-

V

-

D

D

P

C

E

L

T

S

L

V

V

Q

A

C

M

L

A

S

A

A

I

M

Q

K

Y

I

-

A

H

H

R

R

G

G

P

P

D

D

G

A

F

F

G

R

Y

F

-

E

R

N

V

V

Q

N

Q

G

D

Y

R

T

G

N

V

C

G

C

F

F

S

G

H

F

A

H

R

R

L

L

S

A

I

V

D

D

L

-

D

-

E

P

D

L

R

F

G

G

R

M

Q

Q

P

P

F

I

-

R

V

V

M

K

P

K

D

Y

N

P

S

Y

I

L

M

W

T

L

A

C

V

Y

N

N

G

G

E

E

L

I

Y

Y

D

N

Y

H

K

K

R

K

I

M

R

Q

A

Q

D

H

L

F

T

E

L

F

R

-

G

-

V

-

K

E

F

Y

R

Q

T

T

K

K

S

V

D

D

S

G

E

E

M

I

V

I

M

L

H

H

L

L

Y

Y

R

D

R

K

V

G

G

G

L

I

E

E

D

Q

A

T

I

I

P

C

H

M

F

L

R

D

G

G

E

V

F

F

A

A

I

F

S

V

L

L

Y

L

D

D

R

T

E

A

H

N

D

K

R

K

L

V

V

F

L

L

I

G

R

R

D

D

R

T

F

Y

G

G

V

V

K

R

P

P

L

L

Y

F

-

K

-

A

F

M

T

T

E

E

A

-

N

D

G

G

A

F

F

L

V

A

F

V

G

C

S

S

E

E

L

A

K

K

V

G

L

L

F

V

A

-

H

-

P

-

D

-

V

-

P

-

R

-

F

-

S

-

D

-

T

-

G

-

L

-

F

-

H

-

Q

-

L

-

M

-

Q

-

T

T

I

L

V

K

P

H

G

S

T

A

T

T

A

P

F

F

E

L

G

K

V

V

H

E

Q

P

V

F

K

L

P

P

G

G

H

H

M

Y

V

E

I

V

V

L

E

D

-

L

R

K

A

P

H

N

G

G

K

K

L

V

-

A

K

S

V

V

R

E

Q

M

E

V

K

K

Y

Y

W

H

D

H

M

C

-

R

D

D

F
x
V

P

P

L

L

A

H

A

A

E

L

R

Y

P

D

E

N

H

V

D

E

D

K

T

L

D

F

E

P

E

G

Y

F

W

E

I

I

E

E

G

T

V

V

R

K

E

N

H

N

L

L

-

R

-

I

-

L

-

F

M

N

E

N

A

A

V

V

Q

K

L

K

R

R

L

L

E

M

A

T

D

D

V

R

P

R

V

I

A

G

C

C

Y

L

L

L

S

S

G

G

I

L

D

D

S

S

C

S

I

L

T

V

L

A

G

A

-

T

-

L

-

K

-

Q

L

L

A

K

A

E

A

A

S

Q

Q

V

Q

Q

S

Y

P

P

V

L

K

Q

A

T

F

F

T

A

I

I

G

G

F

M

D

E

D

D

A

S

A

P

Y

-

D

-

E

D

T

L

A

L

I

A

A

A

R

R

E

K

M

V

A

A

E

D

S

H

V

I

G

G

A

S

D

E

Q

H

D

Y

I

E

M

V

T

L

L

F

Q

N

A

S

D

E

H

E

L

G

Y

I

D

Q

N

A

L

L

V

D

E

E

T

V

L

I

W

F

H

S

T

L

E

E

R

T

S

Y

I

D

Y

I

N

T

T

T

L

V

-

R

-

A

G

S

V

V

A

G

K

M

L

Y

L

L

M

I

S

S

R

K

H

Y

V

I

N

R

E

K

A

N

G

T

Y

D

K

S

V

V

V

I

-
x
F

T

S

G

G

E

E

G

G

S

S

D

D

E

E

L

L

F

T

A

Q

G

G

Y

Y

P

I

A

Y

F

F

R

H

R

K

C2 (= C2) active site, For GATase activity; mutation to A: Loss of the glutamine-dependent asparagine synthetase activity, while the ammonia-dependent activity remained unaffected.
A6 (≠ G6) to E: in ASNSD; dramatic reduction in protein abundance; dbSNP:rs398122975
V210 (≠ F233) to E: in dbSNP:rs1049674
F362 (vs. gap) to V: in ASNSD; dramatic reduction in protein abundance; dbSNP:rs398122973

Sites not aligning to the query:

550 R → C: in ASNSD; increases level of protein abundance; dbSNP:rs398122974

P78753 Probable asparagine synthetase [glutamine-hydrolyzing]; Glutamine-dependent asparagine synthetase; EC 6.3.5.4 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
28% identity, 58% coverage: 1:390/672 of query aligns to 1:368/557 of P78753

query
sites
P78753

M

M

C

C

G

G

I

I

A

L

G

A

I

V

F

H

H

H

A

V

D

A

P

E

E

D

R

I

R

E

V

A

D

F

P

K

E

P

T

K

L

A

V

L

A

H

M

L

A

S

A

K

I

Q

Q

L

Y

R

H

H

R

R

G

G

P

P

D

D

G

W

F

S

G

G

Y

K

R

A

V

I

Q

R

Q

N

D

Q

R

-

G

-

V

T

G

I

F

L

S

C

H

H

A

E

R

R

L

L

S

A

I

I

I

V

D

G

L

V

D

E

E

S

D

-

R

-

G

G

R

A

Q

Q

P

P

F

L

V

V

M

S

P

D

D

D

N

G

S

K

I

L

M

V

T

L

A

T

V

V

N

N

G

G

E

E

L

I

Y

Y

D

N

Y

H

K

L

R

K

I

L

R

R

A

E

D

N

L

L

T

K

L

-

R

G

G

N

V

Y

K

K

F

F

R

K

T

T

K

Y

S

S

D

D

S

C

E

E

M

V

V

I

M

L

H

Y

L

L

Y

Y

R

R

R

E

V

H

G

G

L

P

E

A

D

C

A

A

I

-

P

N

H

M

F

L

R

D

G

G

E

M

F

F

A

S

I

W

S

V

L

L

Y

Y

D

D

R

Q

E

D

H

K

D

D

R

K

L

V

V

V

L

A

I

A

R

R

D

D

R

P

F

I

G

G

V

I

K

T

P

T

L

L

Y

Y

-

Q

-

G

-

F

F

S

T

S

E

D

A

S

N

P

G

D

A

T

F

A

V

Y

F

F

G

A

S

S

E

E

L

L

K

K

V

A

L

L

F

-

A

-

H

H

P

P

D

-

V

-

P

-

R

-

F

-

S

-

D

-

T

-

G

-

L

-

F

-

H

-

Q

-

L

-

M

-

Q

-

T

-

I

V

V

C

P

D

G

K

T

I

A

A

F

F

E

-

G

-

V

-

H

-

Q

-

V

-

K

P

P

P

G

G

H

H

M

Y

V

Y

I

D

V

S

E

E

R

T

A

K

H

Q

G

-

K

T

L

V

K

R

V

Y

R

F

Q

K

E

P

K

S

Y

W

W

W

D

D

M

E

D

N

F

-

P

-

L

-

A

-

E

-

R

K

P

I

E

P

H

S

D

N

D

P

T

V

D

D

E

Y

E

K

Y

L

W

-

I

-

E

-

G

-

V

L

R

R

E

E

H

T

L

L

M

E

E

A

A

S

V

V

Q

R

L

K

R

R

L

L

E

M

A

A

D

E

V

V

P

P

V

Y

A

G

C

V

Y

L

L

L

S

S

G

G

I

L

D

D

S

S

C

S

I

L

-

I

-

A

-

S

-

I

-

A

-

R

-

E

T

T

L

E

G

K

L

L

A

A

A

N

A

S

S

T

Q

S

Q

Q

S

S

-

E

-

A

-

R

-

T

-

I

-

T

-

A

-

W

-

P

P

K

V

L

K

H

A

S

F

F

T

A

I

I

G

G

F

L

D

P

D

G

A

S

A

P

Y

-

D

-

E

D

T

L

A

L

I

A

A

A

R

R

E

K

M

V

A

A

E

D

S

F

V

L

G

H

A

T

D

F

Q

H

D

H

I

E

M

H

T

T

L

F

Q

T

A

I

D

D

H

E

L

G

Y

L

D

D

N

A

L

L

V

R

E

D

T

V

L

I

W

Y

H

H

T

L

E

E

R

T

S

Y

I

D

Y

V

N

T

T

T

L

I

G

R

V

A

A

S

K

T

-

P

-

M

L

Y

L

L

M

L

S

S

R

R

H

K

V

I

N

K

E

A

A

Q

G

G

Y

V

K

K

V

M

V

V

V

L

T

S

G

G

E

E

G

G

S

S

D

D

E

E

L

I

F

F

A

G

G

G

Y

Y

P

L

A

Y

F

F

Sites not aligning to the query:

391 modified: Phosphoserine
489 modified: Phosphoserine

1ct9A Crystal structure of asparagine synthetase b from escherichia coli (see paper)
27% identity, 58% coverage: 3:392/672 of query aligns to 2:346/497 of 1ct9A

