SitesBLAST

M1 (= M1) modified: Initiator methionine, Removed
C2 (= C2) mutation C->A,S: Loss of glutamine-dependent activity but no effect on ammonia-dependent asparagine synthetase activity.
H30 (vs. gap) mutation to A: 4,5-fold decrease in glutamine affinity.
D34 (= D33) mutation D->N,E: Little effect on the kinetic properties.
H81 (≠ F81) mutation to A: 5-fold decrease in glutamine affinity.
A105 (≠ K106) mutation to H: Little effect on the kinetic properties.
E349 (≠ Q370) mutation E->A,Q: Loss of glutamine- and ammonia-dependent synthetase activity, but still exhibits glutaminase activity.; mutation to D: 5-fold increase in affinity for aspartate when assaying both the glutamine- and ammonia-dependent synthetase reactions, and 2-fold decrease in kcat for these reactions. Modifies the product glutamate/asparagine stoichiometry.

1ct9A Crystal structure of asparagine synthetase b from escherichia coli (see paper)
30% identity, 84% coverage: 27:523/595 of query aligns to 27:433/497 of 1ct9A

query
sites
1ct9A

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F

active site: L50 (= L50), N74 (= N75), G75 (= G76), T305 (≠ M344), R308 (≠ Q347), E332 (≠ Q370), M366 (≠ E434)
binding adenosine monophosphate: L232 (= L265), L233 (= L266), S234 (= S267), S239 (= S272), A255 (≠ T291), V256 (= V292), D263 (≠ E299), M316 (≠ L355), S330 (= S368), G331 (= G369), E332 (≠ Q370)
binding glutamine: R49 (= R49), L50 (= L50), I52 (= I52), V53 (≠ L53), N74 (= N75), G75 (= G76), E76 (≠ A77), D98 (= D100)

Sites not aligning to the query:

active site: 1
binding glutamine: 1

P78753 Probable asparagine synthetase [glutamine-hydrolyzing]; Glutamine-dependent asparagine synthetase; EC 6.3.5.4 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
29% identity, 84% coverage: 1:501/595 of query aligns to 1:435/557 of P78753

query
sites
P78753

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S391 (≠ D456) modified: Phosphoserine

Sites not aligning to the query:

489 modified: Phosphoserine

P08243 Asparagine synthetase [glutamine-hydrolyzing]; Cell cycle control protein TS11; Glutamine-dependent asparagine synthetase; EC 6.3.5.4 from Homo sapiens (Human) (see 7 papers)
25% identity, 92% coverage: 1:547/595 of query aligns to 1:493/561 of P08243

query
sites
P08243

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V

-

E

-

M

-

V

-

K

W

Y

Q

H

L

H

C

C

A

R

R

D

R
x
V

P

P

E

L

Q

H

A

A

I

L

D

Y

D

D

D

N

E

V

W

E

R

K

-

L

-

F

-

P

-

G

-

F

-

E

-

I

E

E

R

T

I

V

E

K

A

N

G

N

L

L

R

R

-

I

-

L

-

F

-

N

R

N

A

A

V

V

R

K

R

R

L

L

E

-

V

M

A

T

D

D

V

R

P

R

V

I

G

G

V

C

L

L

S

S

G

G

L

L

D

D

S

S

L

L

L

V

V

A

A

A

L

T

A

L

V

L

E

K

E

Q

G

L

A

K

Q

E

-

A

-

Q

-

V

-

Q

-

Y

D

P

L

L

R

Q

T

T

F

F

T

A

V

I

G

G

F

M

E

E

D

D

Q

S

P

P

E

D

E

L

K

L

G

A

S

A

E

R

F

-

E

-

F

-

S

-

D

-

P

K

V

V

A

A

E

D

R

H

Y

I

A

G

T

S

D

E

H

H

Q

Y

R

E

T

V

L

L

I

F

P

N

N

S

S

E

E

E

V

G

L

I

R

Q

R

A

L

L

P

D

E

E

A

V

V

I

A

F

A

S

M

L

A

E

-

T

-

Y

E

D

P

I

M

T

F

T

G

V

Q

R

D

A

A

S

V

V

A

G

F

M

Y

Y

L

L

L

I

A

S

E

K

Q

Y

V

I

S

R

Q

K

H

N

V

T

K

D

-

S

-

V

V

V

V

I

Q
x
F

S

S

G

G

Q

E

G

G

A

S

D

D

E

E

V

L

F

T

G

Q

G

G

Y

Y

F

I

W

Y

Y

F

P

H

K

K

M

-

A

-

E

-

R

-

S

-

G

-

D

-

W

-

A

-

E

-

R

-

F

-

R

-

K

-

H

-

Y

-

F

-

D

-

R

-

D

-

P

-

E

-

M

-

R

-

E

-

L

-

A

-

A

A

P

P

P

S

P

P

N

E

D

-

L

-

A

-

G

-

E

-

W

-

L

-

R

K

A

A

E

E

L

E

A

E

M

S

L

E

E

R

T

L

D

L

R

R

A

E

D

L

S

Y

F

L

I

F

D

D

A

V

V

L

L

R

A

A

L

-

D

-

V

-

T

-

L

-

I

-

V

-

D

-

P

-

V

-

K

-

R

-

V

-

D

D

N

R

M

T

T

T

M

A

A

A

F

H

G

G

L

L

E

E

A

L

R

R

V

V

P

P

F

F

L

L

D

D

H

H

E

R

L

F

V

S

E

S

T

Y

A

Y

A

L

A

S

M

L

P

P

H

E

L

M

K

R

L

I

G

P

D

K

G

N

G

G

-

I

-

E

K

K

K

H

V

L

L

L

K

R

Q

E

I

T

A

F

R

E

-

D

-

S

G

N

R

L

L

I

P

P

D

K

A

E

V

I

L

L

D

W

R

R

P

P

K

K

G

E

Y

A

F

F

P

S

V

-

P

D

A

G

L

I

K

T

Y

S

V

V

R

K

G

N

D

S

F

W

L

F

E

K

F

I

M

L

R

Q

D

E

I

Y

L

V

G

E

S

H

Q

Q

A

V

C2 (= C2) active site, For GATase activity; mutation to A: Loss of the glutamine-dependent asparagine synthetase activity, while the ammonia-dependent activity remained unaffected.
A6 (≠ G6) to E: in ASNSD; dramatic reduction in protein abundance; dbSNP:rs398122975
V210 (≠ R229) to E: in dbSNP:rs1049674
F362 (≠ Q367) to V: in ASNSD; dramatic reduction in protein abundance; dbSNP:rs398122973

Sites not aligning to the query:

550 R → C: in ASNSD; increases level of protein abundance; dbSNP:rs398122974

6gq3A Human asparagine synthetase (asns) in complex with 6-diazo-5-oxo-l- norleucine (don) at 1.85 a resolution (see paper)
25% identity, 87% coverage: 2:519/595 of query aligns to 1:453/509 of 6gq3A

query
sites
6gq3A

C
|
C

G

G

I

I

A
x
W

G

A

E

L

F

F

V

-

W

G

G

S

D

D

R

D

R

C

A

L

D

S

V

V

D

Q

A

C

V

L

A

S

R

A

M

M

L

-

P

-

A

K

L

I

A

A

R

H

R

R

G

G

P

P

D

D

A

A

-

F

-

R

-

F

D

E

G

N

V

V

W

N

A

G

S

Y

G

T

R

N

A

C

L

F

G

G

H

F

R

H

R
|
R

L
|
L

S

A

I
x
V

L
x
V

D

D

L

-

S

P

Q

L

R

F

A

G

H

M

Q

Q

P

P

M

I

-

R

F

V

D

K

P

K

E

Y

T

P

G

Y

L

L

A

W

L

L

V

C

F
x
Y

N
|
N

G
|
G

A
x
E

I

I

Y

Y

N

N

F

H

R

K

T

K

L

M

R

Q

A

Q

E

H

L

F

E

E

R

-

G

-

H

F

R

E

F

Y

F

Q

S

T

S

K

G
x
V

D
|
D

T

G

E

E

V

I

I

I

L

L

K

H

G

L

Y

Y

A

D

E

K

W

G

G

G

-

I

E

E

G

Q

V

T

V

I

A

C

R

M

L

L

H

D

G

G

M

V

F

F

A

A

F

F

A

V

L

L

F

L

D

D

S

T

R

A

T

N

Q

K

K

K

I

V

L

F

L

L

A

G

R

R

D

D

R

T

M

Y

G

G

I

V

K

R

P

P

L

L

Y

F

L

K

A

A

R

M

S

T

G

E

H

D

G

G

I

F

R

L

F

-

A

-

S

-

N

-

T

-

Q

-

A

-

L

-

D

-

S

-

G

-

V

-

D

-

P

-

A

A

L

V

D

C

P

S

E

E

A

A

L

K

H

G

L

L

L

V

F

T

S

L

L

K

H

H

A

S

V

A

V

T

P

P

A

F

P

L

R

K

T

-

V

-

L

-

R

-

G

-

I

V

R

E

K

P

L

F

Q

L

P

P

G

G

H

H

Y

Y

L

E

L

V

I

L

E

D

A

L

D

K

G

P

H

N

E

G

H

K

L

V

E

A

R

S

Y

V

-

E

-

M

-

V

-

K

W

Y

Q

H

L

H

C

C

A

-

R

R

R

D

P

V

E

F

Q

P

A

G

I

F

D

E

D

I

D

E

E

T

W

V

R

K

E

N

R

N

I

L

E

R

A

I

G

L

L

F

R

N

A

A

V

V

R

K

R

R

L

L

E

-

V

M

A

T

D

D

V

R

P

R

V

I

G

G

V

C

L

L

S

S

G

G

L

L

D

D

S

S

L

L

L

V

V

A

A

A

L

T

A

L

V

L

E

K

E

Q

G

L

A

K

Q

E

-

A

-

Q

-

V

-

Q

-

Y

D

P

L

L

R

Q

T

T

F

F

T

A

V

I

G

G

F

M

E

E

D

D

Q

S

P

P

E

D

E

L

K

L

G

A

S

A

E

R

F

-

E

-

F

-

S

-

D

-

P

K

V

V

A

A

E

D

R

H

Y

I

A

G

T

S

D

E

H

H

Q

Y

R

E

T

V

L

L

I

F

P

N

N

S

S

E

E

E

V

G

L

I

R

Q

R

A

L

L

P

D

E

E

A

V

V

I

A

F

A

S

M

L

A

E

-

T

-

Y

E

D

P

I

M
x
T

F

T

G

V

Q
x
R

D

A

A

S

V

V

A

G

F

M

Y

Y

L

L

L

I

A

S

E

K

Q

Y

V

I

S

R

Q

K

H

N

V

T

K

D

-

S

-

V

V

V

V

I

Q

F

S

S

G

G

Q

E

G

G

A

S

D

D

E

E

V

L

F

T

G

Q

G

G

Y

Y

F

I

W

Y

Y

F

P

H

K

K

M

-

A

-

E

-

R

-

S

-

G

-

D

-

W

-

A

-

E

-

R

-

F

-

R

-

K

-

H

-

Y

-

F

-

D

-

R

-

D

-

P

-

E

-

M

-

R

-

E

-

L

-

A

-

A

A

P

P

P

S

P

P

N

E

D

-

L

-

A

-

G

-

E

-

W

-

L

-

R

K

A

A

E

E

L

E

A

E

M

S

L

E

E

R

T

L

D

L

R

R

A

E

D

L

S

Y

F

L

I

F

D

D

A

V

V

L

L

R

A

A

L

-

D

-

V

-

T

-

L

-

I

-

V

-

D

-

P

-

V

-

K

-

R

-

V

-

D

D

N

R

M

T

T

T

M

A

A

A

F

H

G

G

L

L

E

E

A

L

R

R

V

V

P

P

F

F

L

L

D

D

H

H

E

R

L

F

V

S

E

S

T

Y

A

Y

A

L

A

S

M

L

P

P

H

E

L

M

K

R

L

I

G

P

D

K

G

N

G

G

-

I

-

E

K

K

K

H

V

L

L

L

K

R

Q

E

I

T

A

F

R

E

-

D

-

S

G

N

R

L

L

I

P

P

D

K

A

E

V

I

L

L

D

W

R

R

P

P

active site: W4 (≠ A5), L49 (= L50), N74 (= N75), G75 (= G76), T324 (≠ M344), R327 (≠ Q347)
binding 5-oxo-l-norleucine: C1 (= C2), R48 (= R49), V51 (≠ I52), V52 (≠ L53), Y73 (≠ F74), N74 (= N75), G75 (= G76), E76 (≠ A77), V95 (≠ G99), D96 (= D100)

Q9XB61 Carbapenam-3-carboxylate synthase; Carbapenam-3-carboxylate ligase; EC 6.3.3.6 from Pectobacterium carotovorum subsp. carotovorum (Erwinia carotovora subsp. carotovora) (see 3 papers)
26% identity, 55% coverage: 53:378/595 of query aligns to 35:353/503 of Q9XB61

query
sites
Q9XB61

L

L

D

F

L

I

S

E

Q

Q

R

N

A

G

H

H

Q

Y

P

Q

M

K

F

C

D

E

P

M

E

E

T

R

G

G

L

T

A

A

L

Y

V

L

F

I

N

-

G

G

A

S

I

L

Y

Y

N

N

F

-

R

R

T

T

L

F

R

L

A

I

E

G

L

L

E

A

R

G

-

V

-

W

R

E

G

G

H

E

R

A

F

Y

F

L

S

A

S

N

G

-

D

D

T

A

E

E

V

L

I

L

L

A

K

L

G

L

Y

F

A

T

E

R

W

L

G

G

E

A

G

N

V

A

V

L

A

A

R

L

L

A

H

E

G

G

M

D

F

F

A

C

F

F

A

-

L

F

F

I

D

D

S

E

R

P

T

N

Q

G

K

E

I

L

L

T

L

V

A

I

R

T

D

E

R

S

M

R

G

G

I

F

K

S

P

P

L

V

Y

H

L

V

A

V

R

Q

S

G

G

K

H

K

G

A

I

-

R

-

F

W

A

M

S

T

N

N

T

S

Q

L

A

K

L

L

L

V

D

T

S

A

G

A

G

E

V

G

D

E

P

G

A

A

L

L

-

W

-

F

D

E

P

E

E

E

A

A

L

L

H

V

L

C

L

Q

F

S

S

L

L

M

H

R

A

A

V

D

V

T

P

Y

A

T

P

P

R

-

T

-

V

-

L

V

R

K

G

N

I

A

R

Q

K

R

L

L

Q

K

P

P

G

G

-

A

-

V

H

H

Y

V

L

L

L

T

I

H

E

D

A

S

D

E

G

G

H

Y

E

S

H

F

L

V

E

E

-

S

R

R

Y

T

W

L

Q

T

L

T

C

P

A

A

R

S

R

N

P

Q

E

L

Q

L

A

A

I

L

D

P

D

-

E

-

W

-

R

R

E

E

R

P

I

L

E

L

A

A

G

L

L

I

R

D

R

R

A

Y

V

L

R

N

R

A

R

P

L

L

E

E

V

-

A

-

D

D

V

L

-

A

-

P

-

R

-

F

-

D

P

T

V

V

G

G

V

I

L
x
P

L
|
L

S
|
S

G
|
G

L
|
L

D
|
D

S
|
S

S

S

L

L

L

V

V

T

A

A

L

L

A

A

V

S

E

R

E

H

G

-

A

F

Q

K

D

K

L

L

R

N

T

T

F

Y

T

S

V
x
I

G

G

F

-

E

-

D

-

Q

-

P

-

E

T

E

E

K

L

G

S

S

N

E

E

F

F

E

E

F

F

S

S

D

Q

P

Q

V

V

A

A

E

D

R

A

Y

L

A

G

T

T

D

H

H

H

Q

Q

R

M

T

K

L

I

I

L

P

S

N

E

S

T

E

E

V

V

L

I

R

N

R

G

L

I

P

I

E

E

A

S

V

I

-

Y

A

N

A

E

M

I

A

F

E

D

P

G

M

L

F

S

G

A

Q

E

D

I

A

Q

V

S

A

G

F

L

Y

F

L

N

L

V

A

Y

E

R

Q

Q

V

A

S

Q

Q

G

H

Q

V

V

K

S

V

C

V

M

Q

L

S

T

G
|
G

Q
x
Y

G
|
G

A
x
S

D
|
D

E

L

V

L

F

F

G

G

244:251 (vs. 265:272, 88% identical) binding ATP
I270 (≠ V292) binding ATP
GYGSD 344:348 (≠ GQGAD 369:373) binding ATP
Y345 (≠ Q370) mutation to A: Loss of activity.; mutation to F: Reduces catalytic efficiency.
G346 (= G371) binding substrate

Sites not aligning to the query:

371 binding substrate
374 binding substrate
380 E→A: Loss of activity.; E→D: Reduces catalytic efficiency.; E→Q: Reduces catalytic efficiency.
421 binding ATP
443 mutation K->A,M: Loss of activity.
444:446 binding ATP

1q19A Carbapenam synthetase (see paper)
26% identity, 55% coverage: 53:378/595 of query aligns to 34:352/500 of 1q19A

query
sites
1q19A

L

L

D

F

L

I

S

E

Q

Q

R

N

A

G

H

H

Q

Y

P

Q

M

K

F

C

D

E

P

M

E

E

T

R

G

G

L

T

A

A

L

Y

V

L

F

I

N

-

G
|
G

A

S

I

L

Y

Y

N

N

F

-

R

R

T

T

L

F

R

L

A

I

E

G

L

L

E

A

R

G

-

V

-

W

R

E

G

G

H

E

R

A

F

Y

F

L

S

A

S

N

G

-

D

D

T

A

E

E

V

L

I

L

L

A

K

L

G

L

Y

F

A

T

E

R

W

L

G

G

E

A

G

N

V

A

V

L

A

A

R

L

L

A

H

E

G

G

M

D

F

F

A

C

F

F

A

-

L

F

F

I

D

D

S

E

R

P

T

N

Q

G

K

E

I

L

L

T

L

V

A

I

R

T

D

E

R

S

M

R

G

G

I

F

K

S

P

P

L

V

Y

H

L

V

A

V

R

Q

S

G

G

K

H

K

G

A

I

-

R

-

F

W

A

M

S

T

N

N

T

S

Q

L

A

K

L

L

L

V

D

T

S

A

G

A

G

E

V

G

D

E

P

G

A

A

L

L

-

W

-

F

D

E

P

E

E

E

A

A

L

L

H

V

L

C

L

Q

F

S

S

L

L

M

H

R

A

A

V

D

V

T

P

Y

A

T

P

P

R

-

T

-

V

-

L

V

R

K

G

N

I

A

R

Q

K

R

L

L

Q

K

P

P

G

G

-

A

-

V

H

H

Y

V

L

L

L

T

I

H

E

D

A

S

D

E

G

G

H

Y

E

S

H

F

L

V

E

E

-

S

R

R

Y

T

W

L

Q

T

L

T

C

P

A

A

R

S

R

N

P

Q

E

L

Q

L

A

A

I

L

D

P

D

-

E

-

W

-

R

R

E

E

R

P

I

L

E

L

A

A

G

L

L

I

R

D

R

R

A

Y

V

L

R

N

R

A

R

P

L

L

E

E

V

-

A

-

D

D

V

L

-

A

-

P

-

R

-

F

-

D

P

T

V

V

G

G

V

I

L
x
P

L
|
L

S
|
S

G

G

L

L

D
|
D

S
|
S

S

S

L

L

L

V

V

T

A

A

L

L

A

A

V

S

E

R

E

H

G

-

A

F

Q

K

D

K

L

L

R

N

T

T

F

Y

T
x
S

V
x
I

G

G

F

-

E

-

D

-

Q

-

P

-

E

T

E

E

K

L

G

S

S

N

E

E

F

F

E

E

F

F

S

S

D

Q

P

Q

V

V

A

A

E

D

R

A

Y

L

A

G

T

T

D

H

H

H

Q

Q

R

M

T

K

L

I

I

L

P

S

N

E

S

T

E

E

V

V

L

I

R

N

R

G

L

I

P

I

E

E

A

S

V

I

-

Y

A

N

A

E

M

I

A

F

E

D

P

G

M
x
L

F

S

G

A

Q
x
E

D

I

A

Q

V

S

A

G

F

L

Y

F

L

N

L

V

A

Y

E

R

Q

Q

V

A

S

Q

Q

G

H

Q

V

V

K

S

V

C

V

M

Q

L

S
x
T

G
|
G

Q
x
Y

G
|
G

A

S

D
|
D

E
x
L

V

L

F

F

G

G

active site: G56 (= G76), L318 (≠ M344), E321 (≠ Q347), Y344 (≠ Q370)
binding diphosphomethylphosphonic acid adenosyl ester: P243 (≠ L265), L244 (= L266), S245 (= S267), D249 (= D271), S250 (= S272), S268 (≠ T291), I269 (≠ V292), T342 (≠ S368), G343 (= G369), D347 (= D373)
binding (2s,5s)-5-carboxymethylproline: Y344 (≠ Q370), G345 (= G371), L348 (≠ E374)

Sites not aligning to the query:

active site: 379, 442
binding diphosphomethylphosphonic acid adenosyl ester: 442, 443, 444, 445
binding (2s,5s)-5-carboxymethylproline: 373, 379

1jgtB Crystal structure of beta-lactam synthetase (see paper)
26% identity, 54% coverage: 60:378/595 of query aligns to 49:353/500 of 1jgtB

query
sites
1jgtB

H

H

Q

A

P

P

M

S

F

V

D

A

P

P

E

D

T

R

G

A

L

V

A

A

L

R

V

S

F

L

N

T

G

G

A

A

-

P

-

T

-

A

-

V

-

L

-
x
A

-
x
G

-

E

I

I

Y

Y

N

N

F

R

R

D

T

E

L

L

R

L

A

S

E

V

L

L

E

P

R

A

R

G

G

P

H

-

R

-

F

-

F

A

S

P

S

E

G

G

D

D

T

A

E

E

V

L

I

V

L

L

K

R

G

L

Y

L

A

E

E

R

W

Y

G

D

E

L

G

H

V

A

V

F

A

R

R

L

L

V

H

N

G

G

M

R

F

F

A

A

F

T

A

V

L

V

F

-

D

-

S

-

R

R

T

T

-

G

Q

D

K

R

I

V

L

L

A

A

R

T

D

D

R

H

M

A

G

G

I

S

K

V

P

P

L

L

Y

Y

L

T

A

C

R

V

S

A

G

P

H

G

G

E

I

V

R

R

F

A

A

S

S

T

N

E

T

A

Q

K

A

A

L

L

L

A

D

A

S

H

G

-

V

-

D

-

P

-

A

-

L

R

D

D

P

P

E

K

A

G

L

F

H

P

L

L

L

A

F

D

S

A

L

R

H

R

A

V

V

A

V

G

P

-

A

-

P

-

R

-

T

-

V

-

L

L

R

T

G

G

I

V

R

Y

K

Q

L

V

Q

P

P

A

G

G

H

A

Y

V

L

M

L

D

I

I

E

D

-

L

A

G

D

S

G

G

H

T

E

A

H

V

L

T

E

H

R

R

Y

T

W

W

Q

T

L

P

C

G

A

L

R

S

R

R

P

-

E

-

Q

R

A

I

I

L

D

P

D

E

D

G

E

E

W

A

R

V

E

A

R

A

I

V

E

R

A

A

G

A

L

L

R

E

R

K

A

A

V

V

R

A

R

Q

R

R

L

V

E

T

V

P

A

G

D

D

V

T

P

P

V

L

G

-

V

V

L
x
V

L
|
L

S
|
S

G

G

G
|
G

L
x
I

D
|
D

S
|
S

S

S

L

G

L

V

V

A

A

A

L

C

A

A

V

-

E

H

E

R

G

A

A

A

Q

G

D

E

L

L

R

D

T

T

F

V

T
x
S

V
x
M

G

G

F

-

E

-

D

-

Q

-

P

-

E

T

E

D

K

T

G

S

S

N

E

E

F

F

E

R

F

E

S

A

D

R

P

A

V

V

A

V

E

D

R

H

Y

L

A

R

T

T

D

R

H

H

Q

R

R

E

T

I

L

T

I

I

P

P

N

T

S

T

E

E

V

L

L

L

R

A

R

Q

L

L

P

P

E

Y

A

A

V

V

A

W

A

A

-

S

-

E

M

S

A

V

E

D

P

P

M
x
D

F

I

G

I

Q

E

D
x
Y

A

L

V

L

A

P

F
x
L

Y

T

L

A

L

L

A

Y

E

R

Q

A

V

L

S

D

Q

G

H

P

V

E

K

R

V

R

V

I

Q

L

S

T

G
|
G

Q
x
Y

G
|
G

A

A

D
|
D

E
x
I

V

P

F

L

G

G

active site: A73 (vs. gap), G74 (vs. gap), D319 (≠ M344), Y345 (≠ Q370)
binding diphosphomethylphosphonic acid adenosyl ester: V244 (≠ L265), L245 (= L266), S246 (= S267), G248 (= G269), I249 (≠ L270), D250 (= D271), S251 (= S272), S269 (≠ T291), M270 (≠ V292), L327 (≠ F352), G344 (= G369), Y345 (≠ Q370), D348 (= D373)
binding n2-(carboxyethyl)-l-arginine: Y323 (≠ D348), Y345 (≠ Q370), G346 (= G371), D348 (= D373), I349 (≠ E374)
binding magnesium ion: D250 (= D271), D348 (= D373)

Sites not aligning to the query:

active site: 379, 440
binding diphosphomethylphosphonic acid adenosyl ester: 420, 440
binding n2-(carboxyethyl)-l-arginine: 354, 370, 379

1mbzA Beta-lactam synthetase with trapped intermediate (see paper)
26% identity, 54% coverage: 60:378/595 of query aligns to 45:345/496 of 1mbzA

query
sites
1mbzA

H

H

Q

A

P

P

M

S

F

V

D

A

P

P

E

D

T

R

G

A

L

V

A

A

L

R

V

S

F

L

N

T

G

G

A

A

-

P

-

T

-

A

-

V

-

L

-
x
A

-
x
G

-

E

I

I

Y

Y

N

N

F

R

R

D

T

E

L

L

R

L

A

S

E

V

L

L

E

P

R

A

R

G

G

P

H

-

R

-

F

-

F

A

S

P

S

E

G

G

D

D

T

A

E

E

V

L

I

V

L

L

K

R

G

L

Y

L

A

E

E

R

W

Y

G

D

E

L

G

H

V

A

V

F

A

R

R

L

L

V

H

N

G

G

M

R

F

F

A

A

F

T

A

V

L

V

F

-

D

-

S

-

R

R

T

T

-

G

Q

D

K

R

I

V

L

L

A

A

R

T

D

D

R

H

M

A

G

G

I

S

K

V

P

P

L

L

Y

Y

L

T

A

C

R

V

S

A

G

P

H

G

G

E

I

V

R

R

F

A

A

S

S

T

N

E

T

A

Q

K

A

A

L

L

L

A

D

A

S

H

G

G

G

-

V

-

D

-

P

-

A

-

L

-

D

-

P

-

E

-

A

-

L

-

H

-

L

-

F

F

S

P

L

L

H

-

A

-

V

-

V

A

P

D

A

A

P

R

R

R

T

V

V

A

-

G

L

L

R

T

G

G

I

V

R

Y

K

Q

L

V

Q

P

P

A

G

G

H

A

Y

V

L

M

L

D

I

I

E

D

-

L

A

G

D

S

G

G

H

T

E

A

H

V

L

T

E

H

R

R

Y

T

W

W

Q

T

L

P

C

G

A

L

R

S

R

R

P

-

E

-

Q

R

A

I

I

L

D

P

D

E

D

G

E

E

W

A

R

V

E

A

R

A

I

V

E

R

A

A

G

A

L

L

R

E

R

K

A

A

V

V

R

A

R

Q

R

R

L

V

E

T

V

P

A

G

D

D

V

T

P

P

V

L

G

-

V

V

L
x
V

L
|
L

S
|
S

G

G

G
|
G

L

I

D
|
D

S
|
S

S

S

L

G

L

V

V

A

A

A

L

C

A

A

V

-

E

H

E

R

G

A

A

A

Q

G

D

E

L

L

R

D

T

T

F

V

T
x
S

V
x
M

G

G

F

-

E

-

D

-

Q

-

P

-

E

T

E

D

K

T

G

S

S

N

E

E

F

F

E

R

F

E

S

A

D

R

P

A

V

V

A

V

E

D

R

H

Y

L

A

R

T

T

D

R

H

H

Q

R

R

E

T

I

L

T

I

I

P

P

N

T

S

T

E

E

V

L

L

L

R

A

R

Q

L

L

P

P

E

Y

A

A

V

V

A

W

A

A

-

S

-

E

M

S

A

V

E

D

P

P

M
x
D

F

I

G

I

Q

E

D
x
Y

A

L

V

L

A

P

F
x
L

Y

T

L

A

L

L

A

Y

E

R

Q

A

V

L

S

D

Q

G

H

P

V

E

K

R

V

R

V

I

Q

L

S

T

G
|
G

Q
x
Y

G
|
G

A

A

D
|
D

E
x
I

V

P

F

L

G

G

active site: A69 (vs. gap), G70 (vs. gap), D311 (≠ M344), Y337 (≠ Q370)
binding arginine-n-methylcarbonyl phosphoric acid 5'-adenosine ester: V236 (≠ L265), L237 (= L266), S238 (= S267), S243 (= S272), S261 (≠ T291), M262 (≠ V292), Y315 (≠ D348), L319 (≠ F352), G336 (= G369), Y337 (≠ Q370), G338 (= G371), D340 (= D373), I341 (≠ E374)
binding magnesium ion: D242 (= D271), D340 (= D373)
binding pyrophosphate 2-: S238 (= S267), G240 (= G269), D242 (= D271), S243 (= S272), D340 (= D373)

Sites not aligning to the query:

active site: 371, 432
binding arginine-n-methylcarbonyl phosphoric acid 5'-adenosine ester: 362, 371, 432, 434, 435
binding pyrophosphate 2-: 412, 432, 433

1mb9A Beta-lactam synthetase complexed with atp (see paper)
26% identity, 54% coverage: 60:378/595 of query aligns to 46:344/485 of 1mb9A

query
sites
1mb9A

H

H

Q

A

P

P

M

S

F

V

D

A

P

P

E

D

T

R

G

A

L

V

A

A

L

R

V

S

F

L

N

T

G

G

A

A

-

P

-

T

-

A

-

V

-

L

-
x
A

-
x
G

-

E

I

I

Y

Y

N

N

F

R

R

D

T

E

L

L

R

L

A

S

E

V

L

L

E

P

R

A

R

G

G

P

H

-

R

-

F

-

F

A

S

P

S

E

G

G

D

D

T

A

E

E

V

L

I

V

L

L

K

R

G

L

Y

L

A

E

E

R

W

Y

G

D

E

L

G

H

V

A

V

F

A

R

R

L

L

V

H

N

G

G

M

R

F

F

A

A

F

T

A

V

L

V

F

-

D

-

S

-

R

R

T

T

-

G

Q

D

K

R

I

V

L

L

A

A

R

T

D

D

R

H

M

A

G

G

I

S

K

V

P

P

L

L

Y

Y

L

T

A

C

R

V

S

A

G

P

H

G

G

E

I

V

R

R

F

A

A

S

S

T

N

E

T

A

Q

K

A

A

L

L

L

-

D

-

S

-

G

-

G

A

V

A

D

F

P

P

A

L

L

A

D

D

P

A

E

R

A

R

L

V

H

-

L

-

F

-

S

-

L

-

H

-

A

-

V

-

P

-

A

-

P

-

R

-

T

A

V

G

L

L

R

T

G

G

I

V

R

Y

K

Q

L

V

Q

P

P

A

G

G

H

A

Y

V

L

M

L

D

I

I

E

D

-

L

A

G

D

S

G

G

H

T

E

A

H

V

L

T

E

H

R

R

Y

T

W

W

Q

T

L

P

C

G

A

L

R

S

R

R

P

-

E

-

Q

R

A

I

I

L

D

P

D

E

D

G

E

E

W

A

R

V

E

A

R

A

I

V

E

R

A

A

G

A

L

L

R

E

R

K

A

A

V

V

R

A

R

Q

R

R

L

V

E

T

V

P

A

G

D

D

V

T

P

P

V

L

G

-

V

V

L
x
V

L
|
L

S
|
S

G

G

G
|
G

L

I

D
|
D

S
|
S

S

S

L

G

L

V

V

A

A

A

L

C

A

A

V

-

E

H

E

R

G

A

A

A

Q

G

D

E

L

L

R

D

T

T

F

V

T
x
S

V
x
M

G

G

F

-

E

-

D

-

Q

-

P

-

E

T

E

D

K

T

G

S

S

N

E

E

F

F

E

R

F

E

S

A

D

R

P

A

V

V

A

V

E

D

R

H

Y

L

A

R

T

T

D

R

H

H

Q

R

R

E

T

I

L

T

I

I

P

P

N

T

S

T

E

E

V

L

L

L

R

A

R

Q

L

L

P

P

E

Y

A

A

V

V

A

W

A

A

-

S

-

E

M

S

A

V

E

D

P

P

M
x
D

F

I

G

I

Q

E

D
x
Y

A

L

V

L

A

P

F
x
L

Y

T

L

A

L

L

A

Y

E

R

Q

A

V

L

S

D

Q

G

H

P

V

E

K

R

V

R

V

I

Q

L

S

T

G
|
G

Q
x
Y

G

G

A

A

D
|
D

E

I

V

P

F

L

G

G

active site: A70 (vs. gap), G71 (vs. gap), D310 (≠ M344), Y336 (≠ Q370)
binding adenosine monophosphate: V235 (≠ L265), L236 (= L266), S242 (= S272), S260 (≠ T291), M261 (≠ V292), Y314 (≠ D348), L318 (≠ F352), G335 (= G369), Y336 (≠ Q370)
binding adenosine-5'-triphosphate: V235 (≠ L265), L236 (= L266), S237 (= S267), G239 (= G269), D241 (= D271), S242 (= S272), S260 (≠ T291), M261 (≠ V292), L318 (≠ F352), G335 (= G369), D339 (= D373)
binding magnesium ion: D241 (= D271), D339 (= D373)
binding pyrophosphate 2-: S237 (= S267), G239 (= G269), D241 (= D271), S242 (= S272), D339 (= D373)

Sites not aligning to the query:

active site: 370, 431
binding adenosine-5'-triphosphate: 411, 431
binding pyrophosphate 2-: 411, 431

1mc1A Beta-lactam synthetase with product (dgpc), amp and ppi (see paper)
26% identity, 58% coverage: 32:378/595 of query aligns to 3:340/491 of 1mc1A

query
sites
1mc1A

P

P

D

A

A

A

D

F

G

G

V

F

W

L

A

A

S

S

G

A

R

R

A

G

A

P

L

V

G

F

H

A

R

T

R

R

L

G

S

S

I

H

L

T

D

D

L

I

-

D

-

T

-

P

-

Q

-

G

-

E

-

R

-

S

-

L

-

A

S

A

Q

T

R

L

A

V

H

H

Q

A

P

P

M

S

F

V

D

A

P

P

E

D

T

R

G

A

L

V

A

A

L

R

V

S

F

L

N

T

G

G

A

A

-

P

-

T

-

A

-

V

-

L

-
x
A

-
x
G

-

E

I

I

Y

Y

N

N

F

R

R

D

T

E

L

L

R

L

A

S

E

V

L

L

E

P

R

A

R

G

G

P

H

-

R

-

F

-

F

A

S

P

S

E

G

G

D

D

T

A

E

E

V

L

I

V

L

L

K

R

G

L

Y

L

A

E

E

R

W

Y

G

D

E

L

G

H

V

A

V

F

A

R

R

L

L

V

H

N

G

G

M

R

F

F

A

A

F

T

A

V

L

V

F

-

D

-

S

-

R

R

T

T

-

G

Q

D

K

R

I

V

L

L

A

A

R

T

D

D

R

H

M

A

G

G

I

S

K

V

P

P

L

L

Y

Y

L

T

A

C

R

V

S

A

G

P

H

G

G

E

I

V

R

R

F

A

A

S

S

T

N

E

T

A

Q

K

A

A

L

L

L

-

D

-

S

A

G

A

G

G

V

F

D

-

P

P

A

L

L

A

D

D

P

A

E

R

A

R

L

V

H

-

L

-

F

-

S

-

L

-

H

-

A

-

V

-

P

-

A

-

P

-

R

-

T

A

V

G

L

L

R

T

G

G

I

V

R

Y

K

Q

L

V

Q

P

P

A

G

G

H

A

Y

V

L

M

L

D

I

I

E

D

-

L

A

G

D

S

G

G

H

T

E

A

H

V

L

T

E

H

R

R

Y

T

W

W

Q

T

L

P

C

G

A

L

R

S

R

R

P

-

E

-

Q

R

A

I

I

L

D

P

D

E

D

G

E

E

W

A

R

V

E

A

R

A

I

V

E

R

A

A

G

A

L

L

R

E

R

K

A

A

V

V

R

A

R

Q

R

R

L

V

E

T

V

P

A

G

D

D

V

T

P

P

V

L

G

-

V

V

L
x
V

L

L

S
|
S

G

G

G
|
G

L

I

D
|
D

S
|
S

S

S

L

G

L

V

V

A

A

A

L

C

A

A

V

-

E

H

E

R

G

A

A

A

Q

G

D

E

L

L

R

D

T

T

F

V

T
x
S

V
x
M

G

G

F

-

E

-

D

-

Q

-

P

-

E

T

E

D

K

T

G

S

S

N

E

E

F

F

E

R

F

E

S

A

D

R

P

A

V

V

A

V

E

D

R

H

Y

L

A

R

T

T

D

R

H

H

Q

R

R

E

T

I

L

T

I

I

P

P

N

T

S

T

E

E

V

L

L

L

R

A

R

Q

L

L

P

P

E

Y

A

A

V

V

A

W

A

A

-

S

-

E

M

S

A

V

E

D

P

P

M
x
D

F

I

G

I

Q

E

D
x
Y

A

L

V

L

A

P

F

L

Y

T

L

A

L

L

A

Y

E

R

Q

A

V

L

S

D

Q

G

H

P

V

E

K

R

V

R

V

I

Q

L

S

T

G
|
G

Q
x
Y

G
|
G

A

A

D
|
D

E
x
I

V

P

F

L

G

G

active site: A65 (vs. gap), G66 (vs. gap), D306 (≠ M344), Y332 (≠ Q370)
binding adenosine monophosphate: V231 (≠ L265), S233 (= S267), S238 (= S272), S256 (≠ T291), M257 (≠ V292), G331 (= G369)
binding magnesium ion: D237 (= D271), D335 (= D373)
binding deoxyguanidinoproclavaminic acid: Y310 (≠ D348), Y332 (≠ Q370), G333 (= G371), I336 (≠ E374)
binding pyrophosphate 2-: S233 (= S267), G235 (= G269), D237 (= D271), S238 (= S272), D335 (= D373)

Sites not aligning to the query:

active site: 366, 427
binding adenosine monophosphate: 427, 430
binding deoxyguanidinoproclavaminic acid: 357, 366, 427
binding pyrophosphate 2-: 407, 427, 428

Q9STG9 Amidophosphoribosyltransferase 2, chloroplastic; AtATase2; AtPURF2; PRPP2; Glutamine phosphoribosylpyrophosphate amidotransferase 2; AtGPRAT2; Protein CHLOROPLAST IMPORT APPARATUS 1; Protein DIFFERENTIAL DEVELOPMENT OF VASCULAR ASSOCIATED CELLS; EC 2.4.2.14 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
31% identity, 21% coverage: 41:165/595 of query aligns to 151:285/561 of Q9STG9

query
sites
Q9STG9

G

G

R

D

A

I

A

A

L

I

G

G

H

H

R

V

R

R

L

Y

S

S

I

T

L

A

D

G

L

S

S

S

Q

M

-

L

R

K

A

N

H

V

Q

Q

P

P

M

F

F

V

D

A

P

G

E

Y

-

R

-

F

T

G

G

S

L

V

A

G

L

V

V

A

F
x
H

N

N

G

G

A

N

I

L

Y

V

N

N

F

Y

R

T

T

K

L

L

R

R

A

A

E

D

L

L

E

E

R

E

R

N

G

G

H

S

R

I

F

F

F

N

S

T

S

S

G

S

D

D

T

T

E

E

V

V

I

V

L

L

K

H

G

L

Y

I

A

A

E

I

W

S

G

K

E

A

-

R

-

P

-

F

-

M

-

R

-

I

-

V

G

D

V

A

V

C

A

E

R

K

L

L

H

Q

G

G

M

-

F

-

A

A

F

Y

A

S

L

M

F

V

D

F

S

V

R

T

T

E

Q

D

K

K

I

L

L

V

L

A

A

V

R

R

D

D

R

P

M

H

G

G

I

F

K
x
R

P
|
P

L

L

Y

V

L

M

A

G

R

R

S

R

G

S

H

N

G

G

-

A

I

V

R

V

F

F

A

A

S

S

N

E

T

T

Q

C

A

A

L

L

H187 (≠ F74) mutation to T: In cia1-2; small plants with white leaves showing an irregular mosaic of green sectors.
R264 (≠ K145) mutation to K: Strong resistance to the bleaching herbicides DAS073 and DAS734.
P265 (= P146) mutation to S: Low resistance to the bleaching herbicides DAS073 and DAS734; when associated with F-494.

Sites not aligning to the query:

371 G→S: Low resistance to the bleaching herbicides DAS073 and DAS734.
476 P→S: Resistance to the bleaching herbicides DAS073 and DAS734.
494 Y→F: Low resistance to the bleaching herbicides DAS073 and DAS734; when associated with S-265.

6lbpA Structure of the glutamine phosphoribosylpyrophosphate amidotransferase from arabidopsis thaliana (see paper)
31% identity, 21% coverage: 41:165/595 of query aligns to 65:199/460 of 6lbpA

query
sites
6lbpA

G

G

R

D

A

I

A

A

L

I

G

G

H

H

R

V

R

R

L

Y

S

S

I

T

L

A

D

G

L

S

S

S

Q

M

-

L

R

K

A

N

H

V

Q

Q

P

P

M

F

F

V

D

A

P

G

E

Y

-

R

-

F

T

G

G

S

L

V

A

G

L

V

V

A

F

H

N
|
N

G
|
G

A

N

I

L

Y

V

N

N

F

Y

R

T

T

K

L

L

R

R

A

A

E

D

L

L

E

E

R

E

R

N

G

G

H

S

R

I

F

F

F

N

S

T

S

S

G

S

D

D

T

T

E

E

V

V

I

V

L

L

K

H

G

L

Y

I

A

A

E

I

W

S

G

K

E

A

-

R

-

P

-

F

-

M

-

R

-

I

-

V

G

D

V

A

V

C

A

E

R

K

L

L

H

Q

G

G

M

-

F

-

A

A

F

Y

A

S

L

M

F

V

D

F

S

V

R

T

T

E

Q

D

K

K

I

L

L

V

L

A

A

V

R

R

D

D

R

P

M

H

G

G

I

F

K

R

P

P

L

L

Y

V

L

M

A

G

R

R

S

R

G

S

H

N

G

G

-

A

I

V

R

V

F

F

A

A

S

S

N

E

T

T

Q

C

A

A

L

L

active site: N102 (= N75), G103 (= G76)

Sites not aligning to the query:

active site: 1, 27, 243, 301, 306, 316, 424
binding iron/sulfur cluster: 237, 239, 383, 385, 434, 436, 437

1ao0A Glutamine phosphoribosylpyrophosphate (prpp) amidotransferase from b. Subtilis complexed with adp and gmp (see paper)
24% identity, 47% coverage: 2:282/595 of query aligns to 1:289/455 of 1ao0A

query
sites
1ao0A

C
|
C

G

G

I

V

A

F

G

G

E

-

F

-

V

I

W

W

G

G

D

H

R

E

A

A

D

P

V

-

D

Q

A

I

V

T

A

Y

R

Y

M

G

L

L

P

H

A

S

L

L

A

Q

R
x
H

R

R

G
|
G

P

Q

D

E

A

G

D

A

G

G

V

I

W

V

A

A

S

T

-

D

-

G

-

E

-

K

-

L

-

T

-

A

-

H

-

K

-

G

-

Q

-

G

-

L

-

I

-

T

-

E

-

V

-

F

-

Q

-

N

-

G

-

E

-

L

-

S

-

K

-

V

-

K

G

G

R

K

A

G

A

A

L

I

G

G

H

H

R

V

R

R

L

Y

S

A

I

T

L

-

D

-

L

-

S

G

Q

Y

R

E

A

N

H

V

Q

Q

P

P

M

L

F

L

-

F

-

R

-

S

D

Q

P

N

E

N

T

G

G

S

L

L

A

A

L

L

V

A

F

H

N
|
N

G
|
G

A

N

I

L

Y

V

N

N

F

A

R

T

T

Q

L

L

R

K

A

Q

E

Q

L

L

E

E

R

N

R

Q

G

G

H

S

R

I

F

F

F

Q

S

T

S

S

G

S

D

D

T

T

E

E

V

V

I

L

L

A

K

H

G

L

Y

I

A

K

E

R

W

S

G

G

-

H

-

F

-

T

-

L

-

K

-

D

-

Q

-

I

E

K

G

N

V

S

V

L

A

S

R

M

L

L

H

K

G

G

M

A

F

Y

A

A

F

F

A

L

L

I

F

M

D

T

S

E

R

-

T

-

Q

T

K

E

I

M

L

I

L

V

A

A

R

L

D

D

R

P

M

N

G

G

I

L

K

R

P

P

L

L

Y

S

L

I

A

G

R

M

S

M

G

G

H

D

G

A

I

Y

R

V

F

V

A

A

S

S

N

E

T

T

Q

C

A

A

L

F

L

-

D

-

S

-

G

-

V

-

D

-

P

-

A

-

L

-

D

-

P

-

E

-

A

-

L

-

H

-

L

-

F

-

S

-

L

-

H

-

A

D

V

V

P

G

A

A

P

T

R

Y

T

-

V

-

L

-

R

-

G

-

I

L

R

R

K

E

L

V

Q

E

P

P

G

G

H

E

Y

M

L

L

L

I

I

I

E

N

A

D

D

E

G

G

H

M

E

K

H

S

L

-

E

E

R

R

Y

F

W

S

-

M

-

N

-

I

-

N

-

R

Q

S

L

I

C
|
C

A
x
S

-
x
M

-

E

-

Y

-

I

-
x
Y

-

F

R
x
S

R
|
R

P
|
P

E

D

Q

S

A

N

I

I

D

D

D

G

-

I

D

N

E

V

W

H

R

S

E

A

R

R

I

K

E

N

A

L

G

G

L

K

R

M

R

L

A

A

V

Q

R

E

R

S

R

A

L

V

E

E

V

-

A

A

D

D

V

V

P

V

V

T

G

G

V

V

L

-

S

-

G

-

L
x
P

D
|
D

S
|
S

S

S

L

I

L

S

V

A

A

A

L

I

A

G

V

Y

E

A

E

E

active site: C1 (= C2), G27 (= G31), N98 (= N75), G99 (= G76), Y238 (vs. gap)
binding guanosine-5'-monophosphate: M234 (vs. gap), Y238 (vs. gap), S279 (= S272)
binding adenosine-5'-diphosphate: H25 (≠ R29), Y238 (vs. gap), S240 (≠ R228), R241 (= R229), P242 (= P230), P277 (≠ L270), D278 (= D271), S279 (= S272)
binding magnesium ion: S279 (= S272)
binding iron/sulfur cluster: C232 (= C226), S233 (≠ A227), M234 (vs. gap)

Sites not aligning to the query:

active site: 296, 301, 311, 419
binding guanosine-5'-monophosphate: 341, 342, 343, 345, 346, 347, 348, 349
binding adenosine-5'-diphosphate: 300, 301, 301, 303
binding magnesium ion: 341, 342
binding iron/sulfur cluster: 378, 380, 433, 436

P14742 Glutamine--fructose-6-phosphate aminotransferase [isomerizing]; GFAT; D-fructose-6-phosphate amidotransferase; Hexosephosphate aminotransferase; EC 2.6.1.16 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
30% identity, 15% coverage: 17:108/595 of query aligns to 56:148/717 of P14742

query
sites
P14742

V

I

D

G

A

K

V

V

A

S

R

A

M

L

L

K

P

E

A

E

L

I

A

T

R

K

R

Q

G

N

P

P

D

N

A

R

D

D

G

V

V

T

W

F

A

V

S

S

G

-

R

H

A

C

A

G

L

I

G

A

H

H

R

T

R

R

L

W

S

A

I

T

L

H

D

G

L

R

S

P

Q

E

R

Q

-

V

-

N

A

C

H

H

Q

P

P

Q

M

R

F

S

D

D

P

P

E

E

T

D

G

Q

L

F

A

V

L

V

V

V

F

H

N

N

G

G

A

I

I

I

Y

T

N

N

F

F

R

R

T

E

L

L

R

K

A

T

E

L

L

L

E

I

R

N

R

K

G

G

H

Y

R

K

F

F

F

E

S

S

S

D

G

T

D

D

T

T

E

E

V

C

I

I

L

A

K

K

G

L

Y

Y

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 active site, For GATase activity

Query Sequence

>WP_006748146.1 NCBI__GCF_000227685.2:WP_006748146.1
MCGIAGEFVWGDRRADVDAVARMLPALARRGPDADGVWASGRAALGHRRLSILDLSQRAH
QPMFDPETGLALVFNGAIYNFRTLRAELERRGHRFFSSGDTEVILKGYAEWGEGVVARLH
GMFAFALFDSRTQKILLARDRMGIKPLYLARSGHGIRFASNTQALLDSGGVDPALDPEAL
HLLFSLHAVVPAPRTVLRGIRKLQPGHYLLIEADGHEHLERYWQLCARRPEQAIDDDEWR
ERIEAGLRRAVRRRLEVADVPVGVLLSGGLDSSLLVALAVEEGAQDLRTFTVGFEDQPEE
KGSEFEFSDPVAERYATDHQRTLIPNSEVLRRLPEAVAAMAEPMFGQDAVAFYLLAEQVS
QHVKVVQSGQGADEVFGGYFWYPKMAEERSGDWAERFRKHYFDRDDPEMRELLAAPPPND
LAGEWLRAELAMLETDRADSFIDAVLALDVTTLIVDDPVKRVDNMTMAFGLEARVPFLDH
ELVETAAAMPPHLKLGDGGKKVLKQIARGRLPDAVLDRPKGYFPVPALKYVRGDFLEFMR
DILGSQAARERGIYRPEYVQRLLDAPDQYFTRIQGSKLWHLALLEFWLQTHVDRA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory