SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_007473461.1 NCBI__GCF_000170735.1:WP_007473461.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P05654 Aspartate carbamoyltransferase catalytic subunit; Aspartate transcarbamylase; ATCase; EC 2.1.3.2 from Bacillus subtilis (strain 168) (see paper)
42% identity, 98% coverage: 3:286/291 of query aligns to 2:286/304 of P05654

query
sites
P05654

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Sites not aligning to the query:

303 modified: Phosphoserine

3r7fA Crystal structure of cp-bound aspartate transcarbamoylase from bacillus subtilis (see paper)
42% identity, 98% coverage: 3:286/291 of query aligns to 2:286/291 of 3r7fA

query
sites
3r7fA

K

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active site: R49 (= R50), T50 (= T51), K77 (= K78), R99 (= R100), H127 (= H128), Q130 (= Q131), L210 (= L210), P249 (= P249), G277 (= G277)
binding phosphoric acid mono(formamide)ester: S47 (= S48), T48 (= T49), R49 (= R50), T50 (= T51), R99 (= R100), H127 (= H128), Q130 (= Q131), P249 (= P249), A250 (≠ G250)
binding phosphate ion: S11 (= S12), T12 (≠ K13), Q23 (≠ K24), K26 (≠ L27), E140 (≠ Q141), R171 (= R172), K241 (≠ D241), H243 (≠ D243), K272 (≠ N272), K272 (≠ N272), K275 (= K275)

3r7dA Crystal structure of unliganded aspartate transcarbamoylase from bacillus subtilis (see paper)
42% identity, 98% coverage: 3:286/291 of query aligns to 2:286/291 of 3r7dA

query
sites
3r7dA

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active site: R49 (= R50), T50 (= T51), K77 (= K78), R99 (= R100), H127 (= H128), Q130 (= Q131), L210 (= L210), P249 (= P249), G277 (= G277)
binding phosphate ion: S11 (= S12), T12 (≠ K13), T73 (≠ S74), S74 (= S75), K77 (= K78), R171 (= R172)

3r7lA Crystal structure of pala-bound aspartate transcarbamoylase from bacillus subtilis (see paper)
42% identity, 98% coverage: 3:286/291 of query aligns to 2:286/290 of 3r7lA

query
sites
3r7lA

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active site: R49 (= R50), T50 (= T51), K77 (= K78), R99 (= R100), H127 (= H128), Q130 (= Q131), L210 (= L210), P249 (= P249), G277 (= G277)
binding n-(phosphonacetyl)-l-aspartic acid: S47 (= S48), T48 (= T49), R49 (= R50), T50 (= T51), S74 (= S75), K77 (= K78), R99 (= R100), H127 (= H128), R160 (= R161), R211 (= R211), Q213 (= Q213), A250 (≠ G250)

4bjhB Crystal structure of the aquifex reactor complex formed by dihydroorotase (h180a, h232a) with dihydroorotate and aspartate transcarbamoylase with n-(phosphonacetyl)-l-aspartate (pala) (see paper)
39% identity, 98% coverage: 3:288/291 of query aligns to 2:289/291 of 4bjhB

query
sites
4bjhB

K

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L

A

I

V

I

L

N

-

I

F

F

F

F

S

E

E

N

P

S
|
S

T
|
T

R
|
R

T
|
T

R

R

S

L

S

S

F

F

E

E

I

K

A

A

K

R

R

E

L

L

S

G

A

I

D

E

V

T

V

Y

N

L

L

V

D

S

V

G

N

S

R

E

S

S

T

T

A

V

K
|
K

G

G

E

E

T

S

L

F

F

F

D

D

T

T

A

L

A

K

N

T

L

F

D

E

A

G

M

L

G

G

P

F

D

D

A

Y

I

V

V

F

R
|
R

H

V

N

P

R

F

S

V

G

F

V

F

P

P

-

Y

K

K

I

E

L

I

S

V

K

K

Y

S

V

L

K

N

C

L

S

R

I

L

I

V

N

N

A

A

G

G

D

D

G

G

A

T

H

H

Q

Q

H
|
H

P

P

T

S

Q
|
Q

G

G

L

L

L

I

D

D

L

F

F

F

T

T

L

I

L

K

Q

E

K

H

F

F

K

G

T

E

L

V

E

K

G

D

K

L

T

R

I

V

A

L

I

Y

V

V

G

G

D

D

I

I

R

K

N

H

S

S

R
|
R

V
|
V

A

F

N

R

S

S

D

G

I

A

E

P

L

L

F

L

T

N

R

M

F

F

G

G

A

A

N

K

V

I

I

G

L

V

V

C

G

G

P

P

P

K

Q

T

F

L

L

I

P

P

K

R

T

D

T

V

-

E

-

V

-

F

-

K

L

V

P

D

T

V

T

F

Y

D

N

D

L

V

K

D

E

K

A

G

I

I

N

D

H

W

S

A

D

D

A

V

I

V

I

I

S

W

L

L

R
|
R

T

L

Q
|
Q

T

K

E

E

R

R

H

Q

K

K

T

E

P

N

T

Y

F

I

A

P

S

S

L

E

K

S

D

S

Y

Y

A

F

E

K

N

Q

F

F

K

G

I

L

T

T

K

K

E

E

V

R

I

F

K

-

D

E

K

K

D

V

I

K

L

L

I

Y

M

M

H

H

P

P

G
|
G

P

P

V

V

H

N

R

R

N

N

I

V

D

D

I

I

D

D

D

H

E

E

V

L

L

V

E

Y

D

T

E

E

R

K

C

S

L

L

V

I

L

Q

N

E

Q

Q

V

V

K

K

N

N

G

G

V

I

A

P

V

V

R

R

M

K

G

A

I

I

L

Y

K

K

F

F

L

L

active site: R47 (= R50), T48 (= T51), K75 (= K78), R97 (= R100), H126 (= H128), Q129 (= Q131)
binding n-(phosphonacetyl)-l-aspartic acid: S45 (= S48), T46 (= T49), R47 (= R50), T48 (= T51), R97 (= R100), H126 (= H128), R159 (= R161), V160 (= V162), R213 (= R211), Q215 (= Q213), G251 (= G250)

3d6nB Crystal structure of aquifex dihydroorotase activated by aspartate transcarbamoylase (see paper)
39% identity, 98% coverage: 3:288/291 of query aligns to 2:289/291 of 3d6nB

query
sites
3d6nB

K

R

H

S

L

L

T

I

H

S

T

S

K

L

D

D

L

L

S

T

K

R

N

E

E

E

I

V

V

E

T

E

I

I

F

L

K

K

Y

Y

A

A

K

K

E

E

F

F

L

-

D

K

E

E

K

G

K

K

R

E

D

E

D

T

L

I

R

K

G

A

K

S

L

A

I

V

I

L

N

-

I

F

F

F

F

S

E

E

N

P

S

S

T

T

R
|
R

T
|
T

R

R

S

L

S

S

F

F

E

E

I

K

A

A

K

R

R

E

L

L

S

G

A

I

D

E

V

T

V

Y

N

L

L

V

D

S

V

G

N

S

R

E

S

S

T

T

A

V

K
|
K

G

G

E

E

T

S

L

F

F

F

D

D

T

T

A

L

A

K

N

T

L

F

D

E

A

G

M

L

G

G

P

F

D

D

A

Y

I

V

V

F

R
|
R

H

V

N

P

R

F

S

V

G

F

V

F

P

P

-

Y

K

K

I

E

L

I

S

V

K

K

Y

S

V

L

K

N

C

L

S

R

I

L

I

V

N

N

A

A

G

G

D

D

G

G

A

T

H

H

Q

Q

H
|
H

P

P

T

S

Q
|
Q

G

G

L

L

L

I

D

D

L

F

F

F

T

T

L

I

L

K

Q

E

K

H

F

F

K

G

T

E

L

V

E

K

G

D

K

L

T

R

I

V

A

L

I

Y

V

V

G

G

D

D

I

I

R

K

N

H

S

S

R
|
R

V
|
V

A

F

N

R

S

S

D

G

I

A

E

P

L

L

F

L

T

N

R

M

F

F

G

G

A

A

N

K

V

I

I

G

L

V

V

C

G

G

P

P

P

K

Q

T

F

L

L

I

P

P

K

R

T

D

T

V

-

E

-

V

-

F

-

K

L

V

P

D

T

V

T

F

Y

D

N

D

L

V

K

D

E

K

A

G

I

I

N

D

H

W

S

A

D

D

A

V

I

V

I

I

S

W

L

L

R
|
R

T

L

Q

Q

T

K

E

E

R

R

H

Q

K

K

T

E

P

N

T

Y

F

I

A

P

S

S

L

E

K

S

D

S

Y

Y

A

F

E

K

N

Q

F

F

K

G

I

L

T

T

K

K

E

E

V

R

I

F

K

-

D

E

K

K

D

V

I

K

L

L

I

Y

M

M

H

H

P

P

G
|
G

P

P

V

V

H

N

R

R

N

N

I

V

D

D

I

I

D

D

D

H

E

E

V

L

L

V

E

Y

D

T

E

E

R

K

C

S

L

L

V

I

L

Q

N

E

Q

Q

V

V

K

K

N

N

G

G

V

I

A

P

V

V

R

R

M

K

G

A

I

I

L

Y

K

K

F

F

L

L

active site: R47 (= R50), T48 (= T51), K75 (= K78), R97 (= R100), H126 (= H128), Q129 (= Q131)
binding citrate anion: T48 (= T51), R97 (= R100), H126 (= H128), R159 (= R161), V160 (= V162), R213 (= R211), G251 (= G250)

6pnzA The structure of the aspartate transcarbamoylase trimer from staphylococcus aureus complexed with pala at 2.27 resolution.
35% identity, 99% coverage: 4:290/291 of query aligns to 3:293/293 of 6pnzA

query
sites
6pnzA

H

H

L

L

T

L

H

S

T

M

K

E

D

H

L

L

S

S

K

T

N

D

E

Q

I

I

V

Y

T

K

I

L

F

I

K

Q

Y

K

A

A

K

S

E

Q

F

F

L

K

D

S

-

G

E

E

K

R

K

Q

R

L

D

P

D

N

L

F

R

E

G

G

K

K

L

Y

I

V

I

A

N

N

I

L

F

F

E

E

N

N

S
|
S

T
|
T

R
|
R

T
|
T

R

K

S

C

S

S

F

F

E

E

I

M

A

A

A

E

K

L

R

K

L

L

S

G

A

L

D

K

V

T

V

I

N

S

L

F

D

E

V

T

N

S

R

T

S

S

S
|
S

T

V

A

S

K
|
K

G

G

E

E

T

S

L

L

F

Y

D

D

T

T

A

C

A

K

N

T

L

L

D

E

A

S

M

I

G

G

P

C

D

D

A

L

I

L

I

V

V

I

R
|
R

H

H

N

P

R

F

S

N

G

N

V

Y

P

Y

K

E

I

K

L

L

S

A

K

N

Y

-

V

I

K

N

C

I

S

P

I

I

I

A

N

N

A

A

G

G

D

D

G

G

A

S

H

G

Q

Q

H
|
H

P

P

T

T

Q

Q

G

S

L

L

D

D

L

L

F

M

T

T

L

I

L

Y

Q

E

K

E

F

Y

K

G

T

Y

L

F

E

E

G

G

K

L

T

N

I

V

A

L

I

I

V

C

G

G

D

D

I

I

R

K

N

N

S

S

R
|
R

V

V

A

A

N

R

S

S

D

N

I

Y

E

H

L

S

F

L

T

K

R

A

F

L

G

G

A

A

N

N

V

V

I

M

L

F

V

N

G

S

P

P

P

N

Q

A

F

W

L

I

P

D

K

D

T

S

T

L

L

E

P

A

T

P

T

Y

Y

V

N

N

L

I

K

D

E

D

A

V

I

I

N

E

H

T

S

V

D

D

A

I

I

V

I

M

S

L

L

L

R
|
R

T

I

Q
|
Q

T

H

E

E

R

R

H

H

-

G

-

L

-

A

K

E

T

E

P

T

T

R

F

F

A

A

S

A

L

-

K

D

D

D

Y

Y

A

H

E

Q

N

K

F

H

K

G

I

L

T

N

K

-

E

E

V

V

I

R

K

Y

D

N

K

K

-

L

-

Q

-

E

D

H

I

A

L

I

I

V

M

M

H

H

P

P

G
x
A

P

P

V

V

H

N

R

R

N

G

I

V

D

E

I

I

D

Q

D

S

E

D

V

L

L

V

E

E

D

A

E

S

R

K

C

S

L

R

V

I

L

F

N

K

Q

Q

V

M

K

E

N

N

G

G

V

V

A

Y

V

L

R

R

M

M

G

A

I

V

L

I

K

D

F

E

L

L

I

L

E

K

binding n-(phosphonacetyl)-l-aspartic acid: S48 (= S48), T49 (= T49), R50 (= R50), T51 (= T51), S75 (= S75), K78 (= K78), R100 (= R100), H127 (= H128), R160 (= R161), R210 (= R211), Q212 (= Q213), A253 (≠ G250)

4eknB Structure of the catalytic chain of methanococcus jannaschii aspartate transcarbamoylase in a hexagonal crystal form (see paper)
36% identity, 99% coverage: 3:291/291 of query aligns to 2:302/304 of 4eknB

query
sites
4eknB

K

K

H

H

L

L

T

I

H

S

T

M

K

K

D

D

L

I

S

G

K

K

N

E

E

E

I

I

V

L

T

E

I

I

F

L

K

D

Y

E

A

A

K

R

-

K

-

M

-

E

E

E

F

L

L

L

D

N

E

T

K

K

K

R

R

P

D

L

D

K

L

L

-

L

R

E

G

G

K

K

L

I

I

L

I

A

N

T

I

V

F

F

F

Y

E

E

N

P

S

S

T

T

R

R

T

T

R

R

S

L

S

S

F

F

E

E

I

T

A

A

A

M

K

K

R

R

L

L

S

G

A

G

D

E

V

V

V

I

N

T

L

M

-

T

D

D

V

L

N

K

R

S

S

S

T

V

A

A

K

K

G

G

E

E

T

S

L

L

F

I

D

D

T

T

A

I

A

R

N

V

L

I

D

S

A

G

M

Y

G

A

P

-

D

D

A

I

I

I

I

V

V

L

R

R

H

H

N

P

R

S

S

E

G

G

V

A

P

A

K

R

I

L

L

A

S

S

K

E

Y

Y

V

S

K

Q

C

V

S

P

I

I

N

N

A

A

G

G

D

D

G

G

A

S

H

N

Q

Q

H

H

P

P

T

T

Q

Q

G

T

L

L

D

D

L

L

F

Y

T

T

L

I

L

M

Q

R

K

E

F

I

K

G

T

R

L

I

E

D

G

G

K

I

T

K

I

I

A

A

I

F

V

V

G

G

D

D

I

L

R

K

N

Y

S
x
G

R
|
R

V
x
T

A

V

N

H

S

S

D

L

I

V

E

Y

L

A

F

L

T

S

R

L

F

F

-

E

G

N

A

V

N

E

V

M

I

Y

L

F

V

V

G

S

P

P

P

K

Q

E

F

L

-

R

L

L

P

P

K

K

T

D

T

I

L

I

P

E

-

D

-

L

T

K

T

A

Y

K

N

N

L

I

K

K

E

F

A

Y

I

E

N

K

H

E

S

S

D

L

A

D

I

D

I

L

S

D

L

D

-

D

-

I

-

D

-

V

-

L

-

Y

-

V

-
x
T

R
|
R

T

I

Q

Q

T

K

E

E

R

R

H

F

K

P

T

D

P

P

T

N

-

E

F

Y

A

E

S

K

L

V

K

K

D

G

Y

-

A

-

E

-

N

S

F

Y

K

K

I

I

T

K

K

R

E

E

V

Y

I

V

K

E

D

G

K

K

D

K

I

F

L

I

I

I

M

M

H

H

P
|
P

G

L

P

P

V

R

H

V

R

D

N

E

I

I

D

D

I

Y

D

D

D

V

E

D

V

D

L

L

E

P

D

Q

E

A

R

K

C

Y

L

-

V

-

L

F

N

K

Q

Q

V

S

K

F

N

Y

G
|
G

V

I

A

P

V

V

R

R

M

M

G

A

I

I

L

L

K

K

F

K

L

L

I

I

E

E

N

D

active site: T225 (vs. gap), P262 (= P249), G288 (= G277)
binding sulfate ion: G163 (≠ S160), R164 (= R161), T165 (≠ V162), R226 (= R211)

5g1nE Aspartate transcarbamoylase domain of human cad bound to pala (see paper)
31% identity, 98% coverage: 1:285/291 of query aligns to 4:300/307 of 5g1nE

query
sites
5g1nE

M

V

S

G

K

Q

H

H

L

I

T

L

H

S

T

V

K

Q

D

Q

L

F

S

T

K

K

N

D

E

Q

I

M

V

S

T

H

I

L

F

F

K

N

Y

V

A

A

K

H

-

T

-

L

E

R

F

M

L

M

D

V

E

Q

K

K

K

E

R

R

-

S

-

L

D

D

D

I

L

L

R

K

G

G

K

K

L

V

I

M

I

A

N

S

I

M

F

F

F

Y

E

E

N

V

S
|
S

T
|
T

R
|
R

T
|
T

R

S

S

S

F

F

E

A

I

A

A

A

A

M

K

A

R

R

L

L

S

G

A

G

D

A

V

V

V

L

N

S

L

F

D

S

V

E

N

A

R

T

S

S

S
|
S

T

V

A

Q

K
|
K

G

G

E

E

T

S

L

L

F

A

D

D

T

S

A

V

A

Q

N

T

L

M

D

S

A

C

M

Y

G

A

P

-

D

D

A

V

I

V

V

L

R
|
R

H

H

N

P

R

Q

S

P

G

G

V

A

P

V

K

E

I

L

L

A

S

A

K

K

Y

H

V

C

K

R

C

R

S

P

I

V

I

I

N

N

A

A

G

G

D

D

G

G

A

V

H

G

Q

E

H
|
H

P

P

T

T

Q
|
Q

G

A

L

L

D

D

L

I

F

F

T

T

L

I

L

R

Q

E

K

E

F

L

K

G

T

T

L

V

E

N

G

G

K

M

T

T

I

I

A

T

I

M

V

V

G

G

D

D

I

L

R

K

N

H

S

G

R
|
R

V

T

A

V

N

H

S

S

D

L

I

A

E

C

L

L

F

L

T

T

R

Q

F

Y

G

R

A

V

N

S

V

L

I

R

L

Y

V

V

G

A

P

P

Q

S

F

L

-

R

L

M

P

P

K

P

T

T

T

V

L

R

P

A

T

F

T

V

Y

A

-

S

-

R

-

G

-

T

-

K

-

Q

-

E

-

F

-

E

N

S

L

I

K

E

E

E

A

A

I

L

N

P

H

D

S

T

D

D

A

V

I

L

I

Y

S

M

L
x
T

R
|
R

T

I

Q
|
Q

T

K

E

E

R

R

H

-

K

-

T

-

P

-

T

-

F

F

A

G

S

S

L

T

K

Q

D

E

Y

Y

A

E

E

A

-

C

-

F

-

G

N

Q

F

F

K

I

I

L

T

T

K

P

E

H

V

I

I

M

-

T

-

R

K

A

D

K

K

K

D

K

I

M

L

V

I

V

M

M

H

H

P
|
P

G
x
M

P

P

V

-

H

-

R

R

N

V

I

N

D

E

I

I

D

S

D

V

E

E

V

V

L

D

E

S

D

D

E

P

R

R

C

A

L

A

V

Y

L

F

N

R

Q

Q

V

A

K

E

N

N

G
|
G

V

M

A

Y

V

I

R

R

M

M

G

A

I

L

L

L

active site: R57 (= R50), T58 (= T51), K85 (= K78), R106 (= R100), H134 (= H128), Q137 (= Q131), T227 (≠ L210), P266 (= P249), G292 (= G277)
binding n-(phosphonacetyl)-l-aspartic acid: S55 (= S48), T56 (= T49), R57 (= R50), T58 (= T51), S82 (= S75), K85 (= K78), R106 (= R100), H134 (= H128), R167 (= R161), R228 (= R211), Q230 (= Q213), M267 (≠ G250)

P08955 Multifunctional protein CAD; Carbamoyl phosphate synthetase 2-aspartate transcarbamylase-dihydroorotase; EC 6.3.5.5; EC 3.5.1.2; EC 6.3.4.16; EC 2.1.3.2; EC 3.5.2.3 from Mesocricetus auratus (Golden hamster) (see paper)
31% identity, 98% coverage: 1:285/291 of query aligns to 1922:2218/2225 of P08955

query
sites
P08955

M

V

S

G

K

Q

H

H

L

I

T

L

H

S

T

V

K

K

D

Q

L

F

S

T

K

K

N

D

E

Q

I

M

V

S

T

H

I

L

F

F

K

N

Y

V

A

A

K

H

E

T

F

L

L

R

-

M

-

V

-

Q

D

K

E

E

K

R

K

S

R

L

D

D

D

I

L

L

R

K

G

G

K

K

L

V

I

M

I

A

N

S

I

M

F

F

F

Y

E

E

N

V

S

S

T

T

R

R

T

T

R

S

S

S

F

F

E

A

I

A

A

A

A

M

K

A

R

R

L

L

S

G

A

G

D

A

V

V

V

L

N

S

L

F

D

S

V

E

N

A

R

T

S

S

T

V

A

Q

K

K

G

G

E

E

T

S

L

L

F

A

D

D

T

S

A

V

A

Q

N

T

L

M

D

S

A

C

M

Y

G

A

P

-

D

D

A

V

I

V

V

L

R

R

H

H

N

P

R

Q

S

P

G

G

V

A

P

V

K

E

I

L

L

A

S

A

K

K

Y

H

V

C

K

R

C

R

S

P

I

V

I

I

N

N

A

A

G

G

D

D

G

G

A

V

H

G

Q

E

H

H

P

P

T

T

Q

Q

G

A

L

L

D

D

L

I

F

F

T

T

L

I

L

R

Q

E

K

E

F

L

K

G

T

T

L

V

E

N

G

G

K

M

T

T

I

I

A

T

I

M

V

V

G

G

D

D

I

L

R

K

N

H

S

G

R

R

V

T

A

V

N

H

S

S

D

L

I

A

E

C

L

L

F

L

T

T

R

Q

F

Y

G

R

A

V

N

S

V

L

I

R

L

Y

V

V

G

A

P

P

Q

S

F

L

-

R

L

M

P

P

K

P

-

S

-

V

-

W

-

D

-

F

-

V

-

A

-

S

-

R

-

G

T

T

T

K

L

Q

P

E

T

E

T

F

Y

E

N

S

L

I

K

E

E

E

A

A

I

L

N

P

H

D

S

T

D

D

A

V

I

L

I

Y

S

M

L

T

R

R

T

I

Q

Q

T

K

E

E

R

R

H

-

K

-

T

-

P

-

T

-

F

F

A

G

S

S

L

T

K

Q

D

E

Y

Y

A

E

E

A

-

C

-

F

-

G

N

Q

F

F

K

I

I

L

T

T

K

P

E

H

V

I

I

M

K

T

-

R

-

A

D

K

K

K

D

K

I

M

L

V

I

V

M

M

H

H

P

P

G

M

P

P

V

-

H

-

R

R

N

V

I

N

D

E

I

I

D

S

D

V

E

E

V

V

L

D

E

S

D

D

E

P

R

R

C

A

L

A

V

Y

L

F

N

R

Q

Q

V

A

K

E

N

N

G

G

V

M

A

Y

V

I

R

R

M

M

G

A

I

L

L

L

Sites not aligning to the query:

1406 modified: Phosphoserine; by PKA; S→A: No effect on enzyme kinetics.; S→D: Increases CPSase activity and reduces sensitivity to feedback inhibition by UTP.

P27708 Multifunctional protein CAD; Carbamoyl phosphate synthetase 2-aspartate transcarbamylase-dihydroorotase; EC 6.3.5.5; EC 3.5.1.2; EC 6.3.4.16; EC 2.1.3.2; EC 3.5.2.3 from Homo sapiens (Human) (see 7 papers)
31% identity, 98% coverage: 1:285/291 of query aligns to 1922:2218/2225 of P27708

query
sites
P27708

M
x
V

S
x
G

K
x
Q

H
|
H

L
x
I

T
x
L

H
x
S

T
x
V

K
x
Q

D
x
Q

L
x
F

S
x
T

K
|
K

N
x
D

E
x
Q

I
x
M

V
x
S

T
x
H

I
x
L

F
|
F

K
x
N

Y
x
V

A
|
A

K
x
H

E
x
T

F
x
L

L
x
R

-
x
M

-
x
V

-
x
Q

D
x
K

E
|
E

K
x
R

K
x
S

R
x
L

D
|
D

D
x
I

L
|
L

R
x
K

G
|
G

K
|
K

L
x
V

I
x
M

I
x
A

N
x
S

I
x
M

F
|
F

F
x
Y

E
|
E

N
x
V

S
|
S

T
|
T

R
|
R

T
|
T

R
x
S

S
|
S

F
|
F

E
x
A

I
x
A

A
|
A

A
x
M

K
x
A

R
|
R

L
|
L

S
x
G

A
x
G

D
x
A

V
|
V

V
x
L

N
x
S

L
x
F

D
x
S

V
x
E

N
x
A

R
x
T

S
|
S

T
x
V

A
x
Q

K
|
K

G
|
G

E
|
E

T
x
S

L
|
L

F
x
A

D
|
D

T
x
S

A
x
V

A
x
Q

N
x
T

L
x
M

D
x
S

A
x
C

M
x
Y

G
x
A

P

-

D
|
D

A
x
V

I
x
V

V
x
L

R
|
R

H
|
H

N
x
P

R
x
Q

S
x
P

G
|
G

V
x
A

P
x
V

K
x
E

I
x
L

L
x
A

S
x
A

K
|
K

Y
x
H

V
x
C

K
x
R

C
x
R

S
x
P

I
x
V

I
|
I

N
|
N

A
|
A

G
|
G

D
|
D

G
|
G

A
x
V

H
x
G

Q
x
E

H
|
H

P
|
P

T
|
T

Q
|
Q

G
x
A

L
|
L

D
|
D

L
x
I

F
|
F

T
|
T

L
x
I

L
x
R

Q
x
E

K
x
E

F
x
L

K
x
G

T
|
T

L
x
V

E
x
N

G
|
G

K
x
M

T
|
T

I
|
I

A
x
T

I
x
M

V
|
V

G
|
G

D
|
D

I
x
L

R
x
K

N
x
H

S
x
G

R
|
R

V
x
T

A
x
V

N
x
H

S
|
S

D
x
L

I
x
A

E
x
C

L
|
L

F
x
L

T
|
T

R
x
Q

F
x
Y

G
x
R

A
x
V

N
x
S

V
x
L

I
x
R

L
x
Y

V
|
V

G
x
A

P
|
P

Q
x
S

F
x
L

-
x
R

L
x
M

P
|
P

K
x
P

T
|
T

T
x
V

L
x
R

P
x
A

T
x
F

T
x
V

Y
x
A

-
x
S

-
x
R

-
x
G

-
x
T

-
x
K

-
x
Q

-
x
E

-
x
F

-
x
E

N
x
S

L
x
I

K
x
E

E
|
E

A
|
A

I
x
L

N
x
P

H
x
D

S
x
T

D
|
D

A
x
V

I
x
L

I
x
Y

S
x
M

L
x
T

R
|
R

T
x
I

Q
|
Q

T
x
K

E
|
E

R
|
R

H

-

K

-

T

-

P

-

T

-

F
|
F

A
x
G

S
|
S

L
x
T

K
x
Q

D
x
E

Y
|
Y

A
x
E

E
x
A

-
x
C

-
x
F

-
x
G

N
x
Q

F
|
F

K
x
I

I
x
L

T
|
T

K
x
P

E
x
H

V
x
I

I
x
M

K
x
T

-
x
R

-
x
A

D
x
K

K
|
K

D
x
K

I
x
M

L
x
V

I
x
V

M
|
M

H
|
H

P
|
P

G
x
M

P
|
P

V

-

H

-

R
|
R

N
x
V

I
x
N

D
x
E

I
|
I

D
x
S

D
x
V

E
|
E

V
|
V

L
x
D

E
x
S

D
|
D

E
x
P

R
|
R

C
x
A

L
x
A

V
x
Y

L
x
F

N
x
R

Q
|
Q

V
x
A

K
x
E

N
|
N

G
|
G

V
x
M

A
x
Y

V
x
I

R
|
R

M
|
M

G
x
A

I
x
L

L
|
L

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylalanine
33 M → R: in DEE50; uncertain significance; dbSNP:rs751610198
177 R → Q: in a colorectal cancer sample; somatic mutation; dbSNP:rs374122292
456 modified: Phosphothreonine; by MAPK1
735 Y → C: in a colorectal cancer sample; somatic mutation
1406 modified: Phosphoserine; by PKA
1471 binding Zn(2+); binding Zn(2+); H→A: No zinc-binding and no catalytic activity.; H→N: Abolishes dihydroorotase activity.
1473 binding Zn(2+); H→A: No zinc-binding and no catalytic activity.
1475 binding (S)-dihydroorotate
1505 binding (S)-dihydroorotate
1512 D→N: No change in catalytic activity.
1556 binding via carbamate group; binding via carbamate group; modified: N6-carboxylysine
1562 T→A: Abolishes dihydroorotase activity.
1563 F→A: Abolishes dihydroorotase activity.
1590 binding Zn(2+); H→A: Abolishes dihydroorotase activity.; H→N: No catalytic activity.
1613 binding Zn(2+); C→S: Reduces dihydroorotase activity.
1614 binding Zn(2+); H→A: Abolishes dihydroorotase activity.
1637 binding Zn(2+); E→T: Abolishes dihydroorotase activity.
1642 H→N: 11.5% of wild-type catalytic activity.
1661 binding (S)-dihydroorotate
1686 binding Zn(2+); D→N: Abolishes dihydroorotase activity.
1690 binding (S)-dihydroorotate; H→N: 3% of wild-type catalytic activity.
1702 binding (S)-dihydroorotate
1789:2225 natural variant: Missing (in DEE50; uncertain significance)
1859 modified: Phosphoserine; by RPS6KB1 and PKA
1873 modified: Phosphoserine; by PKC; in vitro; S→A: Abolishes PMA-induced Thr-456 phosphorylation.
1900 modified: Phosphoserine

P07259 Multifunctional protein URA2; Pyrimidine-specific carbamoyl phosphate synthase-aspartate carbamoyl transferase; CPSase-ATCase; EC 6.3.5.5; EC 3.5.1.2; EC 6.3.4.16; EC 2.1.3.2 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
35% identity, 99% coverage: 3:289/291 of query aligns to 1911:2210/2214 of P07259

query
sites
P07259

K

R

H

H

L

I

T

L

H

S

T

I

K

K

D

Q

L

F

S

K

K

R

N

S

E

D

I

F

V

H

T

V

I

L

F

F

K

A

Y

V

A

A

K

Q

E

E

F

L

L

R

D

A

E

A

K

V

K

A

R

R

-

E

-

G

-

V

-

L

D

D

D

L

L

M

R

K

G

G

K

H

L

V

I

I

I

T

N

T

I

I

F

F

E

E

N

P

S

S

T

T

R

R

T

T

R

C

S

S

F

F

E

I

I

A

A

A

A

M

K

E

R

R

L

L

S

G

A

G

D

R

V

I

V

V

N

N

L

V

D

N

V

P

N

L

R

V

S

S

T

V

A

K

K

K

G

G

E

E

T

T

L

L

F

Q

D

D

T

T

A

I

A

R

N

T

L

L

D

-

A

A

M

C

G

Y

P

S

D

D

A

A

I

I

I

V

V

M

R

R

H

H

N

S

R

E

S

E

G

M

V

S

P

V

K

H

I

I

L

A

S

A

K

K

Y

Y

V

S

K

P

C

V

S

P

I

I

N

N

A

G

G

G

D

N

G

G

A

S

H

R

Q

E

H

H

P

P

T

T

Q

Q

G

A

L

F

L

L

D

D

L

L

F

F

T

T

L

I

L

R

Q

E

K

E

F

I

K

G

T

T

L

V

E

N

G

G

K

I

T

T

I

V

A

T

I

F

V

M

G

G

D

D

I

L

R

K

N

H

S

G

R

R

V

T

A

V

N

H

S

S

D

L

I

C

E

R

L

L

F

L

T

M

R

H

F

Y

G

Q

A

V

N

R

V

I

I

N

L

L

V

V

G

S

P

P

Q

E

F

L

-

R

L

L

P

P

-

E

-

G

-

L

-

R

-

E

-

L

-

R

K

K

T

A

T

G

L

L

P

L

T

G

T

V

Y

E

N

S

L

I

K

E

-

L

-

T

-

P

E

H

A

I

I

I

N

S

H

K

S

T

D

D

A

V

I

L

I

Y

S

C

L

T

R

R

T

V

Q

Q

T

E

E

E

R

R

H

F

K

N

T

S

P

P

-

E

T

E

F

Y

A

A

S

R

L

L

K

K

D

D

-

T

Y

Y

A

I

E

V

N

D

F

N

K

K

I

I

T

L

K

A

E

H

V

A

I

-

K

-

D

K

K

E

D

N

I

M

L

A

I

I

M

M

H

H

P

P

G

L

P

P

V

-

H

-

R

R

N

V

I

N

D

E

I

I

D

K

D

E

E

E

V

V

L

D

E

Y

D

D

E

H

R

R

C

A

L

A

V

Y

L

F

N

R

Q

Q

V

M

K

K

N

Y

G

G

V

L

A

F

V

V

R

R

M

M

G

A

I

L

L

L

K

A

F

M

L

V

I

M

Sites not aligning to the query:

1857 modified: Phosphoserine; by PKA

5g1pA Aspartate transcarbamoylase domain of human cad bound to carbamoyl phosphate (see paper)
31% identity, 98% coverage: 1:285/291 of query aligns to 1:285/292 of 5g1pA

query
sites
5g1pA

M

V

S

G

K

Q

H

H

L

I

T

L

H

S

T

V

K

Q

D

Q

L

F

S

T

K

K

N

D

E

Q

I

M

V

S

T

H

I

L

F

F

K

N

Y

V

A

A

K

H

-

T

-

L

E

R

F

M

L

M

D

V

E

Q

K

K

K

E

R

R

-

S

-

L

D

D

D

I

L

L

R

K

G

G

K

K

L

V

I

M

I

A

N

S

I

M

F

F

F

Y

E

E

N

V

S
|
S

T
|
T

R
|
R

T
|
T

R

S

S

S

F

F

E

A

I

A

A

A

A

M

K

A

R

R

L

L

S

G

A

G

D

A

V

V

V

L

N

S

L

F

D

S

V

E

N

A

R

T

S

S

T

V

A

Q

K
|
K

G

G

E

E

T

S

L

L

F

A

D

D

T

S

A

V

A

Q

N

T

L

M

D

S

A

C

M

Y

G

A

P

-

D

D

A

V

I

V

V

L

R
|
R

H

H

N

P

R

Q

S

P

G

G

V

A

P

V

K

E

I

L

L

A

S

A

K

K

Y

H

V

C

K

R

C

R

S

P

I

V

I

I

N

N

A

A

G

G

D

D

G

G

A

V

H

G

Q

E

H
|
H

P

P

T

T

Q
|
Q

G

A

L

L

D

D

L

I

F

F

T

T

L

I

L

R

Q

E

K

E

F

L

K

G

T

T

L

V

E

N

G

G

K

M

T

T

I

I

A

T

I

M

V

V

G

G

D

D

I

L

R

K

N

H

S

G

R

R

V

T

A

V

N

H

S

S

D

L

I

A

E

C

L

L

F

L

T

T

R

Q

F

Y

G

R

A

V

N

S

V

L

I

R

L

Y

V

V

G

A

P

P

Q

S

F

L

-

R

L

M

P

P

K

P

T

T

T

V

L

R

P

A

T

F

T

V

Y

A

-

S

-

G

-

T

-

K

-

Q

-

E

-

F

-

E

N

S

L

I

K

E

E

E

A

A

I

L

N

P

H

D

S

T

D

D

A

V

I

L

I

Y

S

M

L
x
T

R

R

T

I

Q

Q

T

K

E

E

R

R

H

F

K

Q

T

-

P

-

T

-

F

-

A

-

S

-

L

-

K

-

D

-

Y

-

A

-

E

-

N

-

F

F

K

I

I

L

T

T

K

P

E

H

V

I

I

M

-

T

-

R

K

A

D

K

K

K

D

K

I

M

L

V

I

V

M

M

H

H

P
|
P

G
x
M

P

P

V

-

H

-

R

R

N

V

I

N

D

E

I

I

D

S

D

V

E

E

V

V

L

D

E

S

D

D

E

P

R

R

C

A

L

A

V

Y

L

F

N

R

Q

Q

V

A

K

E

N

N

G
|
G

V

M

A

Y

V

I

R

R

M

M

G

A

I

L

L

L

active site: R54 (= R50), T55 (= T51), K82 (= K78), R103 (= R100), H131 (= H128), Q134 (= Q131), T223 (≠ L210), P251 (= P249), G277 (= G277)
binding phosphoric acid mono(formamide)ester: S52 (= S48), T53 (= T49), R54 (= R50), T55 (= T51), R103 (= R100), Q134 (= Q131), M252 (≠ G250)

P20054 Multifunctional protein pyr1-3; EC 6.3.5.5; EC 3.5.1.2; EC 6.3.4.16; EC 2.1.3.2; EC 3.5.2.3 from Dictyostelium discoideum (Social amoeba)
32% identity, 99% coverage: 2:288/291 of query aligns to 1922:2220/2225 of P20054

query
sites
P20054

S

G

K

K

H

H

L

I

T

F

H

S

T

V

K

K

D

Q

L

F

S

N

K

R

N

K

E

Q

I

L

V

H

T

A

I

L

F

F

K

G

Y

I

A

A

K

H

E

E

F

M

L

R

D

I

E

L

K

V

K

K

R

R

-

S

-

G

-

S

D

D

D

L

L

L

R

K

G

G

K

K

L

V

I

L

I

A

N

T

I

L

F

F

F

Y

E

E

N

P

S

S

T

T

R

R

T

T

R

Q

S

C

S

S

F

F

E

T

I

A

A

A

A

M

K

Q

R

R

L

L

S

G

A

G

D

S

V

V

N

T

L

V

D

D

V

N

N

V

R

S

S

S

T

V

A

A

K

K

G

G

E

E

T

S

L

I

F

A

D

D

T

T

A

I

A

Q

N

T

L

L

D

E

A

S

M

Y

G

C

P

-

D

D

A

A

I

V

I

C

V

M

R

R

H

H

N

P

R

A

S

V

G

G

V

S

P

V

K

E

I

S

L

A

S

I

K

Q

Y

V

V

A

K

K

C

K

S

P

I

I

N

N

A

A

G

G

D

D

G

G

A

V

H

G

Q

E

H

H

P

P

T

T

Q

Q

G

A

L

L

D

D

L

V

F

F

T

T

L

I

L

R

Q

E

K

E

F

L

K

G

T

T

L

V

E

N

G

G

K

L

T

T

I

I

A

T

I

V

V

V

G

G

D

D

I

L

R

K

N

H

S

G

R

R

V

T

A

V

N

H

S

S

D

L

I

V

E

R

L

L

F

L

T

A

R

N

F

Y

G

Q

A

V

N

K

V

I

I

N

L

Y

V

V

G

S

P

P

P

S

Q

S

F

L

L

-

P

-

K

-

T

-

T

S

L

M

P

P

T

T

T

E

Y

I

-

I

-

K

-

E

-

L

-

N

-

E

-

K

-

G

-

I

-

E

-

Q

-

K

-

E

-

Y

-

T

N

N

L

I

K

E

E

S

A

I

I

L

N

P

H

T

S

T

D

N

A

V

I

L

I

Y

S

V