query
sites
1ct9A

G

S

I

I

A

F

G

G

I

V

F

F

H

D

A

I

D

K

P

T

E

D

R

A

R

V

V

E

D

L

P

R

E

K

T

K

L

A

V

L

A

E

M

L

A

S

A

R

I

L

Q

M

Y

R

H

H

R

R

G

G

P

P

D

D

G

W

F

S

G

G

Y

-

R

-

V

I

Q

Y

Q

A

D

S

R

D

G

N

V

A

G

I

F

L

S

A

H

H

A

E

R
|
R

L
|
L

S

S

I
|
I

I
x
V

D

D

L

V

D

N

E

A

D

-

R

-

G

G

R

A

Q

Q

P

P

F

L

V

Y

M

N

P

Q

D

Q

N

K

S

T

I

H

M

V

T

L

A

A

V

V

N
|
N

G
|
G

E
|
E

L

I

Y

Y

D

N

Y

H

K

Q

R

A

I

L

R

R

A

A

D

E

L

Y

T

G

L

D

R

R

G

-

V

Y

K

Q

F

F

R

Q

T

T

K

G

S

S

D
|
D

S

C

E

E

M

V

V

I

M

L

H

A

L

L

Y

Y

R

Q

R

E

V

K

G

G

L

P

E

E

D

-

A

F

I

L

P

D

H

D

F

L

R

Q

G

G

E

M

F

F

A

A

I

F

S

A

L

L

Y

Y

D

D

R

S

E

E

H

K

D

D

R

A

L

Y

V

L

L

I

I

G

R

R

D

D

R

H

F

L

G

G

V

I

K

I

P

P

L

L

Y

Y

F

M

-

G

T

Y

E

D

A

E

N

H

G

G

A

Q

F

L

V

Y

F

V

G

A

S

S

E

E

L

M

K

K

V

A

L

L

F

V

A

P

H

V

P

C

D

R

V

T

P

I

R

K

R

E

F

F

-

P

S

A

D

G

T

S

G

Y

L

L

F

W

H

S

Q

Q

L

-

M

-

Q

-

T

-

I

-

V

-

P

-

G

-

T

-

A

-

F

-

E

-

G

-

V

-

H

-

Q

-

V

-

K

-

P

-

G

-

H

-

M

-

V

-

I

-

V

-

E

-

R

-

A

-

H

D

G

G

K

E

L

I

K

R

V

S

R

Y

Q

Y

E

H

K

R

Y

D

W

W

D

-

M

-

D

-

F

F

P

D

L

Y

A

D

A

A

E

V

R

K

P

D

E

N

H

V

D

T

D

D

T

K

D

N

E

E

E

-

Y

-

W

-

I

-

E

-

G

-

V

L

R

R

E

Q

H

A

L

L

M

E

E

D

A

S

V

V

Q

K

L

S

R

H

L

L

E

M

A

S

D

D

V

V

P

P

V

Y

A

G

C

V

Y
x
L

L
|
L

S
|
S

G

G

I

L

D

D

S
|
S

C

S

I

I

T

-

L

-

G

-

L

I

A

S

A

A

A

I

S

T

Q

K

S

Y

P

A

V

L

K

H

A

S

F

F

T
x
A

I
x
V

G

G

F

L

D

P

D

G

A

S

A

P

Y

-

D

-

E
x
D

T

L

A

K

I

A

A

A

R

Q

E

E

M

V

A

A

E

N

S

H

V

L

G

G

A

T

D

V

Q

H

D

H

I

E

M

I

T

H

L

F

Q

T

A

V

D

Q

H

E

L

G

Y

L

D

D

N

A

L

I

V

R

E

D

T

V

L

I

W

Y

H

H

T

I

E

E

R

T

S

Y

I

D

Y

V

N
x
T

T

T

L

I

G
x
R

V

A

A

S

K

T

-

P

-

M

L

Y

L

L

M
|
M

S

S

R

R

H

K

V

I

N

K

E

A

A

M

G

G

Y

I

K

K

V

M

V

V

V

L

T
x
S

G
|
G

E
|
E

G

G

S

S

D

D

E

E

L

V

F

F

A

G

G

G

Y

Y

P

L

A

Y

F

F

R

H

R

K

active site: L50 (= L53), N74 (= N79), G75 (= G80), T305 (≠ N353), R308 (≠ G356), E332 (= E378)
binding adenosine monophosphate: L232 (≠ Y275), L233 (= L276), S234 (= S277), S239 (= S282), A255 (≠ T301), V256 (≠ I302), D263 (≠ E311), M316 (= M362), S330 (≠ T376), G331 (= G377), E332 (= E378)
binding glutamine: R49 (= R52), L50 (= L53), I52 (= I55), V53 (≠ I56), N74 (= N79), G75 (= G80), E76 (= E81), D98 (= D104)

Sites not aligning to the query:

active site: 1, 366
binding glutamine: 1

P22106 Asparagine synthetase B [glutamine-hydrolyzing]; AS-B; EC 6.3.5.4 from Escherichia coli (strain K12) (see 2 papers)
26% identity, 58% coverage: 1:392/672 of query aligns to 1:363/554 of P22106

query
sites
P22106

M
|
M

C
|
C

G

S

I

I

A

F

G

G

I

V

F

F

H

D

A

I

D

K

P

T

E

D

R

A

R

V

V

E

D

L

P

R

E

K

T

K

L

A

V

L

A

E

M

L

A

S

A

R

I

L

Q

M

Y

R

H
|
H

R

R

G

G

P

P

D
|
D

G

W

F

S

G

G

Y

-

R

-

V

I

Q

Y

Q

A

D

S

R

D

G

N

V

A

G

I

F

L

S

A

H

H

A

E

R

R

L

L

S

S

I

I

I

V

D

D

L

V

D

N

E

A

D

-

R

-

G

G

R

A

Q

Q

P

P

F

L

V

Y

M

N

P

Q

D

Q

N

K

S

T

I

H

M

V

T

L

A

A

V

V

N

N

G

G

E

E

L

I

Y

Y

D

N

Y
x
H

K

Q

R

A

I

L

R

R

A

A

D

E

L

Y

T

G

L

D

R

R

G

-

V

Y

K

Q

F

F

R

Q

T

T

K

G

S

S

D

D

S

C

E

E

M

V

V

I

M

L

H
x
A

L

L

Y

Y

R

Q

R

E

V

K

G

G

L

P

E

E

D

-

A

F

I

L

P

D

H

D

F

L

R

Q

G

G

E

M

F

F

A

A

I

F

S

A

L

L

Y

Y

D

D

R

S

E

E

H

K

D

D

R

A

L

Y

V

L

L

I

I

G

R

R

D

D

R

H

F

L

G

G

V

I

K

I

P

P

L

L

Y

Y

F

M

-

G

T

Y

E

D

A

E

N

H

G

G

A

Q

F

L

V

Y

F

V

G

A

S

S

E

E

L

M

K

K

V

A

L

L

F

V

A

P

H

V

P

C

D

R

V

T

P

I

R

K

R

E

F

F

-

P

S

A

D

G

T

S

G

Y

L

L

F

W

H

S

Q

Q

L

-

M

-

Q

-

T

-

I

-

V

-

P

-

G

-

T

-

A

-

F

-

E

-

G

-

V

-

H

-

Q

-

V

-

K

-

P

-

G

-

H

-

M

-

V

-

I

-

V

-

E

-

R

-

A

-

H

D

G

G

K

E

L

I

K

R

V

S

R

Y

Q

Y

E

H

K

R

Y

D

W

W

D

-

M

-

D

-

F

F

P

D

L

Y

A

D

A

A

E

V

R

K

P

D

E

N

H

V

D

T

D

D

T

K

D

N

E

E

E

-

Y

-

W

-

I

-

E

-

G

-

V

L

R

R

E

Q

H

A

L

L

M

E

E

D

A

S

V

V

Q

K

L

S

R

H

L

L

E

M

A

S

D

D

V

V

P

P

V

Y

A

G

C

V

Y

L

L

L

S

S

G

G

I

L

D

D

S

S

C

S

I

I

T

I

L

S

G

A

L

I

A

T

A

K

S

Y

Q

A

S

R

P

R

V

V

K

E

-

D

-

Q

-

E

-

R

-

S

-

E

-

A

-

W

-

P

-

Q

-

L

-

H

A

S

F

F

T

A

I

V

G

G

F

L

D

P

D

G

A

S

A

P

Y

-

D

-

E

D

T

L

A

K

I

A

A

A

R

Q

E

E

M

V

A

A

E

N

S

H

V

L

G

G

A

T

D

V

Q

H

D

H

I

E

M

I

T

H

L

F

Q

T

A

V

D

Q

H

E

L

G

Y

L

D

D

N

A

L

I

V

R

E

D

T

V

L

I

W

Y

H

H

T

I

E

E

R

T

S

Y

I

D

Y

V

N

T

T

T

L

I

G

R

V

A

A

S

K

T

-

P

-

M

L

Y

L

L

M

M

S

S

R

R

H

K

V

I

N

K

E

A

A

M

G

G

Y

I

K

K

V

M

V

V

V

L

T

S

G

G

E
|
E

G

G

S

S

D

D

E

E

L

V

F

F

A

G

G

G

Y

Y

P

L

A

Y

F

F

R

H

R

K

M1 (= M1) modified: Initiator methionine, Removed
C2 (= C2) mutation C->A,S: Loss of glutamine-dependent activity but no effect on ammonia-dependent asparagine synthetase activity.
H30 (= H30) mutation to A: 4,5-fold decrease in glutamine affinity.
D34 (= D34) mutation D->N,E: Little effect on the kinetic properties.
H81 (≠ Y85) mutation to A: 5-fold decrease in glutamine affinity.
A105 (≠ H110) mutation to H: Little effect on the kinetic properties.
E349 (= E378) mutation E->A,Q: Loss of glutamine- and ammonia-dependent synthetase activity, but still exhibits glutaminase activity.; mutation to D: 5-fold increase in affinity for aspartate when assaying both the glutamine- and ammonia-dependent synthetase reactions, and 2-fold decrease in kcat for these reactions. Modifies the product glutamate/asparagine stoichiometry.

6gq3A Human asparagine synthetase (asns) in complex with 6-diazo-5-oxo-l- norleucine (don) at 1.85 a resolution (see paper)
28% identity, 58% coverage: 2:392/672 of query aligns to 1:366/509 of 6gq3A

query
sites
6gq3A

C
|
C

G

G

I

I

A
x
W

G

A

I

L

F

F

H

G

A

S

D

D

P

-

E

-

R

-

V

-

D

D

P

C

E

L

T

S

L

V

V

Q

A

C

M

L

A

S

A

A

I

M

Q

K

Y

I

-

A

H

H

R

R

G

G

P

P

D

D

G

A

F

F

G

R

Y

F

-

E

R

N

V

V

Q

N

Q

G

D

Y

R

T

G

N

V

C

G

C

F

F

S

G

H

F

A

H

R
|
R

L
|
L

S

A

I
x
V

D

D

L

-

D

-

E

P

D

L

R

F

G

G

R

M

Q

Q

P

P

F

I

-

R

V

V

M

K

P

K

D

Y

N

P

S

Y

I

L

M

W

T

L

A

C

V
x
Y

N
|
N

G
|
G

E
|
E

L

I

Y

Y

D

N

Y

H

K

K

R

K

I

M

R

Q

A

Q

D

H

L

F

T

E

L

F

R

-

G

-

V

-

K

E

F

Y

R

Q

T

T

K

K

S
x
V

D
|
D

S

G

E

E

M

I

V

I

M

L

H

H

L

L

Y

Y

R

D

R

K

V

G

G

G

L

I

E

E

D

Q

A

T

I

I

P

C

H

M

F

L

R

D

G

G

E

V

F

F

A

A

I

F

S

V

L

L

Y

L

D

D

R

T

E

A

H

N

D

K

R

K

L

V

V

F

L

L

I

G

R

R

D

D

R

T

F

Y

G

G

V

V

K

R

P

P

L

L

Y

F

-

K

-

A

F

M

T

T

E

E

A

-

N

D

G

G

A

F

F

L

V

A

F

V

G

C

S

S

E

E

L

A

K

K

V

G

L

L

F

V

A

-

H

-

P

-

D

-

V

-

P

-

R

-

F

-

S

-

D

-

T

-

G

-

L

-

F

-

H

-

Q

-

L

-

M

-

Q

-

T

T

I

L

V

K

P

H

G

S

T

A

T

T

A

P

F

F

E

L

G

K

V

V

H

E

Q

P

V

F

K

L

P

P

G

G

H

H

M

Y

V

E

I

V

V

L

E

D

-

L

R

K

A

P

H

N

G

G

K

K

L

V

-

A

K

S

V

V

R

E

Q

M

E

V

K

K

Y

Y

W

H

D

H

M

C

D

-

F

-

P

-

L

-

A

-

E

-

R

-

P

-

E

-

H

-

D

R

D

D

T

V

D

F

E

P

E

G

Y

F

W

E

I

I

E

E

G

T

V

V

R

K

E

N

H

N

L

L

M

R

-

I

-

L

-

F

-

N

E

N

A

A

V

V

Q

K

L

K

R

R

L

L

E

M

A

T

D

D

V

R

P

R

V

I

A

G

C

C

Y

L

L

L

S

S

G

G

I

L

D

D

S

S

C

S

I

L

T

V

L

A

G

A

-

T

-

L

-

K

-

Q

L

L

A

K

A

E

A

A

S

Q

Q

V

Q

Q

S

Y

P

P

V

L

K

Q

A

T

F

F

T

A

I

I

G

G

F

M

D

E

D

D

A

S

A

P

Y

-

D

-

E

D

T

L

A

L

I

A

A

A

R

R

E

K

M

V

A

A

E

D

S

H

V

I

G

G

A

S

D

E

Q

H

D

Y

I

E

M

V

T

L

L

F

Q

N

A

S

D

E

H

E

L

G

Y

I

D

Q

N

A

L

L

V

D

E

E

T

V

L

I

W

F

H

S

T

L

E

E

R

T

S

Y

I

D

Y

I

N
x
T

T

T

L

V

-
x
R

-

A

G

S

V

V

A

G

K

M

L

Y

L

L

M

I

S

S

R

K

H

Y

V

I

N

R

E

K

A

N

G

T

Y

D

K

S

V

V

V

I

-

F

T

S

G

G

E

E

G

G

S

S

D

D

E

E

L

L

F

T

A

Q

G

G

Y

Y

P

I

A

Y

F

F

R

H

R

K

active site: W4 (≠ A5), L49 (= L53), N74 (= N79), G75 (= G80), T324 (≠ N353), R327 (vs. gap)
binding 5-oxo-l-norleucine: C1 (= C2), R48 (= R52), V51 (≠ I55), V52 (≠ I56), Y73 (≠ V78), N74 (= N79), G75 (= G80), E76 (= E81), V95 (≠ S103), D96 (= D104)

Q9STG9 Amidophosphoribosyltransferase 2, chloroplastic; AtATase2; AtPURF2; PRPP2; Glutamine phosphoribosylpyrophosphate amidotransferase 2; AtGPRAT2; Protein CHLOROPLAST IMPORT APPARATUS 1; Protein DIFFERENTIAL DEVELOPMENT OF VASCULAR ASSOCIATED CELLS; EC 2.4.2.14 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
32% identity, 25% coverage: 2:170/672 of query aligns to 87:285/561 of Q9STG9

query
sites
Q9STG9

C

C

G

G

I

V

A

V

G

G

I

I

F

Y

H

G

A

D

D

S

P

E

E

A

R

S

R

R

V

L

D

-

P

-

E

-

T

C

L

Y

V

L

A

A

M

L

A

H

A

A

I

L

Q

Q

Y

-

H

H

R

R

G

G

P

Q

D

E

G

G

F

A

G

G

Y

I

-

V

R

T

V

V

Q

S

Q

K

D

D

R

K

-

V

-

L

-

Q

-

T

-

I

-

T

G

G

V

V

G

G

F

L

-

V

-

S

-

E

-

V

-

F

-

S

-

E

-

S

-

K

-

L

-

D

-

Q

-

L

-

P

-

G

-

D

-

I

-

A

-

I

S

G

H

H

A

V

R

R

L

Y

S

S

I

T

I

A

D

G

L

S

D

S

E

M

D

L

R

K

G

N

R

V

Q

Q

P

P

F

F

V

V

M

A

P

G

D

Y

-

R

-

F

N

G

S

S

I

V

M

G

T

V

A

A

V
x
H

N

N

G

G

E

N

L

L

Y

V

D

N

Y

Y

K

T

R

K

I

L

R

R

A

A

D

D

L

L

T

E

L

E

R

N

G

G

V

S

K

I

F

F

R

N

T

T

K

S

S

S

D

D

S

T

E

E

M

V

V

V

M

L

H

H

L

L

Y

-

R

-

V

I

G

A

L

I

E

S

D

K

A

A

I

R

P

P

H

F

F

F

-

M

-

R

-

I

-

V

-

D

-

A

-

C

-

E

R

K

G

L

E

Q

F

G

A

A

I

Y

S

S

L

M

Y

V

D

F

R

V

E

T

H

E

D

D

R

K

L

L

V

V

L

A

I

V

R

R

D

D

R

P

F

H

G

G

V

F

K
x
R

P
|
P

L

L

Y

V

F

M

-

G

T

R

E

R

A

S

N

N

G

G

A

A

F

V

V

V

F

F

G

A

S

S

E

E

L

T

K

C

V

A

L

L

H187 (≠ V78) mutation to T: In cia1-2; small plants with white leaves showing an irregular mosaic of green sectors.
R264 (≠ K150) mutation to K: Strong resistance to the bleaching herbicides DAS073 and DAS734.
P265 (= P151) mutation to S: Low resistance to the bleaching herbicides DAS073 and DAS734; when associated with F-494.

Sites not aligning to the query:

371 G→S: Low resistance to the bleaching herbicides DAS073 and DAS734.
476 P→S: Resistance to the bleaching herbicides DAS073 and DAS734.
494 Y→F: Low resistance to the bleaching herbicides DAS073 and DAS734; when associated with S-265.

6lbpA Structure of the glutamine phosphoribosylpyrophosphate amidotransferase from arabidopsis thaliana (see paper)
32% identity, 25% coverage: 2:170/672 of query aligns to 1:199/460 of 6lbpA

query
sites
6lbpA

C
|
C

G

G

I

V

A

V

G

G

I

I

F

Y

H

G

A

D

D

S

P

E

E

A

R

S

R

R

V

L

D

-

P

-

E

-

T

C

L

Y

V

L

A

A

M

L

A

H

A

A

I

L

Q

Q

Y

-

H

H

R

R

G
|
G

P

Q

D

E

G

G

F

A

G

G

-

I

Y

V

R

T

V

V

Q

S

Q

K

D

D

R

K

-

V

-

L

-

Q

-

T

-

I

-

T

G

G

V

V

G

G

F

L

-

V

-

S

-

E

-

V

-

F

-

S

-

E

-

S

-

K

-

L

-

D

-

Q

-

L

-

P

-

G

-

D

-

I

-

A

-

I

S

G

H

H

A

V

R

R

L

Y

S

S

I

T

I

A

D

G

L

S

D

S

E

M

D

L

R

K

G

N

R

V

Q

Q

P

P

F

F

V

V

M

A

P

G

D

Y

-

R

-

F

N

G

S

S

I

V

M

G

T

V

A

A

V

H

N
|
N

G
|
G

E

N

L

L

Y

V

D

N

Y

Y

K

T

R

K

I

L

R

R

A

A

D

D

L

L

T

E

L

E

R

N

G

G

V

S

K

I

F

F

R

N

T

T

K

S

S

S

D

D

S

T

E

E

M

V

V

V

M

L

H

H

L

L

Y

-

R

-

V

I

G

A

L

I

E

S

D

K

A

A

I

R

P

P

H

F

F

F

-

M

-

R

-

I

-

V

-

D

-

A

-

C

-

E

R

K

G

L

E

Q

F

G

A

A

I

Y

S

S

L

M

Y

V

D

F

R

V

E

T

H

E

D

D

R

K

L

L

V

V

L

A

I

V

R

R

D

D

R

P

F

H

G

G

V

F

K

R

P

P

L

L

Y

V

F

M

-

G

T

R

E

R

A

S

N

N

G

G

A

A

F

V

V

V

F

F

G

A

S

S

E

E

L

T

K

C

V

A

L

L

active site: C1 (= C2), G27 (= G32), N102 (= N79), G103 (= G80)

Sites not aligning to the query:

active site: 243, 301, 306, 316, 424
binding iron/sulfur cluster: 237, 239, 383, 385, 434, 436, 437

1jgtB Crystal structure of beta-lactam synthetase (see paper)
26% identity, 57% coverage: 33:413/672 of query aligns to 6:377/500 of 1jgtB

query
sites
1jgtB

P

P

D

A

G

A

F

F

G

G

Y

F

R

L

V

A

Q

S

Q

A

D

R

R

T

G

G

V

G

G

G

-

P

-

G

-

P

F

V

S

F

H

A

A

T

R

R

L

G

S

S

I

H

I

T

D

D

L

I

D

D

E

T

D

P

R

Q

G

G

R

E

Q

R

-

S

-

L

-

A

-

T

-

L

-

V

-

H

-

A

P

P

F

S

V

V

M

A

P

P

D

D

N

R

S

A

I

V

-

A

-

R

-

S

-

L

-

T

-

G

-

A

-

P

M

T

T

T

A

A

V

V

-

L

N
x
A

G
|
G

E

E

L

I

Y

Y

D

N

Y

R

K

D

R

E

I

L

R

-

A

-

D

-

L

L

T

S

L

V

R

L

G

P

V

A

K

G

F

P

R

A

T

P

K

E

S

G

D

D

S

A

E

E

M

L

V

V

M

L

H

R

L

L

Y

L

R

E

R

R

V

Y

G

D

L

L

E

H

D

-

A

A

I

F

P

R

H

L

F

V

R

N

G

G

E

R

F

F

A

A

I

T

S

V

L

V

Y

-

D

-

R

R

E

T

H

G

D

D

R

R

L

V

V

L

L

L

I

A

R

T

D

D

R

H

F

A

G

G

V

S

K

V

P

P

L

L

Y

Y

F

T

T

C

E

V

A

A

N

P

G

G

A

E

F

V

V

R

F

A

G

S

S

T

E

E

L

A

K

K

V

A

L

L

F

A

A

A

H

H

P

R

D

D

V

-

P

P

R

K

R

G

F

F

S

P

D

L

T

A

G

D

L

A

F

R

H

R

Q

-

L

-

M

-

Q

-

T

-

I

-

V

V

P

A

G

G

T

L

T

T

A

-

F

-

E

-

G

G

V

V

H

Y

Q

Q

V

V

K

P

P

A

G

G

H

A

M

V

V

M

I

D

V

I

E

D

R

L

A

G

H

S

G

G

K

-

L

-

K

T

V

A

R

V

Q

T

E

H

K

R

Y

T

W

W

D

T

M

-

D

-

F

-

P

P

L

G

A

L

A

S

E

R

R

R

-

I

-

L

P

P

E

E

H

G

D

E

D

-

T

-

D

-

E

-

Y

-

W

A

I

V

E

A

G

A

V

V

R

R

E

A

H

A

L

L

M

E

E

K

A

A

V

V

Q

A

L

Q

R

R

L

V

E

T

-

P

A

G

D

D

V

T

P

P

V

L

A

-

C

V

Y
x
V

L
|
L

S
|
S

G

G

G
|
G

I
|
I

D
|
D

S
|
S

C

S

I

-

T

-

L

-

G

G

L

V

A

A

A

C

S

A

Q

H

Q

R

S

A

P

A

V

G

K

E

A

L

F

D

T

T

I

V

G
x
S

F
x
M

D

G

D

T

A

D

A

T

Y

S

D

N

E

E

T

F

A

R

I

E

A

A

R

R

E

A

M

V

A

V

E

D

S

H

V

L

G

R

A

T

D

R

Q

H

D

R

I

E

M

I

T

T

L

I

Q

P

A

T

D

T

H

E

L

L

Y

L

D

A

N

Q

L

L

V

P

E

Y

T

A

L

V

W

W

H

A

T

S

E

E

R

S

S

V

I

D

Y

P

N
x
D

T

I

L

I

G

E

V
x
Y

A

L

K

L

L

P

L
|
L

M

T

S

A

R

L

H

Y

V

R

N

A

E

L

A

D

G

G

-

P

Y

E

K

R

V

R

V

I

V

L

T

T

G
|
G

E
x
Y

G
|
G

S

A

D
|
D

E
x
I

L

P

F

L

A

G

G

G

Y

-

P

-

A

-

F
x
M

R

H

R

R

D

E

M

D

F

R

L

L

H

P

G

A

L

L

D

D

T

T

L

V

P

L

P

A

A

H

E
x
D

R

M

A

A

S

T

W

F

E

D

Q

G

L

L

active site: A73 (≠ N79), G74 (= G80), D319 (≠ N353), Y345 (≠ E378)
binding diphosphomethylphosphonic acid adenosyl ester: V244 (≠ Y275), L245 (= L276), S246 (= S277), G248 (= G279), I249 (= I280), D250 (= D281), S251 (= S282), S269 (≠ G303), M270 (≠ F304), L327 (= L361), G344 (= G377), Y345 (≠ E378), D348 (= D381)
binding n2-(carboxyethyl)-l-arginine: Y323 (≠ V357), Y345 (≠ E378), G346 (= G379), D348 (= D381), I349 (≠ E382), M354 (≠ F390), D370 (≠ E406)
binding magnesium ion: D250 (= D281), D348 (= D381)

Sites not aligning to the query:

active site: 379, 440
binding diphosphomethylphosphonic acid adenosyl ester: 420, 440
binding n2-(carboxyethyl)-l-arginine: 379

1mbzA Beta-lactam synthetase with trapped intermediate (see paper)
25% identity, 57% coverage: 33:413/672 of query aligns to 5:369/496 of 1mbzA

query
sites
1mbzA

P

P

D

A

G

A

F

F

G

G

Y

F

R

L

V

A

Q

S

Q

A

D

R

R

T

G

G

V

G

G

P

F

V

S

F

H

A

A

T

R

R

L

G

S

S

I

H

I

T

D

D

L

I

D

D

E

T

D

P

R

Q

G

G

R

E

Q

R

-

S

-

L

-

A

-

T

-

L

-

V

-

H

-

A

P

P

F

S

V

V

M

A

P

P

D

D

N

R

S

A

I

V

-

A

-

R

-

S

-

L

-

T

-

G

-

A

-

P

M

T

T

T

A

A

V

V

-

L

N
x
A

G
|
G

E

E

L

I

Y

Y

D

N

Y

R

K

D

R

E

I

L

R

-

A

-

D

-

L

L

T

S

L

V

R

L

G

P

V

A

K

G

F

P

R

A

T

P

K

E

S

G

D

D

S

A

E

E

M

L

V

V

M

L

H

R

L

L

Y

L

R

E

R

R

V

Y

G

D

L

L

E

H

D

-

A

A

I

F

P

R

H

L

F

V

R

N

G

G

E

R

F

F

A

A

I

T

S

V

L

V

Y

-

D

-

R

R

E

T

H

G

D

D

R

R

L

V

V

L

L

L

I

A

R

T

D

D

R

H

F

A

G

G

V

S

K

V

P

P

L

L

Y

Y

F

T

T

C

E

V

A

A

N

P

G

G

A

E

F

V

V

R

F

A

G

S

S

T

E

E

L

A

K

K

V

A

L

L

F

A

A

A

H

H

P

G

D

-

V

-

P

-

R

-

F

-

S

-

D

-

T

-

G

-

L

-

F

F

H

P

Q

L

L

A

M

D

Q

A

T

R

I

R

V

V

P

A

G

G

T

L

T

T

A

-

F

-

E

-

G

G

V

V

H

Y

Q

Q

V

V

K

P

P

A

G

G

H

A

M

V

V

M

I

D

V

I

E

D

R

L

A

G

H

S

G

G

K

-

L

-

K

T

V

A

R

V

Q

T

E

H

K

R

Y

T

W

W

D

T

M

-

D

-

F

-

P

P

L

G

A

L

A

S

E

R

R

R

-

I

-

L

P

P

E

E

H

G

D

E

D

-

T

-

D

-

E

-

Y

-

W

A

I

V

E

A

G

A

V

V

R

R

E

A

H

A

L

L

M

E

E

K

A

A

V

V

Q

A

L

Q

R

R

L

V

E

T

-

P

A

G

D

D

V

T

P

P

V

L

A

-

C

V

Y
x
V

L
|
L

S
|
S

G

G

G
|
G

I

I

D
|
D

S
|
S

C

S

I

-

T

-

L

-

G

G

L

V

A

A

A

C

S

A

Q

H

Q

R

S

A

P

A

V

G

K

E

A

L

F

D

T

T

I

V

G
x
S

F
x
M

D

G

D

T

A

D

A

T

Y

S

D

N

E

E

T

F

A

R

I

E

A

A

R

R

E

A

M

V

A

V

E

D

S

H

V

L

G

R

A

T

D

R

Q

H

D

R

I

E

M

I

T

T

L

I

Q

P

A

T

D

T

H

E

L

L

Y

L

D

A

N

Q

L

L

V

P

E

Y

T

A

L

V

W

W

H

A

T

S

E

E

R

S

S

V

I

D

Y

P

N
x
D

T

I

L

I

G

E

V
x
Y

A

L

K

L

L

P

L
|
L

M

T

S

A

R

L

H

Y

V

R

N

A

E

L

A

D

G

G

-

P

Y

E

K

R

V

R

V

I

V

L

T

T

G
|
G

E
x
Y

G
|
G

S

A

D
|
D

E
x
I

L

P

F

L

A

G

G

G

Y

-

P

-

A

-

F

M

R

H

R

R

D

E

M

D

F

R

L

L

H

P

G

A

L

L

D

D

T

T

L

V

P

L

P

A

A

H

E
x
D

R

M

A

A

S

T

W

F

E

D

Q

G

L

L

active site: A69 (≠ N79), G70 (= G80), D311 (≠ N353), Y337 (≠ E378)
binding arginine-n-methylcarbonyl phosphoric acid 5'-adenosine ester: V236 (≠ Y275), L237 (= L276), S238 (= S277), S243 (= S282), S261 (≠ G303), M262 (≠ F304), Y315 (≠ V357), L319 (= L361), G336 (= G377), Y337 (≠ E378), G338 (= G379), D340 (= D381), I341 (≠ E382), D362 (≠ E406)
binding magnesium ion: D242 (= D281), D340 (= D381)
binding pyrophosphate 2-: S238 (= S277), G240 (= G279), D242 (= D281), S243 (= S282), D340 (= D381)

Sites not aligning to the query:

active site: 371, 432
binding arginine-n-methylcarbonyl phosphoric acid 5'-adenosine ester: 371, 432, 434, 435
binding pyrophosphate 2-: 412, 432, 433

Query Sequence

>WP_006746648.1 NCBI__GCF_000227685.2:WP_006746648.1
MCGIAGIFHADPERRVDPETLVAMAAIQYHRGPDGFGYRVQQDRGVGFSHARLSIIDLDE
DRGRQPFVMPDNSIMTAVNGELYDYKRIRADLTLRGVKFRTKSDSEMVMHLYRRVGLEDA
IPHFRGEFAISLYDREHDRLVLIRDRFGVKPLYFTEANGAFVFGSELKVLFAHPDVPRRF
SDTGLFHQLMQTIVPGTTAFEGVHQVKPGHMVIVERAHGKLKVRQEKYWDMDFPLAAERP
EHDDTDEEYWIEGVREHLMEAVQLRLEADVPVACYLSGGIDSCITLGLAAASQQSPVKAF
TIGFDDAAYDETAIAREMAESVGADQDIMTLQADHLYDNLVETLWHTERSIYNTLGVAKL
LMSRHVNEAGYKVVVTGEGSDELFAGYPAFRRDMFLHGLDTLPPAERASWEQLLAESNKL
FSGAMLAENELDDPALTRLVGFTPSCLQPWLAAADHVPGLLAPGRRAALAGYAPGAAIAS
ALDADMLEGRHPLDKAQYVWIKTMLEGQILTWGGDRVDMANSMEARPPFLDHHLAEFAAQ
LPPSMRIKGRTEKYVLREAMKGLLPRVLYEREKFAFMAPPAHTDPKKWAAMKALADQYLS
TEAIEAAGLLDPEGVARLFALHEAEDTPVATQVQLDAVINHMISVQVLHEHFVAADIPRV
ARRKAEALGWRA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory