SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_007472841.1 NCBI__GCF_000170735.1:WP_007472841.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q04797 Aspartate-semialdehyde dehydrogenase; ASA dehydrogenase; ASADH; Aspartate-beta-semialdehyde dehydrogenase; EC 1.2.1.11 from Bacillus subtilis (strain 168) (see paper)
50% identity, 95% coverage: 3:333/350 of query aligns to 6:337/346 of Q04797

query
sites
Q04797

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S98 (= S97) modified: Phosphoserine
Y146 (≠ F147) modified: Phosphotyrosine

P23247 Aspartate-semialdehyde dehydrogenase 2; ASA dehydrogenase 2; ASADH 2; Aspartate-beta-semialdehyde dehydrogenase 2; EC 1.2.1.11 from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) (see paper)
50% identity, 95% coverage: 2:333/350 of query aligns to 5:329/337 of P23247

query
sites
P23247

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C132 (= C131) active site, Acyl-thioester intermediate

2r00C Crystal structure of aspartate semialdehyde dehydrogenase ii complexed with asa from vibrio cholerae (see paper)
50% identity, 95% coverage: 2:333/350 of query aligns to 4:328/336 of 2r00C

query
sites
2r00C

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active site: C131 (= C131), Q158 (= Q158), R237 (= R240), H244 (= H247)
binding 2,2'-oxydiacetic acid: V15 (= V13), N95 (= N95), C131 (= C131), N315 (≠ Q320)

8jusA Crystal structure of aspartate semialdehyde dehydrogenase from porphyromonas gingivalis complexed with 2',5'adenosine diphosphate
49% identity, 94% coverage: 4:333/350 of query aligns to 3:326/335 of 8jusA

query
sites
8jusA

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I

C

D

D

N

E

P

P

E

S

K

E

N

K

E

Q

Y

Y

P

P

M

M

P

P

I

L

I

F

A

G

T

T

D

E

T

Q

D

D

Y

P

V

V

Y

I

V

V

G

G

R

R

I

I

R

R

K

Q

D

D

L

L

F

A

D

N

N

P

K

N

T

G

L

L

H

V

F

F

F

W

N

C

V

V

A

S

D

D

Q

Q

L

I

R

R

V

K

G

G

A

A

T

L

N

N

A

A

V

V

R

Q

I

I

A

A

binding adenosine-2'-5'-diphosphate: G7 (= G8), S9 (≠ T10), G10 (= G11), G34 (≠ A35), S35 (= S36), R37 (= R38), S38 (= S39), S70 (= S72), A71 (= A73), T75 (≠ V77)

3pylC Crystal structure of aspartate beta-semialdehide dehydrogenase from streptococcus pneumoniae with d-2,3-diaminopropionate (see paper)
46% identity, 95% coverage: 2:333/350 of query aligns to 2:337/361 of 3pylC

query
sites
3pylC

Y

Y

N

T

V

V

A

A

I

V

V

V

G

G

A

A

T

T

G

G

A

A

V

V

G

G

E

A

E

Q

L

M

F

I

R

K

V

M

L

L

E

E

Y

S

N

T

F

L

P

P

V

I

K

D

K

K

L

I

V

R

P

Y

L

L

A

A

S

S

A

A

R

R

S

S

V

A

G

G

K

K

E

S

V

L

E

K

F

F

K

K

G

D

E

Q

T

D

L

I

K

T

V

I

K

E

E

E

L

T

T

T

C

E

D

T

V

A

F

F

E

E

K

G

E

-

I

V

D

D

I

I

A

A

F

L

F

F

S

S

A

A

G

G

G

S

S

S

V

T

S

S

A

A

K

K

F

Y

A

A

P

P

C

Y

A

A

V

V

K

K

A

A

G

G

A

V

V

V

I

V

D

D

N

N

T

T

S

S

Y

Y

F

F

R

R

M

Q

D

N

K

P

D

D

V

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

E

H

D

-

I

-

A

A

L

L

W

D

R

A

K

H

K

N

G

G

I

I

A

A

N

C

P

P

N
|
N

C
|
C

S

S

T

T

I

I

Q

Q

M

M

V

M

I

V

S

A

L

L

A

E

P

P

L

V

H

R

K

Q

E

K

F

W

N

G

I

L

K

D

R

R

V

I

E

I

V

V

A

S

T

T

Y

Y

Q

Q

A

A

V

V

S

S

G
|
G

A

A

G

G

K

M

K

G

G

A

M

I

E

L

E

E

L

T

F

Q

E

R

Q

E

M

L

R

R

A

E

I

V

F

L

N

N

F

D

K

G

L

V

D

K

K

P

V

C

V

D

D

L

E

H

R

A

E

E

V

I

F

L

-

P

-

S

-

G

-

D

-

K

Q

H

Y

Y

Q

P

I

I

A

A

L

F

N

N

V

A

I

L

P

P

H

Q

I

I

D

D

R

V

F

F

M

T

D

D

N

N

G

D

F

Y

T

T

R

Y

E
|
E

E

E

M

M

K

K

M

M

V

T

N

K

E

E

T

T

N

K

K

K

I

I

M

M

H

E

S

D

N

D

-

S

I

I

E

A

V

V

A

S

P

A

T

T

C

C

V

V

R
|
R

V

I

P

P

V

V

L

L

R

S

S

A

H

H

S

S

E

E

A

S

I

V

T

Y

I

I

H

E

C

T

E

K

K

E

E

V

V

A

D

P

V

I

K

E

R

E

A

V

R

K

E

A

V

A

L

I

E

A

S

A

F

F

E

P

D

G

I

A

K

V

V

L

I

E

D

D

N

D

P

V

E

A

K

H

N

Q

E

I

Y

Y

P

P

M

Q

P

A

I

I

I

N

A

A

T

V

D

G

T

S

D

R

Y

D

V

T

Y

F

V

V

G

G

R

R

I

I

R

R

K

K

D

D

L

L

F

D

D

A

N

E

K

K

T

G

L

I

H

H

F

M

F

W

N

V

V

V

A

S

D

D

Q

N

L

L

R

L

V

K

G

G

A

A

T

W

N

N

A

S

V

V

R

Q

I

I

A

A

binding 3-amino-D-alanine: N126 (= N130), C127 (= C131), G158 (= G162), E219 (= E216), R244 (= R240)

4r51A Crystal complex structure of sp-aspartate-semialdehyde-dehydrogenase with nicotinamide adenine dinucleotide phosphate and phthalic acid (see paper)
46% identity, 95% coverage: 2:333/350 of query aligns to 2:337/360 of 4r51A

query
sites
4r51A

Y

Y

N

T

V

V

A

A

I

V

V

V

G
|
G

A

A

T
|
T

G
|
G

A
|
A

V
|
V

G

G

E

A

E

Q

L

M

F

I

R

K

V

M

L

L

E

E

Y

S

N

T

F

L

P

P

V

I

K

D

K

K

L

I

V

R

P

Y

L

L

A
|
A

S
|
S

A

A

R

R

S
|
S

V

A

G

G

K

K

E

S

V

L

E

K

F

F

K

K

G

D

E

Q

T

D

L

I

K

T

V

I

K

E

E

E

L
x
T

T

T

C

E

D

T

V

A

F

F

E

E

K

G

E

-

I

V

D

D

I

I

A

A

F

L

F

F

S
|
S

A
|
A

G
|
G

G
x
S

S

S

V

T

S

S

A

A

K

K

F

Y

A

A

P

P

C

Y

A

A

V

V

K

K

A

A

G

G

A

V

V

V

I

V

D

D

N
|
N

T
|
T

S
|
S

Y

Y

F

F

R
|
R

M

Q

D

N

K

P

D

D

V

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

E

H

D

-

I

-

A

A

L

L

W

D

R

A

K

H

K

N

G

G

I

I

A

A

N

C

P

P

N
|
N

C
|
C

S

S

T

T

I

I

Q

Q

M

M

V

M

I

V

S

A

L

L

A

E

P

P

L

V

H

R

K

Q

E

K

F

W

N

G

I

L

K

D

R

R

V

I

E

I

V

V

A

S

T

T

Y

Y

Q

Q

A

A

V

V

S

S

G

G

A

A

G
|
G

K
x
M

K

G

G

A

M

I

E

L

E

E

L

T

F

Q

E

R

Q

E

M

L

R

R

A

E

I

V

F

L

N

N

F

D

K

G

L

V

D

K

K

P

V

C

V

D

D

L

E

H

R

A

E

E

V

I

F

L

-

P

-

S

-

G

-

D

-

K

Q

H

Y

Y

Q

P

I

I

A

A

L

F

N

N

V

A

I

L

P

P

H

Q

I

I

D

D

R

V

F

F

M

T

D

D

N

N

G

D

F

Y

T

T

R

Y

E

E

M

M

K
|
K

M

M

V

T

N

K

E

E

T

T

N

K

K

K

I

I

M

M

H

E

S

D

N

D

-

S

I

I

E

A

V

V

A

S

P

A

T

T

C

C

V

V

R

R

V

I

P

P

V

V

L

L

R

S

S

A

H

H

S

S

E

E

A

S

I

V

T

Y

I

I

H

E

C

T

E

K

K

E

E

V

V

A

D

P

V

I

K

E

R

E

A

V

R

K

E

A

V

A

L

I

E

A

S

A

F

F

E

P

D

G

I

A

K

V

V

L

I

E

D

D

N

D

P

V

E

A

K

H

N

Q

E

I

Y

Y

P

P

M

Q

P

A

I

I

I

N

A

A

T

V

D

G

T

S

D

R

Y

D

V

T

Y

F

V

V

G

G

R

R

I

I

R

R

K

K

D

D

L

L

F

D

D

A

N

E

K

K

T

G

L

I

H

H

F

M

F

W

N

V

V

V

A

S

D

D

Q

N

L

L

R

L

V

K

G
|
G

A

A

T

W

N

N

A

S

V

V

R

Q

I

I

A

A

binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G8), T10 (= T10), G11 (= G11), A12 (= A12), V13 (= V13), A35 (= A35), S36 (= S36), S39 (= S39), T56 (≠ L56), S70 (= S72), A71 (= A73), G72 (= G74), N93 (= N95), T94 (= T96), N126 (= N130), C127 (= C131), G160 (= G164), M161 (≠ K165), G328 (= G324)
binding phthalic acid: S73 (≠ G75), T94 (= T96), S95 (= S97), R98 (= R100), N126 (= N130), K222 (= K219)

4r54A Complex crystal structure of sp-aspartate-semialdehyde-dehydrogenase with 3-carboxy-ethyl-phthalic acid (see paper)
46% identity, 95% coverage: 2:333/350 of query aligns to 2:337/357 of 4r54A

query
sites
4r54A

Y

Y

N

T

V

V

A

A

I

V

V

V

G
|
G

A

A

T
|
T

G
|
G

A
|
A

V
|
V

G

G

E

A

E

Q

L

M

F

I

R

K

V

M

L

L

E

E

Y

S

N

T

F

L

P

P

V

I

K

D

K

K

L

I

V

R

P

Y

L

L

A
|
A

S
|
S

A

A

R
|
R

S
|
S

V

A

G

G

K

K

E

S

V

L

E

K

F

F

K

K

G

D

E

Q

T

D

L

I

K

T

V

I

K

E

E

E

L
x
T

T

T

C

E

D

T

V

A

F

F

E

E

K

G

E

-

I

V

D

D

I

I

A

A

F

L

F

F

S
|
S

A
|
A

G
|
G

G
x
S

S

S

V
x
T

S

S

A

A

K

K

F

Y

A

A

P

P

C

Y

A

A

V

V

K

K

A

A

G

G

A

V

V

V

I

V

D

D

N
|
N

T
|
T

S
|
S

Y

Y

F

F

R
|
R

M

Q

D

N

K

P

D

D

V

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

E

H

D

-

I

-

A

A

L

L

W

D

R

A

K

H

K

N

G

G

I

I

A

A

N

C

P

P

N
|
N

C
|
C

S

S

T

T

I

I

Q

Q

M

M

V

M

I

V

S

A

L

L

A

E

P

P

L

V

H

R

K

Q

E

K

F

W

N

G

I

L

K

D

R

R

V

I

E

I

V

V

A

S

T

T

Y

Y

Q

Q

A

A

V

V

S

S

G

G

A

A

G
|
G

K

M

K

G

G

A

M

I

E

L

E

E

L

T

F

Q

E

R

Q

E

M

L

R

R

A

E

I

V

F

L

N

N

F

D

K

G

L

V

D

K

K

P

V

C

V

D

D

L

E

H

R

A

E

E

V

I

F

L

-

P

-

S

-

G

-

D

-

K

Q

H

Y

Y

Q

P

I

I

A

A

L

F

N

N

V

A

I

L

P

P

H

Q

I

I

D

D

R

V

F

F

M

T

D

D

N

N

G

D

F

Y

T

T

R

Y

E

E

M

M

K
|
K

M

M

V

T

N

K

E

E

T

T

N

K

K

K

I

I

M

M

H

E

S

D

N

D

-

S

I

I

E

A

V

V

A

S

P

A

T

T

C

C

V

V

R

R

V

I

P

P

V

V

L

L

R

S

S

A

H

H

S

S

E

E

A

S

I

V

T

Y

I

I

H

E

C

T

E

K

K

E

E

V

V

A

D

P

V

I

K

E

R

E

A

V

R

K

E

A

V

A

L

I

E

A

S

A

F

F

E

P

D

G

I

A

K

V

V

L

I

E

D

D

N

D

P

V

E

A

K

H

N

Q

E

I

Y

Y

P

P

M

Q

P

A

I

I

I

N

A

A

T

V

D

G

T

S

D

R

Y

D

V

T

Y

F

V

V

G

G

R

R

I

I

R

R

K

K

D

D

L

L

F

D

D

A

N

E

K

K

T

G

L

I

H

H

F

M

F

W

N

V

V

V

A

S

D

D

Q

N

L

L

R

L

V

K

G
|
G

A

A

T

W

N

N

A

S

V

V

R

Q

I

I

A

A

binding 3-(2-carboxyethyl)benzene-1,2-dicarboxylic acid: G72 (= G74), S73 (≠ G75), T94 (= T96), S95 (= S97), R98 (= R100), K222 (= K219)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G8), T10 (= T10), G11 (= G11), A12 (= A12), V13 (= V13), A35 (= A35), S36 (= S36), R38 (= R38), S39 (= S39), T56 (≠ L56), S70 (= S72), A71 (= A73), G72 (= G74), T75 (≠ V77), N93 (= N95), T94 (= T96), N126 (= N130), C127 (= C131), G160 (= G164), G328 (= G324)

4r41A Complex crystal structure of 4-nitro-2-phosphono-benzoic acid with sp- aspartate-semialdehyde dehydrogenase and nicotinamide-dinucleotide (see paper)
46% identity, 95% coverage: 2:333/350 of query aligns to 2:337/357 of 4r41A

query
sites
4r41A

Y

Y

N

T

V

V

A

A

I

V

V

V

G
|
G

A

A

T
|
T

G
|
G

A
|
A

V

V

G

G

E

A

E

Q

L

M

F

I

R

K

V

M

L

L

E

E

Y

S

N

T

F

L

P

P

V

I

K

D

K

K

L

I

V

R

P

Y

L

L

A
|
A

S
|
S

A

A

R
|
R

S
|
S

V

A

G

G

K

K

E

S

V

L

E

K

F

F

K

K

G

D

E

Q

T

D

L

I

K

T

V

I

K

E

E

E

L
x
T

T

T

C

E

D

T

V

A

F

F

E

E

K

G

E

-

I

V

D

D

I

I

A

A

F

L

F

F

S
|
S

A
|
A

G
|
G

G
x
S

S

S

V

T

S

S

A

A

K

K

F

Y

A

A

P

P

C

Y

A

A

V

V

K

K

A

A

G

G

A

V

V

V

I

V

D

D

N
|
N

T
|
T

S
|
S

Y

Y

F

F

R
|
R

M

Q

D

N

K

P

D

D

V

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

E

H

D

-

I

-

A

A

L

L

W

D

R

A

K

H

K

N

G

G

I

I

A

A

N

C

P

P

N

N

C

C

S

S

T

T

I

I

Q

Q

M

M

V

M

I

V

S

A

L

L

A

E

P

P

L

V

H

R

K

Q

E

K

F

W

N

G

I

L

K

D

R

R

V

I

E

I

V

V

A

S

T

T

Y

Y

Q

Q

A

A

V

V

S

S

G

G

A

A

G
|
G

K
x
M

K
x
G

G

A

M

I

E

L

E

E

L

T

F

Q

E

R

Q

E

M

L

R

R

A

E

I

V

F

L

N

N

F

D

K

G

L

V

D

K

K

P

V

C

V

D

D

L

E

H

R

A

E

E

V

I

F

L

-

P

-

S

-

G

-

D

-

K

Q

H

Y

Y

Q

P

I

I

A

A

L

F

N

N

V

A

I

L

P

P

H

Q

I

I

D

D

R

V

F

F

M

T

D

D

N

N

G

D

F

Y

T

T

R

Y

E

E

M

M

K
|
K

M

M

V

T

N

K

E

E

T

T

N

K

K

K

I

I

M

M

H

E

S

D

N

D

-

S

I

I

E

A

V

V

A

S

P

A

T

T

C

C

V

V

R

R

V

I

P

P

V

V

L

L

R

S

S

A

H

H

S

S

E

E

A

S

I

V

T

Y

I

I

H

E

C

T

E

K

K

E

E

V

V

A

D

P

V

I

K

E

R

E

A

V

R

K

E

A

V

A

L

I

E

A

S

A

F

F

E

P

D

G

I

A

K

V

V

L

I

E

D

D

N

D

P

V

E

A

K

H

N

Q

E

I

Y

Y

P

P

M

Q

P

A

I

I

I

N

A

A

T

V

D

G

T

S

D

R

Y

D

V

T

Y

F

V

V

G

G

R

R

I

I

R

R

K

K

D

D

L

L

F

D

D

A

N

E

K

K

T

G

L

I

H

H

F

M

F

W

N

V

V

V

A

S

D

D

Q

N

L

L

R

L

V

K

G

G

A

A

T

W

N

N

A

S

V

V

R

Q

I

I

A

A

binding 4-nitro-2-phosphonobenzoic acid: S70 (= S72), G72 (= G74), S73 (≠ G75), N93 (= N95), T94 (= T96), S95 (= S97), R98 (= R100), K222 (= K219)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G8), T10 (= T10), G11 (= G11), A12 (= A12), A35 (= A35), S36 (= S36), R38 (= R38), S39 (= S39), T56 (≠ L56), A71 (= A73), G160 (= G164), M161 (≠ K165), G162 (≠ K166)

4r3nA Crystal structure of the ternary complex of sp-asadh with NADP and 1, 2,3-benzenetricarboxylic acid (see paper)
46% identity, 95% coverage: 2:333/350 of query aligns to 2:337/357 of 4r3nA

query
sites
4r3nA

Y

Y

N

T

V

V

A

A

I

V

V

V

G
|
G

A

A

T
|
T

G
|
G

A
|
A

V
|
V

G

G

E

A

E

Q

L

M

F

I

R

K

V

M

L

L

E

E

Y

S

N

T

F

L

P

P

V

I

K

D

K

K

L

I

V

R

P

Y

L

L

A
|
A

S
|
S

A

A

R

R

S
|
S

V

A

G

G

K

K

E

S

V

L

E

K

F

F

K

K

G

D

E

Q

T

D

L

I

K

T

V

I

K

E

E

E

L
x
T

T

T

C

E

D

T

V

A

F

F

E

E

K

G

E

-

I

V

D

D

I

I

A

A

F

L

F

F

S
|
S

A
|
A

G
|
G

G
x
S

S

S

V

T

S

S

A

A

K

K

F

Y

A

A

P

P

C

Y

A

A

V

V

K

K

A

A

G

G

A

V

V

V

I

V

D

D

N
|
N

T
|
T

S
|
S

Y

Y

F

F

R
|
R

M

Q

D

N

K

P

D

D

V

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

E

H

D

-

I

-

A

A

L

L

W

D

R

A

K

H

K

N

G

G

I

I

A

A

N

C

P

P

N
|
N

C
|
C

S

S

T

T

I

I

Q

Q

M

M

V

M

I

V

S

A

L

L

A

E

P

P

L

V

H

R

K

Q

E

K

F

W

N

G

I

L

K

D

R

R

V

I

E

I

V

V

A

S

T

T

Y

Y

Q
|
Q

A

A

V

V

S

S

G

G

A

A

G
|
G

K
x
M

K

G

G

A

M

I

E

L

E

E

L

T

F

Q

E

R

Q

E

M

L

R

R

A

E

I

V

F

L

N

N

F

D

K

G

L

V

D

K

K

P

V

C

V

D

D

L

E

H

R

A

E

E

V

I

F

L

-

P

-

S

-

G

-

D

-

K

Q

H

Y

Y

Q

P

I

I

A

A

L

F

N

N

V

A

I

L

P

P

H

Q

I

I

D

D

R

V

F

F

M

T

D

D

N

N

G

D

F

Y

T

T

R

Y

E

E

M

M

K
|
K

M

M

V

T

N

K

E

E

T

T

N

K

K

K

I

I

M

M

H

E

S

D

N

D

-

S

I

I

E

A

V

V

A

S

P

A

T

T

C

C

V

V

R
|
R

V

I

P

P

V

V

L

L

R

S

S

A

H
|
H

S

S

E

E

A

S

I

V

T

Y

I

I

H

E

C

T

E

K

K

E

E

V

V

A

D

P

V

I

K

E

R

E

A

V

R

K

E

A

V

A

L

I

E

A

S

A

F

F

E

P

D

G

I

A

K

V

V

L

I

E

D

D

N

D

P

V

E

A

K

H

N

Q

E

I

Y

Y

P

P

M

Q

P

A

I

I

I

N

A

A

T

V

D

G

T

S

D

R

Y

D

V

T

Y

F

V

V

G

G

R

R

I

I

R

R

K

K

D

D

L

L

F

D

D

A

N

E

K

K

T

G

L

I

H

H

F

M

F

W

N

V

V

V

A

S

D

D

Q

N

L

L

R

L

V

K

G
|
G

A

A

T

W

N

N

A

S

V

V

R

Q

I

I

A

A

active site: C127 (= C131), Q154 (= Q158), R244 (= R240), H251 (= H247)
binding benzene-1,2,3-tricarboxylic acid: S73 (≠ G75), T94 (= T96), S95 (= S97), R98 (= R100), N126 (= N130), K222 (= K219)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G8), T10 (= T10), G11 (= G11), A12 (= A12), V13 (= V13), A35 (= A35), S36 (= S36), S39 (= S39), T56 (≠ L56), S70 (= S72), A71 (= A73), G72 (= G74), N93 (= N95), T94 (= T96), N126 (= N130), C127 (= C131), G160 (= G164), M161 (≠ K165), G328 (= G324)

3q1lA Crystals structure of aspartate beta-semialdehyde dehydrogenase from streptococcus pneumoniae with cysteamine bound covalently to cys 128 (see paper)
46% identity, 95% coverage: 2:333/350 of query aligns to 2:337/357 of 3q1lA

query
sites
3q1lA

Y

Y

N

T

V

V

A

A

I

V

V

V

G

G

A

A

T

T

G

G

A

A

V

V

G

G

E

A

E

Q

L

M

F

I

R

K

V

M

L

L

E

E

Y

S

N

T

F

L

P

P

V

I

K

D

K

K

L

I

V

R

P

Y

L

L

A

A

S

S

A

A

R

R

S

S

V

A

G

G

K

K

E

S

V

L

E

K

F

F

K

K

G

D

E

Q

T

D

L

I

K

T

V

I

K

E

E

E

L

T

T

T

C

E

D

T

V

A

F

F

E

E

K

G

E

-

I

V

D

D

I

I

A

A

F

L

F

F

S

S

A

A

G

G

G

S

S

S

V

T

S

S

A

A

K

K

F

Y

A

A

P

P

C

Y

A

A

V

V

K

K

A

A

G

G

A

V

V

V

I

V

D

D

N

N

T

T

S

S

Y

Y

F

F

R

R

M

Q

D

N

K

P

D

D

V

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

E

H

D

-

I

-

A

A

L

L

W

D

R

A

K

H

K

N

G

G

I

I

A

A

N

C

P

P

N
|
N

C
|
C

S

S

T

T

I

I

Q

Q

M

M

V

M

I

V

S

A

L

L

A

E

P

P

L

V

H

R

K

Q

E

K

F

W

N

G

I

L

K

D

R

R

V

I

E

I

V

V

A

S

T

T

Y

Y

Q
|
Q

A

A

V

V

S

S

G
|
G

A

A

G

G

K

M

K

G

G

A

M

I

E

L

E

E

L

T

F

Q

E

R

Q

E

M

L

R

R

A

E

I

V

F

L

N

N

F

D

K

G

L

V

D

K

K

P

V

C

V

D

D

L

E

H

R

A

E

E

V

I

F

L

-

P

-

S

-

G

-

D

-

K

Q

H

Y

Y

Q

P

I

I

A

A

L

F

N

N

V

A

I

L

P

P

H

Q

I

I

D

D

R

V

F

F

M

T

D

D

N

N

G

D

F

Y

T

T

R

Y

E
|
E

E

E

M

M

K

K

M

M

V

T

N

K

E

E

T

T

N

K

K

K

I

I

M

M

H

E

S

D

N

D

-

S

I

I

E

A

V

V

A

S

P

A

T

T

C

C

V

V

R
|
R

V

I

P

P

V

V

L

L

R

S

S

A

H
|
H

S

S

E

E

A

S

I

V

T

Y

I

I

H

E

C

T

E

K

K

E

E

V

V

A

D

P

V

I

K

E

R

E

A

V

R

K

E

A

V

A

L

I

E

A

S

A

F

F

E

P

D

G

I

A

K

V

V

L

I

E

D

D

N

D

P

V

E

A

K

H

N

Q

E

I

Y

Y

P

P

M

Q

P

A

I

I

I

N

A

A

T

V

D

G

T

S

D

R

Y

D

V

T

Y

F

V

V

G

G

R

R

I

I

R

R

K

K

D

D

L

L

F

D

D

A

N

E

K

K

T

G

L

I

H

H

F

M

F

W

N

V

V

V

A

S

D

D

Q

N

L

L

R

L

V

K

G

G

A

A

T

W

N

N

A

S

V

V

R

Q

I

I

A

A

active site: C127 (= C131), Q154 (= Q158), R244 (= R240), H251 (= H247)
binding 2-amino-ethanethiol: N126 (= N130), C127 (= C131), G158 (= G162), E219 (= E216)

3pwsA Crystal structure of aspartate beta-semialdehide dehydrogenase from streptococcus pneumoniae with 2',5'-adenosine diphosphate and d-2- aminoadipate (see paper)
46% identity, 95% coverage: 2:333/350 of query aligns to 2:337/357 of 3pwsA

query
sites
3pwsA

Y

Y

N

T

V

V

A

A

I

V

V

V

G
|
G

A

A

T
|
T

G
|
G

A
|
A

V

V

G

G

E

A

E

Q

L

M

F

I

R

K

V

M

L

L

E

E

Y

S

N

T

F

L

P

P

V

I

K

D

K

K

L

I

V

R

P

Y

L

L

A
|
A

S
|
S

A

A

R
|
R

S
|
S

V

A

G

G

K

K

E

S

V

L

E

K

F

F

K

K

G

D

E

Q

T

D

L

I

K

T

V

I

K

E

E

E

L
x
T

T

T

C

E

D

T

V

A

F

F

E

E

K

G

E

-

I

V

D

D

I

I

A

A

F

L

F

F

S

S

A
|
A

G

G

G

S

S

S

V
x
T

S

S

A

A

K

K

F

Y

A

A

P

P

C

Y

A

A

V

V

K

K

A

A

G

G

A

V

V

V

I

V

D

D

N

N

T

T

S

S

Y

Y

F

F

R
|
R

M

Q

D

N

K

P

D

D

V

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

E

H

D

-

I

-

A

A

L

L

W

D

R

A

K

H

K

N

G

G

I

I

A

A

N

C

P

P

N
|
N

C

C

S

S

T

T

I

I

Q

Q

M

M

V

M

I

V

S

A

L

L

A

E

P

P

L

V

H

R

K

Q

E

K

F

W

N

G

I

L

K

D

R

R

V

I

E

I

V

V

A

S

T

T

Y

Y

Q

Q

A

A

V

V

S

S

G
|
G

A

A

G
|
G

K
x
M

K

G

G

A

M

I

E

L

E

E

L

T

F

Q

E

R

Q

E

M

L

R

R

A

E

I

V

F

L

N

N

F

D

K

G

L

V

D

K

K

P

V

C

V

D

D

L

E

H

R

A

E

E

V

I

F

L

-

P

-

S

-

G

-

D

-

K

Q

H

Y

Y

Q

P

I

I

A

A

L

F

N

N

V

A

I

L

P

P

H

Q

I
|
I

D

D

R

V

F

F

M

T

D

D

N

N

G

D

F

Y

T

T

R

Y

E
|
E

E

E

M

M

K
|
K

M

M

V

T

N

K

E

E

T

T

N

K

K

K

I

I

M

M

H

E

S

D

N

D

-

S

I

I

E

A

V

V

A

S

P

A

T

T

C

C

V

V

R
|
R

V

I

P

P

V

V

L

L

R

S

S

A

H

H

S

S

E

E

A

S

I

V

T

Y

I

I

H

E

C

T

E

K

K

E

E

V

V

A

D

P

V

I

K

E

R

E

A

V

R

K

E

A

V

A

L

I

E

A

S

A

F

F

E

P

D

G

I

A

K

V

V

L

I

E

D

D

N

D

P

V

E

A

K

H

N

Q

E

I

Y

Y

P

P

M

Q

P

A

I

I

I

N

A

A

T

V

D

G

T

S

D

R

Y

D

V

T

Y

F

V

V

G

G

R

R

I

I

R

R

K

K

D

D

L

L

F

D

D

A

N

E

K

K

T

G

L

I

H

H

F

M

F

W

N

V

V

V

A

S

D

D

Q

N

L

L

R

L

V

K

G

G

A

A

T

W

N

N

A

S

V

V

R

Q

I

I

A

A

binding (2R)-2-aminohexanedioic acid: R98 (= R100), N126 (= N130), G158 (= G162), I208 (= I205), E219 (= E216), K222 (= K219), R244 (= R240)
binding adenosine-2'-5'-diphosphate: G8 (= G8), T10 (= T10), G11 (= G11), A12 (= A12), A35 (= A35), S36 (= S36), R38 (= R38), S39 (= S39), T56 (≠ L56), A71 (= A73), T75 (≠ V77), G160 (= G164), M161 (≠ K165)

3pwkA Crystal structure of aspartate beta-semialdehide dehydrogenase from streptococcus pneumoniae with 2',5'-adenosine diphosphate and d-2- aminoadipate (see paper)
46% identity, 95% coverage: 2:333/350 of query aligns to 2:337/357 of 3pwkA

query
sites
3pwkA

Y

Y

N

T

V

V

A

A

I

V

V

V

G
|
G

A

A

T
|
T

G
|
G

A
|
A

V

V

G

G

E

A

E

Q

L

M

F

I

R

K

V

M

L

L

E

E

Y

S

N

T

F

L

P

P

V

I

K

D

K

K

L

I

V

R

P

Y

L

L

A
|
A

S
|
S

A

A

R
|
R

S
|
S

V

A

G

G

K

K

E

S

V

L

E

K

F

F

K

K

G

D

E

Q

T

D

L

I

K

T

V

I

K

E

E

E

L
x
T

T

T

C

E

D

T

V

A

F

F

E

E

K

G

E

-

I

V

D

D

I

I

A

A

F

L

F

F

S

S

A
|
A

G

G

G

S

S

S

V
x
T

S

S

A

A

K

K

F

Y

A

A

P

P

C

Y

A

A

V

V

K

K

A

A

G

G

A

V

V

V

I

V

D

D

N

N

T

T

S

S

Y

Y

F

F

R
|
R

M

Q

D

N

K

P

D

D

V

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

E

H

D

-

I

-

A

A

L

L

W

D

R

A

K

H

K

N

G

G

I

I

A

A

N

C

P

P

N
|
N

C

C

S

S

T

T

I

I

Q

Q

M

M

V

M

I

V

S

A

L

L

A

E

P

P

L

V

H

R

K

Q

E

K

F

W

N

G

I

L

K

D

R

R

V

I

E

I

V

V

A

S

T

T

Y

Y

Q

Q

A

A

V

V

S

S

G
|
G

A
|
A

G
|
G

K

M

K

G

G

A

M

I

E

L

E

E

L

T

F

Q

E

R

Q

E

M

L

R

R

A

E

I

V

F

L

N

N

F

D

K

G

L

V

D

K

K

P

V

C

V

D

D

L

E

H

R

A

E

E

V

I

F

L

-

P

-

S

-

G

-

D

-

K

Q

H

Y

Y

Q

P

I

I

A

A

L

F

N

N

V

A

I

L

P

P

H

Q

I

I

D

D

R

V

F

F

M

T

D

D

N

N

G

D

F

Y

T

T

R

Y

E
|
E

E

E

M

M

K
|
K

M

M

V

T

N

K

E

E

T

T

N

K

K

K

I

I

M

M

H

E

S

D

N

D

-

S

I

I

E

A

V

V

A

S

P

A

T

T

C

C

V

V

R
|
R

V

I

P

P

V

V

L

L

R

S

S

A

H

H

S

S

E

E

A

S

I

V

T

Y

I

I

H

E

C

T

E

K

K

E

E

V

V

A

D

P

V

I

K

E

R

E

A

V

R

K

E

A

V

A

L

I

E

A

S

A

F

F

E

P

D

G

I

A

K

V

V

L

I

E

D

D

N

D

P

V

E

A

K

H

N

Q

E

I

Y

Y

P

P

M

Q

P

A

I

I

I

N

A

A

T

V

D

G

T

S

D

R

Y

D

V

T

Y

F

V

V

G

G

R

R

I

I

R

R

K

K

D

D

L

L

F

D

D

A

N

E

K

K

T

G

L

I

H

H

F

M

F

W

N

V

V

V

A

S

D

D

Q

N

L

L

R

L

V

K

G

G

A

A

T

W

N

N

A

S

V

V

R

Q

I

I

A

A

binding 5'-o-monophosphoryladenylyl(2'->5')adenylyl(2'->5')adenosine: G8 (= G8), T10 (= T10), G11 (= G11), A12 (= A12), A35 (= A35), S36 (= S36), R38 (= R38), S39 (= S39), T56 (≠ L56), A71 (= A73), T75 (≠ V77), G160 (= G164)
binding trans-cyclohexane-1,4-dicarboxylic acid: R98 (= R100), N126 (= N130), G158 (= G162), A159 (= A163), E219 (= E216), K222 (= K219), R244 (= R240)

2gz3A Structure of aspartate semialdehyde dehydrogenase (asadh) from streptococcus pneumoniae complexed with NADP and aspartate- semialdehyde (see paper)
46% identity, 95% coverage: 2:333/350 of query aligns to 2:337/357 of 2gz3A

query
sites
2gz3A

Y

Y

N

T

V

V

A

A

I

V

V

V

G
|
G

A

A

T
|
T

G
|
G

A
|
A

V
|
V

G

G

E

A

E

Q

L

M

F

I

R

K

V

M

L

L

E

E

Y

S

N

T

F

L

P

P

V

I

K

D

K

K

L

I

V

R

P

Y

L

L

A
|
A

S
|
S

A

A

R
|
R

S
|
S

V

A

G

G

K

K

E

S

V

L

E

K

F

F

K

K

G

D

E

Q

T

D

L

I

K

T

V

I

K

E

E

E

L
x
T

T

T

C

E

D

T

V

A

F

F

E

E

K

G

E

-

I

V

D

D

I

I

A

A

F

L

F

F

S
|
S

A
|
A

G
|
G

G

S

S

S

V
x
T

S

S

A

A

K

K

F

Y

A

A

P

P

C

Y

A

A

V

V

K

K

A

A

G

G

A

V

V

V

I

V

D

D

N
|
N

T

T

S

S

Y

Y

F

F

R

R

M

Q

D

N

K

P

D

D

V

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

E

H

D

-

I

-

A

A

L

L

W

D

R

A

K

H

K

N

G

G

I

I

A

A

N

C

P

P

N

N

C
|
C

S

S

T

T

I

I

Q

Q

M

M

V

M

I

V

S

A

L

L

A

E

P

P

L

V

H

R

K

Q

E

K

F

W

N

G

I

L

K

D

R

R

V

I

E

I

V

V

A

S

T

T

Y

Y

Q
|
Q

A

A

V

V

S

S

G
|
G

A

A

G
|
G

K
x
M

K

G

G

A

M

I

E

L

E

E

L

T

F

Q

E

R

Q

E

M

L

R

R

A

E

I

V

F

L

N

N

F

D

K

G

L

V

D

K

K

P

V

C

V

D

D

L

E

H

R

A

E

E

V

I

F

L

-

P

-

S

-

G

-

D

-

K

Q

H

Y

Y

Q

P

I

I

A

A

L

F

N

N

V

A

I

L

P

P

H

Q

I

I

D

D

R

V

F

F

M

T

D

D

N

N

G

D

F

Y

T

T

R

Y

E
|
E

E

E

M

M

K

K

M

M

V

T

N

K

E

E

T

T

N

K

K

K

I

I

M

M

H

E

S

D

N

D

-

S

I

I

E

A

V

V

A

S

P

A

T

T

C

C

V

V

R
|
R

V

I

P

P

V

V

L

L

R

S

S

A

H
|
H

S

S

E

E

A

S

I

V

T

Y

I

I

H

E

C

T

E

K

K

E

E

V

V

A

D

P

V

I

K

E

R

E

A

V

R

K

E

A

V

A

L

I

E

A

S

A

F

F

E

P

D

G

I

A

K

V

V

L

I

E

D

D

N

D

P

V

E

A

K

H

N

Q

E

I

Y

Y

P

P

M

Q

P

A

I

I

I

N

A

A

T

V

D

G

T

S

D

R

Y

D

V

T

Y

F

V

V

G

G

R

R

I

I

R

R

K

K

D

D

L

L

F

D

D

A

N

E

K

K

T

G

L

I

H

H

F

M

F

W

N

V

V

V

A

S

D

D

Q
x
N

L

L

R

L

V

K

G

G

A
|
A

A

A

T

W

N

N

A

S

V

V

R

Q

I

I

A

A

active site: C127 (= C131), Q154 (= Q158), R244 (= R240), H251 (= H247)
binding (2r)-2-amino-4-oxobutanoic acid: C127 (= C131), Q154 (= Q158), G158 (= G162), E219 (= E216), R244 (= R240), H251 (= H247)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G8), T10 (= T10), G11 (= G11), A12 (= A12), V13 (= V13), A35 (= A35), S36 (= S36), R38 (= R38), S39 (= S39), T56 (≠ L56), S70 (= S72), A71 (= A73), G72 (= G74), T75 (≠ V77), N93 (= N95), G158 (= G162), G160 (= G164), M161 (≠ K165), N324 (≠ Q320), A329 (= A325)

2gz2A Structure of aspartate semialdehyde dehydrogenase (asadh) from streptococcus pneumoniae complexed with 2',5'-adp (see paper)
46% identity, 95% coverage: 2:333/350 of query aligns to 2:337/357 of 2gz2A

query
sites
2gz2A

Y

Y

N

T

V

V

A

A

I

V

V

V

G
|
G

A

A

T
|
T

G
|
G

A

A

V

V

G

G

E

A

E

Q

L

M

F

I

R

K

V

M

L

L

E

E

Y

S

N

T

F

L

P

P

V

I

K

D

K

K

L

I

V

R

P

Y

L

L

A
|
A

S
|
S

A

A

R
|
R

S
|
S

V

A

G

G

K

K

E

S

V

L

E

K

F

F

K

K

G

D

E

Q

T

D

L

I

K

T

V

I

K

E

E

E

L
x
T

T

T

C

E

D

T

V

A

F

F

E

E

K

G

E

-

I

V

D

D

I

I

A

A

F

L

F

F

S

S

A
|
A

G

G

G

S

S

S

V
x
T

S

S

A

A

K

K

F

Y

A

A

P

P

C

Y

A

A

V

V

K

K

A

A

G

G

A

V

V

V

I

V

D

D

N

N

T

T

S

S

Y

Y

F

F

R

R

M

Q

D

N

K

P

D

D

V

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

E

H

D

-

I

-

A

A

L

L

W

D

R

A

K

H

K

N

G

G

I

I

A

A

N

C

P

P

N

N

C
|
C

S

S

T

T

I

I

Q

Q

M

M

V

M

I

V

S

A

L

L

A

E

P

P

L

V

H

R

K

Q

E

K

F

W

N

G

I

L

K

D

R

R

V

I

E

I

V

V

A

S

T

T

Y

Y

Q
|
Q

A

A

V

V

S

S

G

G

A

A

G

G

K

M

K

G

G

A

M

I

E

L

E

E

L

T

F

Q

E

R

Q

E

M

L

R

R

A

E

I

V

F

L

N

N

F

D

K

G

L

V

D

K

K

P

V

C

V

D

D

L

E

H

R

A

E

E

V

I

F

L

-

P

-

S

-

G

-

D

-

K

Q

H

Y

Y

Q

P

I

I

A

A

L

F

N

N

V

A

I

L

P

P

H

Q

I

I

D

D

R

V

F

F

M

T

D

D

N

N

G

D

F

Y

T

T

R

Y

E

E

M

M

K

K

M

M

V

T

N

K

E

E

T

T

N

K

K

K

I

I

M

M

H

E

S

D

N

D

-

S

I

I

E

A

V

V

A

S

P

A

T

T

C

C

V

V

R
|
R

V

I

P

P

V

V

L

L

R

S

S

A

H
|
H

S

S

E

E

A

S

I

V

T

Y

I

I

H

E

C

T

E

K

K

E

E

V

V

A

D

P

V

I

K

E

R

E

A

V

R

K

E

A

V

A

L

I

E

A

S

A

F

F

E

P

D

G

I

A

K

V

V

L

I

E

D

D

N

D

P

V

E

A

K

H

N

Q

E

I

Y

Y

P

P

M

Q

P

A

I

I

I

N

A

A

T

V

D

G

T

S

D

R

Y

D

V

T

Y

F

V

V

G

G

R

R

I

I

R

R

K

K

D

D

L

L

F

D

D

A

N

E

K

K

T

G

L

I

H

H

F

M

F

W

N

V

V

V

A

S

D

D

Q

N

L

L

R

L

V

K

G

G

A

A

T

W

N

N

A

S

V

V

R

Q

I

I

A

A

active site: C127 (= C131), Q154 (= Q158), R244 (= R240), H251 (= H247)
binding adenosine-2'-5'-diphosphate: G8 (= G8), T10 (= T10), G11 (= G11), A35 (= A35), S36 (= S36), R38 (= R38), S39 (= S39), T56 (≠ L56), A71 (= A73), T75 (≠ V77)

2gz1A Structure of aspartate semialdehyde dehydrogenase (asadh) from streptococcus pneumoniae complexed with NADP (see paper)
46% identity, 95% coverage: 2:333/350 of query aligns to 2:337/357 of 2gz1A

query
sites
2gz1A

Y

Y

N

T

V

V

A

A

I

V

V

V

G
|
G

A

A

T
|
T

G
|
G

A
|
A

V
|
V

G

G

E

A

E

Q

L

M

F

I

R

K

V

M

L

L

E

E

Y

S

N

T

F

L

P

P

V

I

K

D

K

K

L

I

V

R

P

Y

L

L

A
|
A

S
|
S

A

A

R
|
R

S
|
S

V

A

G

G

K

K

E

S

V

L

E

K

F

F

K

K

G

D

E

Q

T

D

L

I

K

T

V

I

K

E

E

E

L
x
T

T

T

C

E

D

T

V

A

F

F

E

E

K

G

E

-

I

V

D

D

I

I

A

A

F

L

F

F

S
|
S

A
|
A

G
|
G

G

S

S

S

V
x
T

S

S

A

A

K

K

F

Y

A

A

P

P

C

Y

A

A

V

V

K

K

A

A

G

G

A

V

V

V

I

V

D

D

N
|
N

T

T

S

S

Y

Y

F

F

R

R

M

Q

D

N

K

P

D

D

V

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

E

H

D

-

I

-

A

A

L

L

W

D

R

A

K

H

K

N

G

G

I

I

A

A

N

C

P

P

N

N

C
|
C

S

S

T

T

I

I

Q

Q

M

M

V

M

I

V

S

A

L

L

A

E

P

P

L

V

H

R

K

Q

E

K

F

W

N

G

I

L

K

D

R

R

V

I

E

I

V

V

A

S

T

T

Y

Y

Q
|
Q

A

A

V

V

S
|
S

G
|
G

A

A

G
|
G

K
x
M

K

G

G

A

M

I

E

L

E

E

L

T

F

Q

E

R

Q

E

M

L

R

R

A

E

I

V

F

L

N

N

F

D

K

G

L

V

D

K

K

P

V

C

V

D

D

L

E

H

R

A

E

E

V

I

F

L

-

P

-

S

-

G

-

D

-

K

Q

H

Y

Y

Q

P

I

I

A

A

L

F

N

N

V

A

I

L

P

P

H

Q

I

I

D

D

R

V

F

F

M

T

D

D

N

N

G

D

F

Y

T

T

R

Y

E

E

M

M

K

K

M

M

V

T

N

K

E

E

T

T

N

K

K

K

I

I

M

M

H

E

S

D

N

D

-

S

I

I

E

A

V

V

A

S

P

A

T

T

C

C

V

V

R
|
R

V

I

P

P

V

V

L

L

R

S

S

A

H
|
H

S

S

E

E

A

S

I

V

T

Y

I

I

H

E

C

T

E

K

K

E

E

V

V

A

D

P

V

I

K

E

R

E

A

V

R

K

E

A

V

A

L

I

E

A

S

A

F

F

E

P

D

G

I

A

K

V

V

L

I

E

D

D

N

D

P

V

E

A

K

H

N

Q

E

I

Y

Y

P

P

M

Q

P

A

I

I

I

N

A

A

T

V

D

G

T

S

D

R

Y

D

V

T

Y

F

V

V

G

G

R

R

I

I

R

R

K

K

D

D

L

L

F

D

D

A

N

E

K

K

T

G

L

I

H

H

F

M

F

W

N

V

V

V

A

S

D

D

Q
x
N

L
|
L

R

L

V

K

G

G

A

A

T

W

N

N

A

S

V

V

R

Q

I

I

A

A

active site: C127 (= C131), Q154 (= Q158), R244 (= R240), H251 (= H247)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G8), T10 (= T10), G11 (= G11), A12 (= A12), V13 (= V13), A35 (= A35), S36 (= S36), R38 (= R38), S39 (= S39), T56 (≠ L56), S70 (= S72), A71 (= A73), G72 (= G74), T75 (≠ V77), N93 (= N95), S157 (= S161), G158 (= G162), G160 (= G164), M161 (≠ K165), N324 (≠ Q320), L325 (= L321)

3q11A Crystals structure of aspartate beta-semialdehyde dehydrogenase from streptococcus pneumoniae with NADP and aspartyl beta- difluorophosphonate (see paper)
46% identity, 95% coverage: 2:333/350 of query aligns to 2:337/358 of 3q11A

query
sites
3q11A

Y

Y

N

T

V

V

A

A

I

V

V

V

G
|
G

A

A

T
|
T

G
|
G

A

A

V

V

G

G

E

A

E

Q

L

M

F

I

R

K

V

M

L

L

E

E

Y

S

N

T

F

L

P

P

V

I

K

D

K

K

L

I

V

R

P

Y

L

L

A
|
A

S
|
S

A

A

R
|
R

S
|
S

V

A

G

G

K

K

E

S

V

L

E

K

F

F

K

K

G

D

E

Q

T

D

L

I

K

T

V

I

K

E

E

E

L
x
T

T

T

C

E

D

T

V

A

F

F

E

E

K

G

E

-

I

V

D

D

I

I

A

A

F

L

F

F

S

S

A
|
A

G

G

G

S

S

S

V
x
T

S

S

A

A

K

K

F

Y

A

A

P

P

C

Y

A

A

V

V

K

K

A

A

G

G

A

V

V

V

I

V

D

D

N

N

T

T

S

S

Y

Y

F

F

R
|
R

M

Q

D

N

K

P

D

D

V

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

E

H

D

-

I

-

A

A

L

L

W

D

R

A

K

H

K

N

G

G

I

I

A

A

N

C

P

P

N
|
N

C
|
C

S

S

T

T

I

I

Q

Q

M

M

V

M

I

V

S

A

L

L

A

E

P

P

L

V

H

R

K

Q

E

K

F

W

N

G

I

L

K

D

R

R

V

I

E

I

V

V

A

S

T

T

Y

Y

Q
|
Q

A

A

V

V

S

S

G
|
G

A

A

G
|
G

K
x
M

K
x
G

G

A

M

I

E

L

E

E

L

T

F

Q

E

R

Q

E

M

L

R

R

A

E

I

V

F

L

N

N

F

D

K

G

L

V

D

K

K

P

V

C

V

D

D

L

E

H

R

A

E

E

V

I

F

L

-

P

-

S

-

G

-

D

-

K

Q

H

Y

Y

Q

P

I

I

A

A

L

F

N

N

V

A

I

L

P

P

H

Q

I

I

D

D

R

V

F

F

M

T

D

D

N

N

G

D

F

Y

T

T

R

Y

E
|
E

E

E

M

M

K
|
K

M

M

V

T

N

K

E

E

T

T

N

K

K

K

I

I

M

M

H

E

S

D

N

D

-

S

I

I

E

A

V

V

A

S

P

A

T

T

C

C

V

V

R
|
R

V

I

P

P

V

V

L

L

R

S

S

A

H

H

S

S

E

E

A

S

I

V

T

Y

I

I

H

E

C

T

E

K

K

E

E

V

V

A

D

P

V

I

K

E

R

E

A

V

R

K

E

A

V

A

L

I

E

A

S

A

F

F

E

P

D

G

I

A

K

V

V

L

I

E

D

D

N

D

P

V

E

A

K

H

N

Q

E

I

Y

Y

P

P

M

Q

P

A

I

I

I

N

A

A

T

V

D

G

T

S

D

R

Y

D

V

T

Y

F

V

V

G

G

R

R

I

I

R

R

K

K

D

D

L

L

F

D

D

A

N

E

K

K

T

G

L

I

H

H

F

M

F

W

N

V

V

V

A

S

D

D

Q

N

L

L

R

L

V

K

G

G

A

A

T

W

N

N

A

S

V

V

R

Q

I

I

A

A

binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G8), T10 (= T10), G11 (= G11), A35 (= A35), S36 (= S36), R38 (= R38), S39 (= S39), T56 (≠ L56), A71 (= A73), T75 (≠ V77), G160 (= G164), M161 (≠ K165), G162 (≠ K166)
binding 5,5-difluoro-4-oxo-5-phosphono-D-norvaline: R98 (= R100), N126 (= N130), C127 (= C131), Q154 (= Q158), G158 (= G162), E219 (= E216), K222 (= K219), R244 (= R240)

4r5hA Crystal structure of sp-aspartate-semialdehyde-dehydrogenase with nicotinamide-adenine-dinucleotide-phosphate and 3-carboxy-propenyl- phthalic acid (see paper)
46% identity, 95% coverage: 2:333/350 of query aligns to 2:337/359 of 4r5hA

query
sites
4r5hA

Y

Y

N

T

V

V

A

A

I

V

V

V

G
|
G

A

A

T
|
T

G
|
G

A
|
A

V
|
V

G

G

E

A

E

Q

L

M

F

I

R

K

V

M

L

L

E

E

Y

S

N

T

F

L

P

P

V

I

K

D

K

K

L

I

V

R

P

Y

L

L

A
|
A

S
|
S

A

A

R
|
R

S
|
S

V

A

G

G

K

K

E

S

V

L

E

K

F

F

K

K

G

D

E

Q

T

D

L

I

K

T

V

I

K

E

E

E

L
x
T

T

T

C

E

D

T

V

A

F

F

E

E

K

G

E

-

I

V

D

D

I

I

A

A

F

L

F

F

S
|
S

A
|
A

G
|
G

G
x
S

S

S

V
x
T

S

S

A

A

K

K

F

Y

A

A

P

P

C

Y

A

A

V

V

K

K

A

A

G

G

A

V

V

V

I

V

D

D

N
|
N

T
|
T

S
|
S

Y

Y

F

F

R
|
R

M

Q

D

N

K

P

D

D

V

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

E

H

D

-

I

-

A

A

L

L

W

D

R

A

K

H

K

N

G

G

I

I

A

A

N

C

P
|
P

N
|
N

C
|
C

S

S

T

T

I

I

Q

Q

M

M

V

M

I

V

S

A

L

L

A

E

P

P

L

V

H

R

K

Q

E

K

F

W

N

G

I

L

K

D

R

R

V

I

E

I

V

V

A

S

T

T

Y

Y

Q
|
Q

A

A

V

V

S

S

G
|
G

A

A

G
|
G

K
x
M

K

G

G

A

M

I

E

L

E

E

L

T

F

Q

E

R

Q

E

M

L

R

R

A

E

I

V

F

L

N

N

F

D

K

G

L

V

D

K

K

P

V

C

V

D

D

L

E

H

R

A

E

E

V

I

F

L

-

P

-

S

-

G

-

D

-

K

Q

H

Y

Y

Q

P

I

I

A

A

L

F

N

N

V

A

I

L

P

P

H

Q

I

I

D

D

R

V

F

F

M

T

D

D

N

N

G

D

F

Y

T

T

R

Y

E

E

M

M

K
|
K

M

M

V

T

N

K

E

E

T

T

N

K

K

K

I

I

M

M

H

E

S

D

N

D

-

S

I

I

E

A

V

V

A

S

P

A

T

T

C

C

V

V

R
|
R

V

I

P

P

V

V

L

L

R

S

S

A

H
|
H

S

S

E

E

A

S

I

V

T

Y

I

I

H

E

C

T

E

K

K

E

E

V

V

A

D

P

V

I

K

E

R

E

A

V

R

K

E

A

V

A

L

I

E

A

S

A

F

F

E

P

D

G

I

A

K

V

V

L

I

E

D

D

N

D

P

V

E

A

K

H

N

Q

E

I

Y

Y

P

P

M

Q

P

A

I

I

I

N

A

A

T

V

D

G

T

S

D

R

Y

D

V

T

Y

F

V

V

G

G

R

R

I

I

R

R

K

K

D

D

L

L

F

D

D

A

N

E

K

K

T

G

L

I

H

H

F

M

F

W

N

V

V

V

A

S

D

D

Q

N

L

L

R

L

V

K

G
|
G

A

A

T

W

N

N

A

S

V

V

R

Q

I

I

A

A

binding 3-[(1E)-3-carboxyprop-1-en-1-yl]benzene-1,2-dicarboxylic acid: S73 (≠ G75), T94 (= T96), S95 (= S97), R98 (= R100), N126 (= N130), C127 (= C131), Q154 (= Q158), G158 (= G162), K222 (= K219), R244 (= R240), H251 (= H247)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G8), T10 (= T10), G11 (= G11), A12 (= A12), V13 (= V13), A35 (= A35), S36 (= S36), R38 (= R38), S39 (= S39), T56 (≠ L56), S70 (= S72), A71 (= A73), G72 (= G74), T75 (≠ V77), N93 (= N95), T94 (= T96), P125 (= P129), N126 (= N130), C127 (= C131), G160 (= G164), M161 (≠ K165), G328 (= G324)

4r4jA Crystal structure of complex sp_asadh with 3-carboxypropyl-phthalic acid and nicotinamide adenine dinucleotide phosphate (see paper)
46% identity, 95% coverage: 2:333/350 of query aligns to 2:337/359 of 4r4jA

query
sites
4r4jA

Y

Y

N

T

V

V

A

A

I

V

V

V

G
|
G

A

A

T
|
T

G
|
G

A
|
A

V
|
V

G

G

E

A

E

Q

L

M

F

I

R

K

V

M

L

L

E

E

Y

S

N

T

F

L

P

P

V

I

K

D

K

K

L

I

V

R

P

Y

L

L

A
|
A

S
|
S

A

A

R
|
R

S
|
S

V

A

G

G

K

K

E

S

V

L

E

K

F

F

K

K

G

D

E

Q

T

D

L

I

K

T

V

I

K

E

E

E

L
x
T

T

T

C

E

D

T

V

A

F

F

E

E

K

G

E

-

I

V

D

D

I

I

A

A

F

L

F

F

S
|
S

A
|
A

G
|
G

G

S

S

S

V
x
T

S

S

A

A

K

K

F

Y

A

A

P

P

C

Y

A

A

V

V

K

K

A

A

G

G

A

V

V

V

I

V

D

D

N
|
N

T
|
T

S
|
S

Y

Y

F

F

R
|
R

M

Q

D

N

K

P

D

D

V

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

E

H

D

-

I

-

A

A

L

L

W

D

R

A

K

H

K

N

G

G

I

I

A

A

N

C

P

P

N
|
N

C
|
C

S

S

T

T

I

I

Q

Q

M

M

V

M

I

V

S

A

L

L

A

E

P

P

L

V

H

R

K

Q

E

K

F

W

N

G

I

L

K

D

R

R

V

I

E

I

V

V

A

S

T

T

Y

Y

Q
|
Q

A

A

V

V

S

S

G
|
G

A

A

G
|
G

K
x
M

K

G

G

A

M

I

E

L

E

E

L

T

F

Q

E

R

Q

E

M

L

R

R

A

E

I

V

F

L

N

N

F

D

K

G

L

V

D

K

K

P

V

C

V

D

D

L

E

H

R

A

E

E

V

I

F

L

-

P

-

S

-

G

-

D

-

K

Q

H

Y

Y

Q

P

I

I

A

A

L

F

N

N

V

A

I

L

P

P

H

Q

I

I

D

D

R

V

F

F

M

T

D

D

N

N

G

D

F

Y

T

T

R

Y

E
|
E

E

E

M

M

K
|
K

M

M

V

T

N

K

E

E

T

T

N

K

K

K

I

I

M

M

H

E

S

D

N

D

-

S

I

I

E

A

V

V

A

S

P

A

T

T

C

C

V

V

R
|
R

V

I

P

P

V

V

L

L

R

S

S

A

H
|
H

S

S

E

E

A

S

I

V

T

Y

I

I

H

E

C

T

E

K

K

E

E

V

V

A

D

P

V

I

K

E

R

E

A

V

R

K

E

A

V

A

L

I

E

A

S

A

F

F

E

P

D

G

I

A

K

V

V

L

I

E

D

D

N

D

P

V

E

A

K

H

N

Q

E

I

Y

Y

P

P

M

Q

P

A

I

I

I

N

A

A

T

V

D

G

T

S

D

R

Y

D

V

T

Y

F

V

V

G

G

R

R

I

I

R

R

K

K

D

D

L

L

F

D

D

A

N

E

K

K

T

G

L

I

H

H

F

M

F

W

N

V

V

V

A

S

D

D

Q

N

L

L

R

L

V

K

G
|
G

A

A

T

W

N

N

A

S

V

V

R

Q

I

I

A

A

binding 3-(3-carboxypropyl)benzene-1,2-dicarboxylic acid: T94 (= T96), S95 (= S97), R98 (= R100), N126 (= N130), C127 (= C131), Q154 (= Q158), G158 (= G162), E219 (= E216), K222 (= K219), R244 (= R240), H251 (= H247)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G8), T10 (= T10), G11 (= G11), A12 (= A12), V13 (= V13), A35 (= A35), S36 (= S36), R38 (= R38), S39 (= S39), T56 (≠ L56), S70 (= S72), A71 (= A73), G72 (= G74), T75 (≠ V77), N93 (= N95), T94 (= T96), N126 (= N130), C127 (= C131), G160 (= G164), M161 (≠ K165), G328 (= G324)

3pyxB Crystals structure of aspartate beta-semialdehyde dehydrogenase complex with NADP and 2-aminoterephthalate (see paper)
46% identity, 95% coverage: 2:333/350 of query aligns to 2:337/359 of 3pyxB

query
sites
3pyxB

Y

Y

N

T

V

V

A

A

I

V

V

V

G
|
G

A

A

T
|
T

G
|
G

A
|
A

V
|
V

G

G

E

A

E

Q

L

M

F

I

R

K

V

M

L

L

E

E

Y

S

N

T

F

L

P

P

V

I

K

D

K

K

L

I

V

R

P

Y

L

L

A
|
A

S
|
S

A

A

R
|
R

S
|
S

V

A

G

G

K

K

E

S

V

L

E

K

F

F

K

K

G

D

E

Q

T

D

L

I

K

T

V

I

K

E

E

E

L
x
T

T

T

C

E

D

T

V

A

F

F

E

E

K

G

E

-

I

V

D

D

I

I

A

A

F

L

F

F

S
|
S

A
|
A

G
|
G

G

S

S

S

V
x
T

S

S

A

A

K

K

F

Y

A

A

P

P

C

Y

A

A

V

V

K

K

A

A

G

G

A

V

V

V

I

V

D

D

N

N

T

T

S

S

Y

Y

F

F

R
|
R

M

Q

D

N

K

P

D

D

V

V

P

P

L

L

V

V

P

P

E

E

V

V

N

N

P

A

E

H

D

-

I

-

A

A

L

L

W

D

R

A

K

H

K

N

G

G

I

I

A

A

N

C

P

P

N

N

C
|
C

S

S

T

T

I

I

Q

Q

M

M

V

M

I

V

S

A

L

L

A

E

P

P

L

V

H

R

K

Q

E

K

F

W

N

G

I

L

K

D

R

R

V

I

E

I

V

V

A

S

T

T

Y

Y

Q

Q

A

A

V

V

S
|
S

G
|
G

A

A

G
|
G

K
x
M

K

G

G

A

M

I

E

L

E

E

L

T

F

Q

E

R

Q

E

M

L

R

R

A

E

I

V

F

L

N

N

F

D

K

G

L

V

D

K

K

P

V

C

V

D

D

L

E

H

R

A

E

E

V

I

F

L

-

P

-

S

-

G

-

D

-

K

Q

H

Y

Y

Q

P

I

I

A

A

L

F

N

N

V

A

I

L

P

P

H

Q

I

I

D

D

R

V

F

F

M

T

D

D

N

N

G

D

F

Y

T

T

R

Y

E
|
E

E

E

M

M

K
|
K

M

M

V

T

N

K

E

E

T

T

N

K

K

K

I

I

M

M

H

E

S

D

N

D

-

S

I

I

E

A

V

V

A

S

P

A

T

T

C

C

V

V

R
|
R

V

I

P

P

V

V

L

L

R

S

S

A

H

H

S

S

E

E

A

S

I

V

T

Y

I

I

H

E

C

T

E

K

K

E

E

V

V

A

D

P

V

I

K

E

R

E

A

V

R

K

E

A

V

A

L

I

E

A

S

A

F

F

E

P

D

G

I

A

K

V

V

L

I

E

D

D

N

D

P

V

E

A

K

H

N

Q

E

I

Y

Y

P

P

M

Q

P

A

I

I

I

N

A

A

T

V

D

G

T

S

D

R

Y

D

V

T

Y

F

V

V

G

G

R

R

I

I

R

R

K

K

D

D

L

L

F

D

D

A

N

E

K

K

T

G

L

I

H

H

F

M

F

W

N

V

V

V

A

S

D

D

Q
x
N

L
|
L

R

L

V

K

G

G

A

A

T

W

N

N

A

S

V

V

R

Q

I

I

A

A

binding 2-aminobenzene-1,4-dicarboxylic acid: R98 (= R100), G158 (= G162), E219 (= E216), K222 (= K219), R244 (= R240)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G8), T10 (= T10), G11 (= G11), A12 (= A12), V13 (= V13), A35 (= A35), S36 (= S36), R38 (= R38), S39 (= S39), T56 (≠ L56), S70 (= S72), A71 (= A73), G72 (= G74), T75 (≠ V77), C127 (= C131), S157 (= S161), G158 (= G162), G160 (= G164), M161 (≠ K165), N324 (≠ Q320), L325 (= L321)

3tz6A Crystal structure of aspartate semialdehyde dehydrogenase complexed with inhibitor smcs (cys) and phosphate from mycobacterium tuberculosis h37rv (see paper)
40% identity, 95% coverage: 2:333/350 of query aligns to 2:337/342 of 3tz6A

query
sites
3tz6A

Y

L

N

S

V

I

A

G

I

I

V

V

G

G

A

A

T

T

G

G

A

Q

V

V

G

G

E

Q

E

V

L

M

F

R

R

T

V

L

L

L

E

D

E

E

Y

R

N

D

F

F

P

P

V

A

K

S

K

A

L

V

V

R

P

F

L

F

A

A

S

S

A

A

R

R

S

S

V

Q

G

G

K

R

E

K

V

L

E

A

F

F

K

R

G

G

E

Q

T

E

L

I

K

E

V

V

K

E

E

D

L

-

T

-

C

A

D

E

V

T

F

A

E

D

K

P

E

S

E

G

I

L

D

D

I

I

A

A

F

L

F

F

S

S

A

A

G

G

G

S

S

A

V

M

S

S

A

K

K

V

F

Q

A

A

P

P

C

R

A

F

V

A

K

A

A

A

G

G

A

V

V

T

V

V

I

I

D

D

N

N

T

S

S

S

Y

A

F

W

R
|
R

M

K

D

D

K

P

D

D

V

V

P

P

L

L

V

V

P
x
S

E

E

V

V

N

N

P

F

E

E

D

R

I

D

A

A

L
x
H

W

R

R
|
R

K

P

K

K

G

G

I

I

A

A

N

N

P

P

N
|
N

C
|
C

S

T

T

T

I

M

Q

A

M

A

V

M

I

P

S

V

L

L

A

K

P

V

L

L

H

H

K

D

E

E

F

A

N

R

I

L

K

V

R

R

V

L

E

V

V

V

A

S

T

S

Y

Y

Q
|
Q

A

A

V

V

S

S

G
|
G

A

S

G

G

K

L

K

A

G

G

M

V

E

A

E

E

L

L

F

A

E

E

Q

Q

M

A

R

R

A

A

I

V

F

I

N

G

F

G

K

A

L

E

D

Q

K

L

V

V

V

Y

D

D

E
x
G

R

G

E

A

V

L

-

E

-
x
F

-

P

-

N

-

T

F

Y

Q

V

Y

A

Q
x
P

I

I

A

A

L

F

N

N

V

V

I

V

P

P

H

L

I

A

D

G

R

S

F

L

M

V

D

D

N

D

G

G

F

S

-

G

-

E

T

T

R

D

E
|
E

E

D

M
x
Q

K
|
K

M

L

V
x
R

N
x
F

E

E

T

S

N

R

K

K

I

I

M

L

H

G

-

I

S

P

N

D

I

L

E

L

V

V

A

S

P

G

T

T

C

C

V

V

R
|
R

V

V

P

P

V

V

L

F

R

T

S

G

H
|
H

S

S

E

L

A

S

I

I

T

N

I

A

H

E

C

F

E

A

K

Q

E

P

V

L

D

S

V

P

K
x
E

R

R

A

A

R
|
R

E

E

V

L

L

L

E

D

S

G

F

A

E

T

D

G

I

V

K

Q

V

L

I

V

D

D

N

-

P

-

E

-

K

-

N

-

E

-

Y

V

P

P

M

T

P

P

I

L

I

A

A

A

T

A

D

G

T

V

D

D

Y

E

V

S

Y

L

V

V

G

G

R

R

I

I

R

R

K

R

D

D

-

P

-

G

L

V

F

P

D

D

N

G

K

R

T

G

L

L

H

A

F

L

F

F

N

V

V

S

A

G

D

D

Q

N

L

L

R

R

V

K

G

G

A

A

T

L

N

N

A

T

V

I

R
x
Q

I

I

A

A

active site: C129 (= C131), Q156 (= Q158), R248 (= R240), H255 (= H247)
binding cysteine: C129 (= C131), Q156 (= Q158), G160 (= G162), E223 (= E216), R248 (= R240), H255 (= H247)
binding glycerol: S108 (≠ P110), G187 (≠ E189), F192 (vs. gap), P201 (≠ Q196), Q225 (≠ M218), R228 (≠ V221), F229 (≠ N222), Q335 (≠ R331)
binding sulfate ion: R98 (= R100), H117 (≠ L119), R119 (= R121), N128 (= N130), C129 (= C131), K226 (= K219), E270 (≠ K262), R273 (= R265)

Sites not aligning to the query:

binding glycerol: 338, 339

Query Sequence

>WP_007472841.1 NCBI__GCF_000170735.1:WP_007472841.1
MYNVAIVGATGAVGEELFRVLEEYNFPVKKLVPLASARSVGKEVEFKGETLKVKELTCDV
FEKEEIDIAFFSAGGSVSAKFAPCAVKAGAVVIDNTSYFRMDKDVPLVVPEVNPEDIALW
RKKGIIANPNCSTIQMVISLAPLHKEFNIKRVEVATYQAVSGAGKKGMEELFEQMRAIFN
FKLDKVVDEREVFQYQIALNVIPHIDRFMDNGFTREEMKMVNETNKIMHSNIEVAPTCVR
VPVLRSHSEAITIHCEKEVDVKRAREVLESFEDIKVIDNPEKNEYPMPIIATDTDYVYVG
RIRKDLFDNKTLHFFNVADQLRVGAATNAVRIALKWIEMEGEKFLKEKDK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory