SitesBLAST
Comparing WP_007473501.1 NCBI__GCF_000170735.1:WP_007473501.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
P56081 3-dehydroquinate synthase; DHQS; EC 4.2.3.4 from Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) (see paper)
52% identity, 98% coverage: 5:339/341 of query aligns to 6:342/343 of P56081
3clhA Crystal structure of 3-dehydroquinate synthase (dhqs)from helicobacter pylori (see paper)
47% identity, 98% coverage: 5:339/341 of query aligns to 3:307/308 of 3clhA
- active site: R107 (= R109), K129 (= K131), E171 (= E173), K207 (= K207), R212 (= R221), N216 (= N225), H219 (= H228), H223 (= H232), H236 (= H245)
- binding nicotinamide-adenine-dinucleotide: I33 (≠ K35), V34 (≠ I36), H38 (= H40), S58 (≠ D60), E60 (= E62), K63 (= K65), G91 (= G93), G92 (= G94), V93 (= V95), D96 (= D98), T116 (= T118), T117 (= T119), L119 (= L121), D123 (= D125), A124 (≠ S126), K129 (= K131), N139 (= N141), T159 (= T161), L160 (= L162), E164 (= E166)
Q9KNV2 3-dehydroquinate synthase; DHQS; EC 4.2.3.4 from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
41% identity, 99% coverage: 2:337/341 of query aligns to 3:356/361 of Q9KNV2
Q5NFS1 3-dehydroquinate synthase; DHQS; EC 4.2.3.4 from Francisella tularensis subsp. tularensis (strain SCHU S4 / Schu 4)
46% identity, 93% coverage: 7:323/341 of query aligns to 10:343/359 of Q5NFS1
3okfA 2.5 angstrom resolution crystal structure of 3-dehydroquinate synthase (arob) from vibrio cholerae
41% identity, 99% coverage: 2:337/341 of query aligns to 4:355/360 of 3okfA
- active site: R120 (= R109), K142 (= K131), E184 (= E173), K226 (= K207), R238 (= R221), N242 (= N225), H245 (= H228), H249 (= H232), H262 (= H245)
- binding nicotinamide-adenine-dinucleotide: N42 (= N33), L48 (= L39), D71 (= D60), E73 (= E62), K76 (= K65), G104 (= G93), G105 (= G94), V106 (= V95), D109 (= D98), T129 (= T118), T130 (= T119), L132 (= L121), D136 (= D125), T172 (= T161), L173 (= L162), E177 (= E166)
U3KRF2 3-dehydroquinate synthase, chloroplastic; EC 4.2.3.4 from Actinidia chinensis var. chinensis (Chinese soft-hair kiwi) (see paper)
43% identity, 93% coverage: 26:341/341 of query aligns to 115:444/445 of U3KRF2
- N122 (= N33) binding NAD(+)
- DGE 153:155 (= DGE 60:62) binding NAD(+)
- K158 (= K65) binding NAD(+)
- GGVIGD 186:191 (≠ GGVVGD 93:98) binding NAD(+)
- TT 211:212 (= TT 118:119) binding NAD(+)
- K224 (= K131) binding NAD(+)
- K233 (= K140) binding NAD(+)
- TLNT 251:254 (≠ FLST 158:161) binding NAD(+)
- K308 (= K207) binding NAD(+)
3zokA Structure of 3-dehydroquinate synthase from actinidia chinensis in complex with NAD (see paper)
43% identity, 95% coverage: 17:341/341 of query aligns to 26:364/365 of 3zokA
- active site: R122 (= R109), K144 (= K131), E186 (= E173), K228 (= K207), E238 (= E217), R242 (= R221), N246 (= N225), H249 (= H228), H253 (= H232), H266 (= H245)
- binding glycine: K144 (= K131), K228 (= K207), R242 (= R221)
- binding nicotinamide-adenine-dinucleotide: T44 (≠ K35), V45 (≠ I36), D73 (= D60), E75 (= E62), K78 (= K65), G106 (= G93), G107 (= G94), V108 (= V95), D111 (= D98), T131 (= T118), T132 (= T119), M134 (≠ L121), D138 (= D125), S139 (= S126), K144 (= K131), K153 (= K140), T174 (= T161), L175 (= L162), E179 (= E166), H266 (= H245)
5eksA Structure of 3-dehydroquinate synthase from acinetobacter baumannii in complex with NAD
42% identity, 90% coverage: 26:332/341 of query aligns to 35:345/355 of 5eksA
- active site: R120 (= R109), K142 (= K131), E184 (= E173), K226 (= K207), R237 (= R221), N241 (= N225), H244 (= H228), H248 (= H232), H261 (= H245)
- binding magnesium ion: E184 (= E173), H244 (= H228), H261 (= H245)
- binding nicotinamide-adenine-dinucleotide: N42 (= N33), V45 (≠ I36), D71 (= D60), E73 (= E62), K76 (= K65), G104 (= G93), G105 (= G94), V106 (= V95), D109 (= D98), T129 (= T118), T130 (= T119), D136 (= D125), S137 (= S126), K142 (= K131), T172 (= T161), L173 (= L162), E177 (= E166)
5hvnA 3.0 angstrom crystal structure of 3-dehydroquinate synthase (arob) from francisella tularensis in complex with NAD.
44% identity, 93% coverage: 7:323/341 of query aligns to 13:338/354 of 5hvnA
- active site: R123 (= R109), K145 (= K131), E187 (= E173), K228 (= K207), R239 (= R221), N243 (= N225), H246 (= H228), H250 (= H232), H263 (= H245)
- binding nicotinamide-adenine-dinucleotide: N45 (= N33), L51 (= L39), D73 (= D60), E75 (= E62), K78 (= K65), G107 (= G93), G108 (= G94), V109 (= V95), D112 (= D98), T132 (= T118), T133 (= T119), L135 (= L121), D139 (= D125), K145 (= K131), F172 (= F158), T175 (= T161), L176 (= L162), E180 (= E166)
6llaB Crystal structure of providencia alcalifaciens 3-dehydroquinate synthase (dhqs) in complex with mg2+ and NAD (see paper)
40% identity, 97% coverage: 2:332/341 of query aligns to 4:354/363 of 6llaB
- active site: R121 (= R109), K143 (= K131), E185 (= E173), K227 (= K207), E237 (= E217), R242 (= R221), N246 (= N225), H249 (= H228), H253 (= H232), H266 (= H245)
- binding magnesium ion: E185 (= E173), H249 (= H228), H266 (= H245)
- binding nicotinamide-adenine-dinucleotide: L46 (≠ I36), D72 (= D60), E74 (= E62), K77 (= K65), G105 (= G93), G106 (= G94), V107 (= V95), D110 (= D98), T130 (= T118), T131 (= T119), L133 (= L121), D137 (= D125), K143 (= K131), T173 (= T161), L174 (= L162), E178 (= E166)
6lk2A Crystal structure of providencia alcalifaciens 3-dehydroquinate synthase (dhqs) in complex with mg2+, NAD and chlorogenic acid (see paper)
39% identity, 97% coverage: 2:332/341 of query aligns to 4:350/357 of 6lk2A
- active site: R121 (= R109), K143 (= K131), E185 (= E173), K227 (= K207), R238 (= R221), N242 (= N225), H245 (= H228), H249 (= H232), H262 (= H245)
- binding (1R,3R,4S,5R)-3-[3-[3,4-bis(oxidanyl)phenyl]propanoyloxy]-1,4,5-tris(oxidanyl)cyclohexane-1-carboxylic acid: D137 (= D125), E185 (= E173), K227 (= K207), R238 (= R221), N242 (= N225), H245 (= H228), T246 (= T229), H249 (= H232), H262 (= H245)
- binding magnesium ion: E185 (= E173), H245 (= H228), H262 (= H245)
- binding nicotinamide-adenine-dinucleotide: L46 (≠ I36), D72 (= D60), E74 (= E62), K77 (= K65), G105 (= G93), G106 (= G94), V107 (= V95), D110 (= D98), T130 (= T118), T131 (= T119), L133 (= L121), D137 (= D125), S138 (= S126), C170 (≠ F158), T173 (= T161), L174 (= L162), P175 (= P163), E178 (= E166)
P9WPX9 3-dehydroquinate synthase; DHQS; EC 4.2.3.4 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
37% identity, 84% coverage: 33:320/341 of query aligns to 37:340/362 of P9WPX9
1dqsA Crystal structure of dehydroquinate synthase (dhqs) complexed with carbaphosphonate, NAD+ and zn2+ (see paper)
40% identity, 79% coverage: 50:319/341 of query aligns to 66:362/381 of 1dqsA
- active site: R127 (= R109), K149 (= K131), E191 (= E173), K240 (= K207), E250 (= E217), R254 (= R221), N258 (= N225), H261 (= H228), H265 (= H232), H277 (= H245)
- binding [1r-(1alpha,3beta,4alpha,5beta)]-5-(phosphonomethyl)-1,3,4-trihydroxycyclohexane-1-carboxylic acid: D143 (= D125), K149 (= K131), N159 (= N141), E191 (= E173), K240 (= K207), R254 (= R221), L257 (= L224), N258 (= N225), H261 (= H228), H265 (= H232), H277 (= H245), K346 (= K303)
- binding nicotinamide-adenine-dinucleotide: E78 (= E62), K81 (= K65), G111 (= G93), G112 (= G94), V113 (= V95), D116 (= D98), T136 (= T118), T137 (= T119), L139 (= L121), D143 (= D125), S144 (= S126), K158 (= K140), T179 (= T161), P181 (= P163), E184 (= E166), H277 (= H245)
- binding zinc ion: E191 (= E173), H261 (= H228), H277 (= H245)
Sites not aligning to the query:
3qbeA Crystal structure of the 3-dehydroquinate synthase (arob) from mycobacterium tuberculosis
36% identity, 84% coverage: 33:320/341 of query aligns to 32:331/352 of 3qbeA
- active site: R117 (= R109), K139 (= K131), E181 (= E173), K223 (= K207), R233 (= R221), N237 (= N225), H240 (= H228), H244 (= H232), H256 (= H245)
- binding zinc ion: E181 (= E173), H240 (= H228), H256 (= H245)
1nvbB Crystal structure of 3-dehydroquinate synthase (dhqs) in complex with zn2+ and carbaphosphonate (see paper)
39% identity, 79% coverage: 50:319/341 of query aligns to 67:370/391 of 1nvbB
- active site: R128 (= R109), K150 (= K131), E192 (= E173), K248 (= K207), E258 (= E217), R262 (= R221), N266 (= N225), H269 (= H228), H273 (= H232), H285 (= H245)
- binding [1r-(1alpha,3beta,4alpha,5beta)]-5-(phosphonomethyl)-1,3,4-trihydroxycyclohexane-1-carboxylic acid: D144 (= D125), K150 (= K131), N160 (= N141), E192 (= E173), K248 (= K207), R262 (= R221), L265 (= L224), N266 (= N225), H269 (= H228), H273 (= H232), K354 (= K303)
- binding zinc ion: E192 (= E173), H269 (= H228), H285 (= H245)
6hqvA Pentafunctional arom complex from chaetomium thermophilum (see paper)
37% identity, 80% coverage: 49:320/341 of query aligns to 61:366/1555 of 6hqvA
- active site: R123 (= R109), K145 (= K131), E187 (= E173), K243 (= K207), E253 (= E217), R257 (= R221), N261 (= N225), H264 (= H228), H268 (= H232), H280 (= H245)
- binding glutamic acid: D139 (= D125), K145 (= K131), E187 (= E173), K243 (= K207), R257 (= R221), H264 (= H228), H280 (= H245)
- binding nicotinamide-adenine-dinucleotide: E76 (= E62), K79 (= K65), G107 (= G93), G108 (= G94), V109 (= V95), D112 (= D98), T132 (= T118), T133 (= T119), L135 (= L121), D139 (= D125), S140 (= S126), K145 (= K131), K154 (= K140), T175 (= T161), L176 (= L162), P177 (= P163), E180 (= E166), H280 (= H245)
- binding zinc ion: E187 (= E173), H264 (= H228), H280 (= H245)
Sites not aligning to the query:
- binding (4S,5R)-4,5-dihydroxy-3-oxocyclohex-1-ene-1-carboxylic acid: 1060, 1062, 1181, 1224, 1232, 1242, 1243
- binding nicotinamide-adenine-dinucleotide: 42, 44, 45
- binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: 413, 562, 563, 874, 923, 924, 979, 1277, 1279, 1323, 1327, 1348, 1368, 1526
P07547 Pentafunctional AROM polypeptide; EC 4.2.3.4; EC 2.5.1.19; EC 2.7.1.71; EC 4.2.1.10; EC 1.1.1.25 from Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) (see 2 papers)
39% identity, 78% coverage: 53:319/341 of query aligns to 72:372/1583 of P07547
- EVSK 81:84 (≠ EEYK 62:65) binding NAD(+)
- GGV 114:116 (= GGV 93:95) binding NAD(+)
- D119 (= D98) binding NAD(+)
- TT 139:140 (= TT 118:119) binding NAD(+)
- K161 (= K140) binding NAD(+)
- FLET 179:182 (≠ FLST 158:161) binding NAD(+)
- N190 (≠ A169) binding NAD(+)
- E194 (= E173) binding Zn(2+)
- H271 (= H228) binding Zn(2+)
- H287 (= H245) binding Zn(2+)
Sites not aligning to the query:
6c5cA Crystal structure of the 3-dehydroquinate synthase (dhqs) domain of aro1 from candida albicans sc5314 in complex with nadh (see paper)
39% identity, 81% coverage: 46:320/341 of query aligns to 63:366/385 of 6c5cA
- active site: R130 (= R109), K152 (= K131), E194 (= E173), K246 (= K207), E254 (= E217), R258 (= R221), N262 (= N225), H265 (= H228), H269 (= H232), H281 (= H245)
- binding nicotinamide-adenine-dinucleotide: E83 (= E62), K86 (= K65), G114 (= G93), G115 (= G94), V116 (= V95), D119 (= D98), T139 (= T118), T140 (= T119), D146 (= D125), S147 (= S126), F179 (= F158), T182 (= T161), L183 (= L162), Q187 (≠ E166)
Sites not aligning to the query:
1nvaA Crystal structure of 3-dehydroquinate synthase (dhqs) in complex with zn2+ and adp (see paper)
39% identity, 74% coverage: 50:303/341 of query aligns to 66:340/380 of 1nvaA
- active site: R127 (= R109), K149 (= K131), E191 (= E173), K247 (≠ E217), R257 (= R221), N261 (= N225), H264 (= H228), H268 (= H232), H280 (= H245)
- binding adenosine-5'-diphosphate: G111 (= G93), G112 (= G94), T136 (= T118), T137 (= T119), F176 (= F158), T179 (= T161), L180 (= L162)
- binding zinc ion: E191 (= E173), H264 (= H228), H280 (= H245)
Sites not aligning to the query:
3qbdA 3-dehydroquinate synthase (arob) from mycobacterium tuberculosis in complex with NAD
37% identity, 77% coverage: 33:295/341 of query aligns to 32:305/344 of 3qbdA
- active site: R117 (= R109), K139 (= K131), E181 (= E173), K223 (= K207), R232 (= R221), N236 (= N225), H239 (= H228), H243 (= H232), H255 (= H245)
- binding nicotinamide-adenine-dinucleotide: Q40 (≠ I41), D68 (= D60), A69 (≠ G61), E70 (= E62), K73 (= K65), G101 (= G93), G102 (= G94), A103 (≠ V95), D106 (= D98), T126 (= T118), T127 (= T119), L129 (= L121), A134 (≠ S126), T169 (= T161), L170 (= L162)
Query Sequence
>WP_007473501.1 NCBI__GCF_000170735.1:WP_007473501.1
MKTTIKTPSKTYNIIIDKLPKLNFDRKVAVITNHKIAGLHINYLTNNLSAKELHIITLPD
GEEYKNWQNIEFILDRLFDAKFDRNSLLIAFGGGVVGDMTGFAASIFLRGVDFIQIPTTL
LAMVDSSVGGKTGINNKYGKNLIGSFYQPNAVYIDTHFLSTLPKREFAAGTAEIIKMAVM
FDKDFFEKIKNNSISLEKMIKRAVELKAEVVNQDEKEKGIRSVLNYGHTFGHVIENLTNY
KTFLHGEAVAIGMVMANELSKTLGYLSENEAEEIKQVLKKNNLPIDFKIENIEEFYNHFF
LDKKTSNNKIKFIIPEKIGKYKIIDDIDKNIILKVLKKFEK
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory