SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_006747898.1 NCBI__GCF_000227685.2:WP_006747898.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 13 hits to proteins with known functional sites (download)

3vayA Crystal structure of 2-haloacid dehalogenase from pseudomonas syringae pv. Tomato dc3000 (see paper)
39% identity, 89% coverage: 15:236/249 of query aligns to 2:223/230 of 3vayA

query
sites
3vayA

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binding magnesium ion: D8 (= D21), D10 (= D23), D180 (= D192)

6q7oA Crystal structure of oe1 (see paper)
34% identity, 36% coverage: 120:208/249 of query aligns to 103:196/230 of 6q7oA

query
sites
6q7oA

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binding calcium ion: D180 (= D192)

Sites not aligning to the query:

binding calcium ion: 8, 10

6q7nA Crystal structure of bh32 alkylated with the mechanistic inhibitor 2- bromoacetophenone (see paper)
34% identity, 36% coverage: 120:208/249 of query aligns to 103:196/230 of 6q7nA

query
sites
6q7nA

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Sites not aligning to the query:

binding 1-phenylethanone: 23, 68, 88, 91, 92, 95

8bp1A Crystal structure of bhmehis1.0, an engineered enzyme for the morita- baylis-hillman reaction (see paper)
36% identity, 36% coverage: 120:208/249 of query aligns to 103:196/231 of 8bp1A

query
sites
8bp1A

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binding magnesium ion: L122 (≠ S139), N123 (= N140), R124 (≠ G141), D180 (= D192)

Sites not aligning to the query:

binding magnesium ion: 8, 10

4knvA The crystal structure of apo human hdhd4 from se-mad (see paper)
23% identity, 92% coverage: 15:242/249 of query aligns to 1:235/241 of 4knvA

query
sites
4knvA

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binding magnesium ion: D7 (= D21), D9 (= D23), D184 (= D192)
binding phosphate ion: D7 (= D21), L8 (= L22), D9 (= D23), T126 (≠ S139), N127 (= N140), K159 (= K167)

4ygrA Crystal structure of had phosphatase from thermococcus onnurineus (see paper)
32% identity, 32% coverage: 133:212/249 of query aligns to 103:186/215 of 4ygrA

query
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4ygrA

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active site: T109 (≠ S139), S110 (≠ N140), G111 (= G141), K141 (= K167), G165 (= G191), D166 (= D192), L168 (≠ A194), Q170 (≠ C196), D171 (= D197)
binding magnesium ion: D166 (= D192)

Sites not aligning to the query:

active site: 7, 9, 15, 38
binding magnesium ion: 7, 9
binding 2-[n-cyclohexylamino]ethane sulfonic acid: 9, 50, 53, 58, 58, 61

3qnmA Haloalkane dehalogenase family member from bacteroides thetaiotaomicron of unknown function
23% identity, 80% coverage: 13:212/249 of query aligns to 1:205/231 of 3qnmA

query
sites
3qnmA

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binding magnesium ion: D9 (= D21), D11 (= D23), D185 (= D192)

8ywoA Crystal structure of l-azetidine-2-carboxylate hydrolase soaked in (s)-azetidine-2-carboxylic acid
26% identity, 47% coverage: 120:235/249 of query aligns to 102:230/240 of 8ywoA

query
sites
8ywoA

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I

M

binding (2S)-azetidine-2-carboxylic acid: N122 (= N140), H184 (≠ C196)

Sites not aligning to the query:

binding (2S)-azetidine-2-carboxylic acid: 14, 20

B2Z3V8 L-azetidine-2-carboxylate hydrolase; A2CH; AC hydrolase; EC 3.5.-.- from Pseudomonas sp. (strain A2C) (see paper)
26% identity, 47% coverage: 120:235/249 of query aligns to 102:230/240 of B2Z3V8

query
sites
B2Z3V8

P

P

E

D

A

T

A

V

S

E

V

A

L

L

D

Q

T

Y

L

L

S

K

R

K

H

H

F

Y

Q

K

I

L

G

V

A

I

I

L

S

S

N

N

G

I

N

D

A

R

D

N

V

E

F

F

R

K

-

L

-

S

-

N

T

A

P

K

L

L

G

G

P

V

Y

E

F

F

D

D

F

H

A

I

L

I

P

T

A

A

A

Q

E

D

A

V

G

G

A

S

A

Y

K

K

P

P

D

N

P

P

E

N

I

N

F

F

R

T

A

Y

-

M

-

I

-

D

A

A

A

L

R

A

R

K

A

A

G

G

V

I

R

E

P

K

E

K

S

D

M

I

L

L

H

H

I

T

G

A

D

E

D

S

A

L

V

Y

C

H

D

D

V

H

L

I

G

P

A

A

R

N

N

D

A

A

G

G

L

L

H

V

A

S

G

A

W

W

F

I

-

Y

-

R

-

R

-

H

-

G

-

K

-

E

N

G

P

Y

G

G

T

A

M

T

P

H

W

V

A

P

Q

S

R

R

E

M

P

P

T

N

P

V

D

D

L

F

T

R

F

F

G

N

R

S

L

M

G

G

Q

E

I

M

Sites not aligning to the query:

12 D→A: Loss of catalytic activity.

2no5B Crystal structure analysis of a dehalogenase with intermediate complex (see paper)
30% identity, 41% coverage: 107:207/249 of query aligns to 86:191/226 of 2no5B

query
sites
2no5B

D

D

V

R

F

L

W

M

E

S

A

A

R

Y

N

K

E

E

V

L

D

S

P

A

D

Y

P

P

E

D

A

A

A

A

S

E

V

T

L

L

D

E

T

K

L

L

-

K

S

S

R

A

H

G

F

Y

Q

I

I

V

G

A

A

I

I

L

S
|
S

N
|
N

G

G

N

N

A

D

D

E

V

M

F

L

R

Q

-

A

-

A

-

L

-

K

-

A

T

S

P

K

L

L

G

D

P

R

Y

V

F

L

D

D

F

S

A

C

L

L

P

S

A

A

A

D

E

D

A

L

G

K

A

I

A

Y

K
|
K

P

P

D

D

P

P

E

R

I

I

F

Y

R

Q

A

F

A

A

A

C

R

D

R

R

A

L

G

G

V

V

R

N

P

P

E

N

S

E

M

V

L

C

H

F

I

V

G
x
S

D

S

D
x
N

A
|
A

V

-

C
x
W

D
|
D

V

L

L

G

G

G

A

A

R

G

N

K

A

F

G

G

L

F

H

N

active site: S119 (= S139), N120 (= N140), K152 (= K167), S176 (≠ G191), N178 (≠ D193), W180 (≠ C196), D181 (= D197)
binding (2s)-2-chloropropanoic acid: N178 (≠ D193), A179 (= A194), W180 (≠ C196)

Sites not aligning to the query:

active site: 11, 15, 42
binding (2s)-2-chloropropanoic acid: 20, 35, 38, 39

Q51645 (S)-2-haloacid dehalogenase 4A; 2-haloalkanoic acid dehalogenase IVA; Halocarboxylic acid halidohydrolase IVA; L-2-haloacid dehalogenase IVA; EC 3.8.1.2 from Burkholderia cepacia (Pseudomonas cepacia) (see 2 papers)
30% identity, 41% coverage: 107:207/249 of query aligns to 86:191/231 of Q51645

query
sites
Q51645

D

D

V

R

F

L

W

M

E

S

A

A

R

Y

N

K

E

E

V

L

D

S

P

A

D

Y

P

P

E

D

A

A

A

A

S

E

V

T

L

L

D

E

T

K

L

L

-

K

S

S

R

A

H

G

F

Y

Q

I

I

V

G

A

A

I

I

L

S
|
S

N
|
N

G

G

N

N

A

D

D

E

V

M

F

L

R

Q

-

A

-

A

-

L

-

K

-

A

T

S

P

K

L

L

G

D

P

R

Y

V

F

L

D

D

F

S

A

C

L

L

P

S

A

A

A

D

E

D

A

L

G

K

A

I

A

Y

K

K

P

P

D

D

P

P

E

R

I

I

F

Y

R

Q

A

F

A

A

A

C

R

D

R

R

A

L

G

G

V

V

R

N

P

P

E

N

S

E

M

V

L

C

H

F

I

V

G

S

D

S

D

N

A

A

V

-

C

W

D

D

V

L

L

G

G

G

A

A

R

G

N

K

A

F

G

G

L

F

H

N

SN 119:120 (= SN 139:140) binding an (S)-2-haloacid

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
11 active site, Nucleophile
12:13 binding an (S)-2-haloacid
42 binding an (S)-2-haloacid

2x4dA Crystal structure of human phospholysine phosphohistidine inorganic pyrophosphate phosphatase lhpp
36% identity, 26% coverage: 150:213/249 of query aligns to 170:241/270 of 2x4dA

query
sites
2x4dA

L

V

G

G

P

P

Y

Y

-

M

-

K

-

A

F

L

D

E

F

Y

A

A

L

C

P

G

A

I

A

K

E

A

A

E

G

V

A

V

A

G

K
|
K

P

P

D

S

P

P

E

E

I

F

F

F

R

K

A

S

A

A

A

L

R

Q

R

A

A

I

G

G

V

V

R

E

P

A

E

H

S

Q

M

A

L

V

H

M

I

I

G

G

D
|
D

D

D

A

I

V

V

C

G

D

D

V

V

L

G

G

G

A

A

R

Q

N

R

A

C

G

G

L

M

H

R

A

A

-

L

-

Q

-

V

-

R

-

T

G

G

W

K

F

F

N

R

P

P

binding magnesium ion: D215 (= D192)
binding pyrophosphate 2-: K190 (= K167)

Sites not aligning to the query:

binding magnesium ion: 18, 20
binding pyrophosphate 2-: 18, 19, 20, 55, 56, 57

2x4dB Crystal structure of human phospholysine phosphohistidine inorganic pyrophosphate phosphatase lhpp
36% identity, 26% coverage: 150:213/249 of query aligns to 122:193/213 of 2x4dB

query
sites
2x4dB

L

V

G

G

P

P

Y

Y

-

M

-

K

-

A

F

L

D

E

F

Y

A

A

L

C

P

G

A

I

A

K

E

A

A

E

G

V

A

V

A

G

K
|
K

P

P

D

S

P

P

E

E

I

F

F

F

R

K

A

S

A

A

A

L

R

Q

R

A

A

I

G

G

V

V

R

E

P

A

E

H

S

Q

M

A

L

V

H

M

I

I

G

G

D
|
D

D

D

A

I

V

V

C

G

D

D

V

V

L

G

G

G

A

A

R

Q

N

R

A

C

G

G

L

M

H

R

A

A

-

L

-

Q

-

V

-

R

-

T

G

G

W

K

F

F

N

R

P

P

binding magnesium ion: D167 (= D192)
binding pyrophosphate 2-: K142 (= K167)

Sites not aligning to the query:

binding magnesium ion: 3, 5
binding pyrophosphate 2-: 3, 4, 5, 26, 27, 28

Query Sequence

>WP_006747898.1 NCBI__GCF_000227685.2:WP_006747898.1
MNAHPAAVAAIQVRVRCITFDLDDTLWPVMPAIHRAEARFYDWLRIHAPQVCERYTPQAL
IDARSGFMRAAPASERHDLTRLRKRWLRALALETGANPARLESEGFDVFWEARNEVDPDP
EAASVLDTLSRHFQIGAISNGNADVFRTPLGPYFDFALPAAEAGAAKPDPEIFRAAARRA
GVRPESMLHIGDDAVCDVLGARNAGLHAGWFNPGTMPWAQREPTPDLTFGRLGQIPVRLG
LIRPAPLRP

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory