SitesBLAST

Q58989 Phosphoserine phosphatase; PSP; PSPase; O-phosphoserine phosphohydrolase; EC 3.1.3.3 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) (see 3 papers)
50% identity, 100% coverage: 2:206/206 of query aligns to 6:210/211 of Q58989

query
sites
Q58989

K

K

L

L

A

I

V

L

F

F

D
|
D

F

F

D
|
D

S

S

T

T

L

L

M

V

D

N

G

N

E
|
E

T

T

I

I

D

D

F

E

L

I

A

A

E

R

P

E

L

A

G

G

F

V

K

E

D

E

K

E

V

V

A

K

S

K

I

I

T

T

E

K

M

E

A

A

M

M

R

E

G

G

E

K

L

L

D

N

F

F

F

E

E

Q

S

S

L

L

I

R

M

K

R
|
R

V

V

K

S

L

L

E

K

G

D

L

L

E

P

D

I

K

E

K

K

V

V

N

E

E

K

I

A

C

I

H

K

N

R

L

I

P

T

Y

P

M

T

P

E

G

G

A

A

D

E

E

E

T

T

I

I

K

K

A

E

L

L

K

K

K

N

D

R

G

G

Y

Y

K

V

V

V

V

A

V

V

F

V

S
|
S

G
|
G

G

G

F

F

R

D

N

I

A

A

T

V

S

N

Y

K

A

I

K

K

E

E

I

K

L

L

G

G

F

L

D

D

A

Y

D

A

F

F

S

A

N

N

I

R

L

L

H

I

S

V

K

K

N

D

G

G

R

K

L

L

T

T

G

G

L

D

V

V

G

E

G

G

E

E

M

V

M

L

F

K

S

E

H

N

S

A

K
|
K

G

G

D

E

M

I

L

L

K

E

R

K

L

I

Q

A

A

K

I

I

L

E

G

G

I

I

S

N

I

L

E

E

D

D

T

T

L

V

V

A

V

V

G

G

D
|
D

G

G

A

A

N
|
N

D

D

L

I

S

S

M

M

F

F

K

K

Y

K

A

A

G

G

T

L

R

K

I

I

A

A

F

F

C

C

A

A

K

K

D

P

V

I

L

L

K

K

K

E

E

K

A

A

N

D

V

I

I

C

I

I

E

E

E

K

K

R

D

D

L

L

T

R

K

E

I

I

L

L

E

K

F

Y

I

I

D11 (= D7) active site, Nucleophile; binding Mg(2+); mutation to N: Loss of activity.
D13 (= D9) active site, Proton donor; binding Mg(2+)
E20 (= E16) binding substrate
R56 (= R52) binding substrate
SG 99:100 (= SG 95:96) binding substrate
K144 (= K140) binding substrate
D167 (= D163) binding Mg(2+)
N170 (= N166) binding substrate

1f5sA Crystal structure of phosphoserine phosphatase from methanococcus jannaschii (see paper)
50% identity, 100% coverage: 2:206/206 of query aligns to 5:209/210 of 1f5sA

query
sites
1f5sA

K

K

L

L

A

I

V

L

F

F

D
|
D

F
|
F

D
|
D

S

S

T

T

L

L

M

V

D

N

G

N

E

E

T

T

I

I

D

D

F

E

L

I

A

A

E

R

P

E

L

A

G

G

F

V

K

E

D

E

K

E

V

V

A

K

S

K

I

I

T

T

E

K

M

E

A

A

M

M

R

E

G

G

E

K

L

L

D

N

F

F

F

E

E

Q

S

S

L

L

I

R

M

K

R

R

V

V

K

S

L

L

E

K

G

D

L

L

E

P

D

I

K

E

K

K

V

V

N

E

E

K

I

A

C

I

H

K

N

R

L

I

P

T

Y

P

M

T

P

E

G

G

A

A

D

E

E

E

T

T

I

I

K

K

A

E

L

L

K

K

K

N

D

R

G

G

Y

Y

K

V

V

V

V

A

V

V

F

V

S
|
S

G
|
G

G

G

F

F

R

D

N

I

A

A

T

V

S

N

Y

K

A

I

K

K

E

E

I

K

L

L

G

G

F

L

D

D

A

Y

D

A

F

F

S

A

N

N

I

R

L

L

H

I

S

V

K

K

N

D

G

G

R

K

L

L

T

T

G

G

L

D

V

V

G

E

G

G

E

E

M

V

M

L

F

K

S

E

H

N

S

A

K
|
K

G

G

D

E

M

I

L

L

K

E

R

K

L

I

Q

A

A

K

I

I

L

E

G

G

I

I

S

N

I

L

E

E

D

D

T

T

L

V

V

A

V

V

G

G

D
|
D

G

G

A

A

N

N

D
|
D

L

I

S

S

M

M

F

F

K

K

Y

K

A

A

G

G

T

L

R

K

I

I

A

A

F

F

C

C

A

A

K

K

D

P

V

I

L

L

K

K

K

E

E

K

A

A

N

D

V

I

I

C

I

I

E

E

E

K

K

R

D

D

L

L

T

R

K

E

I

I

L

L

E

K

F

Y

I

I

active site: D10 (= D7), F11 (= F8), D12 (= D9), G99 (= G96), K143 (= K140), D170 (= D167)
binding magnesium ion: D10 (= D7), D12 (= D9), D166 (= D163)
binding phosphate ion: D10 (= D7), F11 (= F8), D12 (= D9), S98 (= S95), G99 (= G96), K143 (= K140)

1l7nA Transition state analogue of phosphoserine phosphatase (aluminum fluoride complex) (see paper)
50% identity, 100% coverage: 2:206/206 of query aligns to 4:208/209 of 1l7nA

query
sites
1l7nA

K

K

L

L

A

I

V

L

F

F

D
|
D

F
|
F

D
|
D

S

S

T

T

L

L

M

V

D

N

G

N

E

E

T

T

I

I

D

D

F

E

L

I

A

A

E

R

P

E

L

A

G

G

F

V

K

E

D

E

K

E

V

V

A

K

S

K

I

I

T

T

E

K

M

E

A

A

M

M

R

E

G

G

E

K

L

L

D

N

F

F

F

E

E

Q

S

S

L

L

I

R

M

K

R

R

V

V

K

S

L

L

E

K

G

D

L

L

E

P

D

I

K

E

K

K

V

V

N

E

E

K

I

A

C

I

H

K

N

R

L

I

P

T

Y

P

M

T

P

E

G

G

A

A

D

E

E

E

T

T

I

I

K

K

A

E

L

L

K

K

K

N

D

R

G

G

Y

Y

K

V

V

V

V

A

V

V

F

V

S
|
S

G
|
G

G

G

F

F

R

D

N

I

A

A

T

V

S

N

Y

K

A

I

K

K

E

E

I

K

L

L

G

G

F

L

D

D

A

Y

D

A

F

F

S

A

N

N

I

R

L

L

H

I

S

V

K

K

N

D

G

G

R

K

L

L

T

T

G

G

L

D

V

V

G

E

G

G

E

E

M

V

M

L

F

K

S

E

H

N

S

A

K
|
K

G

G

D

E

M

I

L

L

K

E

R

K

L

I

Q

A

A

K

I

I

L

E

G

G

I

I

S

N

I

L

E

E

D

D

T

T

L

V

V

A

V

V

G

G

D
|
D

G

G

A

A

N
|
N

D
|
D

L

I

S

S

M

M

F

F

K

K

Y

K

A

A

G

G

T

L

R

K

I

I

A

A

F

F

C

C

A

A

K

K

D

P

V

I

L

L

K

K

K

E

E

K

A

A

N

D

V

I

I

C

I

I

E

E

E

K

K

R

D

D

L

L

T

R

K

E

I

I

L

L

E

K

F

Y

I

I

active site: D9 (= D7), F10 (= F8), D11 (= D9), G98 (= G96), K142 (= K140), D169 (= D167)
binding aluminum fluoride: D9 (= D7), F10 (= F8), D11 (= D9), S97 (= S95), K142 (= K140)
binding tetrafluoroaluminate ion: D9 (= D7), F10 (= F8), D11 (= D9), S97 (= S95), G98 (= G96), K142 (= K140), N168 (= N166)
binding magnesium ion: D9 (= D7), D11 (= D9), D165 (= D163)

1l7pA Substrate bound phosphoserine phosphatase complex structure (see paper)
50% identity, 100% coverage: 2:206/206 of query aligns to 3:207/208 of 1l7pA

query
sites
1l7pA

K

K

L

L

A

I

V

L

F

F

D
x
N

F
|
F

D
|
D

S

S

T

T

L

L

M

V

D

N

G

N

E
|
E

T

T

I

I

D

D

F

E

L

I

A

A

E

R

P

E

L

A

G

G

F

V

K

E

D

E

K

E

V

V

A

K

S

K

I

I

T

T

E

K

M

E

A

A

M
|
M

R

E

G

G

E

K

L

L

D

N

F
|
F

F

E

E

Q

S

S

L

L

I

R

M

K

R
|
R

V

V

K

S

L

L

E

K

G

D

L

L

E

P

D

I

K

E

K

K

V

V

N

E

E

K

I

A

C

I

H

K

N

R

L

I

P

T

Y

P

M

T

P

E

G

G

A

A

D

E

E

E

T

T

I

I

K

K

A

E

L

L

K

K

K

N

D

R

G

G

Y

Y

K

V

V

V

V

A

V

V

F

V

S
|
S

G
|
G

G

G

F

F

R

D

N

I

A

A

T

V

S

N

Y

K

A

I

K

K

E

E

I

K

L

L

G

G

F

L

D

D

A

Y

D

A

F

F

S

A

N

N

I

R

L

L

H

I

S

V

K

K

N

D

G

G

R

K

L

L

T

T

G

G

L

D

V

V

G

E

G

G

E

E

M

V

M

L

F

K

S

E

H

N

S

A

K
|
K

G

G

D

E

M

I

L

L

K

E

R

K

L

I

Q

A

A

K

I

I

L

E

G

G

I

I

S

N

I

L

E

E

D

D

T

T

L

V

V

A

V

V

G

G

D

D

G

G

A

A

N

N

D
|
D

L

I

S

S

M

M

F

F

K

K

Y

K

A

A

G

G

T

L

R

K

I

I

A

A

F

F

C

C

A

A

K

K

D

P

V

I

L

L

K

K

K

E

E

K

A

A

N

D

V

I

I

C

I

I

E

E

E

K

K

R

D

D

L

L

T

R

K

E

I

I

L

L

E

K

F

Y

I

I

active site: N8 (≠ D7), F9 (= F8), D10 (= D9), G97 (= G96), K141 (= K140), D168 (= D167)
binding phosphoserine: N8 (≠ D7), F9 (= F8), D10 (= D9), E17 (= E16), M40 (= M39), F46 (= F45), R53 (= R52), S96 (= S95), G97 (= G96), K141 (= K140)

1l7oA Crystal structure of phosphoserine phosphatase in apo form (see paper)
48% identity, 100% coverage: 2:206/206 of query aligns to 3:199/200 of 1l7oA

query
sites
1l7oA

K

K

L

L

A

I

V

L

F

F

D

N

F

F

D

D

S

S

T

T

L

L

M

V

D

N

G

N

E

E

T

T

I

I

D

D

F

E

L

I

A

A

E

R

P

E

L

A

G

G

F

V

K

E

D

E

K

E

V

V

A

K

S

K

I

I

T

T

E

-

M

-

A

-

M

-

R

-

G

-

E

-

L

-

D

N

F

F

F

E

E

Q

S

S

L

L

I

R

M

K

R

R

V

V

K

S

L

L

E

K

G

D

L

L

E

P

D

I

K

E

K

K

V

V

N

E

E

K

I

A

C

I

H

K

N

R

L

I

P

T

Y

P

M

T

P

E

G

G

A

A

D

E

E

E

T

T

I

I

K

K

A

E

L

L

K

K

K

N

D

R

G

G

Y

Y

K

V

V

V

V

A

V

V

F

V

S

S

G

G

F

F

R

D

N

I

A

A

T

V

S

N

Y

K

A

I

K

K

E

E

I

K

L

L

G

G

F

L

D

D

A

Y

D

A

F

F

S

A

N

N

I

R

L

L

H

I

S

V

K

K

N

D

G

G

R

K

L

L

T

T

G

G

L

D

V

V

G

E

G

G

E

E

M

V

M

L

F

K

S

E

H

N

S

A

K

K

G

G

D

E

M

I

L

L

K

E

R

K

L

I

Q

A

A

K

I

I

L

E

G

G

I

I

S

N

I

L

E

E

D

D

T

T

L

V

V

A

V

V

G

G

D

D

G

G

A

A

N

N

D

D

L

I

S

S

M

M

F

F

K

K

Y

K

A

A

G

G

T

L

R

K

I

I

A

A

F

F

C

C

A

A

K

K

D

P

V

I

L

L

K

K

K

E

E

K

A

A

N

D

V

I

I
x
C

I

I

E
|
E

E

K

K

R

D

D

L

L

T

R

K

E

I

I

L

L

E

K

F

Y

I

I

binding zinc ion: C186 (≠ I193), E188 (= E195)

O53289 Phosphoserine phosphatase SerB2; PSP; PSPase; O-phosphoserine phosphohydrolase; Protein-serine/threonine phosphatase; EC 3.1.3.3; EC 3.1.3.16 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
42% identity, 98% coverage: 2:203/206 of query aligns to 180:381/409 of O53289

query
sites
O53289

K

R

L

L

A

I

V

V

F

F

D
|
D

F
x
V

D
|
D

S
|
S

T

T

L

L

M

V

D

Q

G

G

E

E

T

V

I

I

D

E

F

M

L

L

A

A

E

A

P

R

L

A

G

G

F

A

K

Q

D

G

K

Q

V

V

A

A

S

A

I

I

T

T

E

E

M

A

A

A

M

M

R

R

G

G

E

E

L

L

D

D

F

F

F

A

E

E

S

S

L

L

I

Q

M

R

R

R

V

V

K

A

L

T

L

L

E

A

G

G

L

L

E

P

D

A

K

T

K

V

V

I

N

D

E

D

I

V

C

A

H

E

N

Q

L

L

P

E

Y

L

M

M

P

P

G

G

A

A

D

R

E

T

T

T

I

I

K

R

A

T

L

L

K

R

D

L

G

G

Y

F

K

R

V

C

V

G

V

V

F

V

S
|
S

G

G

F

F

R

R

N

R

A

I

T

I

S

E

Y

P

A

L

K

A

E

R

I

E

L

L

G

M

F

L

D

D

A

F

D

V

F

A

S

S

N

N

I

E

L

L

H

E

S

I

K

V

N

D

G

G

R

I

L

L

T

T

G

G

L

R

V

V

G

V

G

G

E

P

M

I

M

V

F

D

S

R

H

P

S

G

K
|
K

G

A

D

K

M

A

L

L

K

R

R

D

L

F

Q

A

A

S

I

Q

L

Y

G

G

I

V

S

P

I

M

E

E

D

Q

T

T

L

V

V

A

V

V

G

G

D
|
D

G

G

A

A

N

N

D
|
D

L

I

S

D

M

M

F

L

K

G

Y

A

A

A

G

G

T

L

R

G

I

I

A

A

F

F

C

N

A

A

K

K

D

P

V

A

L

L

K

R

K

E

E

V

A

A

N

D

V

A

I

S

I

L

E

S

E

H

K

P

D

Y

L

L

T

D

K

T

I

V

L

L

D185 (= D7) mutation to G: Completely abolishes enzymatic activity.; mutation to N: Completely abolishes enzymatic activity.
V186 (≠ F8) mutation to Q: Decreases enzymatic activity by 50%.
D187 (= D9) mutation to N: Decreases enzymatic activity by 15%.
S188 (= S10) mutation to A: No effect on enzymatic activity.
S273 (= S95) mutation to A: Completely abolishes enzymatic activity (PubMed:25521849). Decreases enzymatic activity by 60% (PubMed:25037224).
K318 (= K140) mutation to A: Decreases enzymatic activity by 50%.; mutation to E: Completely abolishes enzymatic activity.
D341 (= D163) mutation to G: Decreases enzymatic activity by 80%.; mutation to N: Decreases enzymatic activity by 85%. Completely abolishes enzymatic activity, does not elicit cytoskeletal rearrangements, and does not suppress IL-8 production after TNF-alpha stimulation; when associated with N-345.
D345 (= D167) mutation to N: Decreases enzymatic activity by 55%. Completely abolishes enzymatic activity, does not elicit cytoskeletal rearrangements, and does not suppress IL-8 production after TNF-alpha stimulation; when associated with N-341.

Sites not aligning to the query:

18 G→A: Does not bind L-serine and correspondingly no oligomeric transitions is observed in the presence of L-serine.
108 G→A: Does not bind L-serine and correspondingly no oligomeric transitions is observed in the presence of L-serine.

8a21A Crystal structure of phosphoserine phosphatase serb from mycobacterium avium in complex with phenylimidazole (see paper)
41% identity, 98% coverage: 2:203/206 of query aligns to 178:379/396 of 8a21A

query
sites
8a21A

K

R

L

L

A

I

V

V

F

F

D
|
D

F

V

D
|
D

S

S

T

T

L

L

M

V

D

Q

G

G

E
|
E

T
x
V

I
|
I

D

E

F

M

L

L

A

A

E

A

P

K

L

A

G

G

F

A

K

E

D

G

K

Q

V

V

A

A

S

A

I

I

T
|
T

E

D

M

A

A

A

M
|
M

R

R

G

G

E

E

L

L

D

D

F
|
F

F

A

E

Q

S

S

L

L

I

Q

M

Q

R
|
R

V

V

K

A

L

T

L

L

E

A

G

G

L

L

E

P

D

A

K

T

K

V

V

I

N

D

E

E

I

V

C

A

H

G

N

Q

L

L

P

E

Y

L

M

M

P

P

G

G

A

A

D

R

E

T

T

T

I

L

K

R

A

T

L

L

K

R

D

L

G

G

Y

Y

K

A

V

C

V

G

V

V

F

V

S

S

G

G

G
|
G

F

F

R

R

N

R

A

I

T

I

S

E

Y

P

A

L

K

A

E

E

I

E

L

L

G

M

F

L

D

D

A

Y

D

V

F

A

S

A

N

N

I

E

L

L

H

E

S

I

K

V

N

D

G

G

R

T

L

L

T

T

G

G

L

R

V

V

G

V

G

G

E

P

M

I

F

D

S

R

H

A

S

G

K

K

G

A

D

T

M

A

L

L

K

R

R

E

L

F

Q

A

A

Q

I

R

L

A

G

G

I

V

S

P

I

M

E

A

D

Q

T

T

L

V

V

A

V

V

G

G

D
|
D

G

G

A

A

N

N

D

D

L

I

S

D

M

M

F

L

K

A

Y

A

A

A

G

G

T

L

R

G

I

I

A

A

F

F

C

N

A

A

K

K

D

P

V

A

L

L

K

R

K

E

E

V

A

A

N

D

V

A

I

S

I

L

E

S

E

H

K

P

D

Y

L

L

T

D

K

T

I

V

L

L

binding magnesium ion: D183 (= D7), D185 (= D9), D339 (= D163)
binding 4-phenyl-1h-imidazole: D185 (= D9), E192 (= E16), V193 (≠ T17), I194 (= I18), T211 (= T35), M215 (= M39), F221 (= F45), R228 (= R52), G273 (= G97)

Sites not aligning to the query:

binding 4-phenyl-1h-imidazole: 13, 18, 37

8a1zA Crystal structure of phosphoserine phosphatase serb from mycobacterium avium in complex with 1-(2,4-dichlorophenyl)-3-hydroxyurea (see paper)
41% identity, 98% coverage: 2:203/206 of query aligns to 178:379/396 of 8a1zA

query
sites
8a1zA

K

R

L

L

A

I

V

V

F

F

D
|
D

F

V

D
|
D

S

S

T

T

L

L

M

V

D

Q

G

G

E
|
E

T

V

I

I

D

E

F

M

L

L

A

A

E

A

P

K

L

A

G

G

F

A

K

E

D

G

K

Q

V

V

A

A

S

A

I

I

T

T

E

D

M

A

A

A

M
|
M

R

R

G

G

E

E

L

L

D

D

F
|
F

F

A

E

Q

S

S

L
|
L

I

Q

M

Q

R
|
R

V

V

K

A

L

T

L

L

E

A

G

G

L

L

E

P

D

A

K

T

K

V

V

I

N

D

E

E

I

V

C

A

H

G

N

Q

L

L

P

E

Y

L

M

M

P

P

G

G

A

A

D

R

E

T

T

T

I

L

K

R

A

T

L

L

K

R

D

L

G

G

Y

Y

K

A

V

C

V

G

V

V

F

V

S

S

G
|
G

G

G

F
|
F

R

R

N

R

A

I

T

I

S

E

Y

P

A

L

K

A

E

E

I

E

L

L

G

M

F

L

D

D

A

Y

D

V

F

A

S

A

N

N

I

E

L

L

H

E

S

I

K

V

N

D

G

G

R

T

L

L

T

T

G

G

L

R

V

V

G

V

G

G

E

P

M

I

F

D

S

R

H

A

S

G

K

K

G

A

D

T

M

A

L

L

K

R

R

E

L

F

Q

A

A

Q

I

R

L

A

G

G

I

V

S

P

I

M

E

A

D

Q

T

T

L

V

V

A

V

V

G

G

D
|
D

G

G

A

A

N

N

D

D

L

I

S

D

M

M

F

L

K

A

Y

A

A

A

G

G

T

L

R

G

I

I

A

A

F

F

C

N

A

A

K

K

D

P

V

A

L

L

K

R

K

E

E

V

A

A

N

D

V

A

I

S

I

L

E

S

E

H

K

P

D

Y

L

L

T

D

K

T

I

V

L

L

binding 1-(2,4-dichlorophenyl)-3-oxidanyl-urea: D185 (= D9), E192 (= E16), M215 (= M39), F221 (= F45), L225 (= L49), R228 (= R52), G272 (= G96), F274 (= F98), D339 (= D163)
binding magnesium ion: D183 (= D7), D185 (= D9), D339 (= D163)

A0QJI1 Phosphoserine phosphatase; PSP; PSPase; O-phosphoserine phosphohydrolase; EC 3.1.3.3 from Mycobacterium avium (strain 104) (see paper)
41% identity, 98% coverage: 2:203/206 of query aligns to 182:383/411 of A0QJI1

query
sites
A0QJI1

K

R

L

L

A

I

V

V

F

F

D
|
D

F

V

D
|
D

S

S

T

T

L

L

M

V

D

Q

G

G

E

E

T

V

I

I

D

E

F

M

L

L

A

A

E

A

P

K

L

A

G

G

F

A

K

E

D

G

K

Q

V

V

A

A

S

A

I

I

T

T

E

D

M

A

A

A

M

M

R

R

G

G

E

E

L

L

D

D

F

F

F

A

E

Q

S

S

L

L

I

Q

M

Q

R

R

V

V

K

A

L

T

L

L

E

A

G

G

L

L

E

P

D

A

K

T

K

V

V

I

N

D

E

E

I

V

C

A

H

G

N

Q

L

L

P

E

Y

L

M

M

P

P

G

G

A

A

D

R

E

T

T

T

I

L

K

R

A

T

L

L

K

R

D

L

G

G

Y

Y

K

A

V

C

V

G

V

V

F

V

S

S

G

G

F

F

R

R

N

R

A

I

T

I

S

E

Y

P

A

L

K

A

E

E

I

E

L

L

G

M

F

L

D

D

A

Y

D

V

F

A

S

A

N

N

I

E

L

L

H

E

S

I

K

V

N

D

G

G

R

T

L

L

T

T

G

G

L

R

V

V

G

V

G

G

E

P

M

I

F

D

S

R

H

A

S

G

K

K

G

A

D

T

M

A

L

L

K

R

R

E

L

F

Q

A

A

Q

I

R

L

A

G

G

I

V

S

P

I

M

E

A

D

Q

T

T

L

V

V

A

V

V

G

G

D
|
D

G

G

A

A

N

N

D

D

L

I

S

D

M

M

F

L

K

A

Y

A

A

A

G

G

T

L

R

G

I

I

A

A

F

F

C

N

A

A

K

K

D

P

V

A

L

L

K

R

K

E

E

V

A

A

N

D

V

A

I

S

I

L

E

S

E

H

K

P

D

Y

L

L

T

D

K

T

I

V

L

L

D187 (= D7) binding Mg(2+)
D189 (= D9) binding Mg(2+)
D343 (= D163) binding Mg(2+)

5jlpA Crystal structure of mycobacterium avium serb2 in complex with serine at act domain
41% identity, 98% coverage: 2:203/206 of query aligns to 178:379/396 of 5jlpA

query
sites
5jlpA

K

R

L

L

A

I

V

V

F

F

D
|
D

F
x
V

D
|
D

S

S

T

T

L

L

M

V

D

Q

G

G

E

E

T

V

I

I

D

E

F

M

L

L

A

A

E

A

P

K

L

A

G

G

F

A

K

E

D

G

K

Q

V

V

A

A

S

A

I

I

T

T

E

D

M

A

A

A

M

M

R

R

G

G

E

E

L

L

D

D

F

F

F

A

E

Q

S

S

L

L

I

Q

M

Q

R

R

V

V

K

A

L

T

L

L

E

A

G

G

L

L

E

P

D

A

K

T

K

V

V

I

N

D

E

E

I

V

C

A

H

G

N

Q

L

L

P

E

Y

L

M

M

P

P

G

G

A

A

D

R

E

T

T

T

I

L

K

R

A

T

L

L

K

R

D

L

G

G

Y

Y

K

A

V

C

V

G

V

V

F

V

S

S

G
|
G

G

G

F

F

R

R

N

R

A

I

T

I

S

E

Y

P

A

L

K

A

E

E

I

E

L

L

G

M

F

L

D

D

A

Y

D

V

F

A

S

A

N

N

I

E

L

L

H

E

S

I

K

V

N

D

G

G

R

T

L

L

T

T

G

G

L

R

V

V

G

V

G

G

E

P

M

I

F

D

S

R

H

A

S

G

K
|
K

G

A

D

T

M

A

L

L

K

R

R

E

L

F

Q

A

A

Q

I

R

L

A

G

G

I

V

S

P

I

M

E

A

D

Q

T

T

L

V

V

A

V

V

G

G

D
|
D

G

G

A

A

N

N

D
|
D

L

I

S

D

M

M

F

L

K

A

Y

A

A

A

G

G

T

L

R

G

I

I

A

A

F

F

C

N

A

A

K

K

D

P

V

A

L

L

K

R

K

E

E

V

A

A

N

D

V

A

I

S

I

L

E

S

E

H

K

P

D

Y

L

L

T

D

K

T

I

V

L

L

active site: D183 (= D7), V184 (≠ F8), D185 (= D9), G272 (= G96), K316 (= K140), D343 (= D167)
binding magnesium ion: D183 (= D7), D339 (= D163)

Sites not aligning to the query:

binding serine: 13, 14

7qplA Crystal structure of phosphoserine phosphatase (serb) from brucella melitensis in complex with phosphate and magnesium
37% identity, 98% coverage: 2:203/206 of query aligns to 81:283/295 of 7qplA

query
sites
7qplA

K

K

L

I

A

L

V

I

F

A

D
|
D

F

M

D
|
D

S

S

T

T

L

M

M

I

D

G

G

Q

E

E

T

C

I

I

D

D

F

E

L

L

A

A

E

E

P

E

L

A

G

G

F

L

K

R

D

D

K

H

V

V

A

A

S

A

I

I

T

T

E

A

M

R

A

A

M

M

R

N

G

G

E

E

L

I

D

A

F

F

F

E

E

P

S

A

L

L

I

R

M

E

R

R

V

V

K

A

L

L

E

K

G

G

L

L

E

P

D

L

K

S

K

V

V

I

N

D

E

K

-

V

I

I

C

S

H

T

N

R

L

I

P

T

Y

L

M

T

P

P

G

G

A

G

D

P

E

Q

T

L

I

V

K

R

A

T

L

M

K

R

K

K

D

H

G

G

Y

A

K

Y

V

T

V

A

V

L

F

V

S

S

G

G

F

F

R

T

N

S

A

F

T

T

S

R

Y

R

A

I

K

A

E

E

I

M

L

I

G

G

F

F

D

N

A

E

D

E

F

R

S

A

N

N

I

R

L

L

H

I

S

D

K

D

N

G

G

T

R

R

L

L

T

T

G

G

L

T

V

V

G

A

G

E

E

P

M

I

M

L

F

G

S

R

H

E

S

A

K

K

G

V

D

E

M

K

L

L

K

V

R

E

L

I

Q

A

A

E

I

R

L

V

G

G

I

L

S

T

I

P

E

E

D

D

T

A

L

I

V

A

V

V

G

G

D
|
D

G

G

A

A

N

N

D

D

L

L

S

G

M

M

F

I

K

Q

Y

L

A

A

G

G

T

T

R

G

I

V

A

A

F

L

C

H

A

A

K

K

D

P

V

A

L

V

K

A

E

Q

A

A

N

K

V

M

I

R

I

I

E

D

E

H

K

G

D

D

L

L

T

T

K

A

I

L

L

L

binding magnesium ion: D86 (= D7), D88 (= D9), D243 (= D163)

6hyyA Human phosphoserine phosphatase with serine and phosphate (see paper)
29% identity, 85% coverage: 6:180/206 of query aligns to 15:192/221 of 6hyyA

query
sites
6hyyA

F

F

D
|
D

F
x
V

D
|
D

S

S

T

T

L

V

M

I

D

R

G

E

E

E

T

G

I

I

D

D

F

E

L

L

A

A

E

K

P

I

L

C

G

G

F

V

K

E

D

D

K

A

V

V

A

S

S

E

I

M

T

T

E

R

M

R

A

A

M

M

R

G

G

G

E

A

L

V

D

P

F

F

F

K

E

A

S

A

L

L

I

T

M

E

R

R

V

L

K

A

L

L

L

I

E

Q

G

P

L

S

E

R

D

E

K

Q

K

V

V

Q

N

R

E

L

I

I

C

A

H

E

N

Q

L

P

P

P

Y

H

M

L

-

T

P

P

G

G

A

I

D

R

E

E

T

L

I

V

K

S

A

R

L

L

K

Q

K

E

D

R

G

N

Y

V

K

Q

V

V

V

F

V

L

F

I

S
|
S

G
|
G

G

G

F

F

R

R

N

S

A

I

T

V

S

E

Y

H

A

V

K

A

E

S

I

K

L

L

G

N

F

I

D

P

A

A

D

-

F

-

S

T

N

N

I

V

L

F

H

A

S

N

K

R

-

L

-

K

-

F

-

Y

-

F

N

N

G

G

R

E

L

Y

T

A

G

G

L

F

V

D

G

E

G

T

E

Q

M

P

M

T

F

A

-

E

S

S

H

G

S

G

K
|
K

G

G

D

K

M

V

L

I

K

K

R

L

L

L

Q

K

A

E

I

-

L

-

G

K

I

F

S

H

I

F

E

K

D

K

T

I

L

I

V

M

V

I

G

G

D
|
D

G

G

A

A

N

T

D

D

L

M

S

E

M

A

F

C

K

P

Y

P

A

A

G

D

T

A

R

F

I

I

A

G

F

F

binding magnesium ion: D16 (= D7), D18 (= D9), D175 (= D163)
binding serine: D16 (= D7), V17 (≠ F8), D18 (= D9), S105 (= S95), G106 (= G96), K154 (= K140)

6q6jB Human phosphoserine phosphatase with substrate analogue homo-cysteic acid (see paper)
29% identity, 85% coverage: 6:180/206 of query aligns to 15:192/217 of 6q6jB

query
sites
6q6jB

F

F

D
|
D

F
x
V

D
|
D

S

S

T

T

L

V

M

I

D

R

G

E

E

E

T

G

I

I

D

D

F

E

L

L

A

A

E

K

P

I

L

C

G

G

F

V

K

E

D

D

K

A

V

V

A

S

S

E

I

M

T

T

E

R

M

R

A

A

M

M

R

G

G

G

E

A

L

V

D

P

F
|
F

F

K

E

A

S

A

L

L

I

T

M

E

R

R

V

L

K

A

L

L

L

I

E

Q

G

P

L

S

E

R

D

E

K

Q

K

V

V

Q

N

R

E

L

I

I

C

A

H

E

N

Q

L

P

P

P

Y

H

M

L

-

T

P

P

G

G

A

I

D

R

E

E

T

L

I

V

K

S

A

R

L

L

K

Q

K

E

D

R

G

N

Y

V

K

Q

V

V

V

F

V

L

F

I

S
|
S

G
|
G

F

F

R

R

N

S

A

I

T

V

S

E

Y

H

A

V

K

A

E

S

I

K

L

L

G

N

F

I

D

P

A

A

D

-

F

-

S

T

N

N

I

V

L

F

H

A

S

N

K

R

-

L

-

K

-

F

-

Y

-

F

N

N

G

G

R

E

L

Y

T

A

G

G

L

F

V

D

G

E

G

T

E

Q

M

P

M

T

F

A

-

E

S

S

H

G

S

G

K
|
K

G

G

D

K

M

V

L

I

K

K

R

L

L

L

Q

K

A

E

I

-

L

-

G

K

I

F

S

H

I

F

E

K

D

K

T

I

L

I

V

M

V

I

G

G

D
|
D

G

G

A

A

N
x
T

D

D

L

M

S

E

M

A

F

C

K

P

Y

P

A

A

G

D

T

A

R

F

I

I

A

G

F

F

binding calcium ion: D16 (= D7), D18 (= D9), D175 (= D163)
binding (2~{S})-2-azanyl-4-sulfo-butanoic acid: D16 (= D7), V17 (≠ F8), D18 (= D9), F54 (= F45), S105 (= S95), G106 (= G96), G107 (= G97), K154 (= K140), T178 (≠ N166)

6hyjB Psph human phosphoserine phosphatase (see paper)
29% identity, 85% coverage: 6:180/206 of query aligns to 19:196/223 of 6hyjB

query
sites
6hyjB

F

F

D
|
D

F

V

D
|
D

S

S

T

T

L

V

M

I

D

R

G

E

E
|
E

T

G

I

I

D

D

F

E

L

L

A

A

E

K

P

I

L

C

G

G

F

V

K

E

D

D

K

A

V

V

A

S

S

E

I

M

T

T

E

R

M

R

A

A

M

M

R

G

G

G

E

A

L

V

D

P

F

F

F

K

E

A

S

A

L

L

I

T

M

E

R

R

V

L

K

A

L

L

L

I

E

Q

G

P

L

S

E

R

D

E

K

Q

K

V

V

Q

N

R

E

L

I

I

C

A

H

E

N

Q

L

P

P

P

Y

H

M

L

-

T

P

P

G

G

A

I

D

R

E

E

T

L

I

V

K

S

A

R

L

L

K

Q

K

E

D

R

G

N

Y

V

K

Q

V

V

V

F

V

L

F

I

S

S

G

G

F

F

R

R

N

S

A

I

T

V

S

E

Y

H

A

V

K

A

E

S

I

K

L

L

G

N

F

I

D

P

A

A

D

-

F

-

S

T

N

N

I

V

L
x
F

H

A

S

N

K

R

-

L

-

K

-

F

-

Y

-

F

N

N

G

G

R

E

L

Y

T

A

G

G

L

F

V

D

G

E

G

T

E

Q

M

P

M

T

F

A

-

E

S

S

H

G

S

G

K

K

G

G

D

K

M

V

L

I

K

K

R

L

L

L

Q

K

A

E

I

-

L

-

G
x
K

I

F

S

H

I

F

E

K

D

K

T

I

L

I

V

M

V

I

G

G

D
|
D

G

G

A

A

N

T

D

D

L

M

S

E

M

A

F

C

K

P

Y

P

A

A

G

D

T

A

R

F

I

I

A

G

F

F

binding calcium ion: D20 (= D7), D22 (= D9), D179 (= D163)
binding phosphoserine: F131 (≠ L119), K168 (≠ G152)
binding serine: E29 (= E16)

Sites not aligning to the query:

binding serine: 202

4ap9A Crystal structure of phosphoserine phosphatase from t.Onnurineus in complex with ndsb-201 (see paper)
29% identity, 100% coverage: 2:206/206 of query aligns to 10:196/200 of 4ap9A

query
sites
4ap9A

K

K

L

V

A

A

V

V

F

I

D
|
D

F
x
I

D
x
E

S

G

T

T

L

L

M

T

D

D

G

F

E

E

T

F

I

W

D

R

F

E

L

M

A

A

E
x
R

P
x
I

L
x
T

G

G

F

-

K

K

D

R

K

E

V

I

A

E

S

E

I

L

T

L

E

E

M

K

A

G

M
x
L

R

S

G

G

E

E

L

V

D

E

F
x
W

F

L

E

D

S

S

L

L

I

L

M

K

R

R

V

V

K

G

L

L

L

I

E

R

G

G

L

I

E

D

D

E

K

G

K

T

V

F

N

L

E

R

I

T

C

R

H

E

N

K

L

V

P

N

Y

V

M

S

P

P

G

E

A

A

D

R

E

E

T

L

I

V

K

E

A

T

L

L

K

R

K

E

D

K

G

G

Y

F

K

K

V

V

V

L

F

I

S

S

G
|
G

G

S

F

F

R

E

N

E

A

V

T

L

S

E

Y

P

A

F

K

K

E

E

I

-

L

L

G

G

F

-

D

D

A

E

D

F

F

M

S

A

N

N

I

R

L

A

H

I

S

F

K

E

N

D

G

G

R

K

L

F

T

Q

G

G

L

I

V

-

G

-

E

-

M

R

M

L

F

R

S

F

H

R

S
x
D

K
|
K

G

G

D

E

M

F

L

L

K

K

R

R

L

F

Q

R

A

D

I

G

L

F

G

-

I

-

S

-

I

-

E

-

D

-

T

I

L

L

V

A

V

M

G

G

D

D

G

G

A
x
Y

N
x
A

D
|
D

L

A

S

K

M

M

F

F

K

E

Y

R

A

A

G

D

T

M

R

G

I

I

A

A

F

-

C

V

A

G

K

R

D

E

V

I

L

-

K

-

K

P

E

G

A

A

N

D

V

L

I

L

I

V

E

-

K

K

D

D

L

L

T

K

K

E

I

L

L

V

E

D

F

F

I

I

active site: D15 (= D7), I16 (≠ F8), E17 (≠ D9), G103 (= G96), K141 (= K140), D162 (= D167)
binding 3-pyridinium-1-ylpropane-1-sulfonate: R31 (≠ E23), I32 (≠ P24), T33 (≠ L25), L46 (≠ M39), W52 (≠ F45), D140 (≠ S139), K141 (= K140), Y160 (≠ A165), A161 (≠ N166)

P78330 Phosphoserine phosphatase; PSP; PSPase; L-3-phosphoserine phosphatase; O-phosphoserine phosphohydrolase; EC 3.1.3.3 from Homo sapiens (Human) (see 4 papers)
29% identity, 85% coverage: 6:180/206 of query aligns to 19:196/225 of P78330

query
sites
P78330

F

F

D
|
D

F
x
V

D
|
D

S
|
S

T

T

L

V

M

I

D

R

G

E

E
|
E

T

G

I

I

D
|
D

F

E

L

L

A
|
A

E

K

P

I

L

C

G

G

F

V

K

E

D

D

K

A

V

V

A

S

S

E

I

M

T

T

E

R

M

R

A

A

M
|
M

R
x
G

G

G

E

A

L

V

D

P

F

F

F

K

E

A

S

A

L

L

I

T

M

E

R
|
R

V

L

K

A

L

L

L

I

E

Q

G

P

L

S

E

R

D

E

K

Q

K

V

V

Q

N

R

E

L

I

I

C

A

H

E

N

Q

L

P

P

P

Y

H

M

L

-

T

P

P

G

G

A

I

D

R

E

E

T

L

I

V

K

S

A

R

L

L

K

Q

K

E

D

R

G

N

Y

V

K

Q

V

V

V

F

V

L

F

I

S
|
S

G
|
G

F

F

R

R

N

S

A

I

T

V

S

E

Y

H

A

V

K

A

E

S

I

K

L

L

G

N

F

I

D

P

A

A

D

-

F

-

S

T

N

N

I

V

L

F

H

A

S
x
N

K

R

-

L

-

K

-

F

-

Y

-

F

N

N

G

G

R

E

L

Y

T

A

G

G

L

F

V

D

G

E

G

T

E

Q

M

P

M

T

F

A

-

E

S

S

H

G

S

G

K
|
K

G

G

D

K

M

V

L

I

K

K

R

L

L

L

Q

K

A

E

I

-

L

-

G

K

I

F

S

H

I

F

E

K

D

K

T

I

L

I

V

M

V

I

G

G

D
|
D

G

G

A

A

N
x
T

D

D

L

M

S

E

M

A

F

C

K

P

Y

P

A

A

G

D

T

A

R

F

I

I

A

G

F

F

D20 (= D7) binding Mg(2+)
DVD 20:22 (≠ DFD 7:9) binding L-serine
D22 (= D9) binding Mg(2+)
S23 (= S10) mutation to A: Reduces L-phosphoserine phosphatase activity by about 50%.; mutation to T: Reduces L-phosphoserine phosphatase activity by about 80%.
E29 (= E16) mutation to D: Reduces L-phosphoserine phosphatase activity by about 95%.; mutation to Q: Loss of L-phosphoserine phosphatase activity.
D32 (= D19) to N: in PSPHD; decreased L-phosphoserine phosphatase activity; dbSNP:rs104894035
A35 (= A22) to T: in PSPHD; decreased L-phosphoserine phosphatase activity
M52 (= M39) binding O-phospho-L-serine; to T: in PSPHD; decreased L-phosphoserine phosphatase activity; dbSNP:rs104894036
G53 (≠ R40) binding phosphate
R65 (= R52) mutation R->A,K: Loss of L-phosphoserine phosphatase activity.
SGG 109:111 (= SGG 95:97) binding L-serine; binding O-phospho-L-serine
N133 (≠ S121) mutation to A: Reduces L-phosphoserine phosphatase activity by about 75%.
K158 (= K140) binding L-serine; binding O-phospho-L-serine
D179 (= D163) binding Mg(2+)
T182 (≠ N166) binding O-phospho-L-serine; binding phosphate; mutation to S: Reduces L-phosphoserine phosphatase activity by about 99%.; mutation to V: Reduces L-phosphoserine phosphatase activity by about 25%.

Sites not aligning to the query:

202 R→A: Reduces L-phosphoserine phosphatase activity by about 99%.; R→K: Reduces L-phosphoserine phosphatase activity by about 95%.

1l8oA Molecular basis for the local conformational rearrangement of human phosphoserine phosphatase (see paper)
29% identity, 85% coverage: 6:180/206 of query aligns to 16:193/222 of 1l8oA

query
sites
1l8oA

F

F

D
|
D

F
x
V

D
|
D

S

S

T

T

L

V

M

I

D

R

G

E

E

E

T

G

I

I

D

D

F

E

L

L

A

A

E

K

P

I

L

C

G

G

F

V

K

E

D

D

K

A

V

V

A

S

S

E

I

M

T

T

E

R

M

R

A

A

M

M

R

G

G

G

E

A

L

V

D

P

F

F

F

K

E

A

S

A

L

L

I

T

M

E

R

R

V

L

K

A

L

L

L

I

E

Q

G

P

L

S

E

R

D

E

K

Q

K

V

V

Q

N

R

E

L

I

I

C

A

H

E

N

Q

L

P

P

P

Y

H

M

L

-

T

P

P

G

G

A

I

D

R

E

E

T

L

I

V

K

S

A

R

L

L

K

Q

K

E

D

R

G

N

Y

V

K

Q

V

V

V

F

V

L

F

I

S
|
S

G
|
G

G

G

F

F

R

R

N

S

A

I

T

V

S

E

Y

H

A

V

K

A

E

S

I

K

L

L

G

N

F

I

D

P

A

A

D

-

F

-

S

T

N

N

I

V

L

F

H

A

S

N

K

R

-

L

-

K

-

F

-

Y

-

F

N

N

G

G

R

E

L

Y

T

A

G

G

L

F

V

D

G

E

G

T

E

Q

M

P

M

T

F

A

-

E

S

S

H

G

S

G

K
|
K

G

G

D

K

M

V

L

I

K

K

R

F

L

L

Q

K

A

E

I

-

L

-

G

K

I

F

S

H

I

F

E

K

D

K

T

I

L

I

V

M

V

I

G

G

D

D

G
|
G

A

A

N
x
T

D
|
D

L

M

S

E

M

A

F

C

K

P

Y

P

A

A

G

D

T

A

R

F

I

I

A

G

F

F

active site: D17 (= D7), V18 (≠ F8), D19 (= D9), G107 (= G96), K155 (= K140), D180 (= D167)
binding phosphate ion: D17 (= D7), D19 (= D9), S106 (= S95), K155 (= K140)
binding serine: G177 (= G164), T179 (≠ N166)

Sites not aligning to the query:

binding serine: 199

1l8lA Molecular basis for the local confomational rearrangement of human phosphoserine phosphatase (see paper)
29% identity, 85% coverage: 6:180/206 of query aligns to 16:193/222 of 1l8lA

query
sites
1l8lA

F

F

D
|
D

F
x
V

D
|
D

S

S

T

T

L

V

M

I

D

R

G

E

E

E

T

G

I

I

D

D

F

E

L

L

A

A

E

K

P

I

L

C

G

G

F

V

K

E

D

D

K

A

V

V

A

S

S

E

I

M

T

T

E

R

M

R

A

A

M

M

R

G

G

G

E

A

L

V

D

P

F

F

F

K

E

A

S

A

L

L

I

T

M

E

R

R

V

L

K

A

L

L

L

I

E

Q

G

P

L

S

E

R

D

E

K

Q

K

V

V

Q

N

R

E

L

I

I

C

A

H

E

N

Q

L

P

P

P

Y

H

M

L

-

T

P

P

G

G

A

I

D

R

E

E

T

L

I

V

K

S

A

R

L

L

K

Q

K

E

D

R

G

N

Y

V

K

Q

V

V

V

F

V

L

F

I

S

S

G
|
G

G

G

F

F

R

R

N

S

A

I

T

V

S

E

Y

H

A

V

K

A

E

S

I

K

L

L

G

N

F

I

D

P

A

A

D

-

F

-

S

T

N

N

I

V

L

F

H

A

S

N

K

R

-

L

-

K

-

F

-

Y

-

F

N

N

G

G

R

E

L

Y

T

A

G

G

L

F

V

D

G

E

G

T

E

Q

M

P

M

T

F

A

-

E

S

S

H

G

S

G

K
|
K

G

G

D

K

M

V

L

I

K

K

R

F

L

L

Q

K

A

E

I

-

L

-

G

K

I

F

S

H

I

F

E

K

D

K

T

I

L

I

V

M

V

I

G

G

D
|
D

G
|
G

A

A

N
x
T

D
|
D

L

M

S

E

M

A

F

C

K

P

Y

P

A

A

G

D

T

A

R

F

I

I

A

G

F

F

active site: D17 (= D7), V18 (≠ F8), D19 (= D9), G107 (= G96), K155 (= K140), D180 (= D167)
binding d-2-amino-3-phosphono-propionic acid: D17 (= D7), D19 (= D9), G107 (= G96), K155 (= K140), D176 (= D163), G177 (= G164), T179 (≠ N166)

3kd3A Crystal structure of a phosphoserine phosphohydrolase-like protein from francisella tularensis subsp. Tularensis schu s4
28% identity, 87% coverage: 1:180/206 of query aligns to 1:188/216 of 3kd3A

query
sites
3kd3A

M

M

K

K

L

N

A

I

V

I

F

F

D
|
D

F
|
F

D
|
D

S

S

T

T

L

L

M

I

D

K

G

K

E

E

T

S

I

L

D

E

F

L

L

I

A

L

E

E

P

P

L

I

G

L

F

Q

K

K

D

S

-

P

-

A

-

K

-

L

-

K

K

E

V

I

A

E

S

Y

I

I

T

T

E

N

M

L

A

G

M

M

R

Q

G

G

E

D

L

I

D

S

F

F

F

R

E

D

S

S

L

L

I

Q

M

K

R

R

V

L

K

A

L

I

L

A

E

S

G

P

L

T

E

K

D

Q

-

S

-

I

-

K

K

E

K

F

V

S

N

N

E

K

I

Y

C

C

H

P

N

N

L

L

P

-

Y

L

M

T

P

D

G

G

A

I

D

K

E

E

T

L

I

V

K

Q

A

D

L

L

K

K

K

N

D

K

G

G

Y

F

K

E

V

I

V

W

V

I

F

F

S

S

G
|
G

G

G

F

L

R

-

N

-

A

-

T

-

S

-

Y

-

A

S

K

E

E

S

I

I

L

Q

G

P

F

F

D

-

A

A

D

D

F

Y

S

L

N

N

I

I

L

P

H

R

S

E

K

N

N

I

G

F

R

A

L

V

T

E

G

T

L

I

V

W

G

N

G

S

E

D

M

G

M

S

F

F

S

K

-

E

-

L

-

D

H

N

S

S

K

N

G

G

D

A

M

C

L

D

K

S

R

K

L

L

Q

S

A

A

I

F

-

D

-

K

-

A

L

K

G

G

I

L

S

I

I

D

E

G

D

E

T

V

L

I

V

A

V

I

G

G

D
|
D

G

G

A

Y

N

T

D
|
D

L

Y

S

Q

M

L

F

Y

K

E

-

K

-

G

Y

Y

A

A

G

T

T

K

R

F

I

I

A

A

F

Y

active site: D7 (= D7), F8 (= F8), D9 (= D9), G103 (= G96), D173 (= D167)
binding magnesium ion: D7 (= D7), D9 (= D9), D169 (= D163)

Query Sequence

>WP_007474112.1 NCBI__GCF_000170735.1:WP_007474112.1
MKLAVFDFDSTLMDGETIDFLAEPLGFKDKVASITEMAMRGELDFFESLIMRVKLLEGLE
DKKVNEICHNLPYMPGADETIKALKKDGYKVVVFSGGFRNATSYAKEILGFDADFSNILH
SKNGRLTGLVGGEMMFSHSKGDMLKRLQAILGISIEDTLVVGDGANDLSMFKYAGTRIAF
CAKDVLKKEANVIIEEKDLTKILEFI

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory