SitesBLAST
Comparing WP_007152784.1 NCBI__GCF_000170835.1:WP_007152784.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
P42412 Malonate-semialdehyde dehydrogenase; MSA dehydrogenase; Methylmalonate-semialdehyde dehydrogenase; MMSA dehydrogenase; MMSDH; MSDH; EC 1.2.1.27 from Bacillus subtilis (strain 168) (see 3 papers)
47% identity, 98% coverage: 1:484/496 of query aligns to 4:485/487 of P42412
- C36 (≠ A33) mutation to A: No effect at either the structural or enzymatic levels; when associated with A-160; A-287; A-351 and A-413.
- R107 (= R104) mutation to L: At least 50-fold decrease of the second-order rate constant for the acylation step.
- A150 (≠ T147) binding NAD(+)
- F152 (= F149) binding NAD(+)
- C160 (≠ L157) mutation to A: No effect at either the structural or enzymatic levels; when associated with A-36; A-287; A-351 and A-413.
- K176 (= K173) binding NAD(+)
- E179 (= E176) binding NAD(+)
- R180 (≠ Q177) binding NAD(+)
- S229 (= S226) binding NAD(+)
- T251 (≠ A248) binding NAD(+)
- R283 (= R280) mutation to L: At least 50-fold decrease of the second-order rate constant for the acylation step.
- C287 (≠ I284) mutation to A: No effect at either the structural or enzymatic levels; when associated with A-36; A-160; A-351 and A-413.
- C351 (≠ L347) mutation to A: No effect at either the structural or enzymatic levels; when associated with A-36; A-160; A-287 and A-413.
- E382 (= E381) binding NAD(+)
- C413 (≠ S412) mutation to A: No effect at either the structural or enzymatic levels; when associated with A-36; A-160; A-287 and A-351.
1t90A Crystal structure of methylmalonate semialdehyde dehydrogenase from bacillus subtilis
47% identity, 98% coverage: 1:484/496 of query aligns to 2:483/484 of 1t90A
- active site: N151 (= N150), K174 (= K173), L248 (≠ F247), C282 (= C281), E380 (= E381), A460 (= A461)
- binding nicotinamide-adenine-dinucleotide: I147 (= I146), A148 (≠ T147), P149 (= P148), F150 (= F149), N151 (= N150), W159 (= W158), K174 (= K173), E177 (= E176), R178 (≠ Q177), H207 (≠ K206), V225 (= V224), G226 (= G225), S227 (= S226), V230 (= V229), L248 (≠ F247), T249 (≠ A248), C282 (= C281), E380 (= E381), F382 (= F383)
4zz7A Crystal structure of methylmalonate-semialdehyde dehydrogenase (dddc) from oceanimonas doudoroffii (see paper)
47% identity, 97% coverage: 1:483/496 of query aligns to 1:484/489 of 4zz7A
- active site: N149 (= N150), K172 (= K173), L246 (≠ F247), C280 (= C281), E382 (= E381), A462 (= A461)
- binding nicotinamide-adenine-dinucleotide: T146 (= T147), P147 (= P148), F148 (= F149), N149 (= N150), K172 (= K173), E175 (= E176), K205 (= K206), V208 (= V209), F222 (= F223), V223 (= V224), G224 (= G225), S225 (= S226), I228 (≠ V229), L246 (≠ F247), G247 (≠ A248), C280 (= C281), E382 (= E381), F384 (= F383)
4iymC Crystal structure of putative methylmalonate-semialdehyde dehydrogenase from sinorhizobium meliloti 1021 complexed with NAD, target 011934
50% identity, 96% coverage: 7:482/496 of query aligns to 10:487/491 of 4iymC
- active site: N153 (= N150), K176 (= K173), F250 (= F247), C284 (= C281), E386 (= E381), Q466 (≠ A461)
- binding nicotinamide-adenine-dinucleotide: I149 (= I146), T150 (= T147), P151 (= P148), F152 (= F149), N153 (= N150), F154 (= F151), K176 (= K173), K209 (= K206), V212 (= V209), F226 (= F223), V227 (= V224), G228 (= G225), S229 (= S226), I232 (≠ V229), G251 (≠ A248), C284 (= C281), E386 (= E381), F388 (= F383)
5tjrD X-ray crystal structure of a methylmalonate semialdehyde dehydrogenase from pseudomonas sp. Aac (see paper)
49% identity, 95% coverage: 8:480/496 of query aligns to 7:455/468 of 5tjrD
- active site: N144 (= N150), K167 (= K173), L241 (≠ F247), C270 (= C281), E356 (= E381), A436 (= A461)
- binding adenosine-5'-diphosphate: I140 (= I146), T141 (= T147), F143 (= F149), K167 (= K173), E170 (= E176), K200 (= K206), F217 (= F223), S220 (= S226), I223 (≠ V229)
4cazA Crystal structure of betaine aldehyde dehydrogenase from pseudomonas aeruginosa in complex with nadh
32% identity, 95% coverage: 6:476/496 of query aligns to 8:478/489 of 4cazA
- active site: N152 (= N150), K175 (= K173), E251 (≠ F247), C285 (= C281), E386 (= E381), E463 (≠ Q459)
- binding [[(2R,3S,4R,5R)-5-[(3R)-3-aminocarbonyl-3,4-dihydro-2H-pyridin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanidyl-phosphoryl] [(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methyl phosphate: I148 (= I146), G149 (≠ T147), W151 (≠ F149), N152 (= N150), K175 (= K173), E178 (= E176), G208 (≠ K206), G212 (≠ D210), F226 (= F223), T227 (≠ V224), G228 (= G225), G229 (≠ S226), T232 (≠ V229), V236 (≠ I233), E251 (≠ F247), L252 (≠ A248), C285 (= C281), E386 (= E381), F388 (= F383)
2woxA Betaine aldehyde dehydrogenase from pseudomonas aeruginosa with NAD(p) h-catalytic thiol adduct. (see paper)
32% identity, 95% coverage: 6:476/496 of query aligns to 8:478/489 of 2woxA
- active site: N152 (= N150), K175 (= K173), E251 (≠ F247), C285 (= C281), E386 (= E381), E463 (≠ Q459)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: I148 (= I146), G149 (≠ T147), W151 (≠ F149), N152 (= N150), K175 (= K173), S177 (= S175), E178 (= E176), G208 (≠ K206), G212 (≠ D210), F226 (= F223), T227 (≠ V224), G228 (= G225), G229 (≠ S226), T232 (≠ V229), V236 (≠ I233), E251 (≠ F247), L252 (≠ A248), C285 (= C281), E386 (= E381), F388 (= F383)
2wmeA Crystallographic structure of betaine aldehyde dehydrogenase from pseudomonas aeruginosa (see paper)
32% identity, 95% coverage: 6:476/496 of query aligns to 8:478/489 of 2wmeA
- active site: N152 (= N150), K175 (= K173), E251 (≠ F247), C285 (= C281), E386 (= E381), E463 (≠ Q459)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G149 (≠ T147), W151 (≠ F149), K175 (= K173), S177 (= S175), E178 (= E176), G208 (≠ K206), G212 (≠ D210), F226 (= F223), G228 (= G225), G229 (≠ S226), T232 (≠ V229), V236 (≠ I233)
Q9HTJ1 NAD/NADP-dependent betaine aldehyde dehydrogenase; BADH; EC 1.2.1.8 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see 2 papers)
32% identity, 95% coverage: 6:476/496 of query aligns to 9:479/490 of Q9HTJ1
- GAWN 150:153 (≠ TPFN 147:150) binding NADPH
- K162 (≠ M159) active site, Charge relay system
- KPSE 176:179 (= KPSE 173:176) binding NADPH
- G209 (≠ K206) binding NADPH
- GTST 230:233 (≠ SVPV 226:229) binding NADPH
- E252 (≠ F247) active site, Proton acceptor
- C286 (= C281) binding covalent; modified: Cysteine sulfenic acid (-SOH)
- E387 (= E381) binding NADPH
- E464 (≠ Q459) active site, Charge relay system
6wsbA Crystal structure of a betaine aldehyde dehydrogenase from burkholderia pseudomallei bound to cofactor NAD (see paper)
33% identity, 95% coverage: 6:476/496 of query aligns to 8:477/489 of 6wsbA