SitesBLAST
Comparing WP_007472875.1 NCBI__GCF_000170735.1:WP_007472875.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
P04805 Glutamate--tRNA ligase; Glutamyl-tRNA synthetase; GluRS; EC 6.1.1.17 from Escherichia coli (strain K12) (see 4 papers)
36% identity, 81% coverage: 3:354/433 of query aligns to 6:367/471 of P04805
- C98 (= C94) mutation to S: 10-fold decrease in activity. Strong decrease in zinc content.
- C100 (= C96) mutation to S: Loss of activity. Strong decrease in zinc content.; mutation to Y: Does not prevent zinc binding. Reduces only 2-fold the binding affinity for tRNA(Glu), but reduces more than 10-fold the affinity for glutamate in the presence of tRNA(Glu).
- C125 (= C121) mutation to S: Loss of activity. Strong decrease in zinc content.
- H127 (≠ S123) mutation to Q: 10-fold decrease in activity. Strong decrease in zinc content.
- H129 (≠ S125) mutation to Q: No change in activity or in zinc content.
- H131 (≠ K127) mutation to Q: No change in activity or in zinc content.
- H132 (≠ E128) mutation to Q: No change in activity or in zinc content.
- C138 (≠ F139) mutation to S: No change in activity or in zinc content.
- S239 (= S240) modified: Phosphoserine; mutation to D: Does not aminoacylate tRNA(Glu), not phosphorylated by HipA.
8i9iA Glutamyl-tRNA synthetase from escherichia coli bound to glutamate and zinc
35% identity, 81% coverage: 3:354/433 of query aligns to 6:367/468 of 8i9iA
8vc5A Crystal structure of glutamyl-tRNA synthetase glurs from pseudomonas aeruginosa (zinc bound)
37% identity, 73% coverage: 3:320/433 of query aligns to 7:329/488 of 8vc5A
2cv2A Glutamyl-tRNA synthetase from thermus thermophilus in complex with tRNA(glu) and an enzyme inhibitor, glu-ams (see paper)
31% identity, 94% coverage: 1:409/433 of query aligns to 3:441/468 of 2cv2A
- active site: K246 (= K241)
- binding o5'-(l-glutamyl-sulfamoyl)-adenosine: R5 (= R3), A7 (= A5), S9 (= S7), G17 (= G15), I21 (≠ V19), E41 (= E39), Y187 (= Y181), R205 (= R199), A206 (≠ G200), E208 (≠ D202), W209 (≠ H203), L235 (≠ I230), L236 (≠ I231)
- binding : S9 (= S7), T43 (= T41), D44 (= D42), R47 (= R45), V145 (= V140), R163 (≠ K157), Y168 (≠ F162), E172 (= E166), V177 (= V171), K180 (≠ R174), S181 (≠ A175), Y187 (= Y181), E207 (= E201), E208 (≠ D202), W209 (≠ H203), V211 (≠ S205), R237 (≠ L232), K241 (≠ G236), L272 (= L267), M273 (≠ L268), G274 (= G269), E282 (= E275), S299 (= S292), P303 (≠ A296), V304 (≠ K297), K309 (= K302), W312 (≠ F305), R319 (≠ K312), P357 (≠ D336), R358 (≠ E337), R417 (≠ N385), Q432 (≠ M400), R435 (= R403)
Sites not aligning to the query:
2cv1A Glutamyl-tRNA synthetase from thermus thermophilus in complex with tRNA(glu), atp, and an analog of l-glutamate: a quaternary complex
31% identity, 94% coverage: 1:409/433 of query aligns to 3:441/468 of 2cv1A
- active site: K246 (= K241)
- binding adenosine-5'-triphosphate: P8 (= P6), S9 (= S7), G17 (= G15), T18 (≠ N16), I21 (≠ V19), R47 (= R45), A206 (≠ G200), W209 (≠ H203), L235 (≠ I230), L236 (≠ I231)
- binding (4s)-4-amino-5-hydroxypentanoic acid: R5 (= R3), A7 (= A5), E41 (= E39), Y187 (= Y181), R205 (= R199), W209 (≠ H203)
- binding : S9 (= S7), E41 (= E39), T43 (= T41), D44 (= D42), R47 (= R45), V145 (= V140), R163 (≠ K157), V166 (≠ M160), E172 (= E166), V177 (= V171), K180 (≠ R174), S181 (≠ A175), Y187 (= Y181), E207 (= E201), E208 (≠ D202), W209 (≠ H203), V211 (≠ S205), R237 (≠ L232), K241 (≠ G236), K243 (= K238), M273 (≠ L268), G274 (= G269), S276 (= S271), E282 (= E275), S299 (= S292), P303 (≠ A296), V304 (≠ K297), K309 (= K302), W312 (≠ F305), R319 (≠ K312), P357 (≠ D336), R358 (≠ E337), R417 (≠ N385), L427 (≠ G395), Q432 (≠ M400), R435 (= R403)
Sites not aligning to the query:
2cuzA Glutamyl-tRNA synthetase from thermus thermophilus in complex with l- glutamate (see paper)
31% identity, 94% coverage: 1:409/433 of query aligns to 3:441/468 of 2cuzA
1n78A Crystal structure of thermus thermophilus glutamyl-tRNA synthetase complexed with tRNA(glu) and glutamol-amp. (see paper)
31% identity, 94% coverage: 1:409/433 of query aligns to 3:441/468 of 1n78A
- active site: K246 (= K241)
- binding glutamol-amp: R5 (= R3), A7 (= A5), P8 (= P6), S9 (= S7), G17 (= G15), T18 (≠ N16), I21 (≠ V19), E41 (= E39), Y187 (= Y181), N191 (≠ C185), R205 (= R199), A206 (≠ G200), E208 (≠ D202), W209 (≠ H203), L235 (≠ I230), L236 (≠ I231)
- binding : S9 (= S7), T43 (= T41), D44 (= D42), R47 (= R45), V145 (= V140), R163 (≠ K157), V166 (≠ M160), Y168 (≠ F162), E172 (= E166), V177 (= V171), K180 (≠ R174), S181 (≠ A175), Y187 (= Y181), E207 (= E201), E208 (≠ D202), W209 (≠ H203), L210 (= L204), V211 (≠ S205), R237 (≠ L232), K241 (≠ G236), M273 (≠ L268), G274 (= G269), E282 (= E275), R297 (≠ K290), P303 (≠ A296), V304 (≠ K297), K309 (= K302), W312 (≠ F305), R319 (≠ K312), P357 (≠ D336), R358 (≠ E337), R417 (≠ N385), L427 (≠ G395), Q432 (≠ M400), R435 (= R403)
Sites not aligning to the query:
1j09A Crystal structure of thermus thermophilus glutamyl-tRNA synthetase complexed with atp and glu (see paper)
31% identity, 94% coverage: 1:409/433 of query aligns to 3:441/468 of 1j09A
- active site: K246 (= K241)
- binding adenosine-5'-triphosphate: H15 (= H13), E208 (≠ D202), L235 (≠ I230), L236 (≠ I231), K243 (= K238), I244 (≠ L239), S245 (= S240), K246 (= K241), R247 (= R242)
- binding glutamic acid: R5 (= R3), A7 (= A5), S9 (= S7), E41 (= E39), Y187 (= Y181), N191 (≠ C185), R205 (= R199), W209 (≠ H203)
P27000 Glutamate--tRNA ligase; Glutamyl-tRNA synthetase; GluRS; EC 6.1.1.17 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see paper)
31% identity, 94% coverage: 1:409/433 of query aligns to 3:441/468 of P27000
- R358 (≠ E337) mutation to Q: Reduces affinity for tRNA and abolishes the ability to discriminate between tRNA(Glu) and tRNA(Gln).
1g59A Glutamyl-tRNA synthetase complexed with tRNA(glu). (see paper)
31% identity, 94% coverage: 1:409/433 of query aligns to 3:441/468 of 1g59A
- binding : D44 (= D42), R45 (≠ K43), A46 (≠ E44), R47 (= R45), P109 (≠ E98), V145 (= V140), R163 (≠ K157), V166 (≠ M160), E172 (= E166), V177 (= V171), K180 (≠ R174), S181 (≠ A175), D182 (= D176), E207 (= E201), E208 (≠ D202), R237 (≠ L232), K241 (≠ G236), T242 (≠ K237), K243 (= K238), M273 (≠ L268), G274 (= G269), E282 (= E275), S299 (= S292), L300 (≠ K293), P303 (≠ A296), V304 (≠ K297), K309 (= K302), W312 (≠ F305), R319 (≠ K312), P357 (≠ D336), R358 (≠ E337), R417 (≠ N385), K426 (= K394), L427 (≠ G395), Q432 (≠ M400), R435 (= R403)
Sites not aligning to the query:
3al0C Crystal structure of the glutamine transamidosome from thermotoga maritima in the glutamylation state. (see paper)
33% identity, 98% coverage: 2:424/433 of query aligns to 105:528/564 of 3al0C
- active site: S110 (= S7), K335 (= K241)
- binding o5'-(l-glutamyl-sulfamoyl)-adenosine: R106 (= R3), A108 (= A5), P109 (= P6), G118 (= G15), T122 (≠ V19), E142 (= E39), Y276 (= Y181), R294 (= R199), G295 (= G200), D297 (= D202), H298 (= H203), L324 (≠ I230), I325 (= I231), L333 (= L239)
- binding : T144 (= T41), D145 (= D42), R148 (= R45), Y208 (≠ C96), P213 (≠ L101), K252 (= K157), M255 (= M160), I266 (≠ V171), K269 (≠ R174), S270 (≠ A175), Y276 (= Y181), D297 (= D202), H298 (= H203), L299 (= L204), S300 (= S205), N301 (= N206), K304 (= K209), R330 (≠ G236), P332 (≠ K238), G363 (= G269), W364 (≠ N270), R365 (≠ S271), E370 (= E275), S387 (= S292), K389 (≠ S294), V391 (≠ A296), I392 (≠ K297), K397 (= K302), W400 (≠ F305), R407 (≠ K312), E446 (≠ D336), K447 (≠ E337), Q453 (≠ E343), I457 (≠ K347), R509 (= R403), K520 (= K416), Q524 (≠ K420), R527 (= R423)
Sites not aligning to the query:
Q8DLI5 Glutamate--tRNA ligase; Glutamyl-tRNA synthetase; GluRS; EC 6.1.1.17 from Thermosynechococcus vestitus (strain NIES-2133 / IAM M-273 / BP-1) (see paper)
37% identity, 73% coverage: 2:315/433 of query aligns to 5:328/485 of Q8DLI5
- R6 (= R3) binding L-glutamate
- Y192 (= Y181) binding L-glutamate
2cfoA Non-discriminating glutamyl-tRNA synthetase from thermosynechococcus elongatus in complex with glu (see paper)
37% identity, 73% coverage: 2:315/433 of query aligns to 4:327/484 of 2cfoA
4griB Crystal structure of a glutamyl-tRNA synthetase glurs from borrelia burgdorferi bound to glutamic acid and zinc (see paper)
38% identity, 72% coverage: 2:312/433 of query aligns to 4:326/485 of 4griB
- active site: S9 (= S7), K253 (= K241)
- binding glutamic acid: R5 (= R3), A7 (= A5), S9 (= S7), E41 (= E39), Y194 (= Y181), R212 (= R199), W216 (≠ H203)
- binding zinc ion: C105 (= C94), C107 (= C96), Y128 (= Y117), C132 (= C121)
4g6zA Crystal structure of a glutamyl-tRNA synthetase glurs from burkholderia thailandensis bound to l-glutamate (see paper)
35% identity, 79% coverage: 3:346/433 of query aligns to 6:347/380 of 4g6zA
6brlA Crystal structure of a glutamate tRNA ligase from elizabethkingia meningosepticum ccug26117 in complex with its amino acid (see paper)
31% identity, 96% coverage: 2:418/433 of query aligns to 5:481/502 of 6brlA
4a91A Crystal structure of the glutamyl-queuosine trnaasp synthetase from e. Coli complexed with l-glutamate (see paper)
31% identity, 55% coverage: 3:242/433 of query aligns to 7:230/290 of 4a91A
- active site: S11 (= S7), K229 (= K241)
- binding glutamic acid: R7 (= R3), A9 (= A5), S11 (= S7), E43 (= E39), Y170 (= Y181), R188 (= R199), L192 (≠ H203)
- binding zinc ion: C99 (= C94), C101 (= C96), Y113 (= Y117), C117 (= C121)
P27305 Glutamyl-Q tRNA(Asp) synthetase; Glu-Q-RSs; EC 6.1.1.- from Escherichia coli (strain K12) (see paper)
31% identity, 55% coverage: 3:242/433 of query aligns to 19:242/308 of P27305
- E55 (= E39) binding L-glutamate
- Y182 (= Y181) binding L-glutamate
- R200 (= R199) binding L-glutamate
3aiiA Archaeal non-discriminating glutamyl-tRNA synthetase from methanothermobacter thermautotrophicus (see paper)
28% identity, 62% coverage: 1:269/433 of query aligns to 12:278/455 of 3aiiA
5zdkA Crystal structure analysis of ttqrs in complex with atp (see paper)
29% identity, 45% coverage: 1:195/433 of query aligns to 26:218/543 of 5zdkA
Sites not aligning to the query:
Query Sequence
>WP_007472875.1 NCBI__GCF_000170735.1:WP_007472875.1
MLRFAPSPTGDMHVGNLRVAIFNYIVAKQRGEKFLIRIEDTDKERNIEGKDKEILDILNL
CGINYDKVVYQSNNFHIHQNLAKKLLEENKAFVCFCDEEELNFQREKAKIEKRPYRYSGK
CESLSKKEVEKLMREKKEFVVRIKKPTADIKFIDEIKGKMQFTPFEVDSFVILRADYTPT
YNFACSVDDMLYDISLVIRGEDHLSNTPKQIHIRNLLGYDKEIKYAHLPIILNEEGKKLS
KRERHSSVKYLLKEGYLPQAIVNYLILLGNSFKREIFTLDEAIEFFDLSKISKSPAKFDI
EKLKFINKAHMKKIENIAEFLGYHKNYNELLEIFRDESDTLKEIKEKFERIFEECEDEEF
KNEREILKKEILNNELEEEYNDFKNRISKNTGLKGKKLFMPLRELLINQKHGAEIKDLYK
AMRGYLKDIIKKR
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory